Patent Application
20070245812
This invention relates to semen analysis.
According to WHO statistics, 8-10% of all married couples consult medical professionals after failing to conceive. Over 40 million couples are currently being treated for infertility. Among these infertile couples, it is estimated that the infertility in 40% of the couples is due to male originating causes, and another 20% is due to combined male and female originating causes. Semen analysis is a major technique in evaluating male originating causes.
Standard semen analysis protocol involves the determination of at least three major semen parameters:
1. total sperm concentration (TSC);
2. percentage of motile sperm; and
3. percentage of normal sperm morphologies.
For all practical purposes, semen analysis, a key factor in human male fertility medicine, has not changed since the 1930's and is still done today by microscopic inspection. In fact, it is one of the very few remaining in vitro, body fluid analysis still performed almost solely via manual methods.
This manual methodology involves carefully observing the sperm cells, counting them to determine their concentration, classifying their motility, identifying their morphology, etc. This work requires high expertise, is very labor intensive and if done according to standard protocols, takes at least an hour per test.
Manual assessments are known to be quite inaccurate due to numerous sources of error. The main sources of error are:
Subjectivity of the observer.
The varying criteria used in the different labs and by different observers.
The large statistical errors due to the limited number of sperm analyzed.
The WHO manual (WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th edition, Cambridge University Press, 1999) recommends observing not less than 200 sperm and classifying the morphology and motility of each. This itself is an error introducing procedure due to the tediousness and time consuming nature of the task. In practice, 50 to 100 sperm cells at most are analyzed. Even if the observer introduces no errors, the statistical error alone reaches tens of percentages.
As a result of the above methodology, semen analysis test results are globally recognized to be highly subjective, inaccurate and poorly reproducible. Inter lab and inter technician variations are of such proportions that this issue is of major concern in male fertility medicine and the unresolved subject of discussion in the vast majority of symposiums, congresses and conventions on the subject.
In order to overcome these difficulties, medical instrumentation companies have introduced dedicated computerized systems based on image analysis (CASA—Computer Assisted Semen Analyzers). These systems require an extremely high quality image because all their results are based on image processing. Although these systems have attempted to replace manual analysis and establish industry accepted standards, they have not succeeded in either of these objectives.
The first objective could not be achieved because analysis results continue to be dependent on manual settings and on the different makes of equipment. Replacing routine manual analysis is totally impracticable because the systems are extremely expensive, complex and difficult to use. The fact is that such systems are generally not found in routine semen analysis but have rather established their niche almost solely in research centers, university hospitals and occasionally in highly specialized fertility centers.
An additional approach for semen measurements is described in U.S. Pat. Nos. 4,176,953 and 4,197,450, whose entire contents are incorporated herein. These patents describe a method for measuring sperm motility using electro-optical means and an analog signal analyzer. A suspension of sperm cells is continuously examined in a predetermined field in order to detect variations in optical density by the motion of the sperm. An amplitude-modulated analog electrical signal is generated in response to the variations, and the peaks and valleys of this signal are counted over a predetermined time period to provide an abstract parameter termed Sperm Motility Index (SMI). This parameter is related to motility and gives readings which are proportional to the number of motile cells multiplied by their respective velocity.
An automatic sperm analyzer called the Sperm Quality Analyzer (SQA), which provides the SMI parameter, has been on the market for a number of years. The analyzer is used in the following manner: a sperm specimen is taken up by a disposable chamber which has a rubber bulb at one end to aspirate the sample, and a thin measuring compartment at the other end. After aspirating the sample, the measuring compartment is inserted into the SQA and the SMI of the sample is automatically determined. The SMI parameter, although useful in some applications, was not significantly accepted by the medical community as a viable alternative to the conventional microscopic semen measurements.
It is common knowledge that in some fields of veterinary fertility analysis, total sperm concentration (TSC), is evaluated by measuring optical turbidity of the specimen. The physical principle behind this approach is that sperm cells are more opaque than the surrounding seminal plasma, and absorption of a light beam by the specimen is therefore proportional to the TSC.
For example, U.S. Pat. No. 4,632,562 discloses a method of measuring sperm density by measuring the optical absorbance of a sperm containing sample and relating the absorbance output signal to the density by using at least three summing channels. The disclosed method is intended for use in artificial insemination in the cattle breeding industry, and measures the optical absorbance in the range of 400-700 nm.
This technology however, has not and could not be adopted for human use for the following reasons:
(1) Human sperm concentrations in the normal range (and even in higher than normal cases), are more than an order of magnitude lower than in most of their veterinary counterparts—where this technology has been adopted.
(2) Human cases are treated even when sperm concentrations are far below their normal levels. This of course is not the case for animals. Infertile animals are normally culled—in any case, they are not treated for infertility.
(3) TSC in humans is a parameter, which in itself, is totally insufficient for fertility investigations, and microscopic analysis is in any case required for all the other data in the standard semen analysis protocol. To a large degree, this also holds for veterinary applications. This fact made optical absorption measurements superfluous, and no real effort has been invested in this field.
There is thus a need for a simple, objective technique for measuring TSC in human semen.
According to the WHO manual, sperm motility assessment (considered by most to be the most important single semen parameter) can be carried out manually using a grid system under the microscope or, alternatively, by use of CASA.
CASA provides some advantages over manual methods. However, accuracy and provision of quantitative data are totally dependent on precise semen preparation techniques and instrument settings. These factors (high expertise and sophisticated environment) along with the prohibitive cost of such instrumentation, rule out for all practical purposes their application for routine semen analysis.
U.S. Pat. No. 4,896,966 discloses a motility scanner for characterizing the motion of sperm, bacteria and particles in fluid. The scanner comprises an optical system including a collimating lens, condensing lens, imaging lens and a pair of reflecting elements, a source of illumination, radiation sensing means, signal processing means, and display means. The imaging lens has a useful depth of field at its object plane of at least about 0.2 mm.
It is an object of the present invention to provide a method for measuring TSC.
It is a further object of the invention to provide a method for determining the motile sperm concentration (MSC) and % motility.
It is a still further object of the invention to provide a sampling device for use in the determination of semen parameters.
It is another object of the invention to provide a system for the determination of semen parameters.
In a first aspect of the invention, there is provided a method for measuring the total sperm concentration (TSC) in a sample. The method comprises (i) placing the sample in a transparent container between a synchronically pulsed light source and a photodetector; and (ii) measuring the optical absorbance of the sample in the range of 800-1000 nm, the TSC of the sample being proportional to the absorbance.
The method of the invention provides an objective measurement of TSC which is not dependent on image analysis, and which can measure human TSC. However, the method may also be used to measure animal TSC.
In a second aspect of the invention, there is provided a sampling device for use in optically analyzing a biological fluid comprising:
In a preferred embodiment, the biological fluid is semen, most preferably human semen. The device serves both as a sampler and dual test chamber, enabling simultaneous testing of TSC and MSC. No dilution is required for any of the measurements. This not only saves labor but also eliminates a significant source of errors—namely, dilution inaccuracy.
The device also enables (when required) built-in visualizations of the specimen without transferring it to a separate viewing chamber. The thick chamber is also referred to as an optical densitometer.
In a third aspect of the invention, there is provided a method for measuring motile sperm concentration (MSC) in a semen sample comprising:
It has now been discovered that analysis of waveforms of the analog signals derived from a light beam which traverses a semen sample can provide an indication of the MSC. Using appropriately selected criteria, excellent correlation was found to exist between the averaged area covered by the waveform and the MSC. The MSC of a sperm sample is obtained in accordance with the invention by analyzing optical properties of the sample, which vary over time due to the motility of the sperm. This is in fact, the average signal amplitude in the relevant portions of the waveform, as will be described in more detail below.
In a fourth aspect of the invention, there is provided a method of determining the average velocity (AV) of sperm cells comprising:
Reference is made here to U.S. Pat. No. 4,176,953 issued Dec. 4, 1979, and which has been implemented in various versions of Sperm Quality Analyzers produced by Medical Electronic Systems, Israel. This patent, when applied to semen analysis, provides a parameter called SMI (Sperm Motility Index). As disclosed in the above patent and proven in numerous supporting studies, SMI is a function of both the concentration of motile cells (what is referred to as MSC) and their average velocity (AV). For the sake of simplification, we can say that SMI is a function of MSCxAV, or AV is a function of SMI/MSC. The average velocity of a sperm sample can provide an indication of the quality of the motility of the sperm.
Not withstanding that which is stated above, SMI as a function of MSC and AV is more complex than a direct multiplication. After observing, analyzing and measuring over a hundred semen samples, the correct inter-relationship (formula) between them has been developed. In general terms, the formula for extracting the average velocity can be defined as: AV=f(SMI/MSC), “f” being a polynomial of the third degree. Working with f(x)=1/1000x3+1/10x2+0.89x, provided a correlation factor of r=0.82.
It should be noted that most semen analysis protocols require data on the % of sperm having progressive motility rather than their average velocity. Progressive motility is defined as those sperm having an average velocity of 5 microns/second or more. This parameter too, can readily be extracted from the average velocity if a normal spread of velocities is assumed around the average. Even in cases where the velocity spread is not normal, the error in calculating the % of progressively motile sperm is not significantly affected. Moreover, when different minimal velocities are defined as progressively motile, this varying threshold is readily entered into the calculation, thereby giving extra flexibility in providing this parameter. This is important when working in different diluting media, ambient temperatures or in fact different species in vet measurements.
In a fifth aspect of the invention, there is provided a system for analyzing sperm viability comprising:
The system of the invention combines the measurement of the two major sperm parameters TSC and MSC, with the traditional visualization of the sperm, thus enabling acquiring the third parameter—sperm morphology. In a preferred embodiment, TSC and/or MSC are determined according to the methods of the invention. In another preferred embodiment, the system further comprises the sampling device of the invention.
It should be emphasized that there is a basic difference between the video visualization system used in the system of the invention and other sperm visualization systems (such as CASA). The other systems require extremely high quality images because all their results are built on image processing. In the present invention, on the other hand, visualization is used only as a complementary tool to view atypical or suspect cases, to add confidence to processed results, to identify specific pathologies and to enable manual sperm morphology assessment, when required.
In order to fulfill these tasks, the video visualization system used in the invention is designed as a compact, inexpensive subsystem, which although of limited use as a stand-alone, precisely fills a complementary role in the system of the invention. An additional important advantage of the visualization system as compared to microscopic procedures, is that pipetting, preparation of slides, dilutions and filling of hemocytometers is unnecessary. Use, together with the video visualization system, of the device of the invention, which doubles as a complete test chamber, obviates all of the above. These features, in effect permit and enable the use of the system of the invention in any small clinic or even office environment.
The video visualization system allows one to obtain the following supplementary information regarding the tested sample:
1. Measurement of Very Low Sperm Concentrations
Measuring TSC at very low concentrations (below 5 million sperm/ml) is inherently limited in accuracy. This is due to the fact that light absorption by factors other than sperm cells, may become relatively significant at these low levels. Light absorption may be due to seminal plasma variability or to the presence of cells other than sperm. The latter include WBCs (white blood cells indicating infections) and other immature or non-spermic cells from various sources, etc. Since according to the invention TSC is measured by optical absorption, without visualization there would be a possibility for ambiguity in the very low ranges due to the above mentioned considerations. When TSC is considered important in the low ranges, visualization enables differentiation between the different cells contributing to the light absorption. Since MSC is measured independently of light absorption, the % motility (MSC/TSC) can be calculated using the visually determined TSC parameter.
2. Identifying Foreign Cells in the Semen
The system is useful in identifying the presence of other cells which may have an effect on semen quality and/or assist in diagnosing patient ailment. For instance, leukocytes indicate infection, immature cells indicate a problem in spermatogenesis, agglutination may be due to a number of causes, etc.
3. Manual Sperm Morphology Assessment
Although the system of the invention automatically assesses the % of sperm with normal morphologies, it does so according to a given criteria (e.g. the WHO criteria). Regretfully this criteria is not universally accepted. Such universally accepted criteria do not yet exist, and are often a factor of application. For example, morphology criteria for IVF and ICSI applications are normally far stricter than in normal cases. Other international standards (such as strict or Krueger criteria) are also widely applied. Visualization allows the fertility practitioner to select his own criteria as well as to identify the specific defects present (head deformity, tail problem, etc.)
4. Vasectomy Validation and Azoospermia Diagnosis
In order to fully validate the outcome of vasectomy or to obtain a conclusive diagnosis of azoospermia, it is necessary to determine that there are absolutely no sperm in the semen under evaluation. This is generally not possible with the light absorption technology, because the concentrations that are to be measured can be very low. In this case, manual visualization is necessary in order to carefully scan large fields of view in search of individual sperm cells. The sperm visualization system used in the system of the invention is specifically tailored to optimally address these applications.
5. Hard Copy
The video visualization system enables “freezing” a given selected view (or a few views) which may then be printed and attached to the Semen Analysis Report. This is of great value for consultations and validation of treatment efficacy. A by-product of the freezing option is viewing the semen sample under static conditions. This strongly facilitates analysis and counting. In microscopic assessments, this can only be done by demobilizing (killing) the sperm prior to viewing. Even then, all dead sperm will end up in one layer, a condition which normally complicates analysis due to high concentration and sperm overlap in the said layer.
In order to understand the invention and to see how it may be carried out in practice, preferred embodiments will now be described, by way of non-limiting examples only, with reference to the accompanying drawings, in which:
As stated above, the automatic optical measurement of TSC in human semen samples as opposed to animal samples has been hampered in the past due to the low concentration of sperm cells. This, together with the high background electronic and optical noise due e.g. to seminal plasma variability has prevented the application of methods routinely used in veterinary fertility analysis. The method of the present invention comes to overcome these obstacles by combining the following features:
By using the method of the invention, the TSC of a sample may be determined as a function of the absorbance. Although the method of the invention is preferably used with samples of human semen or human sperm, it may also be used with animal semen and animal sperm, preferably after appropriate dilution.
An example of an optical system using one embodiment of the method of the invention is illustrated in
The beam path through the sample is preferrably vertical. The length of the beam path through the sample is generally between 5 and 15 mm, preferably 10 mm. The sample holder must be fully transparent to light waves in the near infra-red region of between 800 and 1000 nm. The plastic material from which the sample holder is made must be totally non toxic to sperm cells. A preferred material is polystyrene PG-79. The sample holder should preferably be designed to totally prevent penetration and forming of air bubbles in the sample, which interefere with the optical measurement.
By using the method of the invention, TSC detection levels down to appr. 2 million cells/ml. have been achieved. This level already indicates extreme semen pathology.
The optical viewing section 22 comprises a thin measuring chamber 28 and a thick measuring chamber 30. The thin chamber is used to measure MSC and/or for visualization, while the thick chamber is used to measure TSC. In this way, multiple parameters can be measured simultaneously using the same sampling device and sampling step.
The aspirating section 24 comprises a cylinder 32 and a plunger 34 slidingly inserted therein. These parts match each other and function as in a standard syringe. This section serves for the aspiration of the semen sample into the measuring chambers
The air exclusion section 26 comprises a separating valve 36 for separation of the measuring chambers from the cylinder volume after filling. The aspirator, thin measuring chamber, thick measuring chamber and air exclusion section are all in fluid communication.
An adapter 38 in the form of a rectangular rail extends along one side of the device 20 and serves for the correct sliding in and aligning of the device upon insertion into an optical instrument by which the sample is evaluated. It also provides the mechanical support and stability required for precision electro-optical measurements.
The parts of the device may be seen more clearly in
The thin measuring chamber serves for evaluation of sperm motility and may be positioned between a light source e.g. opposite the lower wall 42 and a photodetector e.g. opposite the upper wall 40. It will be understood that the light source and photodetector may also be positioned on the opposite sides of the chamber. A light beam is transmitted through the chamber containing a semen sample. The detector on the other side of the chamber registers optical density variations caused by moving sperm cells. The optical density variations are translated into an electrical signal by the photo-detector which is then routed to the electronic circuits to be filtered, digitized and processed so as to indicate the MSC. The thin measuring chamber may also be used with a video visualization system, as will be further explained below.
The thick measuring chamber 30 has an upper 46 and a lower 48 transparent wall through which an optical beam may pass. The distance between the walls is in the range of 0.5-3 cm, preferably 0.8-1.2 cm, most preferably approximately 1 cm. The approximate volume held by the thick compartment in the latter case would be approximately 0.5 ml.
This chamber serves for electro-optical absorption measurements of sperm concentration. A light beam, which may be the same or different from that of the thin chamber 28, is transmitted through the upper and lower walls of the chamber and detected by a photo-detector. The chamber volume should be completely filled with a sperm sample in order to avoid inaccuracies due to air bubbles. The attenuation of the light beam as it passes through the chamber is proportional to the sperm concentration. The light beam intensity is measured after passing through the chamber and translated to units of TSC by electronic means. The order of the chambers in the sampling device may be exchanged.
The cylinder 32 is in fluid communication with the two measuring chambers 28 & 30, so that by drawing the plunger 34, fluid is drawn into the chambers. This method of aspiration allows large sample volumes to be aspirated into the device. In order to prevent air bubbles from remaining in the measuring chambers, a separating valve 36 is interposed between the cylinder and the measuring chambers, and is in fluid communication with them. The valve is shown in detail in
When the valve is in the upper position, there is a connection between the measuring chambers and the aspirating cylinder. Pressing the valve down breaks that connection and ensures that no air remains in the measuring chambers where the samples are measured and no leakage will occur even when there is a temperature variation. This technique is equivalent to positive displacement since air is excluded from the measured fluid volumes (except at the anterior end 44). This design enables working with samples of virtually all viscosities, while at the same time preventing leakage and the penetration of air bubbles into the specimen volumes to be analyzed.
Although the means for excluding air from the measuring chambers has been exemplified by a separating valve, other means may also be used, such as a positive displacement pipette
All parts of the device may be manufactured from any material which is not toxic to the measured cells. Preferably, the material is relatively cheap, such as plastic materials, so that the device can be disposable. An example of a polymer which may be used to produce the device is polystyrene PG79. The separating valve, cylinder and piston may be made from polypropylene. The thin measuring compartment is by far the most toxi-sensitive part of the device due to the very high area to volume ratio of the seminal liquid in that section.
In order to aspirate a sample into the device 20, the tip 44 of the thin measuring compartment 28 is dipped approximately 5 mm deep into the semen sample, which is then aspirated into the device past the separating valve 36. Only app. 0.6 cc are required for a complete filling of the device. The separating valve is then pushed down, and the device may be inserted into an optical measuring apparatus.
As mentioned above, determination of the MSC according to the invention requires the generation of a voltage signal which is proportional to the MSC.
An optical capillary 100 having a rectangular cross-section is used to hold a semen sample 102. The capillary 100 is illuminated with an incident light beam 105 produced by a light source 110. The capillary 100 has an optical path of 300 μm through which the light beam 105 passes. After passing through the capillary, the scattered beam 106 is collimated by a round aperture 108 having a diameter of 70 μm. The collimated beam 107 impinges upon a photodetector 115. The photodetector 115 produces an analog voltage signal 120 proportional to the intensity of the beam 107. The analog signal varies in time due to the motility of the sperm in the semen sample 102, as shown for example in
A processor 135 is configured to carry out an analysis of data stored in the memory 130 in order to produce an analysis of the semen sample 102. The results of the analysis may be displayed on any display device such as a CRT screen 140 of a personal computer 145, or on an internal LCD screen 148 of the measuring device.
In step 200, the digital signal 128 of
In step 215, a waveform selection procedure is carried out to discard all waveforms due to artifacts such as from non-relevant cells, etc. A preferred embodiment of waveform selection with human sperm is to eliminate all waveforms not satisfying the following criteria:
Minimum height—10 millivolts.
Minimum width—37.5 milliseconds.
Maximum width—500 milliseconds.
Minimum rise/fall time—2.5 milliseconds.
The correct definition (and detection) of the beginning and end of sperm associated waveforms are defined as those where significant changes of waveform direction occur. The time difference between two such points defines the time width of a given wave. The manner of selection may be understood by way of example with reference to
In step 220 of
MSC=αa
where α is an empirically derived constant. In a preferred embodiment, the dependency of MSC on a may be described by a quadratic equation of the form:
MSC=Aa2+Ba
With reference to
MSC=0.0047a2+0.869a (I)
A good linear correlation was found to exist for small values of a. Using formula (I), the correlation factor (r) for fresh sperm over the entire range was >0.98.
Analysis of treated semen samples with varying viscosity was also performed using thawed samples, washed sperm, diluted samples (both in 3% Sodium Citrate and Test Yolk buffer) as well as with samples containing up to 20% glycerol having artificially raised viscosity. It was found that varying sample viscosity (and therefore sperm velocity), did not significantly affect the correlation between MSC and average signal (“r” in all case remained above 0.96).
Using centrifugal enrichment techniques, a very wide range of motile human sperm concentrations were measured (up to 250 M/ml). No significant saturation was found. The slight non-linearity at the highest ranges is easily corrected by a simple second-degree polynomial correction—given above.
Analysis of bovine semen was also carried out and correlation factors between bovine MSC and identically averaged signals (same methodology as for humans) provided similarly excellent results. It is to be noted however, that bovine semen has to be diluted prior to measurements. This is due to their MSC being typically an order of magnitude above that of human.
As explained above, the average velocity is a function of SMI and MSC. With reference to
In step 250 the results are displayed on the display device 145 or 148 (
One embodiment of a video visualization subsystem (VVS) which may be used with the analyzing system of the invention is illustrated in
In a preferred embodiment, the VVS is built around the sampling device of the invention, and particularly the thin measuring compartment. The object of this feature is that no extra preparations will be necessary to incorporate this function to the normal testing procedure. One simply takes the semen filled device on which the automated test is performed and inserts it—as such, into the viewing port. However, the VVS is not limited to use with the sampling device of the invention, and may be used with standard laboratory slides or smears.
The front end of the VVS is similar to that of the microscope. Two objective lenses are selectable for optimizing magnification and field of view, according to the application (×20 or ×40). However, instead of the eyepieces of the microscope, the image from the objective is conveyed to a miniature CCD video camera. The size of the CCD (diagonal) is 6 mm. The viewing screen is a 100 mm LCD. This provides a video amplification of app. 17. This in effect gives a potential overall amplification of 340 or 680. Although amplification factors of only 200 and 400 are required, this set up is selected so that the above amplification could be reached in a much smaller construction. This is desirable e.g. for a compact and robust desk-top unit (decreasing the specified image distance decreases the amplification to what is required).
The lenses and their magnification set-up may be selected so that the “Working distance” (from object to lenses) can be varied to enable scanning throughout the whole depth of the thin measuring compartment (e.g. 300 microns). This is opposed to normal microscopic viewing which does not require such scanning, because the object is normally enclosed in a slide which is just 20 microns deep and the whole depth can be viewed without scanning or refocusing.
As mentioned above, an overall amplification factor of 200 or 400 may be selected. An amplification of 400 will be the choice when it is necessary to identify non-spermic cells (white blood cells, round cells, etc.), as well as to investigate and evaluate various morphological pathologies of sperm cells (agglutinations, immature cells, sperm head or tail defects, etc.). An amplification of 200 will be preferable for cell counting—irrespective of whether they are sperm or others. The lower amplification provides a larger field of view (4 times larger) and thereby improved counting statistics. The possibility of freezing images greatly enhances both applications.
In order to facilitate cell counts and acquire a truly quantitative result using the VVS, in a preferred embodiment a calibrated grid may be charted directly on the LCD viewing screen. The grid comprises 2 cm squares which are equivalent to a pre-amplification size of 0.1 mm in the semen filled measuring compartment (amplification factor of 200). This approach precludes the very difficult task of precisely charting a minute grid on the measuring compartment itself. The latter expensive solution is incorporated in the Mackler Counting Chamber as well as some other hemacytometers—precluding their use as disposables. In the present invention this is unnecessary and the VVS allows the grid to be a part of the viewing screen.
The VVS may be useful in the following applications:
| Number | Date | Country | |
|---|---|---|---|
| Parent | 10478251 | Nov 2003 | US |
| Child | 11812558 | Jun 2007 | US |
