Patent Application
20240342678
The invention relates to assays for microwell analysis. More particularly, the invention relates to providing sealed microwell assays for the analysis of biological samples in small volumes.
Microwell arrays for biological assays have been in use for some time. One of the applications for microwell arrays is the determination of the expression profiles for single cells. For example, U.S. Patent Application Publication No. 2016/0289669 by Fan et al. discloses microwell-based devices and systems for single cell expression profile analysis wherein single cells and single ligand-coated beads are loaded into a microwell and permitted sediment to the bottom of the microwell prior to conducting an analysis on the biological activity of the cells. However, the microwells remain in an unsealed state while the analysis is performed.
The device and systems of Fan et al. produce a number of deficiencies. First, the unsealed state of the microwells permits the uncontrolled exchange of targeted biomolecules between microwells resulting in microwell cross-contamination and compromised accuracy of the single cell analysis. As shown by Yuan and Sims (Scientific Reports 6, Article number: 33883 (2016)), this problem can be mitigated by covering microwells with a layer of oil which is immiscible in water, but the assay becomes more complicated as a result. A microwell-based assay described in a recent publication (Gierahn et al., Nat Methods. 2017 April; 14 (4): 395-398) attempts to provide a sealed microwell configuration through the use of a semipermeable membrane. However, this approach also complicates microwell analysis by requiring additional steps in the application of its microwell technology. Second, in a microwell configuration where a bead and a cell are placed next to each other, the volume of the medium into which the content of the cell is released is relatively large compared to the volume of the cell, resulting in substantial dilution of the released biomolecules and, hence, reduced binding efficiency to the ligands on the bead. This limitation is also inherent to the semipermeable membrane-based approach to the sealing of microwells. Third, it is difficult to pair exactly one bead to one cell in a microwell configuration when the bead and the cell are placed next to each other on the bottom of a microwell. Fourth, the distance between the bead and cell usually varies among microwells in the array, resulting in additional microwell-to-microwell variability in the binding efficiency between targeted biomolecules and the beads. Thus, there exists a need for improving the accuracy, ease of use, and efficiency of contemporary microwell-based assays, including microwell-based assays for single cell analysis.
The invention provides systems, methods, devices and kits for enhancing the accuracy and efficiency of microwell-based assays. The invention enhances the accuracy and efficiency of microwell analysis by enclosing samples within small volumes in microwells through a novel geometric pairing of microwells and beads. This geometric pairing provides a number of advantages. First, enclosing the sample prevents the cross-contamination of materials between neighboring microwells. Second, enclosing the sample through the geometric pairing of microwells and beads enables a reduction of the effective volume of medium carrying the sample, thus increasing the concentration of the sample and hence, the ligand binding efficiency. Third, the geometric pairing of microwells and beads only allows one bead to enter a microwell, thus preventing a situation when a cell in a microwell is paired with more than one bead. Finally, the position of the beads with respect to cells in the microwells remains highly consistent.
The invention relates to improving the efficiency and accuracy of microwell analysis. More particularly, the invention relates to systems, methods, devices and kits for improving the efficiency and accuracy of microwell analysis.
Existing microwell systems suffer serious limitations in terms of their ability to efficiently and accurately analyze samples in individual microwells within an array. These limitations result, in part, from the lack of a means for enclosing samples within individual microwells within an array. The lack of a sealing functionality permits targeted analytes to diffuse or flow from one well to another, leading to cross-contamination which compromises the analysis of samples. This shortcoming can be particularly problematic in the context of performing single cell expression profile analysis, such as in the systems and methods disclosed in U.S. Patent Application Publication No. 2016/0289669 to Fan et al. Fortunately, the inventor recognized that these limitations in microwell analysis can be overcome through a novel combination of microwell and bead geometry that permits samples to be effectively sealed within a small analytical space.
The inventor surprisingly discovered that a sealed analytical space within a microwell can be formed by pairing the geometry of the microwell with the geometry of a bead such that the surface of the bead contacts the walls of the microwell thereby forming a seal at the microwell-bead interface. The sealed analytical space provides a number of advantages over contemporary microwell analysis. Sealing of the analytical space prevents, or at least inhibits, the egress and ingress of targeted analytes from and to individual microwells, thereby improving assay accuracy by preventing cross-contamination of neighboring microwells within an array. In some embodiments, the geometric pairing of microwells and beads can allow only one bead to enter a microwell, thus preventing a situation when a cell in a microwell is paired with more than one bead.
In at least some embodiments of the invention, a sealed analytical space is achieved by pairing the geometry of the microwell and the geometry of the bead such that the width of the bead is greater than the width of the bottom of the microwell. Accordingly, microwells for use with the invention can assume any geometric shape wherein the top (i.e. opening) of the microwell has a width that is greater than the width of the bottom of the microwell. Suitable geometric shapes for the microwells, include, but are not limited to, cylinders, polyhedrons, inverted cones, inverted pyramids, hemispheres, or combinations thereof. Suitable shapes for the microwells include, but are not limited to, those depicted in
At least one aspect of the invention concerns the dimensions of the microwells. A non-limiting description of the relative dimensions of the microwells is depicted in
In some preferred embodiments, the width of the top (i.e. opening) of the microwells is proportionately greater than the width of the bottom of the microwells. Referring to
While a conical frustum is depicted in
In some embodiments, the walls of the microwells may be slanted wherein a positive draft angle gives rise to a larger opening at the top of the well. In some embodiments, the walls may be slanted positively or negatively by at least 1 degree, at least 2 degrees, at least 3 degrees, at least 4 degrees, at least 5 degrees, at least 6 degrees, at least 7 degrees, at least 8 degrees, at least 9 degrees, at least 10 degrees, at least 11 degrees, at least 12 degrees, at least 13 degrees, at least 14 degrees, or at least 15 or more degrees. In some embodiments, the walls may be slanted positively or negatively by at most 1 degree, at most 2 degrees, at most 3 degrees, at most 4 degrees, at most 5 degrees, at most 6 degrees, at most 7 degrees, at most 8 degrees, at most 9 degrees, at most 10 degrees, at most 11 degrees, at most 12 degrees, at most 13 degrees, at most 15 degrees, or at most 15 or more degrees. In these embodiments, therefore, the top of the microwell can have a different diameter (or average diameter) than the bottom of the microwells. In some preferred embodiments, the walls are slanted by a positive draft angle in the range of about 10 to about 30 degrees. In some embodiments, the opening angle of the microwells is two times greater than the average draft angle. The opening angle of the microwells can be between about 0° and about 60°. The opening angle can be up to 60°, up to 55°, up to 50°, up to 45°, up to 40°, up to 35°, up to 30°, up to 25°, up to 20°, up to 15°, up to 10°, up to 5°, up to 1°, or up to any opening angle that intervenes these specifically listed angles. The opening angle can be up to about 10°, about 11°, about 12°, about 13º, about 14°, about 15°, about 16°, about 17°, about 18°, about 19º, about 20°, about 21°, about 22°, about 23°, about 24°, about 25°, about 26°, about 27°, about 28°, about 29º, about 30°, about 31°, about 32°, about 33°, about 34º, about 35°, about 36°, about 37°, about 38°, about 39°, about 40°, about 41º, about 42°, about 43°, about 44°, about 45°, about 46°, about 47°, about 48°, about 49°, about 50°, about 51º, about 52°, about 53º, about 54°, about 55°, about 56°, about 57°, about 58°, about 59°, or about 60°. In some preferred embodiments, the opening angle is 34° or 35°.
At least one aspect of the invention concerns the geometry of the beads that are paired with the microwells. The beads can be spherical or substantially spherical. The beads can be microspheres. The beads can have a width (i.e. diameter) that is between about 10 μm and about 100 μm. The beads width can be at least 10 μm, at least 15 μm, at least 20 μm, at least 25 μm, at least 30 μm, at least 35 μm, at least 40 μm, at least 45 μm, at least 50 μm, at least 55 μm, at least 60 μm, at least 65 μm, at least 70 μm, at least 75 μm, at least 80 μm, at least 85 μm, at least 90 μm, at least 95 μm, at least 100 μm, or at least a width that intervenes these specific widths. The beads can have a width up to 10 μm, up to 15 μm, up to 20 μm, up to 25 μm, up to 30 μm, up to 35 μm, up to 40 μm, up to 45 μm, up to 50 μm, up to 55 μm, up to 60 μm, up to 65 μm, up to 70 μm, up to 75 μm, up to 80 μm, up to 85 μm, up to 90 μm, up to 95 μm, up to 100 μm, or up to a width that intervenes these specific widths. For the geometric pairing of the microwell with the bead, the ratio of the width of the bottom of the microwell to the width of the bead can be any ratio wherein the width of the bead is proportionately greater than the width of the bottom of the microwell. The ratio of the width of the bottom of the microwell to the width of the bead can range from about 0.05 to about 0.99. In some embodiments, the ratio of the width of the bottom of the microwell to the width of the bead is at most 0.99, at most 0.95, at most 0.9, at most 0.8, at most 0.7, at most 0.6, at most 0.5, at most 0.4, at most 0.3, at most 0.2, at most 0.1, or at most 0.05. One skilled in the art will appreciate that the ratio can be any ratio that intervenes those listed here, such as 0.37. One skilled in the art will further appreciate that the bead widths referenced herein can refer to a mean bead diameter in instances where a plurality of beads are contemplated.
Beads for use with the invention can be fabricated from a material selected from the group consisting of polymethylmethacrylate, epoxy, silicon, fused-silica, glass, a polymer, a metal, alumina, an elastomer, polydimethylsiloxane, agarose, and a hydrogel, or any combination thereof. Examples of beads suitable for use with the invention include, but are not limited to, streptavidin conjugated beads, agarose beads, magnetic beads, glass beads, Dynabeads®, MACS® microbeads, antibody conjugated beads (e.g., anti-immunoglobulin microbead), protein A conjugated beads, protein G conjugated beads, protein A/G conjugated beads, protein L conjugated beads, oligo-dT conjugated beads, silica beads, silica-like beads, anti-biotin microbeads, and BcMag™ Carboxy-Terminated Magnetic Beads. The beads can be polymethylmethacrylate microspheres. The beads can have a density of between about 1 g/cm3 and about 4 g/cm3. The beads can have a density of up to 2 g/cm3, up to 3 g/cm3, up to 4 g/cm3, or up to any density that intervenes the specifically listed densities. The beads can have a density of at least 1 g/cm3, at least 2 g/cm3, at least 3 g/cm3, at least 4 g/cm3, or at least any density that intervenes these specifically listed densities. The beads can be associated with (e.g. impregnated with) quantum dots or fluorescent dyes to make them fluorescent in one fluorescence optical channel or multiple optical channels. The beads can be magnetic. The beads can be associated with iron oxide or chromium oxide to make them paramagnetic or ferromagnetic. The beads can be functionalized. When used in reference to a bead, the terms “functionalized”, “functional” and the like, refer to a bead having at least one ligand on its surface for capturing a targeted analyte. The ligand can be an antibody, peptide, protein, an oligonucleotide, or combinations thereof. The beads can be non-spherical in shape, such as an elliptical shape having an elliptical longitudinal cross-section and a circular transverse cross-section.
While a particular pairing of a bead and microwell geometry is shown in
As shown in
It will be appreciated by one skilled in the art that the geometric pairing of the microwell and the bead can be adjusted to achieve a desired distance between the bottom of the microwell and the bottom of a bead that is nested within the microwell. For example, the skilled artisan may desire to adjust the distance between the bottom of the microwell and the bottom of the nested bead based on the average diameter of a cell to be analyzed so as to bring ligands on the surface of a functionalized bead into a close proximity to the cell. Similarly, the skilled artisan may desire to decrease the volume of the analytical space to increase the concentration of an analyte within the reaction mixture. Referring to
Referring to
The geometric pairing of the microwell and bead can be designed to provide contact between the bead and microwell at a desired periphery of the bead surface. The periphery can be the periphery (i.e. outer surface) of a spherical bead. The geometric pairing can result in contact between the microwell walls and the periphery of the bead, wherein the width (e.g. diameter) of the bead periphery in contact with the walls of the microwell is equal to, or less than, the width (e.g. diameter) of the bead. It will be understood that the periphery of the bead in contact with the walls of the microwell equates to the circumference of the bead in the horizontal plane where the bead contacts the walls of the microwell. The ratio between the width of the bead and the width of the bead periphery in contact with the walls of the microwell can be up to 1.0, up to 0.9, up to 0.8, up to 0.7, up to 0.6, up to 0.5, up to 0.4, up to 0.3, up to 0.2, up to 0.1, or up to any ratio that intervenes these specifically listed ratios. The geometric pairing of the microwell and the bead can result in contact between the periphery of the bead and the walls of the microwell at the top (i.e. opening) of the microwell. The geometric pairing of the microwell and bead can result in contact between the periphery of the bead and the walls of the microwell below the top of the microwell. The geometric pairing can result in contact between the periphery of the bead and the walls of the microwell, wherein at least a portion of the bead protrudes above the top of the microwell. The height of the portion of the bead that protrudes above the top of the microwell can be greater than, equal to, or less than, the radius of the bead. The ratio between the height of the portion of the bead that protrudes above the top of the microwell and the radius of the bead can be up to 2.0, up to 1.9, up to 1.8, up to 1.7, up to 1.6, up to 1.5, up to 1.4, up to 1.3, up to 1.2, up to 1.1, up to 1.0, up to 0.9, up to 0.8, up to 0.7, up to 0.6, up to 0.5, up to 0.4, up to 0.3, up to 0.2, up to 0.1, or up to any ratio that intervenes these specifically listed ratios. The portion of the bead that protrudes above the top of the microwell can be up to 100% of the volume of the bead, up to 90% of the volume of the bead, up to 80% of the volume of the bead, up to 70% of the volume of the bead, up to 60% of the volume of the bead, up to 50% of the volume of the bead, up to 40% of the volume of the bead, up to 30% of the volume of the bead, up to 20% of the volume of the bead, up to 10% of the volume of the bead, up to 5% of the volume of the bead, up to 1% of the volume of the bead, or any percentage volume that intervenes these specifically listed percentages. The geometric pairing of the bead and microwell can result in contact between the periphery of the bead and the walls of the microwell, wherein the top of the bead lies below the plane that defines the top of the microwell.
Some non-limiting embodiments for the geometric pairing of the microwell and bead are shown in
In some embodiments, the geometrical pairing between the bead and the microwell is such that a microwell can only accommodate a single bead. This single bead to microwell functionality can be achieved in embodiments wherein the top of an anchored bead protrudes above the top (i.e. opening) of the microwell such as depicted in
In at least some embodiments of the invention, a plurality of microwells as disclosed herein are formed within a substrate to provide a microwell array. In some embodiments, the substrate comprises from 100 to 100,000,000 microwells. In some embodiments, the substrate comprises from 100 to 50,000,000 microwells. In some embodiments, the substrate comprises up to 100,000,000 microwells. In some embodiments, the substrate comprises up to 50,000,000 microwells. In some embodiments, the substrate comprises from 10,000 to 200,000 microwells. The substrate can be fabricated from a material selected from the group consisting of silicon, fused-silica, glass, a polymer, a metal, an elastomer, polydimethylsiloxane, agarose, and a hydrogel, or any combination thereof. One skilled in the art will appreciate that when a plurality of the microwells disclosed herein are incorporated into a substrate to form a microwell array, the dimensions of the microwells described herein can refer to the average or mean dimensions of such plurality of microwells.
The present invention provides methods for analyzing the bioactivity in a sample, and methods for identifying the presence of an analyte in a sample. The methods can be practiced by providing a substrate comprising at least one microwell, loading a sample into the microwell, and loading at least one bead onto the substrate. The geometry of the microwell and bead can be paired such that loading the bead onto the substrate results in the bead nesting within the microwell and contacting the walls of the microwell to form an interface that encloses the sample within a space between the bead and the bottom of the microwell. The substrate can be an array of microwells as disclosed herein. The microwells can be one or more of the microwells disclosed herein. The beads can be the beads disclosed herein.
In at least one embodiment, the invention provides a method for analyzing the bioactivity of a sample. Analyzing the bioactivity of a sample can comprise detecting the presence or absence of one or more analytes in the sample. Analyzing the bioactivity of the sample can comprise determining the amount of one or more analytes in the sample. The analyte can be a biomolecule, wherein analyzing the activity of the sample comprises identifying the presence or absence of one or more biomolecules in the sample. The analyte can be a biomolecule, wherein analyzing the activity of the sample comprises determining the amount of one or more biomolecules in the sample. The terms “product” and “analyte” are used interchangeably herein to refer to a substance that is of interest (i.e. targeted) for analysis. The analyte can be a biomolecule. As used herein, the term “biomolecule” includes, but is not limited to, proteins, polypeptides, oligopeptides, peptides, polynucleotides, oligonucleotides, nucleotides, nucleic acids (e.g., including DNA (such as cDNA and genomic DNA) and RNA (such as mRNA and tRNA)), polysaccharides, oligosaccharides, lipids, antibody (e.g. heavy and light polypeptide chain antibody), cell receptors, low weight molecules (e.g., hormones, ligands, signal transduction substances, low-weight organic molecules, etc.), and complex molecules thereof, and the like. In some embodiments, the biomolecule is mRNA. In some embodiments, determining the bioactivity of a sample comprises determining the number of copies of at least one oligonucleotide in the sample. In some embodiments, determining the bioactivity of a sample comprises determining the number of copies of at least one oligonucleotide in the sample, wherein the sample is a cell. In some embodiments, the analyte can be a cell, virus, organelle, or part of a tissue. In some embodiments, the analyte can be a plurality of cells, a plurality of viruses, a plurality of organelles, or plurality of tissue specimens.
The beads can be loaded onto the substrate (i.e. microwell array) as a suspension with the beads suspended in a liquid carrier. The beads can have a greater density than the liquid carrier so that individual beads settle into individual microwells when the suspension is loaded onto the microwell array. The number of beads loaded onto the substrate can be proportionately greater than, equal to, or proportionately less than, the number of microwells in the array. The number of beads loaded onto the microwell array can be smaller than the number of microwells to limit the number of beads that are not anchored inside microwells. The number of beads loaded onto the substrate can be greater than the number of microwells in the substrate to minimize the number of microwells having no beads anchored within them. The beads can be loaded onto the substrate in a bead-to-microwell ratio of 1:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1 or 1.5:1. As noted herein, the geometric pairing of the bead and microwell can facilitate the loading of single beads within individual microwells in an array. In embodiments wherein at least a portion of an anchored bead protrudes above the top of the microwell, the portion of the bead protruding above the microwell can prevent other beads from entering the microwell. In embodiments wherein the upper surface of the bead lies below the plane of the top of the microwell, a second bead can rest on top of the bead that is already anchored in the microwell. Nevertheless, the forces holding this second bead can be sufficiently weak so that only minimal agitation, such as originating from convective flow, is required to remove the second bead from its position on top of the anchored bead without dislodging the anchored bead.
The sample can be a cell or a plurality of cells. The sample can be a plurality of clonal cells, or a plurality of mixed cells. Suitable samples for analysis by the disclosed methods, systems and kits of the invention include any sample comprising a plurality of cells, such as, for example, cell cultures, blood samples, tissue samples in which the extracellular matrix has been digested or dissolved to release individual cells into suspension, and the like. The plurality of cells may be derived from a single sample, or from two or more samples that have been combined, and may comprise a plurality of cells of the same type, or a plurality of cells of mixed type. The cells can be mammalian cells. The cells can be human, mouse, rat, rabbit, goat, pig, or guinea pig cells. In some embodiments, the cells are human cells. In some embodiments, the cells are other types of animal, plant, fungal, bacterial, or protist cells. The cells can be any prokaryotic or eukaryotic cells.
In some embodiments, the cells are loaded onto the substrate at a concentration that reduces the probability of more than one cell being loaded into an individual microwell within the substrate. Loading of no more than one cell into individual microwells can be achieved by loading onto the substrate a cell suspension that contains fewer cells than the number of microwells in the substrate. In some preferred embodiments, the concentration of the cell suspension is adjusted so that the volume of the cell suspension used to load the substrate contains approximately one-tenth the number of cells as the number of microwells within the substrate. The cell suspension loaded onto the substrate can contain approximately one fourth, one fifth, one sixth, one seventh, one eighth, one ninth, or one tenth the number of microwells that are contained within the substrate. In some embodiments, loading comprises loading the cells such that no more than 20% of the cells are in microwells with other cells. In some embodiments, loading comprises loading the cells such that no more than 15% of the cells are in microwells with other cells. In some embodiments, loading comprises loading the cells such that no more than 10% of the cells are in microwells with other cells. In some embodiments, the loading comprises loading the cells such that no more than 5% of the cells are in microwells with other cells. In some embodiments, the loading comprises loading the cells such that no more than 1% of the cells are in microwells with other cells. The probability to find a given number of cells in a single microwell is typically governed by Poisson statistics.
In some embodiments, the invention provides methods of identifying the presence of an analyte. The analyte can be one or more biomolecules. The biomolecules can be oligonucleotide molecules, DNA molecules, RNA molecules, mRNA molecules, micro RNA molecules, tRNA molecules, and the like. In some embodiments, the biomolecules can be peptides or proteins. The biomolecules can be antibody heavy and light polypeptide chains, and/or receptor polypeptide chains (e.g., the alpha and beta chains of the T cell receptor). The analyte can be selected from the group consisting of antibodies, proteins, peptides, lipids, lipoproteins, amino acids, carbohydrates, oligonucleotides, viruses, exosomes, or a combination thereof.
In at least some embodiments, the beads have one or more ligands for capturing (i.e. binding to) one or more analytes. The beads can have one or more copies of different ligands for capturing different target analytes. The ligands can be attached to the beads by covalent or non-covalent means. The ligands can be antibodies, proteins, peptides, oligonucleotides, or a combination thereof. The beads can comprise one or more nucleotides for conducting single cell expression analysis. Beads for single cell expression analysis can comprise (i) a cell identifying marker that is unique to each bead within a plurality of beads, and (ii) at least one ligand for binding a biomolecule of interest (i.e. analyte). Beads for single cell expression analysis can comprise a moiety wherein each moiety comprises (i) a cell identifying marker that is unique to each bead within a plurality of beads, (ii) a ligand for binding a biomolecule of interest (i.e. analyte), and (iii) a ligand index marker that is unique for each moiety. Beads for single cell expression analysis can comprise a plurality of capture ligands capable of hybridizing to a target nucleic acid, wherein each of the capture oligonucleotides comprises (i) an oligonucleotide cell marker that is unique to each bead, and (ii) an oligonucleotide label portion that is unique to each capture oligonucleotide. Suitable bead moieties for conducting single cell expression analysis according to the methods, system and kits of the invention include, but are not limited to, those disclosed in U.S. Patent Application Publication No. 2016/0289669, the entire contents of which are incorporated herein by reference for all purposes. One non-limiting embodiment of a bead for conducting single cell expression analysis is shown in
In some embodiments, the analyte is secreted from a cell after the cell is enclosed in the analytical space between the bead and the bottom of the microwell. The cell can be incubated under conditions sufficient to permit the cell to secrete the analyte into the analytical space. The secreted analyte can be a biomolecule. The secreted analyte can be, for example, a cytokine, chemokine, mRNA, exosome, peptide, protein, glycoprotein, antibody, or amino acid. In some embodiments, the analyte can be released from the cells by cleavage (e.g. enzymatic cleavage). In some embodiments, cells within the sample are lysed after being enclosed between the beads and the bottom of the microwells and prior to conducting the analysis. Cell lysis may be implemented in assays wherein the analyte is an intracellular biomolecule. Cell lysis can be accomplished by chemical or biochemical means, by osmotic shock, or by means of thermal lysis, mechanical lysis, or optical lysis. In some embodiments, for example, cells may be lysed by addition of a cell lysis buffer comprising a detergent (e.g. SDS, Li dodecyl sulfate, Triton X-100, Tween-20, or NP-40), an organic solvent (e.g. methanol or acetone), or combinations thereof.
In some embodiments, the invention provides a method for determining the number of occurrences of a target nucleic acid in individual cells in a sample of cells. The method can be practiced by loading a sample of cells onto an array comprising a plurality of microwells, and loading a bead library onto the array. The geometry of the microwells and the beads in the library is paired such that the width of the beads is greater than the width of the bottom of the microwells. The geometry of the beads and microwells can be paired as specifically disclosed herein. Due to the geometric pairing of the beads and microwells, loading of the beads into the microwells results in the enclosure of individual cells within the microwells in small volumes in the space between the bottoms of individual beads and the bottoms of the microwells. The beads in the bead library can comprise a plurality of oligonucleotide ligands for capturing at least one target oligonucleotide. The oligonucleotide ligands can be conjugated to (i) an oligonucleotide cell marker that is unique to each bead, and (ii) an oligonucleotide label that is unique to each occurrence of the oligonucleotide ligand. One non-limiting embodiment for a bead comprising the oligonucleotide ligand is depicted in
Once loaded into the microwell and enclosed in the space between the bottom of the bead and the bottom of the microwell, the individual cells can be incubated under conditions sufficient to permit the cells to secrete one or more target oligonucleotides. In some embodiments, the cells are lysed so as to release the target oligonucleotides into the small volume in the space between the bottom of the bead and the bottom of the microwell. With the target oligonucleotide in the space between the bottom of the bead and the bottom of the microwell, the target oligonucleotide is permitted to hybridize to the oligonucleotide ligand. The beads, with the target oligonucleotide hybridized to the oligonucleotide ligand, are collected. In some embodiments, the beads are magnetic and are collected using a magnet. In some embodiments, the beads are collected by dislodging the beads from the microwells through the use of a buffer solution that has higher density than the beads. Once the beads are collected, the hybridized target oligonucleotides are subjected to amplification to create a plurality of oligonucleotide conjugates that comprise (i) the hybridized target nucleic acid, (ii) an oligonucleotide that is complimentary to the oligonucleotide cell marker, and the (iii) the oligonucleotide label. The amplified oligonucleotide conjugates are then used to determine the number of occurrences of the target oligonucleotides that are expressed by individual cells within the cell sample. Methods for amplifying the oligonucleotide conjugates and correlating them with the number of occurrences of the target oligonucleotides expressed by individual cells are disclosed in the following references, the entire contents of which are incorporated herein by reference in their entirety for all purposes: U.S. Patent Application Publication No. 2016/0289669; Bose et al. Genome Biology (2015) 16:120; and Fan et al. Science (2015) Vol 347, Issue 6222, P. 1258367-1.
In at least one embodiment, the invention provides a method for loading a sample into a microwell. The method can be practiced by geometrically pairing a bead and microwell as disclosed herein, loading a sample into the microwell, and loading the bead into the microwell, wherein loading the bead into the microwell contacts the periphery of the bead with the walls of the microwell thereby anchoring the bead within the microwell and enclosing the sample within a space between bottom of the anchored bead and the bottom of the microwell. The sample can then be analyzed for the presence of one or more analytes, including determining the number of occurrences and/or concentration of the analytes. The sample can be assayed for its bioactivity. The bead can be contained within a plurality of beads that are loaded onto a substrate comprising a plurality of the microwells.
In at least one embodiment, the invention provides a kit for analyzing the bioactivity of a sample. The kit can be used for determining the presence and/or amount of one or more analytes in a sample. The kit can comprise a microwell and bead that are geometrically paired as disclosed herein such that loading the bead into the microwell contacts the periphery of the bead with the walls of the microwell thereby creating an enclosed space between the bottom of the microwell and the surface of the bottom part of the bead down from the interface formed between the periphery of the bead and the walls of the microwell. The kit can comprise a plurality of beads and a plurality of microwells within a substrate. The plurality of beads can be suspended within a liquid carrier. The beads can be conjugated to a ligand for binding an analyte (e.g. biomolecule) of interest as disclosed herein. The kit can comprise a plurality of beads and a separate liquid carrier for creating a suspension that comprises the plurality of beads. The beads can contain one or more ligands for capturing a targeted analyte as disclosed herein. The kit can contain one or more ligands and reagents for attaching the ligands to the beads. The kit and its individual components can be placed within a container, wrap (e.g. shrink wrap), bag or other suitable packaging, and can include instructions for analyzing the bioactivity of a sample, including detecting the presence and/or amount of one or more analytes contained within the sample.
An array of microwells with shapes of polygonal or conical frusta, with diameters of 10-50 μm at the bottom, 50-130 μm at the top, and 30-150 μm height is used. The microwells are filled with a medium and a suspension of cells is loaded onto the array. Cells are typically loaded in a ratio of 1:10 to the microwells, while an effort is made to distribute cells as evenly as possible over the area of the array. Cells are allowed sufficient time to sediment onto bottoms of the microwells. After that, a suspension of beads with a near-uniform diameter in a 10-100 μm range is loaded onto the microwell array, typically at a bead to microwell ratio of 1.1:1 to 1.5:1. Surfaces of the beads are functionalized, typically with oligonucleotides or antibodies, to bind molecules released by cells. Sufficient time is allowed for beads to settle into the microwells, and an effort is made to spread beads as uniformly as possible over the array, making sure that there is a bead in every microwell. The next step is different for different types of assays. If the goal is to capture cellular content, cells in the microwells are lysed and sufficient time is allowed for molecules released from cells to bind to beads. If the goal is to capture molecules secreted by live cells, sufficient incubation time is allowed to capture these molecules on the beads, with optional stimulation of cells during the incubation. Finally, the analysis of molecules captured on the beads is performed either inside or outside the microwell array.
The practicality of sealing the conical microwells with beads was tested in an experiment with an array of microwells with shapes of conical frusta with 140 μm diameters at the bottom, 200 μm depths, and 34° opening angles such as depicted in
To obtain a more quantitative estimate of the quality of sealing of the microwells by the beads, diffusion of fluorescein from under beads in microwells was numerically simulated in Comsol. In the case of a solid 225 μm diameter bead with a 0.05 μm gap between the bead and the conical sidewalls of a microwell with the shape and dimensions as in the experiment, the simulation predicted the concentration of fluorescein under the bead to decay to 50% after ˜6 min (thick curve,
Configuring microwells in a conical shape can ensure that only one bead enters each microwell. In tests of conically shaped microwell arrays that involved >10,000 of monodisperse beads, it was never observed that more than one bead entered a microwell.
Additionally, when in a conical microwell, a bead rests on the conical sidewalls and is positioned at a well-defined, small distance above the microwell bottom. When the parameters of the microwells and diameter of the bead are properly chosen, the volume of medium between the bottom of the bead and the cell (or cells) on the microwell bottom can be reduced to a minimum, while avoiding the unwanted physical contact between the bead and the cell (cells). This feature makes microwells of the invention advantageous for assays involving hybridization of molecules released by cells or from lysed cells with molecules carried by beads. Because the hybridization occurs in a minimal volume with a proportionally high concentration of molecules released by cells (or from cells), an efficient and fast binding of target molecules to the beads can be achieved.
As a demonstration of this principle, microwells were made with 15 μm diameter at the bottom. ˜60 μm depth, and 34° opening angles, and monodisperse 30 μm diameter beads were loaded onto the microwell array. The volume of medium between the bead and microwell bottom was <5 pL, while the bottom of the bead was >10 μm above the bottom of the microwell, providing substantial clearance between the bead and a cell at the microwell bottom. As a comparison, in the microwell arrays used for single-cell RNA-sequencing which are covered by a semipermeable membrane as taught by Gierahn et al. (Nat Methods. 2017 April; 14 (4): 395-398), the volume of medium in the microwells (where hybridization is designed to occur) was ˜50 pL which is 10-times greater and is expected to result in 10-fold lower concentration of molecules released by cells (or from cells) and a substantially slower and less efficient hybridization. Moreover, in droplet-based assays, a cell and a bead are typically confined in a droplet of medium with a volume of 1-2 nL, making the concentration of molecules released from the cell at least two orders of magnitudes lower than in the implementation of the microwell configuration of the invention.
All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference in its entirety. In the event of a conflict between a term herein and a term in an incorporated reference, the term herein controls.
This application is a continuation of application Ser. No. 15/927,057, filed Mar. 20, 2018, which claims priority to provisional application No. 62/474,283, filed Mar. 21, 2017. The entire contents of these applications are incorporated herein by reference for all purposes.
| Number | Date | Country | |
|---|---|---|---|
| 62474283 | Mar 2017 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15927057 | Mar 2018 | US |
| Child | 18620775 | US |
