SBIR Phase I: Analysis of Combinatorial Bioproducts using Absorbance-Based, Multiplexed CE (Capillary Electrophoresis)

Information

  • NSF Award
  • 0109593
Owner
  • Award Id
    0109593
  • Award Effective Date
    7/1/2001 - 23 years ago
  • Award Expiration Date
    12/31/2001 - 23 years ago
  • Award Amount
    $ 100,000.00
  • Award Instrument
    Standard Grant

SBIR Phase I: Analysis of Combinatorial Bioproducts using Absorbance-Based, Multiplexed CE (Capillary Electrophoresis)

This Small Business Innovation Research Phase I (SBIR) project will develop high sample throughput methodologies via multiplexed capillary electrophoresis (CE) for applications in the areas of combinatorial synthesis and proteomics. CE has the potential to function as a platform for a high- throughput analysis system, but suffers from unacceptable variations in migration times and injection volumes. Furthermore, today's multiplexed CE systems use fluorescence detection only, which without labeling is ineffective in detecting ~90% of all known compounds. This project proposes the fabrication and validation of a 96-capillary array CE system that employs 1) absorbance-based detection, and 2) current measurement in each capillary. The former eliminates the need and problems with fluorescence, significantly expanding the scope of multiplexed CE. The latter uses the collected current to reduce migration time and injection volume variability to the levels required for routine analytical work. Success in Phase I will lead to a Phase II program in which the analysis system will be optimized and protocols for peptide mapping and combinatorial synthesis will be developed and standardized. <br/><br/>Potential commercial applications of this project are in markets needing high throughput screening methods. Target customers for the technology include fine chemical makers that use combinatorial techniques to develop new catalysts and other materials, pharmaceutical firms that conduct high-throughput screening in drug discovery for the development of pharmaceutical compounds, biotechnology companies that utilize peptide mapping for the rapid fingerprinting of proteins, and clinical and forensic laboratories that require high-throughput screening to analyze samples and develop evidence in criminal investigations

  • Program Officer
    Om P. Sahai
  • Min Amd Letter Date
    6/22/2001 - 23 years ago
  • Max Amd Letter Date
    6/22/2001 - 23 years ago
  • ARRA Amount

Institutions

  • Name
    CombiSep, Inc.
  • City
    Ames
  • State
    IA
  • Country
    United States
  • Address
    1915 Scholl Road
  • Postal Code
    500113020
  • Phone Number
    5112947135

Investigators

  • First Name
    Ho-ming
  • Last Name
    Pang
  • Email Address
    homing.pang@combisep.com
  • Start Date
    6/22/2001 12:00:00 AM

FOA Information

  • Name
    Industrial Technology
  • Code
    308000