Patent Application
20240025920
The present disclosure claims priority and benefit to the Chinese Patent Application No. 202011383418.7 filed with National Intellectual Property Administration, PRC on Nov. 30, 2020, the disclosure of which is incorporated herein by reference in its entirety.
The present disclosure is in the field of pharmaceutical chemistry, and particularly relates to a salt form as a Cdc7 inhibitor and crystal forms thereof, more particularly to a compound of formula (II) and a crystal form thereof, and also to a method for preparing the salt form and the crystal form and use thereof for preparing a medicament for preventing or treating tumors.
Cdc7 is a serine/threonine kinase that binds to ASK (activator of S phase kinase, also known as DBF4) in the nucleus. In one aspect, it activates MCM to promote the formation of replication origin complexes by phosphorylating minichromosome maintenance proteins (MCM proteins), an important element of DNA replication initiator. In another aspect, it also participates in the ATR/Chk1 pathway and serves as an important regulatory factor of the S-phase checkpoint of a cell cycle to promote cell cycle arrest and DNA repair. Cdc7 is abnormally highly expressed in various human tumor cells, and such abnormally high expression is shown to highly correlate with tumor proliferation and metastasis as well as resistance to chemotherapeutic drugs. Therefore, Cdc7 has now become an important marker and target in tumor research.
In one aspect, the present disclosure provides a compound of formula (II)
In another aspect, the present disclosure also provides a crystal form of the compound of formula (II).
In some embodiments of the present disclosure, the crystal form of the compound of formula (II) is selected from the group consisting of:
crystal form A of the compound of formula (II), an X-ray powder diffraction pattern of which comprises characteristic peaks at 2θ values of 11.46±0.20°, 24.03±0.20° and 25.16±0.20°;
crystal form B of the compound of formula (II), an X-ray powder diffraction pattern of which comprises characteristic peaks at 2θ values of 5.86±0.20°, 17.64±0.20° and 24.89±0.20°; and
crystal form C of the compound of formula (II), an X-ray powder diffraction pattern of which comprises characteristic peaks at 2θ values of 7.40±0.20°, 11.21±0.20° and 22.18±0.20°.
In another aspect, the present disclosure also provides crystal form A of the compound of formula (II), an X-ray powder diffraction pattern of which comprises characteristic peaks at 2θ values of 11.46±0.20°, 24.03±0.20° and 25.16±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form A described above using Cu Kα radiation has diffraction peaks at 2θ values of 11.46±0.20°, 24.03±0.20° and 25.16±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form A described above comprises characteristic peaks at 2θ values of 11.46±0.20°, 12.06±0.20°, 16.96±0.20°, 17.60±0.20°, 18.48±0.20°, 19.55±0.20°, 24.03±0.20° and 25.16±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form A described above comprises characteristic peaks at 2θ values of 6.46°±0.20°, 9.36°±0.20°, 11.46°±0.20°, 12.06°±0.20°, 12.39°±0.20°, 12.89°±0.20°, 13.37°±0.20°, 13.87°±0.20°, 14.09°±0.20°, 16.10°±0.20°, 16.96°±0.20°, 17.19°±0.20°, 17.60°±0.20°, 18.48°±0.20°, 18.75°±0.20°, 19.37°±0.20°, 19.55°±0.20°, 21.21°±0.20°, 21.65°±0.20°, 22.36°±0.20°, 23.06°±0.20°, 23.42°±0.20°, 23.74°±0.20°, 24.03°±0.20°, 25.16°±0.20°, 25.47°±0.20°, 26.59°±0.20°, 27.32°±0.20°, 28.11°±0.20°, 28.77°±0.20°, 29.73°±0.20°, 31.81°±0.20°, 33.09°±0.20°, 33.80°±0.20°, 34.19°±0.20°, 35.49°±0.20°, 35.99°±0.20°, 37.66°±0.20°, 38.14°±0.20°, 38.77°±0.20° and 39.38°±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form A described above comprises characteristic peaks at 2θ values of 6.46°, 9.36°, 11.46°, 12.06°, 12.39°, 12.89°, 13.37°, 13.87°, 14.09°, 16.10°, 16.96°, 17.19°, 17.60°, 18.48°, 18.75°, 19.37°, 19.55°, 21.21°, 21.65°, 22.36°, 23.06°, 23.42°, 23.74°, 24.03°, 25.16°, 25.47°, 26.59°, 27.32°, 28.11°, 28.77°, 29.73°, 31.81°, 33.09°, 33.80°, 34.19°, 35.49°, 35.99°, 37.66°, 38.14°, 38.77° and 39.38°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form A described above is shown in
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of crystal form A of the compound of formula (II) is shown in Table 1.
In some embodiments of the present disclosure, a DSC curve of the crystal form A described above has an endothermic peak starting point at 230.7° C.±3° C.
In some embodiments of the present disclosure, a DSC profile of the crystal form A described above is shown in
In some embodiments of the present disclosure, a TGA curve of the crystal form A described above shows a weight loss of 0.94±0.2% at 200.0° C.±3° C.
In some embodiments of the present disclosure, a TGA profile of the crystal form A described above is shown in
In some embodiments of the present disclosure, an infrared spectrum of the crystal form A described above comprises characteristic absorption peaks at 3033.88 cm−1±5 cm−1, 3225.69 cm−1±5 cm−1, 3087.88 cm−1±5 cm−1, 2945.00 cm−1±5 cm−1, 2925.62 cm−1±5 cm−1, 2837.85 cm−1±5 cm−1, 1680.20 cm−1±5 cm−1, 1662.75 cm−1±5 cm−1, 1582.38 cm−1±5 cm−1, 1551.77 cm−1±5 cm−1, 1475.47 cm−1±5 cm−1 and 1438.43 cm−1±5 cm−1.
In some embodiments of the present disclosure, an infrared absorption spectrum of the crystal form A described above is shown in
In some embodiments of the present disclosure, an ultraviolet absorption spectrum of the crystal form A described above has characteristic absorption peaks at 259.0 nm±3 nm, 214.4 nm±3 nm and 322.6 nm±3 nm.
In some embodiments of the present disclosure, an ultraviolet absorption spectrum of the crystal form A described above is shown in
In another aspect, the present disclosure also provides crystal form B of the compound of formula (II), an X-ray powder diffraction pattern of which comprises characteristic peaks at 2θ values of 5.86±0.20°, 17.64±0.20° and 24.89±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form B described above using Cu Kα radiation has diffraction peaks at 2θ values of 5.86±0.20°, 17.64±0.20° and 24.89±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form B described above comprises characteristic peaks at 2θ values of 5.86±0.20°, 10.17±0.20°, 11.73±0.20°, 15.83±0.20°, 17.64±0.20°, 23.59±0.20°, 24.89±0.20° and 25.80±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form B described above comprises characteristic peaks at 2θ values of 5.86°±0.20°, 10.17°±0.20°, 11.73°±0.20°, 12.57°±0.20°, 14.15°±0.20°, 14.40°±0.20°, 15.23°±0.20°, 15.83°±0.20°, 16.26°±0.20°, 16.71°±0.20°, 17.64°±0.20°, 18.04°±0.20°, 18.73°±0.20°, 19.99°±0.20°, 20.57°±0.20°, 21.08°±0.20°, 23.59°±0.20°, 24.36°±0.20°, 24.89°±0.20°, 25.41°±0.20°, 25.80°±0.20°, 27.20°±0.20°, 27.90°±0.20°, 28.90°±0.20°, 29.39°±0.20°, 29.70°±0.20°, 30.52°±0.20°, 30.81°±0.20°, 31.99°±0.20°, 34.29°±0.20°, 35.83°±0.20°, 39.64°±0.20°, 28.90°±0.20°, 29.39°±0.20°, 29.70°±0.20°, 30.52°±0.20°, 30.81°±0.20°, 31.99°±0.20°, 34.29°±0.20°, 35.83°±0.20° and 39.64°±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form B described above comprises characteristic peaks at 2θ values of 5.86°, 10.17°, 11.73°, 12.57°, 14.15°, 14.40°, 15.83°, 16.26°, 16.71°, 17.64°, 18.04°, 18.73°, 19.99°, 20.57°, 21.08°, 23.59°, 24.36°, 24.89°, 25.41°, 27.20°, 27.90°, 28.90°, 29.39°, 29.70°, 30.52°, 30.81°, 31.99°, 34.29°, 35.83°, 39.64°, 28.90°, 29.39°, 29.70°, 30.52°, 30.81°, 31.99°, 34.29°, 35.83° and 39.64°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form B described above is shown in
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of crystal form B of the compound of formula (II) is shown in Table 2.
In some embodiments of the present disclosure, a DSC curve of the crystal form B described above has endothermic peaks starting points at 65.1° C.±3° C., 113.7° C.±3° C., 208.8° C.±3° C. and 221.1° C.±3° C.
In some embodiments of the present disclosure, a DSC profile of the crystal form B described above is shown in
In some embodiments of the present disclosure, a TGA curve of the crystal form B described above shows a weight loss of 3.58±0.2% at 150.0° C.±3° C.
In some embodiments of the present disclosure, a TGA profile of the crystal form B described above is shown in
In another aspect, the present disclosure provides crystal form C of the compound of formula (II), an X-ray powder diffraction pattern of which comprises characteristic peaks at 2θ values of 7.40±0.20°, 11.21±0.20° and 22.18±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form C described above using Cu Kα radiation has diffraction peaks at 2θ values of 7.40±0.20°, 11.21±0.20° and 22.18±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form C described above comprises characteristic peaks at 2θ values of 7.40±0.20°, 11.21±0.20°, 13.95±0.20°, 15.01±0.20°, 15.72±0.20°, 20.61±0.20°, 22.18±0.20° and 23.82±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form C described above comprises characteristic peaks at 2θ values of 7.40°±0.20°, 10.50°±0.20°, 11.21°±0.20°, 11.81°±0.20°, 13.05°±0.20°, 13.95°±0.20°, 15.01°±0.20°, 15.72°±0.20°, 16.28°±0.20°, 17.64°±0.20°, 18.35°±0.20°, 18.73°±0.20°, 19.53°±0.20°, 20.14°±0.20°, 20.61°±0.20°, 22.18°±0.20°, 22.51°±0.20°, 23.82°±0.20°, 24.37°±0.20°, 25.49°±0.20°, 26.36°±0.20°, 27.19°±0.20°, 28.93°±0.20°, 30.70°±0.20°, 31.60°±0.20°, 32.50°±0.20° and 34.33°±0.20°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form C described above comprises characteristic peaks at 2θ values of 7.40°, 10.50°, 11.21°, 11.81°, 13.05°, 13.95°, 15.01°, 15.72°, 16.28°, 17.64°, 18.35°, 18.73°, 19.53°, 20.14°, 20.61°, 22.18°, 22.51°, 23.82°, 24.37°, 25.49°, 26.36°, 27.19°, 28.93°, 30.70°, 31.60°, 32.50° and 34.33°.
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of the crystal form C described above is shown in
In some embodiments of the present disclosure, an X-ray powder diffraction pattern of crystal form C of the compound of formula (II) is shown in Table 3.
In some embodiments of the present disclosure, a DSC curve of the crystal form C described above has an endothermic peak starting point at 219.9° C.±3° C.
In some embodiments of the present disclosure, a DSC profile of the crystal form C described above is shown in
In some embodiments of the present disclosure, a TGA curve of the crystal form C described above shows a weight loss of 0.72±0.2% at 170.0° C.±3° C.
In some embodiments of the present disclosure, a TGA profile of the crystal form C described above is shown in
In some embodiments of the present disclosure, the X-ray powder diffraction pattern is an X-ray powder diffraction pattern of Cu Kα radiation.
In the present disclosure, the compound of formula (I) has the structure shown below:
In yet another aspect, the present disclosure provides a method for preparing a crystal form of the compound of formula (II) described above, the method comprising the following steps:
In some embodiments of the present disclosure, the method for preparing a crystal form of the compound of formula (II) described above is a method for preparing the crystal form A described above, the method comprising the following steps:
In some embodiments of the present disclosure, the method for preparing a crystal form of the compound of formula (II) described above is a method for preparing the crystal form B described above, the method comprising the following steps: mixing the crystal form A described above with acetonitrile and water, and after the solid is dissolved, volatilizing the solvents completely at 40° C. to obtain the crystal form B described above.
In some embodiments of the present disclosure, the method for preparing a crystal form of the compound of formula (II) described above is a method for preparing the crystal form C described above, the method comprising the following steps:
In yet another aspect, the present disclosure provides a crystalline composition comprising crystal form A of the compound of formula (II), crystal form B of the compound of formula (II) or crystal form C of the compound of formula (II), wherein crystal form A of the compound of formula (II), crystal form B of the compound of formula (II) or crystal form C of the compound of formula (II) makes up 50% or more, preferably 75% or more, more preferably 90% or more, and most preferably 95% or more by weight of the crystalline composition. The crystalline composition may also contain a small amount of the compound of formula (II) in other crystal forms or amorphous form.
In yet another aspect, the present disclosure provides a pharmaceutical composition comprising the compound described above or the crystal form described above, and optionally a pharmaceutically acceptable excipient.
In another aspect, the present disclosure also provides use of the compound described above or the crystal form described above or the pharmaceutical composition described above for preparing a Cdc7 inhibitor.
The present disclosure also provides use of the compound described above or the crystal form described above or the crystalline composition described above or the pharmaceutical composition described above for preparing a medicament for preventing or treating a Cdc7 kinase-mediated disease (e.g., a tumor).
The present disclosure also provides a method for preventing or treating a Cdc7 kinase-mediated disease comprising administering to a mammal, preferably a human, in need thereof a therapeutically effective amount of the compound described above or the crystal form described above or the crystalline composition described above or the pharmaceutical composition described above.
The present disclosure also provides use of the compound described above or the crystal form described above or the crystalline composition described above or the pharmaceutical composition described above in preventing or treating a Cdc7 kinase-mediated disease.
The present disclosure also provides the compound described above or the crystal form described above or the crystalline composition described above or the pharmaceutical composition described above for use in preventing or treating a Cdc7 kinase-mediated disease.
The present disclosure also provides the compound described above or the crystal form described above or the crystalline composition described above or the pharmaceutical composition described above for use as a Cdc7 inhibitor.
The present disclosure also provides the compound described above or the crystal form described above or the crystalline composition described above or the pharmaceutical composition described above for use as a medicament for preventing or treating a Cdc7 kinase-mediated disease.
In some embodiments of the present disclosure, the Cdc7 kinase-mediated disease is a tumor, e.g., colorectal cancer or pancreatic cancer.
In some embodiments of the present disclosure, the Cdc7 inhibitor is a medicament for treating a tumor, e.g., a medicament for treating colorectal cancer or pancreatic cancer.
The processes of preparing the compound and the crystal forms disclosed herein are simple, and the crystal forms are relatively stable and less affected by heat and humidity, which favors the preparation of preparations. The compound and the crystal forms thereof disclosed herein have good inhibitory activity against Cdc7: they have relatively strong inhibitory effects on enzyme CDC7/DBF4 and have inhibitory effects on the proliferation of colorectal cell COLO205 that highly expresses Cdc7. Therefore, the compound and the crystal forms disclosed herein are effective novel drugs having the potential for use in clinically treating tumors.
The crystal forms disclosed herein have advantages in such aspects as pharmaceutical activity, hygroscopicity, stability, purity, and easiness of preparation, so they can meet the requirements of the production, storage, transport, preparation, etc. of drugs.
Unless otherwise stated, the following terms and phrases used herein are intended to have the following meanings. A particular phrase or term, unless otherwise specifically defined, should not be considered as uncertain or unclear, but construed according to its common meaning. When referring to a trade name, it is intended to refer to its corresponding commercial product or its active ingredient.
The word “comprise”, and variants thereof such as “comprises” or “comprising”, or equivalents shall be understood in an open, non-exclusive sense, i.e., “includes but is not limited to”, indicating that in addition to the listed elements, components and procedures, other unspecified elements, components and procedures may also be encompassed.
The compound disclosed herein may demonstrate a specific geometric isomerism or stereoisomerism. All such compounds are contemplated herein, including cis and trans isomers, (−)- and (+)-enantiomers, (R)- and (S)-enantiomers, diastereoisomers, (D)-isomers, (L)-isomers, and racemic mixtures and other mixtures thereof, such as an enantiomer or diastereomer enriched mixture, all of which are encompassed within the scope of the present invention. Substituents such as alkyl may have an additional asymmetric carbon atom. All these isomers and mixtures thereof are encompassed within the scope of the present invention.
Unless otherwise stated, “(D)” or “(+)” stands for dextrorotation, “(L)” or “(−)” stands for levorotation, and “(DL)” or “(±)” stands for racemization.
Unless otherwise stated, the absolute configuration of a stereogenic center is represented by a wedged solid bond () and a wedged dashed bond (
), and the relative configuration of a stereogenic center is represented by a straight solid bond (
), and a straight dashed bond (
). A wavy line (
) represents a wedged solid bond (
) or a wedged dashed bond (
), or a wavy line (
) represents a straight solid bond (
) and a straight dashed bond (
).
The term “pharmaceutically acceptable” is used herein for those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problems or complications, and commensurate with a reasonable benefit/risk ratio. The term “pharmaceutically acceptable excipient” refers to an inert substance administered with an active ingredient to facilitate administration of the active ingredient, including but not limited to, any glidant, sweetener, diluent, preservative, dye/coloring agent, flavor enhancer, surfactant, wetting agent, dispersant, disintegrant, suspending agent, stabilizer, isotonizing agent, solvent or emulsifier acceptable for use in humans or animals (e.g., domesticated animals) as permitted by the National Medical Products Administration, PRC. Non-limiting examples of the excipients include calcium carbonate, calcium phosphate, various sugars and types of starch, cellulose derivatives, gelatin, vegetable oils, and polyethylene glycols.
The term “pharmaceutical composition” refers to a mixture consisting of one or more of the compounds or the crystal forms thereof disclosed herein and a pharmaceutically acceptable excipient. The pharmaceutical composition is intended to facilitate the administration of the compound disclosed herein to an organism.
The pharmaceutical composition disclosed herein can be prepared by combining the compound or the crystal forms thereof disclosed herein with a suitable pharmaceutically acceptable excipient, and can be formulated, for example, into a solid, semisolid, liquid, or gaseous formulation such as tablet, pill, capsule, powder, granule, ointment, emulsion, suspension, suppository, injection, inhalant, gel, microsphere and aerosol.
Typical routes of administration of the compound or the crystal forms thereof or the pharmaceutical composition thereof disclosed herein include, but are not limited to, oral, rectal, topical, inhalation, parenteral, sublingual, intravaginal, intranasal, intraocular, intraperitoneal, intramuscular, subcutaneous and intravenous administration.
The pharmaceutical composition disclosed herein can be manufactured using methods well known in the art, such as conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, and lyophilizing.
In some embodiments, the pharmaceutical composition is in an oral form. For oral administration, the pharmaceutical composition can be formulated by mixing the active compounds with pharmaceutically acceptable excipients well known in the art. These excipients enable the compound disclosed herein to be formulated into tablets, pills, pastilles, dragees, capsules, liquids, gels, slurries, suspensions, etc. for oral administration to a patient.
Therapeutic dosages of the compound or the crystal forms thereof disclosed herein may be determined by, for example, the specific use of a treatment, the route of administration of the compound, the health and condition of a patient, and the judgment of a prescribing physician. The proportion or concentration of the compound or the crystal form disclosed herein in the pharmaceutical composition may vary depending on a variety of factors including dosages, chemical properties (e.g., hydrophobicity), and routes of administration.
The term “treating” refers to administering the compound or the crystal form thereof or the pharmaceutical composition thereof disclosed herein to ameliorate or eliminate a disease or one or more symptoms associated with the disease, and includes:
The term “prevent”, “preventing” or “prevention” refers to administering the compound or the crystal form thereof or the pharmaceutical composition thereof disclosed herein so as to prevent a disease or one or more symptoms associated with the disease, and includes: preventing the occurrence of the disease or disease state in a mammal, particularly when such a mammal is predisposed to the disease state but has not yet been diagnosed as having it.
“Mammal” includes humans, domestic animals such as laboratory mammals and domestic pets (e.g., cat, dog, pig, cow, sheep, goat, horse, rabbit), and non-domesticated mammals such as wild mammals.
For drugs and pharmacologically active agents, the term “therapeutically effective amount” refers to an amount of a drug or a medicament that is sufficient to provide the desired effect and is non-toxic. The determination of the effective amount varies from person to person. It depends on the age and general condition of a subject, as well as the particular active substance used. The appropriate effective amount in a case may be determined by those skilled in the art in the light of conventional tests.
Unless otherwise specified clearly herein, singular terms encompass plural terms, and vice versa.
Unless otherwise stated herein, parameter values (including 2θ values and reaction conditions) are to be construed as modified by the term “about” to reflect the measurement error and the like existing in the values, e.g., there is an error of ±5% relative to the given value.
All patents, patent applications and other identified publications are explicitly incorporated herein by reference for the purpose of description and disclosure. These publications are provided solely because they were disclosed prior to the filing date of the present disclosure. All statements as to the dates of these documents or description as to the contents of these documents are based on the information available to the applicant and do not constitute any admission as to the correctness of the dates or the content of these documents. Moreover, in any country or region, any reference to these publications herein is not to be construed as an admission that the publications form part of the commonly recognized knowledge in the art.
The intermediate compounds of the present disclosure can be prepared using a variety of synthetic methods well known to those skilled in the art, including the specific embodiments listed below, embodiments formed by combinations thereof with other chemical synthetic methods, and equivalents thereof known to those skilled in the art. The preferred embodiments include, but are not limited to, the examples of the present disclosure.
The compound disclosed herein may be structurally confirmed by conventional methods well known to those skilled in the art; if the present disclosure relates to the absolute configuration of the compound, this absolute configuration may be confirmed by means of conventional techniques in the art. For example, in the single crystal X-ray diffraction (SXRD) method, intensity data of diffraction of the single crystal grown are collected with a Bruker D8 venture diffractometer, the light source is CuKα radiation, and the scan mode is φ/scan; after related data are collected, the direct method (Shelxs97) is further employed to analyze the crystal structure, and thus the absolute configuration can be confirmed.
The chemical reactions of the embodiments of the present disclosure are conducted in a proper solvent that must be suitable for the chemical changes in the present disclosure and the reagents and materials required. In order to acquire the compound disclosed herein, it is sometimes necessary for those skilled in the art to modify or select a synthesis procedure or a reaction process based on the existing embodiments.
The present disclosure is described in detail below by way of examples, which are not intended to limit the present disclosure in any way.
All the solvents used in the present disclosure are commercially available and can be used without further purification.
The solvents used in the present disclosure are commercially available.
The present disclosure uses the following abbreviations: DMF-DMA: N,N-dimethylformamide dimethyl acetal; DHP: 3,4-dihydropyran; DMF: N,N-dimethylformamide; MeOH: methanol; THF: tetrahydrofuran.
It should be noted that in an X-ray powder diffraction pattern, the position and relative intensity of a peak may vary depending on the measuring instrument, the measuring method/conditions, and other factors. For any particular crystal form, the position of a peak may have an error, and the measurement of 2θ may have an error of ±0.2°. Therefore, this error should be considered when determining each crystal form, and crystal forms within this margin of error are within the scope of the present disclosure.
It should be noted that, for the same crystal form, the position of an endothermic peak in the DSC (differential scanning calorimetry) pattern may vary depending on the measuring instrument, the measuring method/conditions, and other factors. For any particular crystal form, the position of an endothermic peak may have an error of ±5° C. or ±3° C. Therefore, this error should be considered when determining each crystal form, and crystal forms within this margin of error are within the scope of the present disclosure.
It should be noted that, for the same crystal form, the temperature and value of a weight loss in the TGA pattern may vary depending on the measuring instrument, the measuring method/conditions, and other factors. For any particular crystal form, the temperature and value of a weight loss may have errors: the weight loss temperature may have an error of ±5° C. and the weight loss value may have an error of ±0.2%. Therefore, this error should be considered when determining each crystal form, and crystal forms within this margin of error are within the scope of the present disclosure.
It should be noted that in an infrared spectrum, the position of a peak may vary depending on the measuring instrument, the measuring method/conditions, and other factors. For any particular crystal form, the position of a peak may have an error: the position of a peak may have a measurement error of ±5 cm−1. Therefore, this error should be considered when determining each crystal form, and crystal forms within this margin of error are within the scope of the present disclosure.
It should be noted that in an ultraviolet absorption spectrum, the position of a peak may vary depending on the measuring instrument, the measuring method/conditions, and other factors. For any particular crystal form, the position of a peak may have an error, and the wavelength may have a measurement error of ±5 nm or ±3 nm. Therefore, this error should be considered when determining each crystal form, and crystal forms within this margin of error are within the scope of the present disclosure.
Unless otherwise stated herein, “room temperature” in the present disclosure refers to normal temperature, typically 20-30° C.
Instrument model: X'Pert3 X-ray diffractometer from PANalytical Inc.
Method: take about 10 mg sample for XRPD analysis.
The XRPD parameters are detailed below:
X-ray tube: Cu, kα (λ=1.54060 Å)
Voltage of X-ray tube: 45 kV, current of X-ray tube: 40 mA
Divergent slit: 0.60 mm
Detector slit: 10.50 mm
Anti-scatter slit: 7.10 mm
Scan range: 3-40 deg
Step size: 0.0263 deg
Step time: 0.12 s
Rotational speed of sample tray: 15 rpm
Instrument model: TA 2500 differential scanning calorimeter
Method: take a sample (0.5-1 mg) and put it into a DSC aluminum pan for analysis: under 50 mL/min N2, heat the sample from room temperature (25° C.) to 300° C. or 350° C. at a rate of 10° C./min.
Instrument model: TA Q5500/Q5000 thermogravimetric analyzer
Method: take a sample (2-5 mg) and put it into a TGA platinum pan for analysis: under 25 mL/min N2, heat the sample from room temperature (25° C.) to 300° C. or 350° C. at a rate of 10° C./min to make a weight loss of 20%.
Instrument model: Agilent 1200 high performance liquid chromatograph
The analytical method is as follows:
The present disclosure is described in detail below by way of examples. However, this is by no means disadvantageously limiting the scope of the present disclosure. Although the present disclosure has been described in detail herein and specific embodiments have also been disclosed, it will be apparent to those skilled in the art that various changes and modifications can be made to the specific embodiments without departing from the spirit and scope of the present disclosure.
DMF-DMA (19.05 mol, 1.0 eq, 2.53 L) was added to a 30 L high-low temperature reaction kettle at room temperature, and starting material 1-A (2403.52 g, 19.05 mol, 1.0 eq) was added in portions with stirring. The reaction was slightly exothermic, so the rate of dropwise addition was controlled to ensure that the internal temperature of the reaction mixture did not exceed 35° C. After the addition, the reaction mixture was heated to 85-90° C., stirred at that temperature for 3 h, and cooled to 50-55° C. Methanol (2.4 L) was added as a solvent, and then hydrazine hydrate (17.15 mol, 98% purity, 0.9 eq, 850 mL) was slowly added dropwise. The reaction was exothermic, so the rate of addition was controlled to prevent bumping. After the dropwise addition, the mixture was reacted at 50-60° C. for 0.5 h. The reaction mixture was cooled to room temperature and concentrated at 50° C. under a reduced pressure of −0.08 MPa. The resulting solid was triturated with ethyl acetate (2.4 L). After 0.5 h of stirring, the mixture was filtered, and the filter cake was collected. The filtrate was concentrated under reduced pressure to give a crude product. The crude product was triturated with ethyl acetate (1.2 L). After 0.5 h of stirring, the mixture was filtered, and the filter cake was collected. The filter cakes were combined and dried in vacuo at 45-50° C. under−0.08 MPa to constant weight to give 1-B. LCMS (ESI) m/z: 151 (M+1); 1H NMR (DMSO-d6, 400 MHz) δ ppm 13.02 (s, 1H), 7.82 (s, 1H), 2.91-2.96 (m, 2H), 2.56-2.61 (m, 2H), 1.85-1.91 (m, 2H), 1.78-1.84 (m, 2H).
Dichloromethane (11.07 L) was added as a solvent to a 50 L high-low temperature reaction kettle at room temperature, and intermediate 1-B (2214.90 g, 14.74 mol, 1.0 eq) and N,N-dimethylformamide (3.40 L, 44.23 mol, 3.0 eq) were added in portions with stirring. Oxalyl chloride (3.87 L, 44.23 mol, 3.0 eq) was then slowly added dropwise, and the rate of addition was controlled so the internal temperature of the reaction mixture did not exceed 30° C. The reaction mixture was stirred at 20-30° C. for 12 h and concentrated to dryness at 40° C. under a reduced pressure of −0.08 MPa. The resulting crude product was added in portions to ice water (22.14 L) with stirring at room temperature. After 0.5 h of stirring, the mixture was filtered, and the filter cake was collected, washed with water (2 L×3), and dried in vacuo at 35-40° C. under −0.08 MPa to give 1-C. LCMS (ESI) m/z: 197 (M+1); 1H NMR (DMSO-d6, 400 MHz) δ ppm 10.2 (s, 1H), 8.03 (s, 1H), 2.9-3.0 (m, 2H), 2.6-2.6 (m, 2H), 1.8-1.8 (m, 2H).
THF (14.65 L) was added as a solvent to a 50 L high-low temperature reaction kettle at room temperature, and intermediate 1-C (2929.34 g, 14.84 mol, 1.0 eq) and concentrated ammonium hydroxide (4.57 L, 29.69 mol, 2.0 eq) were added in portions with stirring. Elemental iodine (7540.23 g, 29.69 mol, 2.0 eq) was slowly added in portions, and the rate of addition was controlled so the internal temperature of the reaction mixture did not exceed 35° C. The reaction mixture was stirred at 20-35° C. for 12 h and added to 120 L of saturated aqueous sodium sulfite solution with stirring. After 0.5 h of stirring, the mixture was filtered, and the filter cake was collected, washed with water (3 L×3), and dried in vacuo at 45-50° C. under −0.08 MPa to constant weight to give 1-D. LCMS (ESI) m/z: 194 (M+1); 1H NMR (DMSO-d6, 400 MHz) δ ppm 13.06-13.27 (m, 1H), 7.71-8.11 (m, 1H), 2.90-2.96 (m, 2H), 2.67 (m, 2H), 1.90 (m, 2H).
THF (9.70 L) was added to a 50 L high-low temperature reaction kettle at room temperature, and intermediate 1-D (1.94 kg, 9.82 mol, 98.061% purity, 1 eq) and DHP (2.48 kg, 29.47 mol, 2.69 L, 3.0 eq, 1000 mL/min) were then added in sequence to the reaction kettle. The reaction mixture was stirred at 75-80° C. for 65 h and concentrated at 50° C. under a reduced pressure of −0.08 MPa to give an oil. DMF (15.90 L) was added as a solvent to a 50 L high-low temperature reaction kettle at room temperature, and the oil obtained above (3180.26 g, 11.45 mol, 1 eq), mercaptoacetamide (1252.14 g, 13.74 mol, 1.2 eq) and potassium carbonate (3323.28 kg, 24.05 mol, 2.1 eq) were sequentially added in portions to the reaction kettle as starting materials. The reaction was not exothermic. The mixture was reacted at 75-80° C. for 1 h (the passages of the system were kept clear, a small flow of nitrogen was maintained and constant stirring was ensured; gaseous waste should be absorbed with a sodium hypochlorite solution, and a buffer safety bottle was arranged between the gaseous waste absorption setup and the reaction kettle) and cooled to room temperature, and ethyl acetate (15.90 L) was added. The reaction mixture was slowly poured into 160 L of water with stirring. After the addition, a suspension was obtained. The suspension was stirred at room temperature for 12 h and then filtered (solid precipitation was slow), and the filter cake was collected, washed with water (3 L×3), and added to 15.90 L of methanol. The mixture was stirred at 65-70° C. for 1 h, cooled to room temperature, and then filtered, and the filter cake was collected (if it was not pure enough, the above purification process may be repeated) and dried in vacuo at 45-50° C. under −0.08 MPa to give 1-E. LCMS (ESI) m/z: 333 (M+1); 1H NMR (DMSO-d6, 400 MHz) δ ppm 7.52-7.92 (m, 1H), 6.62-6.89 (m, 2H), 6.29-6.50 (m, 2H), 5.22-5.52 (m, 1H), 3.83-3.99 (m, 1H), 3.55-3.70 (m, 1H), 2.84-3.18 (m, 2H), 2.60-2.71 (m, 2H), 1.86-2.35 (m, 5H), 1.43-1.75 (m, 3H).
Starting material 1-F (906.50 g, 4.73 mol, 1 eq, HCl) was added to dichloromethane (9.6 L) at room temperature, and oxalyl chloride (331.13 g, 2.61 mol, 228.37 mL, 5 eq) was added dropwise at 10-15° C. The mixture was then stirred for 72 h and concentrated to dryness under reduced pressure. 5 L of DCM was added and the mixture was concentrated under reduced pressure to remove the residual oxalyl chloride; the process was repeated once. The resulting 1-G was directly used in the next step.
9.2 L of dichloromethane was added to a 30 L high-low temperature reaction kettle at room temperature, and 1-G (983.28 g, 4.68 mol, 1.7 eq, HCl) was added to the reaction kettle with stirring. The mixture was cooled to −20 to −15° C. 1-E (920.19 g, 2.77 mol, 1 eq) was added in portions to the reaction kettle, and the internal temperature was controlled at below −10° C. The mixture was stirred at −20 to −10° C. for 0.5 h. HPLC analysis showed the 1-E content <5%. Triethylamine (192 mL, 1.39 mol, 0.5 eq) was added dropwise to the reaction kettle at −20 to −10° C. A sample was then taken, and HPLC analysis showed the 1-E content <1%. The remaining triethylamine (960 mL, 6.93 mol, 2.5 eq) was added dropwise to the reaction kettle. The mixture was concentrated under reduced pressure, and the resulting solid (about 3 kg) was triturated with 15 L of n-heptane to remove dichloromethane. The mixture was filtered, and the solid was collected and dried under reduced pressure (45° C., −0.1 MPa) to give 1-H-M.
8.7 L of methanol and 4.3 L of water (methanol:water=2:1) were added to a high-low temperature reaction kettle, and after sodium hydroxide (1107.27 g, 27.7 mol, 10 eq) was added, the temperature was raised to 50° C. The dried 1-H-M was added in portions to the reaction kettle. After the mixture was stirred for 0.5 h, HPLC analysis showed the reaction was complete. The mixture was cooled to 0° C., and 13 L of water was added. The mixture was stirred at 0-5° C. for 0.5 h and filtered, and the solid was collected. The filtrate was adjusted to pH 7-8 with 6 N hydrochloric acid and concentrated under reduced pressure to remove most methanol. The pH was adjusted to 10-11 with 2 N sodium hydroxide at 10-15° C., and a large amount of solid precipitated. The mixture was stirred for another 0.5 h and filtered, and the filter cake was collected. The filter cakes were combined and washed with water (4 L×2). After filtration, the filter cake was dried under reduced pressure (55° C. and −0.08 MPa) to give 1-H. LCMS (ESI) m/z: 452 (M+1); 1H NMR (DMSO-d6+D2O, 400 MHz) δ ppm 7.66-8.08 (m, 1H), 5.30-5.43 (m, 1H), 3.85-4.00 (m, 1H), 3.55-3.76 (m, 3H), 2.89-3.12 (m, 6H), 2.74-2.87 (m, 1H), 2.37-2.45 (m, 1H), 2.05-2.22 (m, 1H), 1.86-2.05 (m, 4H), 1.76-1.84 (m, 1H), 1.61-1.75 (m, 2H), 1.60 (br s, 5H), 1.28-1.41 (m, 1H).
1-H (1197.21 g, 2.65 mol, 1 eq) was added in portions to a 4 N solution of hydrogen chloride in ethyl acetate (11.97 L, 47.88 mol, 18.1 eq) with stirring at 20-25° C. After the addition, the mixture was stirred at 10-15° C. for 0.5 h and then filtered, and the filter cake was washed with ethyl acetate (1 L×2) and dried under reduced pressure (40-45° C., −0.1 MPa) for 16 h to give 1-I-M. 1-I-M was added to a mixed solvent of water (9.6 L) and methanol (1.2 L), and the pH was adjusted to 8-9 with concentrated ammonium hydroxide. The mixture was stirred for 0.5 h and filtered, and the filter cake was collected and washed with water (5 L×2). After filtration, the filter cake was collected and dried under reduced pressure (55° C., −0.1 MPa) to give 1-I. LCMS (ESI) m/z: 368 (M+1); 1H NMR (DMSO-d6+D2O, 400 MHz) δ ppm 7.46 (s, 1H), 3.64-3.78 (m, 1H), 2.89-3.03 (m, 5H), 2.72-2.89 (m, 3H), 2.30-2.36 (m, 1H), 1.73-1.88 (m, 4H), 1.35-1.54 (m, 4H).
L of methanol was added to a 30 L high-low temperature reaction kettle at room temperature, and 1-I (807.89 g, 2.20 mol, 1 eq) was added to the reaction kettle. Glacial acetic acid (4 L) and water (404 mL) were added in sequence with stirring. When the temperature of the reactor reached 60° C., 3.7 L of methanol in which (+)-di-p-toluoyl-D-tartaric acid (374.59 g, 0.97 mmol, 0.44 eq) was dissolved was added in portions to the reactor. The mixture was stirred at 60-65° C. for 12 h, cooled to 20° C. at a rate of 10° C./30 min, and then filtered, and the filter cake was washed with methanol (4.6 L). After filtration, the filter cake was dispersed in a mixed solvent of water (8 L) and methanol (1 L). The mixture was adjusted to pH 8-9 with concentrated ammonium hydroxide, stirred for 0.5 h, and filtered. The filter cake was collected and washed twice with water by trituration. After filtration, the filter cake was collected and dried under reduced pressure (55° C., −0.1 MPa) to give 1-J-M1 (89.116% ee).
3.78 L of methanol and 1-J-M1 (302.67 g, 0.824 mol, 1 eq) were added to a 10 L high-low temperature reaction kettle at room temperature. Glacial acetic acid (1.5 L) and water (151 mL) were added with stirring. When the temperature of the reactor reached 60° C., 2.74 L of methanol in which (+)-di-p-toluoyl-D-tartaric acid (273.67 g, 0.708 mmol, 0.86 eq) was dissolved was added in one portion to the reactor. The reaction mixture was stirred at 60-65° C. for 12 h under a small flow of nitrogen, cooled to room temperature at a rate of 10° C./30 min, and then filtered, and the filter cake was triturated with methanol (3.2 L). After filtration, the filter cake was dispersed in a mixed solvent of water (8.52 L) and methanol (1.07 L). The mixture was adjusted to pH 8-9 with concentrated ammonium hydroxide, stirred for 0.5 h, and then filtered. The filter cake was collected and washed twice with water. After filtration, the filter cake was collected and dried under reduced pressure (55° C., −0.1 MPa) to give 1-J-M2 (98.040% ee).
3.14 L of methanol was added to a 10 L high-low temperature reaction kettle at room temperature, and 1-J-M2 (251.36 g, 0.68 mol, 1 eq) was added to the reaction kettle. Glacial acetic acid (1.25 L) and water (125 mL) were added in sequence with stirring. When the temperature of the reactor reached 60° C., 2.39 L of methanol in which (+)-di-p-toluoyl-D-tartaric acid (239.22 g, 0.62 mmol, 0.91 eq) was dissolved was added in one portion to the reactor. The reaction mixture was stirred at 60-65° C. for 12 h under a small flow of nitrogen, cooled to room temperature at a rate of 10° C./30 min, and then filtered, and the filter cake was triturated with methanol (3 L). After filtration, the filter cake was dispersed in a mixed solvent of water (4.8 L) and methanol (0.6 L). The mixture was adjusted to pH 8-9 with concentrated ammonium hydroxide, stirred for 0.5 h, and filtered. The filter cake was collected and washed twice with water by trituration. After filtration, the filter cake was collected and dried under reduced pressure (55° C., −0.1 MPa) to give the compound of formula (I) (99.76% ee). LCMS (ESI) m/z: 368 (M+1); 1H NMR (DMSO-d6, 400 MHz): δ (ppm) 7.92 (m, 1H), 3.86-3.92 (m, 1H), 3.03-3.13 (m, 5H), 2.82-2.92 (m, 1H), 2.55-2.67 (m, 2H), 2.29-2.39 (m, 1H), 1.93-2.05 (m, 2H), 1.83-1.92 (m, 1H), 1.70-1.81 (m, 1H), 1.50-1.62 (m, 2H), 1.37-1.49 (m, 2H).
Methanol (1042 mL) was added to a 3 L reaction flask at room temperature, and the compound of formula (I) (45.00 g, 122.46 mmol, 1 eq) was then added to the reaction flask. The mixture was cooled to 5° C. with stirring, and maleic acid (21.32 g, 183.69 mol, 1.5 eq) was added thereto. The mixture was stirred at 5-13° C. for 6 h and filtered, and the filter cake was collected, washed with methanol (500 mL×2), and then dried under reduced pressure (45° C., −0.1 MPa) to give crystal form A of the compound of formula (II). LCMS (ESI) m/z: 368 (M+1); 1H NMR (heavy water, 400 MHz): δ ppm 7.46 (s, 1H), 6.16 (s, 2H), 4.45-4.55 (m, 1H), 3.64-3.81 (m, 1H), 3.48-3.59 (m, 1H), 3.34-3.47 (m, 2H), 2.76-2.87 (m, 2H), 2.65-2.76 (m, 2H), 2.41-2.53 (m, 1H), 2.26-2.37 (m, 1H), 1.95-2.09 (m, 3H), 1.85-1.95 (m, 2H), 1.63-1.84 (m, 2H).
1H NMR analysis shows that the salt formation number of crystal form A of the compound of formula (II) is 1.
Crystal form A of the compound of formula (II) (60.91 mg) was measured out and added to a mixture of acetonitrile (1 mL) and water (1 mL), and the solid was dissolved by stirring. The solvent was completely volatilized at 40° C., and a solid precipitated. The solid was collected and dried under reduced pressure (55° C., −0.1 MPa) to give crystal form B of the compound of formula (II). LCMS (ESI) m/z: 368 (M+1); 1H NMR (DMSO-d6+D2O, 400 MHz): δ ppm 7.85 (s, 1H), 6.10 (s, 2H), 4.60-4.64 (m, 1H), 3.74-3.86 (m, 1H), 3.38-3.52 (m, 1H), 3.21-3.37 (m, 2H), 3.04 (br s, 4H), 2.37-2.47 (m, 1H), 2.16-2.24 (m, 1H), 2.02-2.12 (m, 1H), 1.91-2.01 (m, 2H), 1.87 (br s, 4H).
1H NMR analysis shows that the salt formation number of crystal form B of the compound of formula (II) is 1.
Methanol (56 mL) was added to the compound of formula (I) (0.80137 g, 2.18 mmol, 1 eq) at room temperature, and maleic acid (505.29 mg, 4.35 mmol, 2 eq) was added with stirring at 75° C. The mixture was stirred at 75° C. for 0.5 h and at 40° C. for 1 h, cooled to 25° C., stirred for another 2 h, and filtered. The solid was collected, washed with methanol (10 mL×2), and dried under reduced pressure (55° C., −0.1 MPa) to give crystal form C of the compound of formula (II). LCMS (ESI) m/z: 368 (M+1); 1H NMR (DMSO-d6+D2O, 400 MHz): δ ppm 7.92 (s, 1H), 6.09 (s, 2H), 4.62-4.64 (m, 1H), 3.62-3.75 (m, 1H), 3.39-3.54 (m, 1H), 3.23-3.37 (m, 2H), 3.04 (br s, 4H), 2.37-2.48 (m, 1H), 2.15-2.28 (m, 1H), 2.02-2.14 (m, 1H), 1.92-2.01 (m, 2H), 1.87 (br s, 4H).
1H NMR analysis shows that the salt formation number of crystal form C of the compound of formula (II) is 1.
50 mg of crystal form A of the compound of formula (II) was measured and placed in a dry, clean glass bottle, spread into a thin layer, and left to stand under accelerated conditions (60° C., 25/92.5% RH, 40° C./75% RH and 60° C./75% RH). The sample was completely exposed, and the bottle was covered with aluminum foil in which holes are pricked. Samples were taken at 5 days, 10 days, 1 month, 2 months and 3 months and analyzed. The results are shown in Table 6.
Conclusion: the impurity content of crystal form A of the compound of formula (II) substantially did not change under high temperature and high humidity conditions—the stability is good.
A dry glass weighing bottle with a lid was placed in a dryer with saturated ammonium chloride solution placed in the lower part, and the weighing bottle was left to stand uncovered. A lid was put on the dryer, and the dryer was placed in a 25° C. box and left to stand overnight. After being left to stand overnight, the weighing bottle was taken out and precisely weighed, and the weight was denoted by m1.
A proper amount of crystal form A of the compound of formula (II) was measured out and spread in the weighed measuring bottle (the sample was about 1 mm thick). The measuring bottled was then precisely weighed, and the weight was denoted by m2.
The weighing bottle, together with its lid, was placed in a dryer with saturated ammonium chloride solution placed in the lower part and left to stand uncovered. A lid was put on the dryer, and the dryer was placed in a 25° C. box and left to stand for 24 h. After 24 h of sitting, the lid was put on the weighing bottle and the bottle was taken out and precisely weighed, the weight denoted by m3.
The hygroscopic weight gain was calculated using the formula: percent weight gain=100%×(m3−m2)/(m2−m1).
Experimental results: crystal form A of the compound of formula (II) made a weight gain of 0.18% (mean).
Conclusion: the mean hygroscopicity value of crystal form A of the compound of formula (II) is less than 0.2%, so crystal form A of the compound of formula (II) is non-hygroscopic or hardly hygroscopic.
Instrument: Thermo Fisher Nicolet iS5
Method: attenuated total reflection (ATR)
Experimental results: the infrared absorption spectroscopic results of crystal form A of the compound of formula (II) are shown in Table 7. The infrared absorption spectrum of crystal form A of the compound of formula (II) is shown in
Conclusion: the structure of the test compound contains such groups as —NH, —OH, benzene ring, —CH2, —CH3, C—O, —C═O and —C═N, agreeing with the structure of crystal form A of the compound of formula (II).
Instrument: Agilent G6860A ultraviolet-visible spectrophotometer
Experimental results: the ultraviolet results of crystal form A of the compound of formula (II) are shown in Table 8. The ultraviolet absorption spectrum of crystal form A of the compound of formula (II) is shown in
Analytical conclusion: the test compound has ultraviolet absorptions in water at λ=259.0 nm, λ=214.4 nm and λ=322.6 nm, which are B band and R band absorption peaks of an aromatic ring, indicating that the structure contains an aromatic ring, agreeing with the structure of crystal form A of the compound of formula (II).
CDC7 kinase plays an important role in the initiation of DNA replication and DNA damage response. When CDC7 is abnormally highly expressed, the abnormal activation of the CDC7-MCM2 signal pathway axis will cause abnormal replication of DNA, and the activity of CHK2 and MK2, which are key checkpoint proteins for DNA damage repair, are inhibited, such that apoptosis is inhibited and proliferation is promoted, leading to tumorigenesis.
The ADP-Glo™ kinase assay is a chemiluminescent kinase assay. The reaction of kinase with substrate requires ATP to provide energy, thereby converting ATP into ADP. The enzymatic activity is reflected by the amount of ADP produced in the reaction. In the assay, ADP is converted into ATP, and then ATP binds to Ultra-Glo™ Luciferase with light emitted; the intensity of light emission is directly proportional to the amount of ADP.
The ADP-Glo™ assay was used to investigate the inhibitory effect of crystal form A of the test compound of formula (II) on the activity of CDC7/DBF4 kinase. The highest concentration of the test compound was 10 μM, and 5-fold serial dilution was performed to obtain 8 concentration points. The compound was re-tested on a different day, and each test was performed in duplicate.
The kit contains an ADP-Glo™ reagent, a kinase assay solution, a kinase assay solution matrix and a 10 mM ATP stock solution.
Preparation of the kinase assay solution: The kinase assay buffer and the kinase assay solution matrix were equilibrated to room temperature and then mixed. After complete dissolution, the solution was ready. The unused solution should be aliquoted and stored at −20° C.
ADP-Glo™ reagent: The reagent was equilibrated to room temperature at the time of the first use. The unused solution should be aliquoted and stored at −20° C.
% enzymatic activity=[(RLU(compound)−RLU(blank))/(RLU(positive)−RLU(blank))]×100%
The percent enzymatic activity was calculated from the original readings according to the calculation formula. A graph was then plotted using Prism and the IC50 of the compound was calculated. The results are shown in the table below.
Conclusion: crystal form A of the compound of formula (II) has a relatively strong inhibitory effect on enzyme CDC7/DBF4.
In the assay, the inhibitory effect of crystal form A of the compound of formula (II) on the proliferation of colorectal cell COLO205 that highly expresses Cdc7 was investigated.
Crystal form A of the compound of formula (II).
1640 medium, Biological Industries; bispecific antibody, Procell; 0.25% pancreatin, Base Media; fetal bovine serum, Biosera; DMSO, Sinopharm; 96-well cell culture plate, Corning; 96-well compound plate, Shanghai Jingrao Biotech Ltd.; CellTiter-Glo® Luminescent Cell Viability Assay, Promega; cell culture dish, Nest.
CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous rapid assay for the viability of living cells in cultures by ATP quantification. ATP is an indicator of the metabolism of living cells. Cell lysis and the luminescence signals generated are directly proportional to the amount of ATP that exists, and the amount of ATP is directly proportional to the number of living cells in cultures.
Cells having achieved 80%-90% cell fusion were digested with pancreatin. After the digestion was stopped with 1 mL of culture medium, the cells were transferred to a new culture dish according to a proper proportion, and 10 mL of fresh cell culture medium was added to the culture dish. Then the cell culture dish was placed in a 37° C., 5% CO2 incubator to continue the culture.
The compound was diluted to 2 mM with 100% DMSO—that is, 5 μL of 10 mM compound stock solution was transferred to column 1 of a 96-well compound plate, 20 μL of 100% DMSO was then added, and the mixture was well mixed. To column 2 through column 9 of the 96-well compound plate was added 20 μL of 100% DMSO. From column 1, 10 μL of compound was transferred to column 2, and the mixture was well mixed. Then 10 μL of compound was transferred from column 2 to column 3, and the mixture was well mixed. This procedure was repeated to column 9 to complete the serial dilution of the compound. Preparation of compound working solutions: From the compound plate, 2 μL/well of compound was transferred to a new 96-well compound plate, and 78 μL/well of cell culture medium was added. To a negative control well was added 2 μL of 100% DMSO followed by 78 μL of cell culture medium. The compound concentration was the final concentration. The blank control well was a culture medium well containing no cells. The negative control was 0.5% DMSO.
COLO205 cells having achieved 80%-90% cell fusion were digested with pancreatin and counted. After counting, the cell suspension was diluted with cell culture medium to a desired density. The cell density is shown in the table below. To each well of a 96-well cell culture plate was inoculated 80 μL of cell suspension. The plate was incubated in a 37° C., 5% CO2 incubator overnight. On the day of the experiment, 20 μL of compound working solution was added to each well of the cell plate, and the plate was incubated in a 37° C., 5% CO2 incubator for another period of time. The specific cell plating density and the number of days of incubation are shown in the table below.
The plating density of COLO205 cells and the length of the co-incubation of the cells and the compound
After the incubation, 100 μL of CTG assay reagent was added to each well of the cell plate, and the plate was incubated at 25° C. for 10 min. After the incubation, luminescence signals were measured on Envision.
The signal value of the background blank control well was subtracted from the measured signal value of each group for standardization. The standardized data were used to calculate the cell viability after compound treatment according to the following formula: % inhibition=[1−(RFUcompound−RFUblank control)/(RFUpositive control−RFUblank control)]×100%. Blank control: a treatment involving no cells but culture medium only. Positive control: cells treated with 0.5% DMSO; a graph was then plotted using Prism and the IC50 value of the compound was calculated.
The percent inhibition was calculated from the original readings according to the calculation formula. A graph was then plotted using Prism and the IC50 of the compound was calculated.
The inhibitory effect IC50 of crystal form A of the compound of formula (II) on the proliferation of COLO205 cells is shown in the table below.
Conclusion: crystal form A of the compound of formula (II) has a relatively strong inhibitory effect on COLO205 cells.
The steric configuration of the compound of formula (I) was confirmed by single-crystal X-ray diffraction.
A 20-mg sample of the compound of formula (I) was measured out and dissolved in 1 mL of methanol/methyl tert-butyl ether (1:1) at room temperature, and the sample solution was placed in a 4 mL semi-sealed sample bottle to allow the solvents to be slowly volatilized at room temperature. A colorless columnar crystal was obtained on day three and its structure was analyzed by single-crystal X-ray diffraction.
Experimental results: the ellipsoid plot of the three-dimensional structure of the compound of formula (I) is shown in
| Number | Date | Country | Kind |
|---|---|---|---|
| 202011383418.7 | Nov 2020 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CN2021/134435 | 11/30/2021 | WO |
