Patent Application
20180119156
The invention relates to the fields of cell biology, molecular genetics and genetic engineering. More particularly, the invention relates to the art of inducer-specific gene expression including materials, methods, systems and kits for performance of inducible gene expression.
Gene expression may be regulated by modulating the rate of transcription of DNA to RNAS (usually mRNA), or translation of mRNA into a polypeptide. Often, genes have a promoter which controls expression of a gene operably linked to that promoter region. Such promoters may be inducible in their activity by an inducer molecule, allowing for transcription of these genes to be turned on, or off, in response to the presence of inducer molecules. Recently, RNA-based gene control elements called riboswitches have attracted attention. Man-made riboswitches have been made and used.
Riboswitches are mRNA-based regulatory elements which allow for a ligand-dependent control of gene expression. A riboswitch comprises an aptamer which binds the inducer molecule (ligand). This ligand binding results in a structural change in the mRNA riboswitch which in turn may increase or decrease expression of the corresponding gene. Hence, riboswitches regulate gene expression at the translational level. For example there is the theophylline-responsive ON riboswitch for the csrA (carbon storage regulator) gene of Escherichia coli. This permits some control of cellular auto-aggregation and motility of the resulting E. coli switch-csrA mutant organism.
A number of small-molecule inducible expression systems have been developed in bacteria. These include lactose (lac), tetracycline (tet) and arabinose (ara) operons. These have been used for recombinant protein production, e.g. biopharmaceuticals and industrial biocatalysts.
Inducible expression systems are used in synthetic biology. For example, to control genetic circuits acting as sensors, as switches or as oscillators. The expression of novel metabolic pathways can be placed under control of inducible promoters, e.g. for fine chemicals, natural products—including drug precursors and fatty acids—or for biofuels. There is even the development of organisms induced by specific organic pollutants for use in bioremediation.
A review of current knowledge of engineered riboswitches and their application in gene expression is provided by Groher F. & Suess B. (2014) “Synthetic riboswitches—A tool comes of age.”Biochimica et Biophysica Acta 1839: 964-973.
Natural riboswitches are typically located in the 5′-untranslated region (UTR) of a mRNA, controlling the translation of the downstream coding sequence. Inspired by these naturally occurring cases, several synthetic riboswitches have been developed which harness the ability of riboswitches to regulate gene expression in response to exogenously applied stimuli. Again, they reside in the 5′ UTR of a reporter gene, they are induced by ligand-dependent structural rearrangements and they block translation of the reporter transcript either by masking the Shine-Dalgarno sequence or by nucleolytic cleavage by the riboswitch/ribozyme (Suess et al., 2004 Nucleic Acids Res 32:1610-1614; Ogawa and Maeda, 2007 Bioorg Med Chem Lett 17:3156-3160; Topp and Gallivan 2008 RNA 14:2498-2503; Mandal and Breaker 2004 Nat Rev Mol Cell Biol 5:451-483).
Building on the results of previous studies showing that regions of secondary structure in mRNA 5′-UTRs could cause substantial reductions in expression in prokaryotic and eukaryotic cells (Panaskeva et al. 1998 PNAS 95:951-956; Stripecke et al. 1994 Mol. Cell. Biol. 14:5898-5909; De Smit and Van Duin 1990 PNAS 87:7668-7672) ligand-inducible gene expression was accomplished in yeast by introduction of a small-molecule binding RNA into the 5′-UTR of a gene (Werstuck and Green, 1998 Science 282:296-298). This concept was extended to a variety of organisms by generating synthetic riboswitches which were responsive to theophylline (Desai and Gallivan 2004 J. Am. Chem. Soc. 126:13247-13254; Suess et al. 2004 Nucleic Acids Res. 32:1610-1614; Thompson et al. 2002 BMC Biotechnol. 2: 21), tetracyclines (Suess et al. 2003 Nucleic Acids Res. 31:1853-1858) or dyes (Werstuck and Green, 1998 Science 282:296-298).
So far these switches have been developed with a view to remotely improving the control of heterologous gene expression in host cells and therefore the focus of this research has largely been centered on the development of high-throughput screens or selections to isolate synthetic riboswitches that respond to a variety of exogenously applied ligands (Thompson et al. 2002 BMC Biotechno. 2002 2: 21; Ogawa and Maeda, 2007 Bioorg. Med. Chem. Lett. 17: 3156-3160).
Common gene expression systems continue to suffer drawbacks. One is all-or-nothing expression spread within a population of cells, some are fully induced, others are not. Some promoter based systems are “leaky” meaning that they have high basal (i.e. uninduced) levels of expression. This can present particular difficulties when toxic genes need to be expressed in a controlled way.
The lac and ara systems for example, exhibit cross-talk which makes them difficult to use in stations of simultaneous and differential expression of multiple genes using distinct inducers.
In the tet system, where the inducer is tetracycline or an analog, inhibition of cell growth is often a problem.
Vitamin B12, is controlled by a riboswitch that senses the intracellular concentration of vitamin B12 (see Mandal and Breaker (2004) Nat Rev Cell Biol 5:451-463).
The inventors have for the first time configured an intronic, self-splicing riboswitch for inducible gene expression by introducing an appropriate aptamer, and then used this in an inducible gene expression system, whereby exposure of the cell to the inducer triggers self-splicing of the intron sequence to restore the reading frame of the reporter gene and as such to drive expression of the gene product.
Accordingly, the present invention provides a method of inducing production of a desired RNA molecule in a cell comprising:
The invention also provides a method of inducing expression of a protein or polypeptide in a cell, comprising:
The invention further provides a method of inducing production of a desired RNA molecule in a cell comprising:
Additionally, the invention also provides a method of inducing expression of a protein or polypeptide in a cell, comprising:
Advantageously, the inventors provide an extremely tight (substantially leakage free), inducer-specific expression method and system, in which, for example, a T7 RNA Polymerase gene is interrupted (frame-shifted) by two or more riboswitches; the frameshift is repaired upon self-splicing of the riboswitch(es) in the presence of the inducing ligand; this system may be used together with an induction of transcription, e.g. by IPTG or rhamnose
In the methods of the invention, the host cell may be further transformed with a second expression construct, wherein the second expression construct comprises a polynucleotide sequence whose expression is under the control of a promoter which is controlled or regulated by the RNA, protein or polypeptide product of the first expression construct, and the RNA product of the second expression construct is the desired RNA product. In these embodiments of any aspect of the invention (including methods, systems, transformed cells) as set forth herein, the presence of two expression constructs provides a “two-component” inducible expression system.
In other methods of the invention, the host cell may be further transformed with a second expression construct to provide a two-component system, wherein the second expression construct comprises a polynucleotide sequence whose expression is under the control of a promoter which is controlled or regulated by the RNA, protein or polypeptide product of the first construct, and the protein or polypeptide expression product of the second expression construct is the desired protein or polypeptide.
In certain embodiments of the methods of the invention, the cell may be transformed with the first and second constructs separately. In other embodiments, the cell may be transformed with first and second constructs substantially simultaneously. In yet other embodiments, the cell may be transformed with first and second constructs
In other embodiments of the method of the invention, the cell may contain a second expression construct to provide a two component system, comprising a polynucleotide sequence whose expression is under the control of a promoter which is itself controlled or regulated by the RNA product of the first expression construct, and the transcribed RNA of the second expression construct is the desired RNA,
In further embodiments of the methods of the invention, the cell may contain a second expression construct to provide a two component system, comprising a polynucleotide sequence whose expression is under the control of a promoter which is controlled or regulated by the expressed RNA, protein or polypeptide of the first expression product, and expressed protein or polypeptide of the second expression construct is the desired protein or polypeptide.
In various methods of the invention, the RNA may be selected from one of a microRNA (miRNA), a small interfering RNA (siRNA), an antisense RNA, a tRNA or a ribozyme.
In any of the methods of the invention, the host cell may be a prokaryotic cell, or a eukaryotic cell.
In methods of the invention, the at least two self-splicing introns each comprise an aptamer and wherein the aptamer is the same.
In two-component inducible expression methods of the invention the polynucleotide sequence of the first expression construct may encode Phage T7 DNA dependent RNA polymerase, in which case the second expression construct may comprise the promoter PT7.
The inducer is usually a ligand. In preferred aspects of the methods of the invention, the aptamers bind theophylline which acts as the inducer ligand. Other suitable aptamers may include those which bind to tetracycline, neomycin or malachite green.
In other preferred aspects of the methods of the invention, the self-splicing intron is the T4 td gene self-splicing intron.
The invention also provides a cell for inducer molecule-controlled expression of a gene product, the cell comprising a polynucleotide expression construct, wherein the construct comprises a polynucleotide sequence which is interrupted by at least two introns which are self-splicing introns and whose splicing activity is under the control of an aptamer, and wherein the aptamer has binding affinity for the inducer.
In certain aspects, the expressed gene product may be an RNA molecule; optionally one of a microRNA (miRNA), a small interfering RNA (siRNA), an antisense RNA, a tRNA or a ribozyme.
In other aspects, the expressed gene product may be a protein or polypeptide.
In certain two-part inducible expression embodiments of the invention, the cell may further comprise a second expression construct comprising a polynucleotide sequence whose expression is under the control of a promoter whose activity is regulated by the expression product of the first expression construct.
The expressed product of the second expression construct may be an RNA molecule; optionally one of a microRNA (miRNA), a small interfering RNA (siRNA), an antisense RNA, a tRNA or a ribozyme. The expressed product of the second expression construct may alternatively be a protein or polypeptide.
In cells of the invention, the two or more self-splicing introns preferably contain the same aptamer, each having the same binding affinity for the inducer.
In preferred aspects, the or each self-splicing intron is the T4 td gene self-splicing intron.
The invention also provides a kit for preparing an inducible expression construct for the expression of a gene product in a cell, comprising:
The invention further provides a kit for preparing an host cell for inducible host cell expression of a gene product, comprising:
Also, the invention provides a kit for inducible expression of a gene product, comprising:
In certain embodiments of kits of the invention, the self-splicing intron is preferably the T4 td gene self-splicing intron.
Any of the kits of the invention may further comprise a container containing an inducer; optionally wherein the inducer ligand is selected from one of theophylline, tetracycline, neomycin or malachite green.
The utility of the present invention resides in the broad applicability of the methods of inducible gene expression, the polynucleotide expression vectors, transformed cells and kits; whereby any desired nucleic acid sequence can be tightly expressed under the control of an inducer molecule, in any commonly used host cell, for example prokaryotic cells, fungal cells, plant cells or animal cells.
The inducible expression methods and systems and materials of the invention are applicable for the controlled expression of any protein or polypeptide of interest; preferably tight on/off control substantially without leakage, i.e. expression in the absence of inducer ligand. Proteins of interest may typically include polypeptide macromolecules comprising 20 or more contiguous amino acid residues and may include, but are not limited to enzymes, structural proteins, binding proteins and/or surface-active proteins.
Primary Inducible Expression Constructs
As described-above, the methods, systems, cells and kits of the invention employ as an essential feature a primary inducible expression construct. The primary construct comprises a polynucleotide sequence encoding an RNA molecule, a protein or a polypeptide, and wherein the polynucleotide sequence encoding these is interrupted by at least two introns, which are self-splicing introns and whose splicing activity is under the control of an aptamer, and wherein the aptamer has binding affinity for an inducer. An advantage of such constructs is that their transcription of the desired nucleic acid sequence (which contains the self-splicing introns) is only switched on in the presence of an inducer which binds to the aptamers of the self-splicing introns. This results in self-splicing activity and the generation of the relevant RNA transcript.
A further advantage arising out of the at least two self-splicing introns with aptamers for specific binding of an inducer is that there is substantially no background level translation of the desired RNA transcript, and in circumstances where the RNA transcript is translated by the cellular machinery, then substantially no background level expression of a desired protein or polypeptide. In other words, the primary inducible expression construct element of any aspect of the invention provides a tight on switch, not susceptible to background transcription or background expression of the desired protein or polypeptide beforehand. Similarly, in the absence of inducer following a period of induction, there is a fight off switch resulting in a rapid and substantial cassation of transcription or expression activity.
The expression product of the primary inducible expression construct may be an intermediate in an overall system for expressing a desired gene product; in the sense that the intermediate expression product acts on a promoter or regulatory element of a second expression construct which itself transcribes/expresses the desired RNA or protein/polypeptide gene product in these embodiments, the invention provides methods, systems and cells which are a two-part inducible expression system, further improving the substantial absence of background expression levels and continuing to provide a tight on/off switch controlled by the presence of suitable concentration of inducer.
Host Cells
Advantageously, the present invention is of broad applicability and host cells of the present invention may be derived from any genetically tractable organism which can be cultured. Therefore, in particular, commonly used host cell may be selected for use in accordance with the present invention including prokaryotic or eukaryotic cells which are genetically accessible and which can be cultured. The approaches defined herein for the selection of cells which express a protein of interest may be applied to those cells which are able to serve as a host for production of the protein of interest (POI)). It may therefore be applied to commonly used host cells, for example prokaryotic cells, fungal cells, plant cells and animal cells commonly used for recombinant heterologous protein expression.
Appropriate host cells may be prokaryotic or eukaryotic. Preferably, host cells will be selected from a prokaryotic cell, a fungal cell, a plant cell, a protist cell or an animal cell. Preferred host cells for use in accordance with the present invention are commonly derived from species which typically exhibit high growth rates, are easily cultured and/or transformed, display short generation times, species which have established genetic resources associated with them or species which have been selected, modified or synthesized for optimal expression of heterologous proteins under specific conditions. In preferred embodiments of the invention where the protein of interest is eventually to be used in specific industrial, agricultural, chemical or therapeutic contexts, an appropriate host cell may be selected based on the desired specific conditions or cellular context in which the protein of interest is to be deployed. Preferably the host cell will be a prokaryotic cell. In preferred embodiments the host cell is a bacterial cell. Preferably the host cell is an Escherichia coli (E. coli) cell.
Expression Vectors
The primary and any second inducible expression construct can vary according to the recipient host cell and suitably may incorporate regulatory elements which allow expression in the host cell of interest and preferably facilitate high-levels of expression upon induction. Such regulatory sequences may be capable of influencing transcription or translation of a gene or gene product, for example in terms of initiation, accuracy, rate, stability, downstream processing and mobility.
Such elements may include, for example, strong and/or constitutive promoters, 5′ and 3′ UTR's, transcriptional and/or translational enhancers, transcription factor or protein binding sequences, start sites and termination sequences, ribosome binding sites, recombination sites, polyadenylation sequences, sense or antisense sequences, sequences ensuring correct initiation of transcription and optionally poly-A signals ensuring termination of transcription and transcript stabilisation in the host cell. The regulatory sequences may be plant-, animal-, bacteria-, fungal- or virus-derived, and preferably may be derived from the same organism as the host cell. Clearly, appropriate regulatory elements will vary according to the host cell of interest. For example, regulatory elements which facilitate high-level expression in prokaryotic host cells such as in E. coli may include the pLac, T7, P(Bla), P(Cat), P(Kat), trp or tac promoters. Regulatory elements which facilitate high-level expression in eukaryotic host cells might include the AOX1 or GAL1 promoter in yeast or the CMV- or SV40-promoters, CMV-enhancer, SV40-enhancer, Herpes simplex virus VIP16 transcriptional activator or inclusion of a globin intron in animal cells. In plants, constitutive high-level expression may be obtained using, for example, the Zea mays ubiquitin 1 promoter or 35S and 19S promoters of cauliflower mosaic virus (CaMV).
Suitable regulatory elements may be constitutive, whereby they direct expression under most environmental conditions or developmental stages or developmental stage specific.
In the secondary expression constructs or vectors of the invention, there is preferably an inducible promoter. The inducer is the transcription product (an RNA molecule) or an expression product (protein or polypeptide) of the primary inducible expression construct. What this provides is a two-component inducible expression system.
Transformation of Host Cell with the Expression Constructs
Expression constructs (primary or secondary) may be located in plasmids (expression vectors) which are used to transform the host cell. Methods of transformation may include but are not limited to; heat shock, electroporation, particle bombardment, chemical induction, microinjection and viral transformation.
A host cell may first be transformed with a primary inducible expression construct, and then followed by transforming the cell with the second expression construct. Alternatively the host cell is transformed substantially simultaneously with the primary construct and the secondary construct.
Throughout, the term “polynucleotide” as used herein refers to a deoxyribonucleotide or ribonucleotide polymer in single- or double-stranded form, or sense or anti-sense, and encompasses analogues of naturally occurring nucleotides that hybridize to nucleic acids in a manner similar to naturally occurring nucleotides. Such polynucleotides may be derived from any organism, including the host organism, or may be synthesised de novo. The provision of a polynucleotide may comprise synthesis of a polynucleotide. This may be for example by modification of a pre-existing sequence, e.g. by site-directed mutagenesis or possibly by de novo synthesis.
In all embodiments of the invention, polynucleotide sequences encoding the RNA, protein or polypeptide of interest may be prepared by any suitable method known to those of ordinary skill in the art, including hut not limited to, for example, direct chemical synthesis or cloning for introduction into a desired host cell. Alternatively, the starting polynucleotide sequence may be provided and subsequently modified ex vivo or alternatively in vivo for example by site directed mutagenesis or gene editing techniques.
Self-Splicing Introns
Advantageously, methods, systems and kits of the present invention which employ just a primary expression construct or vector are based on having a gene of interest to be transcribed/expressed in a transformed host cell, and which is interrupted by two or more riboswitches with adjustable ribonuclease (RNase) activity and/or adjustable RNA ligase activity; i.e. “self-splicing introns”. When inducer is applied to the host cell it binds to each of the aptamers thereby resulting in self-splicing intron activity.
When self-splicing of the mRNA transcript occurs, this restores the open reading frame that results in functional expression of a desired protein or polypeptide.
Therefore, in the presence of an inducer, splicing of each intron will be induced, restoring the reading frame of the polynucleotide of interest, resulting in faithful translation and therefore expression of the desired protein/polypeptide.
Aptamers
Aptamers as used in the present invention are polynucleotide sequences which have a high binding affinity for the inducer used. The aptamers may be DMA, cDNA, RNA, preferably RNA. Suitable aptamers of the present invention are preferably 20-30 nt in length; optionally they are 20 nt, 21 nt, 22 nt, 23 nt, 24 nt, 25 nt, 26 nt, 27 nt, 28 nt, 29 nt or 30 nt in length.
Advantageously, the present invention makes use of tandem self-splicing riboswitches; stretches of RNA that can adopt different conformational states, depending on the presence or absence of an inducing molecule which binds to an aptamer portion of each riboswitch. In their natural function, riboswitches are not usually required to completely switch off expression of their genes and therefore the control of gene expression exerted by natural riboswitches is known to be incomplete. However, background levels of cell survival, growth and/or marking of the cells (where visual detection of a reporter is used) due to incomplete riboswitch control of expression (for example in the absence of the binding product), negatively influences the efficiency of inducible expression systems.
For the purpose of developing an accurate screening or selection system, a lightly controlled on/off switch is desirable. Advantageously, the present invention employs synthetic riboswitches with improved stringency, whereby two, optionally more than two, i.e. multiple copies of the riboswitches in sequential arrangement are used.
The self-splicing riboswitches whose splicing is under the control of an aptamer, have the effect in use of reducing background levels of expression of genes into which they have been introduced. Reductions in background level expression compared to the UTR located riboswitch constructs or existing known two-component expression systems is preferably at least about 50% (i.e. only about 50% of the background level expression of the existing constructs or systems), more preferably at least about 75%, more preferably at least about 80% reduction.
When two self-splicing introns are present as the riboswitches under aptamer control, then when compared to equivalent constructs and systems where there is just one riboswitch under the aptamer control, then the background level of growth of cells is reduced, by at least 75%, at least 80%, at least 85%, at least 90%, at least 95%. Reductions of at least about 99.5%, at least about 99.9% or even 100% (i.e. no background level expression) are optionally preferred.
The plasmid encoded thyA gene may be interrupted by a theophylline responsive self-splicing intron; single or in tandem. The pSC018-Theo contains one intron in the coding sequence and does slow down the growth significantly when not induced, however during prolonged incubation (overnight) the non-induced bacteria grow to a similar density as the induced bacteria. While induction does give a growth advantage, the selection is not black and white. A single-intron insertion on another position (
The invention will now be described in detail with reference to the examples and to the drawings in which:
An in vivo biosensor is usually composed of a control element and a reporter gene. The reporter gene can confer antibiotic resistance, fluorescence, auxotrophy complementation or luminescence. The control element can act on several stages in the protein production process. Protein based control elements like LacI typically intervene with the transcription of the gene, while inteins and post-translational modification deal with activation of the protein itself. In between, there is the control on translational level predominantly performed by riboswitches. The riboswitches are mostly located in the 5′-UTR sequestering and releasing the Shine-Dalgarno sequence to block or allow translation by the ribosome. For example,
It may be difficult to alter the ligand of these riboswitches, since the anti-Ribosome Binding Site (anti-RBS) or anti-terminator may be part of the aptamer domain. Altering the aptamer domain will change the anti-RBS or anti-terminator rendering the riboswitch inactive. Randomising the 5′-UTR and testing for riboswitch activity is one solution to that problem. Another possibility is changing a ribozyme, either a synthetic or a natural one, into a riboswitch by attaching an aptamer domain. This creates an allosteric ribozyme also referred to as aptazyme. An aptazyme that was based on the hammerhead ribozyme was designed by Ogawa and Maeda (2007) Bioorg Med Chem Lett 17:3156-3160.) (This aptazyme is based on SD sequestering and the block is released by the endonuclease activity of the ribozyme upon induction. A different approach using the same mechanism can be applied in eukaryotes cutting of the poly-A tail upon induction. A type of synthetic riboswitch that has not been studied extensively is the group I aptazyme. This aptazyme is a modified version of a group I self-splicing intron. The intron that was modified is derived from the phage T4 td gene encoding thymidylate synthase (see
This system has many properties that make it suitable as an in vivo biosensor. Contrary to riboswitches that block the Ribosome Binding Site (RBS), there is only one way leakage can occur; when blocking the RBS the block may be released by complete unfolding of part of the mRNA. When the intron is unfolded it does not splice out of the mRNA, still disallowing functional translation. The leakage that will occur in both instances is when the aptamer is not completely destabilised when no ligand is present and the switch is flipped in the absence of a trigger. The gene the intron is naturally present in is an essential gene that can be complemented easily by adding thymidine to the medium or supplying the gene on a plasmid. By default, no large amount of thymidine is present in most widely used media like LB, so all experiments can be performed on rich media. The selection allows for a great number of variants to be tested without expensive equipment or labour intensive experiments. A property the td intron based riboswitch shares with other riboswitches is the transferability to other organisms, as riboswitches are unaffected by post-translational modification. Group I introns have the extra advantage that no species-specific elements like SD sequence or poly-A tail are involved. This experiment focuses on the exact conditions the theophylline responsive T4 td intron needs to function as selection tool in E. coli.
Auxotrophy Complementation with thyA Under Control of a Theophylline Dependent intron
thyA is a gene encoding thymidylate synthase, a crucial part of the pyrimidine synthesis pathway. It catalyses the reaction from dUMP to dTMP using THF as a cofactor (see
The relationship between the growth rate of single intron constructs (i.e. those with a single self-splicing intron) and promoter strength was determined.
The theophylline dependent phage T4 td intron was designed according to Thompson et al (2002) BMC Biotechnol 2: 21. The intron flanking regions are identical on protein level between the thyA gene of E. coli and the td gene of phage T4 allowing for introduction of the intron into thyA with silent mutations only.
Reporter constructs carry a p15A origin of replication derived from pACYC184, a kanamycin resistance gene from pET24d and the 5′-UTR and CDS of E. coli thyA. A terminator and promoters of different strength were placed upstream of the 5′-UTR. All prompters are listed in Table 1. E. coli DH10B-ΔthyA carrying the reporter constructs were grown with different amounts of theophylline and monitored for 20 h.
The log phase growth rate was determined in biological triplicate for each construct and theophylline concentration (
The constructs with promoters PtacI (Δ) and Ptet (O) show near maximum growth while not being induced and maximum growth with slight induction. The PlacUVS (⋄) construct shows the highest theophylline dependency having no growth without induction and a dynamic range of 0 mM-0.4 mM theophylline resulting in growth rate 0 h−1-0.69 h−1. The promoters Para, Pbla, Pcat and Plac do not support log phase growth nor does the negative control (frame shifted thyA). E. coli DH10B containing the frame-shifted thyA serves as positive control (closed square) (
In all cases a higher thyA expression results in more growth. More growth does not necessarily mean that the ThyA expression is high enough to sustain the growth. No growth above OD600 of 0.040 was observed for bacteria carrying thyA under control of the Para, Pbla, Pcat and Plac promoters. These promoters do not support log phase growth, but can extend the period the bacteria can grow on the carry-over thymidine depending on the promoter strength and induction by theophylline.
Theophylline dependent log phase growth was observed for PlacUVS, PtacI and Ptet. These promoters more closely resemble the consensus sequence of the −35 and −10 regions. To observe better growth when the auxotrophy complementation is under control of a stronger promoter is to be expected. The promoters PtacI and Ptet are strong enough to support log phase growth without induction by theophylline, while the PlacUVS promoter does not. The positive control, E. coli DH10B with the frame-shifted thyA gene, appears to be slightly theophylline dependent. This effect is marginal and is most likely caused by the position the samples have on the microtiter plate rather than the theophylline concentration (
Auxotrophy complementation indirectly depends on the concentration of mature mRNA. The concentration of mature mRNA depends on the concentration of immature mRNA and the maturation rate. The concentration of immature mRNA is mostly dependent on promoter strength, while the maturation rate is dependent on theophylline induction. The maturation rate does not equal zero when the no inducer is present. This leakage is shown by the constructs having a strong promoter in front of the coding sequence. Where there no leakage, promoter strength should have no effect when no inducer is present. The weak promoter constructs do not generate enough mature mRNA even when the maturation rate is high. The amount of ThyA is not enough to reach the minimal concentration of dTTP required in the cell. A concentration below the minimal requirement will result in thymineless death. It appears there is a fine line between never enough ThyA and always enough ThyA. The balance is matched rather well with the PlacUV promoter. No growth is observed when no inducer is present and maximum growth is observed at full induction (
Although the growth of E. coli DH10B-ΔthyA carrying the PlacUVS construct was not observed in microtiter plates, it sometimes was observed in 5 mL cultures in a 50 mL Greiner tube. Evaporation is a serious issue in the microtiter plate only causing problems after several hours of growth. By that time all exponential growth was finished already and carry-over thymidine was consumed staggering the growth. The bacteria in the Greiner tube did not suffer from evaporation, so a very small subpopulation having slightly increased expression may become dominant overnight indicating that the background expression of ThyA is only just below the minimal requirement, sometimes exceeding it. While this background growth may not be interfering with competition experiments between induced and uninduced bacteria, it may lead to false negatives.
The strong promoters PtacI and Ptet showed leakage exceeding the minimal requirement of ThyA (
Previously, Pichler et al. (Pichler and Schroeder, 2002, J Biol Chem 277:17987-17993) showed that the flanking sequence does not need to match the native flanking sequence perfectly for a functional phage T4 td intron. Next to some tolerance in the intron flanking regions, the coding sequence can be composed of different codons. An algorithm was written to analyse the thyA coding sequence for possible locations for the intron to be inserted. The position had to match several requirements: 1) Mutations in the coding sequence were to be translationally silent for both the intron flanking regions and the restriction sites to clone the second intron into the thyA gene; 2) The flanking regions of the second intron had to match the flanking regions of the native intron as closely as possible; 3) No mutations in the flanking regions were allowed other than described by Pichler et al. (Pichler and Schroeder, 2002, J Biol Chem 277:17987-17993); and 4) Possibility of silent introduction a restriction site next to either flank was preferential.
The top candidate position was identified as HLRSI (amino acids 51-55) with the intron in frame 2 and only one mutation in the intron flanking region changing a wobble base pair into a U-A base pair. Two unique restriction sites could be mutated close to the insertion site: Psp1406I upstream and PstI downstream. A construct with a tandem intron at (H51-I55) and (F171-P175) and a construct with the (H51-I55) intron only were made. In both cases, the thyA gene was under control of the PtacI promoter. The constructs were tested in E. coli DH10B-ΔthyA according to the same protocol as the single intron constructs (
The growth rate of both single and double intron constructs under control of the PtacI promoter was measured (
A reduction of background was discovered with the second intron (
The phage T4 td intron is therefore a useful tool for selection of E. coli that have a small molecule inside their plasma membrane. The ability of this system to completely select against bacteria that have no such small molecule present makes it relatively straightforward to select for the bacteria that do. Leakage and fully-induced expression can be carefully adjusted so bacteria without small molecule do not grow at all, whilst the bacteria with small molecule do. It was shown that the PlacUVS promoter can balance the leakage and the induced expression so that the dynamic range is between 0 mM and 0.4 mM theophylline resulting in a growth rate between 0 h−1 and 0.69 h−1 on microtiter plate. However, this particular promoter is not expected to support Log phase growth and is net so preferred. A direct route to manage balance between leakage and full expression is the introduction of a second intron at an upstream position. Tandem introns are significantly more effective in reducing background splicing, while maintaining the dynamic range in both inducer concentration and growth rate.
Materials and Methods
Chemicals and Plasmids
Thymidine and theophylline were purchased from Sigma-Aldrich (St. Louis, Mo.). A plasmid containing the E. coli thyA gene interrupted by a modified phage T4 td intron between G173 and L174 was commissioned at GeneArt (pMA-ThyA-SI001) as well as an intron version containing a theophylline responsive aptamer (pMA-ThyA-Theo). Plasmid pET24d was purchased from Novagen. Plasmid pRham C-His was purchased from Lucigen.
Enzymes were purchased from Thermo Scientific and used according to the manufacturer's instructions, unless stated otherwise.
Bacterial Strains and Media
E. coli DH10B T1R was purchased from invitrogen (C6400-03) and used for plasmid propagation and standard molecular techniques, as well as a parent strain for the thyA deficient E. coli DH10B-ΔthyA strain. Transformation was performed with a ECM 63 electroporator (BTX) at 2500 V, 200 Ω and 25 μF, 2 mm cuvettes, 20-40 μL of electro-competent cells and recovery in LB.
Bacteria were generally grown at 37° C. on LB medium (Miller) containing the appropriate antibiotics: kanamycin (50 mg/L), ampicillin (100 mg/L), chloramphenicol (35 mg/L) and tetracycline (15 mg/L). In addition, the auxotrophic E. coli DH10B-ΔthyA was complemented with thymidine (100 mg/L) when necessary.
Construction of Reporter Plasmids
The reporter plasmids pSC018a-g—Theo were constructed using pACYC184 as a base. The steps include exchange of the chloramphenicol acetyltransferase (cat) for the aminoglycoside 3′-phosphotransferase (kan) from pET24d (Novagen), exchanging the TetA(C) for the thyA gene encoded on the pMA-ThyA-SI001 plasmid and exchanging the 6b hairpin for the theophylline responsive aptamer from pMA-ThyA-Theo).
Promoter variants were made by polymerase chain reaction (PCR) and ligating the PCR product into pSC018f-Theo (
DNA purification was performed with the DNA Clean & Concentrator-5 kit of Zymo Research (D4004) or the Zymoclean™ Gel DNA Recovery Kit (D4002). Plasmid was isolated with the Plasmid Miniprep kit of Thermo Scientific (#K0503). Ligation was performed at 22° C. for 1 h, followed by 10 min heat inactivation. All plasmids were verified by PCR and/or restriction analysis and sequencing by GATC Biotech (Konstanz, Germany).
Construction of the Thymidine Synthase Deficient Strain
The thyA deficient strain DH10B-ΔthyA was made according to a standard protocol (Datsenko and Wanner (2000) PNAS 97: 6640-6645) with the exception of the PCR template and the competent cells protocol and the PCR template for the insertion cassette.
Electro-competent cells were made by growing DH10B T1R (Invitrogen) containing pKD46 at 30° C. on 16 g/L peptone, 10 g/L yeast extract and appropriate antibiotic to an OD600 of 0.4 and cooled down to 4° C., washed with ultrapure water once and 10% glycerol twice. Finally the bacteria were concentrated 250× in 10% glycerol.
DH10B T1R containing pKD46 was transformed with a PCR product generated from pMA-RQ-Lox71-kan-Lox66, kindly provided by Teunke van Rossum, containing a kanamycin resistance gene flanked by Lox71 and Lox66. The Lox sites can be recombined by cre recombinase removing kanamycin resistance, but do not form a functional Lox site. Transformed bacteria were recovered in LB medium containing thymidine (100 mg/L) for 2.5 h at 37° C. and plated on LB agar plates containing kanamycin (50 mg/L) and thymidine (20 mg/L). Colonies were verified for thyA deficiency by plating on LB agar plates containing kanamycin (50 mg/L). Plasmid curation was assessed by growing on LB agar plates containing ampicillin (100 mg/L) and thymidine (20 g/L).
Electro-competent cells were made from DH10B T1R-ΔthyA-kan growing on medium containing kanamycin (50 mg/L) and thymidine (100 mg/L) at 37° C. and transformed with pJW168 containing the cre recombinase. Auxotrophy, recombination of the Lox sites and plasmid curation were assessed by plating on LB agar medium, LB agar containing kanamycin (50 mg/L) and thymidine (20 mg/L) and plating on LB agar medium containing ampicillin (50 mg/L) and thymidine (20 mg/L). Electro-competent cells were made of the knock-out strain and transformed with the auxotrophy reporter constructs.
E. coli DH10B-ΔthyA Growth Assays
E. coli DH10B-ΔthyA containing a reporter construct of the pSC series were grown overnight at 37° C. on LB medium containing kanamycin (50 mg/L) and thymidine (100 mg/L). A 10−4 dilution was made and grown in with a variable amount of theophylline in a 96 well microtiter plate (Greiner) in a final volume of 200 μL. Culture plates were incubated under continuous shaking for 20 h at 37° C. and the OD600 was measured every 10 minutes in a Synergy MX plate reader. As carry-over thymidine allows the knock-out strain to grow without ThyA, a lower limit OD600 was set to 0.040 AU to negate false positive growth. Growth rate (μ) was calculated from at least 1 h of log phase growth exceeding an OD600 of 0.040 according to
In(C)=In(C0)·μ·t
Group I self-splicing introns are RNA molecules with catalytic activity: i.e. RNA ribozymes. These introns catalyze their own excision from precursors such as mRNA. The well characterized T4 self-splicing intron has been demonstrated to adopt a specific 3D-structure that is required for catalytic activity (
The T4 self-splicing intron has been engineered into a functional catalytic riboswitch by inserting a theophylline-binding aptamer (
To test for riboswitch functionality, the intron/aptamer fusion was integrated in a reporter gene. Next to the aforementioned antibiotic resistance marker, also auxotrophic markers can be used (essential genes for amino acids or nucleotides are deleted in microbial hosts; growth in the absence of these amino acids or nucleotides is only possible when the corresponding gene is complemented in a plasmid). For the riboswitch test, an E. coli thyA knockout strain; the thyA gene encodes an essential enzyme in biosynthesis of thymidine (one of the four bases of DNA nucleotides). The thymidine auxotrophy is complemented by a plasmid-borne thyA gene. When using a plasmid with ThyA that was interrupted by the hybrid riboswitch, it was demonstrated that the thyA knockout strain of E. coli could survive on minimal medium containing theophylline (without thymidine), but not on minimal medium lacking both theophylline and thymidine (Thompson at al. 2002 BMC Biotechnol. 2: 21). Hence, the presence of the riboswitch ligand theophylline, allows for growth.
In the aforementioned experiments (Thompson et at 2002 BMC Biotechnol, 2: 21), there was a background level cell growth was observed in the absence of the theophylline ligand (Figure ). This background was subtracted from the growth observed in the presence of the ligand. Theophylline-induced growth was approximately 40% of the parental intron, so maximally 40% of the wild type growth. In an experiment based on the latter publication, the induction of the thyA gene by theophylline turned out to be far from stringent. Non-induced splicing of the intron was thought to be the cause of growth on minimal medium lacking both theophylline and thymidine. As a solution to the problem of non-induced splicing, a suitable second insertion site was identified, for the insertion of a second self-splicing intron.
A comparison of a single intron construct with a construct of the present invention featuring a tandem self-splicing intron was made (
The introduction of a “tandem-riboswitch” resulted in a 70% recovery of growth compared to wild type with theophylline induction, while no growth at all was observed without induction by theophylline, i.e. black and white selection.
Each mRNA only possesses a single 5′ UTR and this limits the potential positions for riboswitches to be inserted. It has been shown that the introduction of more than one riboswitch can confer improved stringency on the activity of the riboswitch, by removing background levels of non-induced splicing (and therefore expression). As there is only one 5′ UTR and multiple positions for an intron, instead of inserting the riboswitch in the 5′ UTR, use of the coding sequence allows multiple riboswitches to be inserted into the coding sequence and therefore improved flexibility and stringency of the engineered switch. The presence of a specific ligand (e.g. theophylline) controls self-splicing of the riboswitch, thereby restoring the reading frame of the gene encoding the desired gene product.
Auxotrophy complementation is based on interruption of an important step in the pyrimidine synthesis pathway (
The plasmid encoded thyA gene is interrupted by a theophylline responsive self-splicing intron; single or in tandem. The vector maps are depleted in
Being an enzyme, a single molecule of ThyA can convert a vast amount of dUMP to dTMP, thereby enhancing the signal. For other reporters, like GFPuv, this is not the case as the bacteria are only as fluorescent as there are GFPuv molecules. Signal below the detection limit is a problem in case of GFPuv as is illustrated in Table 2. The fluorescence of the bacteria themselves (pSC012) is in the same order of magnitude as the induction independent self-splicing intron (pSC034f-SI001). The induction by theophylline is not significantly observed in pSC034f-Theo. Possibly there is a difference in expression between the induction independent self-splicing intron and the non-induced and induced theophylline dependent intron, but it cannot be concluded from these data. When no intron is present in the GFPuv (pSC034f) the fluorescence is multiple orders of magnitude higher than the background fluorescence.
To enhance the signal from GFPuv, a cascade was made using DNA dependent RNA polymerase from phage T7. The GFPuv expression is controlled by T7 polymerase and the T7 polymerase is in turn controlled by the theophylline responsive intron (
A few copies of the T7 polymerase will result in a myriad of GFPuv molecules, so a small change in T7 polymerase concentration will result in a large change in GFPuv concentration, which can be measured. Since the T7 polymerase is a very processive enzyme, it needs a tight control of expression.
The reporter plasmid pSC028-GFPuv-term was constructed using pACYC184 as a base (vector map shown in
The performance of the cascade was measured using GFPuv expression (
It is unlikely that an enzyme of interest will provide a concentration as high as 1 mM for every small molecule that is screened. As the enzyme of interest functions inside the cell, the cell membranes acting as a harder will help the production of GFPuv rather than diminishing it. Usually aptamers have a dissociation constant in the low μM range, so for maximum signal the intracellular theophylline concentration does not have to be 1 mM, but much less.
Construction of Reporter Plasmids
The reporter plasmid pSC028-GFPuv-term (
The plasmid pRham-CHis (Lucigen) was used as base for constructing the T7 polymerase variants. The CDS is flanked by an NdeI site and 6×His tag on the 5′-end and a BgIII site on the 3′-end. The intron positions are between G201 and L202 flanked by PscI and HindIII, between G449 and L450 flanked by Bsu15I and XagI and between G671 and L672 flanked by Eco88I and PstI. All have CAAGGGT as 5′ intron flank instead of wild type CTTGGGT. The 3′ intron flanks are CTAC, CTAC and CTAA respectively.
GFPuv Fluorescence
E. coli DH10B-T7His-Theo4 was grown overnight at 37° C. in LB medium containing kanamycin (50 mg/L). A 96 well 2 ml culture plate (Greiner) was filled with a concentrate of theophylline and L-rhamnose. LB medium containing kanamycin and overnight grown bacteria were added so that the final concentration of kanamycin was 50 mg/L, the bacteria had a final dilution of 10−3 and the theophylline and L-rhamnose were diluted to 1× in 500 μL total volume. Culture plates were incubated at 37° C. overnight under continuous shaking. The bacteria were centrifuged for 10 minutes at 4700 rpm in a Sorval Legend centrifuge. The supernatant was cleared and the cell pellet was resuspended in 500 μL 50 mM Tris-HCl pH 7.5. After resuspension, the plates were incubated at 37° C. for 1 hour to allow maturation of the GFPuv. 100 μL of suspension was pipetted into a 98 well black plate with clear bottom (Perkin Elmer) and measured with a Synergy MX plate reader. The cell density was measured by scattering at 600 nm and the fluorescence was measured at an excitation wavelength of 385 nm with a width of 20 nm and an emission wavelength of 508 nm with 20 nm width with a gain of 50. The background fluorescence and background scattering were subtracted and the fluorescence was divided by the scattering at 600 nm. The background fluorescence of bacteria without either GFPuv or T7His polymerase was negligible, but the fluorescence caused by other components than GFPuv in the bacteria was still subtracted.
Results
The theophylline dependency of the cascade in response to differing concentrations of rhamnose was measured. E. coli DH10B-T7His-Theo4 diluted from an overnight culture were grown overnight in a 2 mL culture plate containing a variable amount of L-rhamnose and theophylline. The medium was cleared and the bacteria were resuspended in 50 mM Tris-HCl pH 7.5. The fluorescence was measured at an excitation wavelength of 385 nm and an emission wavelength of 508 nm. The cell density was measured by scattering at 600 nm.
GFPuv fluorescence showed a strong dependency on both L-rhamnose and theophylline (
Since the output dynamic range can be adjusted relatively easily, the intron controlled T7His polymerase can be employed as a generic tool. The intron lowers the maximum translation quite severely, so not all reporter genes will show enough signal when put under control of a ligand dependent intron directly. Enzymes like ThyA or LacZ can handle the lower translations efficiency, but all genes that need an at least decent expression to function, like GFPuv, can now be put under control of one enzyme. An additional advantage is the exchangeability of the reporter plasmids. Expression from these reporter plasmids can be easily adjusted by mutating the T7 promoter.
| Number | Date | Country | Kind |
|---|---|---|---|
| 1506507.1 | Apr 2015 | GB | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2016/058383 | 4/15/2016 | WO | 00 |
