Claims
- 1. A plasmid comprising a DNA sequence encoding the kil gene as set forth in FIG. 2 directly and operably linked to the Ex promoter derived from Bacillus sp. No. 170.
- 2. The plasmid according to claim 1, wherein said Ex promoter has a nucleotide sequence as shown in FIG. 3.
- 3. A process for producing a heterologous protein, which comprises:
- (a) transforming a microorganism with a plasmid wherein said plasmid comprises (i) the kil gene of FIG. 2 directly and operatively linked to the Ex promoter derived from Bacillus sp. No. 170, (ii) a genetic marker, and (iii) a DNA fragment encoding a heterologous protein;
- (b) culturing said microorganism under suitable conditions for the production and secretion of the heterologous protein to the culture medium; and
- (c) isolating the heterologous protein from the culture medium.
- 4. The process according to claim 3, wherein the heterologous protein is xylanase.
- 5. The process according to claim 3, wherein the heterologous protein is cellulase.
- 6. The process according to claim 3, wherein the transformed microorganism is Escherichia coli HB101 (p7AX2).
- 7. The process according to claim 3, wherein the transformed microorganism is Escherichia coli HB101 (p7NK1).
- 8. The process according to claim 3, wherein the transformed microorganism is Escherichia coli HB101 (p7FK1).
- 9. The process according to claim 3, wherein said plasmid containing the DNA fragment coding for the heterologous protein is a plasmid selected from the group consisting of p7AX2, p7FK1 and p7NK1.
- 10. Escherichia coli HB101 (pEAP7.DELTA.P).
- 11. Escherichia coli HB101 (p7AX2).
- 12. Escherichia coli HB101 (p7NK1).
- 13. Escherichia coli HB101 (p7FK1).
Priority Claims (1)
Number |
Date |
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60-168288 |
Jul 1985 |
JPX |
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CROSS REFERENCE TO RELATED APPLICATION
This application is a continuation of application Ser. No. 07/557,983, filed Jul. 26, 1990, now abandoned, which, in turn, is a continuation of application Ser. No. 06/891,242, filed Jul. 29, 1986, now abandoned.
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4624922 |
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4948735 |
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Non-Patent Literature Citations (6)
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Continuations (2)
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Parent |
557983 |
Jul 1990 |
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Parent |
891242 |
Jul 1986 |
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