Claims
- 1. A method for screening for modulators of a target protein, wherein the target protein has microtubule stimulated ATPase activity and comprises a sequence that has greater than 90% amino acid identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm, the method comprising the steps of:contacting the target protein with a candidate agent at a first concentration and determining a level of activity of the target protein; and contacting the target protein with a candidate agent at a second concentration and determining a level of activity of the target protein; wherein the activity is selected from the group consisting of binding activity or ATPase activity, and wherein a difference between the level of activity of the target protein contacted with the first concentration of the candidate agent and the level of activity of the target protein contacted with the second concentration of the candidate agent indicates that the candidate agent modulates the activity of the target protein.
- 2. The method of claim 1, wherein the screening occurs in a multi-well plate as part of a high-throughput screen.
- 3. The method of claim 1, wherein the target protein has greater than 95% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.
- 4. The method of claim 1, wherein the target protein has greater than 98% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.
- 5. The method of claim 1, wherein the target protein comprises an amino acid sequence of SEQ ID NO:2.
- 6. The method of claim 1, wherein the target protein has greater than 95% amino acid sequence identity to SEQ ID NO:4 as measured using a sequence comparison algorithm.
- 7. The method of claim 1, wherein the target protein has greater than 98% amino acid sequence identity to SEQ ID NO:4 as measured using a sequence comparison algorithm.
- 8. The method of claim 1, wherein the target protein comprises an amino acid sequence of SEQ ID NO:4.
- 9. The method of claim 1, wherein the target protein has been isolated from an endogenous source.
- 10. The method of claim 1, wherein the target protein has been produced recombinantly.
- 11. The method of claim 1, wherein said first concentration or said second concentration of the candidate agent is zero or at a level below detection.
- 12. The method of claim 1, wherein the candidate agent is an agonist.
- 13. The method of claim 1, wherein the candidate agent is an antagonist.
- 14. The method of claim 1, wherein the candidate agent binds to the target protein.
- 15. The method of claim 1, wherein the target protein is contacted with the candidate agent in vivo.
- 16. The method of claim 1, wherein the target protein is contacted with the candidate agent in vitro.
- 17. The method of claim 1, wherein determining the level of activity of the target protein comprises screening for alterations in cell cycle distribution or cell viability.
- 18. The method of claim 1, wherein determining the level of activity of the target protein comprises screening for the presence, morphology, activity, distribution, or amount of mitotic spindles.
- 19. The method of claim 1, wherein contacting the target protein with a candidate agent comprises adding the candidate agent to a mixture comprising the target protein under conditions which normally allow the production of ADP or phosphate.
- 20. The method of claim 19, wherein determining the level of activity of the target protein comprises the steps of:i) subjecting the mixture to an enzymatic reaction, wherein said enzymatic reaction uses ADP or phosphate as a substrate under conditions which normally allow the ADP or phosphate to be utilized; and ii) measuring NADH consumption as a measure of ADP production, wherein a change in measure of NADH consumption between the first and second concentrations of the candidate agent indicates that the candidate agent is a modulator of the target protein.
- 21. The method of claim 1, wherein the candidate agent is labeled.
Parent Case Info
This application is a continuation of Ser. No. 09/641,807, filed Aug. 17, 2000, the disclosure of which is incorporated by reference.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
6207403 |
Goldstein et al. |
Mar 2001 |
B1 |
Non-Patent Literature Citations (1)
Entry |
Sperry et al. Mol Biol Cell 7(2):289-305 (1996). |
Continuations (1)
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Number |
Date |
Country |
Parent |
09/641807 |
Aug 2000 |
US |
Child |
09/723096 |
|
US |