SBIR Phase I: Development of a Heparinoid Affinity Matrix by Combinatorial Chemistry

Information

  • NSF Award
  • 9861229
Owner
  • Award Id
    9861229
  • Award Effective Date
    1/1/1999 - 25 years ago
  • Award Expiration Date
    6/30/1999 - 24 years ago
  • Award Amount
    $ 99,906.00
  • Award Instrument
    Standard Grant

SBIR Phase I: Development of a Heparinoid Affinity Matrix by Combinatorial Chemistry

9861229 <br/> In this SBIR Phase I application, Repligen proposes: (1) to synthesize a solid phase array of about 20,000 novel chemical structures with molecular features consistent with heparin/heparan sulfate; ( 2) to screen this biased library for those structures which show affinity for vascular endothelial growth factor (VEGF); (3) to evaluate solid phase compounds which show binding activity for VEGF in a series of secondary assays and to select those with specificity for VEGF and moderate (i.e. KD=10-7 to 10-8 M) binding affinity; and (4) to evaluate 3-5 selected solid phase compounds as potential novel affinity matrices for VEGF purification. Successful completion of the Phase I objectives of this program will produce a new tool for the purification of VEGF, a potentially important new therapeutic protein. More broadly, success in completing the Phase I objectives will establish the feasibility of this approach. <br/> General application of the technology which would be developed during Phase 2 of the proposed project can provide new affinity chromatography matrices for a wide variety of commercially important proteins. New protein purification tools such as these affinity matrices will be critical for the successful commercialization of many proteins produced through biotechnology.

  • Program Officer
    Cynthia J. Ekstein
  • Min Amd Letter Date
    10/30/1998 - 25 years ago
  • Max Amd Letter Date
    10/30/1998 - 25 years ago
  • ARRA Amount

Institutions

  • Name
    Repligen Corporation
  • City
    Needham
  • State
    MA
  • Country
    United States
  • Address
    117 Fourth Avenue
  • Postal Code
    024942725
  • Phone Number
    6173399560

Investigators

  • First Name
    Jundong
  • Last Name
    Zhang
  • Email Address
    zhang@repligen.com
  • Start Date
    10/30/1998 12:00:00 AM

FOA Information

  • Name
    Health
  • Code
    203000