Claims
- 1. A method for selective inactivation of an Aspergillus protease in a mixture of enzymes containing at least one a desirable non-Aspergillus enzyme and an acid labile protease, wherein the non-Aspergillus enzymes are recombinantly produced in an Aspergillus host that expresses said acid-labile protease, said method comprising the step of holding the mixture at a temperature of from 2.degree. C. to 75.degree. C. at a pH below 4.5 for at least 20 seconds.
- 2. A method according to claim 1, wherein said at least one desirable enzyme is selected from the group consisting of lipases, amylases, cellulases, oxidoreductases, xylanases, isomerases, proteases and peptidases.
- 3. A method according to claim 1, wherein the mixture of enzymes is contained in a culture broth.
- 4. A method according to claim 3, wherein the mixture of enzymes is contained in a clarified and concentrated culture broth.
- 5. A method according to claim 1, wherein said mixture of enzymes is adjusted to a pH below 4.0.
- 6. A method according to claim 1, wherein said temperature is in the range of from 10-70.degree. C.
- 7. A method according to claim 1, wherein, after said inactivation, the level of the Aspergillus protease is less than 15% of its initial level.
- 8. A method according to claim 1, wherein, after said inactivation, the level of the desirable enzyme is more than 50% of its initial level.
- 9. A method according to claim 1, wherein, after said inactivation, the level of the Aspergillus protease is less than 15% of its initial level and the level of the desirable enzyme is more than 50% of its initial level.
- 10. A method according to claim 1, wherein two or more enzymes are desirable.
- 11. A method according to claim 1, further comprising, prior to said holding step, adding to said mixture at least one stabilizer of said desirable enzyme.
- 12. A method according to claim 1, further comprising, prior to said holding step, adding to said mixture at least one destabilizer of said Aspergillus protease.
- 13. A method according to claim 1, further comprising, prior to said holding step, adding to said mixture at least one stabilizer of said at least one desirable enzyme and at least one destabilizer of said Aspergillus protease.
Priority Claims (1)
Number |
Date |
Country |
Kind |
1392/95 |
Dec 1995 |
DKX |
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CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of PCT/DK96/00489 filed Nov. 25, 1996 and claims priority under 35 U.S.C. 119 of Danish application 1392/95 filed Dec. 7, 1995, the contents of which are fully incorporated herein by reference.
US Referenced Citations (13)
Foreign Referenced Citations (4)
Number |
Date |
Country |
0 021 179 |
Jan 1981 |
EPX |
1281232 |
May 1962 |
FRX |
9013638 |
Nov 1990 |
WOX |
9720921 |
Jun 1997 |
WOX |
Continuations (1)
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Number |
Date |
Country |
Parent |
PCTDK9600489 |
Nov 1996 |
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