Claims
- 1. A substantially pure SAM symthetase from Streptomyces fradiae having the amino acid sequence;
- 2. A substantially pure methyltransferase from Streptomyces fradiae having the amino acid sequence:
- 3. A substantially pure MTHR from Streptomyces fradiae having the amino acid sequence:
- 4. An isolated nucleic acid compound encoding the protein of claim 1.
- 5. An isolated nucleic acid compound comprising a sequence encoding the protein of claim 1 or fragment thereof wherein said compound has a sequence selected from the group consisting of:
(a) residues 986 through 2209 of SEQ ID NO:1; (b) residues 986 through 2209 of SEQ ID NO:6 (c) a nucleic acid compound complementary to (a) or (b); and (d) a fragment of (a), (b), or (c) that is at least 18 base pairs in length and which will selectively hybridize to genomic DNA encoding SEQ ID NO. 2.
- 6. An isolated nucleic acid compound of claim 5 wherein the sequence is (a) or a sequence complementary to (a).
- 7. An isolated nucleic acid compound of claim 5 wherein the sequence of said compound is (b) or a sequence complementary to (b).
- 8. An isolated nucleic acid compound encoding the protein of claim 2.
- 9. An isolated nucleic acid compound comprising a sequence encoding the protein of claim 2 or fragment thereof wherein said compound has a sequence selected from the group consisting of:
(a) residues 2241 through 3341 of SEQ ID NO:1; (b) residues 2241 through 3341 of SEQ ID NO:6 (c) a nucleic acid compound complementary to (a) or (b); and (d) a fragment of (a), (b), or (c) that is at least 18 base pairs in length and which will selectively hybridize to genomic DNA encoding SEQ ID NO. 3.
- 10. An isolated nucleic acid compound of claim 9 wherein the sequence is (a) or a sequence complementary to (a).
- 11. An isolated nucleic acid compound of claim 9 wherein the sequence of said compound is (b) or a sequence complementary to (b).
- 12. An isolated nucleic acid compound encoding the protein of claim 3.
- 13. An isolated nucleic acid compound comprising a sequence encoding the protein of claim 3 or fragment thereof wherein said compound has a sequence selected from the group consisting of:
(a) residues 3338 through 4255 of SEQ ID NO:4; (b) residues 3338 through 4255 of SEQ ID NO:6 (c) a nucleic acid compound complementary to (a) or (b); and (d) a fragment of (a), (b), or (c) that is at least 18 base pairs in length and which will selectively hybridize to genomic DNA encoding SEQ ID NO. 5.
- 14. An isolated nucleic acid compound of claim 13 wherein the sequence is (a) or a sequence complementary to (a).
- 15. An isolated nucleic acid compound of claim 13 wherein the sequence of said compound is (b) or a sequence complementary to (b).
- 16. An isolated nucleic acid compound encoding the SAM operon from Streptomyces fradiae , wherein said compound is SEQ ID NO.1 or SEQ ID NO.6.
- 17. A vector comprising an isolated nucleic acid compound of claim 16 wherein said compound is SEQ ID NO.1.
- 18. A vector comprising an isolated nucleic acid compound of claim 5 in operable linkage to a promoter sequence.
- 19. A vector comprising an isolated nucleic acid compound of claim 9 in operable linkage to a promoter sequence.
- 20. A vector comprising an isolated nucleic acid compound of claim 13 in operable linkage to a promoter sequence.
- 21. A host cell containing a vector of claim 17.
- 22. A host cell containing a vector of claim 18.
- 23. A host cell containing a vector of claim 19.
- 24. A host cell containing a vector of claim 20.
- 25. A method for constructing a recombinant host cell having the potential to express SEQ ID NO:2, SEQ ID NO.3, and SEQ ID NO.5 said method comprising introducing into a host cell by any suitable means a vector of claim 16.
- 26. A method as in claim 25 wherein said host cell is an Actinomycete.
- 27. A method for expressing SEQ ID NO:2, SEQ ID NO.3 and SEQ ID NO.5 in the recombinant host cell of claim 26, said method comprising culturing said recombinant host cell under conditions suitable for gene expression.
- 28. A method for synthesizing S-adenosylmethionine, comprising the steps of:
a) admixing a suitable amount of the following reagents:
i) a substantially pure SAM synthetase as claimed in claim 1, ii) ATP; iii) methionine; and b) purifying said S-adenosylmethionine by any suitable means.
- 29. A method for producing S-adenosylmethionine comprising the steps of:
a) preparing a crude extract from the recombinant cells of claim 22;b) adding to said extract suitable amount of ATP and methionine; and c) recovering said S-adenosylmethionine by any suitable means.
- 30. A method for producing S-adenosylmethionine comprising the steps of:
a) culturing the host cell of claim 21 under conditions suitable for synthesis of S-adenosylmethionine, and b) recovering S-adenosylmethionine from said host cell by any suitable means.
Parent Case Info
[0001] We hereby claim the benefit under Title 35, United States Code, §119(e) of U.S. provisional patent application No. 60/030,898 filed Nov. 13, 1996
Provisional Applications (1)
|
Number |
Date |
Country |
|
60030898 |
Nov 1996 |
US |
Divisions (1)
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Number |
Date |
Country |
Parent |
08955957 |
Oct 1997 |
US |
Child |
09943702 |
Aug 2001 |
US |