Claims
- 1. A single stranded antisense riboprobe comprising at most about 498 nucleotides containing an RNA sequence of 222 nucleotides fully complementary to human c-fes exon-2.
- 2. A single stranded antisense riboprobe containing an RNA sequence of 222 nucleotides fully complementary to human c-fes exon-2 wherein said riboprobe specifically hybridizes to human nucleic acid comprising c-fes exon-2 sequences but which does not specifically hybridize to human nucleic acid lacking c-fes exon-2 sequences.
- 3. A kit for the detection of human c-fes RNA comprising a container containing the probe of claim 1 or 2.
- 4. A method for detecting the presence of human c-fes RNA in a biological sample comprising contacting the RNA present in said biological sample with the antisense riboprobe of claim 1 or 2 wherein the presence of specific hybridization between said antisense riboprobe and human c-fes exon-2 sequence indicates the presence of human c-fes RNA in said biological sample.
- 5. The antisense riboprobe of claim 1 or 2 wherein said antisense riboprobe is produced by transcription of a plasmid containing a DNA sequence located upstream and in opposite orientation to a bacteriophage T7 promoter.
- 6. The antisense riboprobe of claim 1 or 2 wherein said antisense riboprobe further comprises a detectable label.
- 7. The antisense riboprobe of claim 6 wherein said detectable label is selected from the group consisting of a radionuclide, biotin, a fluorescent label and an immunohistochemical agent.
Parent Case Info
This is a divisional of U.S. application Ser. No. 08/252,136, filed May 31, 1994, issued Mar. 9, 1999 as U.S. Pat. No. 5,879,882, which is a continuation of U.S. application Ser. No. 07/954,427, filed Sep. 30, 1992, now abandoned, which is a continuation of U.S. application Ser. No. 07/355,207, filed May 22, 1989, now abandoned.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4766072 |
Jendrisak et al. |
Aug 1988 |
|
5968825 |
Ferrari et al. |
Oct 1999 |
|
Non-Patent Literature Citations (9)
Entry |
Feldman et al., Oncogene Research 1(4) : 441-458 (Sep.-Oct. 1987).* |
Matthews et al., Analytical Strategies for the Use of DNA Probes. Analytical Biochemistry 169 : 1-25 (1988).* |
The Stragene Catalog p. 39 (1988).* |
Avers, Molecular Cell Biology, Addison-Wesley Publishing Company (1986). |
Promega Catalog and Applications Guide, p. 8 and 18 (1988). |
Roebroek et al., The EMBO Journal 4(11)2897-2903 (1985). |
Melton et al., Nucleic Acids Research, 12(18):7035-7056 (1994). |
DeBold et al., Endocrinology 66(6):2648-2657 (1988). |
Watson et al., Molecular Biology of the Gene, Benjmain/Cummings Publishing Co., Inc. (1987). |
Continuations (2)
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Number |
Date |
Country |
Parent |
07/954427 |
Sep 1992 |
US |
Child |
08/252136 |
|
US |
Parent |
07/355207 |
May 1989 |
US |
Child |
07/954427 |
|
US |