Patent Application
20230203004
The invention concerns compounds having antiviral activity, in particular having an inhibitory activity on the replication of the respiratory syncytial virus (RSV). The invention further concerns pharmaceutical compositions comprising these compounds and the compounds for use in the treatment of respiratory syncytial virus infection.
Human RSV or Respiratory Syncytial Virus is a large RNA virus, member of the family of Pneumoviridae, genus Orthopneumovirus together with bovine RSV virus. Human RSV is responsible for a spectrum of respiratory tract diseases in people of all ages throughout the world. It is the major cause of lower respiratory tract illness during infancy and childhood. Over half of all infants encounter RSV in their first year of life, and almost all within their first two years. The infection in young children can cause lung damage that persists for years and may contribute to chronic lung disease in later life (chronic wheezing, asthma). Older children and adults often suffer from a (bad) common cold upon RSV infection. In old age, susceptibility again increases, and RSV has been implicated in a number of outbreaks of pneumonia in the aged resulting in significant mortality.
RSV has been classified in two antigenic subtypes: A and B, with subtype A typically associated with more severe symptoms. Infection with a virus from a given subgroup does not protect against a subsequent infection with an RSV isolate from the same subgroup in the following winter season. Re-infection with RSV is thus common, despite the existence of only two subtypes, A and B.
Today only two drugs have been approved for use against RSV infection. A first one is ribavirin, a nucleoside analogue that provides an aerosol treatment for serious RSV infection in hospitalized children. The aerosol route of administration, the toxicity (risk of teratogenicity), the cost and the highly variable efficacy limit its use. Synagis® (palivizumab a monoclonal antibody, is used for passive immunoprophylaxis. Although the benefit of Synagis® has been demonstrated, the treatment is expensive, requires parenteral administration and is restricted to children at risk for developing severe pathology.
Clearly there is a need for an efficacious non-toxic and easy to administer drug against RSV replication.
Compounds that exhibit anti-RSV activity are disclosed in WO-2015/026792.
The present invention relates to compounds of formula (I)
including any stereochemically isomeric form thereof, wherein
The compounds of the present invention differ structurally over the exemplified compounds in WO-2015/026792 due to the mandatory presence of the R2 substituent as a non-hydrogen substituent. As demonstrated in Example 5.3 the compounds of the present invention have unexpectedly improved antiviral properties against the respiratory syncytial virus (RSV).
As used in the foregoing definitions :
The term “compounds of the invention” as used herein, is meant to include the compounds of formula (I), and the salts and solvates thereof.
As used herein, any chemical formula with bonds shown only as solid lines and not as solid wedged or hashed wedged bonds, or otherwise indicated as having a particular configuration (e.g. R, S) around one or more atoms, contemplates each possible stereoisomer, or mixture of two or more stereoisomers.
Hereinbefore and hereinafter, the terms “compound of formula (1)” and “intermediates of synthesis of formula (1)” are meant to include the stereoisomers thereof and the tautomeric forms thereof.
The terms “stereoisomers”, “stereoisomeric forms” or “stereochemically isomeric forms” hereinbefore or hereinafter are used interchangeably.
The invention includes all stereoisomers of the compounds of the invention either as a pure stereoisomer or as a mixture of two or more stereoisomers. Enantiomers are stereoisomers that are non-superimposable mirror images of each other. A 1:1 mixture of a pair of enantiomers is a racemate or racemic mixture. Diastereomers (or diastereoisomers) are stereoisomers that are not enantiomers, i.e. they are not related as mirror images. If a compound contains a double bond, the substituents may be in the E or the Z configuration. Substituents on bivalent cyclic (partially) saturated radicals may have either the cis- or trans-configuration; for example, if a compound contains a disubstituted cycloalkyl group, the substituents may be in the cis or trans configuration.
The term “stereoisomers” also includes any rotamers, also called conformational isomers, the compounds of formula (I) may form.
Therefore, the invention includes enantiomers, diastereomers, racemates, E isomers, Z isomers, cis isomers, trans isomers, rotamers, and mixtures thereof, whenever chemically possible.
The meaning of all those terms, i.e. enantiomers, diastereomers, racemates, E isomers, Z isomers, cis isomers, trans isomers and mixtures thereof are known to the skilled person.
The absolute configuration is specified according to the Cahn-Ingold-Prelog system. The configuration at an asymmetric atom is specified by either R or S. Resolved stereoisomers whose absolute configuration is not known can be designated by (+) or (-) depending on the direction in which they rotate plane polarized light. For instance, resolved enantiomers whose absolute configuration is not known can be designated by (+) or (-) depending on the direction in which they rotate plane polarized light.
When a specific stereoisomer is identified, this means that said stereoisomer is substantially free, i.e. associated with less than 50%, preferably less than 20%, more preferably less than 10%, even more preferably less than 5%, in particular less than 2% and most preferably less than 1%, of the other stereoisomers. Thus, when a compound of formula (I) is for instance specified as (R), this means that the compound is substantially free of the (S) isomer; when a compound of formula (I) is for instance specified as E, this means that the compound is substantially free of the Z isomer; when a compound of formula (I) is for instance specified as cis, this means that the compound is substantially free of the trans isomer.
Some of the compounds according to formula (I) may also exist in their tautomeric form. Such forms in so far as they may exist, although not explicitly indicated in the above formula (I) are intended to be included within the scope of the present invention.
It follows that a single compound may exist in both stereoisomeric and tautomeric form.
Atropisomers (or atropoisomers) are stereoisomers which have a particular spatial configuration, resulting from a restricted rotation about a single bond, due to large steric hindrance. All atropisomeric forms of the compounds of Formula (I) are intended to be included within the scope of the present invention.
The pharmaceutically acceptable acid addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic acid addition salt forms that the compounds of formula (I) are able to form. These pharmaceutically acceptable acid addition salts can conveniently be obtained by treating the base form with such appropriate acid. Appropriate acids comprise, for example, inorganic acids such as hydrohalic acids, e.g. hydrochloric or hydrobromic acid, sulfuric, nitric, phosphoric and the like acids; or organic acids such as, for example, acetic, propanoic, hydroxyacetic, lactic, pyruvic, oxalic (i.e. ethanedioic), malonic, succinic (i.e. butanedioic acid), maleic, fumaric, malic, tartaric, citric, methanesulfonic, ethanesulfonic, benzenesulfonic, p-toluenesulfonic, cyclamic, salicylic, p-aminosalicylic, pamoic and the like acids.
Conversely said salt forms can be converted by treatment with an appropriate base into the free base form.
The compounds of formula (I) may exist in both unsolvated and solvated forms. The term ‘solvate’ is used herein to describe a molecular association comprising a compound of the invention and one or more pharmaceutically acceptable solvent molecules, e.g. water or ethanol. The term ‘hydrate’ is used when said solvent is water.
For the avoidance of doubt, compounds of formula (I) may contain the stated atoms in any of their natural or non-natural isotopic forms. In this respect, embodiments of the invention that may be mentioned include those in which (a) the compound of formula (I) is not isotopically enriched or labelled with respect to any atoms of the compound; and (b) the compound of formula (I) is isotopically enriched or labelled with respect to one or more atoms of the compound. Compounds of formula (I) that are isotopically enriched or labelled (with respect to one or more atoms of the compound) with one or more stable isotopes include, for example, compounds of formula (I) that are isotopically enriched or labelled with one or more atoms such as deuterium, 13C, 14C, 14N, 15O or the like.
The compounds of formula (I) of the present invention all have at least one chiral carbon atom as indicated in the figure below by the carbon atom labelled with * :
Due to the presence of said chiral carbon atom, a “compound of formula (1)” can be the racemic form, the (R)-enantiomer, the (S)-enantiomer, or any possible combination of the two individual enantiomers in any ratio. When the absolute (R)- or (S)-configuration of an enantiomer is not known, this enantiomer can also be identified by indicating whether the enantiomer is dextrorotatory (+)- or levorotatory (-)- after measuring the specific optical rotation of said particular enantiomer.
In an aspect the present invention relates to a first group of compounds of formula (I) wherein the compounds of formula (I) have the (+) specific rotation.
In a further aspect the present invention relates to a second ground of compounds of formula (I) wherein the compounds of formula (I) have the (-) specific rotation.
In another aspect, the present invention relates to compounds of formula (I)
including any stereochemically isomeric form thereof, wherein
A first group of compounds are compounds of formula (I) wherein X is CH or CF, in particular X is CH.
A second group of compounds are compounds of formula (I) wherein X is N.
A third group of compounds are compounds of formula (I) wherein R1 is C1-3alkyl, in particular R1 is CH3.
A fourth group of compounds are compounds of formula (I) wherein R1 is cyclopropyl.
A fifth group of compounds are compounds of formula (I) wherein R2 is CH3.
A sixth group of compounds are compounds of formula (I) wherein R2 is cyclopropyl.
A seventh group of compounds are compounds of formula (I) wherein R2 is CHF2.
An eight group of compounds are compounds of formula (I) wherein R10 is hydroxy.
Interesting compounds of formula (I) are those compounds of formula (I) wherein one or more of the following restrictions apply :
A particular group of compounds are compounds of formula (I) wherein X is N; R1 is CH3 or cyclopropyl; and R10 is hydroxy
Another particular group of compounds are compounds of formula (I) wherein X is CH; R1 is CH3 or cyclopropyl; R2 is CH3, CHF2 or cyclopropyl; R3 and R4 are hydrogen; R5 is CF3 or cyclopropyl; R6 is hydrogen or F; R7 is F; R8 is hydrogen or F and R9 is halo; and R10 is hydroxy.
Yet another particular group of compounds are compounds of formula (I) wherein X is N; R1 is CH3 or cyclopropyl; R2 is CH3, CHF2 or cyclopropyl; R3 and R4 are hydrogen; R5 is CF3 or cyclopropyl; R6 is hydrogen or F; R7 is F; R8 is hydrogen or F and R9 is halo; and R10 is hydroxy.
Specific examples of compounds of formula (I) are :
In general compounds of formula (I) can be prepared by an art-known amide bond formation reaction between a carboxylic acid compound of formula (II) and an amine of formula (III) wherein said amide-bond formation may be performed by stirring the intermediate compounds of formula (II) and (III) in an appropriate solvent, such as e.g. acetonitrile, dimethyl acetamide, dichloromethane, tetrahydrofuran, or DMF, optionally in the presence of a base, such as triethylamine, DIPEA (diisopropylamine) DMAP (dimethylaminopyridine), or N-methylmorpholine. The carboxylic acid compound of formula (II) can be used as such or can be converted first into a reactive functional derivative thereof, such as, e.g carbonyl imidazole derivatives, acyl halides or mixed anhydrides. Conveniently a coupling agent such as HATU (1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate), DEPC (diethyl cyanophosphonate), EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide), BOP, PYBOP, HBTU is used. Stirring may enhance the rate of the reaction. The reaction may conveniently be carried out at a temperature ranging between room temperature and the reflux temperature of the reaction mixture.
Other synthetic pathways for preparing compounds of formula (I) have been described in the experimental party as general methods of preparation and specific working examples.
The compounds of formula (I) may further be prepared by converting compounds of formula (I) into each other according to art-known group transformation reactions.
The starting materials and some of the intermediates are known compounds and are commercially available or may be prepared according to conventional reaction procedures generally known in the art.
The compounds of formula (I) as prepared in the hereinabove described processes may be synthesized in the form of racemic mixtures of enantiomers which can be separated from one another following art-known resolution procedures. Those compounds of formula (I) that are obtained in racemic form may be converted into the corresponding diastereomeric salt forms by reaction with a suitable chiral acid. Said diastereomeric salt forms are subsequently separated, for example, by selective or fractional crystallization and the enantiomers are liberated therefrom by alkali. An alternative manner of separating the enantiomeric forms of the compounds of formula (I) involves liquid chromatography using a chiral stationary phase. Said pure stereochemically isomeric forms may also be derived from the corresponding pure stereochemically isomeric forms of the appropriate starting materials, provided that the reaction occurs stereospecifically. Preferably if a specific stereoisomer is desired, said compound will be synthesized by stereospecific methods of preparation. These methods will advantageously employ enantiomerically pure starting materials.
The in vitro antiviral activity against RSV of the present compounds was demonstrated in an antiviral assay as described in the experimental part 5.1 of the description and may also be demonstrated in a virus yield reduction assay. The in vivo antiviral activity against RSV of the present compounds may also be demonstrated in a test model using cotton rats as described in Wyde et al. in Antiviral Research, 38, p. 31 – 42 (1998).
The compounds of formula (I) show antiviral properties. Viral infections preventable or treatable using the compounds and methods of the present invention include those infections brought on by Pneumoviridae and in particular by human and bovine respiratory syncytial virus (RSV).
Therefore the present compounds of formula (I), or a pharmaceutically acceptable acid addition salt thereof, may be used as a medicine, in particular may be used as a medicine for the treatment or prevention of infections brought on by Pneumoviridae and in particular by human and bovine respiratory syncytial virus (RSV).
The present invention also provides the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for the treatment or prevention of infections brought on by Pneumoviridae and in particular by human and bovine respiratory syncytial virus (RSV).
In other aspects, provided are methods of treating a respiratory syncytial virus (RSV) infection in an individual in need thereof comprising administering to the individual a therapeutically effective amount of a compound of formula (I) provided herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula (I) provided herein, or a pharmaceutically acceptable salt thereof. In some embodiments, the individual has one or more symptoms of an RSV infection. In some embodiments, the RSV is RSV Type A. In some embodiments, the RSV is RSV Type B.
Also provided are methods of ameliorating one or more symptoms of an RSV infection in an individual in need thereof comprising administering to the individual a therapeutically effective amount of a compound of formula (I) provided herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula (I) provided herein, or a pharmaceutically acceptable salt thereof. In some embodiments, the symptom is one or more of: coughing, sneezing, runny nose, sore throat, fever, decrease of appetite, irritability, decreased activity, apnea, and wheezing. In some embodiments, the individual has a lower respiratory tract infection. In some embodiments, the individual has bronchiolitis, pneumonia, or croup. In some embodiments, the individual has been diagnosed with an RSV infection. In some embodiments, the RSV is RSV Type A. In some embodiments, the RSV is RSV Type B. In some embodiments, the RSV infection has been confirmed by a laboratory test. In some embodiments, the method further comprises obtaining the results of an RSV detecting laboratory test. In some embodiments, the laboratory test comprises detecting RSV in a nasal sample.
Also provided are methods of preventing an RSV infection in an individual at risk of developing an RSV infection comprising administering to the individual a prophylactically effective amount of a compound of formula (I) provided herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a prophylactically effective amount of a compound of formula (I) provided herein, or a pharmaceutically acceptable salt thereof. In some embodiments, the individual is between 0 and about 2 years of age. In some embodiments, the individual was born prematurely. In other embodiments, the individual is greater than 65 years of age. In some embodiments, the individual is immunocompromised.
As used herein, “treatment” or “treating” is an approach for obtaining beneficial or desired results including clinical results. For example, beneficial or desired results in treating a viral infection include, but are not limited to, one or more of the following: eliminating or lessening the severity of one or more symptoms resulting from the viral infection (such as but not limited to coughing, sneezing, runny nose, sore throat, fever, decrease of appetite, irritability, decreased activity, apnea, and wheezing), increasing the quality of life of those suffering from the viral infection, decreasing the dose of other medications required to treat the viral infection, delaying the progression of the viral infection, and/or prolonging survival of an individual.
As used herein, “preventing” a viral infection is an approach for eliminating or reducing the risk of developing a viral infection or delaying the onset of a viral infection, including biochemical, histological and/or behavioral symptoms of a viral infection. Prevention may be in the context of an individual at risk of developing the viral infection, such as where the “at risk” individual does not develop the viral infection over a period of time, such as during a viral season or during a period of exposure to the virus, which may be days to weeks to months. An individual “at risk” of developing a viral infection is an individual with one or more risk factors for developing the viral infection but who has not been diagnosed with and does not display symptoms consistent with a viral infection. Risk factors for developing an RSV infection include but are not limited to an individual’s age (young children under age 5 such as children between about 0 and about 2 years of age, including infants, and individuals greater than 65 years of age), premature birth, co-morbidities associated with RSV and individuals who are immunocompromised.
As used herein, a “therapeutically effective dosage” or “therapeutically effective amount” of compound or salt thereof or pharmaceutical composition is an amount sufficient to produce a desired therapeutic outcome. A therapeutically effective amount or a therapeutically effective dosage can be administered in one or more administrations. A therapeutically effective amount or dosage may be considered in the context of administering one or more therapeutic agents (e.g., a compound, or pharmaceutically acceptable salt thereof), and a single agent may be considered to be given in a therapeutically effective amount if, in conjunction with one or more other agents, a desired therapeutic outcome is achieved. Suitable doses of any of the coadministered compounds may optionally be lowered due to the combined action (e.g., additive or synergistic effects) of the compounds.
As used herein, a “prophylactically effective dosage” or “prophylactically effective amount” is an amount sufficient to effect the preventative result of eliminating or reducing the risk of developing a viral infection or delaying the onset of a viral infection, including biochemical, histological and/or behavioral symptoms of a viral infection. A prophylactically effective amount or a prophylactically effective dosage can be administered in one or more administrations and over a period of time in which such prevention is desired. Additionally, the present invention provides pharmaceutical compositions comprising at least one pharmaceutically acceptable carrier and a therapeutically effective amount of a compound of formula (I).
Also provided are pharmaceutical compositions comprising a pharmaceutically acceptable carrier, a therapeutically active amount of a compound of formula (I), and another antiviral agent, in particular an RSV inhibiting compound.
Also, the combination of another antiviral agent and a compound of formula (I) can be used as a medicine. Thus, the present invention also relates to a product containing (a) a compound of formula (I), and (b) another antiviral compound, as a combined preparation for simultaneous, separate or sequential use in antiviral treatment. The different drugs may be combined in a single preparation together with pharmaceutically acceptable carriers. Other antiviral compounds (b) to be combined with a compound of formula (I) for use in the treatment of RSV are RSV fusion inhibitors or RSV polymerase inhibitors.
In order to prepare the pharmaceutical compositions of this invention, an effective amount of the particular compound, in free base form or acid addition salt form, as the active ingredient is combined in intimate admixture with at least one pharmaceutically acceptable carrier, which carrier may take a wide variety of forms depending on the form of preparation desired for administration. These pharmaceutical compositions are desirably in unitary dosage form suitable, preferably, for oral administration, rectal administration, percutaneous administration, parenteral or intramuscular injection.
For example in preparing the compositions in oral dosage form, any of the usual liquid pharmaceutical carriers may be employed, such as for instance water, glycols, oils, alcohols and the like in the case of oral liquid preparations such as suspensions, syrups, elixirs and solutions; or solid pharmaceutical carriers such as starches, sugars, kaolin, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules and tablets. Because of their easy administration, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharmaceutical carriers are obviously employed. For parenteral injection compositions, the pharmaceutical carrier will mainly comprise sterile water, although other ingredients may be included in order to improve solubility of the active ingredient. Injectable solutions may be prepared for instance by using a pharmaceutical carrier comprising a saline solution, a glucose solution or a mixture of both. Injectable suspensions may also be prepared by using appropriate liquid carriers, suspending agents and the like. In compositions suitable for percutaneous administration, the pharmaceutical carrier may optionally comprise a penetration enhancing agent and/or a suitable wetting agent, optionally combined with minor proportions of suitable additives which do not cause a significant deleterious effect to the skin. Said additives may be selected in order to facilitate administration of the active ingredient to the skin and/or be helpful for preparing the desired compositions. These topical compositions may be administered in various ways, e.g., as a transdermal patch, a spot-on or an ointment. Addition salts of the compounds of formula (I), due to their increased water solubility over the corresponding base form, are obviously more suitable in the preparation of aqueous compositions.
It is especially advantageous to formulate the pharmaceutical compositions of the invention in dosage unit form for ease of administration and uniformity of dosage. “Dosage unit form” as used herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined amount of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Examples of such dosage unit forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, injectable solutions or suspensions, teaspoonfuls, tablespoonfuls and the like, and segregated multiples thereof.
For oral administration, the pharmaceutical compositions of the present invention may take the form of solid dose forms, for example, tablets (both swallowable and chewable forms), capsules or gelcaps, prepared by conventional means with pharmaceutically acceptable excipients and carriers such as binding agents, fillers, lubricants, disintegrating agents, wetting agents and the like. Such tablets may also be coated by methods well known in the art.
Liquid preparations for oral administration may take the form of e.g. solutions, syrups or suspensions, or they may be formulated as a dry product for admixture with water and/or another suitable liquid carrier before use. Such liquid preparations may be prepared by conventional means, optionally with other pharmaceutically acceptable additives such as suspending agents, emulsifying agents, non-aqueous carriers, sweeteners, flavours, masking agents and preservatives.
The compounds of formula (I) may be formulated for parenteral administration by injection, conveniently intravenous, intramuscular or subcutaneous injection, for example by bolus injection or continuous intravenous infusion. Formulations for injection may be presented in unit dosage form, e.g. in ampoules or multi-dose containers, including an added preservative. They may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulating agents such as isotonizing, suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be present in powder form for mixing with a suitable vehicle, e.g. sterile pyrogen free water, before use.
The compounds of formula (I) may also be formulated in rectal compositions such as suppositories or retention enemas, e.g. containing conventional suppository bases such as cocoa butter and/or other glycerides.
In general, it is contemplated that an antivirally effective daily amount would be from 0.01 mg/kg to 500 mg/kg body weight, more preferably from 0.1 mg/kg to 50 mg/kg body weight. It may be appropriate to administer the required dose as two, three, four or more sub-doses at appropriate intervals throughout the day. Said sub-doses may be formulated as unit dosage forms, for example, containing 1 to 1000 mg, and in particular 5 to 200 mg of active ingredient per unit dosage form.
The exact dosage and frequency of administration depends on the particular compound of formula (I) used, the particular condition being treated, the severity of the condition being treated, the age, weight, sex, extent of disorder and general physical condition of the particular patient as well as other medication the individual may be taking, as is well known to those skilled in the art. Furthermore, it is evident that said effective daily amount may be lowered or increased depending on the response of the treated subject and/or depending on the evaluation of the physician prescribing the compounds of the instant invention. The effective daily amount ranges mentioned hereinabove are therefore only guidelines.
1H NMR spectra were recorded on 1) a Bruker Avance DRX 400 spectrometer or Bruker Advance III 400 spectrometer or 2) a Bruker Avance 500 MHz spectrometer and c) Bruker Advance III 400 spectrometer.
NMR spectra were recorded at ambient temperature unless otherwise stated. Data are reported as follow: chemical shift in parts per million (ppm) relative to TMS (δ = 0 ppm) on the scale, integration, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, quin = quintuplet, sex = sextuplet, m = multiplet, b = broad, or a combination of these), coupling constant(s) J in Hertz (Hz).
The High-Performance Liquid Chromatography (HPLC) measurement was performed using a LC pump, a diode-array (DAD) or a UV detector and a column as specified in the respective methods. If necessary, additional detectors were included (see table of methods below).
Flow (expressed in mL/min; column temperature (T) in °C; Run time in minutes) from the column was brought to the Mass Spectrometer (MS) which was configured with an atmospheric pressure ion source. It is within the knowledge of the skilled person to set the tune parameters (e.g. scanning range, dwell time... ) in order to obtain ions allowing the identification of the compound’s nominal monoisotopic molecular weight (MW). Data acquisition was performed with appropriate software.
Compounds are described by their experimental retention times (RT) and ions.
All results were obtained with experimental uncertainties that are commonly associated with the method used.
Hereinafter, “SQD” means Single Quadrupole Detector, “MSD” Mass Selective Detector, “BEH” bridged ethylsiloxane/silica hybrid, “DAD” Diode Array Detector, “HSS” High Strength silica., “Q-Tof” Quadrupole Time-of-flight mass spectrometers, “CLND”, ChemiLuminescent Nitrogen Detector, “ELSD” Evaporative Light Scanning Detector.
Optical rotations were measured on a Perkin Elmer 341 polarimeter and reported as follow [α]λT. λ is the wavelength of light used in nm (if the wavelength of light used is 589 nm, the sodium D line, then the symbol D is used) and T is the temperature in degree Celsius. The sign (+ or -) of the rotation is given. The concentration and the solvent of the sample are provided in brackets after the rotation. The rotation is reported in degrees and no units of concentration are given (it is assumed to be g/100 mL).
The stereochemical configuration for some compounds has been designated as R* or S* (or *R or *S) when the absolute stereochemistry is undetermined (even if the bonds are drawn stereospecifically) although the compound itself has been isolated as a single stereoisomer and is enantiomerically pure. This means that the absolute stereoconfiguration of the stereocentre indicated by * is undetermined (even if the bonds are drawn stereospecifically) although the compound is enantiomerically pure at the indicated centre.
A mixture of 4-amino-3-methoxybenzoate (3.00 g, 18.0 mmol), methacrylaldehyde (3.00 g, 43.0 mmol) and HC1 (12 M aq., 12 mL, 12.0 mmol) was stirred at 100° C. for 5 h. The reaction mixture was cooled in an ice/water bath. The suspension was filtered off. The solid was purified by trituration with EtOAc and petroleum ether (1:30, 10 mL) (twice), to afford 1 (600 mg, 15%) as a gray solid. 1H NMR (400 MHz, DMSO-d6) δ 13.26 (s, 1H), 8.81 (d, J=2.20 Hz, 1H), 8.25 (d, J=0.88 Hz, 1H), 8.12 (d, J=1.54 Hz, 1H), 7.50 (d, J=1.76 Hz, 1H), 4.01 (s, 3H), 2.50 (s, 3H).
SOCl2 (33.6 mL, 460 mmol) was added to a solution of 1 (25.0 g, crude) in CH3OH (300 mL) at 0° C. The reaction mixture was stirred at 80° C. for 40 min. The reaction mixture was concentrated to dryness under reduced pressure. The residue was dissolved in water (100 mL) and the pH of the solution was adjusted to pH 7-8 with NaHCO3 (sat., aq.). The aqueous layer was extracted with EtOAc (3 x 200 mL). The combined organic extracts were washed with brine (200 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 5:1 to 1:2) to afford 2 (10 g, 37 %) as a brown solid.
The reaction was performed on two batches of 5.00 g of 2. m-CPBA (14.0 g, 64.9 mmol, 80% pure) was added to a solution of 2 (5.00 g, 22.0 mmol) in CH2Cl2 (50 mL) at 0° C. The reaction mixture was stirred at rt for 12 h and concentrated to dryness under reduced pressure. The two batches were combined. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 10:1 to 1:1) to afford 3 (5.1 g, 85%) as a brown solid.
A mixture of 3 (3.80 g, 15.4 mmol) and POCl3 (30.0 g, 196 mmol) was stirred at 95° C. for 1 h. The reaction mixture was concentrated to dryness under reduced pressure. The residue was dissolved in water (100 mL) and the aqueous phase was extracted with EtOAc (3 x 30 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 1:0 to 1:1) to afford 4 (2.5 g, 61%) as a white solid.
Tetramethylammonium fluoride (1.80 g, 19.3 mmol) was added to a solution of 4 (2.50 g, 9.41 mmol) in anhydrous DMF (20 mL). The reaction mixture was stirred at rt for 24 h. The reaction mixture was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 1:0 to 1:1) to afford 5 (1.8 g, 68%) as a yellow solid.
LiOH.H2O (612 mg, 14.6 mmol) was added to a solution of 5 (1.8 g, 7.22 mmol) in THF (12 mL) and H2O (6 mL) at 0° C. The resulting reaction mixture was stirred at rt for 2 h. The mixture was combined with another batch (1.30 g, 5.22 mmol), acidified to pH 5 with acetic acid and was concentrated to dryness under reduced pressure. The crude mixture was purified by preparative HPLC (Phenomenex Synergi Max-RP 250 x 50 mm x 10 µm, mobile phase gradient: 5% to 45% (v/v) water (0.225%FA)-CH3CN). The product was suspended in water (50 mL). The mixture was frozen and lyophilized to dryness to give 6 (2.15 g, 72%, 97% pure) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 13.28 (br s, 1H), 8.51 (d, J=10.1 Hz, 1H), 8.17 (d, J=1.3 Hz, 1H), 7.57 (d, J=1.3 Hz, 1H), 4.00 (s, 3H), 2.41 (s, 3H).
NaH (2.00 g, 50.0 mmol, 60% in mineral oil) was added to a solution of cyclopropanol (1.75 g, 30.1 mmol) in THF (15 mL) under N2 atmosphere at 0° C. The mixture was stirred for 1 h and a solution of methyl 3-fluoro-4-nitrobenzoate (5.00 g, 25.1 mmol) in THF (50 mL) was added at 0° C. The reaction mixture was stirred with gradual warming to rt for 16 h. The reaction was quenched with NH4Cl (sat., aq., 30 mL). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 4 mL). The aqueous solution was acidified to pH 5.5 with HCl (1 M) and stirred for 1 h. The solid was collected by filtration, washed with H2O (10 mL) and dried under reduced pressure to afford 7 (4.1 g, 73%) as a yellow solid that was used in the next step without further purification.
A mixture of 7 (2.00 g, 8.96 mmol) and 10% Pd/C (200 mg) in THF (20 mL) was stirred under H2 atmosphere (15 psi) at rt for 16 h. The suspension was filtered through a pad of Celite® and washed with THF (3 x 20 mL). The filtrate was concentrated to dryness under reduced pressure to afford 8 (1.8 g, 97% pure) as a yellow solid.
Methacrylaldehyde (2.70 g, 38.5 mmol) was added to a solution of 8 (2.50 g, 12.9 mmol) in HCl (6 M aq., 30 mL). The reaction mixture was stirred at rt for 30 min, then stirred at 100° C. for 40 min. The reaction mixture was concentrated to dryness under reduced pressure. The crude mixture was purified by preparative HPLC (Phenomenex Synergi Max-RP 250 x 50 mm x 10 µm, mobile phase gradient: 2% to 30% (v/v) CH3CN and H2O with 0.225% HCOOH). The product was suspended in water (30 mL). The mixture was frozen and then lyophilized to dryness to afford 9 (758 mg, 23%, 95% pure) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.78 (d, J=2.2 Hz, 1H), 8.23 (d, J=0.9 Hz, 1H), 8.13 (d, J=1.5 Hz, 1H), 7.85 (d, J=1.8 Hz, 1H), 4.07 (tt, J=2.9, 6.0 Hz, 1H), 2.47 (s, 3H), 0.93 - 0.87 (m, 2H), 0.82 - 0.76 (m, 2H).
2,2,3-Tribromopropanal (18.0 g, 61.1 mmol) was added to a solution of methyl 4-amino-3-methoxybenzoate (10.0 g, 55.2 mmol) in AcOH (120 mL). The reaction mixture was stirred at 100° C. for 1.5 h. The reaction mixture was concentrated to dryness under reduced pressure to afford 10 (18.0 g), which was used in the next step without further purification.
SOCl2 (8.0 mL, 110 mmol) was added to a solution of 10 in CH3OH (150 mL). The reaction mixture was stirred at 80° C. for 1.5 h. The reaction mixture was concentrated to dryness under reduced pressure. The residue was triturated in EtOAc and CH3OH (8:1, 100 mL) and the suspension was isolated via filtration. The filter cake was washed with EtOAc and CH3OH (8:1, 2 x 50 mL) and dried under vacuum to afford 11.
A mixture of 11, potassium trifluoro(prop-1-en-2-yl)borate (7.60 g, 56.7 mmol), and K3PO4 (21.6 g, 102 mmol) in 1,4-dioxane (125 mL) and H2O (25 mL) was purged with N2 for 5 min. Pd(dtbpf)Cl2 (3.30 mg, 5.06 mmol) was added and the mixture was purged with N2 for another 5 min. The reaction mixture was stirred at 100° C. for 1 h. The mixture was cooled to rt and the reaction was quenched with water (80 mL). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 120 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by column chromatography (petroleum ether/EtOAc, gradient from 12:1 to 1:2) to afford 12 (5.0 g, 33% over 3 steps) as a yellow solid.
K2OSO4•2H2O (310 mg, 0.84 mmol) was added to a solution of 12 (5.0 g, 21 mmol) in 1,4-dioxane (50 mL) and H2O (50 mL). NaIO4 (14.0 g, 65.4 mmol) was added. The reaction mixture was stirred at rt for 2 h. The reaction was poured into water (50 mL), and the aqueous phase was extracted with EtOAc (3 x 80 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by column chromatography (petroleum ether/EtOAc, gradient from 10:1 to 1:2) to afford 13 (4.0 g, 79%) as a light yellow solid.
DAST (10.0 mL, 75.7 mmol) was added dropwise to a solution of 13 (4.00 g, 16.3 mmol) in CH2Cl2 (50 mL) at 0° C. under N2 atmosphere. The reaction mixture was stirred at 0° C. for 2 h. The reaction mixture was diluted with CH2Cl2 (20 mL) and poured into NaHC03 (sat., aq., 50 mL). The layers were separated, and the aqueous phase was extracted with CH2Cl2 (2 x 50 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 10:1 to 2:3) to afford 14 (2.1 g, 46%, 95% pure) as a yellow solid.
NaOH (630 mg, 15.7 mmol) was added to a solution of 14 (2.10 g, 7.86 mmol) in CH3OH (20 mL) and H2O (4 mL). The reaction mixture was stirred at rt for 3 h and diluted with H2O (25 mL) and CH2Cl2 (30 mL). The layers were separated, and the aqueous phase was diluted with HCl (1N) until pH 6. The suspension was isolated via filtration and washed with H2O (3 x 15 mL) and dried under vacuum. The residue was suspended in H2O (20 mL) and the mixture was frozen and lyophilized to dryness to afford 15 (1.71 g, 85%) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 9.10 (s, 1H), 8.80 (s, 1H), 8.35 (s, 1H), 7.66 (s, 1H), 7.52 - 7.19 (m, 1H), 4.04 (s, 3H).
11 (3.00 g, 10.1 mmol), cyclopropylboronic acid (990 mg, 11.5 mmol) and CS2CO3 (4.95 g, 15.2 mmol) were dissolved in toluene (40 mL) and H2O (2 mL). The mixture was purged with Ar for 5 min and Pd2(dba)3 (4.62 g, 5.05 mmol) and XPhos (480 mg, 1.01 mmol) were added. The reaction mixture was purged with Ar for another 5 min and stirred at 115° C. for 2 h. The mixture was cooled to rt and the reaction was quenched with water (40 mL). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 60 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 12:1 to 4:1) to afford 16 (2.5 g, 71%) as a yellow solid.
NaOH (665 mg, 16.6 mmol) was added to a solution of 16 (2.00 g, 7.77 mmol) in CH3OH (20 mL) and H2O (4 mL). The reaction mixture was stirred at rt for 3 h. The mixture was diluted with water (30 mL) and CH2Cl2 (20 mL). The layers were separated, and the pH of the aqueous layer was adjusted to 6 with HCl (1N, aq.). The suspension was isolated by filtration and washed with water (3 x 20 mL) and dried under reduced pressure. The residue was suspended in water (30 mL) and the mixture was frozen then lyophilized to dryness to afford 17 (1.3 g, 67%) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.79 (d, J=2.2 Hz, 1H), 8.11 (d, J=1.3 Hz, 1H), 8.07 (d, J=2.0 Hz, 1H), 7.47 (d, J=1.1 Hz, 1H), 3.99 (s, 3H), 2.19 - 2.11 (m, 1H), 1.13 -1.06 (m, 2H), 0.92 - 0.86 (m, 2H).
A solution of methyl 4-amino-3-iodo-5-methoxybenzoate (45.0 g, 146 mmol) and HCl (6 M in H2O, 180 mL, 1.08 mol) was cooled in an ice bath (0° C.) while a solution of NaNO2 (12.5 g, 181 mmol) in cold water (50 mL) was added dropwise. The resulting solution of diazonium salt was stirred at 0° C. for 30 min and was added to a solution of diethylamine (39 mL, 377 mmol) and K2CO3 (102 g, 734 mmol) in CH3CN and H2O (1:2, 390 mL) dropwise. The reaction mixture was stirred at 0° C. for 30 min. The reaction mixture was extracted with CH2Cl2 (2 x 500 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 10:1 to 8:1) to afford 18 (53 g, 84%, 91% pure) as a yellow oil.
The reaction was performed on two batches of 25 g of 18. 18 (25 g, 63.9 mmol), trimethyl(prop-1-yn-1-yl)silane (57.5 g, 512 mmol), CuI (2.5 g, 13.1 mmol) and CsF (49.0 g, 323 mmol) were dissolved in DMF (250 mL) and CH3OH (50 mL). The mixture was purged with Ar for 5 min and Pd(PPh3)2Cl2 (2.25 g, 3.21 mmol) was added. The mixture was purged with Ar for another 5 minutes and the reaction mixture was stirred at rt for 2 h. The two batches were combined and poured into a brine (500 mL). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 500 mL). The combined organic extracts were washed with brine (50 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, 15:1) to afford 19 (36 g, 88%, 95% pure) as a yellow oil.
HBr (48% aq., 54 mL, 477 mmol) was added to a solution of 19 (18.0 g, 59.3 mmol) in acetone (200 mL) at 0° C. The reaction mixture was stirred for 1.5 h with gradual warming to rt. Acetone was evaporated under reduced pressure and the residue was dissolved in CH2Cl2 (250 mL). The solution was washed with NaHCO3 (sat., aq., 150 mL). The aqueous layer was extracted with CH2Cl2 (2 x 250 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude product 20 (18 g, 88%, 90% pure) was used in the next step without further purification.
10% Pd/C (5.0 g) was added to a solution of 20 (20.0 g, 64.3 mmol) in CH3OH (300 mL). The reaction mixture was stirred under H2 atmosphere (15 psi) at rt for 1.5 h. The reaction mixture was filtered through a pad of Celite® and washed with CH3OH (300 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude 21 (13 g) was used in the next step without further purification.
MnO2 (14.5 g, 167 mmol) was added to a solution of 21 (13 g, crude) in CH2Cl2 (130 mL). The reaction mixture was stirred at 40° C. for 1.5 h. The reaction mixture was filtered through a pad of Celite® and washed with CH2Cl2 (300 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 10:1 to 0:1) to afford 22 (4.2 g, 25% over 2 steps, 91% pure) as a yellow solid.
NaOH (1.05 g, 26.3 mmol) was added to a solution of 22 (3.80 g, 16.4 mmol) in CH3OH (30 mL) and H2O (6 mL). The reaction mixture was stirred at rt for 1 h. CH3OH was evaporated under reduced pressure and the aqueous phase was washed with CH2Cl2 (30 mL). The pH of the solution was adjusted to pH 2 with HCl (2 M aq.). The suspension was isolated by filtration, the solid was washed with water (20 mL) and the solvent removed under reduced pressure. The product was triturated in EtOAc (10 mL), isolated by filtration and washed with EtOAc (10 mL) before being dried under reduced pressure to afford 23 (3.02 g, 85%) as a brown solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 13.59 (br. s., 1H), 8.17 - 8.11 (m, 2H), 7.57 - 7.53 (m, 1H), 4.12 (s, 3H), 2.88 (s, 3H).
To a mixture of methyl 7-methoxy-2-methyl-1H-indole-5-carboxylate (10.0 g, 45.6 mmol) and t-BuOK (7.68 g, 68.4 mmol) in DMF (150 mL) was added O-(4-nitrobenzoyl)hydroxylamine (12.5 g, 68.4 mmol). The reaction mixture was stirred at rt for 2 h. The reaction was quenched with NH4Cl (sat., aq.) and diluted with EtOAc (300 mL). The layers were separated and the organic phase was washed with water (5 x 300 mL) and brine (3 times). The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 80:20) to afford 24 (6.6 g, 62%).
To a solution of 24 (700 mg, 3.00 mmol) in CH3OH (50 mL) was added HCl (0.90 mmol). The reaction mixture was stirred at 90° C. for 24 h. The mixture was cooled to rt, filtered and concentrated under reduced pressure. The crude was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 50:50) to afford 25 (0.3 g, 43%) as a yellow solid.
To a solution of 25 (1.90 g, 5.04 mmol) in CH2Cl2 (10 mL) and CH3CN (10 mL) was added 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (5.94 g, 26.2 mmol). The reaction mixture was stirred at rt for 3 h. The mixture was filtered and concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 50:50) to afford 26 (2.9 g, 73%) as a yellow solid.
To a solution of 26 (900 mg, 3.88 mmol) in CH2Cl2 (50 mL) was added BBr3 (50 mL, 50 mmol). The reaction mixture was stirred at rt for 3 h, filtered and concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 50:50) to afford 27 (2.9 g, 73%) as a yellow solid.
To a solution of 27 (800 mg, 3.67 mmol) in DMF (50 mL) were added sodium chlorodifluoroacetate (1.12 g, 7.33 mmol) and CS2CO3 (3.58 g. 11.0 mmol). The reaction mixture was stirred at rt for 12 h. The mixture was filtered and concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 50:50) to afford 28 (250 mg, 25%) as a yellow solid.
To a solution of 28 (170 mg, 0.63 mmol) in CH3OH (5 mL), THF (5 mL) and H2O (1 mL) was added NaOH (101 mg, 2.54 mmol). The reaction mixture was stirred at rt for 2 h. The reaction was neutralized with HCl (1N, aq., 4 mL) and the aqueous phase was extracted with EtOAc (3 x 20 mL). The combined organic extracts were washed with brine, dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by C-18 column chromatography (5-60% CH3CN/H2O (0.05% HCl) to deliver 29 (109 mg, 66%) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.52 (d, J=1.2 Hz, 1H), 8.32 (s, 1H), 7.90 - 7.51 (m, 1H), 2.93 (s, 3H).
To a mixture of CH3OH (75 mL) and water (8 mL) was added 4-bromo-2-(trifluoromethoxy)-aniline 5.00 g, 19.5 mmol). The mixture was cooled in an ice bath and concentrated H2SO4 (3 mL) was added followed by ICl (3.17 g, 19.5 mmol) in CH2Cl2 (19.5 mL). The reaction mixture was stirred at rt for 10 h. The reaction was quenched Na2S2O3 (sat., aq.). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 100 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 30 (3.75 g, 50%) as a yellow solid.
30 (3.70 g, 9.69 mmol), CuI (369 mg, 1.94 mmol) and CsF (4.42 g, 29.1 mmol) were dissolved in DMF (120 mL) and CH3OH (30 mL). The mixture was cooled to 0° C. and trimethyl(prop-1-yn-1-yl)silane (2.18 g, 19.4 mmol) was added. The mixture was purged with N2 and PdCl2(PPh3)2 (0.34 g, 0.48 mmol) was added. The reaction mixture was purged again with N2 and stirred at rt for 1 h. The reaction was quenched with NH4Cl (sat., aq.). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 200 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 31 (2.5 g, 88%) as a yellow solid.
31 (2.30 g, 7.82 mmol) was dissolved in NMP (50 mL). The mixture was purged with N2 and t-BuOK (2.19 g, 19.5 mmol) was added. The mixture was purged again with N2 and stirred at rt for 2 h. The reaction was quenched with NH4Cl (sat., aq.). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 200 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 32 (1.95 g, 85%) as a yellow solid.
32 (1.90 g, 6.46 mmol) was dissolved in DMF (50 mL). The mixture was purged with N2 and t-BuOK (1.09 g, 9.69 mmol) was added followed by O-(4-nitrobenzoyl)hydroxylamine (1.76 g, 9.69 mmol). The reaction mixture was purged again with N2 and stirred at rt for 2 h. The reaction was quenched with NH4Cl (sat., aq.). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 200 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 80:20) to afford 33 (1.74 g, 87%) as a yellow solid.
To a solution of 33 (400 mg, 1.29 mmol) in CH3OH (30 mL) was added HCl (14.2 mg, 0.39 mmol). The reaction mixture was stirred at 90° C. for 24 h. The mixture was cooled to rt. The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 34 (90 mg, 23%) as a yellow solid.
To a solution of 34 (2.00 g, 6.51 mmol) in CH3OH (50 mL) were added Pd(dppf)Cl2.CH2Cl2 (0.55 g, 0.65 mmol) and Et3N (1.32 g, 13.0 mmol). The reaction mixture was purged with CO (1 atm) and stirred at 50° C. for 10 h. The mixture was cooled to rt, filtered and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (CH2Cl2/EtOAc, gradient from 100:0 to 95:5) to afford 35 (853 mg, 46%) as a yellow solid.
To a solution of 35 (923 mg, 3.23 mmol) in CH3OH (15 mL) and THF (15 mL) was added NaOH (387 mg, 9.68 mmol) and H2O (1 mL). The reaction mixture was stirred at rt for 30 min. The mixture was filtered, and the filtrate was concentrated under reduced pressure. The residue was purified by reverse phase chromatography to afford 36 (562 mg, 64%) as a yellow solid. 1H NMR (300 MHz, DMSO-d6) δ ppm 8.69 (d, J=1.6 Hz, 1H), 8.37 (s, 1H), 8.14 - 8.07 (m, 1H), 2.94 (s, 3H).
To a mixture of 4-bromo-2-cyclopropoxy-1-nitrobenzene (350 g, 1.59 mol) and cyclopropanol (166 g, 2.86 mol) in 2-MeTHF (3.5 L) was added NaH (60% pure, 114 g, 2.86 mol) at 0° C. The reaction mixture was stirred at rt for 16 h under N2 atmosphere. The reaction was quenched with NH4Cl (sat., aq., 2.5 L). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 1 L). The combined organic extracts were washed with brine (2 L), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 37 that was used without further purfication in the next step.
To a solution of 37 (410 g, 1.59 mol) in AcOH (909 mL, 15.9 mol) in THF (2.5 L) was added Fe (444 g, 7.94 mol). The reaction mixture was stirred at 60° C. for 2 h. The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 90:10 to 85:15) to afford 38 (320.5 g, 84% over 2 steps, 96% pure) as yellow oil.
A mixture of 38 (320 g, 1.41 mol) and Boc2O (368 g, 1.69 mol) in CH3OH (3 L) was stirred at 75° C. for 16 h. The reaction mixture was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, 20:1) to afford 39 (420.5 g, 91% yield) as light yellow solid.
Three reactions were carried out in parallel. To a mixture of 39 (110 g, 335 mmol), Et3N (136 g, 1.34 mol), and dppf (18.6 g, 33.5 mmol) in CH3OH (600 mL) and DMF (300 mL) was added Pd(OAc)2 (3.76 g, 16.7 mmol). The reaction mixture was stirred at 80° C. for 16 h under CO atmosphere (50 psi). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was diluted with H2O (4 L) and extracted with EtOAc (3 x 1.5 L). The combined organic extracts were washed with brine (2 L) and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 40 (210.5 g, 65%, 96% pure) as light yellow oil.
To a solution of 40 (211 g, 685 mmol) in CH2Cl2 (1.6 L) was added TFA (588.1 g, 5.16 mol) and the reaction mixture was stirred at rt for 16 h. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in EtOAc (1 L) and NaHCOs (aq., 1 L) was added. The layers were separated, and the aqueous phase was extracted with EtOAc (2 x 500 mL). The combined organic extracts were washed with brine (1 L) and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 41 (140.5 g, 97%, 98% pure) as yellow oil.
To a solution of 41 (140 g, 676 mmol) and NaHCO3 (116 g, 1.38 mol) in CH2Cl2 (1 L) was added ICl (121 g, 743 mmol) in CH2Cl2 (200 mL). The reaction mixture was stirred at rt for 1.5 h. The reaction was quenched with Na2SO3 (sat., aq., 1 L). The layers were separated, and the aqueous phase was extracted with CH2Cl2 (2 x 500 mL). The combined organic extracts were washed with brine (500 mL) and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 42 (103.5 g, 46%) as yellow solid.
To a solution of 42 (103 g, 310 mmol) in HCl (6 M, 259 mL) in CH3CN (130 mL) was added a solution of sodium nitrite (32.1 g, 466 mmol) in H2O (65 mL) at 0° C. The reaction mixture was stirred at 0° C. for 30 min to afford the corresponding diazonium salt. To a suspension of N-ethylethanamine (45. 5 g, 621 mmol) and K2CO3 (215 g, 1.55 mol) in CH3CN (250 mL) and H2O (500 mL) was added the freshly prepared diazonium salt at 0 - 5° C. The reaction mixture was stirred at 0° C. for 1 h. The reaction mixture was diluted with H2O (500 mL) and extracted with EtOAc (3 x 500 mL). The combined organic extracts were washed with brine (500 mL) and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 43 (114.5 g, 83%) as yellow oil.
To a suspension of 43 (114 g, 273 mmol), CsF (207 g, 1.37 mol), CuI (10.4 g, 54.6 mmol) and Pd(PPh3)2Cl2 (9.59 g, 13.7 mmol) in DMF (1 L) and CH3OH (200 mL) was added trimethyl(prop-1-ynyl)silane (61.3 g, 546 mmol) under N2 atmosphere. The reaction mixture was stirred at rt for 16 h under N2. The reaction mixture was filtered, and the filtrate was diluted with H2O (4 L). The aqueous phase was extracted with EtOAc (3 x 1.5 L). The combined organic layers were washed with brine (2 L) and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 90:10 to 85:15) to afford 44 (89.3 g, 99%) as a yellow oil.
Three reactions were carried out in parallel. To a solution of 44 (89.2 g, 271 mmol) in acetone (550 mL) was added HBr (137 g, 812 mmol, 48% pure) at 0 - 10° C. The reaction mixture was stirred at 0° C. for 1.5 h. The solids were isolated by filtration. The filter cake was dissolved in CH2Cl2 (1.5 L) and Et3N (75 mL), washed with H2O (500 mL), brine (500 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 45 (83.5 g, 246 mmol, 91%) as a yellow solid.
Two reactions were carried out in parallel. A suspension of 45 (41.5 g, 123 mmol) and wet 10% Pd/C (6.10 g) in CH3OH (600 mL) was stirred at rt for 2.5 h under H2 atmosphere. The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (CH2Cl2/Et2O) to afford 46 (24.5 g, 36%, 95% pure) as a yellow solid.
A suspension of 46 (24.5 g, 94.1 mmol) and MnO2 (40.9 g, 471 mmol) in CH2Cl2 (300 mL) was stirred at rt for 16 h. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure to afford 47 (23.5 g, 97%) as a yellow solid, which was used directly in the next step without purification.
To a solution of 47 (23.5 g, 90.9 mmol) in CH3OH (200 mL) and H2O (100 mL) was added KOH (6.13 g, 109 mmol). The reaction mixture was stirred at rt for 1.5 h. To the reaction mixture was added TFA until pH ≈ 2. Precipitation occurred. The solids were isolated by filtration to afford 48 (16.5 g, 74%). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.16 (d, J=1.54 Hz, 1H), 8.14 (s, 1H), 7.90 (d, J=1.54 Hz, 1H), 4.17-4.26 (m, 1H), 2.88 (s, 3H), 0.92-1.01 (m, 2H), 0.84-0.92 (m, 2H); LC-MS (method D): Rt = 1.07 min; mass calcd. for C13H12N2O3 244.0, m/z found 245.0 [M+H]+.
To a cooled solution of 4-bromo-2-fluoroaniline (7.60 g, 40 mmol) in H2O (60 mL) was added –HCl (conc., 10 mL) and NaNO2 (3.31 g, 48.0 mmol). After 20 min at 0° C., conc. HCl (13 mL) and NaBF4 (17.6 g, 160 mmol) were added. The mixture was stirred for 40 min and the diazonium salt was isolated by filtration, washed with H2O and Et2O. A solution of diazonium salt in CH3CN (60 mL) was treated with ethyl 3-morpholinoacrylate (3.26 g, 17.6 mmol). The reaction mixture was stirred at rt for 16 h. The solvent was removed under reduced pressure and the residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 80:20) to afford 49 (4.5 g, 35%) as a yellow solid.
A mixture of 49 (7.50 g, 23.7 mmol) in concentrated sulfuric acid (100 mL) was heated at 100° C. for 3 h. The mixture was cooled to 0° C., and the mixture was diluted with H2O (200 mL). The brown solid was removed by filtration, and the filtrate was extracted with CH2Cl2 (4 x 600 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure to afford 50 (2.5 g, 39%) as a dark solid.
To a solution of 50 (13.0 g, 48.0 mmol) in DMF (150 mL) were added N,O-dimethylhydroxylamine hydrochloride (6.09 g, 62.4 mmol), HATU (21.9 g, 57.6 mmol) and DIPEA (18.6 g, 144 mmol). The reaction mixture was stirred at rt for 6 h. The reaction was quenched with H2O (100 mL) and extracted with EtOAc (3 x 200 mL). The combined organic layers were washed with H2O (2 x 300 mL) and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 50:50) to afford 51 (7.0 g, 46%) as a yellow solid.
To a solution of 51 (7.00 g, 22.3 mmol) in THF (6 mL) at -78° C. was added LiAlH4 (3.38 g, 89.1 mmol). The mixture was stirred at -78° C. for 1 h. The mixture was warmed to rt and the reaction was quenched with Na2SO4.10 H2O (10 g). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure to afford 52 (5.4 g, 94%) as a yellow solid.
To a solution of 52 (5.40 g, 21.0 mmol) at 0° C. in CH2Cl2 (30 mL) was added DMP (13.4 g, 31.5 mmol). The reaction mixture was stirred at rt for 12 h. The solvent was removed under reduced pressure and the crude mixture was purified by silica column chromatography (CH2Cl2/EtOAc, gradient from 100:0 to 95:5) to afford 53 (2.5 g, 47%) as a yellow solid.
To a solution of 53 (408.1 mg, 1.6 mmol) at -20° C. in CH2Cl2 (10 mL) was added DAST (1.03 g, 6.40 mmol). The reaction mixture was stirred at rt for 12 h. The reaction was quenched with H2O (10 mL). The layers were separated, and the aqueous phase was extracted with CH2Cl2 (3 x 20 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 70:30) to afford 54 (400 mg, 90%) as a yellow solid.
To a solution of 54 (2.32 g, 8.37 mmol) in CH3OH (60 mL) was added NaOMe (1.81 g, 33.5 mmol). The reaction mixture was stirred at rt for 6 h. The reaction was quenched with H2O (15 mL) and extracted with EtOAc (3 x 80 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford a yellow solid (2 g).
To a solution of the residue in CH3OH (100 mL) were added Pd(dppf)Cl2•CH2Cl2 (565 mg, 0.69 mmol) and Et3N (1.40 g, 13.8 mmol). The reaction mixture was stirred under CO atmosphere (1 atm) at 50° C. for 1 h. The reaction mixture was cooled to rt and the solvent was removed under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 70:30) to afford 55 (1.8 g, 80%) as a yellow solid.
To a solution of 55 (1.20 g, 4.47 mmol) in CH3OH (40 mL) were added NaOH (536 mg, 13.4 mmol) and H2O (4 mL). The reaction mixture was stirred at rt for 6 h. The reaction was neutralized with IN HCl (20 mL). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 60 mL). The combined organic extracts were washed with brine and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by C-18 column chromatography (CH3CN/H2O (0.05% TFA), gradient from 85:15 to 60:40) to afford 56 (1.02 g, 89%) as a yellow solid. 1H NMR (300 MHz, DMSO-d6) δ ppm 13.77 (brs, 1H), 8.77 (s, 1H), 8.40 (d, J=1.5 Hz, 1H), 7.75 (d, J=1.5 Hz, 1H), 7.59 (t, J=54.0 Hz, 1H), 4.18 (s, 3H).
Methyl 4-amino-3-iodo-5-methoxybenzoate (3.07 g, 10.0 mmol), Pd(PPh3)2Cl2 (702 mg, 1.00 mmol) and CuI (381 mg, 2.00 mmol) were dissolved in THF (50 mL). The reaction mixture was purged with N2. Then ethynylcyclopropane (1.69 mL, 20.0 mmol) was added at 0° C., followed by diisopropylamine (4.20 mL, 30.0 mmol). The mixture was purged again with N2 and the reaction mixture was stirred at 0° C. for 2 h. NH4Cl (sat., aq.) was added. The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 100 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 57 (2.27 g, 94%) as a yellow solid.
57 (2.60 g, 10.6 mmol) was dissolved in NMP (50 mL). The mixture was purged with N2 and t-BuOK (2.97 g, 26.5 mmol) was added. The mixture was purged again with N2 and the reaction mixture was stirred at rt for 2 h. NH4Cl (sat., aq.) was added. The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 100 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 80:20) to afford 58 (1.75 g, 67%) as a yellow solid.
58 (1.75 g, 7.14 mmol) was dissolved in DMF (40 mL). The mixture was purged with N2 and t-BuOK (1.20 g, 10.7 mmol) was added followed by O-(4-nitrobenzoyl)hydroxylamine (1.95 g, 10.7 mmol). The mixture was purged again with N2 and the reaction mixture was stirred at rt for 2 h. NH4Cl (sat., aq.) was added. The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 100 mL). The combined organic extracts were dried (Na2SO4), the solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 80:20) to afford 59 (0.8 g, 43%) as a yellow solid.
To a solution of 59 (650 mg, 2.50 mmol) in CH3OH (40 mL) was added HCl (0.75 mmol). The reaction mixture was stirred at 90° C. for 24 h. The mixture was cooled to rt, filtered and concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 50:50) to afford 60 (430 mg, 62%) as a yellow solid.
To a solution of 60 (430 mg, 1.67 mmol) in CH3OH (5 mL), THF (5 mL) and H2O (0.5 mL) was added NaOH (266 mg, 6.66 mmol) and the reaction mixture was stirred at rt for 2 h. The reaction was neutralized with HCl (1N, aq., 4 mL). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 20 mL). The combined organic layers were washed with brine and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by C-18 column chromatography (CH3CN/H2O (0.05% HCl), gradient from 95:5 to 60:40) to give 61 (138.5 mg, 33%) as a yellow solid. 1H NMR (300 MHz, DMSO-d6) δ ppm 8.13 - 8.12 (m, 2H), 7.53 -7.52 (d, J= 1.2 Hz, 1H), 4.11 (s, 3H), 2.50 (s, 1H), 1.26 - 1.16 (m, 4H).
To a solution of methyl 3-amino-2,6-dibromopyridine-4-carboxylate (30.1 g, 96.9 mmol) in toluene (250 mL) and CH3OH (80 mL) under N2 atmosphere were added 4-fluorophenylboronic acid (12.9 g, 92.3 mmol), Na2CO3 (23.7 g, 223 mmol) and Pd(PPh3)4 (5.60 g, 4.85 mmol). The reaction mixture was stirred at 80° C. for 20 h in a closed reactor. The catalyst was removed by filtration over Celite®. The filtrate was extracted with EtOAc, washed with water (twice) and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was combined with other fractions (96.9 mmol and 96.7 mmol). The crude mixture was purified by silica column chromatography (heptane/CH2Cl2, 50:50) to afford 62 (58.5 g, 65%).
To a solution of 62 (14.2 g, 43.7 mmol) in HCl (conc., 60 mL) was added a solution of sodium nitrite (7.54 g, 109 mmol) in H2O (15 mL) dropwise at 0° C. The mixture was stirred for 30 min and a suspension of CuCl (15.1 g, 153 mmol) in HCl (conc., 30 mL) was added slowly. Following the addition, HCl (6 M aq., 90 mL) was added in portions and the suspension was stirred at 65° C. for 1.5 h. The reaction mixture was cooled to rt and diluted with EtOAc and water. NH3 in H2O was added until pH 7-8. The layers were separated, and the aqueous phase was extracted with EtOAc (twice), and dried (MgSOi). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/CH2Cl2, 1:1) to afford 63 (11.2 g, 86%) as a white solid.
To a solution of 63 (11.0 g, 36.7 mmol) in THF (230 mL) was added CH3MgBr (3.4 M in 2-MeTHF, 24.8 mL, 84.4 mmol) dropwise at -20° C. The reaction mixture was warmed to rt and stirred for 1.5 h. Additional amount of CH3MgBr (3.4 M in 2-MeTHF, 11.8 mL, 40.4 mmol) was added at -20° C. The reaction was quenched with cold NH4Cl (sat., aq.). The layers were separated, and the aqueous phase was extracted with EtOAc, washed with brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The residue was dissolved in a solution of heptane and EtOAc (80:20) and purified by silica column chromatography (heptane/EtOAC, gradient from 100:0 to 80:20) to afford 64 (5.18 g, 47%) as a white solid.
To a solution of 64 (7.97 g, 26.5 mmol) in 1,4-dioxane (120 mL) were added a solution of CS2CO3 (24.3 g, 74.7 mmol) in water (12 mL), 4,4,5,5-tetramethyl-2-(prop-1-en-2-yl)-1,3,2-dioxaborolane (7.33 g, 43.6 mmol) and PdCl2(dppf).CH2Cl2 (2.26 g, 2.77 mmol) under N2 atmosphere. The reaction mixture was stirred at 90° C. for 4 h in a closed reactor. The reaction mixture was cooled to rt and mixed with another fraction (1.65 mmol). The mixture was diluted with EtOAc and filtered over Celite®. The filtrate was extracted with EtOAc, washed with water and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2) to afford 65 (7.05 g, 82%) as a yellowish solid.
To a solution of 65 (4.70 g, 15.4 mmol) and N-boc-O-tosylhydroxylamine (6.63 g, 23.1 mmol) in t-BuOH (65 mL), CH3CN (28 mL) and water (28.2 mL) was added K2OSO4•2H2O (1.13 g, 3.07 mmol). The reaction mixture was stirred at rt for 7 h. The mixture was combined with another fraction (8.17 mmol) and the mixture was extracted with EtOAc, washed with water (twice) and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 90:10) to afford 66 (5.87 g, 57%) as an orange oil.
To a solution of 66 (5.80 g, 13.2 mmol) in CH2Cl2 (80 mL) was added TFA (10.0 mL, 131 mmol). The reaction mixture was stirred at rt for 2 h and concentrated under reduced pressure. The residue was diluted with CH2Cl2 and the solution was washed with NaHCO3. The product precipitated and was collected by filtration to afford a mixture of enantiomers that were purified by silica column chromatography (CH2Cl2/7M NH3 in CH3OH) (4.13 g, 92%); 1H NMR (400 MHz, DMSO-d6) δ ppm 8.13 (s, 1H), 7.58 - 7.68 (m, 2H), 7.27 (m, 2H), 5.56 (m, 1H), 5.56 (s, 1H), 5.20 (s, 1H), 3.09 - 3.27 (m, 2H), 1.63 (s, 6 H), 1.62, 1.36 (s, 3H); LC-MS (method D): Rt = 2.08 min, mass calcd. for C17H20ClN2O2 m/z 338.1, found 339.2 [M+H]+. The enantiomers were separated by SFC (stationary phase: Daicel Chiralcel-IC 5 mu 300 g, mobile phase: 80% CO2, 20% CH3OH + 1% i-PrNH2) to afford 67 (1.6 g, 41%) [α]D20 -23.47 (c 0.375, DMF); and 68 (1.6 g, 41%) [α]D20 +25.51 (c 0.345, DMF).
A mixture of methyl 2-chloro-3-fluoro-4-pyidine carboxylate (23.6 g, 124 mmol), 4-fluoro-phenylboronic acid (34.8 g, 249 mmol) and K3PO4 (79.3 g, 373 mmol) in 2-MeTHF (1.4 L) and H2O (292 mL) was purged with N2. XPhos Pd G2 (7.70 g, 9.79 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 80° C. for 4 h. The reaction mixture was diluted with EtOAc and water. The layers were separated, and the organic phase was dried (MgSO4). The solids were removed by filtration and the solvent of the filtrate was removed under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 9:1 to 1:1) to afford 69 (28.5 g, 92%) as an off-white solid.
To a solution of 69 (4.00 g, 16.1 mmol) in anhydrous CH2Cl2 (160 mL) at 0° C. was added m-CPBA (14.8 g, 64.2 mmol, 75% pure). The mixture was warmed to rt and stirred for 2 days. The reaction was quenched with NaOH (1N, aq.). The organic phase was successively washed with NH4Cl (sat., aq.), Na2S2O3 (10%, aq.), water and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 90:10) to afford 70 (3.21 g, 75%) as a pale-yellow solid.
In a sealed tube, a solution of 70 (3.21 g, 12.1 mmol) in POCl3 (51 mL) was stirred at 80° C. for 20 h. The reaction mixture was concentrated under reduced pressure. The brown residue was taken up in water and EtOAc and the mixture was basified with K2CO3 powder. The layers were separated and the aqueous phase was extracted with EtOAc (twice). The combined organic layers were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure to afford 71 (3.27 g, 95%) as a brown solid.
The reaction was performed on 3 batches of 38.5 g. To a solution of 71 (38.5 g, 136 mmol) in THF (550 mL) was added CH3MgBr (3.4 M in 2-MeTHF, 100 mL, 340 mmol) dropwise at -50° C. The mixture was stirred at rt for 2 hours, then cooled to -50° C. and treated with a NH4Cl solution. The mixture was diluted with water and extracted with EtOAc (twice). The combined organic layers were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was triturated in water. The solid was collected by filtration and dried at 50-60° C. to afford 72 (113.3 g, 99%) as a white solid.
A microwave vial equipped with a magnetic stir bar was charged with tributyl(1-ethoxyvinyl)tin (6.75 mL, 19.4 mmol) and 72 (5.00 g, 17.6 mmol) in 1,4-dioxane (15 mL). PdCl2(PPh3)2 (1.24 g, 1.76 mmol) was added and the reaction mixture was stirred at 100° C. for 6 h. The reaction mixture was filtered through Celite® and washed with 1,4-dioxane (3 times). The filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 0:100) to afford 73 (1.68 g, 30%).
To a solution of 73 (1.68 g, 5.26 mmol) in THF (14 mL) and water (4 mL) at 0° C. was added NBS (0.94 g, 5.26 mmol). The reaction mixture was warmed to rt and stirred for 5 h. The mixture was diluted with water and the aqueous phase was extracted with EtOAc (3 x 200 mL). The combined organic extracts were washed with NaHCO3 (sat., aq.) and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure to afford 74 that was used in the next step without further purification.
74 (11.0 g, 29.7 mmol) was added to a suspension of K2CO3 (4.52 g, 32.7 mmol) and dibenzylamine (6 mL, 31 mmol) in DMF (40 mL). The reaction mixture was stirred at rt for 2 h. The mixture was diluted with water (600 mL) and the aqueous phase was extracted with EtOAc (4 x 200 mL). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was passed through packed alumina. The solvent of the filtrate was removed under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc) to afford 75 (13.8 g, 95%).
To a solution of 75 (6.68 g, 13.7 mmol) in anhydrous THF (125 mL) at 0° C. was added i-PrMgBr (2.9 M in 2-MeTHF, 23.7 mL, 68.6 mmol). The reaction mixture was stirred at rt for 2 h. CH3OH (40 mL) and HCl (4 M, aq., 6 mL) were added and the mixture was stirred for 30 min. The solvent was removed under reduced pressure. NaHCO3 (sat., aq.) was added and the aqueous phase was extracted with EtOAc (3 times). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 0:100). Heptane was added to the residue and a precipitated was formed. The precipitate was collected by filtration to give a first crop of 76 (390 mg, 5%). The filtrate was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 40:60) to afford a second crop of 76 (3.41 g, 43%, 91% pure).
A solution of 76 (4.2 g, 7.92 mmol) in CH3OH (150 mL) was purged with N2 and Pd/C (10%, 842 mg) was added. The flask was sealed and exposed to H2 atmosphere. The reaction mixture was stirred at rt for 4 h. The mixture was filtered through packed Celite® and the solvent was removed under reduced pressure. 1H NMR (400 MHz, DMSO-d6) δ ppm 7.90 - 7.97 (m, 2 H), 7.87 (d, J=5.7 Hz, 1 H), 7.29 - 7.37 (m, 2 H), 5.54 (s, 1 H), 4.72 - 5.14 (m, 1 H), 3.16 (d, J=12.8 Hz, 2 H), 2.83 (d, J=12.8 Hz, 1 H), 2.15 (spt, J=6.8 Hz, 1 H), 1.54 (s, 6 H), 0.87 (d, J=6.8 Hz, 3 H), 0.67 (d, J=6.8 Hz, 3 H). The enantiomers were separated by SFC (stationary phase: Chiralpak Diacel AD 20 x 250 mm, mobile phase: CO2, i-PrOH + 0.4% i-PrNH2) to give 77 (1.0 g, 36%); LC-MS (method C): Rt = 1.77 min; mass calcd. for C19H24F2N2O2 350.2, m/z found 373.2 [M+CH3CO2]-; and 78 (1.0 g, 36%); LC-MS (method C): Rt = 1.77 min; mass calcd. for C19H24F2N2O2 350.2, m/z found 351.3 [M+H]+; [α]D20 -13.86 (c 0.38, DMF).
To a solution of 64 (80.0 g, 237 mmol) in 1,4-dioxane (1.2 L) and H2O (120 mL) were added 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (158 g, 712 mmol), CS2CO3 (216 g, 664 mmol) and Pd(dppf)Cl2.CH2Cl2 (17.8 g, 21.8 mmol). The reaction mixture was degassed 3 times and stirred at 90° C. for 20 h. The reaction mixture was concentrated under reduced pressure about ⅓ of volume. The mixture was diluted with EtOAc (500 mL) and filtered over Celite®. The filtrate was extracted with EtOAc (1 L). The organic layer was washed with water (1.5 L) and brine (300 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 20:1 to 15:1) to afford 79 (55.3 g, 64%).
To a mixture of 79 (55.3 g, 154 mmol) and A-boc-O-tosylhydroxylamine (66.2 g, 231 mmol) in t-BuOH (750 mL), CH3CN (250 mL) and H2O (250 mL) was added K2OSO4•2H2O (11.3 g, 30.7 mmol). The reaction mixture was stirred at rt for 7 h. The layers were separated, and the aqueous phase was extracted with EtOAc (1.5 L). The combined organic extracts were washed with water (1 L) and brine (300 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/CH2Cl2, gradient from 1:1 to 0:1, then petroleum ether/EtOAc 5:1) to afford 80 (49.5 g, 64%).
To a solution of 80 (29.0 g, 58.8 mmol) in CH2Cl2 (100 mL) was added HCl (4 M in EtOAc, 176.5 mL). The white suspension was stirred at rt for 20 h. The reaction mixture was diluted with petroleum ether (200 mL). The white solid was collected by filtration to give a mixture of enantiomers as a HCl salt (23 g, 91%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.25 (s, 1H), 7.62 - 7.70 (m, 2H), 7.27 - 7.35 (m, 2H), 6.25 - 7.12 (m, 1H), 5.66 (br s, 1H), 3.41 (br d, J=13.4 Hz, 1H), 3.36 - 3.49 (m, 1H), 3.09 (d, J=13.4 Hz, 1H), 1.65 (d, J=5.3 Hz, 6H), 1.14 - 1.58 (m, 1H); LC-MS (method D): Rt = 2.31 min; mass calcd. for C17H17ClF4N2O2 392.0, m/z found 393.0 [M+H]+. The enantiomers were separated by SFC (stationary phase: Daicel chiralpak IB, 20 µm, 1000 gr, mobile phase: heptane/i-PrOH (+1% i-PrNH2), 85:15) to afford 81 and 82 (25 g, 40%); [a]D22 -40.96 (c 0.415, DMF).
A mixture of 2,3-dichloropyridine-4-carbonitrile (1.33 g, 7.69 mmol), 4-fluorophenylboronic acid (1.08 g, 7.69 mmol) and K2CO3 (2 M in H2O, 7.69 mL, 15.4 mmol) in DME (25 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (0.63 g, 0.77 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 60° C. for 2 h. The reaction mixture was cooled to rt and filtered over Celite® and washed with EtOAc. The filtrate was diluted with EtOAc and brine. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 90: 10 to 70:30) to afford 83 (1.43 g, 72%, 90% pure) as a white solid.
CH3MgBr (3 M in Et2O, 5.53 mL, 16.6 mmol) was added to a solution of 83 (1.43 g, 5.53 mmol, 90% pure) in toluene (45 mL). After stirring for 5 min, Ti(Oi-Pr)4 (1.65 mL, 5.59 mmol) was added and the reaction mixture was stirred at 80° C. for 2 h and at rt for 18 h. The reaction was quenched with Na2CO3 (sat., aq.). The mixture was stirred for 30 min, filtered over Celite® and washed with EtOAc. The layers were separated, and the aqueous phase was extracted with EtOAc. The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 98:2) to afford 84 (820 mg, 56%).
To a solution of 84 (0.82 g, 310 mmol) and Et3N (0.86 mL, 6.20 mmol) in CH2Cl2 (55 mL) at 0° C. was added methanesulfonyl chloride (0.31 mL, 4.03 mmol) dropwise (the internal temperature of the reaction mixture was maintained between 0 and 3° C.). The reaction mixture was warmed to rt and stirred for 1 h. The reaction mixture was diluted with water and CH2Cl2. The layers were separated, and the organic phase was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 90: 10 to 40:60) to afford 85 (895 mg, 84%).
At 0° C., to a solution of 85 (13.00 g, 36.1 mmol, 95% pure) in CH2Cl2 (250 mL) was added m-CPBA (24.9 g, 108 mmol, 75% pure) portionwise. The reaction mixture was stirred at rt for 2 days. The reaction was quenched by the addition of NaHCO3 (sat., aq.). The layers were separated, and the aqueous phase was extracted with CH2Cl2 (4 times). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 86 (18.5 g, 86%, 60% pure).
Methanesulfonyl chloride (23.9 mL, 309 mmol) was added to a solution of 86 (18.5 g, 30.9 mmol, 60% pure) in CH2Cl2 (110 mL) at rt then heated to 70° C. for 1 day. The reaction mixture was cooled to rt and poured into NaHC03 (sat., aq.). The mixture was diluted with EtOAc. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1) to afford 87 (11.3 g, 68%, 70% pure).
In a Schlenk tube, a mixture of 87 (6.30 g, 11.7 mmol, 70% pure), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (4.85 mL, 23.4 mmol) and CS2CO3 (11.5 g, 35.2 mmol) in H2O (15 mL) and DME (95 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (1.91 g, 2.34 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 85° C. for 18 h. The reaction mixture was cooled to rt and combined with another fraction (9.28 mmol). The mixture was filtered over a pad of Celite® and washed with EtOAc and the filtrate was diluted with brine. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, 90:10) to afford 88 (14 g, quant., 65% pure).
K2OSO4.2H2O (0.88 g, 2.38 mmol) and A-boc-O-tosylhydroxylamine (5.47 g, 19.0 mmol) were added to a solution of 88 (8.00 g, 11.9 mmol, 65% pure) in t-BuOH (120 mL) and H2O (8 mL). The reaction mixture was stirred at rt for 2 days. The reaction mixture was combined with another fraction (7.44 mmol) and diluted with water, brine and EtOAc. The layers were separated and the aqueous phase was extracted with EtOAc (3 times). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 89 (21 g, 76%, 40% pure).
TFA (19.1 mL, 249 mmol) was added to a solution of 89 (22.8 g, 16.0 mmol, 40% pure) in CH2C12 (239 mL). The reaction mixture was stirred at rt for 2 days. The reaction mixture was poured into NaHCO3 (sat., aq.). The layers were separated, and the aqueous phase was extracted with CH2C12 (3 times). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CHZC12/CH30H, gradient from 100:0 to 95:5) to afford a mixture of enantiomers (2.4 g, 32%). The enantiomers were separated by SFC (stationary phase: CHIRALPAK AD-H 5 µm 250 x 30 mm, mobile phase: 72% CO2, 28% i-PrOH (0.3% i-PrNH2)) to give 90 (936 mg, 14%) and 91 (990 mg, 14%). The enantiomers were independently co-evaporated with toluene (3 times) to give 90 (920 mg, 13%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.81 (d, J=4.6 Hz, 6H), 2.89 (s, 3H), 3.09 (d, J=13.4 Hz, 1H), 3.37 (br d, J=13.6 Hz, 1H), 7.11 - 7.28 (m, 3H), 7.32 (t, J=8.9 Hz, 2H), 7.62 - 7.69 (m, 2H), 7.76 (s, 1H), 7.90 (s, 1H); LC-MS (method J): Rt = 1.29 min; mass calcd. for C18H20C1F4N3O3S 469.1, m/z found 470.5 [M+H]+; and 91 (980 mg, 14%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.81 (d, J=4.6 Hz, 6H), 2.89 (s, 3H), 3.09 (d, J=13.4 Hz, 1H), 3.38 (br d, J=13.6 Hz, 1H), 7.12 - 7.28 (m, 1H), 7.29 - 7.36 (m, 2H), 7.61 - 7.68 (m, 2H), 7.76 (s, 1H), 7.90 (s, 1H);LC-MS (method J): Rt = 1.8 min; mass calcd. for C18H20C1F4N3O3S 469.1, m/z found 470.5 [M+H]+.
The reaction was performed on 2 batches of 1.36 g of intermediate 72. In a sealed tube, a mixture of 72 (1.36 g, 4.79 mmol), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (1.50 mL, 7.19 mmol) and CS2CO3 (4.70 mg, 14.4 mmol) in H2O (2 mL) and DME (11 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (786 mg, 963 µmol) was added and the mixture was purged again with N2. The reaction mixture was heated at 120° C. in the microwave with a power output ranging from 0 to 400 W for 1 h. The two batches were combined. The reaction mixture was diluted with EtOAc and water and the layers were separated. The aqueous phase was extracted with EtOAc (twice). The combined organic extracts were washed with brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 1:0 to 7:3) to give 92 (2.15 g, 65%) as a yellow oil.
K2OSO4.2H2O (8.94 g, 24.3 mmol) and A-boc-O-tosylhydroxylamine (76.7 g, 267 mmol) were added to a solution of 92 (41.6 g, 121 mmol) in t-BuOH (1.37 L) and H2O (88 mL). The reaction mixture was stirred at rt for 16 h. The reaction mixture was diluted with water and EtOAc. The layers were separated and the aqueous phase was extracted with EtOAc (3 times). The combined organic layers were washed with NaHCO3 (sat., aq.), and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 7:3) to give 93 (41.7 g, 72%) as a pale yellow solid.
TFA (400 mL) was added to a solution of 93 (41.7 g, 87.5 mmol) in CH2C12 (650 mL) and the reaction mixture was stirred at rt for 1 h. The solvent was removed under reduced pressure. The residue was diluted with EtOAc and poured into NaHCO3 (sat., aq.). The layers were separated, and the aqueous layer was extracted with EtOAc (twice). The combined organic extracts were washed with brine, and dried (MgSOr). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/(CH3OH/aq.NH3, 95:5), gradient from 98:2 to 95:5) to afford a mixture of enantiomers (22.9 g) as a white solid. The enantiomers were separated by chiral SFC (stationary phase: CHIRALPAK IC 5 µm 250 x 30 mm, mobile phase: 84% CO2, 16% i-PrOH (0.3% i-PrNH2)) to give 94 (11.05 g) and 95 (11.09 g) both as white solids. Intermediates 94 and 95 were separately re-purified by silica column chromatography (CH2Cl2/(7 M NH3 in CH3OH, 95:5), gradient from 100:0 to 95:5) to give 94 (9.79 g, 30%); 1H NMR (400 MHz, DMSO-d6) δ ppm 8.03 (d, J=5.6 Hz, 1H), 7.94 (dd, J=7.6, 5.6 Hz, 2H), 7.36 (t, J=8.8 Hz, 2H), 6.70 (br s, 1H), 5.66 (br s, 1H), 3.47 (d, J=13.1 Hz, 1H), 3.13 (d, J=13.6 Hz, 1H), 1.55 (s, 3H) 1.54 (s, 3H), 1.25 - 1.49 (m, 2H); LC-MS (method J): Rt = 1.35 min; mass calcd. for C17H17F5N2O2 376.1, m/z found 377.3 [M+H]+; and 95 (10.3 g, 31%); 1H NMR (400 MHz, DMSO-d6) δ ppm 8.03 (d, J=5.6 Hz, 1H), 7.94 (dd, J=7.1, 6.1 Hz, 2 H), 7.36 (t, J=8.8 Hz, 2H), 6.71 (br s, 1H), 5.66 (br s, 1H), 3.47 (br d, J=13.1 Hz, 1H), 3.13 (d, J=13.6 Hz, 1H), 1.55 (s, 3H), 1.54 (s, 3H), 1.25 - 1.50 (m, 2H); LC-MS (method K): Rt = 1.27 min; mass calcd. for C17H17F5N2O2 376.1, m/z found 377.2 [M+H]+ as white solids.
To a mixture of 72 (2.00 g, 6.27 mmol, 89% pure) in CH3CN (66 mL) was slowly added BF3•OEt2 (2.45 mL, 19.4 mmol) while keeping the internal temperature at 20° C. The reaction mixture was stirred at 80° C. over the weekend. The reaction mixture was diluted with EtOAc and washed with NaHC03 (sat., aq., twice). The combined aqueous layers were extracted with EtOAc. The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 50:50) to afford 96 (949 mg, 47%) as a an off-white solid.
In a sealed tube, a mixture of 96 (949 mg, 2.92 mmol), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (1.2 mL, 5.84 mmol) and CS2CO3 (2.86 g, 8.77 mmol) in water (1.2 mL) and DME (31 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (477 mg, 584 µmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 90° C. for 20 h. Additional amount of Pd(dppf)Cl2.CH2Cl2 (239 mg, 292 µmol), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (606 µL, 2.92 mmol) and CS2CO3 (952 mg, 2.92 mmol) were added. The mixture was purged with N2 and the reaction mixture was stirred at 90° C. for another 20 h. The reaction mixture was cooled to rt and diluted with EtOAc and water. The layers were separated, and the aqueous phase was extracted with EtOAc. The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was combined with another fraction (1.59 mmol). The crude mixture was purified by silica column chromatography (heptane/(EtOAc / CH3OH, 9:1), gradient from 70:30 to 50:50) to afford 97 (1.67 g, 45%).
K2OSO4•2H2O (320 mg, 0.87 mmol) then N-boc-0-tosylhydroxylamine (1.62 g, 5.65 mmol) were added to a solution of 97 (1.67 g, 4.35 mmol) in t-BuOH (47 mL) and water (3 mL). The reaction mixture was stirred at rt for 18 h. NaHCO3 (10% aq.) and EtOAc were added. The aqueous layer was separated and extracted with EtOAc. The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 98:2) to afford 98 (1.10 g, 49%) as a pale brown solid.
TFA (1.3 mL, 17.0 mmol) was added to a solution of 98 (1.10 g, 2.13 mmol) in CH2Cl2 (21 mL) and the reaction mixture was stirred at rt overnight. NaHC03 (10%, aq.) was carefully added until pH was 7-8. The layers were separated, and the organic phase was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was combined with another fraction (77.3 µmol). The mixture was purified by silica column chromatography (CH2Cl2/(7 M NH3 in CH3OH, 9:1), gradient from 100:0 to 95:5) to afford a mixture of enantiomers 99 (675 mg, 74%) as a pale brown solid. 1H NMR (400 MHz, DMSO-d6, 23° C.) δ ppm 1.31 - 1.57 (m, 1H), 1.65 (s, 6H), 1.83 (s, 3H), 3.13 (d, J=13.4 Hz, 1H), 3.17 (s, 1H), 3.43 (br d, J=13.4 Hz, 1H), 6.10 - 7.19 (m, 1H), 7.32 - 7.41 (m, 2H), 7.65 (d, J=5.7 Hz, 1H), 7.92 (dd, J=7.5, 5.7 Hz, 2H), 8.38 (s, 1H); LC-MS (method J): Rt = 1.28 min; mass calcd. for C19H20F5N3O2 417.1, m/z found 418.4 [M+H]+.
CH3MgBr (3 M in Et2O, 64 mL, 192 mmol) was added to a solution of 2-chloro-3-fluoropyridine-4-carbonitrile (10.0 g, 63.9 mmol) in toluene (500 mL). The mixture was stirred for 5 min and Ti(Oi-Pr)4 (19.1 mL, 64.5 mmol) was added. The reaction mixture was stirred at 100° C. for 50 min. The reaction mixture was cooled to rt and the reaction was quenched with Na2CO3 (sat., aq.). The mixture was stirred overnight, filtered and the filter cake was washed with EtOAc. The layers were separated, and the aqueous phase was extracted with EtOAc. The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford crude 100 (11.9 g) that was used as such in the next step.
To a mixture of crude 100 (11.9 g) and Et3N (17.6 mL, 126 mmol) in CH2Cl2 (1 L) at 0° C. was added methanesulfonyl chloride (6.4 mL, 82.1 mmol) dropwise (the internal temperature was maintained below 0° C. during the addition). The reaction mixture was stirred for 1 h and the reaction was quenched with water (200 mL). The layers were separated and the aqueous phase was extracted. The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 96:4) to afford 101 (13.7 g, 80% over 2 steps).
A mixture of 101 (13.7 g, 51.4 mmol), 4-fluorophenylboronic acid (10.8 g, 77.1 mmol) and K2CO3 (2 M in H2O, 51.4 mL, 103 mmol) in DME (171 mL) was purged with N2. Pd(PPh3)4 (5.94 g, 5.14 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 90° C. for 4 h. The reaction mixture was cooled to rt and diluted with CH2Cl2 and water. The layers were separated and the aqueous phase was extracted with CH2Cl2. The organic phase was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified via silica column chromatography (heptane/EtOAc, gradient from 90:10 to 50:50) to afford 102 (15.8 g, 92%).
To a solution of 102 (15.8 g, 47.4 mmol) in anhydrous CH2Cl2 (591 mL) at 0° C. was added m-CPBA (16.4 g, 71.2 mmol, 75% pure). The reaction mixture was stirred at rt for 16 h. The mixture was successively washed with Na2S2O3 (sat., aq.), and NaHC03 (sat., aq., twice). The organic phase was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 103 (14.3 g, 70%, 79% pure).
To a solution of 103 (14.3 g, 33 mmol, 79% pure) in CH3CN (102 mL) was slowly added POC13 (12.3 mL, 132 mmol) and the reaction mixture was stirred at 80° C. overnight. The reaction mixture was cooled to rt and additional amount of POC13 (6.1 mL, 66 mmol) was added. The reaction mixture was stirred at 80° C. for another 2.5 h. The reaction mixture was cooled to rt and poured into ice bath (dropwise addition). The mixture was stirred for 10 min, diluted with EtOAc and basified with NaOH powder until pH was 7. The layers were separated, and the aqueous phase was extracted. The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford crude 104 (13.9 g, 75% pure).
A mixture of 104 (12.9 g, 26.8 mmol, 75% pure), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (8.35 mL, 40.3 mmol) and CS2CO3 (26.2 g, 80.5 mmol) in water (11.1 mL) and DME (61.3 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (4.38 g, 5.37 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 100° C. for 3.2 h. The reaction mixture was diluted with EtOAc and water. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were washed with brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was combined with another fraction (2.08 mmol) and purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 90:10) to afford 105 (6.90 g, 57%) as an off-white solid.
K2OSO4.2H2O (1.21 g, 3.28 mmol) and A-boc-O-tosylhydroxylamine (5.19 g, 18.1 mmol) were successively added to a solution of 105 (6.90 g, 16.4 mmol) in t-BuOH (186 mL) and water (11.9 mL). The reaction mixture was stirred at rt for 20 h. Additional amount of K2OSO4•2H2O (1.21 g, 3.28 mmol) was added and the reaction mixture was stirred at rt for another 1.5 day. Additional amount of K2OSO4.2H2O (1.21 g, 3.28 mmol) was added again and the reaction mixture was stirred at rt for another day. The reaction mixture was diluted with CH2Cl2 and NaHCO3 (sat., aq.) was added. The layers were separated, and the organic phase was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford crude 106.
TFA (74.5 mL, 974 mmol) was added to a solution of crude 106 in CH2Cl2 (121 mL). The reaction mixture was stirred at rt for 1.5 h. The reaction mixture was diluted with CH2Cl2 and poured into NaHC03 (sat., aq.). NaHC03 powder was added until pH was 7. The layers were separated and the aqueous phase was extracted with CH2Cl2. The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2C2/CH3OH, gradient from 100:0 to 96:4) to afford a mixture of enantiomers (2.89 g). The residue was purified a second time by silica column chromatography (heptane/(EtOAc/CH3OH, 9:1), gradient from 50:50 to 20:80) to afford a racemic mixture (1.90 g). The enantiomers were separated by SFC (CHIRALPAK AD-H 5 µm 250 x 30 mm, mobile phase: 90% CO2, 10% CH3OH (1.5% i-PrNH2)) to afford 107 (814 mg, 11%); 1H NMR (500 MHz, DMSO-d6) δ ppm 1.25 - 1.59 (m, 2H), 1.74 (s, 6H), 2.90 (s, 3H), 3.14 (d, J=13.6 Hz, 1H), 3.42 (d, J=13.6 Hz, 1H), 7.34 - 7.40 (m, 2H), 7.76 - 7.84 (m, 1H), 7.87 (d, J=5.4 Hz, 1H), 7.95 (dd, J=7.9, 5.7 Hz, 2H); LC-MS (method K): Rt = 1.19 min; mass calcd. for C18H20F5N3O3S 453.1, m/z found 454.3 [M+H]+; and 108 (1.0 g, 13%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.74 (br s, 6H), 2.88 (d, J=16.0 Hz, 3H), 2.99 - 3.18 (m, 2H), 3.45 - 3.76 (m, 3H), 7.31 - 7.43 (m, 2H), 7.84 - 8.00 (m, 3H); LC-MS (method K): Rt = 1.19 min; mass calcd. for C18H20F5N3O3S 453.1, m/z found 454.3 [M+H]+.
t-Butyl nitrite (3.4 mL, 29.0 mmol) was added dropwise to a mixture of 6-bromo-2-chloropyridin-3-amine (5.00 g, 24.1 mmol), (diethyloxonio)trifluoroborate (4.5 mL, 36.5 mmol) and 1,2-dimethoxyethane (30 mL) at -10° C. The reaction mixture was stirred at -10° C. for 1 h. The suspension was filtered, and the filter cake was washed with hexane (3 x 10 mL) and dried under reduced pressure to afford intermediate 6-bromo-2-chloropyridine-3-diazonium tetrafluoroborate (6.3 g), which was used in the next step without further purification. The latter was heated at 85° C. for 30 min and at 130° C. for 4 h. The mixture was purified by silica column chromatography (petroleum ether/EtOAc, 100:0 to 0:100) to afford 109 (2.53 g, 58 %) as a pink solid.
A solution of 109 (16.0 g, 76.0 mmol) in THF (50 mL) was added to a solution of LDA (2 M in THF, 57.6 mL, 115 mmol) and THF (50 mL) at -78° C. The reaction mixture was stirred at -78° C. for 1 h. Propan-2-one (24.0 mL, 322 mmol) was added dropwise at -78° C. and the reaction mixture was stirred for 3 h. The reaction was quenched with NH4Cl (sat., aq., 30 mL). The mixture was combined with another fraction (71.3 mmol) and the aqueous phase was extracted with EtOAc (3 x 50 mL). The combined organic extracts were washed with brine (20 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, 100:0 to 50:50). A second purification was performed by HPLC (PREPL-X Phenomenex luna C18 250 x 50 x 10 µm column, mobile phase: 35 to 60% (v/v) CH3CN/H2O with 0.225% FA) to afford 110 (25 g, 63%).
110 (24.0 g, 89.4 mmol), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (19.9 g, 89.6 mmol), and CS2CO3 (88.8 g, 272 mmol) were added to a solution of 1,4-dioxane (120 mL) and H2O (24 mL). The mixture was purged with Ar for 5 min and Pd(dppf)Cl2 (6.72 g, 9.18 mmol) was added. The mixture was purged with Ar for another 5 min and the reaction mixture was stirred at 70° C. for 1 h. The solid were removed by filtration and the filtrate was concentrated to dryness under reduced. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 1:0 to 4:1) to afford 111 (29.5 g, 68%, 58% pure) as a yellow oil.
K2OSO4•2H2O (4.1 g, 11.1 mmol) was added to a mixture of 111 (27.0 g, 55.5 mmol, 58% pure) and N-boc-O-tosylhydroxylamine (17.6 g, 61.3 mmol) in t-BuOH (100 mL) and H2O (15 mL). The reaction mixture was stirred at rt for 16 h. The mixture was concentrated to dryness under reduced pressure. The residue was poured into water (50 mL) and the aqueous phase was extracted with CH2Cl2 (3 x 50 mL). The combined organic extracts were washed with brine (50 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 1:0 to 5:1) to afford 112 (15 g, 44%, 68% pure) as a yellow oil.
4 M HCl (85.0 mL, 340 mmol) was added dropwise to a solution of 112 (14.0 g, 33.6 mmol) in EtOAc (50 mL). The reaction mixture was stirred at rt for 16 h. The mixture was basified with NaOH (5 M, aq.) to pH 8, and poured into water (50 mL). The layers were separated, and the aqueous phase was extracted with CH2Cl2 (3 x 100 mL). The combined organic extracts were washed with brine (20 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure to afford a mixture of enantiomers (10.17 g, 92%, 96% pure) as a white solid. The enantiomers were separated by SFC (stationary phase: Daicel Chiralpak OD H, mobile phase: heptane/i-PrOH, gradient 85:15) to afford 113.
In a vial were added 113 (0.50 g, 1.58 mmol), 3-methyl-4-fluorophenylboronic acid (486 mg, 3.16 mmol) and CS2CO3 (1.24 mg, 3.79 mmol) in 1,4-dioxane (18 mL) and water (2 mL). The vial was sealed, degassed and purged with N2 while vigorously stirred. Pd(dppf)Cl2 (57.8 mg, 78.9 µmol) was added. The vial was sealed and stirred at 90° C. for 5 h. The mixture was cooled to rt and concentrated under reduced pressure. The crude mixture was partitioned between CH2Cl2 and NaHCOs (sat., aq.). The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2CI2/7M NH3 in CH3OH, gradient from 100:0 to 90:10). Anhydrous DMF (6 mL) was added to the residue to afford a solution (118 mg/mL) of 114 (708 mg, 86%, 75% pure) in DMF. LC-MS (method G): Rt = 1.98 min; mass calcd. for C18H19F5N2O2 390.1, m/z found 391.1 [M+H]+.
A mixture of 112 (300 mg, 0.72 mmol), (3,4-difluorophenyl)boronic acid (120 mg, 0.76 mmol), K3PO4 (300 mg, 1.41 mmol) in 1,4-dioxane (5 mL) and H2O (1 mL) was purged with Ar for 5 min. Pd(dtbpf)Cl2 (20.0 mg, 30.7 µmol) was added and the mixture was purged with Ar for another 5 min. The reaction mixture was stirred at 110° C. for 12 h. The reaction mixture was cooled to rt and quenched with water (20 mL). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 20 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (petroleum ether/EtOAc, 12:1) to afford 115 (250 mg, 67%, 95% pure) as a yellow oil.
A mixture of 115 (250 mg, 506 µmol) in HCl (4 M in 1,4-dioxane, 5 mL) was stirred at rt for 12 h. The reaction mixture was concentrated to dryness under reduced pressure. The residue was dissolved in H2O (10 mL) and the solution was basified with solid NaHC03 to pH 8. The aqueous phase was extracted with EtOAc (2 x 20 mL). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated to dryness under reduced pressure. The residue was suspended in water (20 mL). The mixture was frozen and lyophilized to dryness to afford a mixture of enantiomers (170 mg, 82%), as a white solid. The enantiomers (700 mg) were separated via SFC (Chiralpak Diacel AD 20 x 250 mm, mobile phase: CO2, i-PrOH + 0.4% i-PrNH2) to afford 116 (346 mg, 49%); LC-MS (method C): Rt = 1.88 min; mass calcd. for C17H16F6N2O2 394.1, m/z found 395.1 [M+H]+; [α]D20 +47.79 (c 0.272, DMF) and 117 (354 mg, 51%); LC-MS (method C): Rt = 1.88 min; mass calcd. for C17H16F6N2O2 394.1, m/z found 395.1 [M+H]+; [α]D20 -62.65 (c 0.212, DMF).
In a Schlenk flask, a mixture of 3,5-difluoroisonicotinic acid (25.0 g, 157 mmol), iodoethane (15.0 mL, 185 mmol) and K2CO3 (23.6 g, 171 mmol) in DMF (250 mL) was stirred at 50° C. for 20 h. The reaction mixture was diluted with Et2O and water. The layers were separated, and the aqueous phase was extracted with Et2O (3 times). The combined organic layers were washed with brine (3 times), and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 118 (26 g, 57%, 64% pure) as a yellow oil.
In a Schlenk flask, to a solution of 118 (12.0 g, 41.0 mmol, 64% pure) in DCE (410 mL) was added m-CPBA (37.8 g, 164 mol, 75% pure). The reaction mixture was stirred at 80° C. for 48 h. The reaction was quenched with NaOH (1N). The layers were separated, and the organic phase was successively washed with NH4Cl (sat., aq.), Na2S2O3 (10%, aq.), water and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 119 (7.63 g, 92%) as a yellow residue.
In a sealed tube, a solution of 119 (8.41 g, 41.4 mmol) in POC13 (193 mL) was stirred at 80° C. for 20 h. The reaction mixture was concentrated under reduced pressure. The brown residue was taken up in water and EtOAc. The mixture was basified with K2CO3 powder and extracted with EtOAc (twice). The combined organic layers were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 120 (6.57 g, 72%) as a yellow oil.
In a sealed tube, a mixture of 120 (5.94 g, 28.8 mmol), 4-fluorophenylboronic acid (5.63 g, 40.2 mmol) and K2CO3 (26.8 mL) in DME (53.6 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (2.19 g, 2.68 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 80° C. for 4 h. The reaction mixture was diluted with EtOAc and water. The layers were separated and the aqueous phase was extracted with EtOAc (twice). The combined organic layers were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure, then purified by silica column chromatography (heptane/EtOAc, gradient from 49:1 to 4:1) to give 121 (5.59 g, 64%, 86% pure) as a colorless oil.
To a solution of 121 (6.87 g, 21.0 mmol, 86% pure) in DCE (212 mL) was added m-CPBA (19.3 g, 84.0 mmol, 75% pure). The reaction mixture was stirred at 80° C. for 20 h. The reaction was quenched with NaOH (1N, aq.). The organic layer was successively washed with NH4Cl (sat., aq.), Na2S2O3 (10%, aq.), water and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to give 122 (7.03 g) that was used in the next step without further purification.
In a sealed tube, a solution of 122 in POC13 (143 mL) was stirred at 80° C. for 48 h. The reaction mixture was concentrated under reduced pressure. The brown residue was reconstituted in water and EtOAc and the mixture was basified with K2CO3 powder. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic layers were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 123 (6.97 g) as a brown oil. The product was used in the next step without further purification.
CH3MgBr (3 M in Et2O, 18.4 mL, 55.2 mmol) was added dropwise to a solution of 123 in anhydrous 2-MeTHF (135 mL) at 0° C. The internal temperature was maintained near 0° C. during the addition. The reaction mixture was stirred at this temperature for 5 h. The reaction mixture was diluted with EtOAc and NH4Cl (10%, aq.) was added. The layers were separated and the aqueous phase was extracted with EtOAc (twice). The combined organic layers were washed with brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 7:3) to afford 124 (4.54 g, 68% over 3 steps) as a yellow oil that crystallized on standing.
In a sealed tube, a mixture of 124 (4.53 g, 15.0 mmol), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (4.67 mL, 22.5 mmol) and CS2CO3 (14.7 g, 45.2 mmol) in H2O (6.3 mL) and DME (34.5 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (3.68 g, 4.51 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 120° C. for 5 h. The reaction mixture was diluted with EtOAc and water. The layers were seperated and the aqueous phase was extracted with EtOAc (twice). The combined organic layers were washed with NaHCOs (sat., aq.) and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was, concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 1:0 to 7:3) to afford 125 (4.03 g, 74%).
K2OSO4.2H2O (1.82 g, 4.94 mmol) and then N-boc-O-tosylhydroxylamine (15.6 g, 54.3 mmol) were added to a solution of 125 (10.5 g, 24.7 mmol, 85% pure) in t-BuOH (280 mL) and H2O (18 mL). The reaction mixture was stirred at rt for 20 h. The reaction mixture was diluted with water and EtOAc. The layers were separated, and the aqueous phase was extracted with EtOAc (3 times). The combined organic extracts were washed with NaHC03 (sat., aq.), and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 90:10 to 80:20) to afford two fractions of 126: fraction A (10.4 g, 42%, 49% pure) and fraction B (5.8 g, 44%, 92% pure).
TFA (12 mL, 157 mmol) was added to a solution of 126 (10.4 g, 10.3 mmol, 49% pure) in CH2C12 (150 mL) and the reaction mixture was stirred at rt for 18 h. The reaction mixture was diluted with CH2C12 and poured into NaHC03 (sat., aq.). The layers were separated, and the aqueous phase was extracted with CH2C12 (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was combined with another fraction (13.8 mmol) and purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 92:8). The enantiomers were separated by SFC (stationary phase: CHIRALPAK AD-H 5 µm 250 x 30 mm, mobile phase: 85% CO2, 15% CH3OH (0.3% i-PrNH2)) to give 127 (3.64 g, 38%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.35 - 1.79 (m, 2H), 1.65 (s, 6H), 3.12 (d, J=13.4 Hz, 1H), 3.56 (br d, J= 13.4 Hz, 1H), 5.65 (s, 1H), 6.21 - 6.89 (m, 1H), 7.37 (t, J=8.9 Hz, 2H), 7.93 (br dd, J=7.2, 5.6 Hz, 2H); LC-MS (method K): Rt = 1.24 min; mass calcd. for C17H16F6N2O2 394.1, m/z found 395.2 [M+H]+; and 128 (4.35 g, 42%). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.41 - 1.75 (m, 2H), 1.65 (s, 6H), 3.12 (br d, J=13.6 Hz, 1H), 3.56 (br d, J=13.7 Hz, 1H), 5.66 (s, 1H), 6.19 - 6.90 (m, 1H), 7.37 (t, J=8.9 Hz, 2H), 7.93 (br dd, J=7.2, 5.7 Hz, 2H); LC-MS (method K): Rt = 1.24 min; mass calcd. for C17H16F6N2O2 394.1, m/z found 395.2 [M+H]+.
CH3MgI (3 M in 2-methylTHF, 220 mL, 661 mmol) was added to a solution of 3,5-difluoropyridine-4-carbonitrile (32.5 g, 220 mmol, 95% pure) in toluene (1 L). The reaction mixture was stirred for 30 min and Ti(Oi-Pr)4 (65.9 mL, 223 mmol) was added. The reaction mixture was stirred at 100° C. for 60 min. The reaction mixture was cooled to rt and the reaction was quenched with Na2CO3 (sat., aq.). The mixture was combined with another fraction (143 mmol), filtered over Celite® and washed with EtOAc. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were washed with a solution of brine and water (9:1) and concentrated to dryness under reduced pressure. The crude 129 was used as such in the next step.
To a solution of crude 129 and Et3N (66.2 mL, 476 mmol) in CH2Cl2 (820 mL) at 0° C. was added benzyl chloroformate (44 mL, 310 mmol) dropwise (the internal temperature of the reaction mixture was maintained below 0° C. during the addition). The reaction mixture was stirred for 1 h at rt. The reaction was quenched with water. The layers were separated, and the aqueous phase was extracted with CH2Cl2 (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 90:10 to 70:30) to afford 130 (7.8 g, 9% over 2 steps, 80% pure).
A mixture of 130 (2.00 g, 5.22 mmol, 80% pure) and urea hydrogen peroxide (1.03 g, 11.0 mmol) in CH3CN (30 mL) was cooled at 0° C. Trifluoroacetic anhydride (1.45 mL, 10.5 mmol) was slowly added and the reaction mixture was stirred at rt for 2 h. Na2S2O3 (10%, aq.) was added and the mixture was stirred for 15 min. The aqueous phase was extracted with CH2Cl2 (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford crude 131.
Methanesulfonyl chloride (0.67 mL, 8.69 mmol) was added to a solution of crude 131 (500 mg, 0.87 mmol, 56% pure) in DMF (5 mL) at rt. The reaction mixture was stirred at 70° C. for 2 h. The reaction mixture was poured into NaHC03 (sat., aq.) and diluted with EtOAc. The aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1) to afford 132 (270 mg, 78%).
In a Schlenk reactor, a mixture of 132 (4.60 g, 13.5 mmol), 4-fluorophenylboronic acid (2.83 g, 20.2 mmol) and K2CO3 (2 M in H2O, 13.5 mL, 27 mmol) in DME (100 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (2.21 g, 2.70 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 65° C. for 2 h. The reaction mixture was filtered over Celite® and washed with EtOAc. The filtrate was diluted with EtOAc and brine. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 90:10 to 80:20) to afford 133 (5.34 g, 79%, 80% pure).
A mixture of 133 (4.30 g, 8.59 mmol, 80% pure) and urea hydrogen peroxide (1.70 g, 18.0 mmol) in CH3CN (35 mL) was cooled at 0° C. Trifluoroacetic anhydride (2.39 mL, 17.2 mmol) was slowly added. The reaction mixture was stirred at rt for 2 h. The reaction mixture was cooled to 0° C. and additional amount of trifluoroacetic anhydride (1.19 mL, 8.59 mmol) was added. The reaction mixture was stirred at rt for another 2 h. A 10% aqueous solution of Na2S2O3 was added and the mixture was stirred for 15 min. The layers were separated, and the aqueous phase was extracted with CH2Cl2 (6 times). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford crude 134.
Methanesulfonyl chloride (6.40 mL, 82.6 mmol) was added to a solution of crude 134 (5.55 g, 8.26 mmol, 62% pure) in DMF (55 mL) at rt. The reaction mixture was stirred at 70° C. for 2 h, then poured into NaHCO3 (sat., aq.) and diluted with EtOAc. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were washed with brine (3 times), and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was combined with another fraction (1.49 mmol) and purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 90:10) to afford 135 (5.00 g, 72% pure) as a white solid.
In a Schlenk tube, a mixture of 135 (1.00 g, 1.66 mmol, 72% pure), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (0.69 mL, 3.31 mmol) and CS2CO3 (1.63 g, 4.99 mmol) in water (1.5 mL) and DME (10 mL) was purged with N2. Pd(dppf)Cl2.CH2Cl2 (135 mg, 0.17 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 80° C. for 2 days. The reaction mixture was diluted with EtOAc and water and filtered over Celite®. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was partially purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 85:15) to afford two fractions of 136: fraction A (440 mg, 28%, 53% pure) and fraction B (630 mg, 55%, 71% pure).
K2OSO4.2H2O (66.7 mg, 0.18 mmol) and N-boc-O-tosylhydroxylamine (572 mg, 1.99 mmol) were successively added to a solution of 136 (630 mg, 905 µmol, 71% pure) in t-BuOH (10 mL) and water (0.66 mL). The reaction mixture was stirred at rt for 20 h. The reaction mixture was diluted with water and EtOAc. The layers were separated, and the aqueous phase was extracted with EtOAc (3 times). The combined organic extracts were washed with NaHCOs (sat., aq.), and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 90:10 to 80:20) to afford two fractions of 137: fraction A (420 mg, 35%, 47% pure) and fraction B (50 mg, 7%, 80% pure).
A solution of 137 (420 mg, 315 µmol; 47% pure) in EtOH (10 mL) was stirred under N2 atmosphere. 10% Pd/C (66.9 mg) was added and the reaction mixture was stirred at rt under 5 bar H2 for 3 h. The reaction mixture was filtered over Celite® and washed with EtOH. The filtrate was concentrated to dryness under reduced pressure. The crude mixture was purified by silica column chromatography (CH2C12/CH3OH, gradient from 100:0 to 98:2) to afford 138 (103 mg, 66%).
At 0° C., acetyl chloride (238 µL, 3.34 mmol) was added to a mixture of 138 (1.10 g, 2.23 mmol) and DMAP (545 mg, 4.46 mmol) in CH2Cl2 (15 mL). The reaction mixture was stirred at rt for 18 h. The reaction mixture was diluted with water and CH2Cl2. The layers were separated and the organic phase was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 90:10 to 60:40) to afford 139 (1.17 g, 93%).
TFA (794 µL, 10.4 mmol) was added to a solution of 139 (1.17 g, 2.08 mmol) in CH2Cl2 (30 mL) and the reaction mixture was stirred at rt for 18 h. Additional quantity of TFA (794 µL, 10.4 mmol) was added and the reaction mixture was stirred at rt for another 3 h. The reaction mixture was poured into NaHCOs (sat., aq.). The layers were separated, and the aqueous phase was extracted with CH2Cl2 (3 times). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The enantiomers were separated by SFC (CHIRALPAK AD-H 5 µm 250 x 30 mm, mobile phase: 85% CO2, 15% i-PrOH (0.3% i-PrNH2)) to give 140 (378 mg, 41%); 1H NM+R (400 MHz, DMSO-d6) δ ppm 1.65 (br dd, J=8.8, 2.6 Hz, 1H), 1.71 (br s, 6H), 1.78 (s, 3H), 3.11 (d, J=13.7 Hz, 1H), 3.52 (br d, J=13.8 Hz, 1H), 6.01 - 7.02 (m, 1H), 7.33 - 7.43 (m, 2H), 7.91 (br dd, J=7.2, 5.6 Hz, 2H), 8.57 (s, 1H); and 141 that was re-purified via chiral SFC (stationary phase: CHIRALPAK AD-H 5 µm 250 x 30 mm, mobile phase: 87% CO2, 13% i-PrOH (0.3% i-PrNH2)) to give 141 (359 mg, 40%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.59 - 1.67 (m, 1H), 1.71 (br s, 6H), 1.78 (s, 3H), 3.11 (d, J=13.6 Hz, 1H), 3.52 (br d, J=13.6 Hz, 1H), 6.20 - 6.91 (m, 1H), 7.33 - 7.41 (m, 2H), 7.88 - 7.95 (m, 2H), 8.57 (s, 1H).
To a solution of 138 (1.10 g, 2.19 mmol) in CH2Cl2 (22 mL) at 0° C. was added methanesulfonyl chloride (187 µl, 2.40 mmol) dropwise (the internal temperature of the reaction mixture was maintained between 0 and 3° C. during the addition). Et3N (304 µL, 2.19 mmol) was added and the reaction mixture was stirred at rt for 18 h. Additional amount of methanesulfonyl chloride (102 µL, 1.31 mmol) and Et3N (182 µL, 1.31 mmol) were added and the reaction mixture was stirred at rt for another 2 h. Methanesulfonyl chloride (84.8 µL, 1.09 mmol) and Et3N (182 [tL, 1.31 mmol) were added. The reaction mixture was stirred for 2 h and extra amounts of methanesulfonyl chloride (50.9 [tL, 0.66 mmol) and Et3N (152 [tL, 1.09 mmol) were added. The reaction mixture was stirred at for another 2 h and the reaction was quenched with NaHCOs (sat., aq.). The mixture was diluted with CH2Cl2. The layers were separated, and the aqueous phase was extracted with CH2Cl2 (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 90:10 to 60:40) to afford 142 (1.26 g, 90%, 89% pure).
144 TFA (2.23 mL, 29.2 mmol) was added to a solution of 142 (1.25 g, 1.95 mmol) in CH2Cl2 (34 mL) and the reaction mixture was stirred at rt for 18 h. The reaction mixture was poured into NaHCOs (sat., aq.). The layers were separated and the aqueous phase was extracted with CH2Cl2 (3 times). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The enantiomers were separated by SFC (stationary phase: Chiralpak IG 5 µm 250 x 20 mm, mobile phase: 90% CO2, 10% CH3OH (0.3% i-PrNH2)) to give 143 (394 mg, 43%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.81 (br d, J=7.7 Hz, 6H), 2.79 - 2.86 (m, 1H), 2.87 (s, 3H), 3.12 (br d, J=13.6 Hz, 1H), 3.54 (br d, J=13.9 Hz, 1H), 6.20 - 6.99 (m, 1H), 7.35 -7.43 (m, 2H), 7.82-8.14 (m, 3H); and 144 (397 mg, 43%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.81 (br d, J=7.7 Hz, 6H), 2.79 - 2.86 (m, 1H), 2.87 (s, 3H), 3.12 (br d, J=13.7 Hz, 1H), 3.54 (br d, J=13.7 Hz, 1H), 6.27 - 6.86 (m, 1H), 7.33 - 7.44 (m, 2H), 7.81 - 8.15 (m, 3H).
To a solution of 123 (5.05 g, 16.0 mmol) in 2-methylTHF (30 mL) at 0° C. was added CH3MgBr (1 M in THF, 160 mL, 160 mmol). The reaction mixture was stirred at 70° C. for 12 h, cooled to rt and quenched with NH4Cl (aq.). The layers were separated, and the aqueous phase was extracted with EtOAc (3 x 150 mL). The combined organic extracts were washed with brine, and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 85:15) to afford 145 (2.2 g, 47%) as a white solid.
In a Schlenk tube, a mixture of 145 (2.00 g, 6.72 mmol), 2-(1-cyclopropylvinyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (1.56 g, 8.06 mmol) and Cs2CO3 (6.57 g, 20.2 mmol) in H2O (7.11 mL) and DME (35.5 mL) was purged with N2. PdCl2(dppf) (246 mg, 0.34 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 80° C. for 16 h. The mixture was diluted with EtOAc and water, and the layers were separated. The aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 75:25) to afford 146 (1.0 g, 45%).
To a solution of 146 (1.00 g, 3.04 mmol) and N-boc-O-tosylhydroxylamine (1.31 g, 4.55 mmol) in t-BuOH (30 mL), CH3CN (10 mL) and H2O (5.34 mL) was added K2OSO4•2H2O (224 mg, 0.61 mmol). The reaction mixture was stirred at rt for 18 h. The solvent was removed under reduced pressure and the residual fraction was dissolved in EtOAc and washed with NaHCO3 (sat., aq.) and brine. The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 50:50) to afford 147 (700 mg, 50%).
A solution of 147 (400 mg, 0.87 mmol) in CH2Cl2 (10 mL) was treated with TFA (2 mL, 26. mmol) at rt. The reaction mixture was stirred for 4 h and the solvent was removed under reduced pressure. The residue was dissolved in EtOAc and washed with NaHCO3 (sat., aq.) and water. The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 148 (310 mg, 99%) which was used as such in the next step. LC-MS (method B): Rt = 0.90 min; mass calcd. for C20H24F2N2O2 362.2, m/z found 363.3 [M+H]+.
A mixture of 2,3-dichloro-5-fluoropyridine (20.0 g, 120 mmol), 4-fluorophenylboronic acid (17.7 g, 127 mmol) and K2CO3 (2 M in H2O, 120 mL) in DME (400 mL) was purged with N2. Pd(dppf)Cl2. CH2Cl2 (6.89 g, 8.44 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 80° C. for 2 h. The reaction mixture was cooled to rt and filtered through packed Celite®. The filter cake was washed with EtOAc. The filtrate was diluted with EtOAc and brine. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 80:20) to afford 2 fractions of 149 a pure fraction (16.6 g, 61%) and a fraction containing impurities (9.00 g, 20%, 60% pure).
A solution of 149 (108 g, 479 mmol) and n-BuLi (1.6 M in hexane, 330 mL, 528 mmol) in THF (1 L) was stirred at -75° C. for 2 h. CO2 was bubbled in the reaction mixture for 1 h. Volatiles were evaporated, and the residue was taken up in HCl (3 M aq.). The solids were collected by filtration, washed with water and dried at 70° C. for 50 min to afford 150 (113 g, 88%).
A mixture of 150 (117 g, 434 mmol), CH3I (32.4 mL, 521 mmol) and K2CO3 (132 g, 955 mmol) in DMF (900 mL) was stirred at rt for 18 h. The reaction mixture was diluted with EtOAc and a solution of brine and water (4:1). The layers were separated, and the aqueous phase was extracted with EtOAc (3 times). The combined organic extracts were washed with a solution of brine and water (9:1) (4 times). The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 151 (148 g, quant., 83% pure) as a yellow oil which crystalized on standing.
At 0° C., to a solution of 151 (117 g, 343 mmol, 83% pure) in CH2Cl2 (1.3 L) was added m-CPBA (158 g, 686 mmol, 75% pure) portion wise. The reaction mixture was stirred at rt for 24 h. Additional amount of m-CPBA (158 g, 686 mmol, 75% pure) was added portionwise and the reaction mixture was stirred at rt for 6 h, and at 45° C. for 48 h. The reaction was quenched with Na2S2CO3 (sat., aq.). The layers were separated, and the aqueous phase was extracted with CH2Cl2 (3 times). The combined organic extracts were washed with a NaOH (1 M, aq.), and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 152 (119 g, quant., 86% pure) as a yellow solid.
To a solution of 152 (88.0 g, 253 mmol, 86% pure) in CH3CN (880 mL) was added POCl3 (93.9 mL, 1.01 mol) and the reaction mixture was stirred at 80° C. for 18 h. The reaction mixture was poured dropwise into a solution of water, EtOAc and NaHCO3 (sat., aq.). The mixture was combined to another fraction (115 mmol). The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was combined with another mixture (43.0 mmol). The residue was diluted with Et2O and the suspension was stirred at rt for 5 min. The solids were collected by filtration, washed with Et2O and dried to afford a first crop of 153 (83 g, 64%) as an off-white solid. The filtrate was evaporated, and the residue was dissolved in Et2O. The suspension was stirred at rt for 5 min, and the solids were collected by filtration, washed with Et2O and dried to afford a second crop of 153 (28 g, 22%) as an off-white powder. The filtrate was concentrated under reduced pressure and purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 90:10) to afford a third crop of 153 (25 g, 11%, 60% pure).
Under N2 atmosphere, CH3MgBr (3 M in Et2O, 50.3 mL, 151 mmol) was added dropwise to a solution of 153 (16.0 g, 50.3 mmol) in anhydrous 2-MeTHF (250 mL) in an ice bath (the internal temperature was maintained around -2° C.). The reaction mixture was stirred at this temperature for 30 min and at rt for 18 h. The reaction mixture was diluted with EtOAc and NH4Cl (10%, aq.) was added. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were washed with brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 85:15) to afford 154 (4.11 g, 26%) as a colorless oil.
A mixture of 154 (15.8 g, 49.7 mmol), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (20.6 mL, 99.3 mmol) and CS2CO3 (48.7 g, 150 mmol) in H2O (35.6 mL) and DME (198 mL) was purged with N2. Pd(dppf)Cl2. CH2Cl2 (4.06 g, 4.97 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 80° C. for 7 h and at rt for 15 h. The reaction mixture was diluted with EtOAc and water. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 80:20) to afford 155 (16.7 g, 45%, 50% pure) as an oil.
K2OSO4•2H2O (2.08 g, 5.64 mmol) and N-boc-O-tosylhydroxylamine (17.8 g, 62.0 mmol) were added to a solution of 155 (14.2 g, 28.2 mmol, 75% pure) in t-BuOH (320 mL) and H2O (20.5 mL). The reaction mixture was stirred at rt for 20 h. The reaction mixture was diluted with water and EtOAc. The mixture was combined with another fraction (19.8 mmol). The layers were separated, and the aqueous phase was extracted with EtOAc (3 times). The combined organic extracts were washed with NaHCO3 (sat., aq.), and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude product 156 was used in the next step without further purification.
TFA (57 mL, 745 mmol) was added to a solution of 156 in CH2Cl2 (710 mL) and the reaction mixture was stirred at rt for 18 h. The reaction mixture was poured slowly into NaHCO3 (sat., aq.). The layers were separated, and the aqueous phase was extracted with CH2Cl2 (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 92:8) to afford a mixture of enantiomers (15.8 g, 79% over 2 steps). The enantiomers were separated via chiral SFC (stationary phase: CHIRALPAK AD-H 5 µm 250 x 30 mm, mobile phase: 85% CO2, 15% CH3OH (0.6% Et3N)) to afford 157 (7.8 g, 40%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.58 -1.81 (m, 1H), 1.69 (br s, 6H), 3.08 (d, J=13.6 Hz, 1H), 3.51 (br d, J= 13.7 Hz, 1H), 5.56 (s, 1H), 5.98 - 7.03 (m, 1H), 7.28 - 7.36 (m, 2H), 7.59 - 7.68 (m, 2H);_LC-MS (method K): Rt = 1.26 min; mass calcd. for C17H16ClF5N2O2 410.1, m/z found 411.2 [M+H]+; and 158 (7.38 g, 37%); 1H NMR (400 MHz, DMSO-d6) δ ppm 1.54 - 1.73 (m, 1H), 1.69 (br s, 6H), 3.08 (br d, J=13.6 Hz, 1H), 3.51 (br d, J=13.4 Hz, 1H), 5.55 (s, 1H), 5.91 - 7.00 (m, 1H), 7.32 (t, J=8.9 Hz, 2H), 7.64 (dd, J=8.6, 5.6 Hz, 2H);_LC-MS (method K): Rt = 1.26 min; mass calcd. for C17H16ClF5N2O2 410.1, m/z found 411.2 [M+H]+.
To a solution of 69 (10.0 g, 40.1 mmol) in anhydrous 2-MeTHF (240 mL) at 0° C. under N2 atmosphere was added CH3MgBr (3 M, 33.4 mL, 100 mmol). The reaction mixture was stirred at rt for 18 h. the mixture was diluted with EtOAc and quenched with NH4Cl (10%, aq.). The layers were separated, and the organic phase was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 70:30) to afford 159 (7.66 g, 77%) as an off-white solid.
t-Butyldimethylsilyl trifluoromethanesulfonate (14.0 mL, 61.0 mmol) was added dropwise to a solution of 159 (7.60 g, 30.5 mmol) and 2,6-dimethylpyridine (10.7 mL, 91.5 mmol) in CH2Cl2 (160 mL). The reaction mixture was stirred at rt for 4h. The reaction was diluted with water. The layers were separated, and the organic phase was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 70:30) to afford 160 (10.3 g, 93%) as a white solid.
To a solution of 160 (2.00 g, 5.50 mmol) in 2-MeTHF was added BF3.OEt2 (9.90 mmol) at -10° C. The mixture was stirred for 15 min and 2,2,6,6-tetramethylpiperidinylmagnesium chloride LiCl complex (solution in 2-MeTHF, 16.5 mmol). The mixture was stirred at this temperature for 3.5 h and a solution of I2 (16.5 mmol) in 2-MeTHF was added while maintaining the temperature of the mixture below 20° C. The reaction mixture was stirred at this temperature for 1.5 h. The reaction was quenched with Na2CO3 (aq.) and the mixture warmed to rt. Na2S2O3 (aq.) was added and the mixture was stirred for 30 min and filtered over Celite®. The layers were separated, and the organic phase was concentrated under reduced pressure. The residue was taken up in CH3OH and concentrated several times under reduced pressure to afford 161.
A mixture of 161 (5.00 g, 10.2 mmol) and 2,2-difluoro-N-methoxy-N-methylacetamide (1.43 mL, 12.3 mmol) in THF (83 mL) was stirred at -50° C. i-PrMgCl•LiCl (1.3 M in THF, 19.6 mL, 25.5 mmol) was added dropwise. The reaction mixture was stirred at -50° C. for 1 h and warmed to 0° C. The reaction was quenched with NH4Cl (sat., aq., 10 mL). The layers were separated, and the aqueous phase was extracted with EtOAc. The combined organic extracts were concentrated under reduced pressure. The crude mixture was combined with another fraction (2.04 mmol). The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 98:2 to 95:5) to afford 162 (2.45 g, 85% pure).
To a solution of methyltriphenylphosphonium chloride (1.91 g, 6.12 mmol) in THF (100 mL) was added t-BuOK (686 mg, 6.12 mmol). The mixture was stirred at 15° C. for 30 min. A solution of 162 (2.25 g, 5.10 mmol) in THF (25 mL) was added dropwise, and the reaction mixture was stirred at 15° C. for 16 h. Additional amount of t-BuOK (686 mg, 6.12 mmol) and methyltriphenylphosphonium chloride (1.91 g, 6.12 mmol) were added at 15° C. and the reaction mixture was stirred for another 18 h. The reaction mixture was diluted with water (100 mL) and extracted with EtOAc (2 x 300 mL). The combined organic extracts were washed with brine (100 mL) and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (CH2Cl2) to afford 163 (1.2 g, 54%) as a white solid.
A mixture of 163 (1.10 g, 2.50 mmol) and TBAF (1 M in THF, 5.00 mL, 5.00 mmol) in THF (20 mL) was stirred at rt for 2 h. The reaction mixture was diluted with water and EtOAc. The layers were separated, and the organic layer was washed with brine and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2) to afford 164 (800 mg, 98%).
To a solution of 164 (800 mg, 2.46 mmol) and t-butyl ([(4-methylphenyl)sulfonyl]oxy)carbamate (1.07 g, 3.74 mmol) in t-BuOH (30 mL), CH3CN (10 mL) and H2O (4.3 mL) was added K2OSO4•2H2O (181 mg, 0.49 mmol). The reaction mixture was stirred at rt for 18 h. The reaction mixture was extracted with EtOAc, washed with water (twice) and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 90:10) to afford 165 (950 mg, 84%).
A solution of 165 (940 mg, 2.05 mmol) in CH2Cl2 (25 mL) was treated with TFA (5 mL) at rt. The reaction mixture was stirred for 3 h. The solvent was removed under reduced pressure and the residue was dissolved in EtOAc and washed with water. The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was evaporated under reduced pressure to dryness. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 95:5 to 90:10) to afford 166 (412 mg, 56%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm 7.92 - 7.99 (m, 3H), 7.31 - 7.38 (m, 2H), 6.34 (t, J=54.8 Hz, 1H), 5.89 - 6.12 (m, 1H), 5.60 (s, 1H), 3.08 - 3.14 (m, 1H), 2.99 - 3.05 (m, 1H), 1.55 (s, 6H), 1.32 - 1.61 (m, 2H); LC-MS (method B): Rt = 0.86 min; mass calcd. for C17H18F4N2O2 358.1, m/z found 359.0 [M+H]+.
To a solution 64 (5.33 g, 17.7 mmol) in 1,4-dioxane (76 mL) were added a solution of Cs2CO3 (16.2 g, 49.7 mmol) in water (8 mL), 2-(1-cyclopropylvinyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (6.91 g, 35.6 mmol) and Pd(dppf)Cl2. CH2Cl2 (1.52 g, 1.86 mmol) under N2. The reaction mixture was stirred at 90° C. for 20 h in a sealed reactor. The reaction mixture was cooled to rt, diluted with EtOAc and filtered over Celite®. The filtrate was extracted with EtOAc. The combined organic extracts were washed with water and brine, and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2) to afford 167 (3.74 g, 64%) as a yellow solid.
To a solution of 167 (3.67 g, 11.1 mmol) and N-boc-O-tosylhydroxylamine (9.20 g, 32.0 mmol) in t-BuOH (65 mL), CH3CN (18 mL) and H2O (18 mL) was added K2OSO4•2H2O (815 mg, 2.21 mmol). The dark mixture was stirred at rt for 7 h. The mixture was extracted with EtOAc. The combined organic extracts were washed with water and brine, dried (MgSO4), filtered and concentrated under reduced pressure. The crude mixture was purified by flash column chromatography (CH2Cl2/(CH2Cl2/CH3OH, gradient from 100:0 to 90:10) to afford 168 (3.72 g, 72%) as an oil.
To a solution of 168 (3.70 g, 7.96 mmol) in CH2Cl2 (50 mL) was added TFA (6.09 mL, 79.6 mmol). The reaction mixture was stirred at rt for 2 h. The reaction mixture was concentrated under reduced pressure. The residue was diluted with CH2Cl2 and the solution was washed with NaHCO3 (sat., aq., twice) and water. The desired product precipitated and was collected by filtration. 1H NMR (400 MHz, DMSO-d6, 27° C.) δ ppm 0.06-0.15 (m, 1H), 0.24-0.38 (m, 2H), 0.42-0.50 (m, 1H), 1.18-1.50 (m, 3H), 1.64 (d, J=5.3 Hz, 6H), 2.81 (br d, J=13.2 Hz, 1H), 3.14 (br d, J=13.0 Hz, 1H), 4.83 (br s, 1 H), 5.54 (s, 1H), 7.28 (t, J=8.9 Hz, 2H), 7.61-7.69 (m, 2H), 8.06 (s, 1H). The enantiomers were separated via SFC (stationary phase: Daicel Chiralpak AD_H 5 µm 300 g, mobile phase: heptane (0.2% i-PrNH2)/EtOH (0.2% i-PrNH2), gradient from 100:0 to 95:5) to afford 169 (800 mg); [α]D20 -38.93 (c 0.614, DMF); and 170 (800 mg); [α]D20 +38.0 (c 0.25, DMF).
Into a microwave vial equipped with a magnetic stir bar were added tributyl(1-ethoxyvinyl)tin (6.75 mL, 19.4 mmol) and 72 (5.00 g, 17.6 mmol) in 1,4-dioxane (15 mL). Pd(PPh3)2Cl2 (1.24 g, 1.76 mmol) was added and the reaction mixture was stirred at 100° C. for 6 h. The reaction mixture was cooled to rt and filtered through Celite®. The filter cake was washed with 1,4-dioxane (3 times) and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 0:100) to afford 171 (1.68 g, 30%).
To a solution of 171 (1.68 g, 5.26 mmol) in THF (14 mL) and water (4 mL) was added NBS (0.94 g, 5.26 mmol) at 0° C. The reaction mixture warmed to rt and was stirred for 5 h. The reaction mixture was diluted with water and the mixture was extracted with EtOAc (3 x 200 mL). The combined organic layers were washed with NaHCO3 (sat., aq.) and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 172 (crude) as a yellow solid. The crude was used in the next step without purification.
Crude 172 (11 g) was added to a suspension of K2CO3 (4.50 g, 32.7 mmol) and dibenzylamine (6 mL, 31.2 mmol) in DMF (40 mL). The reaction mixture was stirred at rt for 2 h. The reaction mixture was diluted with water (600 mL) and partitioned with EtOAc (4 x 200 mL). The organic layers were combined and dried (MgSO4). The solids were removed by filtration and the filtrate was passed through packed alumina and concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 0:100) to afford 173 (1.38 g) as a yellow oil.
Into a microwave vial equipped with a magnetic stir bar were added 173 (6.68 g, 13.7 mmol, not pure) in anhydrous THF (100 mL). The vial was sealed and cooled to 0° C. Then, cyclopropylmagnesium bromide (1 M in 2-MeTHF, 70 mL, 70 mmol) was added. The reaction was allowed to reach rt over 3 h. An additional amount of cyclopropylmagnesium bromide (1 M in 2-MeTHF, 27 mL, 27 mmol) was added at rt. The reaction mixture was stirred for 3 h. The reaction mixture was diluted with CH3OH (5 mL), and NaHCO3 (sat., aq.) was added. The layers were separated, and the aqueous phase was extracted with EtOAc (5 x 200 mL). The combined organic layers were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (heptane/EtOAc, gradient from 100:0 to 80:20) to afford 174 (2.83 g, 39%) as a yellow oil.
Into an erlenmeyer flask were added a solution of 174 (2.83 g, not pure) in CH3OH (100 mL). The flask was sparged with N2 and 10%Pd/C (700 mg) was added. The flask was sealed and exposed to H2. After 4 h at rt, the reaction mixture was filtered through Celite®. The filtrate was concentrated under reduced pressure. The enantiomers were separated by SFC (stationary phase: Chiralpak Daicel IC 20 x 250 mm, mobile phase: CO2, EtOH + 0.4% i-PrNH2) to afford 175 (507 mg, 27%); LC-MS (method C): Rt = 1.55 min; mass calcd. for C19H22F2N2O2 348.2, m/z found 349 [M+H]+; [α]D20 -34.7 (c 0.36, DMF); and 176 (503 mg, 27%); LC-MS (method C): Rt = 1.56 min; mass calcd. for C19H22F2N2O2 348.2, m/z found 349 [M+H]+; [α]D20 +199.2 (c 0.125, DMF).
To a solution of 110 (50.0 g, 168 mmol) in Et2O (900 mL) was added n-BuLi (2.5 M in hexanes, 134 mL, 335 mmol) at -78° C. The reaction mixture was stirred at -78° C. for 10 min and a solution of cyclopropanecarboxylic acid N-methoxy-N-methylamide (32.5 g, 251 mmol) in Et2O (100 mL) was added. The reaction mixture was stirred for 10 min at -78° C. and at 15° C. for 1 h. NH4Cl (sat., aq., 100 mL) was added. The layers were separated, and the aqueous phase was extracted with EtOAc (2 x 200 mL). The combined extracts were washed with water (100 mL) and brine (100 mL) and concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 177 (170 g, 98%) as a yellow oil.
To a solution of methyl(triphenyl)phosphonium bromide (156 g, 437 mmol) in THF (500 mL) was added t-BuOK (49.0 g, 437 mmol) and the mixture was stirred at 15° C. for 30 min. To the mixture was added dropwise a solution of 177 (93.8 g, 364.1 mmol) in THF (200 mL) and the reaction mixture was stirred at 15° C. for 12 h. The reaction mixture was diluted with water (200 mL) and the aqueous phase was extracted with EtOAc (2 x 300 mL). The combined organic extracts were washed with water (200 mL) and brine (200 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 90:10) to afford 178 (70 g, 75 %) as a yellow solid.
To a solution of 178 (88.0 g, 310 mmol) in t-BuOH (800 mL) and H2O (160 mL) was added N-Boc-O-tosylhydroxylamine (107 g, 372 mmol). K2OSO4•2H2O (17.2 g, 46.5 mmol) was added and the reaction mixture was stirred at 40° C. for 16 h. The reaction mixture was diluted with water (200 mL) and the layers were separated. The aqueous phase was extracted with EtOAc (2 x 300 mL). The combined organic extracts were washed with water (200 mL) and brine (200 mL), and dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica column chromatography (petroleum ether/EtOAc, gradient from 100:0 to 50:50) to afford 179 (60 g, 50%) as a yellow oil.
To a solution of 179 (60.0 g, 154 mmol) in EtOAc (200 mL) was added HCl (4 M in EtOAc, 300 mL). The white suspension was stirred at rt for 16 h. The reaction mixture was poured into petroleum ether (1.5 L) and the solid was collected by filtration. The solid was basified with NaOH (0.5 M, aq., 1.2 L) and extracted with a mixture of CH2Cl2 and CH3OH (10:1, 2 x 1.3 L). The combined organic extracts were dried (Na2SO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was triturated in petroleum ether and CH2Cl2 (10: 1, 400 mL), and collected by filtration to afford a mixture of enantiomers (21 g, 45%, 95% purity) as white solid. The enantiomers were separated via SFC (stationary phase: Chiralpak Diacel AD 20 x 250 mm, mobile phase: CO2, i-PrOH + 0.4% i-PrNH2) to afford 180; [α]D20 -32.6 (c 0.31, DMF); and 181; [α]D20 +28.9 (c 0.35, DMF).
To a mixture of 181 (2.89 g, 10.0 mmol) and 4-(trifluoromethyl)phenylboronic acid (2.28 g, 12.0 mmol) in 1,4-dioxane (90 mL) was added a solution of CS2CO3 (7.20 g, 22.0 mmol) in water (10 mL). The mixture was degassed for 15 min with N2. PdCl2(dppf) (368 mg, 0.50 mmol) was added and the reaction mixture was stirred at 50° C. for 1 h. The reaction mixture was cooled to rt and stirred overnight. The residue was dissolved in CH2Cl2. The solution was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/7M NH3 in CH3OH, 95/5). The residue was triturated in DIPE and the precipitate was collected by filtration and dried under vacuum to afford 182 (1.93 g, 48%). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.08 - 0.16 (m, 1H), 0.25 - 0.40 (m, 2H), 0.41 - 0.51 (m, 1H), 1.28 - 1.36 (m, 1H), 1.56 (d, J=4.9 Hz, 6H), 2.87 (d, J=13.0 Hz, 1H), 3.20 (d, J=13.0 Hz, 1H), 4.87 (s, 1H), 5.59 (s, 1H), 7.88 (d, J=8.1 Hz, 2H), 7.93 (d, J=5.7 Hz, 1H), 8.11 (d, J=8.1 Hz, 2H); LC-MS (method H): Rt = 1.90 min; mass calcd. for C20H22F4N2O2 398.2, m/z found 399.3 [M+H]+.
To a mixture of 181 (2.89 g, 10 mmol) and 4-chlorophenylboronic acid (2.00 g, 12.79 mmol) in 1,4-dioxane (90 mL) was added a solution of CS2CO3 (7.20 g, 22.0 mmol) in water (10 mL). The mixture was degassed for 15 min with N2. PdCl2(dppf) (368 mg, 0.50 mmol) was added and the reaction mixture was stirred at 70° C. for 4 h. The mixture was cooled to rt and stirred overnight under N2. The residue was dissolved in CH2Cl2 and dried (MgSO4). The solids were removed by filtration and the filtrate was evaporated under reduced pressure. The crude was purified by silica column chromatography (CH2Cl2/7M NH3 in CH3OH, 95/5). The crude was triturated in DIPE. The precipitate was collected by filtration and dried under vacuum to afford 183 (2.2 g, 60%). LC-MS (method H): Rt = 1.76 min; mass calcd. for C19H22ClFN2O2 364.1, m/z found 365.1 [M+H]+.
A microwave vial was charged with 181 (0.25 g, 0.87 mmol), 4-cyclopropylphenylboronic acid (168 mg, 1.04 mmol), CS2CO3 (846 mg, 2.60 mmol), 1,4-dioxane (5 mL), and water (1 mL). The vial was sealed and degassed with N2. Pd(dppf)Cl2 (31.7 mg, 43.3 µmol) was added and the vial was sealed. The reaction mixture was shacked at 90° C. for 5 h. The mixture was diluted with CH2Cl2 and partitioned with water. The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/7M NH3 in CH3OH, gradient from 100:0 to 90:10) to afford 184 (195 mg, 43%, 71% pure). LC-MS (method G): Rt = 1.96 min; mass calcd. for C22H27FN2O2 370.2, m/z found 371.2 [M+H]+.
A microwave vial was charged with 181 (0.25 g, 0.86 mmol), 3-fluorophenylboronic acid (144 mg, 1.03 mmol), CS2CO3 (840 mg, 2.58 mmol), 1,4-dioxane (5 mL), and water (1 mL). The vial was sealed and degassed with N2. Pd(dppf)Cl2 (31.4 mg, 43.0 µmol) was added and the vial was sealed. The reaction mixture was shacked at 90° C. for 5 h. The mixture was diluted with CH2Cl2 and partitioned with water. The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/7M NH3 in CH3OH, gradient from 100:0 to 90:10) to afford 185 (190 mg, 53%, 83% pure). LC-MS (method G): Rt = 1.71 min; mass calcd. for C19H22F2N2O2 348.2, m/z found 349.2 [M+H]+.
To a mixture of 181 (3.46 g, 12.0 mmol) and 3,4-difluorophenylboronic acid (2.27 g, 14.4 mmol) in 1,4-dioxane (90 mL) was added a solution of CS2CO3 (8.60 g, 26.4 mmol) in water (10 mL). The mixture was degassed for 15 min with N2. PdCl2(dppf) (460 mg, 0.62 mmol) was added and the reaction mixture was stirred at 90° C. for 1 h. The reaction mixture was cooled to rt and stirred overnight. The residue was dissolved in CH2Cl2. The solution was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude mixture was purified by silica column chromatography (CH2Cl2/7M NH3 in CH3OH, 95/5). The residue was triturated in DIPE, and the precipitate was collected by filtration and dried under vacuum to afford 186 (2.55 g, 58%). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.07 - 0.15 (m, 1H), 0.24 - 0.39 (m, 2H), 0.43 - 0.51 (m, 1H), 1.27 - 1.36 (m, 1H), 1.54 (d, J=5.3 Hz, 6H), 2.86 (d, J=13.0 Hz, 1H), 3.19 (d, J=13.0 Hz, 1H), 4.85 (s, 1H), 5.57 (s, 1H), 7.57 (dt, J=10.6, 8.5 Hz, 1H), 7.76 - 7.82 (m, 1H), 7.88 (d, J=5.7 Hz, 1H), 7.94 (ddd, J=11.7, 8.2, 1.6 Hz, 1H); LC-MS (method H): Rt = 1.76 min; mass calcd. for C19H21F3N2O2 366.2, m/z found 367.3 [M+H]+.
In a Schlenk tube, a mixture of 124 (1.00 g, 3.15 mmol), 2-(1-cyclopropylvinyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (611 mg, 3.15 mmol) and CS2CO3 (3.09 g, 9.49 mmol) in water (2.6 mL) and DME (16.6 mL) was purged with N2. Pd(dppf)Cl2. CH2Cl2 complex (129 mg, 0.16 mmol) was added and the mixture was purged again with N2. The reaction mixture was stirred at 80° C. for 7 h then at rt for 15 h. The reaction mixture was diluted with EtOAc and water. The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were dried (MgSO4). The solids were removed by filtration and the filtrate was evaporated under reduced pressure. The crude was purified by silica column chromatography (heptane/EtOAc, 75:25) to afford 187 (0.93 g, 89%) as an orange oil.
To a mixture of 187 (20.0 mg, 60.0 mmol) and N-boc-O-tosylhydroxylamine (26.2 g, 91.2 mmol) in t-BuOH (450 mL), CH3CN (140 mL) and water (105 mL) was added K2OSO4•2H2O (2.21 g, 6.00 mmol). The reaction mixture was stirred at rt for 18 h. The solvent was removed under reduced pressure. The residue was dissolved in EtOAc and washed with NaHCO3 (sat., aq.) and brine. The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford crude 188.
To a solution of 188 (27.0 g, 57.9 mmol) in CH2Cl2 (350 mL) was added TFA (50 mL). The reaction mixture was stirred at rt for 2 h. The mixture was concentrated under reduced pressure. The crude mixture was diluted with CH2Cl2 and the solution was washed with NaHCO3 (sat., aq.). The precipitate was removed by filtration and the filtrate was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was dissolved in EtOAc, dried and evaporated under reduced pressure. The mixture was combined with other fractions (3.47 mmol, 53.6 mmol and 57.9 mmol). The crude was purified by silica column chromatography (CH2Cl2/CH3OH/NH3, 97:3)) to afford the racemic mixture. The enantiomers were separated by SFC (Daicel 300 gr OD-H 5um, mobile phase: 93% CO2, 7% CH3OH + 0.4% i-PrNH2) to afford 189 (13.2 g, 21%); [α]D20 -6.57 (c 1.05, DMF); and 190 (13.6 g, 21%). [α]D20 +7.53 (c 0.425, DMF).
To a mixture of degassed 1,4-dioxane (1.3 L) and water (68 mL) were added methyl 2,6-dichloropyridine-4-carboxylate (25.6 g, 124 mmol), CS2CO3 (61.7 g, 189 mmol), 4-fluorophenylboronic acid (17.0 g, 121 mmol) and PdCl2(dppf) (6.87 g, 9.39 mmol). The reaction mixture was stirred at rt overnight. The mixture was filtered over decalite and washed with EtOAc. The filtrate was concentrated under reduced pressure to afford crude 191 which was used in the next step without further purification.
To a degassed mixture of 1,4-dioxane (1.06 L) and water (56 mL) were added 191 (24.0 g, 55.1 mmol, 61% pure), CS2CO3 (26.0 g, 80.0 mmol), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (12.4 g, 55.6 mmol) and PdCl2(dppf) (3.76 g, 5.14 mmol). The reaction mixture was stirred at 80° C. for 2 days. The mixture was filtered over decalite and washed with EtOAc. The filtrate was concentrated under reduced pressure. The reaction was not finished, so the crude was re-engaged into the reaction. Degassed 1,4-dioxane and water were added, followed by CS2CO3 (26.0 g, 80.0 mmol), 4,4,6-trimethyl-2-(3,3,3-trifluoroprop-1-en-2-yl)-1,3,2-dioxaborinane (12.4 g, 55.6 mmol) and PdCl2(dppf) (3.76 g, 5.14 mmol). The reaction mixture was stirred at 80° C. for 2 days. The mixture was filtered over decalite and washed with EtOAc. The filtrate was concentrated under reduced pressure. The crude was purified by silica column chromatography (heptane/CH2Cl2, gradient from 70:30 to 30:70) to afford 192 (18.8 g, 38% over 2 steps, 95% pure).
A solution of 192 (15.7 g, 45.9 mmol, 95% pure) in THF (400 mL) was stirred under N2 atmosphere and cooled to 0° C. CH3MgBr (3.4 M in 2-methylTHF, 40.5 mL, 138 mmol) was added dropwise and the reaction mixture was stirred for 1 h at 0° C., then at rt for 2 h. The mixture was carefully diluted with EtOAc and NH4Cl (aq.) was added. The layers were separated, and the aqueous phase was extracted with EtOAc. The combined organic extracted were washed with brine and dried (MgSO4). The solids were removed by filtration and the filtrate was evaporated under reduced pressure. The residue was purified over a silica column (heptane/CH2Cl2, gradient from 1:1 to 0:1) to afford 193 (13.8 g, 93%).
A solution of 193 (13.8 g, 42.42 mmol) and A-boc-O-tosylhydroxylamine (24.4 g, 84.8 mmol) in t-BuOH (360 mL) and water (40 mL) was stirred at rt. K2OSO4•2H2O (0.78 g, 2.12 mmol) was added and the reaction mixture was stirred at rt overnight. The mixture was concentrated under reduced pressure. the residue was dissolved in CH2Cl2 and the solution was washed with water. The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was evaporated under reduced pressure. The crude mixture was purified over a silica column (CH2Cl2) to afford 194 (19.4 g, quant.).
A solution of 194 (19.3 g, 42.0 mmol) in 1,4-dioxane (250 mL) and ethanol (25 mL) was stirred at rt. HCl (4 M in 1,4-dioxane, 52.5 mL, 210 mmol) was added dropwise and the reaction mixture was stirred overnight at rt. The mixture was evaporated under reduced pressure and diluted with ice water. The solution was alkalized with Na2CO3 (sat., aq.). the aqueous phase was extracted with CH2Cl2 (twice). The combined organic extracted were dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The residue was triturated in DIPE and CH3CN (9:1). The solid was collected by filtration and dried under vacuum to afford a mixture of enantiomers (11.3 g). The enantiomers were separated by SFC (stationary phase: Chiralpak Daicel IG 20 x 250 mm, mobile phase: CO2, EtOH + 0.4% i-PrNH2) to afford 195 (5.6 g, 37%) LC-MS (method C): Rt = 1.78 min; mass calcd. for C17H18F4N2O2 358.1, m/z found 359.1 [M+H]+and 196 (5.7 g, 38%) LC-MS (method C): Rt = 1.78 min; mass calcd. for C17H18F4N2O2 358.1, m/z found 359.2 [M+H]+.
2-bromo-5-fluoroisonicotinic acid (10.0 g, 45.5 mmol), (4-fluorophenyl)boronic acid (7.63 g, 54.5 mmol), CS2CO3 (44.4 g, 136 mmol) were suspended in H2O (60 mL) and 1,4-dioxane (240 mL). The mixture was sparged with Ar for 5 minutes and then treated with Pd(dppf)Cl2 (3.33 g, 4.55 mmol). The mixture was sparged with Ar for another 5 min and then stirred at 100° C. for 16 hours. The reaction mixture was filtered through a pad of Celite® and the pad washed with ethyl acetate (200 mL) and H2O (120 mL). The layers were separated and the aqueous layer was adjusted to pH = 4 with 1 N HCl. The suspension isolated via filtration. The filter cake was washed with H2O (50 mL X 3) before drying under reduced pressure to afford 5-fluoro-2-(4-fluorophenyl)isonicotinic acid (9.0 g, 82%).
Thionyl chloride (11.7 mL, 160 mmol) was added to a solution consisting of 5-fluoro-2-(4-fluorophenyl)isonicotinic acid (9.0 g, 38 mmol) and ethanol (100 mL). The resultant mixture was refluxed at 80° C. for 1 hour. The reaction mixture was concentrated to dryness under reduced pressure to give the crude product. The residue was extracted with ethyl acetate (60 mL *2) after adding a solution sat. NaHCO3 (60 mL). The combined organic extracts were dried over anhydrous Na2SO4, filtered, and concentrated to dryness under reduced pressure to give the crude product, which was purified by column chromatography (eluent: petroleum ether: ethyl acetate = 10:1) to afford ethyl 5-fluoro-2-(4-fluorophenyl)isonicotinate (9.2 g, 91%) as a colourless oil.
H2O2 (21.0 mL, 209 mmol, 30% purity) was added dropwise to a solution consisting of ethyl 5-fluoro-2-(4-fluorophenyl)isonicotinate (7.0 g, 27 mmol) and TFAA (50 mL) at 0° C. The resultant mixture was stirred at 100° C. for 2 hours. The reaction mixture was poured into sat.Na2SO3 (aq, 150 mL), extracted with ethyl acetate (150 mLx 3).
The combined organic extracts were dried over anhydrous Na2SO4, filtered, and concentrated to dryness under reduced pressure to give the crude product, which was purified by column chromatography (eluent: petroleum ether: ethyl acetate = 10:1 to 4:1) to afford 4-(ethoxycarbonyl)-5-fluoro-2-(4-fluorophenyl)pyridine 1-oxide (4.5 g, 60%) as a white solid and recovered starting material ethyl 5-fluoro-2-(4-fluorophenyl)isonicotinate (4.0 g, 56%).
A solution consisting of 4-(ethoxycarbonyl)-5-fluoro-2-(4-fluorophenyl)pyridine 1-oxide (4.0 g, 14 mmol) and POCl3 (25 mL) was stirred at 110° C. for 2 hours. Then about 15 mL of phosphorus oxychloride was removed by distillation.
The resulting mixture was cooled to room-temperature and dropped-wise into water (70 mL) and stirred for 15 min. Saturated ammonium hydroxide solution (about 20 mL) was added to adjust the pH to 7. The resultant mixture was extracted with ethyl acetate (80 mL x 2). The combined organic extracts were dried over anhydrous Na2SO4, filtered, and concentrated to dryness under reduced pressure to give the crude product, which was purified by column chromatography (eluent: petroleum ether: ethyl acetate = 10:1 to 4:1) to afford the 4-(ethoxycarbonyl)-5-fluoro-2-(4-fluorophenyl)pyridine 1-oxide (4.2 g, 90%) as a white solid.
Methyl magnesium bromide (18 mL, 54 mmol, 3 M in Et2O) was added to a solution consisting of 4-(ethoxycarbonyl)-5-fluoro-2-(4-fluorophenyl)pyridine 1-oxide (4.0 g, 13 mmol) in dry THF (30 mL) at -70° C. (dry ice/ethanol). After addition, the reaction mixture was allowed to warm to room-temperature for 3 hours.The reaction mixture was poured into sat. NH4Cl (100 mL) and extracted with ethyl acetate (100 mL x 2). The combined organic extracts were dried over anhydrous Na2SO4, filtered, and concentrated to dryness under reduced pressure to give the crude product, which was purified by column chromatography (eluent: petroleum ether: ethyl acetate = 10:1) to afford 2-(2-chloro-3-fluoro-6-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (4.0 g, 97%) as a light-yellow solid.
3-amino-1,1,1-trifluoro-2-(3-fluoro-6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)propan-2-ol has been obtained from 2-(2-chloro-3-fluoro-6-(4-fluorophenyl)pyridin-4-yl)propan-2-ol following a similar route to the one described for synthetizing compound 81 and 82.
(-)-3-amino-1,1,1-trifluoro-2-(5-fluoro-4-(2-hydroxypropan-2-yl)-6-(4-(trifluoromethyl)phenyl)pyridin-2-yl)propan-2-ol has been obtained from compound 113 and 4-(trifluoromethyl)phenylboronic acid following a similar route to the one described for synthetizing compound 114
(-)-3-amino-2-(6-(4-chlorophenyl)-5-fluoro-4-(2-hydroxypropan-2-yl)pyridin-2-yl)-1,1,1-trifluoropropan-2-ol has been obtained from compound 113 and 4-chloro phenylboronic acid following a similar route to the one described for synthetizing compound 114
Into a 250 mL Erlenmeyer flask was placed 200 mg of the amine, 100 mL methanol, and approx. 54 mg of 10% Pd/C under nitrogen. The mixture stirred under hydrogen atmosphere for 24h. LCMS shows full conversion to product. The reaction mixture was filtered thought celite, and the solvent was removed under reduced pressure to afford a colourless oil (-)-3-amino-2-(6-phenyl)-5-fluoro-4-(2-hydroxypropan-2-yl)pyridin-2-yl)-1,1,1-trifluoropropan-2-ol (150 mg, 82% yield) which was used as such in the next step.
To a solution of 2,6-dibromopyridine-4-carboxylic acid (50 g, 178 mmol) in methanol (250 mL) H2SO4 (1 mL, 18.76 mmol) was added dropwise and the mixture was refluxed overnight. The mixture was evaporated, taken up in water, neutralised with sodium bicarbonate and three times extracted with dichloromethane. The organic layer was once washed with water, dried over MgSO4, filtered and evaporated to afford 2,6-dibromopyridine-4-carboxylate (49.9 g , 95%)
A mixture of methyl 2,6-dibromopyridine-4-carboxylate (14.7 g, 50 mmol), 4-fluorophenylboronic acid (7 g, 50 mmol), K2CO3 (27.6 g, 200 mmol) and tetrabutylammonium bromide (1.63 g, 5 mmol) was stirred in 1,4-dioxane (150 mL) and water (50 mL). Degassing with nitrogen was done for fifteen minutes and then [1,1′-Bis-(diphenylphosphino)ferrocene] dichloropalladium (II) (740 mg, 0.1 mmol) was added to the mixture. The vessel was closed and stirred at 50° C. for one hour and overnight at ambient temperature. The mixture was evaporated and the residue was diluted with ice water, extracted two times with ethylacetate and the organic layer was dried over MgSO4, filtered and evaporated again. The residue was purified over silica gel column chromarography. with heptane/dichloromethane 100/0 to 50/50 as gradient. The corresponding fractions were evaporated. The first residue was triturated in diisopropylether. The white precipitate was collected by filtration and dried in vacuo (3 g, 18% yield). This filtrate was evaporated together with the second impure part that came from column. This fraction was further purified by Prep HPLC yielding to 6.2 g (39% yield) of the desired compound methyl 2-bromo-6-(4-fluorophenyl)isonicotinate.
To a solution of methyl 2-bromo-6-(4-fluorophenyl)isonicotinate (4.6 g, 14.8 mmol) in 1,4-dioxane (40 mL), MeOH (10 mL) under N2 atmosphere was added 2-(1-cyclopropylvinyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (2.9 g, 14.8 mmol), K2CO3 (4.1 g, 29.7 mmol) and Pd(PPh3)4 (343 mg, 0.3 mmol). The mixture was stirred at 60° C. overnight in a closed vessel. After cooling the mixture was diluted with ethylacetate and twice washed with brine. The organic layer was dried over MgSO4, filtered and evaporated. The residue was purified over silica column chromatography with heptane/dichloromethane 1/0 to 1/1 as a gradient. The corresponding fractions were evaporated to afford methyl 2-(1-cyclopropylvinyl)-6-(4-fluorophenyl)isonicotinate (3.3 g, 74% yield)
(tert-butoxycarbonylamino) 4-methylbenzenesulfonate (6.4 g, 22.2 mmol) was added to a solution of methyl 2-(1-cyclopropylvinyl)-6-(4-fluorophenyl)isonicotinate (3.3 g, 11.1 mmol) in t-BuOH (50 mL) and water, distilled (5 mL). After this potassium osmate (VI) dihydrate (82 mg, 0.22 mmol) was added to the solution. The solution was allowed to stir overnight at room temperature. The mixture was diluted with ethyl acetate and the solution was washed with water and brine. The organic layer was dried with MgSO4, filtered and evaporated.
A purification was performed via Prep HPLC (Stationary phase: RP XBridge Prep C18 OBD-10 µm,50×150 mm, Mobile phase: 0.25% NH4HCO3 solution in water, CH3CN) yielding to methyl 2-(1-cyclopropylvinyl)-6-(4-fluorophenyl)isonicotinate (1.4 g, 29% yield). A solution of methyl 2-(1-cyclopropylvinyl)-6-(4-fluorophenyl)isonicotinate (1.4 g, 3.25 mmol) in THF (30 mL) was stirred under nitrogen and cooled on an ice ethanol bath.
Methylmagnesiumbromide (2.87 mL, 3.4 M, 9.77 mmol) was added dropwise and stirring was done for one hour on the cooling bath and overnight at room temperature. The mixture was carefully diluted with ethylacetate and then decomposed with NH4Cl solution in water and ice. The layers were separated and the waterlayer was extracted one more time with ethylacetate. The combined organic layers were once washed with brine, dried over MgSO4, filtered and evaporated.
A purification was performed via Prep HPLC (Stationary phase: RP XBridge Prep C18 OBD-10 µm,50×150 mm, Mobile phase: 0.25% NH4HCO3 solution in water, CH3CN) yielding tert-butyl (2-cyclopropyl-2-(6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)-2-hydroxyethyl)carbamate (558 mg, yield 39% yield).
A solution of tert-butyl (2-cyclopropyl-2-(6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)-2-hydroxyethyl)carbamate (558 mg, 1.3 mmol) in 1,4-dioxane (5 mL) and ethanol (5 mL) was stirred at room temperature. HCl (4 M in dioxane) (3.24 mL, 4 M, 12.96 mmol) was added dropwise and stirring was continued overnight at ambient temperature. The mixture was evaporated, dissolved in water, alkalised with Na2CO3 solution and two times extracted with dichloromethane. The organic layer was dried over MgSO4, filtered and evaporated. The residue was purified over a RediSep column with dichloromethane/methanol-NH3 98/2 to 95/5 as gradient. The corresponding fractions were evaporated to afford 2-(2-(2-amino-1-cyclopropyl-1-hydroxyethyl)-6-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (430 mg, 100% yield)
The enantiomers were separated by Prep SFC (Stationary phase: Chiralpak Daicel IC 20 x 250 mm, Mobile phase: CO2, EtOH—iPrOH (50-50) + 0.4% iPrNH2) yielding to (-)-2-(2-(2-amino-1-cyclopropyl-1-hydroxyethyl)-6-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (167 mg , 39% yield) and (+)-2-(2-(2-amino-1-cyclopropyl-1-hydroxyethyl)-6-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (193 mg, 45% yield) .
To a solution of 1-fluorocyclopropanecarboxylic acid (10 g, 96.1 mmol) in dry DCM (450 mL) was added dry Et3N (41.7 mL, 0.7 g/mL, 288.2 mmol) and the mixture was stirred for 2 min. Then solid N,O-dimethylhydroxylamine hydrochloride (11.2 g, 115.3 mmol) was added and the mixture was stirred for 2 min. Finally, 1-Propanephosphonic anhydride (50 W/W% solution in EtOAc) (111 mL, 1.1 g/mL, 191.9 mmol) was added dropwise and the reaction mixture was stirred at rt for 16 h. The RM was poured out in sat NaHCO3 solution (500 mL), the organic layer was separated, the aqueous phase was extracted two times more with DCM. The combined organic layers were dried over MgSO4 and evaporated, yielding 1-fluoro-N-methoxy-N-methylcyclopropane-1-carboxamide (13.8 g, yield 98%) as a yellow oil.
To a solution of 2-fluoro-2-methylpropanoic acid (10 g, 94 mmol) in dichloromethane (500 mL) was added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (21 g, 113 mmol), 1-hydroxybenzotriazole (15.2 g, 113 mmol,) and triethylamine (47.7 g, 471 mmol). N,O-dimethylhydroxylamine hydrochloride (11 g, 122 mmol) was added into the mixture. The reaction mixture was stirred at r.t. for 16 h. cooling down to r.t., the resulting mixture was filtered. The filtrate was washed with 2 M HCl solution(3 x 200 mL). Then the mixture was washed with a saturated sodium bicarbonate solution (3 x 200 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated to afford the 2-fluoro-N-methoxy-N,2-dimethylpropanamide crude 2-fluoro-N-methoxy-N,2-dimethylpropanamide as a yellow oil (10.6 g, 75% yield).
2-Fluoro-N-methoxy-N-methylcyclopropane-1-carboxamide have been prepared following a similar route to the one described for synthetizing 2-fluoro-N-methoxy-N,2-dimethyl-propanamide crude 2-fluoro-N-methoxy-N,2-dimethylpropanamide starting from 2-fluorocyclopropane-1-carboxylic acid.
To a solution of 2-(2-chloro-3-fluoropyridin-4-yl)propan-2-ol (49 g, 258 mmol) in dichloromethane (800 mL) was added triethylamine (65 g, 646 mmol,) and trifluoromethanesulfonic acid tert-butyldimethylsilyl ester (102 g, 387 mmol,). Then the reaction mixture was stirred at 45° C. for 16 h. After cooling down to r.t., the reaction was quenched with water (600 mL). The resulting mixture was extracted with dichloromethane (3 x 800 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue obtained was purified by column chromatography (0-20% ethyl acetate/petroleum ether) to afford 4-(2-(tert-butyldimethylsilyloxy)propan-2-yl)-2-chloro-3-as a yellow oil (59 g, 75 yield).
A solution of 2-(dimethylamino)ethanol (17.6 g, 197 mmol) in hexane (500 mL) was cooled at -5° C. and n-BuLi (157 mL, 395 mmol) was added dropwise under a nitrogen atmosphere. After 30 min at 0° C. , the solution was cooled at -78° C. and a solution of 4-(2-(tert-butyldimethylsilyloxy)propan-2-yl)-2-chloro-3-fluoropyridine (20 g, 65.8 mmol) in hexane (60 mL) was added dropwise. After 1 h of stirring a deep rust coloured solution was observed. Then a solution of 1-fluoro-N-methoxy-N-methylcyclopropane-1-carboxamide (38.8 g, 263 mmol) in THF (100 mL) was introduced dropwise. After addition, the reaction medium was allowed to warm slowly to r.t. (1 h). The mixture was quenched by water (1000 mL) and extracted by EtOAc (500 mL x 3). The organic phase was dried over Na2SO4 and concentrated under reduced pressure. The crude product was purified by silica gel chromatography (petroleum ether : EtOAc ═ 50 : 1) to afford (4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-6-chloro-5-fluoropyridin-2-yl)(1-fluorocyclopropyl)methanone ( 18 g, yield:70% ) as a yellow oil.
Trimethylsulfoxonium iodide ( 11.2 g, 50.8 mmol) was added to the solution of Potassium tert-butoxide (5.7 g, 50.8 mmol) in DMSO (300 mL). The reaction mixture was stirred at rt for 0.5 h. Then (4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-6-chloro-5-fluoropyridin-2-yl)(1-fluorocyclopropyl)methanone (18 g, 46 mmol) was added to the mixture and stirred another 2 h. The mixture was added with water (200 mL) and extracted by EtOAc (300 mL x 3).The organic phase was dried over Na2SO4 and concentrated under reduced pressure to afford 4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-2-chloro-3-fluoro-6-(2-(1-fluorocyclopropyl)oxiran-2-yl)pyridine ( 18 g, yield : 97% ) as a yellow oil.
4-((Tert-butyldimethylsilyl)oxy)propan-2-yl)-2-chloro-3-fluoro-6-(1-fluorocyclopropyl)oxiran-2-yl)pyridine (18 g, 44.6 mmol) was dissolved in NH3 in MeOH (300 mL). The mixture was stirred at 35° C. for 16 h. The mixture was concentrated under reduced pressure. The crude product was purified by silica gel chromatography (petroleum ether : EtOAc = 10 : 1) to afford 2-amino-1-(4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-6-chloro-5-fluoropyridin-2-yl)-1-(1-fluorocyclopropyl)ethan-1-ol ( 10 g, yield: 53 % ) as a yellow solid.
Di-tert-butyl dicarbonate ( 4.7 mL, 22.2 mmol), Triethylamine ( 7 mL, 50.5 mmol,) has been added to a solution of 2-amino-1-(4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-6-chloro-5-fluoropyridin-2-yl)-1-(1-fluorocyclopropyl)ethan-1-ol ( 8.5 g, 20.2 mmol), in dichloromethane (100 mL). The mixture was stirred at room temperature for 2 h. The mixture was concentrated under reduced pressure to afford tert-butyl (2-(4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-6-chloro-5-fluoropyridin-2-yl)-2-(1-fluorocyclopropyl)-2-hydroxyethyl)carbamate (10 g, yield: 95%).
Tetrakis(triphenylphosphine)palladium (0.88 g, 0.77 mmol) was added to a solution of tert-butyl (2-(4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-6-chloro-5-fluoropyridin-2-yl)-2-(1-fluorocyclopropyl)-2-hydroxyethyl)carbamate (4 g, 7.7 mmol), (4-fluorophenyl)boronic acid (1.6 g, 11.5 mmol), potassium carbonate (2.65 g, 19.2 mmol) in DME (36 mL) and water (12 mL). The mixture was stirred at 160° C. for 5 min under microwave. The mixture was added with water (50 mL) and extracted by EtOAc (70 mL x 3). The organic phase was dried over Na2SO4 and concentrated under reduced pressure. The crude product was purified by silica gel chromatography (petroleum ether : EtOAc = 50 : 1) to afford tert-butyl (2-(4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-5-fluoro-6-(4-fluorophenyl)pyridin-2-yl)-2-(1-fluorocyclopropyl)-2-hydroxyethyl)carbamate (8 g) as a yellow oil.
Hydrochloric acid (80 mL) has been added to a solution of tert-butyl (2-(4-(2-((tert-butyldimethylsilyl)oxy)propan-2-yl)-5-fluoro-6-(4-fluorophenyl)pyridin-2-yl)-2-(1-fluorocyclopropyl)-2-hydroxyethyl)carbamate (10 g, 17.2 mmol ) in Methanol (40 mL). The mixture was stirred at room temperature for 2 h. Themixture was concentrated under reduced pressure and 40 mL of water was added. The mixture was and extracted by DCM (30 mL x 3). The organic phase was concentrated under reduced pressure to afford 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (6 g, yield : 95%).
The racemates of 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol ( 6.5 g, 17.7 mmol) were separated by Prep-Chiral-HPLC with the following conditions: Column: CHIRAL ART Cellulose-SB S-5um 50*250mm, 50 mm*250 mm 5 um; Mobile Phase A:CO2, Mobile Phase B:MEOH(2 mM NH3-MEOH); Flow rate: 150 mL/min; Gradient:24% B; 220 nm; RT1:4.89; RT2:5.67; Injection Volumn:4 ml; Number Of Runs:38; to afford : (-)2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (first eluting isomer): (2.8 mg, 42 %) as a yellow solid. e.e = 100%. [α] = -6° (589 nm, 23.6° C., 5 mg in 10 mL MeOH), and (+) 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (second eluting isomer): (3.2 g, 45%) as a yellow solid. e.e = 98.44%. [α] = +6° (589 nm, 23.6° C., 5 mg in 10 mL MeOH)
2-(6-(2-Amino-1-(2,2-dimethylcyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)-pyridin-4-yl)propan-2-ol have been prepared following a similar route to the one described for synthetizing 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol using N-methoxy-N,2,2-trimethyl-cyclopropanecarboxamide.
1-Amino-3-fluoro-2-(5-fluoro-6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)-3-methylbutan-2-ol have been prepared following a similar route to the one described for synthetizing 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol using 2-fluoro-N-methoxy-N,2-dimethylpropanamide crude 2-fluoro-N-methoxy-N,2-dimethylpropanamide.
2-(6-(2-Amino-1-hydroxy-1-(1-methylcyclopropyl)ethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol has been prepared following a similar route to the one described for synthetizing 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol using N-methoxy-N,1-dimethylcyclopropane-carboxamide.
2-(6-(2-Amino-1-(2-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol have been prepared following a similar route to the one described for synthetizing 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol using 2-fluoro-N-methoxy-N-methylcyclopropane-1-carboxamide.
1-Amino-3,3-difluoro-2-(5-fluoro-6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)butan-2-ol have been prepared following a similar route to the one described for synthetizing 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol using 2,2-difluoro-N-methoxy-N-methylpropanamide
The racemates of 1-amino-3,3-difluoro-2-(5-fluoro-6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)butan-2-ol (300 mg, 0.806 mmol) was separated by Prep-Chiral-HPLC with the following conditions: Column: Reg-AD Column, 4.6*100 mm 5 um; Mobile Phase A:Hex ( 0.1%DEA): EtOH=90:10 , Mobile Phase B:; Flow rate: 1 mL/min; Gradient:0 B to 0 B in min; nm; RT1:; RT2:; Injection Volumn: ml;
(-)-1Amino-3,3-difluoro-2-(5-fluoro-6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)butan-2-ol1 (first eluting isomer): (113 mg, 37 %) as a white solid. e.e = 99.574%. [α] = -18° (589 nm, 24.2° C., 5 mg in 10 mL MeOH).
(+) 1-amino-3,3-difluoro-2-(5-fluoro-6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)butan-2-ol (second eluting isomer): (119 mg, 39%) as a white solid. e.e = 99.440%. [α] = +18° (589 nm, 24.6° C., 5 mg in 10 mL MeOH)
1-Amino-3,3-difluoro-2-(5-fluoro-6-(4-fluorophenyl)-4-(2-hydroxypropan-2-yl)pyridin-2-yl)butan-2-ol have been prepared following a similar route to the one described for synthetizing 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol starting from 2-(2-chloropyridin-4-yl)propan-2-ol and using 2,2-difluoro-N-methoxy-N-methylpropanamide
(RS)-2-(6-(2-amino-1-((*S)-2,2-difluoro-1-methylcyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol and (RS)-2-(6-(2-amino-1-((*R)-2,2-difluoro-1-methylcyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol have been obtained following a similar route to the one described for synthetizing 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol starting from using 2,2-difluoro-N-methoxy-N-methylcyclopropane-1-carboxamide. Note : A separation of diastereaoisomers has been performed before deprotection of the amino and hydroxy group.
2-(6-(2-amino-1-cyclopropyl-1-hydroxyethyl-2,2-d2)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol have been prepared following a similar route to the one described for synthetizing 2-(6-(2-amino-1-(1-fluorocyclopropyl)-1-hydroxyethyl)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol using N-methoxy-N-methylcyclopropanecarboxamide.
Note : in step 3 : DMSO has been replaced by DMSO-d6, in step 6: water has been replaced by D2O and in step 7, methanol has been replaced by deuterated methanol.
The enantiomers of 2-(6-(2-amino-1-cyclopropyl-1-hydroxyethyl-2,2-d2)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (340 mg, 0.970 mmol) were separated by Prep-Chiral-HPLC with the following conditions: column: Chrialpak AD-H, 2 x 25 cm, 5 um; Mobile Phase A: Hex (8 mmol/L NH3. MeOH )--HPLC, Mobile Phase B: EtOH--HPLC; Flow rate: 20 mL/min; Gradient: 5% B to 5% B in 21 min; 220/254 nm; Rt1: 10.004 min; Rt2: 15.561 min; Injection Volumn: 0.5 ml; Number of Runs: 12; (-)-2-(6-(2-amino-1-cyclopropyl-1-hydroxyethyl-2,2-d2)-3-fluoro-2-(4-fluorophenyl)pyridin-4-yl)propan-2-ol (125.6 mg, 36%) as light yellow solid. [α]= -10° (589 nm, 24.2° C., 5 mg in 10 mL MeOH).
(+)(6-(2-amino-1-cyclopropyl-1-hydroxyethyl-2,2-d2)-3-fluoro(4-fluorophenyl)pyridin-4-yl)propan (114.9 mg, 33%) as light yellow solid. [α]= +10° (589 nm, 24.2° C., 5 mg in 10 mL MeOH).
I2 (5.3 g, 21 mmol), ethyl 8-methoxyquinoline-6-carboxylate (3.0 g, 14 mmol), t-BuOOH (14.0 g, 109 mmol, 70%), and CH3CN (50 mL) was added to a 100 mL round-bottomed flask. The resultant mixture was stirred at 80° C. for 16 hours. The reaction mixture was poured into sat. Na2SO3 (200 mL), extracted with ethyl acetate (100 mL x 3), the combined organic extracts were dried over anhydrous Na2SO4, filtered, and concentrated to dryness under reduced pressure to afford the crude product, which was purified by FCC (eluent: petroleum ether: ethyl acetate = 1:0 to 3:1, petroleum ether: ethyl acetate = 3:1, Rf=0.7) to afford methyl 3-iodo-8-methoxyquinoline-6-carboxylate, LCMS: as a white solid (3.5 g, 73% yield)
Methyl 2-chloro-2,2-difluoroacetate (5.9 g, 41 mmol) was added to a solution consisting of methyl 3-iodo-8-methoxyquinoline-6-carboxylate (3.5 g, 10 mmol), KF (1.2 g, 21 mmol), CuI (3.9 g, 21 mmol) and dimethylacetamide (60 mL). The mixture was stirred at 130° C. for 16 hours before cooling to room-temperature. The reaction mixture was poured into sat. NaCl (200 mL), extracted with ethyl acetate (80 mL x 3), the combined organic extracts were dried over anhydrous Na2SO4, filtered, and concentrated to dryness under reduced pressure to afford the crude product, which was purified by flash column chromatography (eluent: petroleum ether: ethyl acetate = 1:0 to 3:1, petroleum ether: ethyl acetate = 3:1, Rf=0.7) to afford methyl 8-methoxy-3-(trifluoromethyl)quinoline-6-carboxylate as a brown solid (2.5 g, 73% purity, 63% yield)
LiOH.H2O (551 mg, 13.1 mmol) was added into to a 0° C. (ice/water) mixture consisting of methyl 8-methoxy-3-(trifluoromethyl)quinoline-6-carboxylate (2.5 g, 8.8 mmol), THF (15 mL) and H2O (15 mL). The resultant mixture was stirred at room-temperature for 2 hours. The reaction mixture was acidified to pH = 5 by addition ofHCl.
The mixture was concentrated to dryness under reduced pressure to afford the crude product, which was purified by preparative HPLC with a Phenomenex Synergi Max-RP 250*50 mm*10 um (eluent: 25% to 55% (v/v) water (0.1%TFA)-ACN to afford title product. The product was suspended in water (50 mL), the mixture frozen using dry ice/acetone, and then lyophilized to dryness to afford 8-methoxy-3-(trifluoromethyl)quinoline-6-carboxylic acid as a yellow solid (910 mg, 39% yield).
To a cooled (0° C.) solution of 4-bromo-2-fluoro-aniline (22 g, 115.8 mmol) in H2O (250 mL) was treated with HCl (12 M, 28 mL) and NaNO2 (9.6 g, 138.9 mmol) After 40 min at 0° C., HCl (12 M, 30 mL ) and sodium tetrafluoroborate (50.8 g, 463 mmol) was added. After stirring at 0° C. for 40 min, the intermediate diazonium was filtered, A solution of crude product in MeCN (200 mL) was treated with ethyl-3-morpholinoprop-2-enoate (21.4 g, 115 mmol) ,The mixture was stirred at 25° C. for 10 hr. The mixture was concentrated. The residue was purified by column chromatography (SiO2, Petroleum ether/Ethyl acetate=50/1 to 5:1) to afford 2-(4-bromo-2-fluorophenyl)diazenyl)-3-hydroxyacrylate (20 g, 54%) as yellow solid was obtained .
To a solution ethyl 2-(4-bromo-2-fluorophenyl)diazenyl)-3-hydroxyacrylate (12 g ,36.2 mmol) was added H2SO4 (100 mL) stirred at 100° C. for 8 h. The mixture was quenched by water (1000 mL) then extracted with ethyl acetate (800 mL*3) .The organic phase was concentrated under vacuum to give brown solid .The brown solid (8 g ,crude) was used for the next step directly without purification
A solution of 6-bromo-8-fluorocinnoline-3-carboxylic acid (10 g, 37 mmol) in tert-butanol (120 mL) was added diphenyl phosphorazidate (12.2 g, 44.4 mmol) and triethylamine (8.2 g, 81.4 mmol), the mixture was heated at 95° C. for 5 h. After cooling down to rt, the solvent was removed under reduced pressure and the residue obtained was purified by silica gel chromatography (0-10% ethyl acetate/petroleum ether) to afford the tert-butyl (6-bromo-8-fluorocinnolin-3-yl)carbamate yellow solid (3 g, 24% yield).
To a solution of tert-butyl (6-bromo-8-fluorocinnolin-3-yl)carbamate (2.4 g, 7 mmol) in MeOH (50 mL) was treated with NaOMe (1.1 g, 21 mmol) the mixture was stirred at rt for 12 h. The reaction was quenched with water (20 mL). The resulting mixture was extracted with ethyl acetate (3 x 70 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered, and concentrated to provide tert-butyl (6-bromo-8-methoxycinnolin-3-yl)carbamate (0.86 g, 35%) as a yellow solid.
To solution of tert-butyl (6-bromo-8-methoxycinnolin-3-yl)carbamate (0.85 g, 2.4 mmol) in MeOH (20 mL) was treated with 1,1′-bis(diphenylphosphino)ferrocene-palladium(II)dichloride dichloromethane complex (196.0 mg, 0.24 mmol) and Et3N (0.49 g, 4.8 mmol), the mixture was stirred under CO (1 atm) at 50° C. for 1 h. After cooling to r.t., the solvent was removed in vacuo and the residue was purified by silica gel chromatography (0-20% ethyl acetate/petroleum ether) to provide the methyl 3-((tert-butoxycarbonyl)amino)-8-methoxycinnoline-6-carboxylate (630 mg, 79% yield) as a yellow solid.
To a solution of methyl 3-((tert-butoxycarbonyl)amino)-8-methoxycinnoline-6-carboxylate (0.63 g, 1.9 mmol) in DCM (15 mL) was treated with TFA (4.3 g, 38 mmol), the mixture was stirred at rt for 12 h. The solvent was removed under reduced pressure and the crude product was applied onto C18 (5-60% MeCN/H2O(0.05% NH4HCO3)) provided methyl 3-amino-8-methoxycinnoline-6-carboxylate (400 mg, 90 % yield) as a yellow solid.
To a solution of methyl 3-amino-8-methoxycinnoline-6-carboxylate (326.5 mg, 1.4 mmol) in DCM (8 mL) and H2O (8 mL)was treated with CH2I2 (750 mg, 2.8 mmol), NaNO2 (483 mg, 7 mmol) and HOAc (1.68 g, 28 mmol), the mixture was stirred at rt for 12 h. The solvent was removed under reduced pressure and the crude was purified by silica gel chromatography (0-50% ethyl acetate/petroleum ether) to provide methyl 3-iodo-8-methoxycinnoline-6-carboxylate (180 mg, 38% yield) as a yellow solid .
Under a nitrogen atmosphere, benzene (520.5 mg, 6.66 mmol) and Tf2O (844.2 mg, 3.0 mmol) were added into a suspension of TfSO2Na (212.2 mg, 1.4 mmol) in DCM (8 mL), which was well cooled by ice-bath. After stirring at 0° C. for 1.5 h, the reaction mixture was warmed to rt and allowed to react for 34 h. Then the reaction mixture was diluted with DCM, washed successively with saturated aqueous NaHCO3 and NaCl and dried over Na2SO4, the solvent was removed under reduced pressure and get [Ph2SCF3][OTf] as a yellow solid. In a 10 mL sealed tube, methyl 3-iodo-8-methoxycinnoline-6-carboxylate (234 mg, 0.68 mmol) and [Ph2SCF3][OTf] (550 mg,1.36 mmol) were dissolved in DMF (8 mL), Cu (130 mg, 2.04 mmol) was added. The reaction mixture was stirred at 70° C. for 12 h. After cooling down to rt, the solvent was removed under reduced pressure and the crude was purified by silica gel chromatography (0-50% ethyl acetate/petroleum ether) to provide the target product (27 mg, 13% yield) as a yellow solid.
To a solution of methyl 8-methoxy-3-(trifluoromethyl)cinnoline-6-carboxylate (278 mg, 0.97 mmol) in MeOH (10 mL),THF (10 ml) and H2O (2 mL) was treated with NaOH (0.155 g, 3.9 mmol), the reaction mixture was stirred at rt for 2 h. The reaction was neutralized with 1 N HCl (4 mL) and extracted with ethyl acetate (3 X 20 mL), the combined organic layer was washed with brine and dried over anhydrous Na2SO4, the solvent was removed under reduced pressure and the crude product was applied onto C18 (5-60% MeCN/H2O (0.05% HCl)) provide the target product (65.4 mg, 25% yield) as a light yellow solid .
To a solution of methyl 4-amino-3-iodo-5-methoxybenzoate (8 g, 26.051 mmol) in DMF (80 mL) and MeOH (20 mL) was added trimethyl(prop-1-yn-1-yl-d3)silane (6.006 g, 52.103 mmol), CuI (0.992 mg, 5.210 mmol), CsF (11.8 mg, 78 mmol), PdCl2(PPh3)2 (0.91 g, 1.303 mmol). The resulting mixture was maintained under nitrogen and stirred at 30° C. for 30 min. The mixture was filtered through a celite pad and was washed with CH2Cl2 (200 mL X 1). The filtrate was concentrated under reduced pressure to remove MeOH and CH2Cl2. The residue was poured into ice water. The precipitated solid was filtered and was washed with water (200 mL). The solid was dissolved in CH2Cl2 (200 mL).The combined organic layer was washed with saturated brine (200 mL X 6). The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was dried over vacuum to afford methyl 4-amino-3-methoxy-5-(prop-1-yn-1-yl-d3)benzoate as a brown solid (5 g, 87% yield).
Methyl 4-amino-3-methoxy-5-(prop-1-yn-1-yl-d3)benzoate (6 g, 27 mmol, 1 eq) was dissolved in 1-methyl-2-pyrrolidinone (120 mL). The resultant mixture was purged with N2 then potassium tert-butoxide (7.573 g, 67 mmol) was added. The mixture was purged again with N2 then stirred at 30° C. for 4 h. A saturated aqueous solution of NH4Cl was added to the crude. The resulting mixture was extracted with ethyl acetate (3 x 100 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue obtained was purified by silica gel chromatography (0-10% ethyl acetate/petroleum ether) to afford the methyl 7-methoxy-2-(methyl-d3)-1H-indole-5-carboxylate as a yellow solid (3.95 g, 66% yield). Methyl 7-methoxy-2-(methyl-d3)-1H-indole-5-carboxylatee (1.18 g, 5.3 mmol) was dissolved in DMF (30 mL). The resultant mixture was purged with N2. Then potassium tert-butoxide (1.19 g, 10.6 mmol) was added in the mixture. After that O-(4-nitrobenzoyl)hydroxylamine (1.9 g, 10.6 mmol) was added to the mixture. The mixture was purged again with N2 then stirred at r.t. for 4 h. A saturated aqueous solution of NH4Cl was added to the crude. The resulting mixture was extracted with ethyl acetate (3 x 30 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue obtained was purified by silica gel chromatography (0-30% ethyl acetate/petroleum ether) to afford the methyl 1-amino-7-methoxy-2-(methyl-d3)-1H-indole-5-carboxylate as a yellow solid (700 mg, 56 % yield).
To a solution of methyl 1-amino-7-methoxy-2-(methyl-d3)-1H-indole-5-carboxylate (700 mg, 2.950 mmol) in methanol (18 mL) was added 4 M hydrogen chloride solution in methanol (6 mL). The resulting mixture was heated to 80° C. for 16 h. After cooling down to r.t., the mixture was filtrated and concentrated. The mixture was dissolved with dichloromethane. A saturated aqueous solution of NaHCO3 was added to the crude. The organic layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue obtained was purified by silica gel chromatography (0-50% ethyl acetate/petroleum ether) to afford the methyl 8-methoxy-3-(methyl-d3)cinnoline-6-carboxylate as a yellow solid (520 mg, 75% yield).
To a solution of methyl 8-methoxy-3-(methyl-d3)cinnoline-6-carboxylate (510 mg, 2.2 mmol) in methanol (10 mL) and tetrahydrofuran (10 mL) was added sodium hydroxide (346 mg, 8.6 mmol, 4 eq) and D2O (2 mL). The mixture was stirred at 80° C. for 4 h. The mixture was filtrated and concentrated. The residue obtained was purified by flash column chromatography (C18: H2O(0.05%):MeCN=1:1) to afford the 8-methoxy-3-(methyl-d3)cinnoline-6-carboxylic acid as a yellow solid (287.1 mg, 58% yield).
To a solution of methyl 3-cyclopropyl-8-methoxycinnoline-6-carboxylate (5 g, 19.35 mmol) in dichloromethane (80 mL) was added boron tribromide (14.55 g, 58.08 mmol) at 0° C. The mixture was stirred at r.t. for 2 h. The mixture was filtrated and concentrated afford the methyl 3-cyclopropyl-8-hydroxycinnoline-6-carboxylate crude as a yellow solid (4.3 g, 91 % yield).
Methyl 3-cyclopropyl-8-hydroxycinnoline-6-carboxylate (1 g, 4.09 mmol and potassium carbonate (1.69 g, 12.3 mmol) were added into a round bottom flask. The mixture was purged with N2. After that acetonitrile (40 mL) was added. The mixture was purged again with N2 then iodomethane-D3 (2.37 g, 16.37 mmol) was added. The mixture was purged again with N2 then stirred at 40° C. for 2 h. The resulting mixture was extracted with dichloromethane (3 x 50 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue obtained was purified by silica gel chromatography (0-50% ethyl acetate/petroleum ether) to afford the methyl 3-cyclopropyl-8-(methoxy-d3)cinnoline-6-carboxylate as a yellow solid (400 mg, 37% yield).
To a solution of methyl methyl 3-cyclopropyl-8-(methoxy-d3)cinnoline-6-carboxylate (1.07 g, 4.09 mmol) in methanol (8 mL) and tetrahydrofuran (8 mL) was added lithium hydroxide (0.29 g, 12.29 mmol) and H2O (1.6 mL). The mixture was stirred at r.t. for 1 h. The mixture was filtrated and concentrated. The residue obtained was purified by silica gel chromatography (0-50% H2O/ACN) to afford the 3-cyclopropyl-8-(methoxy-d3)cinnoline-6-carboxylic acid as a yellow solid (351 mg, 70.767 % yield).
8-(Methoxy-d3)-3-methylcinnoline-6-carboxylic acid has been prepared following a similar procedure to the one use to make 3-cyclopropyl-8-(methoxy-d3)cinnoline-6-carboxylic acid
The final compounds were synthesized according to one of the following procedures: A, B, C or D.
In a vial, to a mixture of amine (1 equiv.) and carboxylic acid (1.5 equiv.) in anhydrous DMF (0.027 M) was added Et3N (3 equiv.) followed by DEPC (2 equiv.) The vial was sealed and stirred at rt for 16 h. The solution was then submitted for purification.
To a mixture of acid carboxylic (1 equiv.) and amine (1 equiv.) in DMF (0.17 M) was added DIPEA (2 equiv.) followed by HATU (1 equiv.) portionwise. The reaction mixture was stirred at rt for few hours. The reaction was diluted with water and stirred at rt for another 2 h. The precipitate was collected by filtration and dried under vacuum. Then a purification was eventually performed.
To a mixture of carboxylic acid (1 equiv.) and amine (1.1 equiv) in anhydrous DMF (0.10 M) were added HATU (1.5 equiv.) and DIPEA (2 equiv.). The reaction mixture was stirred at rt for few hours. The mixture was diluted with EtOAc and NaHCO3 (10%, aq.). The layers were separated, and the aqueous phase was extracted with EtOAc (twice). The combined organic extracts were washed with brine (3 times) and dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. Purification of the crude mixture delivered the desired compound.
A mixture of carboxylic acid (1 equiv.), amine (1 equiv.), EDC (1 equiv.), HOBt.H2O (1 equiv.) and DIPEA (2 equiv.) in DMF (0.05 M) was stirred at rt for few hours. The reaction was diluted with H2O, brine, NaHCO3 (sat., aq.) and EtOAc. The layers were separated, and the aqueous phase was extracted with EtOAc. The combined organic extracts were washed with a mixture of KHSO4 and brine (1:1), then with brine (4 times). The organic layer was dried (MgSO4). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. Purification of the crude mixture delivered the desired compound.
200 (121 mg. 80%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.58 (s, 3H), 1.65 (s, 3H), 2.49 (br s, 3H), 3.96 (s, 3H), 4.11 (dd, J=14.1, 5.5 Hz, 1H), 4.22 (dd, J=14.1, 6.2 Hz, 1H), 5.70 (s, 1H), 7.26 - 7.34 (m, 2H), 7.35 (d, J=1.5 Hz, 1H), 7.44 (s, 1H), 7.68 - 7.74 (m, 2H), 7.78 (d, J=1.5 Hz, 1H), 8.06 (dd, J=2.1, 1.0 Hz, 1H), 8.34 (s, 1H), 8.73 (t, J=5.9 Hz, 1H), 8.77 (d, J=2.0 Hz, 1H); LC-MS (method B): Rt = 1.12 min; mass calcd. for C29H26ClF4N3O4 591.0, m/z found 592.3 [M+H]+; [α]D20 -62.55° (c 0.267, DMF).
201 (74 mg. 49%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/ CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.61 (s, 6H), 2.46 (s, 3H), 3.90 (s, 3H), 4.07 (br dd, J=14.0, 5.2 Hz, 1H), 4.41 (br dd, J=13.8, 6.7 Hz, 1H), 5.61 (s, 1H), 7.07 (s, 1H), 7.27 (d, J=1.5 Hz, 1H), 7.30 - 7.42 (m, 2H), 7.72 (d, J=1.5 Hz, 1H), 7.93 (dd, J=7.5, 5.5 Hz, 2H), 7.99 (s, 1H), 8.57 (br t, J=5.9 Hz, 1H), 8.74 (d, J=2.0 Hz, 1H); LC-MS (method B): Rt 1.04 min; mass calcd. for C29H25F6N3O4 593.0, m/z found 594.0 [M+H]+; [α]D20 -25.43° (c 0.260, DMF).
202 (130 mg. 88%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: 250 g, YMC Tri-Art, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 65:35 to 35:65). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.65 (s, 3H), 1.66 (s, 3H), 2.48 (s, 3H), 3.93 (s, 3H), 4.03 (br dd, J=13.9, 5.1 Hz, 1H), 4.41 (br dd, J=14.0, 6.7 Hz, 1H), 5.60 (s, 1H), 6.97 (br s, 1H), 7.24 - 7.31 (m, 3H), 7.53 - 7.61 (m, 2H), 7.71 (d, J=1.5 Hz, 1H), 8.04 (d, J=1.1 Hz, 1H), 8.57 (t, J=6.1 Hz, 1H), 8.76 (d, J=2.2 Hz, 1H); LC-MS (method G): Rt 2.05 min; mass calcd. for C29H25ClF5N3O4 609.2, m/z found 610.2 [M+H]+; [α]D20 -42.09° (c 0.269, DMF).
203 (174 mg. 75%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.46 (s, 3H), 1.54 (s, 3H), 2.48 (s, 3H), 3.82 (br dd, J=13.8, 5.4 Hz, 1H), 3.93 (s, 3H), 3.95 - 4.06 (m, 1H), 5.62 (s, 1H), 6.58 (s, 1H), 6.64 (t, J=54.6 Hz, 1H), 7.22 - 7.32 (m, 3H), 7.72 (d, J=1.5 Hz, 1H), 7.90 - 7.99 (m, 3H), 8.04 (d, J=5.5 Hz, 1H), 8.54 (t, J=6.1 Hz, 1H), 8.75 (d, J=2.2 Hz, 1H); LC-MS (method B): Rt 1.00 min; mass calcd. for C29H27F4N3O4 557.0, m/z found 558.0 [M+H]+. A second purification was performed via normal phase HPLC (stationary phase: Daicel Chiralpak IC, 250 g, 5 µm, mobile phase: heptane/EtOH, 90: 10) to afford 203 (37 mg, 16%); [α]D20 +78.54° (c 0.261, DMF); and 204 (20 mg, 9%).
205 (115 mg. 74%) was synthesized according to procedure A. A purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.18 (m, 1H), 0.26 - 0.33 (m, 1H), 0.34 - 0.42 (m, 1H), 0.52 - 0.60 (m, 1H), 1.46 - 1.53 (m, 1H), 1.56 (s, 3H), 1.64 (s, 3H), 3.34 - 3.40 (m, 3H), 3.79 - 3.86 (m, 1H), 3.89 - 3.99 (m, 1H), 3.96 (s, 3H), 5.56 (br s, 1H), 5.57 (br s, 1H), 7.23 - 7.30 (m, 2H), 7.34 (d, J=1.5 Hz, 1H), 7.66 - 7.72 (m, 2H), 7.74 (d, J=1.5 Hz, 1H), 8.02 (dd, J=2.0, 0.9 Hz, 1H), 8.15 (s, 1H), 8.57 (t, J=5.9 Hz, 1H), 8.76 (d, J=2.0 Hz, 1H); LC-MS (method F): Rt = 2.51 min; mass calcd. for C31H31ClFN3O4 563.0, m/z found 564.0 [M+H]+; [α]D20 -97.84° (c 0.254, DMF).
206 (123 mg. 78%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.11 - 0.19 (m, 1H), 0.26 - 0.34 (m, 1H), 0.36 - 0.45 (m, 1H), 0.54 - 0.62 (m, 1H), 1.48 (s, 3H), 1.54 (s, 3H), 1.55 - 1.60 (m, 1H), 2.48 (s, 3H), 3.88 (dd, J=13.4, 55 Hz, 1H), 3.92 - 3.99 (m, 1H), 3.95 (s, 3H), 5.56 (s, 2H), 7.28 - 7.37 (m, 3H), 7.76 (d, J=1.8 Hz, 1H), 7.94 (d, J=5.5 Hz, 1H), 7.96 - 8.02 (m, 3H), 8.55 (t, J=5.9 Hz, 1H), 8.76 (d, J=2.0 Hz, 1H); LC-MS (method F): Rt = 2.50 min; mass calcd. for C31H31F2N3O4 547.0, m/z found 548.4 [M+H]+; [α]D20 -32.65° (c 0.269, DMF)
207 (141 mg. 47%) was synthesized according to procedure B. The precipitate was purified via silica column chromatography (CH2Cl2/CH3OH, gradient from 99:1 to 95:5). The residue was crystallized from DIPE and CH3CN (10:1) and the precipitate was collected by filtration and dried under vacuum. 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.21 (m, 1H), 0.27 - 0.36 (m, 1H), 0.37 - 0.46 (m, 1H), 0.54 - 0.64 (m, 1H), 1.48 (s, 3H), 1.55 (s, 3H), 1.56 - 1.63 (m, 1H), 2.47 (s, 3H), 3.90 (dd, J=13.4, 5.3 Hz, 1H), 3.95 (s, 3H), 3.95 - 4.00 (m, 1H), 5.58 (s, 1H), 5.60 (s, 1H), 7.35 (d, J=1.6 Hz, 1H), 7.78 (d, J=1.6 Hz, 1H), 7.85 (d, J=8.5 Hz, 2H), 8.01 (d, J=5.7 Hz, 1H), 8.04 (dd, J=2.0, 0.8 Hz, 1H), 8.15 (d, J=8.1 Hz, 2H), 8.55 (t, J=5.9 Hz, 1H), 8.76 (d, J=2.0 Hz, 1H); LC-MS (method H): Rt = 2.13 min; mass calcd. for C32H31F4N3O4 597.2, m/z found 598.5 [M+H]+; [α]D20 -41.28° (c 0.312, DMF).
208 (220 mg. 78%) was synthesized according to procedure B. A purification was performed via SFC (stationary phase: Chiralpak Daicel IC 20 x 250 mm, mobile phase: CO2, EtOH + 0.4% i-PrNH2). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.76 (d, J=2.0 Hz, 1H), 8.56 (t, J=5.9 Hz, 1H), 7.95 - 8.02 (m, 4H), 7.77 (d, J=1.6 Hz, 1H), 7.53 - 7.59 (m, 2H), 7.35 (d, J=1.6 Hz, 1H), 5.57 (s, 1H), 5.56 (s, 1H), 3.85 - 4.00 (m, 2H), 3.95 (s, 3H), 2.48 (s, 3H), 1.56 - 1.62 (m, 1H), 1.54 (s, 3H), 1.48 (s, 3H), 0.54 - 0.62 (m, 1H), 0.37 - 0.46 (m, 1H), 0.27 - 0.35 (m, 1H), 0.11 - 0.20 (m, 1H); LC-MS (method C): Rt = 2.16 min; mass calcd. for C31H31ClFN3O4 563.2, m/z found 564.2 [M+H]+; [α]D20 -47.5° (c 0.53, DMF).
209 (121 mg. 43%) was synthesized according to procedure B. The precipitate was dissolved in CH2Cl2 and the solution was washed with water (twice) and dried (MgSO4). The solids were removed by filtration and the filtrate was evaporated under reduced pressure. The residue was crystallized from DIPE and CH3CN (5:1) and the white precipitate was collected by filtration and dried under vacuum. 1H NMR (400 MHz, DMSO-d6) δ ppm 0.11 - 0.20 (m, 1H), 0.26 - 0.34 (m, 1H), 0.37 - 0.46 (m, 1H), 0.55 - 0.63 (m, 1H), 1.47 (s, 3H), 1.54 (s, 3H), 1.55 - 1.60 (m, 1H), 2.48 (s, 3H), 3.87 (dd, J=13.4, 5.3 Hz, 1H), 3.95 (s, 3H), 3.99 (dd, J=13.4, 6.5 Hz, 1H), 5.57 (s, 1H), 5.58 (s, 1H), 7.34 (d, J=1.6 Hz, 1H), 7.51 - 7.59 (m, 1H), 7.76 (d, J=1.6 Hz, 1H), 7.78 -7.83 (m, 1H), 7.95 - 8.04 (m, 3H), 8.53 (t, J=5.9 Hz, 1H), 8.76 (d, J=2.4 Hz, 1H); LC-MS (method H): Rt = 2.08 min; mass calcd. for C31H30F3N3O4 565.2, m/z found 566.4 [M+H]+; [α]D20 -41.25° (c 0.414, DMF).
210 (117 mg. 75%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.57 (s, 3H), 1.65 (s, 3H), 2.41 (s, 3H), 3.96 (s, 3H), 4.12 (dd, J=14.0, 5.6 Hz, 1H), 4.21 (dd, J=13.8, 6.3 Hz, 1H), 5.70 (s, 1H), 7.26 - 7.35 (m, 2H), 7.39 - 7.46 (m, 2H), 7.66 -7.75 (m, 2H), 7.86 (d, J=1.5 Hz, 1H), 8.30 - 8.36 (m, 2H), 8.75 (t, J=5.8 Hz, 1H); LC-MS (method B): Rt = 1.20 min; mass calcd. for C29H25ClF5N3O4 609.0, m/z found 610.3 [M+H]+; [α]D20 -73.01° (c 0.257, DMF).
211 (77 mg. 50%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.61 (s, 6H), 2.38 (s, 3H), 3.90 (s, 3H), 4.08 (dd, J=14.0, 5.2 Hz, 1H), 4.40 (dd, J=13.9, 6.8 Hz, 1H), 5.60 (s, 1H), 7.04 (s, 1H), 7.30 - 7.39 (m, 3H), 7.80 (d, J=1.5 Hz, 1H), 7.92 (dd, J=7.5, 5.5 Hz, 2H), 8.26 (d, J=10.1 Hz, 1H), 8.58 (br t, J=5.9 Hz, 1H); LC-MS (method B): Rt 1.12 min; mass calcd. for C29H24F7N3O4 611.0, m/z found 612.0 [M+H]+; [α]D20 = -21.58° (c 0.260, DMF).
212 (120 mg. 78%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: 250 g, YMC Tri-Art, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 65:35 to 35:65). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.65(s, 3H), 1.66 (s, 3H), 2.40 (s, 3H), 3.92 (s, 3H), 4.04 (dd, J=14.0, 5.0 Hz, 1H), 4.40 (dd, J=13.8, 6.7 Hz, 1H), 5.53 (s, 1H), 6.95 (s, 1H), 7.22 - 7.33 (m, 2H), 7.35 (d, J=1.3 Hz, 1H), 7.53 - 7.62 (m, 2H), 7.81 (d, J=1.5 Hz, 1H), 8.31 (d, J=9.9 Hz, 1H), 8.59 (br t, J=5.9 Hz, 1H); LC-MS (method G): Rt 2.16 min; mass calcd. for C29H24ClF6N3O4 627.2, m/z found 628.2 [M+H]+; [α]D20 -47.06° (c 0.264, DMF).
213 (56 mg. 31%) and 214 (50 mg, 28%) were synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.58 (t, J=6.2 Hz, 1H), 8.20 (d, J=10.1 Hz, 1H), 8.03 (d, J=5.5 Hz, 1H), 7.93 (dd, J=7.6, 5.6 Hz, 2H), 7.80 (d, J=1.5 Hz, 1H), 7.38 (d, J=1.5 Hz, 1H), 7.20 - 7.30 (m, 2H), 6.63 (t, J=54.6 Hs, 1H), 6.59 (s, 1H), 5.61 (s, 1H), 3.95 - 4.04 (m, 1H), 3.92 (s, 3H), 3.78 - 3.87 (m, 1H), 2.40 (s, 3H), 1.53 (s, 3H), 1.45 (s, 3H): LC-MS (method B): Rt 1.08 min; mass calcd. for C29H26F5N3O4 575.0, m/z found 573.3 [M+H]+. A second purification was performed via silica column chromatography (heptane/EtOH, 90:10) delivered 213 (56 mg, 31%); [α]D20 +83.74° (c 0.268, DMF); and 214 (50 mg, 28%); [α]D20 -77.37° (c 0.259, DMF).
215 (119 mg. 75%) was synthesized according to procedure A and purified by reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.19 (m, 1H), 0.25 - 0.33 (m, 1H), 0.34 - 0.43 (m, 1H), 0.52 - 0.60 (m, 1H), 1.45 - 1.54 (m, 1H), 1.56 (s, 3H), 1.63 (s, 3H), 2.40 (s, 3H), 3.85 (dd, J=13.4, 5.5 Hz, 1H), 3.89 - 3.94 (m, 1H), 3.95 (s, 3H), 5.52 (s, 1H), 5.57 (s, 1H), 7.22 - 7.30 (m, 2H), 7.43 (d, J=1.3 Hz, 1H), 7.64 - 7.73 (m, 2H), 7.82 (d, J=1.5 Hz, 1H), 8.14 (s, 1H), 8.29 (d, J=10.1 Hz, 1H), 8.59 (t, J=5.9 Hz, 1H); LC-MS (method F): Rt = 2.53 min; mass calcd. for C31H30ClF2N3O4 581.0, m/z found 582.4 [M+H]+; [α]D20 -7.84° (c 0.268, DMF).
216 (110 mg. 68%) was synthesized according to procedure A then purified via reverse phase HPLC (Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.20 (m, 1H), 0.25 - 0.34 (m, 1H), 0.34 - 0.46 (m, 1H), 0.53 - 0.61 (m, 1H), 1.47 (s, 3H), 1.51 - 1.61 (m, 4H), 2.40 (s, 3 H), 3.85 - 3.99 (m, 5H), 5.55 (s, 1H), 5.56 (s, 1H), 7.27 - 7.36 (m, 2H), 7.43 (d, J=1.5 Hz, 1H), 7.84 (d, J=1.5 Hz, 1H), 7.94 (d, J=5.7 Hz, 1H), 7.98 (dd, J=7.5, 5.5 Hz, 2H), 8.27 (d, J=10.3 Hz, 1H), 8.58 (t, J=5.8 Hz, 1H); LC-MS (method F): Rt = 2.53 min; mass calcd. for C31H30F3N3O4 565.0, m/z found 566.4 [M+H]+; [α]D20 -30.36° (c 0.263, DMF).
217 (270 mg. 88%) was synthesized according to procedure B. A purification was performed via SFC (stationary phase: Chiralpak Diacel AD 20 x 250 mm, mobile phase: CO2, EtOH + 0.4% i-PrNH2). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.57 (t, J=5.9 Hz, 1H), 8.30 (d, J=10.2 Hz, 1H), 8.14 (d, J=8.1 Hz, 2H), 8.01 (d, J=5.7 Hz, 1H), 7.81 - 7.87 (m, 3H), 7.43 (d, J=1.2 Hz, 1H), 5.60 (s, 1H), 5.55 (s, 1H), 3.87 - 3.99 (m, 2H), 3.94 (s, 3H), 2.39 (s, 3H), 1.57 - 1.63 (m, 1H), 1.55 (s, 3H), 1.48 (s, 3H), 0.55 - 0.64 (m, 1H), 0.38 - 0.46 (m, 1H), 0.27 - 0.36 (m, 1H), 0.12 - 0.22 (m, 1H); LC-MS (method C): Rt 2.31 min; mass calcd. for C32H30F5N3O4 615.2, m/z found 616.2 [M+H]+; [α]D20 -33.5° (c 0.41, DMF).
218 (192 mg. 66%) was synthesized according to procedure B. A purification was performed via preparatory HPLC (stationary phase: RP XBridge Prep C18 OBD-10µm, 50x150mm, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN).1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.21 (m, 1H), 0.25 - 0.34 (m, 1H), 0.37 - 0.46 (m, 1H), 0.55 - 0.63 (m, 1H), 1.47 (s, 3H), 1.54 (s, 3H), 1.55 - 1.61 (m, 1H), 2.40 (s, 3H), 3.84 - 3.91 (m, 1H), 3.94 (s, 3H), 3.95 - 4.01 (m, 1H), 5.55 (s, 1H), 5.58 (s, 1H), 7.43 (d, J=1.2 Hz, 1H), 7.51 - 7.60 (m, 1H), 7.77 - 7.83 (m, 1H), 7.85 (d, J=1.2 Hz, 1H), 7.94 - 8.02 (m, 2H), 8.29 (dd, J=10.2, 0.8 Hz, 1H), 8.57 (t, J=5.9 Hz, 1H); LC-MS (method A): Rt 9.45 min; mass calcd. for C31H29F4N3O4 583.2, m/z found 584.2 [M+H]+; [α]D20 -36.01° (c 0.281, DMF).
219 (120 mg. 76%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6,) δ ppm 0.72 - 0.81 (m, 2H), 0.81 - 0.90 (m, 2H), 1.58 (s, 3H), 1.65 (s, 3H), 2.48 (s, 3H), 3.99 - 4.05 (m, 1H), 4.11 (dd, J=13.9, 5.3 Hz, 1H), 4.19 - 4.28 (m, 1H), 5.68 (s, 1H), 7.26 -7.33 (m, 2H), 7.43 (s, 1H), 7.68 (d, J=1.5 Hz, 1H), 7.69 - 7.74 (m, 2H), 7.78 (d, J=1.5 Hz, 1H), 8.02 - 8.06 (m, 1H), 8.34 (s, 1H), 8.70 (t, J=5.9 Hz, 1H), 8.76 (d, J=2.2 Hz, 1H); LC-MS (method D): Rt 2.48 min; mass calcd. for C31H28ClF4N3O4 617.0, m/z found 618.0 [M+H]+; [α]D20 -73.66° (c 0.253, DMF).
272 (121 mg, 76%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 um (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 70:30 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.71 - 0.80 (m, 2H), 0.80 - 0.90 (m, 2H), 1.49 (s, 3H), 1.56 (s, 3H), 2.48 (s, 3H), 4.00 (tt, J=6.0, 2.9 Hz, 1H), 4.12 - 4.20 (m, 1H), 4.24 - 4.31 (m, 1H), 5.67 (s, 1H), 7.32 - 7.39 (m, 2H), 7.40 (s, 1H), 7.67 (d, J=1.5 Hz, 1H), 7.81 (d, J=1.5 Hz, 1H), 7.98 - 8.04 (m, 3H), 8.14 (d, J=5.3 Hz, 1H), 8.68 (t, J=5.9 Hz, 1H), 8.75 (d, J=2.2 Hz, 1H); LC-MS (method G): Rt 2.16 min; mass calcd. for C31H28F5N3O4 601.2, m/z found 602.0 [M+H]+; [α]D20 -167° (c 0.3, DMF).
220 (95 mg. 60%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.69 - 0.76 (m, 2H), 0.76 - 0.83 (m, 2H), 1.61 (s, 6H), 2.46 (s, 3H), 3.90 - 3.97 (m, 1H), 4.07 (dd, J=13.8, 5.0 Hz, 1H), 4.41 (dd, J=13.8, 6.5 Hz, 1H), 5.60 (s, 1H), 7.08 (s, 1H), 7.30 - 7.39 (m, 2H), 7.58 (d, J=1.5 Hz, 1H), 7.73 (d, J=1.5 Hz, 1H), 7.93 (dd, J=7.4, 5.6 Hz, 2H), 7.97 (d, J=0.9 Hz, 1H), 8.54 (t, J=5.9 Hz, 1H), 8.73 (d, J=2.0 Hz, 1H); LC-MS (method B): Rt 1.10 min; mass calcd. for C31H27F6N3O4 619.0, m/z found 620.0 [M+H]+; [α]D20 -26.64° (c 0.259, DMF).
221 (110 mg, 71%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: 250 g YMC Tri-Art, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 65:35 to 35:65). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.71 -0.86 (m, 4H), 1.66 (s, 3H), 1.67 (s, 3H), 2.48 (s, 3H), 3.94 - 4.1 (m, 1H), 4.04 (dd, J=13.9, 5.1 Hz, 1H), 4.40 (dd, J=13.8, 6.7 Hz, 1H), 5.53 (s, 1H), 6.95 (s, 1H), 7.23 - 7.31 (m, 2H), 7.53 -7.64 (m, 3H), 7.74 (d, J=1.5 Hz, 1H), 8.02 (d, J=1.1 Hz, 1H), 8.54 (t, J=6.1 Hz, 1H), 8.75 (d, J=2.0 Hz, 1H); LC-MS (method G): Rt 2.14 min; mass calcd. for C31H27ClF5N3O4 635.2, m/z found 636.2 [M+H]+; [α]D20 -46.32° (c 0.272, DMF).
222 (115 mg, 71%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18, 100A, 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.19 (m, 1H), 0.26 - 0.34 (m, 1H), 0.35 - 0.43 (m, 1H), 0.53 - 0.61 (m, 1H), 0.72 - 0.89 (m, 4H), 1.45 - 1.53 (m, 1H), 1.57 (s, 3H), 1.64 (s, 3H), 2.48 (s, 3H), 3.86 (dd, J=13.4, 5.3 Hz, 1H), 3.94 (dd, J=13.4, 6.4 Hz, 1H), 3.99 - 4.05 (m, 1H), 5.55 (s, 1H), 5.57 (s, 1H), 7.23 - 7.30 (m, 2H), 7.66 - 7.73 (m, 3H), 7.74 (d, J=1.8 Hz, 1H), 8.00 (dd, J=1.9, 1.0 Hz, 1H), 8.16 (s, 1H), 8.54 (t, J=5.8 Hz, 1H), 8.75 (d, J=2.2 Hz, 1H); LC-MS (method F): Rt = 2.55 min; mass calcd. for C33H33ClFN3O4 589.0, m/z found 590.4 [M+H]+; [α]D20 -82.15° (c 0.261, DMF).
223 (112 mg. 68%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.11 - 0.20 (m, 1H), 0.26 - 0.34 (m, 1H), 0.36 - 0.46 (m, 1H), 0.54 - 0.63 (m, 1H), 0.70 - 0.80 (m, 2H), 0.80 - 0.88 (m, 2H), 1.48 (s, 3H), 1.51 - 1.60 (m, 4H), 2.47 (s, 3H), 3.85 - 3.92 (m, 1H), 3.93 - 4.04 (m, 2H), 5.55 (br s, 1H), 5.57 (br s, 1H), 7.26 - 7.37 (m, 2H), 7.67 (d, J=1.8 Hz, 1H), 7.77 (d, J=1.5 Hz, 1H), 7.92 - 8.03 (m, 4H), 8.52 (t, J=5.7 Hz, 1H), 8.74 (d, J=2.0 Hz, 1H); LC-MS (method F): Rt = 2.54 min; mass calcd. for C33H33F2N3O4 573.0, m/z found 574.5 [M+H]+; [α]D20 -29.64° (c 0.253, DMF).
224 (301 mg. 97%) was synthesized according to procedure B. 1H NMR (400 MHz, DMSO-d6) δ ppm 0.12 - 0.21 (m, 1H), 0.27 - 0.36 (m, 1H), 0.38 - 0.47 (m, 1H), 0.56 - 0.65 (m, 1H), 0.70 -0.89 (m, 4H), 1.48 (s, 3H), 1.53 - 1.61 (m, 4H), 2.47 (s, 3H), 3.91 (dd, J=13.4, 5.3 Hz, 1H), 3.94 - 4.03 (m, 2H), 5.56 (s, 1H), 5.60 (s, 1H), 7.67 (d, J=1.6 Hz, 1H), 7.79 (d, J=1.2 Hz, 1H), 7.85 (d, J=8.5 Hz, 2H), 8.00 - 8.04 (m, 2H), 8.15 (br d, J=8.1 Hz, 2H), 8.51 (t, J=5.7 Hz, 1H), 8.74 (d, J=2.0 Hz, 1H); LC-MS (method H): Rt = 2.25 min; mass calcd. for C34H33F4N3O4 623.2, m/z found 624.5 [M+H]+; [α]D20 -38.23° (c 0.293, DMF).
225 (290 mg. 98%) was synthesized according to procedure B. 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.21 (m, 1H), 0.26 - 0.34 (m, 1H), 0.38 - 0.46 (m, 1H), 0.56 - 0.64 (m, 1H), 0.70 -0.80 (m, 2H), 0.81 - 0.88 (m, 2H), 1.47 (s, 3H), 1.52 - 1.60 (m, 4H), 2.47 (s, 3H), 3.87 (dd, J=13.4, 5.3 Hz, 1H), 3.96 - 4.04 (m, 2H), 5.56 (s, 1H), 5.58 (s, 1H), 7.55 (dt, J=10.6, 8.5 Hz, 1H), 7.66 (d, J=1.6 Hz, 1H), 7.77 (d, J=2.0 Hz, 1H), 7.78 - 7.84 (m, 1H), 7.94 - 8.04 (m, 3H), 8.50 (t, J=5.9 Hz, 1H), 8.74 (d, J=2.0 Hz, 1H); LC-MS (method H): Rt = 2.15 min; mass calcd. for C33H32F3N3O4 591.2, m/z found 592.5 [M+H]+; [α]D20 -32.28° (c 0.361, DMF).
226 (126 mg. 77%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 9.06 (d, J=2.0 Hz, 1H), 8.62 (t, J=5.9 Hz, 1H), 8.58 (d, J=1.8 Hz, 1H), 8.15 (s, 1H), 7.98 (d, J=1.5 Hz, 1H), 7.66 - 7.71 (m, 2H), 7.52 (d, J=1.3 Hz, 1H), 7.34 (t, J=55., 1H), 7.21 - 7.28 (m, 2H), 5.58 (s, 1H), 5.51 (s, 1H), 4.00 (s, 3H), 3.90 - 3.96 (m, 1H), 3.82 - 3.89 (m, 1H), 1.63 (s, 3H), 1.56 (s, 3H), 1.47 - 1.54 (m, 1H), 0.53 - 0.62 (m, 1H), 0.35 - 0.43 (m, 1H), 0.26 - 0.34 (m, 1H), 0.11 - 0.19 (m, 1H); LC-MS (method B): Rt = 1.09 min; mass calcd. for C31H29ClF3N3O4 599.0, m/z found 600.0 [M+H]+; [α]D20 -9.94° (c 0.251, DMF).
227 (255 mg. 60%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: 250 g, YMC Tri-Art, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 65:35 to 35:65). 1H NMR (400 MHz, DMSO-d6) δ ppm 9.05 (d, J=2.0 Hz, 1H), 8.60 (t, J=5.9 Hz, 1H), 8.56 (d, J=1.8 Hz, 1H), 7.93 - 8.02 (m, 4H), 7.52 (d, J=1.5 Hz, 1H), 7.33 (t, J=55.1 Hz, 1H), 7.27 - 7.34 (m, 2H), 5.56 (s, 1H), 5.51 (s, 1H), 3.99 (s, 3H), 3.87 - 3.98 (m, 2H), 1.55 - 1.61 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 0.55 - 0.63 (m, 1H), 0.37 - 0.46 (m, 1H), 0.27 - 0.35 (m, 1H), 0.12 - 0.20 (m, 1H); LC-MS (method D): Rt = 2.44 min; mass calcd. for C31H29F4N3O4 583.0, m/z found 584.0 [M+H]+; [α]D20 -32.4° (c 0.25, DMF).
228 (61 mg. 49%) was synthesized according to procedure D. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 98:2). The residue was diluted in EtOH and evaporated under reduced pressure (3 times). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.73 (d, J=2.1 Hz, 1H), 8.54 (br t, J=5.9 Hz, 1H), 7.93 (br dd, J=7.2, 5.9 Hz, 2H), 7.82 (d, J=1.8 Hz, 1H), 7.69 (d, J=1.0 Hz, 1H), 7.36 (t, J=8.8 Hz, 2H), 7.23 (s, 1H), 7.05 (s, 1H), 5.63 (s, 1H), 4.40 (br dd, J=13.7, 6.7 Hz, 1H), 4.07 (br dd, J=14.2, 4.8 Hz, 1H), 3.90 (s, 3H), 2.09 -2.18 (m, 1H), 1.60 (s, 6H), 1.06 - 1.11 (m, 2H), 0.81 - 0.88 (m, 2H); LC-MS (method I): Rt = 3.05 min; mass calcd. for C31H27F6N3O4 619.2, m/z found 620.2 [M+H]+; [α]D20 -22.31° (c 0.26, DMF).
229 (73 mg. 46%) was synthesized according to procedure A and was purified via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 85:15 to 25:75). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.51 (s, 3H), 1.57 (s, 3H), 1.63 (s, 3H), 2.88 (s, 3H), 3.64 - 3.78 (m, 2H), 4.08 (s, 3H), 5.58 (s, 1H), 5.71 (s, 1H), 7.20 - 7.28 (m, 2H), 7.43 (d, J=1.3 Hz, 1H), 7.60 - 7.69 (m, 2H), 7.79 (d, J=1.5 Hz, 1H), 7.91 (s, 1H), 8.24 (s, 1H), 8.60 (t, J=6.1 Hz, 1H); LC-MS (method B): Rt 0.91 min; mass calcd. for C28H28ClFN4O4 538.0, m/z found 539.3 [M+H]+; [α]D20 -37.31° (c 0.26, DMF).
230 (63 mg. 38%) was synthesized according to procedure A and purified via reverse phase HPLC (stationary phase: 250 g YMC Tri-Art, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 65:35 to 35:65).1H NMR (400 MHz, DMSO-d6) δ ppm 1.51 (s, 3H), 1.57 (s, 3H), 1.63 (s, 3H), 2.88 (s, 3H), 3.63 - 3.77 (m, 2H), 4.08 (s, 3H), 5.58 (s, 1H), 5.72 (s, 1H), 7.20 - 7.28 (m, 2H), 7.43 (d, J=1.3 Hz, 1H), 7.61 - 7.68 (m, 2H), 7.79 (d, J=1.5 Hz, 1H), 7.91 (s, 1H), 8.24 (s, 1H), 8.60 (t, J=6.2 Hz, 1H); LC-MS (method D): Rt 2.31 min; mass calcd. for C28H28ClFN4O4 538.0, m/z found 539 [M+H]+; [α]D20 +36.01° (c 0.256, DMF).
231 (398 mg. 79%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 85:15 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.40 (dd, J=6.4, 4.8 Hz, 1H), 7.92 - 8.00 (m, 3H), 7.79 (s, 1H), 7.63 (d, J=1.3 Hz, 1H), 7.26 - 7.33 (m, 3H), 5.56 (s, 1H), 5.54 (s, 1H), 3.96 - 4.09 (m, 1H), 4.02 (s, 3H), 3.73 (dd, J=13.3, 4.5 Hz, 1H), 2.86 (s, 3H), 2.45 (quin, J=6.9 Hz, 1H), 1.53 (s, 3H), 1.44 (s, 3H), 1.02 (d, J=6.6 Hz, 3H), 0.72 (d, J=6.8 Hz, 3H); LC-MS (method G): Rt 1.93 min; mass calcd. for C30H32F2N4O4 536.2, m/z found 537.2 [M+H]+; [α]D20 -82.1° (c 0.525, DMF).
232 (122 mg. 81%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 70:30 to 10:90).1H NMR (400 MHz, DMSO-d6) δ ppm 1.57 (s, 3H), 1.65 (s, 3H), 2.87 (s, 3H), 4.05 - 4.15 (m, 4H), 4.18 - 4.26 (m, 1H), 5.69 (s, 1H), 7.25 - 7.34 (m, 3H), 7.39 (d, J=1.3 Hz, 1H), 7.65 - 7.73 (m, 2H), 7.76 (d, J=1.5 Hz, 1H), 7.95 (s, 1H), 8.33 (s, 1H), 8.80 (t, J=5.7 Hz, 1H); LC-MS (method B): Rt 1.10 min; mass calcd. for C28H25ClF4N4O4 592.0, m/z found 593.0 [M+H]+; [α]D20 -67.53° (c 0.270, DMF).
233 (54 mg. 47%) was synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1). A second purification was performed via reverse phase (stationary phase: YMC-actus Triart C18 10 µm 30 x 150 mm, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 65:35 to 45:55). The residue was co-evaporated with EtOH (3 times) and dried under vacuum at 50° C. for 5 h. 1H NMR (500 MHz, DMSO-d6, 30° C.) δ ppm 1.75 (s, 3H), 1.78 (s, 3H), 2.84 (s, 3H), 2.87 (s, 3H), 4.04 - 4.11 (m, 4H), 4.27 (br dd, J=13.9, 6.3 Hz, 1H), 7.26 - 7.34 (m, 3H), 7.39 (s, 1H), 7.69 (dd, J=8.5, 5.7 Hz, 2H), 7.74 (s, 2H), 7.95 (d, J=3.8 Hz, 2H), 8.74 (t, J=5.5 Hz, 1H); LC-MS (method I): Rt = 2.69 min; mass calcd. for C29H28ClF4N5O5S 669.1, m/z found 670.3 [M+H]+; [α]D20 +64.75° (c 0.278, DMF).
234 (1.7 g. 83%) was synthesized according to procedure B. The reaction was quenched by the addition of water and the mixture was stirred for few hours. The supernatant was removed. The resulting solid was triturated in water and collected by filtration. The residue was purified by silica column chromatography (EtOAc). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.49 (s, 3H), 1.56 (s, 3H), 2.87 (s, 3H), 4.07 (s, 3H), 4.16 (dd, J=14.0, 5.6 Hz, 1H), 4.26 (dd, J=14.0, 6.2 Hz, 1H), 5.67 (s, 1H), 7.29 (s, 1H), 7.32 - 7.38 (m, 2H), 7.39 (d, J=1.3 Hz, 1H), 7.78 (d, J=1.5 Hz, 1H), 7.93 (s, 1H), 8.00 (dd, J=7.6, 5.6 Hz, 2H), 8.13 (d, J=5.5 Hz, 1H), 8.78 (t, J=5.9 Hz, 1H); LC-MS (method B): Rt = 1.01 min; mass calcd. for C28H25F5N4O4 576.0, m/z found 577.3 [M+H]+; [α]D20 -46.67° (c 0.09 DMF).
235 (38 mg. 26%) and 236 (35 mg, 24%) were synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 96:4). The residue was diluted with EtOH and evaporated under reduced pressure (twice). A second purification was performed by chiral SFC (stationary phase: CHIRALPAK AD-H 5 µm 250 x 30 mm, mobile phase: 80% CO2, 20% EtOH) to give 235 (52 mg); 1H NMR (500 MHz, DMSO-d6, 31° C.) δ ppm 1.61 (s, 3H), 1.62 (s, 3H), 1.83 (s, 3H), 2.86 (s, 3H), 4.06 (s, 3H), 4.10 (dd, J=13.9, 5.4 Hz, 1H), 4.29 (br dd, J=13.9, 6.6 Hz, 1H), 7.24 (s, 1H), 7.31 - 7.37 (m, 2H), 7.39 (d, J=1.3 Hz, 1H), 7.71 - 7.77 (m, 2H), 7.97 (br dd, J=8.0, 5.8 Hz, 2H), 7.99 (s, 1H), 8.35 (s, 1H), 8.71 (br t, J=5.8 Hz, 1H); LC-MS (method I): Rt = 2.68 min; mass calcd. for C30H28F5N5O4 617.2, m/z found 618.4 [M+H]+; [α]D20 -74.23° (c 0.26, DMF); and 236 (51 mg); 1H NMR (500 MHz, DMSO-d6, 31° C.) δ ppm 1.61 (br s, 3H), 1.62 (br s, 3H), 1.83 (s, 3H), 2.86 (s, 3H), 4.06 (s, 3H), 4.10 (dd, J=14.1, 5.5 Hz, 1H), 4.29 (dd, J=14.1, 6.3 Hz, 1H), 7.25 (br s, 1H), 7.31 - 7.37 (m, 2H), 7.39 (d, J=1.3 Hz, 1H), 7.72 - 7.76 (m, 2H), 7.97 (dd, J=8.0, 5.8 Hz, 3H), 7.99 (s, 1H), 8.35 (s, 1H), 8.72 (br t, J=5.7 Hz, 1H); LC-MS (method I): Rt = 2.68 min; mass calcd. for C30H28F5N5O4 617.2, m/z found 618.4 [M+H]+; [α]D20 +87.31° (c 0.26, DMF).
237 (80 mg. 64%) was synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 98:2). The crude was diluted with EtOH and evaporated under reduced pressure (twice). A second purification was performed by reverse phase (spherical C18, 25 µm, 40 g YMC-ODS-25, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 50:50 to 10:90). The residue was diluted with EtOH and evaporated under reduced pressure (twice). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.70 (s, 6H), 2.86 (s, 3H), 2.87 (s, 3H), 4.07 (s, 3H), 4.14 (br dd, J=13.8, 4.4 Hz, 1H), 4.25 - 4.33 (m, 1H), 7.31 - 7.42 (m, 4H), 7.77 (s, 1H), 7.85 (br s, 1H), 7.92 - 7.96 (m, 2H), 8.00 (br dd, J=7.6, 6.1 Hz, 2H), 8.78 (br t, J=5.7 Hz, 1H); LC-MS (method I): Rt = 2.71 min; mass calcd. for C29H28F5N5O5S 653.2, m/z found 654.3 [M+H]+; [α]D20 -78.89° (c 0.27, DMF).
238 (93 mg. 51%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.49 (s, 3H), 1.55 (s, 3H), 2.26 (s, 3H), 2.86 (s, 3H), 4.06 (s, 3H), 4.13 (br dd, J=14.0, 5.4 Hz, 1H), 4.28 (br dd, J=14.0, 6.1 Hz, 1H), 5.66 (s, 1H), 7.22 - 7.43 (m, 3H), 7.74 - 7.86 (m, 3H), 7.91 (s, 1H), 8.11 (d, J=5.3 Hz, 1H), 8.80 (br t, J=5.7 Hz, 1H); LC-MS (method G): Rt 2.04 min; mass calcd. for C29H27F5N4O4 590.2, m/z found 591.2 [M+H]+; [α]D20 -79.08° (c 0.502, DMF).
239 (71 mg. 47%) was synthesized according to procedure B. The reaction was quenched by the addition of water. The mixture was stirred for few hours and the surnatant was removed. The solid was triturated in water and collected by filtration. The solid was purified via SFC (Chiralpak Daicel IC 20 x 250 mm, mobile phase: CO2, EtOH + 0.4% i-PrNH2). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.47 (s, 3H), 1.55 (s, 3H), 2.86 (s, 3H), 4.06 (s, 3H), 4.11 (dd, J=13.9, 5.5 Hz, 1H), 4.31 (dd, J=13.8, 6.3 Hz, 1H), 5.68 (s, 1H), 7.31 (s, 1H), 7.38 (d, J=1.3 Hz 1H), 7.58 (dt, J=10.6, 8.6 Hz, 1H), 7.78 (d, J=1.3 Hz, 1H), 7.79 - 7.85 (m, 1H), 7.95 (s, 1H), 7.98 (ddd, J=11.9, 8.1, 1.8 Hz, 1H), 8.15 (d, J=5.3 Hz, 1H), 8.77 (t, J=5.9 Hz, 1H); LC-MS (method H): Rt = 1.99 min; mass calcd. for C28H24F6N4O4 594.2, m/z found 594.4 [M+H]+; [α]D20 -97.85° (c 0.255, DMF).
240 (220 mg. 58%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (Kromasil C18, 100A, 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 90:10 to 30:70). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.62 (s, 6H), 2.85 (s, 3H), 4.02 (s, 3H), 4.09 (dd, J=14.0, 5.2 Hz, 1H), 4.39 (dd, J=13.8, 6.7 Hz, 1H), 5.60 (s, 1H), 7.00 (s, 1H), 7.31 (d, J=1.3 Hz, 1H), 7.32 - 7.38 (m, 2H), 7.71 (d, J=1.5 Hz, 1H), 7.89 (s, 1H), 7.92 (dd, J=7.4, 5.6 Hz, 2H), 8.69 (t, J=6.1 Hz, 1H); LC-MS (method D): Rt 2.31 min; mass calcd. for C28H24F6N4O4 594.0, m/z found 595.0 [M+H]+; [α]D20 -25.19° (c 0.27, DMF).
241 (78 mg, 54%) was synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1). The crude was co-evaporated with EtOH (3 times) and dried under vacuum at 60° C. for 18 h. A second purification was performed by reverse phase (stationary phase: YMC-actus Triart C18 10 µm 30 x150 mm, 40 g, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 75:25 to 35:65). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.67 (br s, 3H), 1.69 (br s, 3H), 1.76 (s, 3H), 2.85 (s, 3H), 4.02 (s, 3H), 4.03 - 4.09 (m, 1H), 4.44 (br dd, J=13.9, 6.7 Hz, 1H), 7.00 (s, 1H), 7.29 - 7.39 (m, 3H), 7.72 (d, J=1.1 Hz, 1H), 7.87 - 7.95 (m, 3H), 8.57 (s, 1H), 8.66 (br t, J=5.9 Hz, 1H); LC-MS (method I): Rt = 2.72 min; mass calcd. for C30H27F6N5O4 635.2, m/z found 636.5 [M+H]+; [α]D20 = -27.18° (c 0.287, DMF).
242 (81 mg. 55%) was synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1). The residue was co-evaporated with EtOH (3 times) and dried under vacuum at 50° C. for 5 h. A second purification was performed by reverse phase (stationary phase: YMC-actus Triart C18 10 µm 30 x 150 mm, 40 g, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 75:25 to 35:65). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.67 (br s, 3H), 1.69 (br s, 3H), 1.76 (s, 3H), 2.85 (s, 3H), 4.02 (s, 3H), 4.03 - 4.09 (m, 1H), 4.44 (br dd, J=13.6, 6.9 Hz, 1H), 7.00 (s, 1H), 7.31 (d, J=0.6 Hz, 1H), 7.32 - 7.39 (m, 2H), 7.72 (d, J=0.7 Hz, 1H), 7.87 - 7.96 (m, 3H), 8.57 (s, 1H), 8.66 (br t, J=5.9 Hz, 1H); LC-MS (method I): Rt = 2.72 min; mass calcd. for C30H27F6N5O4 635.2, m/z found 636.5 [M+H]+; [α]D20 +22° (c 0.300, DMF).
243 (78 mg, 55%) was synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1). The residue was co-evaporated with EtOH (3 times) and dried under vacuum at 50° C. for 5 h. A second purification was performed by reverse phase (stationary phase: YMC-actus Triart C18 10 µm 30 x150 mm, 40 g, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 75:25 to 35:65). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.78 (br s, 6H), 2.85 (s, 3H), 2.87 (s, 3H), 4.02 (s, 3H), 4.12 (br dd, J=13.8, 4.5 Hz, 1H), 4.39 (dd, J=13.3, 6.0 Hz, 1H), 7.06 (br s, 1H), 7.32 (d, J=1.0 Hz, 1H), 7.33 - 7.40 (m, 2H), 7.71 (d, J=1.1 Hz, 1H), 7.87 - 8.01 (m, 4H), 8.70 (t, J=5.8 Hz, 1H); LC-MS (method I): Rt = 2.73 min; mass calcd. for C29H27F6N5O5S 671.2, m/z found 672.5 [M+H]+; [α]D20 -21.72° (c 0.29, DMF).
244 (74 mg. 52%) was synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1). The residue was co-evaporated with EtOH (3 times) and dried under vacuum at 50° C. for 5 h. A second purification was performed by reverse phase (stationary phase: YMC-actus Triart C18 10 µm 30 x 150 mm, 40 g, mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 75:25 to 35:65). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.78 (br s, 6H), 2.85 (s, 3H), 2.87 (s, 3H), 4.02 (s, 3H), 4.08 - 4.16 (m, 1H), 4.39 (br dd, J=14.2, 5.4 Hz, 1H), 6.99 - 7.14 (m, 1H), 7.32 (d, J=1.0 Hz, 1H), 7.34 - 7.40 (m, 2H), 7.72 (d, J=1.0 Hz, 1H), 7.87 - 8.01 (m, 4H), 8.64 - 8.75 (m, 1H); LC-MS (method I): Rt = 2.73 min; mass calcd. for C29H27F6N5O5S 671.2, m/z found 672.5 [M+H]+; [α]D20 +14.8° (c 0.25, DMF).
The racemic product was synthesized according to procedure A and purified via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 70:30 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.51 (t, J=5.9 Hz, 1H), 7.85 (dd, J=7.5, 5.7 Hz, 2H), 7.75 (s, 1H), 7.64 (d, J=1.3 Hz, 1H), 7.24 -7.31 (m, 3H), 5.91 (s, 1H), 5.32 (s, 1H), 4.12 (dd, J=13.2, 6.6 Hz, 1H), 3.99 (s, 3H), 3.83 (dd, J=13.4, 5.3 Hz, 1H), 2.91 (s, 3H), 2.84 (s, 3H), 1.67 - 1.75 (m, 1H), 1.65 (d, J=4.4 Hz, 3H), 1.60 (d, J=2.6 Hz, 3H), 0.59 - 0.67 (m, 1H), 0.37 - 0.47 (m, 1H), 0.19 - 0.34 (m, 2H); LCMS (method B): Rt 1.08 min; mass calcd. for C31H32F2N4O4 562.0, m/z found 563.0 [M+H]+. Then the isomers were separated by SFC (stationary phase: Daicel Chiralpak IC 250 g, 5 µm, mobile phase: heptane/EtOH, 60:40) to afford 245 (88 mg. 18%) and 246 (97 mg, 20%).
247 (108 mg. 88%) was synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1). The residue was co-evaporated with EtOH (3 times) and dried under vacuum at 50° C. for 3 h. 1H NMR (400 MHz, DMSO-d6) δ ppm 1.66 (br s, 6H), 2.86 (s, 3H), 4.00 - 4.10 (m, 4H), 4.37 (br dd, J=13.5, 6.5 Hz, 1H), 5.54 (s, 1H), 6.93 (s, 1H), 7.23 - 7.33 (m, 3H), 7.54 - 7.61 (m, 2H), 7.72 (d, J=1.0 Hz, 1H), 7.94 (s, 1H), 8.72 (t, J=6.1 Hz, 1H); LC-MS (method I): Rt = 2.83 min; mass calcd. for C28H24ClF5N4O4 610.1, m/z found 611.3 [M+H]+; [α]D20 -44.56° (c 0.285, DMF).
248 (112 mg. 91%) was synthesized according to procedure C. The crude mixture was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 100:0 to 99:1). The residue was co-evaporated with EtOH (3 times) and dried under vacuum at 50° C. for 3 h. 1H NMR (400 MHz, DMSO-d6) δ ppm 1.66 (br s, 6H), 2.86 (s, 3H), 4.01 - 4.09 (m, 4H), 4.32 - 4.42 (m, 1H), 5.54 (s, 1H), 6.93 (s, 1H), 7.24 - 7.33 (m, 3H), 7.53 - 7.62 (m, 2H), 7.72 (d, J=1.0 Hz, 1H), 7.94 (s, 1H), 8.72 (t, J=6.0 Hz, 1H); LC-MS (method I): Rt = 2.83 min; mass calcd. for C28H24ClF5N4O4 610.1, m/z found 611.3 [M+H]+; [α]D20 +45.69° (c 0.267, DMF).
249 (74 mg. 32%) and 250 (78 mg, 33%) were synthesized according to procedure A. A purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80) to afford a racemic mixture. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.69 (t, J=6.1 Hz, 1H), 8.04 (d, J=5.5 Hz, 1H), 7.93 (dd, J=7.6, 5.6 Hz, 2H), 7.82 (s, 1H), 7.71 (d, J=1.3 Hz, 1H), 7.34 (d, J=1.3 Hz, 1H), 7.19 - 7.30 (m, 2H), 6.66 (t, J=54.8 Hz, 1H), 6.56 (s, 1H), 5.62 (s, 1H), 3.96 - 4.11 (m, 4H), 3.78 - 3.86 (m, 1H), 2.86 (s, 3H), 1.54 (s, 3H), 1.45 (s, 3H); LC-MS (method B): Rt 0.93 min; mass calcd. for C28H26F4N4O4 558.0, m/z found 559.0 [M+H]+. The enantiomers were separated by SFC (stationary phase: Daicel Chiralpak IC 250 g, 5 µm, mobile phase: heptane/EtOH 86:14) afforded 249; [α]D20 +82.53 (c 0.261, DMF); and 250; [α]D20 -73.76° (c 0.263, DMF).
251 (292 mg. 63%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 85:15 to 25:75). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.21 (m, 1H), 0.27 - 0.35 (m, 1H), 0.35 - 0.43 (m, 1H), 0.53 - 0.62 (m, 1H), 1.47 - 1.54 (m, 1H), 1.56 (s, 3H), 1.64 (s, 3H), 2.87 (s, 3 H), 3.81 - 3.96 (m, 2H), 4.07 (s, 3H), 5.44 (s, 1H), 5.57 (s, 1H), 7.21 - 7.30 (m, 2H), 7.39 (d, J=1.3 Hz, 1H), 7.65 - 7.71 (m, 2H), 7.74 (d, J=1.3 Hz, 1H), 7.91 (s, 1H), 8.15 (s, 1H), 8.66 (t, J=5.8 Hz, 1H); LC-MS (method B): Rt 1.00 min; mass calcd. for C30H30ClFN4O4 564.0, m/z found 565.4 [M+H]+; [α]D20 -5.13° (c 0.526, DMF).
252 (288 mg. 73%) was synthesized according to procedure B. The reaction was quenched by the addition of water and the mixture was stirred for few hours. The surnatant was removed. The resulting solid was triturated in water and filtered off. The residue was purified by silica column chromatography (EtOAc). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.12 - 0.21 (m, 1H), 0.28 -0.36 (m, 1H), 0.37 - 0.46 (m, 1H), 0.55 - 0.63 (m, 1H), 1.48 (s, 3H), 1.54 (s, 3H), 1.56 - 1.62 (m, 1H), 2.86 (s, 3H), 3.86 - 3.98 (m, 2H), 4.06 (s, 3H), 5.43 (s, 1H), 5.56 (s, 1H), 7.27 - 7.35 (m, 2H), 7.40 (d, J=1.3 Hz, 1H), 7.76 (d, J=1.3 Hz, 1H), 7.89 (s, 1H), 7.92 - 8.01 (m, 3H), 8.64 (t, J=5.9 Hz, 1H); LC-MS (method B): Rt = 1.00 min; mass calcd. for C30H30F2N4O4 548.0, m/z found 549.3 [M+H]+; [α]D20 -30.92° (c 0.255, DMF).
253 (199 mg. 67%) was synthesized according to procedure B. The crude was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 99:1 to 95:5). The residue was triturated in DIPE. The solids were collected by filtration and dried under vacuum. 1H NMR (400 MHz, DMSO-d6) δ ppm 0.13 - 0.22 (m, 1H), 0.28 - 0.37 (m, 1H), 0.38 - 0.48 (m, 1H), 0.56 - 0.64 (m, 1H), 1.48 (s, 3H), 1.55 (s, 3H), 1.58 - 1.65 (m, 1H), 2.86 (s, 3H), 3.87 - 3.98 (m, 2H), 4.06 (s, 3H), 5.45 (s, 1H), 5.61 (s, 1H), 7.39 (d, J=1.6 Hz, 1H), 7.77 (d, J=1.6 Hz, 1H), 7.84 (d, J=8.5 Hz, 2H), 7.92 (s, 1H), 8.01 (d, J=5.7 Hz, 1H), 8.14 (d, J=8.1 Hz, 2H), 8.65 (t, J=5.9 Hz, 1H); LC-MS (method H): Rt = 2.08 min; mass calcd. for C31H30F4N4O4 598.2, m/z found 599.4 [M+H]+; [α]D20 -37.77° (c 0.349, DMF).
254 (129 mg. 43%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 60:40 to 20:80). A second purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 50:50 to 10:90). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.66 (t, J=5.8 Hz, 1H), 7.90 (d, J=5.7 Hz, 1H), 7.87 (s, 1H), 7.79 (dd, J=8.1, 1.3 Hz, 2H), 7.76 (d, J=1.3 Hz, 1H), 7.39 (d, J=1.3 Hz, 1H), 7.12 - 7.17 (m, 2H), 5.54 (s, 1H), 5.42 (s, 1H), 4.05 (s, 3H), 3.84 - 3.96 (m, 2H), 2.87 (s, 3H), 1.91 - 2.00 (m, 1H), 1.55 - 1.61 (m, 1H), 1.54 (s, 3H), 1.48 (s, 3H), 0.96 - 1.03 (m, 2H), 0.66 - 0.71 (m, 2H), 0.54 -0.61 (m, 1H), 0.36 - 0.45 (m, 1H), 0.27 - 0.35 (m, 1H), 0.12 - 0.21 (m, 1H); LCMS (method B): Rt 1.13 min; mass calcd. for C33H35FN4O4 570.0, m/z found 571 [M+H]+; [α]D20 -34.45° (c 0.508, DMF).
255 (75 mg. 25%) was synthesized according to procedure A then purified via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 60:40 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.66 (t, J=5.8 Hz, 1H), 7.98 (d, J=5.7 Hz, 1H), 7.91 (s, 1H), 7.69 - 7.80 (m, 3H), 7.48 - 7.56 (m, 1H), 7.39 (d, J=1.3 Hz, 1H), 7.29 (td, J=8.6, 2.0 Hz, 1H), 5.64 (s, 1H), 5.51 (s, 1H), 4.05 (s, 3H), 3.94 - 4.01 (m, 1H), 3.84 - 3.91 (m, 1H), 2.86 (s, 3H), 1.55 - 1.63 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 0.54 - 0.64 (m, 1H), 0.37 - 0.48 (m, 1H), 0.28 - 0.36 (m, 1H), 0.12 - 0.23 (m, 1 H); LC-MS (method B): Rt 1.03 min; mass calcd. for C30H30F2N4O4 548.0, m/z found 549.0 [M+H]+; [α]D20 -34.36° (c 0.521, DMF).
256 (163 mg. 58%) was synthesized according to procedure B. The crude was purified by silica column chromatography (CH2Cl2/CH3OH, gradient from 99:1 to 95:5). The residue was crystallized from DIPE and CH3CN (1:1) and collected by filtration and dried under vacuum. 1H NMR (400 MHz, DMSO-d6) δ ppm 0.12 - 0.22 (m, 1H), 0.27 - 0.35 (m, 1H), 0.38 - 0.46 (m, 1H), 0.56 - 0.64 (m, 1H), 1.47 (s, 3H), 1.54 (s, 3H), 1.55 - 1.62 (m, 1H), 2.86 (s, 3H), 3.88 (dd, J=13.4, 5.7 Hz, 1H), 3.98 (dd, J=13.4, 6.5 Hz, 1H), 4.06 (s, 3H), 5.45 (s, 1H), 5.58 (s, 1H), 7.39 (d, J=1.2 Hz, 1H), 7.54 (dt, J=10.6, 8.5 Hz, 1H), 7.76 (d, J=1.2 Hz, 1H), 7.77 - 7.83 (m, 1H), 7.92 (s, 1H), 7.94 - 8.01 (m, 2H), 8.63 (t, J=5.9 Hz, 1H); LC-MS (method H): Rt = 1.91 min; mass calcd. for C30H29F3N4O4 566.2, m/z found 567.4 [M+H]+; [α]D20 -40.47° (c 0.551, DMF).
257 (50 mg. 65%) was synthesized according to procedure B. A purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 70:30 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.55 (dd, J=6.9, 5.2 Hz, 1H), 7.81 - 7.87 (m, 2H), 7.78 (s, 1H), 7.62 (d, J=1.5 Hz, 1H), 7.25 - 7.32 (m, 2H), 7.24 (d, J=1.5 Hz, 1H), 5.57 (s, 1H), 5.32 (s, 1H), 4.20 (dd, J=13.3, 7.4 Hz, 1H), 3.97 (s, 3H), 3.63 (dd, J=13.5, 4.7 Hz, 1H), 2.84 (s, 3H), 1.65 - 1.73 (m, 1H), 1.63 (s, 6H), 0.65 - 0.74 (m, 1H), 0.41 - 0.50 (m, 1H), 0.20 - 0.34 (m, 2H), LC-MS (method B): Rt = 1.03 min; mass calcd. for C30H29F3N4O4 566.0, m/z found 567.0 [M+H]+.
258 (60 mg. 78%) was synthesized according to procedure B. A purification was performed via reverse phase HPLC (stationary phase: Kromasil, C18, 100A, 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 50:50 to 10:90). 1H NMR (400 MHz, DMSO-d6) δ 8.55 (t, J=1.0 Hz, 1H), 7.84 (dd, J=7.5, 5.5 Hz, 2H), 7.78 (s, 1H), 7.62 (d, J=1.3 Hz, 1H), 7.25 - 7.33 (m, 2H), 7.24 (d, J=1.1 Hz, 1H), 5.57 (br s, 1H), 5.32 (s, 1H), 4.15 - 4.25 (m, 1H), 3.97 (s, 3H), 3.54 - 3.68 (m, 1H), 2.83 (s, 3H), 1.66 - 1.73 (m, 1H), 1.63 (s, 6H), 0.66 - 0.77 (m, 1H), 0.40 - 0.50 (m, 1H), 0.18 - 0.34 (m, 2H); LC-MS (method B): Rt = 1.02 min; mass calcd. for C30H29F3N4O4 566.0, m/z found 567.0 [M+H]+; [α]D20 -14.2° (c 0.65, DMF).
259 (117 mg. 50%) was synthesized according to procedure A and purified by reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 60:40 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.79 (t, J=5.9 Hz, 1H), 8.16 - 8.25 (m, 2H), 7.97 (d, J=1.3 Hz, 1H), 7.91 (s, 1H), 7.88 (d, J=1.1 Hz, 1H), 7.77 (d, J=1.3 Hz, 1H), 7.37 (d, J=1.3 Hz, 1H), 7.30 - 7.36 (m, 2H), 7.22 (s, 1H), 5.37 (s, 1H), 4.32 (dd, J=14.0, 6.5 Hz, 1H), 4.14 (dd, J=13.9, 5.3 Hz, 1H), 4.03 (s, 3H), 2.85 (s, 3H), 1.45 (s, 6H); LC-MS (method B): Rt = 0.99 min; mass calcd. for C28H26F4N4O4 558.0, m/z found 559.0 [M+H]+; [α]D20 -81.9° (c 0.525, DMF).
260 (99 mg. 59%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 85:15 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.21 (m, 1H), 0.26 - 0.44 (m, 2H), 0.53 - 0.63 (m, 1H), 1.48 (s, 3H), 1.54 (s, 3H), 1.50 - 1.61 (m, 2H), 2.92 (s, 3H), 3.92 (d, J=5.9 Hz, 2H), 5.40 (s, 1H), 5.56 (s, 1H), 7.27 - 7.34 (m, 2H), 7.60 (t, J=73.7 Hz, 1H), 7.79 (s, 1H), 7.97 (br dd, J=7.5, 5.7 Hz, 2H), 8.05 (s, 1H), 8.17 (d, J=1.5 Hz, 1H), 8.73 (t, J=6.1 Hz, 1H); LC-MS (method B): Rt 1.07 min; mass calcd. for C30H28F4N4O4 584.0, m/z found 585.3 [M+H]+; [α]D20 -25.75° (c 0.268, DMF).
261 (111 mg. 67%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 1.57 (s, 3H), 1.64 (s, 3H), 2.95 (s, 3H), 4.07 - 4.25 (m, 2H), 5.67 (s, 1H), 7.22 - 7.32 (m, 3H), 7.64 - 7.72 (m, 2H), 7.99 (d, J=1.3 Hz, 1H), 8.18 (s, 1H), 8.32 (s, 1H), 8.36 (d, J=1.5 Hz, 1H), 8.92 (t, J=5.6 Hz, 1H); LC-MS (method B): Rt 1.17 min; mass calcd. for C28H22ClF7N4O4 646.0, m/z found 647.0 [M+H]+; [α]D20 -25.75° (c 0.268, DMF).
262 (96 mg, 61%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.77 - 0.83 (m, 2H), 0.83 - 0.89 (m, 2H), 1.62 (s, 6H), 2.84 (s, 3H), 4.02 - 4.13 (m, 2H), 4.40 (br dd, J=13.8, 6.1 Hz, 1H), 5.61 (s, 1H), 7.04 (s, 1H), 7.31 - 7.38 (m, 2H), 7.61 (d, J=1.5 Hz, 1H), 7.74 (d, J=1.5 Hz, 1H), 7.87 (s, 1H), 7.92 (dd, J=7.5, 5.5 Hz, 2H), 8.70 (br t, J=5.7 Hz, 1H); LC-MS (method B): Rt 1.04 min; mass calcd. for C30H26F6N4O4 620.2, m/z found 621.3 [M+H]+; [α]D20 -27.61° (c 0.268, DMF).
263 (123 mg, 79%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/ CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.78 - 0.93 (m, 4H), 1.67 (br s, 6H), 2.86 (s, 3H), 4.01 - 4.15 (m, 2H), 4.38 (br dd, J=13.8, 6.3 Hz, 1H), 5.53 (s, 1H), 6.93 (s, 1H), 7.22 - 7.31 (m, 2H), 7.54 - 7.61 (m, 2H), 7.63 (d, J=1.5 Hz, 1H), 7.74 (d, J=1.5 Hz, 1H), 7.92 (s, 1H), 8.70 (br t, J=5.8 Hz, 1H); LC-MS (method G): Rt 2.04 min; mass calcd. for C30H26ClF5N4O4 636.2, m/z found 637.2 [M+H]+; [α]D20 -50.1° (c 0.256, DMF).
266 (101 mg. 62%) was synthesized according to procedure A. A purification was performed via reverse phase HPLC (stationary phase: Kromasil C18, 100A, 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.10 - 0.20 (m, 1H), 0.24 - 0.34 (m, 1H), 0.34 - 0.44 (m, 1H), 0.52 - 0.62 (m, 1H), 0.71 - 0.80 (m, 2H), 0.81 - 0.91 (m, 2H), 1.44 - 1.53 (m, 1H), 1.57 (s, 3H), 1.64 (s, 3H), 2.48 (s, 3H), 3.84 (dd, J=13.5, 5.3 Hz, 1H), 3.93 (dd, J=13.4, 6.4 Hz, 1H), 3.98 - 4.05 (m, 1H), 5.55 (s, 1H), 7.20 - 7.32 (m, 2H), 7.65 - 7.73 (m, 3H), 7.74 (d, J=1.5 Hz, 1H), 7.98 - 8.02 (m, 1H), 8.15 (s, 1H), 8.55 (t, J=5.9 Hz, 1H), 8.75 (d, J=2.2 Hz, 1H); LC-MS (method F): Rt = 2.55 min; mass calcd. for C32H32ClFN4O4 590.0, m/z found 590.4 [M]+; [α]D20 -85.6° (c 0.253, DMF).
265 (125 mg. 76%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm -0.06 - 0.05 (m, 1H), 0.09 - 0.18 (m, 1H), 0.19 - 0.30 (m, 1H), 0.37 - 0.47 (m, 1H), 0.61 - 0.78 (m, 4H), 1.30 (s, 3H), 1.36 (s, 3H), 1.38 - 1.44 (m, 1H), 2.68 (s, 3H), 3.68 - 3.83 (m, 2H), 3.91 - 3.98 (m, 1H), 5.25 (s, 1H), 5.38 (s, 1H), 7.08 - 7.18 (m, 2H), 7.53 (d, J=1.5 Hz, 1H), 7.59 (d, J=1.5 Hz, 1H), 7.69 (s, 1H), 7.74 - 7.85 (m, 3H), 8.44 (t, J=5.8 Hz, 1H); LC-MS (method G): Rt 2.02 min; mass calcd. for C32H32F2N4O4 574.2, m/z found 575.2 [M+H]+; [α]D20 -32.75° (c 0.256, DMF).
266 (142 mg. 87%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 70:30 to 0:100). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.83 (t, J=5.9 Hz, 1H), 8.54 (s, 1H), 8.12 (d, J=5.3 Hz, 1H), 8.03 (d, J=1.5 Hz, 1H), 7.99 (dd, J=7.6, 5.6 Hz, 2H), 7.60 (d, J 1.1 Hz, 1H), 7.55 (t, J=54.4 Hz, 1H), 7.30 - 7.38 (m, 2H), 7.28 (s, 1H), 5.67 (s, 1H), 4.25 - 4.31 (m, 1H), 4.16 (dd, J=13.9, 5.7 Hz, 1H), 4.11 (s, 3H), 1.55 (s, 3H), 1.48 (s, 3H); LC-MS (method B): Rt 1.09 min; mass calcd. for C28H23F7N4O4 612.0, m/z found 613.0 [M+H]+; [α]D20 -82.05° (c 0.254, DMF).
267(137 mg. 86%) was synthesized according to procedure A. A purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6, 81° C.) δ ppm 8.58 (br t, J=5.8 Hz, 1H), 8.45 (s, 1H), 8.31 (s, 1H), 7.96 (d, J=1.3 Hz, 1H), 7.64 - 7.69 (m, 2H), 7.57 (d, J=1.3 Hz, 1H), 7.48 (t, J=54.5 Hz, 1H), 7.19 - 7.26 (m, 2H), 6.94 -7.10 (m, 1H), 4.26 (dd, J=14.2, 6.5 Hz, 1H), 4.13 (s, 3H), 4.07 - 4.12 (m, 1H), 1.65 (s, 3H), 1.60 (s, 3H); LC-MS (method E): Rt 2.13 min; mass calcd. for C28H23ClF6N4O4 628.0, m/z found 629.0 [M+H]+; [α]D20 -46.74° (c 0.261, DMF).
268 (121 mg. 72%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.71 (t, J=5.9 Hz, 1H), 8.54 (s, 1H), 8.15 (s, 1H), 8.00 (d, J=1.3 Hz, 1H), 7.65 - 7.71 (m, 2H), 7.60 (d, J=1.3 Hz, 1H), 7.56 (t, J=54.4 Hz, 1H), 7.20 - 7.27 (m, 2H), 5.59 (s, 1H), 5.41 (s, 1H), 4.13 (s, 3H), 3.84 - 3.96 (m, 2H), 1.64 (s, 3H), 1.56 (s, 3H), 1.49 - 1.55 (m, 1H), 0.55 - 0.63 (m, 1H), 0.36 - 0.45 (m, 1H), 0.28 - 0.36 (m, 1H), 0.12 - 0.21 (m, 1H); LC-MS (method B): Rt 1.10 min; mass calcd. for C30H28ClF3N4O4 600.0, m/z found 601.0 [M+H]+; [α]D20 -42.93° (c 0.410, DMF).
269 (121 mg. 72%) was synthesized according to procedure A. The purification was performed via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3CN, gradient from 80:20 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.71 (t, J=5.9 Hz, 1H), 8.53 (s, 1H), 8.02 (d, J 1.1 Hz, 1H), 7.93 - 8.00 (m, 3H), 7.60 (d, J 1.1 Hz, 1H), 7.55 (t, J 54.4 Hz, 1H), 7.26 - 7.33 (m, 2H), 5.58 (s, 1H), 5.42 (s, 1H), 4.11 (s, 3H), 3.89 - 3.98 (m, 2H), 1.56 - 1.63 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 0.56 - 0.64 (m, 1H), 0.38 - 0.46 (m, 1H), 0.28 - 0.36 (m, 1H), 0.13 - 0.22 (m, 1H); LC-MS (method E): Rt 2.08 min; mass calcd. for C30H28F4N4O4 584.0, m/z found 585.0 [M+H]+; [α]D20 -24.46° (c 0.254, DMF).
270 (153 mg. 51%) was synthesized according to procedure B. The precipitate was dissolved in CH2Cl2 and washed with water (twice). The organic layer was dried (MgSOa). The solids were removed by filtration and the filtrate was evaporated under reduced pressure. The residue was crystallized from DIPE and CH3CN (5:1), collected by filtration and dried under vacuum. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.68 (t, J=6.1 Hz, 1H), 8.53 (s, 1H), 8.02 (d, J=1.2 Hz, 1H), 7.92 - 8.00 (m, 2H), 7.77 - 7.83 (m, 1H), 7.60 (d, J=1.2 Hz, 1H), 7.48 - 7.56 (m, 1H), 7.55 (t, J=54.3 Hz, 1H), 5.59 (s, 1H), 5.41 (s, 1H), 4.11 (s, 3H), 3.96 - 4.02 (m, 1H), 3.86 - 3.93 (m, 1H), 1.56 - 1.64 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 0.58 - 0.65 (m, 1H), 0.39 - 0.47 (m, 1H), 0.28 -0.37 (m, 1H), 0.14 - 0.22 (m, 1H); LC-MS (method H): Rt 2.06 min; mass calcd. for C30H27F5N4O4 602.2, m/z found 603.2 [M+H]+; [α]D20 -34.65° (c 0.329, DMF).
271 (270 mg. 16%) was synthesized according to procedure A. The reaction was quenched with ice and water, and the mixture was stirred for 15 min. The precipitate was collected by filtration and washed with water. The residual fraction was dissolved in EtOAc and dried (MgSOi). The solids were removed by filtration and the filtrate was concentrated under reduced pressure. The crude product was crystallized from EtOAc, the precipitate was collected by filtration, washed with EtOAc and dried under vacuum at 65° C. The product was purified via reverse phase HPLC (stationary phase: Kromasil C18 100A 5 µm (Eka Nobel), mobile phase: NH4HCO3 (0.25% in H2O)/CH3OH, gradient from 90:10 to 20:80). 1H NMR (400 MHz, DMSO-d6) δ ppm 8.62 (t, J=6.1 Hz, 1H), 7.98 (dd, J=7.5, 5.5 Hz, 2H), 7.94 (d, J=5.7 Hz, 1H), 7.85 (s, 1H), 7.74 (d, J=1.3 Hz, 1H), 7.35 (d, J=1.5 Hz, 1H), 7.27 - 7.34 (m, 2H), 5.56 (s, 1H), 5.44 (s, 1H), 4.04 (s, 3H), 3.86 - 3.98 (m, 2H), 1.55 - 1.61 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 1.14 - 1.24 (m, 5H), 0.53 -0.63 (m, 1H), 0.36 - 0.45 (m, 1H), 0.25 - 0.35 (m, 1H), 0.11 - 0.21 (m, 1H); [α]D20 -32.40° (c 0.250, DMF).
The following compounds 273-336 were prepared using methods analogous to those described in the preceding examples.
1H NMR (400 MHz, DMSO-d6) δ 8.74 (t, J=5.72 Hz, 1H), 8.31 (d, J=9.90 Hz, 1H), 8.12 (d, J=5.50 Hz, 1H), 8.00 (dd, J=5.61, 7.59 Hz, 2H), 7.87 (d, J=1.54 Hz, 1H), 7.43 (d, J=1.32 Hz, 1H), 7.38-7.42 (m, 1H), 7.27-7.38 (m, 2H), 5.66 (s, 1H), 4.05-4.30 (m, 2H), 3.95 (s, 3H), 2.40 (s, 3H), 1.55 (s, 3H), 1.49 (s, 3H)
1H NMR (400 MHz, DMSO-d6) 6 ppm 1.53 (s, 6 H) 2.86 (s, 3 H) 4.06 (s, 3 H) 4.13 - 4.31 (m, 2 H) 5.71 (s, 1 H) 7.33 (s, 1 H) 7.39 (d, J=1.32 Hz, 1 H) 7.78 (d, J=1.54 Hz, 1 H) 7.88 (d, J=8.36 Hz, 2 H) 7.95 (s, 1 H) 8.15 (d, J=8.14 Hz, 2 H) 8.20 (d, J=5.50 Hz, 1 H) 8.78 (t, J=6.05 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6) 6 8.65 (t, J=6.10 Hz, 1H), 7.95-8.00 (m, 2H), 7.94 (d, J=5.70 Hz, 1H), 7.89 (s, 1H), 7.76 (d, J=1.22 Hz, 1H), 7.39 (d, J=1.22 Hz, 1H), 7.28-7.36 (m, 2H), 5.56 (s, 1H), 5.45 (s, 1H), 4.06 (s, 3H), 3.87-3.97 (m, 2H), 2.86 (s, 3H), 1.55-1.61 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 0.55-0.62 (m, 1H), 0.27-0.45 (m, 2H), 0.12-0.20 (m, 1H)
1H NMR (400 MHz, DMSO-d6) 6 8.98-9.12 (m, 1H), 8.87 (s, 1H), 8.33 (s, 1H), 8.07 (d, J=1.54 Hz, 1H), 7.65-7.77 (m, 3H), 7.48-7.64 (m, 1H), 7.15-7.37 (m, 2H), 5.68 (s, 1H), 4.15 (s, 3H), 4.08-4.30 (m, 2H), 1.64 (s, 3H), 1.56 (s, 3H)
LC-MS (RT: 1.15, MW = 647 [M+H]+, METHOD L)
1H NMR (400 MHz, DMSO-d6) 6 8.65 (t, J=5.94 Hz, 1H), 7.91-8.00 (m, 3H), 7.88 (s, 1H), 7.75 (d, J=1.32 Hz, 1H), 7.54 (d, J=7.65 Hz, 2H), 7.39 (d, J=1.32 Hz, 1H), 5.58 (s, 1H), 5.44 (s, 1H), 4.06 (s, 3H), 3.84-3.98 (m, 2H), 2.87 (s, 3H), 1.55-1.62 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 0.54-0.63 (m, 1H), 0.36-0.45 (m, 1H), 0.27-0.36 (m, 1H), 0.12-0.21 (m, 1H)
OR = -37.99 ° (589 nm, c 0.5475 w/v %, DMF, 20° C.)
1H NMR (400 MHz, DMSO-d6, 27° C.) δ ppm 8.69 (t, J=6.1 Hz, 1 H) 8.10 - 8.21 (m, 3 H) 7.83 (s, 1 H) 7.66 (d, J=1.3 Hz, 1 H) 7.31 - 7.41 (m, 2 H) 7.25 (d, J=1.3 Hz, 1 H) 7.04 (s, 1 H) 5.63 (s, 1 H) 4.49 - 4.62 (m, 1 H) 3.99 - 4.05 (m, 1 H) 3.98 (s, 3 H) 2.83 (s, 3 H) 1.50 (s, 6 H)
LC-MS(RT: 1.01, Area %: 98.37, MW: 576.00, BPM1: 577, BPM2: 577, METHOD L)
1H NMR (400 MHz, DMSO-d6, 27° C.) δ ppm 8.69 (t, J=6.1 Hz, 1 H) 8.10 - 8.21 (m, 3 H) 7.83 (s, 1 H) 7.66 (d, J=1.3 Hz, 1 H) 7.31 - 7.41 (m, 2 H) 7.25 (d, J=1.3 Hz, 1 H) 7.04 (s, 1 H) 5.63 (s, 1 H) 4.49 - 4.62 (m, 1 H) 3.99 - 4.05 (m, 1 H) 3.98 (s, 3 H) 2.83 (s, 3 H) 1.50 (s, 6 H)
1H NMR (400 MHz, DMSO-d6, 27° C.) δ ppm 8.69 (t, J=6.1 Hz, 1 H) 8.10 - 8.21 (m, 3 H) 7.83 (s, 1 H) 7.66 (d, J=1.3 Hz, 1 H) 7.31 - 7.41 (m, 2 H) 7.25 (d, J=1.3 Hz, 1 H) 7.04 (s, 1 H) 5.63 (s, 1 H) 4.49 - 4.62 (m, 1 H) 3.99 - 4.05 (m, 1 H) 3.98 (s, 3 H) 2.83 (s, 3 H) 1.50 (s, 6 H)
LC-MS(RT: 1.01, Area %: 98.37, MW: 576.00, BPM1: 577, BPM2: 577, METHOD L)
1H NMR (400 MHz, DMSO-d6) δ ppm 0.06 - 0.65 (m, 4 H) 1.10 - 1.28 (m, 4 H) 1.48 - 1.54 (m, 1 H) 1.56 (s, 3 H) 1.64 (s, 3 H) 3.79 - 3.98 (m, 2 H) 4.06 (s, 3 H) 5.43 (s, 1 H) 5.58 (s, 1 H) 7.25 (t, J=8.88 Hz, 2 H) 7.35 (d, J=1.25 Hz, 1 H) 7.62 - 7.71 (m, 2 H) 7.72 (d, J=1.25 Hz, 1 H) 7.87 (s, 1 H) 8.15 (s, 1 H) 8.64 (s, 1 H)
1H NMR (400 MHz, DMSO-d6) δ 8.37 (br t, J=5.72 Hz, 1H), 7.92-7.98 (m, 3H), 7.76 (s, 1H), 7.62 (d, J=1.54 Hz, 1H), 7.26-7.32 (m, 2H), 7.25 (d, J=1.32 Hz, 1H), 5.54 (d, J=11.22 Hz, 2H), 4.00 (s, 3H), 3.95-4.00 (m, 1H), 3.74 (dd, J=4.51, 13.31 Hz, 1H), 2.40-2.48 (m, 2H), 1.52 (s, 3H), 1.44 (s, 3H), 1.12-1.24 (m, 4H), 1.02 (d, J=6.82 Hz, 3H), 0.72 (d, J=6.82 Hz, 3H).
1H NMR (400 MHz, DMSO-d6) δ ppm 8.81 (s, 1 H) 8.75 (t, J=6.1 Hz, 1 H) 8.06 (d, J=1.5 Hz, 1 H) 7.92 - 8.00 (m, 3 H) 7.69 (d, J=1.3 Hz, 1 H) 7.20 - 7.34 (m, 2 H) 5.57 (s, 1 H) 5.41 (s, 1 H) 4.13 (s, 3 H) 3.94 (d, J=5.9 Hz, 2 H) 1.57 - 1.63 (m, 1 H) 1.54 (s, 3 H) 1.48 (s, 3 H) 0.55 - 0.65 (m, 1 H) 0.38 - 0.48 (m, 1 H) 0.26 - 0.36 (m, 1 H) 0.12 - 0.24 (m, 1 H)
1H NMR (400 MHz, DMSO-d6) δ 8.42-8.48 (m, 2H), 7.90-7.99 (m, 4H), 7.50 (d, J=1.32 Hz, 1H), 7.55 (t, J=54.36 Hz, 1H), 7.27 (t, J=8.25 Hz, 2H), 5.56 (s, 1H), 5.51 (s, 1H), 4.07 (s, 3H), 4.01 (dd, J=7.04, 13.42 Hz, 1H), 3.76 (dd, J=4.73, 13.31 Hz, 1H), 2.41-2.49 (m, 1H), 1.52 (s, 3H), 1.44 (s, 3H), 1.03 (d, J=6.82 Hz, 3H), 0.73 (d, J=6.82 Hz, 3H)
1H NMR (400 MHz, DMSO-d6, 27° C.) δ ppm 8.76 (t, J=5.9 Hz, 1 H) 8.12 (d, J=5.3 Hz, 1 H) 7.99 (dd, J=7.5, 5.5 Hz, 2 H) 7.88 (s, 1 H) 7.75 (d, J=1.5 Hz, 1 H) 7.31 - 7.40 (m, 3 H) 7.29 (s, 1 H) 5.67 (s, 1 H) 4.06 - 4.33 (m, 2 H) 4.05 (s, 3 H) 2.42 - 2.49 (m, 1 H) 1.55 (s, 3 H) 1.48 (s, 3 H) 1.12 - 1.24 (m, 4 H)
1H NMR (400 MHz, DMSO-d6) δ 8.62 (t, J=6.90 Hz, 1H), 8.42 (s, 1H), 7.88 (d, J=1.32 Hz, 1H), 7.76-7.87 (m, 2H), 7.45 (d, J=1.32 Hz, 1H), 7.53 (t, J=54.36 Hz, 1H), 7.21-7.31 (m, 2H), 5.57 (s, 1H), 5.32 (s, 1H), 4.03 (s, 3H), 3.60-4.29 (m, 2H), 1.67-1.75 (m, 1H), 1.64 (s, 6H), 0.65-0.76 (m, 1H), 0.40-0.53 (m, 1H), 0.19-0.36 (m, 2H)
1H NMR (400 MHz, DMSO-d6) δ 8.52 (t, J=6.80 Hz, 1H), 7.85 (dd, J=5.50, 7.48 Hz, 2H), 7.74 (s, 1H), 7.59 (d, J=1.32 Hz, 1H), 7.24-7.32 (m, 2H), 7.20 (d, J=1.54 Hz, 1H), 5.56 (s, 1H), 5.31 (s, 1H), 3.96 (s, 3H), 3.57-4.28 (m, 2H), 2.40-2.48 (m, 1H), 1.65-1.73 (m, 1H), 1.63 (s, 6H), 1.10-1.22 (m, 4H), 0.64-0.74 (m, 1H), 0.39-0.50 (m, 1H), 0.18-0.35 (m, 2H)
1H NMR (400 MHz, DMSO-d6) δ 8.62 (t, J=5.94 Hz, 1H), 7.98 (dd, J=5.50, 7.48 Hz, 2H), 7.94 (d, J=5.72 Hz, 1H), 7.85 (s, 1H), 7.74 (d, J=1.32 Hz, 1H), 7.35 (d, J=1.54 Hz, 1H), 7.18-7.34 (m, 2H), 5.56 (s, 1H), 5.44 (s, 1H), 3.84-3.99 (m, 2H), 2.41-2.48 (m, 1H), 1.55-1.62 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 1.12-1.26 (m, 4H), 0.54-0.64 (m, 1H), 0.36-0.48 (m, 1H), 0.25-0.34 (m, 1H), 0.11-0.23 (m, 1H)
1H NMR (400 MHz, DMSO-d6) δ 8.65 (t, J=1.00 Hz, 1H), 7.98 (dd, J=5.50, 7.48 Hz, 2H), 7.94 (d, J=5.72 Hz, 1H), 7.89 (s, 1H), 7.76 (d, J=1.54 Hz, 1H), 7.39 (d, J=1.54 Hz, 1H), 7.26-7.36 (m, 2H), 5.56 (br s, 1H), 5.45 (br s, 1H), 3.83-3.99 (m, 2H), 2.86 (s, 3H), 1.55-1.62 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H), 0.54-0.65 (m, 1H), 0.35-0.48 (m, 1H), 0.26-0.34 (m, 1H), 0.11-0.22 (m, 1H)
1HNMR (400 MHz, METHANOL-d4) δ 8.14 (t, J=6.55 Hz, 2H), 7.98 (d, J=1.32 Hz, 2H), 7.86 (s, 1H), 7.64 (br s, 2H), 7.20 (t, J=8.20 Hz, 2H), 4.61-4.69 (m, 1H), 4.02 (br d, J=13.86 Hz, 2H), 2.90 (br s, 3H), 1.55 (s, 3H), 1.55 (s, 3H), 0.89 (br s, 4H)
LC-MS(RT: 2.01, MW = 585 [M+H]+, METHOD Q)
1H NMR (400 MHz, DMSO-d6) δ 8.75 (t, J=6.16 Hz, 1H), 8.45 (s, 1H), 8.04 (d, J=5.50 Hz, 1H), 7.96 (d, J=1.32 Hz, 1H), 7.92 (dd, J=5.50, 7.70 Hz, 2H), 7.55 (s, 1H), 7.39-7.72 (m, 1H), 7.17-7.27 (m, 2H), 6.55 (s, 1H), 6.51-6.89 (m, 1H), 5.63 (s, 1H), 4.09 (s, 3H), 3.79-4.05 (m, 2H), 1.54 (s, 3H), 1.45 (s, 3H)
1H NMR (400 MHz, DMSO-d6) δ 8.66 (t, J=6.16 Hz, 1H), 8.03 (d, J=5.72 Hz, 1H), 7.93 (dd, J=5.50, 7.48 Hz, 2H), 7.79 (s, 1H), 7.69 (d, J=1.54 Hz, 1H), 7.30 (d, J=1.1.54 Hz, 1H), 7.19-7.28 (m, 2H), 6.55 (s, 1H), 6.48-6.83 (m, 1H), 5.62 (s, 1H), 4.02 (s, 3H), 3.77-4.00 (m, 2H), 2.43-2.48 (m, 1H), 1.54 (s, 3H), 1.45 (s, 3H), 1.13-1.25 (m, 4H)
1H NMR (400 MHz, DMSO-d6 ) d 8.72 (t, J=6.05 Hz, 1H), 7.92-8.05 (m, 3H), 7.87 (s, 1H), 7.78 (d, J=1.54 Hz, 1H), 7.41 (d, J=1.32 Hz, 1H), 7.26-7.35 (m, 2H), 5.55 (s, 1H), 5.46 (s, 1H), 4.07 (s, 3H), 3.69-3.91 (m, 2H), 2.87 (s, 3H), 1.52 (s, 6H), 1.39-1.47 (m, 1H), 0.96 (s, 3H), 0.79 (s, 3H), 0.69-0.76 (m, 1H), 0.38-0.46 (m, 1H)
LC-MS (RT: 1.12, MW =577 [M+H]+, METHOD L)
1H NMR (400 MHz, DMSO-d6 ) δ 8.73 (t, J=5.94 Hz, 1H), 7.94-8.02 (m, 3H), 7.87 (s, 1H), 7.78 (d, J=1.54 Hz, 1H), 7.41 (d, J=1.32 Hz, 1H), 7.26-7.37 (m, 2H), 5.55 (s, 1H), 5.47 (s, 1H), 4.07 (s, 3H), 3.68-3.90 (m, 2H), 2.87 (s, 3H), 1.52 (s, 6H), 1.44 (dd, J=5.83, 8.91 Hz, 1H), 0.96 (s, 3H), 0.79 (s, 3H), 0.69-0.76 (m, 1H), 0.42 (dd, J=3.85, 8.69 Hz, 1H)
LC-MS (RT: 1.12, MW = 577 [M+H]+, METHOD L)
1H NMR (400 MHz, DMSO-d6) δ 8.64 (t, J=5.72 Hz, 1H), 8.03 (d, J=5.50 Hz, 1H), 7.99 (dd, J=5.61, 7.59 Hz, 2H), 7.92 (s, 1H), 7.78 (d, J=1.54 Hz, 1H), 7.40 (d, J=1.32 Hz, 1H), 7.28-7.37 (m, 2H), 6.18 (s, 1H), 5.61 (s, 1H), 4.08-4.29 (m, 2H), 4.06 (s, 3H), 2.86 (s, 3H), 1.55 (s, 3H), 1.51 (s, 3H), 0.88-1.14 (m, 4H)
1H NMR (400 MHz, DMSO-d6 )δ8.51 (t, J=5.61 Hz, 1H), 7.96-8.03 (m, 3H), 7.95 (d, J=5.72 Hz, 1H), 7.81 (d, J=1.32 Hz, 1H), 7.42 (d, J=1.32 Hz, 1H), 7.27-7.39 (m, 2H), 5.66 (s, 1H), 5.58 (s, 1H), 4.07 (s, 3H), 3.99-4.36 (m, 2H), 2.87 (s, 3H), 1.56 (s, 3H), 1.52 (s, 3H), 1.04 (s, 3H), 0.90-0.99 (m, 1H), 0.77-0.86 (m, 1H), 0.20-0.29 (m, 1H), 0.00-0.06 (m, 1H)
LC-MS (RT: 1.93, MW = 563 [M+H]+, METHOD O)
1H NMR (400 MHz, DMSO-d6 )δ8.51 (t, J=5.61 Hz, 1H), 7.96-8.02 (m, 3H), 7.95 (d, J=5.50 Hz, 1H), 7.80 (d, J=1.32 Hz, 1H), 7.42 (d, J=1.32 Hz, 1H), 7.27-7.39 (m, 2H), 5.65 (s, 1H), 5.58 (s, 1H), 4.07 (s, 3H), 4.00-4.32 (m, 2H), 2.87 (s, 3H), 1.56 (s, 3H), 1.52 (s, 3H), 1.04 (s, 3H), 0.91-1.00 (m, 1H), 0.81-0.85 (m, 1H), 0.20-0.28 (m, 1H), 0.01-0.07 (m, 1H)
LC-MS(RT: 1.93, MW = 563 [M+H]+, METHOD O)
1H NMR (400 MHz, DMSO-d6) δ 8.66 (t, J=5.94 Hz, 1H), 7.93-8.02 (m, 3H), 7.86 (s, 1H), 7.75 (d, J=, 1H), 7.38 (d, J=1.32 Hz, 1H), 7.26-7.35 (m, 2H), 5.67 (s, 1H), 5.58 (s, 1H), 4.42-4.70 (m, 1H), 4.05 (s, 3H), 3.75-3.89 (m, 2H), 2.86 (s, 3H), 2.08-2.25 (m, 1H), 1.55 (s, 3H), 1.47 (s, 3H), 1.00-1.13 (m, 2H)
SFC (RT: 5.50, Method SFC)
1H NMR (400 MHz, DMSO-d6) δ 8.76 (t, J=5.94 Hz, 1H), 7.93-8.03 (m, 2H), 7.87-7.92 (m, 2H), 7.79 (d, J=1.32 Hz, 1H), 7.41 (d, J=1.54 Hz, 1H), 7.23-7.37 (m, 2H), 5.68 (s, 1H), 5.57 (s, 1H), 4.67-4.93 (m, 1H), 4.06 (s, 3H), 3.84-4.05 (m, 2H), 2.87 (s, 3H), 2.01-2.16 (m, 1H), 1.54 (s, 3H), 1.47 (s, 3H)
1H NMR (400 MHz, DMSO-d6) δ ppm 0.07 - 0.68 (m, 4 H) 1.45 (d, J=1.05 Hz, 6 H) 1.61 (ddd, J=8.15, 5.33, 3.03 Hz, 1 H) 2.85 (s, 3 H) 3.80 - 4.11 (m, 5 H) 5.28 (s, 1 H) 5.46 (s, 1 H) 7.30 (t, J=8.88 Hz, 2 H) 7.38 (d, J=1.25 Hz, 1 H) 7.83 (d, J=1.25 Hz, 1 H) 7.86 (s, 1 H) 8.10 - 8.24 (m, 2 H) 8.63 (t, J=5.80 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6) δ ppm 0.06 - 0.64 (m, 4 H) 1.45 (d, J=1.05 Hz, 6 H) 1.55 - 1.65 (m, 1 H) 2.85 (s, 1 H) 3.86 - 4.06 (m, 5 H) 5.19 - 5.34 (m, 1 H) 5.28 (s, 1 H) 5.45 (s, 1 H) 7.30 (t, J=8.88 Hz, 2 H) 7.37 (d, J=1.36 Hz, 1 H) 7.72 (d, J=1.36 Hz, 1 H) 7.74 (d, J=1.46 Hz, 1 H) 7.82 (d, J=1.36 Hz, 1 H) 7.86 (s, 1 H) 8.14 - 8.21 (m, 2 H) 8.63 (t, J=5.75 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6 ) δ 8.36 (t, J=6.80 Hz, 1H), 8.03 (d, J=5.28 Hz, 1H), 7.98 (dd, J=5.61, 7.59 Hz, 2H), 7.91 (s, 1H), 7.66 (d, J=1.32 Hz, 1H), 7.31-7.38 (m, 2H), 7.27 (d, J=1.32 Hz, 1H), 5.95 (s, 1H), 4.33-4.46 (m, 1H), 4.01 (s, 3H), 3.85-3.94 (m, 1H), 2.85 (s, 3H), 1.50 (s, 3H), 1.48 (s, 3H), 1.44 (d, J=1.00 Hz, 3H), 1.37 (d, J=22.01 Hz, 3H)
1H NMR (400 MHz, DMSO-d6 ) δ 8.34 (dd, J=4.73, 6.71 Hz, 1H), 8.03 (d, J=5.50 Hz, 1H), 7.98 (dd, J=5.50, 7.48 Hz, 2H), 7.89 (s, 1H), 7.65 (d, J=1.32 Hz, 1H), 7.30-7.39 (m, 2H), 7.27 (d, J=1.32 Hz, 1H), 5.97 (br s, 1H), 5.56 (br s, 1H), 4.33-4.43 (m, 1H), 4.01 (s, 3H), 3.86-3.96 (m, 1H), 2.84 (s, 3H), 1.50 (s, 3H), 1.48 (s, 3H), 1.44 (d, J=22.89 Hz, 3H), 1.37 (d, J=22.23 Hz, 3H)
1H NMR (400 MHz, DMSO-d6) δ 8.65 (t, J=6.16 Hz, 1H), 8.04 (d, J=5.50 Hz, 1H), 7.93 (dd, J=5.50, 7.70 Hz, 2H), 7.78 (s, 1H), 7.72 (d, J=1.54 Hz, 1H), 7.64 (d, J=1.32 Hz, 1H), 7.23-7.32 (m, 2H), 6.54 (s, 1H), 6.49-6.81 (m, 1H), 5.62 (s, 1H), 4.10 (tt, J=3.05, 5.97 Hz, 1H), 3.75-4.04 (m, 2H), 2.86 (s, 3H), 1.54 (s, 3H), 1.46 (s, 3H), 0.85-0.95 (m, 2H), 0.76-0.85 (m, 2H)
1HNMR (400 MHz, DMSO-d6 ) δ 8.53 (dd, J=4.40, 7.26 Hz, 1H), 8.16-8.25 (m, 2H), 7.92 (d, J=1.32 Hz, 1H), 7.83 (s, 1H), 7.79 (s, 1H), 7.65 (d, J=1.32 Hz, 1H), 7.30-7.36 (m, 2H), 7.25 (d, J=1.32 Hz, 1H), 6.57 (s, 1H), 5.32 (s, 1H), 4.39-4.49 (m, 1H), 3.98 (s, 3H), 3.90-3.96 (m, 1H), 2.83 (s, 3H), 1.60-1.77 (m, 3H), 1.43 (s, 6H)
1H NMR (400 MHz, DMSO-d6 ) δ 8.53 (dd, J=4.40, 7.04 Hz, 1H), 8.15-8.25 (m, 2H), 7.92 (d, J=1.10 Hz, 1H), 7.83 (s, 1H), 7.79 (s, 1H), 7.65 (d, J=1.32 Hz, 1H), 7.33 (t, J=8.80 Hz, 2H), 7.25 (d, J=1.10 Hz, 1H), 6.57 (s, 1H), 5.32 (s, 1H), 4.45 (dd, J=7.26, 13.64 Hz, 1H), 3.98 (s, 3H), 3.93 (dd, J=4.29, 13.75 Hz, 1H), 2.83 (s, 3H), 1.66 (t, J=19.48 Hz, 3H), 1.43 (s, 6H)
1H NMR (400 MHz, DMSO-d6) δ 8.79 (t, J=5.94 Hz, 1H), 8.12 (d, J=5.28 Hz, 1H), 7.99 (dd, J=5.72, 7.48 Hz, 2H), 7.93 (s, 1H), 7.78 (d, J=1.54 Hz, 1H), 7.39 (d, J=1.32 Hz, 1H), 7.32-7.38 (m, 2H), 7.29 (s, 1H), 5.67 (s, 1H), 4.11-4.30 (m, 2H), 4.06 (s, 3H), 1.55 (s, 3H), 1.48 (s, 3H)
1H NMR (400 MHz, DMSO-d6) δ 8.83 (t, J=6.05 Hz, 1H), 8.18 (d, J=1.32 Hz, 1H), 8.11 (d, J=5.28 Hz, 1H), 8.09 (s, 1H), 7.98 (dd, J=5.72, 7.70 Hz, 2H), 7.77 (s, 1H), 7.60 (br t, J=73.62 Hz, 1H), 7.30-7.38 (m, 2H), 7.23 (s, 1H), 5.66 (s, 1H), 4.12-4.31 (m, 2H), 2.92 (s, 3H), 1.55 (s, 3H), 1.49 (s, 3H)
LC-MS (RT: 1.13, MW = 613 [M+H]+, METHOD L)
1H NMR (400 MHz, DMSO-d6) δ 8.56 (t, J=5.80 Hz, 1H), 8.07 (d, J=5.28 Hz, 1H), 7.99 (dd, J=5.61, 7.59 Hz, 2H), 7.89 (s, 1H), 7.71 (d, J=1.54 Hz, 1H), 7.29-7.38 (m, 3H), 6.55 (s, 1H), 5.61 (s, 1H), 4.03 (s, 3H), 3.96-4.38 (m, 2H), 2.85 (s, 3H), 1.67 (t, J=19.59 Hz, 3H), 1.53 (s, 3H), 1.49 (s, 3H)
1H NMR (400 MHz, DMSO-d6 ) δ 8.56 (t, J=5.80 Hz, 1H), 8.07 (d, J=5.50 Hz, 1H), 7.94-8.02 (m, 2H), 7.89 (s, 1H), 7.71 (d, J=1.32 Hz, 1H), 7.27-7.40 (m, 3H), 6.55 (s, 1H), 5.61 (s, 1H), 4.03 (s, 3H), 3.95-4.38 (m, 2H), 2.85 (s, 3H), 1.61-1.75 (m, 3H), 1.53 (s, 3H), 1.49 (s, 3H)
1H NMR (400 MHz, DMSO-d6) δ ppm 0.12 - 0.20 (m, 1 H), 0.27 - 0.34 (m, 1 H), 0.40 (br dd, J=8.8, 5.3 Hz, 1 H), 0.55 - 0.62 (m, 1 H), 1.47 (s, 3 H), 1.54 (s, 3 H), 1.57 (s, 1 H), 2.86 (s, 3 H), 4.06 (s, 3 H), 5.43 (s, 1 H), 5.56 (s, 1 H), 7.28 - 7.35 (m, 2 H), 7.39 (d, J=1.3 Hz, 1 H), 7.76 (d, J=1.5 Hz, 1 H), 7.90 (s, 1 H), 7.92 - 8.01 (m, 3 H), 8.63 (s, 1 H)
1H NMR (400 MHz, DMSO-d6) 6 ppm 0.12 - 0.21 (m, 1 H), 0.27 - 0.45 (m, 2 H), 0.54 - 0.62 (m, 1 H), 1.47 (s, 3 H), 1.54 (s, 3 H), 1.55 - 1.61 (m, 1 H), 2.86 (s, 3 H), 4.06 (s, 3 H), 5.43 (s, 1 H), 5.56 (s, 1 H), 7.28 - 7.35 (m, 2 H), 7.40 (d, J=1.3 Hz, 1 H), 7.76 (d, J=1.3 Hz, 1 H), 7.90 (s, 1 H), 7.92 - 8.01 (m, 3 H), 8.63 (s, 1 H)
1H NMR (400 MHz, DMSO-d6) δ ppm 0.75 - 1.15 (m, 10 H) 1.54 (d, J=15.6 Hz, 6 H) 2.86 (s, 3 H) 3.99 - 4.39 (m, 2 H) 4.11 -4.12(m, 1 H) 5.61 (s, 1 H) 6.17 (s, 1 H) 7.24 - 7.41 (m, 2 H) 7.71 (d, J=1.5 Hz, 1 H) 7.80 (d, J=1.5 Hz, 1 H) 7.91 (s, 1 H) 7.94 - 8.10 (m, 3 H) 8.61 (t, J=5.7 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6) δ ppm 0.80 - 0.94 (m, 4 H) 1.52 (d, J=28.2 Hz, 5 H) 1.45 - 1.46 (m, 1 H) 2.86 (s, 3 H) 4.07 - 4.33 (m, 2 H) 4.12 - 4.13 (m, 1 H) 5.67 (s, 1 H) 7.29 (s, 1 H) 7.31 -7.41 (m, 2 H) 7.70 (d, J=1.5 Hz, 1 H) 7.79 (d, J=1.5 Hz, 1 H) 7.91 (s, 1 H) 8.00 (dd, J=7.6, 5.6 Hz, 2 H) 8.12 (d, J=5.5 Hz, 1 H) 8.16 - 8.19 (m, 1 H) 8.75 (t, J=5.9 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6) δ ppm 1.50 (d, J=31.7 Hz, 7 H) 2.58 - 2.61 (m, 1 H) 2.87 (s, 3 H) 3.84 - 4.04 (m, 2 H) 4.08 (s, 3 H) 5.59 (s, 1 H) 5.85 (s, 1 H) 7.29 - 7.40 (m, 2 H) 7.44 (d, J=1.5 Hz, 1 H) 7.81 (d, J=1.3 Hz, 1 H) 7.88 - 8.11 (m, 4 H) 8.87 (t, J=6.1 Hz, 2 H) 8.91 - 8.94 (m, 1 H)
LC-MS(RT: 2.02, MW = 585 [M+H]+, METHOD P)
1H NMR (400 MHz, DMSO-d6) 6 ppm 1.45 - 1.83 (m, 1 H) 1.48 - 1.58 (m, 6 H) 1.70 - 1.82 (m, 1 H) 2.53 - 2.64 (m, 1 H) 2.87 (s, 3 H) 3.74 - 3.93 (m, 2 H) 3.76 - 3.82 (m, 1 H) 4.06 (s, 3 H) 5.55 - 5.64 (m, 1 H) 5.60 (s, 1 H) 5.81 - 5.94 (m, 1 H) 5.88 (s, 1 H) 7.27 - 7.36 (m, 1 H) 7.37 -7.42 (m, 1 H) 7.74 - 7.80 (m, 1 H) 7.83 - 7.87 (m, 1 H) 7.92 - 8.02 (m, 1 H) 7.98 (s, 1 H) 8.77 (t, J=6.1 Hz, 1 H)
LC-MS (RT: 1.93, MW = 585 [M+H]+, METHOD P)
1H NMR (400 MHz, DMSO-d6) δ ppm 0.83 - 0.89 (m, 2 H) 1.06 - 1.13 (m, 2 H) 1.46 - 1.58 (m, 6 H) 2.08 - 2.21 (m, 1 H) 2.08 - 2.21 (m, 1 H) 3.95 (s, 3 H) 4.09 - 4.33 (m, 2 H) 5.67 (s, 1 H) 7.30 - 7.41 (m, 4 H) 7.78 (d, J=1.54 Hz, 1 H) 7.88 (d, J=2.20 Hz, 1 H) 8.00 (dd, J=7.59, 5.61 Hz, 2 H) 8.13 (d, J=5.50 Hz, 1 H) 8.67 (t, J=5.83 Hz, 1 H) 8.75 (d, J=2.20 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6) δ 8.77 (d, J=1.98 Hz, 1H), 8.72 (br t, J=5.83 Hz, 1H), 8.13 (d, J=5.50 Hz, 1H), 8.04 (s, 1H), 7.94-8.03 (m, 2H), 7.80 (s, 1H), 7.42 (s, 1H), 7.27-7.40 (m, 3H), 5.66 (s, 1H), 4.10-4.36 (m, 2H), 3.95 (s, 3H), 2.48 (s, 3H), 1.55 (s, 3H), 1.49 (s, 3H)
1H NMR (400 MHz, DMSO-d6) δ 8.75 (s, 1H), 8.68 (t, J=6.01 Hz, 1H), 8.14 (d, J=5.28 Hz, 1H), 7.98-8.05 (m, 3H), 7.81 (d, J=1.54 Hz, 1H), 7.67 (d, J=1.54 Hz, 1H), 7.40 (s, 1H), 7.32-7.39 (m, 2H), 5.67 (s, 1H), 4.11-4.31 (m, 2H), 4.01 (tt, J=2.92, 6.00 Hz, 1H), 2.46-2.49 (m, 3H), 1.56 (s, 3H), 1.49 (s, 3H), 0.81-0.89 (m, 2H), 0.72-0.81 (m, 2H)
1H NMR (400 MHz, DMSO-d6) δ ppm 1.41 - 1.60 (m, 6 H) 3.98 (s, 3 H) 4.12 - 4.31 (m, 2 H) 5.68 (d, J=47.32 Hz, 2 H) 5.66 (s, 1 H) 7.30 - 7.41 (m, 3 H) 7.46 (d, J=1.32 Hz, 1 H) 7.93 (d, J=1.54 Hz, 1 H) 8.00 (dd, J=7.70, 5.50 Hz, 2 H) 8.13 (d, J=5.28 Hz, 1 H) 8.34 - 8.40 (m, 1 H) 8.74 (t, J=5.72 Hz, 1 H) 8.92 - 8.97 (m, 1 H)
1H NMR (400 MHz, DMSO-d6) δ ppm 1.43 - 1.60 (m, 6 H) 4.00 (s, 3 H) 4.10 - 4.33 (m, 2 H) 5.66 (s, 1H) 7.18 - 7.48 (m, 1 H) 7.31 - 7.37 (m, 4 H) 7.52 (d, J=1.32 Hz, 1 H) 7.96 - 8.03 (m, 3 H) 8.13 (d, J=5.50 Hz, 1 H) 8.58 (d, J=1.76 Hz, 1 H) 8.75 (t, J=5.94 Hz, 1 H) 9.06 (d, J=2.20 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6) δ ppm 1.45 - 1.56 (m, 6 H) 4.01 (s, 3 H) 4.10 - 4.35 (m, 2 H) 5.67 (s, 1 H) 7.27 - 7.38 (m, 3 H) 7.58 (d, J=1.54 Hz, 1 H) 7.99 (dd, J=7.59, 5.61 Hz, 2 H) 8.07 (d, J=1.32 Hz, 1 H) 8.13 (d, J=5.28 Hz, 1 H) 8.76 (t, J=5.83 Hz, 1 H) 8.87 (d, J=1.32 Hz, 1 H) 9.19 (d, J=2.20 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6) δ 9.07 (d, J=1.98 Hz, 1H), 8.76 (t, J=5.83 Hz, 1H), 8.60 (d, J=1.54 Hz, 1H), 8.34 (s, 1H), 8.00 (d, J=1.54 Hz, 1H), 7.65-7.74 (m, 2H), 7.52 (d, J=1.32 Hz, 1H), 7.35 (d, J=9.68 Hz, 1H), 7.25-7.33 (m, 2H), 7.20 (t, J=1.00 Hz, 1H), 5.69 (s, 1H), 4.09-4.30 (m, 2H), 4.01 (s, 3H), 1.65 (s, 3H), 1.57 (s, 3H)
1H NMR (400 MHz, DMSO-d6) δ 9.20 (d, J=2.20 Hz, 1H), 8.89 (d, J=1.32 Hz, 1H), 8.77 (t, J=5.94 Hz, 1H), 8.34 (s, 1H), 8.06 (d, J=1.54 Hz, 1H), 7.66-7.74 (m, 2H), 7.58 (d, J=1.32 Hz, 1H), 7.36 (s, 1H), 7.20-7.32 (m, 2H), 5.69 (s, 1H), 4.09-4.29 (m, 2H), 4.03 (s, 3H), 1.65 (s, 3H), 1.58 (s, 3H)
1H NMR (400 MHz, DMSO-d6) δ 9.18 (d, J=2.20 Hz, 1H), 8.84 (d, J=1.32 Hz, 1H), 8.61 (t, J=5.83 Hz, 1H), 8.06 (d, J=1.54 Hz, 1H), 7.93-8.00 (m, 3H), 7.58 (d, J=1.54 Hz, 1H), 7.23-7.35 (m, 2H), 5.57 (s, 1H), 5.48 (s, 1H), 4.01 (s, 3H), 3.84-3.99 (m, 2H), 1.55-1.63 (m, 1H), 1.54 (s, 3H), 1.48 (s, 3H), 0.53-0.67 (m, 1H), 0.37-0.47 (m, 1H), 0.26-0.36 (m, 1H), 0.11-0.23 (m, 1H)
1H NMR (400 MHz, DMSO-d6) δ ppm 1.48 (s, 3 H) 1.56 (s, 3 H) 4.00 (s, 3 H) 4.23 (ddd, J=75.76, 13.81, 5.94 Hz, 2 H) 5.69 (s, 1 H) 7.34 (t, J=55.13 Hz, 1 H) 7.38 (s, 1 H) 7.50 - 7.63 (m, 2 H) 7.77 - 7.85 (m, 1 H) 7.93 - 8.04 (m, 2 H) 8.16 (d, J=5.28 Hz, 1 H) 8.59 (d, J=1.54 Hz, 1H) 8.74 (t, J=5.94 Hz, 1 H) 9.06 (d, J=1.98 Hz, 1 H)
1H NMR (400 MHz, DMSO-d6) δ ppm 0.11 - 0.21 (m, 1 H) 0.27 - 0.34 (m, 1 H) 0.35 - 0.45 (m, 1 H) 0.54 - 0.65 (m, 1 H) 1.47 - 1.54 (m, 1 H) 1.56 (s, 3 H) 1.64 (s, 3 H) 3.83 - 3.98 (m, 2 H) 4.02 (s, 3 H) 5.48 (s, 1 H) 5.58 (s, 1 H) 7.23 (m, J=8.90, 8.90 Hz, 2 H) 7.58 (d, J=1.54 Hz, 1 H) 7.68 (m, J=8.80, 5.70 Hz, 2 H) 8.04 (d, J=1.54 Hz, 1 H) 8.16 (s, 1 H) 8.64 (t, J=1.00 Hz, 1 H) 8.86 (d, J=1.32 Hz, 1 H) 9.19 (d, J=2.20 Hz, 1 H)
LC-MS (RT: 2.51, MW = 618 [M+H]+, METHOD U)
1H NMR (400 MHz, DMSO-d6) δ 8.74 (d, J=2.42 Hz, 1H), 8.51 (t, J=5.83 Hz, 1H), 7.98 (dd, J=5.50, 7.48 Hz, 2H), 7.94 (d, J=5.72 Hz, 1H), 7.86 (d, J=2.20 Hz, 1H), 7.76 (d, J=1.54 Hz, 1H), 7.26-7.36 (m, 3H), 5.56 (d, J=2.20 Hz, 2H), 3.94 (s, 3H), 3.84-4.01 (m, 2H), 2.10-2.20 (m, 1H), 1.54 (s, 3H), 1.51-1.62 (m, 1H), 1.47 (s, 3H), 1.06-1.14 (m, 2H), 0.82-0.91 (m, 2H), 0.53-0.62 (m, 1H), 0.35-0.45 (m, 1H), 0.26-0.34 (m, 1H), 0.15 (ddt, J=3.74, 5.56, 8.78 Hz, 1H)
1HNMR (400 MHz, DMSO-d6) δ 8.81 (t, J=5.94 Hz, 1H), 8.13 (d, J=5.50 Hz, 1H), 7.89-7.97 (m, 3H), 7.79 (d, J=1.54 Hz, 1H), 7.45-7.56 (m, 3H), 7.40 (d, J=1.32 Hz, 1H), 7.31 (s, 1H), 5.67 (s, 1H), 4.13-4.32 (m, 2H), 4.06 (s, 3H), 2.87 (s, 3H), 1.56 (s, 3H), 1.50 (s, 3H).
LC-MS (RT: 1.00, MW = 558 [M+H]+, METHOD L)
1HNMR (400 MHz, DMSO-d6) δ ppm 1.52 (d, J=27.5 Hz, 6 H) 2.87 (s, 3 H) 4.07 (s, 3 H) 4.11 - 4.21 (m, 1 H) 4.25 (br d, J=6.2 Hz, 1 H) 5.67 (s, 1 H) 7.29 (s, 1 H) 7.35 (t, J=8.8 Hz, 2 H) 7.39 (d, J=1.1 Hz, 1 H) 7.50 - 7.51 (m, 1 H) 7.78 (d, J=1.1 Hz, 1 H) 7.93 (s, 1 H) 8.00 (br dd, J=7.5, 5.7 Hz, 2 H) 8.13 (d, J=5.3 Hz, 1 H) 8.35 - 8.38 (m, 1 H) 8.79 (br t, J=5.8 Hz, 1 H)
1HNMR (400 MHz, DMSO-d6) δ ppm 0.74 - 1.17 (m, 9 H) 1.54 (d, J=15.6 Hz, 6 H) 2.86 (s, 3 H) 4.05 - 4.18 (m, 2 H) 4.26 (dd, J=13.4, 5.9 Hz, 1 H) 5.61 (s, 1 H) 6.17 (s, 1 H) 7.33 (t, J=8.9 Hz, 2 H) 7.71 (d, J=1.3 Hz, 1 H) 7.80 (d, J=1.3 Hz, 1 H) 7.91 (s, 1 H) 7.95 - 8.08 (m, 2 H) 8.02 -8.05 (m, 1 H) 8.61 (t, J=5.8 Hz, 1 H)
Absolute configuration of compound 95 has been confirmed to be S by use of X-Ray crystallography.
The single crystal was obtained by cooling in DMF followed by prolonged incubation at 5° C.
For the structure 95 as presented, with the stereocentre in the S configuration at C2 The Flack parameter = -0.05(6), (Acta Cryst. B69, 2013, 249-259).
Determination of the absolute structure using Bayesian statistics on Bijvoet differences, reveals that the probability of the absolute structure as presented being correct is 1.000, while the probabilities of the absolute structure being a racemic twin or false are both 0.000. The Flack equivalent and its uncertainty are calculated through this program to be = -0.05(5). The calculation was based on 1364 Bijvoet pairs with a coverage of 96% (Hooft et al., J. Appl. Cryst., 2008, 41, 96-103).
Black 384-well clear-bottom microtiter plates (Corning, Amsterdam, The Netherlands) were filled via acoustic drop ejection using the echo liquid handler (Labcyte, Sunnyvale, California). 200 nL of compound stock solutions (100% DMSO) were transferred to the assay plates. 9 serial 4-fold dilutions of compound were made, creating per quadrant the same compound concentration. The assay was initiated by adding 10 µL of culture medium to each well (RPMI medium without phenol red, 10% FBS-heat inactivated, 0.04% gentamycin (50 mg/mL). All addition steps are done by using a multidrop dispenser (Thermo Scientific, Erembodegem, Belgium). Next, rgRSV224 virus (MOI = 1) diluted in culture medium was added to the plates. rgRSV224 virus is an engineered virus that includes an additional GFP gene (Hallak LK, Spillmann D, Collins PL, Peeples ME. Glycosaminoglycan sulfation requirements for respiratory syncytial virus infection; Journal of virology (2000), 74(22), 10508-13) and was in-licensed from the NIH (Bethesda, MD, USA). Finally, 20 µL of a HeLa cell suspension (3,000 cells/well) were plated. Medium, virus- and mock-infected controls were included in each test. The wells contain 0.05% DMSO per volume. Cells were incubated at 37° C. in a 5% CO2 atmosphere. Three days post-virus exposure, viral replication was quantified by measuring GFP expression in the cells by an in house developed MSM laser microscope (Tibotec, Beerse, Belgium). The EC50 was defined as the 50% inhibitory concentration for GFP expression. In parallel, compounds were incubated for three days in a set of white 384-well microtiter plates (Corning) and the cytotoxicity of compounds in HeLa cells was determined by measuring the ATP content of the cells using the ATPlite kit (Perkin Elmer, Zaventem, Belgium) according to the manufacturer’s instructions. The CC50 was defined as the 50% concentration for cytotoxicity.
The antiviral properties of some of the compounds of the present application have been compared in the table below with one compound exemplified in WO-2015/026792 and with one compound (i.e. Compound A) that is encompassed by WO-2015/026792 but not exemplified therein.
Compound A has been specifically made to allow for this comparative testing as is structurally differs with compound (272) in the presence of R2 is methyl.
Compound 321, exemplified in WO-2015/026792, on page 211 has been resynthesized and tested in the antiviral RSV assay described in Example 5.1 to allow for a direct comparison in antiviral effect.
The antiviral data (EC50 values) against RSV in the table below demonstrate the unexpectedly improved antiviral properties against the respiratory syncytial virus (RSV) for the compounds that have a non-hydrogen substituent on the carbon bearing the R2 substituent.
“Active ingredient” as used throughout these examples relates to a final compound of Formula (I), the pharmaceutically acceptable salts thereof, the solvates and the stereochemically isomeric forms and the tautomers thereof.
Typical examples of recipes for the formulation of the invention are as follows:
In this Example, active ingredient can be replaced with the same amount of any of the compounds according to the present invention, in particular by the same amount of any of the exemplified compounds.
An aqueous suspension is prepared for oral administration so that each 1 milliliter contains 1 to 5 mg of one of the active compounds, 50 mg of sodium carboxymethyl cellulose, 1 mg of sodium benzoate, 500 mg of sorbitol and water ad 1 ml.
A parenteral composition is prepared by stirring 1.5 % by weight of active ingredient of the invention in 10% by volume propylene glycol in water.
In this Example, active ingredient can be replaced with the same amount of any of the compounds according to the present invention, in particular by the same amount of any of the exemplified compounds.
| Number | Date | Country | Kind |
|---|---|---|---|
| 20170578.7 | Apr 2020 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2021/060384 | 4/21/2021 | WO |
