Claims
- 1. A method of genetic analysis by:
a. upstream processing a tissue sample; b. applying the processed sample on a matrix, including preserving means sorbed to the solid matrix for protecting the genetic material from degradation, to derive genetic material from the sample; and c. analyzing the genetic material.
- 2. The method as set forth in claim 1, wherein the analyzing step further includes phenotyping the processed tissue sample and cells therein.
- 3. The method as set forth in claim 1, wherein the matrix further comprises:
a. a weak base; b. a chelating agent; c. an anionic surfactant or detergent.
- 4. The method as set forth in claim 1, wherein the upstream processing step further includes dissociating the cells of the tissue sample.
- 5. A kit for genetic analysis including:
a. upstream processing means for processing a tissue sample; and b. a matrix, including preserving means sorbed to the matrix for protecting the genetic material from degradation, for receiving a processed sample.
- 6. A method of genetic analysis, wherein the method comprises:
a. upstream processing of a biological sample; b. applying the processed sample to a matrix, including preserving means sorbed to the matrix for protecting the genetic material from degradation, to derive genetic material from the sample; and c. analyzing the genetic material.
- 7. A method of genetic analysis, wherein the method comprises:
a. upstream processing of a biological sample to produce a suspension comprising cells comprising genetic material; b. applying the suspension to a first solid medium; c. contacting the cells on the first solid medium with a second solid medium comprising a matrix, including preserving means sorbed to the matrix for protecting the genetic material from degradation, to derive genetic material from the sample; and d. analyzing the genetic material.
- 8. A method of isolating and analyzing genetic material, wherein the method comprises:
a. obtaining a biological sample; b. processing the biological sample to obtain one or more cells or virions comprising genetic materal; c. applying the sample to a solid medium, wherein the solid medium comprises a matrix having a composition sorbed thereto, wherein the composition comprises:
i. a weak base; ii. a chelating agent; and iii. an anionic surfactant or detergent; d. lysing the cell or virion and retaining the genetic material with the solid medium; e. analyzing the genetic material.
- 9. The method of claim 8, wherein
a. the biological sample comprises an organ, a tissue, or a multi-cellular organism or colony; and b. the processing step b further comprises:
i. dissociating cells in the biological sample; and ii. isolating the cells on a solid medium distinct from the solid medium of step c.
- 10. The method of claim 8, wherein the genetic material comprises DNA or RNA.
- 11. A method of detecting and analyzing genetic material from a biological sample, wherein the method comprises:
a. obtaining a biological sample comprising a cellular component having one or more cells comprising genetic material; b. isolating the cellular component, on a first solid medium, from non-cellular components in the sample; c. contacting the cellular component with a second solid medium, wherein the second solid medium comprises a matrix having a composition sorbed thereto, wherein the composition comprises:
i. a weak base; ii. a chelating agent; and iii. an anionic surfactant or detergent; d. lysing the one or more cells in the cellular component and retaining the genetic material with the second solid medium; and e. analyzing the genetic material.
- 12. The method of claim 11, wherein the biological sample comprises blood, plasma, serum, mucus, urine, saliva, sweat, or semen.
- 13. The method of claim 11, wherein the biological sample comprises a culture, a fluid sample, water, a food, a beverage, or a non-biological solid.
- 14. The method of claim 11, wherein the genetic material comprises DNA or RNA.
- 15. The method of claim 11, wherein the genetic material comprises genomic DNA or mRNA.
- 16. A method of isolating and analyzing genetic material from a biological sample from a mammal, wherein the method comprises:
a. obtaining a biological sample comprising an organ or a tissue comprising cells comprising genetic material; b. dissociating the cells to produce a suspension comprising the cells; c. isolating the cells on a first solid medium; d. contacting the cells on the first solid medium with a second solid medium, wherein the second solid medium comprises a matrix having a composition sorbed thereto, wherein the composition comprises:
i. a weak base; ii. a chelating agent; and iii. an anionic surfactant or detergent; d. lysing the cells and retaining the genetic material with the second solid medium; e. analyzing the genetic material.
- 17. The method of claim 16, wherein the genetic material comprises mammalian DNA or RNA.
- 18. The method of claim 16, wherein the genetic material comprises DNA or RNA from non-mammalian cells or from viruses.
- 19. A method of isolating and analyzing genetic material from a non-solid biological sample from a mammal, wherein the method comprises:
a. obtaining a non-solid biological sample comprising a component of interest, wherein the component contains a cell, a virus, or a combination thereof and wherein the cell or the virus comprises genetic material; b. isolating the component of interest on a first solid medium; c. contacting the isolated component of interest on the first solid medium with a second solid medium, wherein the second solid medium comprises a matrix having a composition sorbed thereto, wherein the composition comprises:
i. a weak base; ii. a chelating agent; and iii. an anionic surfactant or detergent; d. releasing the genetic material from the component of interest and retaining the genetic material with the second solid medium; e. analyzing the genetic material.
- 20. The method of claim 19, wherein the genetic material comprises DNA or RNA.
- 21. A method of isolating and analyzing genetic material, wherein the method comprises:
a. obtaining a sample; b. processing the sample to produce a suspension comprising cells or virions comprising genetic material; c. isolating the cells or virions on a first solid medium; d. contacting the cells or virions on the first solid medium with a second solid medium, wherein the second solid medium comprises a matrix having a composition sorbed thereto, wherein the composition comprises:
i. a weak base; ii. a chelating agent; and iii. an anionic surfactant or detergent; e. lysing the cells or virions and retaining the genetic material with the second solid medium; and f. analyzing the genetic material.
- 22. The method of claim 21, wherein the sample comprises one of the following: an organism, an organ, a tissue, blood, plasma, serum, mucus, urine, saliva, sweat, or semen.
- 23. The method of claim 21, wherein the sample comprises a culture, a fluid sample, water, a food, a beverage, or a non-biological solid.
- 24. The method of claim 21, wherein the analysis of genetic material includes genotyping.
- 25. The method of claim 21, further comprising:
g. detecting contamination of the sample.
- 26. The method of claim 21, wherein the genetic material comprises DNA or RNA.
- 27. The method of claim 21, wherein the genetic material comprises genomic DNA or mRNA.
- 28. A method of isolating and analyzing genetic material from cells or virions, wherein the method comprises:
a. providing a first solid medium comprising cells or virions comprising genetic material; b. contacting the cells or virions on the first solid medium with a second solid medium, wherein the second solid medium comprises a matrix having a composition sorbed thereto, wherein the composition comprises:
i. a weak base; ii. a chelating agent; and iii. an anionic surfactant or detergent; c. lysing the cells or virions and retaining the genetic material with the second solid medium; and d. analyzing the genetic material.
- 29. The method of claim 28, wherein the analysis of genetic material includes genotyping.
- 30. The method of claim 28, further comprising:
e. detecting contamination of the first solid medium.
- 31. The method of claim 28, wherein the genetic material comprises DNA or RNA.
- 32. The method of claim 28, wherein the genetic material comprises genomic DNA or mRNA.
- 33. A kit for isolating genetic material, wherein the kit comprises:
a. a first solid medium capable of retaining cells or virions; b. a second solid medium, wherein the second solid medium comprises a matrix having a composition sorbed thereto, wherein the composition comprises:
i. a weak base; ii. a chelating agent; and iii. an anionic surfactant or detergent.
- 34. An apparatus for isolating genetic material, wherein the apparatus comprises:
a. a chamber for containing a fluid including a suspension of cells therein, the chamber comprising:
i. an opening therethrough; and ii. a first matrix removably disposed over the opening; b. vacuum means for drawing the fluid from the chamber and through the first matrix and depositing the cells on the matrix; c. a second matrix comprising preserving means for lysing cells and preserving genetic material sorbed to the matrix by protecting the genetic material from degradation.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application claims priority of U.S. Provisional Application Serial No. 60/248,876, filed Nov. 15, 2000.
Provisional Applications (1)
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Number |
Date |
Country |
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60248876 |
Nov 2000 |
US |