Patent Application
20100137324
The present invention relates to compounds and derivatives thereof, their synthesis, and their use as Rho-kinase inhibitors. These compounds of the present invention are useful for inhibiting tumor growth, treating erectile dysfunction, and treating other indications mediated by Rho-kinase, e.g., coronary heart disease.
The pathology of a number of human and animal diseases including hypertension, erectile dysfunction, coronary cerebral circulatory impairments, neurodegenerative disorders and cancer can be linked directly to changes in the actin cytoskeleton. These diseases pose a serious unmet medical need. The actin cytoskeleton is composed of a meshwork of actin filaments and actin-binding proteins found in all eukaryotic cells. In smooth muscle cells the assembly and disassembly of the actin cytoskeleton is the primary motor force responsible for smooth muscle contraction and relaxation. In non-muscle cells, dynamic rearrangements of the actin cytoskeleton are responsible for regulating cell morphology, cell motility, actin stress fiber formation, cell adhesion and specialized cellular functions such as neurite retraction, phagocytosis or cytokinesis (Van Aelst, et al. Genes Dev 1997, 11, 2295).
The actin cytoskeleton is controlled by a family of proteins that are a subset of the Ras superfamily of GTPases. This subset currently consists of RhoA through E and RhoG (refereed to collectively as Rho), Rac 1 and 2, Cdc42Hs and G25K and TC10 isoforms (Mackay, et al. J Biol Chem 1998, 273, 20685). These proteins are GTP (guanine nucleotide triphosphate) binding proteins with intrinsic GTPase activity. They act as molecular switches and cycles between inactive GDP (guanine nucleotide diphosphate) bound and active GTP bound states. Using biochemical and genetic manipulations, it has been possible to assign functions to each family member. Upon activation the Rho proteins controls the formation of actin stress fibers, thick bundles of actin filaments, and the clustering of integrins at focal adhesion complexes. When activated the Rac proteins control the formation of lamellopodia or membrane ruffles on the cell surface and Cdc42 controls filopodia formation. Together this family of proteins plays a critical part in the control of key cellular functions including cell movement, axonal guidance, cytokinesis, and changes in cell morphology, shape and polarity.
Depending on the cell type and the activating receptor, the Rho proteins can control different biological responses. In smooth muscle cells, Rho proteins are responsible for the calcium sensitization during smooth muscle contraction. In non-smooth muscle cells the Rho GTPases are responsible for the cellular responses to agonist such as lysophosphatidic acid (LPA), thrombin and thromboxane A2 (Fukata, et al. Trends Pharcol Sci 2001, 22, 32). Agonist response is coupled through heterotrimeric G proteins Galpha12 or Galpha13 (Goetzl, et al. Cancer Res 1999, 59, 4732; Buhl, et al. J Biol Chem 1995, 270, 24631) though other receptors may be involved. Upon activation Rho GTPases activate a number of downstream effectors including PIPS-kinase, Rhothekin, Rhophilin, PKN and Rho-Kinase isoforms ROCK-1/ROKbeta and ROCK-1/ROKalpha (Mackay and Hall J Biol Chem 1998, 273, 20685; Aspenstrom Curr Opin Cell Biol 1999, 11, 95; Amano, et al. Exp Cell Res 2000, 261, 44).
Rho-kinase was identified as a RhoA interacting protein isolated from bovine brain (Matsui, et al. Embo J 1996, 15, 2208). It is a member of the myotonic dystrophy family of protein kinase and contains a serine/threonine kinase domain at the amino terminus, a coiled-coil domain in the central region and a Rho interaction domain at the carboxy terminus (Amano, et al. Exp Cell Res 2000, 261, 44). Its kinase activity is enhanced upon binding to GTP-bound RhoA and when introduced into cells, it can reproduce many of the activities of activated RhoA. In smooth muscle cells Rho-Kinase mediates calcium sensitization and smooth muscle contraction and inhibition of Rho-kinase blocks 5-HT and phenylephrine agonist induced muscle contraction. When introduced into non-smooth muscle cells, Rho-kinase induces stress fiber formation and is required for the cellular transformation mediated by RhoA (Sahai, et al. Curr Biol 1999, 9, 136). Rho-kinase regulates a number of downstream proteins through phosphorylation, including myosin light chain (Somlyo, et al. J Physiol (Lond) 2000, 522 Pt 2, 177), the myosin light chain phosphatase binding subunit (Fukata, et al. J Cell Biol 1998, 141, 409) and LIM-kinase 2 (Sumi, et al. J Bio Chem 2001, 276, 670).
Inhibition of Rho-kinase activity in animal models has demonstrated a number of benefits of Rho-kinase inhibitors for the treatment of human diseases. Several patents have appeared claiming (+)-trans-4-(1-aminoethyl)-1-(pyridin-4-ylaminocarbonyl)cyclohexane dihydrochloride monohydrate (WO-00078351, WO-00057913) and substituted isoquinolinesulfonyl (EP-00187371) compounds as Rho-kinase inhibitors with activity in animal models. These include models of cardiovascular diseases such as hypertension (Uehata, et al. Nature 1997, 389, 990), atherosclerosis (Retzer, et al. FEBS Lett 2000, 466, 70), restenosis (Eto, et al. Am J Physiol Heart Circ Physiol 2000, 278, H1744; Negoro, et al. Biochem Biophys Res Commun 1999, 262, 211), cerebral ischemia (Uehata, et al. Nature 1997, 389, 990; Seasholtz, et al. Circ Res 1999, 84, 1186; Hitomi, et al. Life Sci 2000, 67, 1929; Yamamoto, et al. J Cardiovasc Pharmacol 2000, 35, 203), cerebral vasospasm (Sato, et al. Circ Res 2000, 87, 195; Kim, et al. Neurosurgery 2000, 46, 440), penile erectile dysfunction (Chitaley, et al. Nat Med 2001, 7, 119), central nervous system disorders such as neuronal degeneration and spinal cord injury (Hara, et al. J Neurosurg 2000, 93, 94; Toshima, et al. Stroke 2000, 31, 2245) and in neoplasias where inhibition of Rho-kinase has been shown to inhibit tumor cell growth and metastasis (Itoh, et al. Nat Med 1999, 5, 221; Somlyo, et al. Biochem Biophys Res Commun 2000, 269, 652), angiogenesis (Uchida, et al. Biochem Biophys Res Commun 2000, 269, 633; Gingras, et al. Biochem J 2000, 348 Pt 2, 273), arterial thrombotic disorders such as platelet aggregation (Klages, et al. J Cell Biol 1999, 144, 745; Retzer, et al. Cell Signal 2000, 12, 645) and leukocyte aggregation (Kawaguchi, et al. Eur J Pharmacol 2000, 403, 203; Sanchez-Madrid, et al. Embo J 1999, 18, 501), asthma (Setoguchi, et al. Br J Pharmacol 2001, 132, 111; Nakahara, et al. Eur J Pharmacol 2000, 389, 103), regulation of intraoccular pressure (Honjo, et al. Invest Opthalmol Vis Sci 2001, 42, 137) and bone resorption (Chellaiah, et al. J Biol Chem 2000, 275, 11993; Zhang, et al. J Cell Sci 1995, 108, 2285).
The inhibition of Rho-kinase activity in patients has benefits for controlling cerebral vasospasms and ischemia following subarachnoid hemorrhage (Pharma Japan 1995, 1470, 16).
The compounds and their derivatives presented in this invention are useful as Rho-Kinase inhibitors and thus have utilities in the treatment of hypertension, atherosclerosis, restenosis, cerebral ischemia, cerebral vasospasm, neuronal degeneration, spinal cord injury, cancers of the breast, colon, prostate, ovaries, brain and lung and their metastases, thrombotic disorders, asthma, glaucoma and osteoporosis.
In addition, the compounds of the invention are useful to treat erectile dysfunction, i.e., erectile dysfunction mediated by Rho-kinase. Erectile dysfunction can be defined as an inability to obtain or sustain an erection adequate for intercourse, WO 94/28902, U.S. Pat. No. 6,103,765 and U.S. Pat. No. 6,124,461.
The invention provides compounds of Formula I
wherein Y is ═N— or ═CR17,
X is —(CH2)x—, —O—(CH2)n—, —S—(CH2)n—, —NR7—CO—(CH2)n—,
—NR7—SO2—(CH2)n—, —NR7—(CH2)n—, or —(O)C—NR7—,
each n is an integer which is independently 0, 1, 2 or 3,
x is 0-3
p is 0-3
a and c are each independently —CR5=, —N═, or —NR6-, wherein one of a or c is —NR6-, and b is —CR5= or —N═;
A is H, halogen, —CO—OR8, —CO—R8, cyano, —OR8, —NR8R9, —CO—NR8R9, —NR8—CO—R9, —NR8—CO—OR9, —NR8—SO2—R9, —SR8, —SO2—R8, —SO2—NR8R9, NR8—CO—NHR9,
or
A is a 3-20 atom, preferably 5-15 atom, cyclic or polycyclic moiety, e.g., containing 1-4 rings, which optionally contain 1-3 N, O or S atoms per ring, and may optionally be aryl or heteroaryl. A may optionally be substituted up to 3 times by (i) C1-C10 alkyl or C2-C10-alkenyl, each optionally substituted with halogen up to perhalo; (ii) C3-C10 cycloalkyl; (iii) aryl; (iv) heteroaryl; (v) halogen; (vi) —CO—OR8; (vii) —CO—R8; (viii) cyano; (ix) —OR8, (x) (x) —NR8R13; (xi) nitro; (xii) —CO—NR8R9; (xiii) —C1-10-alkyl-NR8R9; (xiv) —NR8—CO—R12; (xv) —NR8—CO—OR9; (xvi) —NR8—SO2—R9; (xvii) —SR8; (xviii) —SO2—R8; (xix) —SO2—NR8R9; or (xx) NR8—CO—NHR9;
Ring B is optionally independently substituted up to 3 times in any position by R5
R1, and R6-R11 are each independently hydrogen or C1-6 alkyl,
R2-R5 are each independently (i) hydrogen, (ii) C1-10 alkyl or C2-10-alkenyl each optionally substituted by amino, N-lower alkylamino, N,N-dilower alkylamino, N-lower alkanoylamino, hydroxy, cyano, —COOR10, —COR14, —OCOR14, —OR10, C5-10-heteroaryl, C5-10-heteroaryloxy, or C5-10-heteroaryl-C1-10-alkoxy, halogen up to perhalo; (iii) C3-C10 cycloalkyl, in which 1-3 carbon atoms are optionally independently replaced by O, N or S; (iv) C3-10-cycloalkenyl; (v) partially unsaturated C5-10-heterocyclyl; (vi) aryl; (vii) heteroaryl; (viii) halogen; (ix) —CO—OR10;
(x) —OCOR10; (xi) —OCO2R10; (xii) —CHO; (xiii) cyano; (xiv) —OR16; (xv) —NR10R15; (xvi) nitro; (xvii) —CO—NR10R11; (xviii) —NR10—CO—R12; (xix) —NR10—CO—OR11; (xx) —NR10—SO2—R12; (xxi) —SR16; (xxii) —SOR16; (xxiii) —SO2—R16; (xxiv) —SO2—NR10R11; (xxv) NR10—CO—NHR11; (xxvi) amidino; (xxvii) guanidino;
(xxviii) sulfo; (xxix) —B(OH)2; (xxx) —OCON(R10)2; or (xxxi) —NR10CON(R10)2; and R5 in a, b or c is preferably hydrogen or C1-10-alkyl or C2-10-alkyl optionally substituted as above, more preferably hydrogen or C1-10-alkyl,
R12 is H, C1-6-alkyl or C5-10-aryl,
R13 is H, C1-6-alkyl or C1-6-alkoxy,
R14 is lower alkyl or phenyl;
R15 is lower alkyl, halogen, amino, N-lower alkyl amino, N,N-dilower alkylamino, N-lower alkanoylamino, OH, CN, COOR10, —COR14 or —OCOR14;
R16 is hydrogen, C1-6-alkyl optionally substituted by halogen, up to perhalo, or C5-10-heteroaryl; and
R17 is H, C1-6 alkyl or CN,
with the provisos that A is not hydrogen when x is 0, and that Formula I is not
Suitable alkyl groups and alkyl portions of groups, e.g., alkoxy, etc. throughout include methyl, ethyl, propyl, butyl, etc., including all straight-chain and branched isomers such as isopropyl, isobutyl, sec-butyl, tert-butyl, etc.
Suitable cycloalkyl groups include cyclopropyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, etc.
Suitable halogen groups include F, Cl, Br, and/or I, from one to per-substitution (i.e., all H atoms on a group replaced by a halogen atom) being possible, mixed substitution of halogen atom types also being possible on a given moiety.
In Formula I, suitable aryl or heteroaryl groups, e.g., for A, include, but are not limited to, 5-12 carbon-atom aromatic rings or ring systems containing 1-3 rings, at least one of which is aromatic, in which one or more, e.g., 1-4 carbon atoms in one or more of the rings can be replaced by oxygen, nitrogen or sulfur atoms. Each ring typically has 3-7 atoms. For example, aryl or heteroaryl can be 2- or 3-furyl, 2- or 3-thienyl, 2- or 4-triazinyl, 1-, 2- or 3-pyrrolyl, 1-, 2-, 4- or 5-imidazolyl, 1-, 3-, 4- or 5-pyrazolyl, 2-, 4- or 5-oxazolyl, 3-, 4- or 5-isoxazolyl, 2-, 4- or 5-thiazolyl, 3-, 4- or 5-isothiazolyl, 2-, 3- or 4-pyridyl, 2-, 4-, 5- or 6-pyrimidinyl, 1,2,3-triazol-1-, -4- or 5-yl, 1,2,4-triazol-1-, -3- or 5-yl, 1- or 5-tetrazolyl, 1,2,3-oxadiazol-4- or 5-yl, 1,2,4-oxadiazol-3- or 5-yl, 1,3,4-thiadiazol-2- or 5-yl, 1,2,4-oxadiazol-3- or 5-yl, 1,3,4-thiadiazol-2- or 5-yl, 1,3,4-thiadiazol-3- or 5-yl, 1,2,3-thiadiazol-4- or 5-yl, 2-, 3-, 4-, 5- or 6-2H-thiopyranyl, 2-, 3- or 4-4H-thiopyranyl, 3- or 4-pyridazinyl, pyrazinyl, 2-, 3-, 4-, 5-, 6- or 7-benzofuryl, 2-, 3-, 4-, 5-, 6- or 7-benzothienyl, 1-, 2-, 3-, 4-, 5-, 6- or 7-indolyl, 1-, 2-, 4- or 5-benzimidazolyl, 1-, 3-, 4-, 5-, 6- or 7-benzopyrazolyl, 2-, 4-, 5-, 6- or 7-benzoxazolyl, 3-, 4-, 5- 6- or 7-benzisoxazolyl, 1-, 3-, 4-, 5-, 6- or 7-benzothiazolyl, 2-, 4-, 5-, 6- or 7-benzisothiazolyl, 2-, 4-, 5-, 6- or 7-benz-1,3-oxadiazolyl, 2-, 3-, 4-, 5-, 6-, 7- or 8-quinolinyl, 1-, 3-, 4-, 5-, 6-, 7-, 8-isoquinolinyl, 1-, 2-, 3-, 4- or 9-carbazolyl, 1-, 2-, 3-, 4-, 5-, 6-, 7-, 8- or 9-acridinyl, or 2-, 4-, 5-, 6-, 7- or 8-quinazolinyl, or additionally optionally substituted phenyl, 2- or 3-thienyl, 1,3,4-thiadiazolyl, 3-pyrryl, 3-pyrazolyl, 2-thiazolyl or 5-thiazolyl, etc.
Preferred moieties A include cyclohexyl; or C5-12-aryl or C5-12-heteroaryl each independently optionally substituted up to three times by (i) C1-C10-alkyl or C2-10-alkenyl each optionally substituted with halogen up to perhalo; (ii) C3-C10 cycloalkyl; (iii) C5-12-aryl optionally substituted by 1-3 halogen atoms; (iv) C5-12-heteroaryl; (v) halogen; (vi) —CO—OR8; (vii) —CO—R8; (viii) cyano; (ix) —OR8; (x) —NR8R13; (xi) nitro; (xii) —CO—NR8R9; (xiii) —C1-10-alkyl-NR8R9; (xiv) —NR8—CO—R12; (xv) —NR8—CO—OR9; (xvi) —NR8—SO2—R9; (xvii) —SR8; (xviii) —SO2—R8; (xix) —SO2—NR8R9, or (xx) NR8—CO—NHR9.
Further preferred moieties A include phenyl, pyridyl, pyrimidinyl, oxazolyl, furyl, thienyl, pyrrolyl, imidazolyl, isoxazolyl and pyrazinyl, each independently substituted up to three times by halogen, C1-10-alkyl, C1-10-alkoxyphenyl, naphthyl, —OR10,
wherein each Z independently is halogen, hydroxy, hydroxy-C1-40-alkyl, —CN, —NO2, C1-10-alkoxycarboxyl, —NR10—CO—R11, or —NR10—CO—OR11,
y is 1-3,
and R4 is as described above
Preferred moieties A additionally include
wherein R15 is H; phenyl optionally substituted by C1-10-alkyl, C1-10-alkoxy, C1-10-alkylcarboxyl, or halogen; benzyl; pyramidal or pyridyl; and R16 is H, phenyl, —COOR10,
The present invention is also directed to pharmaceutically acceptable salts of Formula I. Suitable pharmaceutically acceptable salts are well known to those skilled in the art and include basic salts of inorganic and organic acids, such as hydrochloric acid, hydrobromic acid, sulphuric acid, phosphoric acid, methanesulphonic acid, sulphonic acid, acetic acid, trifluoroacetic acid, malic acid, tartaric acid, citric acid, lactic acid, oxalic acid, succinic acid, fumaric acid, maleic acid, benzoic acid, salicyclic acid, phenylacetic acid, and mandelic acid. In addition, pharmaceutically acceptable salts include acid salts of inorganic bases, such as salts containing alkaline cations (e.g., Li+, Na+ or K+), alkaline earth cations (e.g., Mg+, Ca+ or Ba+), the ammonium cation, as well as acid salts of organic bases, including aliphatic and aromatic substituted ammonium, and quaternary ammonium cations, such as those arising from protonation or peralkylation of triethylamine, N,N-diethylamine, N,N-dicyclohexylamine, pyridine, N,N-dimethylaminopyridine (DMAP), 1,4-diazabiclo[2.2.2]octane (DABCO), 1,5-diazabicyclo[4.3.0]non-5-ene (DBN) and 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU).
A number of the compounds of Formula I possess asymmetric carbons and can therefore exist in racemic and optically active forms. Methods of separation of enantiomeric and diastereomeric mixtures are well known to one skilled in the art. The present invention encompasses any isolated racemic or optically active form of compounds described in Formula I which possess Rho-kinase inhibitory activity.
The invention also includes pharmaceutical compositions including a compound of Formula I, and a physiologically acceptable carrier.
Preferred compounds include:
The invention moreover encompasses treating indications mediated by Rho-kinase, by administering a compound of Formula I, or a pharmaceutical composition containing a compound of Formula I. Thus, the invention encompasses treating cardiovascular diseases such as hypertension, artherosclerosis, restenosis and cerebral ischemia, or vasospasm central nervous system disorders such as neuronal degeneration and spinal cord injury, erectile dysfunction, e.g., in patients who do not have satisfactory response to PDE-5 inhibitors, and cancer (e.g., tumor growth) mediated by Rho-kinase, by administering, e.g., to a host in need thereof, of an effective amount of a compound of Formula I. Cancers and tumors mediated by Rho-kinase include cancers of the breast, colon, prostate, ovaries, brain and lung and their metastases.
The compounds may be administered orally, topically, parenterally, by inhalation or spray, vaginally, rectally or sublingually in dosage unit formulations. The term ‘administration by injection’ includes intravenous, intraarticular, intramuscular, subcutaneous and parenteral injections, as well as use of infusion techniques. Dermal administration may include topical application or transdermal administration. One or more compounds may be present in association with one or more non-toxic pharmaceutically acceptable carriers and if desired other active ingredients.
Compositions intended for oral use may be prepared according to any suitable method known to the art for the manufacture of pharmaceutical compositions. Such compositions may contain one or more agents selected from the group consisting of diluents, sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide palatable preparations. Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets. These excipients may be, for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; and binding agents, for example magnesium stearate, stearic acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and adsorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate may be employed. These compounds may also be prepared in solid, rapidly released form.
Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil.
Aqueous suspensions containing the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions may also be used. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydroxypropyl-methylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide, for example, lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethylene oxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.
Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, for example, sweetening, flavoring and coloring agents, may also be present.
The compounds may also be in the form of non-aqueous liquid formulations, e.g., oily suspensions which may be formulated by suspending the active ingredients in a vegetable oil, for example arachis oil, olive oil, sesame oil or peanut oil, or in a mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
Compounds of the invention may also be administrated transdermally using methods known to those skilled in the art (see, for example: Chien; “Transdermal Controlled Systemic Medications”; Marcel Dekker, Inc.; 1987. Lipp et al. WO94/04157 3Mar. 1994). For example, a solution or suspension of a compound of Formula I in a suitable volatile solvent optionally containing penetration enhancing agents can be combined with additional additives known to those skilled in the art, such as matrix materials and bacteriocides. After sterilization, the resulting mixture can be formulated following known procedures into dosage forms. In addition, on treatment with emulsifying agents and water, a solution or suspension of a compound of Formula I may be formulated into a lotion or salve.
Suitable solvents for processing transdermal delivery systems are known to those skilled in the art, and include lower alcohols such as ethanol or isopropyl alcohol, lower ketones such as acetone, lower carboxylic acid esters such as ethyl acetate, polar ethers such as tetrahydrofuran, lower hydrocarbons such as hexane, cyclohexane or benzene, or halogenated hydrocarbons such as dichloromethane, chloroform, trichlorotrifluoroethane, or trichlorofluoroethane. Suitable solvents may also include mixtures of one or more materials selected from lower alcohols, lower ketones, lower carboxylic acid esters, polar ethers, lower hydrocarbons, halogenated hydrocarbons.
Suitable penetration enhancing materials for transdermal delivery system are known to those skilled in the art, and include, for example, monohydroxy or polyhydroxy alcohols such as ethanol, propylene glycol or benzyl alcohol, saturated or unsaturated C8-C18 fatty alcohols such as lauryl alcohol or cetyl alcohol, saturated or unsaturated C8-C18 fatty acids such as stearic acid, saturated or unsaturated fatty esters with up to 24 carbons such as methyl, ethyl, propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tertbutyl or monoglycerin esters of acetic acid, capronic acid, lauric acid, myristinic acid, stearic acid, or palmitic acid, or diesters of saturated or unsaturated dicarboxylic acids with a total of up to 24 carbons such as diisopropyl adipate, diisobutyl adipate, diisopropyl sebacate, diisopropyl maleate, or diisopropyl fumarate. Additional penetration enhancing materials include phosphatidyl derivatives such as lecithin or cephalin, terpenes, amides, ketones, ureas and their derivatives, and ethers such as dimethyl isosorbid and diethyleneglycol monoethyl ether. Suitable penetration enhancing formulations may also include mixtures of one or more materials selected from monohydroxy or polyhydroxy alcohols, saturated or unsaturated C8-C18 fatty alcohols, saturated or unsaturated C8-C18 fatty acids, saturated or unsaturated fatty esters with up to 24 carbons, diesters of saturated or unsaturated discarboxylic acids with a total of up to 24 carbons, phosphatidyl derivatives, terpenes, amides, ketones, ureas and their derivatives, and ethers.
Suitable binding materials for transdermal delivery systems are known to those skilled in the art and include polyacrylates, silicones, polyurethanes, block polymers, styrenebutadiene copolymers, and natural and synthetic rubbers. Cellulose ethers, derivatized polyethylenes, and silicates may also be used as matrix components. Additional additives, such as viscous resins or oils may be added to increase the viscosity of the matrix.
Pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions. The oil phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example, liquid paraffin or mixtures of these. Suitable emulsifying agents may be naturally-occurring gums, for example, gum acacia or gum tragacanth, naturally-occurring phosphatides, for example, soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol anhydrides, for example, sorbitan monooleate, and condensation products of the said partial esters with ethylene oxide, for example, polyoxyethylene sorbitan monooleate. The emulsions may also contain sweetening and flavoring agents.
Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents.
The compounds may also be administered in the form of suppositories for rectal or vaginal administration of the drug. These compositions can be prepared by mixing the drug with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature or vaginal temperature and will therefore melt in the rectum or vagina to release the drug. Such materials include cocoa butter and polyethylene glycols.
Moreover, for treatment of erectile dysfunction, the present pharmaceutical compositions may take any form which is suitable for administration to the penis either via injection into the corpora cavernosa or transurethral administration, or topically applied to the urethral meatus. In the case of injection into the corpora cavernosa, the pharmaceutical composition is suitably in the form of a saline solution. Preferably, the pharmaceutical composition is in a form suitable for transurethral administration, and in this case the composition is typically in the form of a solution, an ointment, or a suppository. Typically, the pharmaceutical composition is administered 1 to 50 minutes, preferably 10 to 20 minutes, prior to the time of commencing sexual intercourse.
For all regimens of use disclosed herein for compounds of Formula I, the daily oral dosage regimen will preferably be from 0.01 to 200 mg/Kg of total body weight. The daily dosage for administration by injection, including intravenous, intramuscular, subcutaneous and parenteral injections, and use of infusion techniques will preferably be from 0.01 to 200 mg/Kg of total body weight. The daily vaginal dosage regime will preferably be from 0.01 to 200 mg/Kg of total body weight. The daily topical dosage regimen will preferably be from 0.01 to 200 mg administered between one to four times daily. The transdermal concentration will preferably be that required to maintain a daily dose is of from 0.1 to 200 mg/Kg. The daily inhalation dosage regimen will preferably be from 0.01 to 10 mg/Kg of total body weight.
It will be appreciated by those skilled in the art that the particular method of administration will depend on a variety of factors, all of which are considered routinely when administering therapeutics. It will also be understood, however, that the specific dose level for any given patient will depend upon a variety of factors, including, the activity of the specific compound employed, the age of the patient, the body weight of the patient, the general health of the patient, the gender of the patient, the diet of the patient, time of administration, route of administration, rate of excretion, drug combinations, and the severity of the condition undergoing therapy. It will be further appreciated by one skilled in the art that the optimal course of treatment, i.e., the mode of treatment and the daily number of doses of a compound of Formula I or a pharmaceutically acceptable salt thereof given for a defined number of days, can be ascertained by those skilled in the art using conventional treatment tests.
The present compounds and compositions exhibit Rho-kinase inhibitory activity, and are thus useful to treat the indications listed above, e.g., indications mediated by Rho-kinase. By indications mediated by Rho-kinase is meant diseases or conditions whose progression proceeds, at least in part, via the Rho pathway.
Rho-kinase inhibitory activity, e.g., ROCK-1 inhibition, can be evaluated as follows:
The kinase domain of human ROCK-1, amino acids 27-530, is isolated as a glutathione S-transferase fusion protein from Sf9 insect cells. The protein is partially purified by glutathione Sepharose 4B (Pharmacia Biotech, Piscataway, N.J.) affinity purification. Reactions is carried out in 96-well plates in a total volume of 100 uL containing 50 mM N-[2-Hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid] pH 7.5, 5 mM MgCl2, 1 mM dithiothreitol, 6 μM ATP, 0.2 μCi [33P]ATP (NEN, Boston, Mass.), 1 μg myelin basic protein and 0.1 μg ROCK-1. Test compounds are dissolved in 100% dimethylsulfoxide, diluted to the appropriated concentration and added to the reaction. The final concentration of dimethylsulfoxide did not exceed 0.5%. The reaction is run for one hour at room temperature. The reaction is stopped with the addition of 7 mL of 1 N HCL, transferred to P30 membranes and the amount of [33P]ATP, as counts per minute (c.p.m.) incorporated into the substrate, myelin basic protein, is read in a BetaPlate Reader (Packard Instrument Co., Meriden, Conn.). (All reagents were purchased from Sigma Chemical Co., St. Louis, Mo. unless stated otherwise.) Percentage inhibition is measured by the amount of incorporation of radioactivity in the presence of the test compound when compared to the amount of incorporation in the absence of the test compound.
Inhibitory activity can also be evaluated by measurement of stress fiber formation, performed essentially as described by Ridley, A. J., and A. Hall, Cell 70:389-399 (1992). Human fibrosarcoma HT1080 (CCL-121, American Type Culture Collection, Manassas, Va.) cells are plated on 22×22 mm #1 glass cover slips in six-well tissue culture plates (Costar) at 2.5×104 cells/well in Delbeco's modified Eagle's Medium (DMEM, Gibco) supplemented with 10% fetal calf serum. Cells are maintained in a humidified, 5% CO2 atmosphere at 37° C. After 24 hours the culture medium is removed and replaced with medium without 10% fetal calf serum and the cells cultured for an additional 48 hours. Test compounds are dissolved in 100% dimethylsulfoxide, diluted to the appropriated concentration and added to the culture medium 60 minutes prior to the induction of stress fiber formation. The final concentration of dimethylsulfoxide did not exceed 0.25%. Stress fiber formation is induced by the addition of lysophosphatidic acid (1-oleoyl-2-hydroxy-sn-glycerol-3-phosphate, Avanti Polar-Lipids, Alabaster, Al) to 10 μM final concentration in Delbeco's modified Eagle's Medium containing 0.1% fatty acid free bovine serum albumin for 15 minutes at 37° C. Cells are fixed with 4% paraformaldeyhde (Poly Scientific, Bay Shore, N.J.) in phosphate buffered saline (PBS) for 15 minutes. Cells are then washed 3 times in PBS and them permeabilized using a solution containing 40 mM piperazine-N—N′ bis[2-ethanesulfonic acid], 50 mM N-[2-hydroryethyl]piperaxine-N′-[2-ethanesulfonic acid], 0.1% Triton X-100, 75 mM NaCl, mM MgCl2, 0.5 mM EGTA, pH 7.2 for 2 minutes at room temperature. The cells are washed 3 times for 5 minutes each in PBS and then actin stress fibers are stained using 10 units/mL rhodamine phalloidin (Molecular Probes, Eugene, Oreg.) in PBS for 60 minutes at room temperature. The cells are washed 3 times with PBS and the cover slips mounted on glass microscope slides. The percentage of stress fiber positive cells on each slide was determined visually using a Nikon Labphoto-2 microscope. At least 100 cells were counted per slide and experiments were done in duplicate. Percentage inhibition is measured by counting the number of stress fiber positive cells in the presence of the test compound when compared to the number of stress fiber positive cells in the absence of the test compound.
Using the above protocols, all of the compounds as disclosed herein are determined to have Rho-kinase inhibitory activity.
The compounds of the invention can be made according to routine, conventional chemical methods, and/or as disclosed below, from starting materials which are either commercially available or produceable according to routine, conventional chemical methods. General methods for the preparation of the compounds are given below, and the preparation of representative compounds is specifically illustrated in the Examples.
When the following abbreviations are used herein, they have the following meaning:
General Methods of Preparation
A mixture of compounds. 1 and 2, and potassium acetate in THF/water is stirred at room temperature overnight. Water is added to the mixture resulting in the formation of a precipitate. The precipitate is washed with water, filtered, and dried under high vacuum to afford 3.
A mixture of compound 3, ethylene glycol dimethyl ether/water, Aryl boronic acid and sodium bicarbonate is degassed with argon for 15 minutes and Pd(dppf)Cl2 is added. The mixture is heated to reflux overnight. After cooling to rt CH2Cl2 and H2O are added to the mixture. The organic and aqueous layers are separated and the aqueous layer is extracted with CH2Cl2, and the combined organic layers are dried over anhydrous sodium sulfate. The organic solvent is removed under reduced pressure and the crude product is purified by silica gel chromatography of HPLC to afford compound 4.
A mixture of compound. 3 and a substituted amine or aniline, is heated to 140° C. for 2 hours. The mixture is cooled to room temperature and is treated with ether to form precipitate or is purified by silica gel column chromatography. Purification of precipitate: The precipitate is filtered, washed with ether several times, and is dried under high vacuum to provide product.
It is to be understood that the specific conditions selected from these General Methods A-C will depend on the particular structures of the starting materials chosen, in order to optimized the yield of the products desired.
Without further elaboration, it is believed that one skilled in the art can, using the preceding description, utilize the present invention to its fullest extent. The following preferred specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.
In the foregoing and in the following examples, all temperatures are set forth uncorrected in degrees Celsius; and, unless otherwise indicated, all parts and percentages are by weight.
The entire disclosure of all applications, patents and publications, cited above or below, including U.S. Provisional Application No. 60/277,974, filed Mar. 23, 2001 and U.S. Provisional Application No. 60/315,341, filed Aug. 29, 2001, are hereby incorporated by reference.
A solution of P(O)Cl3 (800 mL) and DMF (4 mL) stirred at room temperature for 20 min and is added to a flask containing benzoyleneurea (200 g). The mixture is heated to reflux overnight. The brown solution is cooled to 50° C., poured into cold water (0° C., 8000 mL) while stirring vigorously. The aqueous mixture is maintained at a temperature below 30° C. during the quench. The cold precipitate is filtered, washed with cold water (3×1200 mL) and dried under high vacuum at 40° C. to afford 174 g of Intermediate A (71%).
A mixture of 2,4-dichloroquinazoline (Intermediate A, 174 g, 0.874 mol), 5-aminoindazole (130 g, 0.98 mol), and potassium acetate (111.5 g, 1.14 mol) in THF/water (2 L/0.9 L) is stirred at room temperature overnight. Water (2 L) is added to the mixture resulting in the formation of a precipitate. The precipitate is washed with water, filtered, and dried under high vacuum to afford Intermediate B (241 g, 0.8 mol, 92%) as a gray powder.
A mixture of 2-N-5′-aminoindazole-4-chloroquinazoline (0.21 g), ethylene glycol dimethyl ether/water (50 mL/6 mL), 2,4-dichlorophenyl boronic acid (0.11 g) and sodium bicarbonate (0.18 g) is degassed with argon for 15 minutes and Pd(dppf)Cl2 (0.042 g) is added. The mixture is heated to reflux overnight. After cooling to rt CH2Cl2 (100 mL) and H2O (50 mL) were added to the mixture. The organic and aqueous layers were separated and the aqueous layer is extracted with CH2Cl2 (2×75 mL), and the combined organic layers were dried over anhydrous sodium sulfate. The organic solvent is removed under reduced pressure and the crude product is purified by silica gel chromatography to afford Example 1 (0.08 g). Rf=0.52 (CH2Cl2/MeOH=95/5). 1H NMR (CD3OD) δ 8.44 (1H, dd, J=2.7 Hz), 8.23 (1H, s), 8.01 (1H, s), 7.89-7.85 (2H, m), 7.84-7.78 (1H, m), 7.73-7.65 (2H, m), 7.58-7.53 (2H, m), 7.43 (1H, dd, J=1.2, 2.7 Hz).
Using an analogous procedure to that described for Example 1, Intermediate B (prepared in as described in Step 2) is reacted with the appropriate substituted boronic acid Ar1B(OH)2 to give the compounds of Examples 2-24 described in Table 1 below:
1) Rf=0.49 (CH2Cl2/MeOH=95/5). 1H NMR (CD3OD) δ 8.47 (2H, d, J=8.4 Hz), 8.23 (1H, s), 8.09-8.34 (2H, dd, J=8.0, 8.4 Hz), 7.89-7.83 (2H, m), 7.73-7.59 (3H, m), 7.71 (1H, d, J=8.4 Hz), 7.26-7.18 (1H, m), 2.63 (3H, s).
2) Rf=0.50 (CH2Cl2/MeOH, 95/5). 1H NMR (CD3OD) δ 8.41 (1H, s), 8.35-8.34 (2H, m), 8.20 (1H, d, J=3.0 Hz), 8.09 (1H, s), 7.88-7.82 (1H, m), 7.70-7.57 (3H, m), 7.46-7.43 (1H, d, J=9 Hz), 7.35 (2H, d, J=9 Hz)
3) Rf=0.53 (CH2Cl2/MeOH, 95/5). 1H NMR (CD3OD) δ 8.58 (2H, d, J=8.4 Hz), 8.36 (1H, d, J=8.5 Hz), 8.22 (1H, d, J=1 Hz), 8.06 (1H, d, J=1 Hz), 7.89-7.83 (3H, m), 7.71 (1H, d, J=8.4 Hz), 7.63-7.59 (3H, m)
4) Rf=0.53 (CH2Cl2/MeOH, 95/5). 1H NMR (DMSO) δ 13.20 (1H, s), 10.05 (1H, s), 8.57 (1H, d, J=10.0 Hz), 8.50 (1H, dd, J=11.0, 1.0 Hz), 8.46-8.31 (2H, m), 8.17 (1H, d, J=1.2 Hz). 8.10 (1H, s), 7.91-7.84 (2H, m), 7.77-7.74 (1H, m), 7.52 (1H, dd, J=9.0, 9.2 Hz), 7.35 (1H, J=8.4, 8.4 Hz)
5) Rf=0.47 (CH2Cl2/MeOH, 95/5). 1H NMR (CD3OD) δ 8.24 (1H, d, J=9 Hz), 8.20 (1H, s), 8.07 (1H, s), 8.04-7.98 (1H, m), 7.88-7.79 (2H, m), 7.69-7.61 (3H, m). 7.18-7.16 (1H, m), 6.86 (1H, d, J=8.1 Hz), 6.16 (2H, s)
6) Rf=0.53 (CH2Cl2/MeOH, 95/5). 1H NMR (CD3OD) δ 8.25-8.22 (1H, m), 8.06 (1H, s), 7.85-7.80 (1H, m), 7.60-7.44 (4H, m), 7.24 (1H, d, J=6.3 Hz), 7.16-7.12 (2H, m). 6.94 (1H, d, J=7.8 Hz), 6.66 (1H, d, J=8.1 Hz), 3.30 (3H, s).
7) Rf=0.48 (Hexane/EtOAc, 50/50). 1H NMR (CD3OD) δ 8.50 (1H, d, J=1.8 Hz), 8.36 (1H, d, J=9 Hz), 8.25 (1H, d, J=9.3 Hz), 8.19 (1H, d, J=2.1 Hz), 8.07 (1H, s), 7.86-7.78 (3H, m). 7.62-7.55 (3H, m)
8) Rf=0.50 (CH2Cl2/MeOH, 95/5). 1H NMR (DMSO) δ 9.99 (1H, s), 8.95 (1H, s), 8.56 (1H, d, J=8.4 Hz), 8.53 (1H, d, J=9.0 Hz), 8.35 (1H, d, J=1.5 Hz), 8.17 (1H, s). 8.00 (1H, d, J=8.1 Hz), 7.95-7.82 (3H, m), 7.68-7.54 (5H, m)
9) Rf=0.51 (Hexane/EtOAc, 3/2). 1H NMR (CD3OD) δ 8.33 (1H, s), 8.26 (1H, s), 8.18 (1H, s), 7.90-7.86 (2H, m), 7.68-7.45 (3H, m), 7.26-7.17 (1H, m), 6.87 (1H, s), 3.38 (6H, s), 3.34 (3H, s)
10) Rf=0.46 (Hexane/EtOAc, 2/1). 1H NMR (CD3OD) δ 8.43 (1H, d, J=9.8 Hz), 8.33-8.29 (1H, m), 8.14 (1H, s), 7.95 (1H, d, J=9.4 Hz), 7.89-7.86 (2H, m), 7.72-7.61 (4H, m), 7.55 (1H, d, J=1.0 Hz), 7.43-7.39 (1H, m), 7.38-7.34 (1H, m).
11) Rf=0.35 (Hexane/EtOAc, 2/1). 1H NMR (CD3OD) δ 8.34 (1H, d, J=8.4 Hz), 8.24-8.22 (2H, m), 8.09 (1H, s), 7.87-7.81 (4H, m), 7.60 (1H, d, J=8.7 Hz), 7.57-7.53 (1H, m), 7.54 (1H, dd, J=3, 2 Hz)
12) Rf=0.35 (Hexane/EtOAc, 2/1). 1H NMR (CD3OD) δ 8.38-8.34 (2H, m), 8.08 (1H, d, J=1 Hz), 7.96 (1H, dd, J=1.2, 2.7 Hz), 7.84-7.80 (3H, m), 7.60-7.53 (3H, m), 7.14 (1H, dd, J=3.9, 5.1 Hz)
13) Rf=0.49 (Hexane/EtOAc, 2/1). 1H NMR (CD3OD) δ 8.60 (1H, d, J=8.4 Hz), 8.16-8.15 (2H, m), 8.10 (1H, d, J=7.5 Hz), 8.02 (1H, d, J=7.8 Hz), 7.87 (1H, t, J=7.8 Hz), 7.82-7.75 (3H, m), 7.72 (1H, t, J=9.0 Hz), 7.51 (1H, t, J=7.8 Hz), 7.25 (1H, dd, J=2.4, 7.2 Hz), 3.80 (3H, s)
14) Rf=0.51 (Hexane/EtOAc, 2/1). 1H NMR (CD3OD) δ 8.62 (1H, d, J=8.8 Hz), 8.16-8.14 (2H, m), 8.09 (1H, dd, J=1.2, 7.5 Hz), 8.32 (1H, d, J=8.4 Hz), 7.87 (1H, t, J=7.8 Hz), 7.82-7.72 (5H, m), 7.51 (1H, t, J=8.4 Hz), 7.24 (1H, dd, J=3.6, 4.8 Hz), 3.80 (3H, s)
15) Rf=0.52 (CH2Cl2/MeOH, 95/5). 1H NMR (CD3OD) δ 8.36 (1H, d, J=7.5 Hz), 8.30 (1H, d, J=6.9 Hz), 8.24 (1H, d, J=2.4 Hz), 8.09 (1H, s), 7.87-7.84 (3H, m), 7.63-7.55 (4H, m), 6.97 (1H, d, J=9.0 Hz), 4.10 (2H, q, J=6.9 Hz), 1.41 (3H, t, J=6.9 Hz)
16) Rf=0.43 (CH2Cl2/MeOH, 95/5). 1H NMR (CD3OD) δ 8.54 (1H, d, J=8.4 Hz), 8.11 (1H, s), 8.07 (1H, t, J=10.5 Hz), 8.01 (1H, d, J=1.0 Hz), 77.88-7.82 (2H, m), 7.65-7.63 (2H, m), 2.57 (3H, S), 2.29 (3H, s)
17) Rf=0.43 (Hexane/EtOAc, 2/1). 1H NMR (CD3OD) δ 8.46 (2H, d, J=9.6 Hz), 8.39 (1H, dd, J=8.6, 0.6 Hz), 8.26 (1H, dd, J=2.1, 1.0 Hz), 8.10 (1H, d, J=1.5 Hz), 7.91-7.83 (3H, m), 7.74-7.59 (6H, m), 7.44 (2H, dd, J=6.9, 8.4 Hz), 7.35 (1H, d, J=7.5 Hz)
18) Rf=0.43 (Hexane/EtOAc, 2/1). 1H NMR (CD3OD) δ 8.22 (1H, d, J=8.2 Hz), 8.19-8.17 (2H, m), 8.09 (1H, d, J=9.3 Hz), 7.88-7.81 (3H, m), 7.71-7.59 (3H, m), 6.80 (2H, d, J=7.2 Hz), 3.06 (6H s)
19) Rf=0.42 (Hexane/EtOAc, 1/3). 1H NMR (DMSO) δ 13.09 (1H, s), 10.00 (1H, s), 8.58 (1H, d, J=8.1 Hz), 8.36 (1H, s), 8.18 (2H, s), 8.00-7.94 (2H, m), 7.87-7.82 (3H, m), 7.65-7.61 (2H, m), 7.39 (2H, t, J=4.5 Hz)
20) Rf=0.46 (CH2 Cl2/MeOH, 95/5). 1H NMR (DMSO) δ 13.09 (1H, s), 10.21 (1H, s), 10.00 (1H, s), 8.58 (1H, d, J=8.2 Hz), 8.24-8.16 (3H, m), 8.18 (1H, s), 7.91-7.78 (3H, m), 7.68-7.48 (2H, m), 7.86 (2H, d, J=7.8 Hz)
21) Rf=0.50 (EtOAc/Hex, 1/1): Retention time (HPLC): Rt=5.73. 1H NMR (CD3OD): δ 8.7 (d, J=8.1 Hz, 1H), 8.3-8.4 (dd, 2H), 8.2 (d, J=1.8 Hz, 1H), 8.0-8.2 (m, 4H), 7.8-7.9 (m, 2H), 7.7 (q, J=3.3 Hz, 2H), 7.5-7.6 (m, 3H). HPLC/MS: (M+H)+ m/z 428.5.
22) HPLC/MS: (M+H)+m/z 432.2. RT (min) LC/MS: 2.77. 1H NMR (CD3)2SO): δ 7.46 (m, 3H); 7.63 (m, 5H); 7.83 (dd, J=1.9, 9.0 Hz, 1H); 7.87 (m, 2H); 8.13 (br s, 1H); 8.17 (dd, J=1.6, 12.5 Hz, 1H); 8.22 (d, J=1.9 Hz, 1H); 8.30 (dd, J=1.6, 8.0 Hz, 1H); 8.58 (br d, J=8.5 Hz, 1H); 10.04 (s, 1H); 13.13 (br s, 1H).
To a solution of dimethylamine (excess) in THF is added a substituted benzoyl chloride dropwise at 0° C. The reaction mixture is stirred at room temperature for 2 hours. After removal of the solvent under reduced pressure the residue is dissolved EtOAc, and washed with water (3×). The organic layer is concentrated in vacuo and the crude product is either used directly or purified by silica gel chromatography (gradient from 10% to 50% ethyl acetate/hexane).
A solution of a substituted N,N-dimethylbenzamide (1.17 g, 7.9 mmol) and POCl3 (3.0 g, 19.7 mmol) is stirred at 0° C. for 30 minutes. To this mixture is added 5-chloro-2-amino-benzonitrile (1.0 g, 6.6 mmol) and CH2Cl2 (5.0 ml). The reaction mixture is stirred at 40° C. for 18 hours. The mixture is poured into ice water, basified to pH 9 with NaHCO3, and extracted with CH2Cl2. The organic layer is dried over MgSO4 and concentrated in vacuo. The crude product is purified by silica gel column (ethyl acetate/hexane, 10/90). Thus is obtained the Intermediate Cl, (R═H) (0.45 g, 25%) as pale yellow powder. HPLC/MS: (M+H)+275.2 m/z. Retention time (HPLC/MS)=3.97 min.
Using the same procedure described above for Intermediate C1 and substituting the appropriate benzamide intermediate starting material, Intermediates C2 to C6 were similarly prepared and are summarized in Table 3:
A mixture of 4,7-dichloro-2-phenylquinazoline (20 mg, 0.05 mmol) and 5-aminoindazole (7.5 mg, 0.06 mmol) in butanol (2.0 ml) is heated to 100° C. overnight. After removal of solvent in vacuo the crude product is purified by silica gel column chromatography (gradient from 20% to 80% ethyl acetate/hexane) to afford Example 25 (15.2 mg). HPLC/MS: (M+H)+372.4 m/z. Retention time (HPLC/MS)=2.53 min.
Using the method described for Example 25 and using the appropriate substituted 4-chloro-2-arylquinazoline and 5-aminoindazole as starting materials, Examples 26-32 were similarly prepared and are summarized in Table 4 below:
Step 1: To a solution of anthranilonitrile (7.58 mmol) in dry pyridine (30 mL) is added 2-quinoxaloyl chloride (9.11 mmol, 1.2 equivalent). The reaction mixture stirred at room temperature overnight and sodium hydroxide solution (2%, 50 mL) is added. The mixture is cooled and stirred for 30 min. The resulting white solid is collected by filtration, washed with brine and cold ether. A white solid product is obtained (1.51 g, 73%). HPLC/MS: (M+H)+=275, RT (HPLC/MS)=3.0 min.
Step 2: The amide prepared in Step 1 (9.5 mmol, 1 equivalent) is suspended in dioxane (10 mL). NaOH solution (20%, 60 mL) and hydrogen peroxide solution (30%, 30 mL) is added in three portions. A vigorous release of gas is observed. The reaction mixture continued to stir and is cooled when necessary until the evolution of gas ceased. The reaction is brought to 120° C. (oil bath) and stirred overnight at this temperature. The reaction is neutralized with concentrated HCl to pH=7. A precipitate formed and is collected on a funnel, washed with water and dried in vacuo. A yellow solid is obtained and used in the next step without further purification. HPLC/MS: (M+H)+=275, RT (HPLC/MS)=3.28.
Step 3: The quinazoline (10.9 mmol) is suspended in phosphorous oxychloride (214.6 mmol) containing PCl5 (10.9 mmol) and stirred at 115° C. for 18 h. The resulting yellow solution is poured into 300 mL of ice and stirred. A gray precipitate formed and filtered and washed with cold water. The product is used in the next step without further purification. HPLC/MS: (M+H)+=293, RT (LC-MS)=3.40.
Step 4: A mixture of 4-chloroquinazoline, potassium acetate (14.25 mmol), and 5-aminoindazole (10.96 mmol) in THF/H2O (70 mL/25 mL) is stirred at room temperature for 17 h. The resulting solid is collected by filtration and purified by silica gel column chromatography (gradient, 5-10% MeOH/CH2Cl2) to afford the product (1.19 g, 32%, 3 steps) as yellow powder. HPLC/MS: (M+H)+=390, RT (LC-MS)=2.41.
Step 1: To a solution of 6-fluoro-2-amino-benzonitrile (2 mmol, 1 equivalent.) in pyridine (3 mL) and CH2Cl2 (1 mL) containing N-dimethylaminopyridine (3 mg) is added 2-toluoyl chloride (316 mL, 1.2 equivalent). The reaction mixture is shaken at room temperature for 48 h and poured into cold water (3 mL) and shaken for 1 h. The resulting solid is filtered and washed with water to afford a white solid (90%). The LC-MS is consistent with the desired compound.
Step 2: The product is suspended in aqueous NaOH (20%, 2 mL) and dioxane (1 mL). Hydrogen peroxide (30%, 1 mL) is added in potions to avoid vigorous formation of gas. The reaction is shaken at 85° C. for 20 h and then is neutralized with acetic acid to pH-7. The resulting precipitate is collected by filtration, washed with water and ether, and dried over P2O5 for two days. The product is suspended in P(O)Cl3 (4 mL) and shaken at 90° C. overnight. The POCl3 is removed in vacuo and co-evaporated with toluene. The resulting yellow solid residue is dried in vacuo overnight and used in the next step without further purification
Step 3: The product (assumed to be 2 mmol), 5-aminoindazole (3 mmol, 1.5 equivalent), and potassium carbonate (2 mmol) were suspended in DMF (5 mL) containing and shaken at 90° C. for 24 h. The reaction suspension is filtered and the filtrate is purified by HPLC, under the following conditions:
Column: YMC C18 Pro, 20×150 m/m; Gradient: A=H2O, 0.1% TFA, B=CH3CN, 0.1% TFA; Gradient over 10 min, flow: 30 mL/min. A pale yellow solid product is obtained. (M+H)+=370, RT (LC-MS)=2.19 min.
Using the methods described above for Examples 34 and substituting the appropriate starting materials, the compounds listed in Table 5 were also synthesized.
2-Chloro-N-(1H-indazol-5-yl)-6,7-dimethoxy-4-quinazolinamine (prepared from 3,4-dimethoxybenzoylurea by the method described for Example 1, steps 1 and 2) (0.1 mmol) is suspended in toluene (1 mL), n-BuOH (0.5 mL), and Na2CO3 (0.5 mL, 2M aqueous). The reaction mixture is degassed for 20 min with argon followed by the addition of 4-fluorophenyl boronic acid (0.4 mmol) and Pd-catalyst (0.05 mmol) The mixture is heated to reflux and stirred for 72 h. The solvent is removed in vacuo and the residue is purified by preparative silica gel TLC (5% MeOH/CH2Cl2) to obtain a yellow solid :HPLC/MS: (M+H)+=416, RT (HPLC/MS)=2.96.
Using the method described above for Example 75 and substituting the appropriate starting materials Examples 76-80 similarly prepared and are shown in Table 6.
A suspension of 2-chloro-N-(1H-indazol-5-yl)-6,7-dimethoxy-4-quinazolinamine (0.1 mmol) and 3-fluoroaniline (0.3 mmol) in n-butanol (1 mL) is shaken at 90° C. for 72 h. The solvent is evaporated off and the residue is purified by HPLC to afford pure product. (M+H)+=431, RT (LC-MS)=2.94.
Using the method described above for Example 81, and substituting the appropriate starting materials, Examples 82-107 were similarly prepared and are summarized below in Table 7.
A mixture of 2-chloro-N-(1H-indazol-5-yl)-4-quinazolinamine (30 mg, 0.1 mmol) and a substituted aniline (2 mmol) is heated to 140° C. for 2 hrs. The mixture is cooled to it and treated with ether to form precipitate which is washed with ether several times and dried under high vacuum to provide product. Alternatively, the product is purified by silica gel column chromatography by dissolving the solid in dichloromethane and loaded on to a column which is eluted (hexanes/ethyl acetate, gradient) to give desired product.
Using this method and substituting the appropriate aniline starting materials, Examples 108-129 were prepared and are summarized in Table 8 below:
According to the method of Suesse, M.; Adler, F.; and Johne, S. (Helv. Chim. Acta 1986, 69 1017), a mixture of 2-aminobenzamide (20 g, 147 mmol) and diethyl oxalate (39.9 mL, 42.9 g, 294 mmol) is warmed to 170-180° C. for 6 h. The mixture is cooled to rt and diluted with EtOH. The resultant precipitate is filtered and washed thoroughly with EtOH to afford a crude solid, which could be further purified by recrystallization from EtOH (21.1 g, 66%).
A mixture of material from Step 1 (1.0 g, 4.6 mmol), thionyl chloride (4.0 mL, 6.5 g, 55 mmol), and N,N-dimethylformamide (5 drops) in chloroform (10 mL) is heated to for 4 h. The mixture cooled to rt and the volatiles were removed under vacuum. The resultant crude solid is dried under vacuum overnight to afford the desired intermediate (1 g, 92%) which is used in the next step without additional purification.
A mixture of compound from Step 2 (1 g, 4.23 mmol), 5-aminoindazole (0.560 mg, 4.23 mmol), HCl (15 mL, 0.12 N, aqueous) and n-BuOH (4.3 mL) is warmed to 100° C. for 4 h. The mixture cooled to rt and the resultant precipitate is removed by filtration. The solid is washed thoroughly with EtOAc and CH2Cl2, and is dried under vacuum overnight to afford the product as an orange solid (1.21 g, 77%). mp (° C.): 215-219; TLC Rf=0.23 (90/10, CH2Cl2/MeOH)
To a suspension of the Step 3 amine hydrochloride salt (0.11 g, 2.03 mmol) in toluene (5 mL) at it is added the trimethylaluminum (1.00 mL, 2.0 M in heptanes, 2.0 mmol) dropwise. The mixture stirred until gas evolution ceased, approximately 1 hour. The newly formed solution of trimethylaluminum and ammonium chloride is added dropwise to a solution of product from Step 3 (0.15 g, 0.41 mmol) in toluene (5 mL) at rt. The reaction mixture is heated to reflux, and stirred for 5 h. The reaction is cooled to rt, and quenched slowly with 5% aqueous HCl (2 mL). The biphasic mixture is filtered through Extrelut, and the filtering aid is washed thoroughly with EtOAc. The combined organic ishes and filtrates were concentrated, and the crude product is purified by reversed phase HPLC to afford Example 130 (0.032 g, 26%). mp. (° C.): 300; TLC Rf=0.05 (90/10, CH2Cl2/MeOH) 0.05.
By using the above method and substituting the appropriate starting materials, Examples 131-138 were synthesized in analogous manner and are summarized in Table 9.
To a solution of anthranilamide (1.6 g, 11.6 mmol), pyridine (1.1 mL, 13.9 mmol) and CHCl3 (55 mL) is added acetyl chloride (91 pt, 12.7 mmol), dropwise. The reaction stirred at room temperature for 2 h. The volatiles were removed by evaporation and the residue is partitioned between EtOAc and 1 N sodium carbonate. The resulting precipitate is collected by filtration. The layers of the filtrate were separated and the organic phase is washed with 1 N HCl, dried (MgSO4), and evaporated. The filtered solid product and the evaporated solid were combined and dried under vacuum to afford the desired intermediate. (1.1 g, 6.2 mmol; 54% yield); Rf=0.47 (EtOAc/hexanes, 50/50); 1H NMR (DMSO-d6) 11.55 (s, 1H), 8.39 (d, J=8.2, 1H), 8.22 (s, 1H), 7.74 (m, 2H), 7.07 (m, 1H), 7.07 (m, 1H), 2.07 (s, 1H); ES MS (M+H)+=179.
To a mixture of diamide from Step 1 (890 mg, 5.0 mmol) in EtOH (30 mL) is added 10 N NaOH (1.49 mL, 14.9 mmol). The reaction is heated to reflux for 4 h, cooled to room temperature and the volatiles were evaporated. The aqueous mixture is acidified to pH=5 with concentrated HCl. The mixture is evaporated until a precipitate formed. The solids were collected by filtration, washed with hexanes and dried under vacuum to afford the desired intermediate (564 mg, 3.5 mmol; 71% yield); Rf=0.10 (EtOAc/hexanes, 50/50); 1H NMR (DMSO-d6) 8.11 (dd, J=1.0, 7.8, 1H), 7.89 (m, 1H), 7.74 (d, J=8.1, 1H), 7.58 (m, 1H), 2.53 (s, 3H); ES MS (M+H)+=161.
A thoroughly homogenized mixture of 5-aminoindazole (831 mg, 6.2 mmol), phosphorous pentoxide (886 mg, 6.2 mmol), and triethylamine hydrochloride (859 mg, 6.2 mmol) is heated at 200° C. to obtain a melt. After 1 h the hydroxyquinazoline from Step 2 (250 mg, 1.6 mmol) is added in one portion and the mixture is kept at 200° C. for 16 h. The mixture is cooled to 135° C., 9:1 H2O-MeOH (10 mL) is added, and mixture is sonicated. The mixture is decanted, adjusted to pH=9 with concentrated ammonium hydroxide, and concentrated under vacuum. The residue is purified by flash chromatography (CH2Cl2-MeOH, 100/0-90/10 gradient). The fractions containing product were combined and the volatiles were removed by evaporation. The residue is partitioned between 1 N NaOH and EtOAc. The organic layer is removed, dried (MgSO4), and evaporated. The residue is further purified by preparative TLC (CH2Cl2-MeOH, 95/5-90/10 gradient) and dried under vacuum to afford Example 139 (17 mg, 0.062 mmol, 4% yield); Rf=0.45 (EtOAc/hexanes, 90/10); mp=282-288° C.; ES MS (M+H)÷=276. 7 Hz), 1.40 (3H, t, J=5.7 Hz).
The following process can be used to prepare the single compound
A suspension of magnesium (0.968 g, 3.98 mmol) and a few crystals of iodine in anhyd THF (200 mL) were treated with dropwise addition of 10 mL of a solution of 4-bromo-2-fluorobiphenyl (10.0 g, 3.98 mmol) in THF (100 mL). The mixture was heated to gentle reflux and a reaction ensued. At that time, the remaining solution of 4-bromo-2-fluorobiphenyl was added dropwise to the flask over a 3-minute period. The contents were then stirred at reflux under argon until no magnesium consumption was observed. The reaction mixture was subsequently cooled to −10° C. and treated with dry ice (˜70 g). The reaction mixture was quenched with 20% aqueous hydrochloric acid (50 mL), and the layers were separated. The aqueous phase was extracted with ethyl acetate (2×20 mL), and the combined organic layer was washed with brine (30 mL), dried over anhyd sodium sulfate and concentrated to about ⅓ of its original volume. The contents were treated with hexane (200 mL), and the precipitate was filtered and dried under high vacuum to afford 3-fluoro-4-phenylbenzoic acid (6.37 g, 74%) as a white, crystalline solid. 1H-NMR (DMSO-db): δ 7.48 (m, 3H); 7.59 (m, 2H); 7.66 (dd, J=8.1, 8.1 Hz, 1H); 7.76 (dd, J=1.5, 11.6 Hz, 1H); 7.85 (dd, J=1.5, 8.1 Hz, 1H); 13.30 (br s, 1H). Anal. Calcd for C13H9FO2: C, 72.22; H, 4.20; F, 8.79. Found: C, 71.95; H, 4.11; F, 9.07.
A suspension of the product of step 1 (0.5 g, 2.31 mmol) in oxalyl chloride (5 mL) was treated with one drop of DMF and the mixture was heated to 60° C. for 45 min. The resulting, clear-yellow solution was concentrated to a yellow solid, which was dried under high vacuum for 60 min. The solid and anthranilamide (0.314 g, 2.31 mmol) were suspended in dry toluene (5 mL), treated with diisopropylethylamine (0.5 ml, 0.371 g, 2.87 mmol) and the contents were stirred at room temperature for 2 h. at which time TLC (silica gel 60, 10% methanol/dichloromethane, UV detection) analysis suggested complete reaction. The mixture was filtered, and the off-white solid was dissolved in ethyl acetate (50 mL). The organics were washed with brine (25 mL), 0.1 N aqueous hydrochloric acid (25 mL), and again with brine (25 mL). The organic layer was dried over anhyd sodium sulfate, concentrated and dried under high vacuum for 4 h to afford the product (0.59 g, 1.76 mmol, 76%) as an off-white solid. 1H-NMR (DMSO-d6): δ 7.22 (ddd, J=1.2, 7.4, 7.8 Hz, 1H); 7.52 (m, 6H); 7.78 (m, 3H); 7.89 (m, 1H); 7.89, 8.47 (br s, 2H); 8.69 (dd, J=1.2, 8.3 Hz, 1H); 13.12 (s, 1H). Anal. Calcd for C20H15N2FO2: C, 71.85; H, 4.52; N, 8.38. Found: C, 71.67; H, 4.47; N, 8.35. Mass spectrum (HPLC/ES, flow injection): m/e=335 (M+1).
Method A
A suspension of the product of step 2 (0.5 g, 2.31 mmol) in oxalyl chloride (5 mL) was treated with one drop of DMF and the mixture was heated to 60° C. for 60 min. The resulting clear yellow solution was concentrated to a yellow solid, which was dried under high vacuum for 2 h. This solid and anthranilamide (0.314 g, 2.31 mmol) were dissolved in dry THF (5 mL), treated with diisopropylethylamine (0.5 ml, 0.371 g, 2.87 mmol) and the contents were stirred at room temperature for 90 min, at which time TLC (silica gel 60, 5% methanol/dichloromethane, UV detection) analysis suggested complete reaction. The mixture was treated with aqueous 1.0 N sodium hydroxide (10.0 mL, 10.0 mmol). The contents were heated to 50° C. (complete dissolution occurred when the internal temperature reached 44° C.) for 90 min and the organic solvent was removed by rotary evaporation. The aqueous suspension was treated with dropwise addition of aqueous 2.0 N hydrochloric acid (about 5 mL) until the pH was adjusted to about 2. The precipitate was filtered and the cake was washed with water (4×30 mL) and dried under high vacuum at 40° C. for 18 h to provide the product (0.67 g, 2.12 mmol, 92%) as a white powder. 1H-NMR (DMSO-d6): δ 7.52 (m, 4H); 7.64 (m, 2H); 7.75 (m, 2H); 7.86 (ddd, J=1.4, 6.9, 8.0 Hz, 1H); 8.16 (m, 3H); 12.63 (br s, 1H). Anal. Calcd for C20H13N2FO: C, 75.94; H, 4.14; N, 8.86. Found: C, 75.66; H, 4.29; N, 8.77. Mass spectrum (HPLC/ES): m/e=317 (M+1).
Method B.
A suspension of the product of step 1 (0.5 g, 2.31 mmol) in oxalyl chloride (5 mL) was treated with one drop of DMF and the mixture was heated to 60° C. for 60 min. The resulting clear yellow solution was concentrated to a yellow solid, which was dried under high vacuum for 60 min. This solid and anthranilamide (0.314 g, 2.31 mmol) were suspended in dry toluene (5 mL), treated with diisopropylethylamine (0.5 ml, 0.371 g, 2.87 mmol) and the contents were stirred at room temperature for 2 h, at which time TLC (silica gel 60, 10% methanol/dichloromethane, UV detection) analysis suggested complete reaction. The mixture was filtered and dried under high vacuum for 2 h. The off-white solid was then dissolved in methanol (10 mL) and THF (5 mL), and the solution was treated with aqueous 1.0 N sodium hydroxide (10.0 mL, 10.0 mmol). The contents were heated to 45° C. for 2 h and the organic solvents were removed by rotary evaporation. The aqueous suspension was treated with dropwise addition of aqueous 2.0 N hydrochloric acid until the pH was adjusted to about 2 (5 mL). The precipitate was filtered and the cake was washed with water (4×30 mL) and dried under high vacuum at 40° C. for 3 h to provide product (0.66 g, 2.09 mmol, 90%) as a white powder. 1H-NMR (DMSO-d6): δ 7.52 (m, 4H, aromatic); 7.64 (m, 2H, aromatic); 7.75 (m, 2H); 7.86 (ddd, J=1.4, 6.9, 8.0 Hz, 1H, aromatic); 8.16 (m, 3H, aromatic); 12.63 (br s, 1H, —NH). Anal. Calcd for C20H13N2FO.0.20H2O: C, 75.08; H, 4.22; N, 8.76. Found: C, 75.08; H, 4.03; N, 8.67. Mass spectrum (HPLC/ES): m/e=317 (M+1).
A solution of phosphorous oxychloride (3.0 mL) and anhyd DMF (2 mL) was stirred for 10 min before it was added to a flask containing the product of step 3 (0.300 g, 0.948 mmol). The resulting suspension was heated to gentle reflux under argon for 12 h. The dark solution was then cooled to 70° C. and slowly added to vigorously-stirred water (100 mL) at 0° C. A solid precipitated, which was stirred for 10 min and filtered. The cake was washed with water (2×25 mL) and dried under high vacuum at 35° C. for 2 h to provide product (0.285 g, 0.851 mmol, 90%) as a yellow solid. Part of this solid (0.125 g) was passed through a short plug of silica gel using 20% dichloromethane/hexane as eluant to afford the title compound (0.09 g) as white needles. 1H-NMR (DMSO-d6): δ 7.47 (m, 1H); 7.54 (m, 2H); 7.65 (m, 2H); 7.76 (dd, J=8.4, 8.4 Hz, 1H); 7.87 (ddd, J=2.9, 5.3, 8.3 Hz, 1H); 8.15 (m, 2H); 8.26 (m, 1H); 8.28 (m, 1H); 8.38 (dd, J=1.9, 8.4 Hz, 1H). Anal. Calcd for C20H12N2ClF: C, 71.75; H, 3.61; N, 8.37; Cl, 10.59. Found: C, 71.54; H, 3.48; N, 8.29; Cl, 10.61. Mass spectrum (HPLC/ES): m/e=335 (M+1). TLC (silica gel 60, 40% dichloromethane/hexane, UV detection): one spot, Rf=0.50.
To a suspension of the product of step 4 (1.00 g, 2.99 mmol) and 5-aminoindazole (0.44 g, 3.29 mmol) in ethylene glycol dimethyl ether(DME, 10 mL) was added a solution of potassium acetate (0.44 g, 4.48 mmol) in water (2 mL). The contents were allowed to reflux for 16 h and then cooled to room temperature. The mixture was poured into water (200 mL) and the precipitate was filtered, washed with water (2×50 mL) and air-dried for 60 min. The solid was dissolved in THF (30 mL), and the solution was slowly poured into hexane (500 mL). The resulting precipitate was filtered and dried under high vacuum at 60° C. for 18 h to afford the product (1.02 g, 2.36 mmol, 79%) as a yellow solid. 1H-NMR (DMSO-d6): δ 7.46 (m, 3H); 7.63 (m, 5H); 7.83 (dd, J=1.9, 9.0 Hz, 1H); 7.87 (m, 2H); 8.13 (br s, 1H); 8.17 (dd, J=1.6, 12.5 Hz, 1H); 8.22 (d, J=1.9 Hz, 1H); 8.30 (dd, J=1.6, 8.0 Hz, 1H); 8.58 (br d, J=8.5 Hz, 1H); 10.04 (s, 1H, —NH); 13.13 (br s, 1H). Mass spectrum (HPLC/ES): m/e=432 (M+1).
In order to prepare the p-toluene sulfonic acid (tosylate) salt, a suspension of the product (0.60 g, 1.39 mmol) in anhyd ethanol (12 mL) was treated with a solution of p-toluenesulfonic acid monohydrate (0.39 g, 2.09 mmol) in ethanol (8.5 mL) in one portion. The contents were stirred at 40° C. for 60 min and the precipitate was filtered. The cake was washed with ethanol (3×15 mL) and dried under high vacuum at 40° C. for 18 h to give the tosylate salt (0.71 g, 85%) as a pale-orange, crystalline solid. 1H-NMR (DMSO-do): δ 2.27 (s, 3H); 7.09, 7.47 (AA′BB′ quartet, J=8.6 Hz, 4H); 7.48 (m, 2H); 7.52 (m, 2H); 7.62 (m, 2H); 7.73 (m, 2H); 7.84 (m, 2H); 8.10 (m, 5H); 8.20 (s, 1H); 8.74 (br d, J=8.4 Hz, 1H); 11.50 (br s, 1H). Anal. Calcd for C27H18N5F.CH3C6H4SO3H: C, 67.65; H, 4.34; N, 11.60.
Found: C, 67.35; II, 4.46; N, 11.49. Mass spectrum (HPLC/ES): m/e=432 (M+1).
To a solution of 2-fluorophenyl ethyl ketone (4.41 g) in H2SO4 (10 mL) at 0° C. is added a mixture of NaNO3 (2.72 g) and H2SO4 (20 mL) dropwise to maintain the temperature. The reaction mixture warmed to room temperature slowly and stirred for 1 hour. The reaction mixture is poured over ice/water. The organic layer is washed with ice water (3×100 mL). The organic layer is dried over Na2SO4, filtered and evaporated under the reduced pressure. The crude product is purified by silica gel column chromatography (Hex/EtOAc, 4:1, Rf=0.77) to afford pure product nitro ketone 1.83 g (34%): 1H NMR (CDCl3) δ 8.73 (1H, dd, J=2.4, 4.8 Hz), 8.36-8.33 (1H, m), 7.29 (1H, t, J=6.9 Hz), 3.00 (2H, q, J=2.7 Hz), 1.20 (3H, t, J=5.4 Hz).
A solution of the compound prepared in step 1 (1.85 g, 9.34 mmol) hydrazine (0.33 mL, 10.3 mmol) in ethylene glycol (50 mL) is heated to 165° C. overnight. The reaction mixture cooled to room temperature and is extracted with EtOAc (3×150 mL). The combined organic layers were washed with H2O (2×50 mL), and dried over Na2SO4. The solvent is removed under the reduced pressure and the crude product is purified by silica gel column chromatography (Hex/EtOAc, 2:1, Rf=0.45) to afford the nitroindazole, 0.89 g (50%).
1H NMR (CDCl3) δ 8.60 (1H, s), 8.16 (1H, dd, J=1.5, 6.9 Hz), 7.38 (1H, d, J=6.9 Hz). 2.95 (2H, t, J=5.7 Hz), 1.33 (3H, t, J=5.7 Hz).
To a dry flask, purged with Argon, is added Pd/C followed by MeOH (20 mL). The nitro indazole of Step 2 is then added (0.89 g,) and the reaction is then charged with H2 (1 atm). The reaction mixture is stirred for 4 h and then filtered through a Celite® plug. The solvent is evaporated under reduced pressure to give a yellow crude product. Purification of the crude product by silica gel column chromatography (Hex/EtOAc, 2:1-1:2) afforded pure product, 0.68 g (91%): 1H NMR (CD3OD) δ 7.16 (1H, d, J=6.6 Hz), 6.90 (1H, d, J=0.6 Hz), 6.85 (1H, dd, J=12.6, 1.5 Hz). 2.80 (2H, t, J=5.7 Hz), 1.23 (3H, t, J=5.7 Hz).
Reaction of the aminoindazole of Step 3 with 2,4-dichlorquinazoline in a manner analogous to Example 1, Step 2 provided the desired Intermediate D which is used in the following steps without further purification.
By following a procedure analogous to Example 1, Step 3, and using intermediate D and the 4-methoxoyphenyl boronic acid as starting material, the product is prepared and characterized: 1H NMR (CD3OD, δ ppm) 8.58 (1H, d, J=6.3 Hz), 8.44-8.39 (3H, m), 7.83-7.81 (2H, m). 7.75 (1H, dd, J=1.5, 6.6 Hz), 7.56-7.53 (2H, m), 7.02 (2H, d, J=5.1 Hz), 3.79 (3H, s), 2.79 (2H, q, J=5.7 Hz), 1.20 (3H, t, J=5.7 Hz).
The preceding examples can be repeated with similar success by substituting the generically or specifically described reactants and/or operating conditions of this invention for those used in the preceding examples. By so doing the following compounds are also prepared:
This application is a continuation of U.S. application Ser. No. 10/103,566 filed Mar. 22, 2002 which claims the benefit of the filing date of U.S. Provisional Application No. 60/277,974, filed Mar. 23, 2001 and U.S. Provisional Application No. 60/315,341, filed Aug. 29, 2001.
| Number | Date | Country | |
|---|---|---|---|
| 60277974 | Mar 2001 | US | |
| 60315341 | Aug 2001 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 11354977 | Feb 2006 | US |
| Child | 12698386 | US | |
| Parent | 10103566 | Mar 2002 | US |
| Child | 11354977 | US |
