Claims
- 1. An isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:
(a) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO: 3, wherein the % sequence identity is based on the entire coding region and is calculated by the GAP algorithm under default parameters, wherein the sequence encodes a polypeptide with RuvB activity; and (b) a nucleic acid sequence which is fully complementary to the nucleic acid sequence of (a).
- 2. The isolated polynucleotide of claim 1, wherein the nucleic acid sequence has at least 85% sequence identity to SEQ ID NO: 3.
- 3. The isolated polynucleotide of claim 1, wherein the nucleic acid sequence has at least 90% sequence identity to SEQ ID NO: 3.
- 4. A recombinant expression cassette, comprising the polynucleotide of claim 1 operably linked to a promoter.
- 5. A host cell comprising the polynucleotide of claim 1.
- 6. A transgenic plant comprising the polynucleotide of claim 1.
- 7. The transgenic plant of claim 6, wherein said plant is a monocot.
- 8. The transgenic plant of claim 6, wherein said plant is a dicot.
- 9. The transgenic plant of claim 6, wherein the plant is selected from the group consisting of corn, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, and millet.
- 10. A transgenic seed from the transgenic plant of claim 6, wherein the seed comprises the polynucleotide.
- 11. An isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:
(a) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity of the entire length of SEQ ID NO: 4, as determined by the GAP algorithm under default parameters, wherein the encoded polypeptide has RuvB activity; and, (b) a nucleic acid sequence which is fully complementary to the nucleic acid sequence of (a).
- 12. The isolated polynucleotide of claim 11, wherein the polynucleotide encodes a polypeptide having at least 85% sequence identity to SEQ ID NO: 4.
- 13. The isolated polynucleotide of claim 11, wherein the polynucleotide encodes a polypeptide having at least 90% sequence identity to SEQ ID NO: 4.
- 14. A recombinant expression cassette comprising the polynucleotide of claim 11 operably linked to a promoter.
- 15. A host cell comprising the polynucleotide of claim 11.
- 16. The host cell of claim 15, wherein the host cell is a plant cell.
- 17. A transgenic plant comprising the polynucleotide of claim 11.
- 18. The transgenic plant of claim 17, wherein said plant is a monocot.
- 19. The transgenic plant of claim 17, wherein said plant is a dicot.
- 20. The transgenic plant of claim 17, wherein said plant is selected from the group consisting of maize, soybean, safflower, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, and millet.
- 21. A transgenic seed from the transgenic plant of claim 17, wherein the seed comprises the polynucleotide.
- 22. A method of modulating the level of RuvB in a plant cell, comprising:
(a) introducing into a plant cell a recombinant expression cassette comprising the polynucleotide of claim 1 operably linked to a promoter; (b) culturing the plant cell under plant cell growing conditions; and (c) expressing the polynucleotide for a time sufficient to modulate the level of RuvB in the plant cell.
- 23. A method of modulating the level of RuvB in a plant, comprising:
(a) introducing into a plant cell a recombinant expression cassette comprising the polynucleotide of claim 1 operably linked to a promoter; (b) culturing the plant cell under plant cell growing conditions; (c) regenerating a transformed plant comprising the polynucleotide; and (d) expressing the polynucleotide for a time sufficient to modulate the level of RuvB in the plant.
- 24. The method of claim 23, wherein the plant is maize.
- 25. A method of modulating the level of RuvB in a plant cell, comprising:
(a) introducing into a plant cell a recombinant expression cassette comprising the polynucleotide of claim 11 operably linked to a promoter; (b) culturing the plant cell under plant cell growing conditions; and (c) expressing the polynucleotide for a time sufficient to modulate the level of RuvB in the plant cell.
- 26. A method of modulating the level of RuvB in a plant, comprising:
(a) introducing into a plant cell a recombinant expression cassette comprising the polynucleotide of claim 11 operably linked to a promoter; (b) culturing the plant cell under plant cell growing conditions; (c) regenerating a transformed plant comprising the polynucleotide; and (d) expressing the polynucleotide for a time sufficient to modulate the level of RuvB in the plant.
- 27. The method of claim 26, wherein the plant is maize.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. application Ser. No. 09/589,510 filed Jun. 7, 2000, and U.S. Application Serial No. 60/144,112 filed Jul. 16, 1999, both of which are herein incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60144112 |
Jul 1999 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09589510 |
Jun 2000 |
US |
Child |
10782435 |
Feb 2004 |
US |