Claims
- 1. In an optical cell analysis system, a method for screening cells from a sample of cells for red blood cell abnormalities comprising the steps of:
- (a) passing the cells of said sample, one at a time in sequence, through a zone of focused illumination,
- (b) for each of said cells, developing a pulse waveform representative of predetermined light scatter from said zone;
- (c) developing respective data collections, based on given samples of cells, of pulse widths and pulse areas;
- (d) developing, for each cell, as an indicium of pulse shape, the ratio of pulse width to pulse peak, and developing a collection of pulse shape indicia;
- (e) computing respective coefficients of variation, of said pulse width, pulse area, and pulse shape indicia collections;
- (f) combining said coefficients of variation in accordance with a predetermined function, thereby producing a red cell abnormality index;
- (g) evaluating said red cell abnormality index relative to predetermined standards to identify samples having significant degrees of red cell abnormality.
- 2. A method as described in claim 1 wherein said step of combining further comprises identifying the mean of said collection of pulse shape indicia, and combining it with said coefficients of variation.
- 3. A method as described in claim 2 wherein said step of combining coefficients of variation comprises first developing an index of abnormality for each said coefficient, and then for combining those individual indices which fall outside a select range of acceptability.
Parent Case Info
This is a continuation in part of U.S. Application Ser. No. 542,221, filed Oct. 14, 1983.
US Referenced Citations (7)
Continuation in Parts (1)
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Number |
Date |
Country |
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542221 |
Oct 1983 |
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