Claims
- 1. A HCV self-replicating polynucleotide comprising:
(a) a 5′-NTR consisting of ACCAGC (SEQ ID NO. 8); (b) a HCV polyprotein region coding for a HCV polyprotein; and (c) a 3′-NTR region.
- 2. The HCV polynucleotide according to claim 1, wherein said polyprotein comprises one or more amino acid substitution selected from the group consisting of: R(1135)K, S(1148)G, S(1560)G, K(1691)R, L(1701)F, I(1984)V, T(1993)A, G(2042)C, G(2042)R, S(2404)P, L(2155)P, P(2166)L and M(2992)T.
- 3. The HCV polynucleotide encoding a polyprotein comprising one or more of the amino acid substitutions as defined in claim 2, and further comprising the amino acid substitution E(1202)G.
- 4. The HCV polynucleotide according to claim 2, wherein said substitution is a G2042C or a G2042R mutation.
- 5. The HCV polynucleotide according to claim 2, wherein said substitution is selected from the group consisting of: K(1691)R, and G(2042)C.
- 6. The HCV polynucleotide according to claim 2, wherein said substitution is selected from the group consisting of: R(1135)K, S(1560)G, K(1691)R, T(1993)A, G(2042)C, and P(2166)L.
- 7. The HCV polynucleotide according to claim 2, wherein said substitution is selected from the group consisting of: R(1135)K, S(1560)G, K(1691)R, T(1993)A, G(2042)C, L(2155)P, and P(2166)L.
- 8. The HCV polynucleotide according to claim 2, wherein said substitution is selected from the group consisting of: E(1202)G, I(1984)V, G(2042)C, and M(2992)T.
- 9. The HCV polynucleotide according to claim 2, wherein said substitution is selected from the group consisting of: S(1148)G, E(1202)G, L(1701)F, G(2042)R, and S(2404)P.
- 10. A HCV polynucleotide molecule comprising:
(a) a 5′-NTR region; (b) a HCV polyprotein region coding for an HCV polyprotein comprising one or more amino acid substitutions selected from the group consisting of: R(1135)K, S(1148)G, S(1560)G, K(1691)R, L(1701)F, l(1984)V, T(1993)A, G(2042)C, G(2042)R, S(2404)P, L(2155)P, P(2166)L, and M(2992)T, and (c) a 3′-NTR region.
- 11. The HCV self-replicating polynucleotide encoding a polyprotein comprising any one of the amino acid substitutions according to claim 10, further comprising the amino acid substitution E(1202)G.
- 12. The polynucleotide according to claim 10, wherein said substitution is a G2042C or a G2042R mutation.
- 13. The HCV polynucleotide according to claim 10, wherein said substitution is selected from the group consisting of: K(1691)R, and G(2042)C.
- 14. The HCV polynucleotide according to claim 10, wherein said substitution is selected from the group consisting of: R(1135)K, S(1560)G, K(1691)R, T(1993)A, G(2042)C, and P(2166)L.
- 15. The HCV polynucleotide according to claim 10, wherein said substitution is selected from the group consisting of: ,R(1135)K, S(1560)G, K(1691)R, T(1993)A, G(2042)C, L(2155)P, and P(2166)L.
- 16. The HCV polynucleotide according to claim 10, wherein said substitution is selected from the group consisting of: E(1202)G, 1(1984)V, G(2042)C, and M(2992)T.
- 17. The HCV polynucleotide according to claim 10, wherein said substitution is selected from the group consisting of: S(1148)G, E(1202)G, L(1701)F, G(2042)R, and S(2404)P.
RELATED APPLICATIONS
[0001] This application is a divisional of U.S. Application Serial No. 10/029,907, filed Dec. 21, 2001, which claims, as does the present application priority to U.S. Provisional Application Serial No. 60/257,857 filed on Dec. 22, 2000, the disclosures of all of which are incorporated by reference in their entirety.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60257857 |
Dec 2000 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
10029907 |
Dec 2001 |
US |
Child |
10309561 |
Dec 2002 |
US |