The present invention relates to a sensing device that uses a resonator, e.g. a quartz resonator, which has an adsorbing layer for adsorbing an object to be detected on the surface of the resonator and changes the natural frequency thereof by adsorbing of the object, and senses the object by detecting the variation in the natural frequency of the sensor resonator.
The necessity to know the concentration of various pollutants in a river or soil has been increasing for the purpose of maintaining environmental preservation. There are some pollutants which have very strong toxicity to the human body even with a very small quantity. Consequently, the establishment of measurement technology for the pollutants in a very small quantity has been expected. Dioxin is one of the pollutants on which much attention has been recently focused and methods of using a gas chromatography mass spectrometer and Enzyme-Linked Immuno Sorbent Assay (ELISA) are known as a method of measuring dioxin. Although it is possible to perform highly accurate microanalysis of the order of 10−22 g/ml by gas chromatography mass spectrometry, the method has disadvantages in that the measurement device is very costly, which makes the cost of analysis unexpectedly expensive, and furthermore, it takes a long time for results to be known. In the meantime, the ELISA method is cheaper in price for the equipment and analysis, and less time consuming, compared with the gas chromatography mass spectrometry, but it has the disadvantage of low accuracy of the order of 10−9 g/ml.
Consequently, the present inventor pays attention to a quartz sensor which uses a quartz resonator as a measurement device for pollutants such as dioxin because once an object to be detected adheres to the quartz resonator, the natural frequency thereof varies according to the amount of adhesion. Recently, the development of antibodies that chemically bonds with only certain specific molecules has become popular, and it has become possible to perform analysis in various fields by forming an adsorbing layer using an antibody that causes an antibody-antigenic reaction against a sample on the surface of the quartz resonator (or to be more exact, on the electrode surface) in advance.
Meanwhile, in a method to measure a pollutant in a solution or an antibody in blood using a quartz resonator, cleaning is necessary for repeated use of a sensor portion, it sometimes takes a long time for the antibody-antigenic reaction, and furthermore, a static evaluation is conducted by obtaining distribution data through measurement of many samples. Therefore, an efficient method is desired. Under these circumstances, the present inventor attempts to measure simultaneously using many quartz sensors. The technology of using many quartz sensors is described in Patent Document 1 which discloses technology to measure the type of corrosive substances in the air and the concentration thereof.
When measurement is conducted using many quartz sensors, there exists the following disadvantage. That is, although the oscillation frequency of a quartz resonator is determined by the design value, it is practically impossible for actual products to make each oscillation frequency precisely identical to one another. It is also practically impossible to form adsorbing layers with absolutely the same size and thickness on the electrode surfaces. Therefore, there is a frequency difference between each though very small. Accordingly, when the distance between respective quartz sensors is small, in other words, when the distance between the quartz sensors adjacent each other, these quartz sensors are spatially connected so that the oscillation frequencies become unstable affected by mutual frequencies. This phenomenon resembles to that when antennas are brought closer together, the oscillation frequency becomes unstable, which makes it impossible to measure the frequency accurately. Therefore, it becomes difficult to detect the object, in other words, it becomes difficult to measure the exact concentration of the object or to detect the presence or absence of the object in very small quantities with a high degree of accuracy.
Though it is possible to avoid such phenomena by making the distance between respective quartz sensors greater, this can result in the device being too large. The present inventor is attempting to measure the concentration of the object, for instance, in 8 sample solutions prepared by diluting the sample solution containing the object with eight different degrees of dilution, using eight quartz sensors having the same oscillation frequency design value, and to evaluate their concentration statistically. When a large number of the quartz sensors are used, the device cannot be minimized sufficiently and it departs from the market demand unless they are arranged in a closely packed fashion.
Japanese Patent Application Laid-open No. 2001-99777: FIGS. 1 and 24.
The present invention has been achieved under such a circumstance, and an object of the present invention is to provide a technology to conduct detection of an object to be detected (measurement of concentration or detection of the presence or absence of the object to be detected) in a stable fashion in a sensing device using a plurality of sensor resonators including a piezoelectric plate such as a quartz board which forms an adsorbing layer to adsorb an object to be detected on the surface thereof and changes the natural frequency thereof by adsorption of the object.
The sensing device of the present invention which uses a sensor resonator including a piezoelectric plate forming an adsorbing layer to adsorb an object to be detected on the surface thereof and changes the natural frequency thereof by adsorption of the object, and detects the object by variation of the natural frequency of the sensor resonator, includes:
a plurality of sensor resonators;
a plurality of the oscillation circuits to oscillate a plurality of the sensor resonators respectively;
a measuring unit provided for these plural oscillation circuits in common to measure the signal relating to the frequency of the oscillation circuit;
a signal switchover unit to perform switchover connection of the plural oscillation circuits to the measuring unit in turn;
a load switchover unit installed between each of the plural oscillation circuits and the signal switchover unit to switchover the load on the output side of each oscillation circuit between a first value and a second value in order to forcibly separate the respective oscillation frequencies of the oscillation circuits connected to the measuring unit from the oscillation circuit not connected to the measuring unit; and
a switchover controller which outputs a control signal to the signal switchover unit so that each of the plural oscillation circuits is connected to the measuring unit in turn, and outputs a control signal to the load switchover unit so that the load on the output side of the oscillation circuit connected to the measuring unit is the first value and the load on the output side of the oscillation circuit selected from among the oscillation circuits not connected to the measuring unit is the second value.
The load switchover unit includes: for instance, a plurality of terminal loads having the second values respectively provided at the terminals of the plural oscillation circuits; and a switch provided between each of the plural oscillation circuits and the signal switchover units to switchover and connect the output side of each oscillation circuit between the signal switchover unit and the terminal load having the second value, and
the switchover controller outputs a control signal to the switch so that the oscillation circuits selected from among the oscillation circuits not connected to the measuring unit respectively connect to the terminal load. More concretely, it is possible to cite a formation in that buffer circuits are provided between the respective oscillation circuits and the signal switchover units and the switch is provided between each oscillation circuit and each buffer circuit. The oscillation circuits selected from among the oscillation circuits not connected to the measuring unit can be all of the oscillation circuits not connected to the measuring unit or can be a part of the oscillation circuits. The sensing device of the present invention includes a sensor unit including a holding member provided on every sensor resonator to hold each sensor resonator and connection terminals of the sensor resonator electrodes; and a measuring device main unit, detachably provided to the connection terminal of the sensor unit and at the same time includes each oscillation circuit and the measuring unit.
According to the present invention, when a plurality of sensor resonators, for instance, quartz resonators of which natural frequency changes due to adsorption of an object to be detected, a common measuring unit to measure the change of the frequency is provided for each oscillation circuit corresponding to each sensor resonator so as to perform switchover connection in turn, and is structured in a manner that the terminal loads of the oscillation circuit connected to the measuring unit are the first values, and the terminal loads of the oscillation circuits selected from among the oscillation circuit not connected to the measuring unit, for instance, the terminal loads of all oscillation circuits not connected to the measuring unit are the second values. Therefore, it is possible to avoid overlapping of mutual spectrum by forcibly separating the mutual oscillation frequencies of the sensor resonator from each other. Accordingly, it is possible to perform detection of the object (measurement of concentration and measurement of the presence or absence of the object to be detected) in a stable fashion. Furthermore, since the measuring unit is used in common for a plurality of sensor resonators, the circuit configuration is simple, so that the cost reduction can be realized.
Hereinafter, an embodiment of a sensing device relating to the present invention will be explained. Though the present embodiment has a principal portion in the rear circuit part of an oscillation circuit, the whole structure will be explained briefly first. The sensing device includes a quartz sensor 1 composed of a plurality of, for instance 8 pieces of, sensor units; and a measurement device main unit 100 to which the quartz sensor 1 is detachably installed, as shown in
The quartz resonator 24 includes electrodes 24a and 24a (electrodes on the back surface side cannot be seen) on both surfaces of, for instance a round shaped, quartz plate 20 as shown in
The measuring device main unit 100 includes an oscillation circuit for oscillating the quartz sensor 1 and a measuring unit for measuring the frequency of a frequency signal from the oscillation circuit. An inner circuit of the measuring device main unit 100 will be explained in reference to
The measuring unit 200 may be a means for measuring the frequency of, for instance, the oscillation circuit and determine the deviation based on the measurement, but may be a means for directly determining the deviation in frequency of the oscillation circuit as will be described later. In this embodiment, the measuring unit 200 includes an analogue/digital (A/D) converter 201 which converts a frequency signal (analogue signal) sent from anyone of the oscillation output units 31 to 38 into a digital signal and a measurement circuit unit 202 for measuring the frequency or directly measuring the deviation in frequency by processing the digital signal from the A/D converter 201.
Here, the structure of the oscillation output unit 31 on behalf of the oscillation output units 31 to 38 will be explained in reference to
Since the oscillation frequency and the output level are influenced by the load value on the output side. the oscillation circuit 51 is connected to the measuring unit 200 via the buffer circuit 53 lest the load value should fluctuate. The present invention utilizes that the oscillation frequency and the output level of the oscillation circuit 51 sensitively change according to the magnitude of the load on the output side as described above, and when the oscillation circuit 51 is not connected to the measuring unit 200, the output side of the oscillation circuit 51 is connected to the terminal load 54 by switchover of the switch 52, so as to be an oscillation frequency different from the oscillation frequency which is obtained when connected to the buffer circuit 53. That is, assuming that the load on the output side is the first value when the oscillation circuit 51 is connected to the buffer circuit 53, the load on the output side is the second value when it is switched over onto the terminal load 54 side. The second value is set to be a size that can avoid overlapping of mutual spectrum by forcibly separating the oscillation frequency of the oscillation circuit 51 in the channels connected to the measuring unit 200 among 8 channels from the oscillation frequency of the oscillation circuit 51 in the channels not connected to the measuring unit 200. In addition, the absolute amount of connection itself is also reduced by making the output level lower. More specifically, for instance, the value (the second value) of the terminal load 54 is set such that the oscillation frequency at the time when the switch 52 is switched over on the terminal load 54 side is shifted about several hundred ppm with regard to the oscillation frequency at the time when the switch 52 is switched over on the buffer circuit 53 side. Far more specifically, for instance, in the case of oscillation circuit of 31.1 MHz, it is set so as to be displaced by 25 kHz.
As described above, in order to avoid overlapping of spectrum for the output of mutual oscillation circuits 51, in other words, in order to avoid the phenomenon that the oscillation frequency becomes unstable influenced by mutual oscillation frequency, it is preferable that the displacement of oscillation frequency is as large as possible. However, if it is made too large, it takes a long time until the oscillation frequency returns to the original magnitude and gets stable when the channel switched off from the measuring unit 200 is connected to the measuring unit 200. Then, the channel switchover speed is reduced, which requires a long time for the measurement. Therefore, the amount of displacement is determined considering both situations.
Next, the function of the above embodiment will be explained. First, the quartz sensor 1 is plugged in the measuring device main unit 100, and the respective channels including the oscillation circuits 51 are connected to the measuring unit 200 by the signal switchover unit 4 in turn, for instance, in a state that the quartz sensor 1 is not filled. In the measuring unit 200, the oscillation frequency from each oscillation circuit 51 is taken in the measurement circuit unit 202 via the A/D converter 201 to determine each oscillation frequency (blank value) at this time, for instance. Note that in order to determine the blank value, pure water or other solutions may have been poured into the quartz sensor 1. Then, 8 kinds of the sample solution for measurement are prepared by changing the dilution ratio of the sample solution to be measured, which are poured into 8 quartz sensors 1. Each channel is connected to the measuring unit 200 by the signal switchover unit 4 in turn to find each oscillation frequency, and the deviation in the oscillation frequency caused by pouring the sample solutions for each channel are determined. It should be noted that in this case, the deviation caused by coming into contact of the quartz resonator 24 with the liquid is included in addition to that caused by adsorption of the object onto the adsorbing layer of the quartz resonator 24. Therefore, it is possible to perform the measurement in such a manner that the amount of deviation in oscillation frequency is determined in advance at the time of pouring, for instance, pure water into the quartz sensor 1 and the value obtained by canceling the amount of deviation is handled as the measurement value for the variation in frequency. It is also possible to conduct the measurement operation in such a manner that the oscillation frequency is measured after pouring pure water before pouring the sample solution into the quartz sensor 1 and then the sample solution is poured into the quartz sensor 1 instead of pure water.
Synchronizing with the operation of the above-described channel switchover connection, switchover of the oscillation circuit 51 by the switch 52 serving as a load switchover unit is performed. That is, when the oscillation output unit 31 is connected to the measuring unit 200 by switchover of the switches SW1, SW5 and SW7 shown in
According to the above embodiment, the common measuring units 200 to measure the variation in frequency are provided to each oscillation circuit 51 corresponding to each quartz sensor 1 so as to switchover and connect in turn. As for the oscillation circuits 51 excepting the oscillation circuits 51 connected to the measuring unit 200 by the switches 52 serving as the load switchover unit, the load value on the output side of the oscillation circuit 51 included (relating to the measurement) in the channels connected to the measuring unit 200 and the load value on the output side of the standby oscillation circuit 51 are made different from each other, so that the respective oscillating frequencies are forcibly separated from each other. Therefore, the overlap of mutual spectrum can be avoided. Accordingly, the situation that the oscillation frequencies become unstable because that both oscillation frequencies pull each other can be avoided, so that sensing of the object (measurement of concentration and detection of the presence or absence of the object) can be conducted in a stable fashion.
It should be noted that in this embodiment, the output sides of the oscillation circuits 51 are connected to the terminal loads 54 for all channels except channels connected to the measuring unit 200. However, as for channels which are separated from the quartz sensor 1 relating to the measurement in some extent and have no fear of occurrence of spatial connection, they may be not connected on the terminal load 54 side. and switchover control of the switch 52 may be conducted so as to connect on the terminal load 54 side, for only channels containing the quartz sensors 1 which are, for instance, adjacent to the quartz sensor 1 relating to the measurement.
In the description above, it is possible that the measuring unit 200 measures an oscillation frequency in each quartz sensor 1 and counts, for instance, the blank value and the oscillation frequency when the sample solution is poured, and these count values are stored. Then, the difference between both, in other words, the variation of the frequency is determined, or based on the variation, the concentration of an object to be detected is determined using a calibration curve obtained previously and display the concentration. Furthermore, a threshold value for the variation of the frequency is determined in advance, so that the presence or absence of an object is determined. The measuring unit 200 is not limited to that counts the oscillation frequency of the oscillation circuit 51, but it may be that directly determines the variation of the oscillation frequency.
As a method of directly determining the variation of an oscillation frequency, it is possible to cite the following example. An frequency signal from the oscillation circuit 51 is sampled by the reference clock signal, the sampled value is converted into a digital signal by an A/D converter, and orthogonal detection by the digital signal is conducted for the frequency signal corresponding to the digital signal. Then, the real number portion and the imaginary number portion are taken out when displaying the rotation vector rotating at the speed corresponding to the variation of the frequency in the frequency signal in complex notation, and at the same time, the deviation in frequency of frequency signal is determined based on each time series data of the real number portion and the imaginary number portion.
An example of carrying out the above-described method will be described. In
In general, when a sine wave signal having a frequency of f1 is sampled by a clock signal having a frequency of fs, the frequency f2 as a result of the capturing is expressed by Equation (1), where mod (,) indicates a modulo function.
f2=|mod(f1+fs/2, fs)−fs/2| (1)
In the result of this capturing, since the frequency of an n-power harmonic is expressed by n×(frequency of fundamental) in regard to the fundamental, if this is put as f2 and substituted into the above Equation (1), it is possible to calculate the frequency of the captured harmonic. By using this calculation, it is possible to set the frequency fc of the high frequency signal from the oscillation circuit 51 and the sampling frequency (frequency of clock signal) fs so as not to overlap the frequency of the fundamental and that of the harmonic. For instance, fc is set to be 11 MHz, fs is 12 MHz. In this case, the fundamental of a frequency signal defined by the output signal which is a digital signal from the A/D converter 61 is a sine wave having a frequency of 1 MHz. Note that if fc/fs is set to be 11/12, though the frequency of the fundamental and the frequency of the harmonic do not overlap with each other, fc/fs is not limited to this value.
At the rear part of the A/D converter 61, a carrier remove 7 and a lowpass filter 8 are arranged in this order. The carrier remove 7 and the lowpass filter 8 correspond to a means for capturing a rotation vector relating to the variation in frequency of tie sine wave signal, in more detail, a means for capturing the real number portion and the imaginary portion when the rotation vector is displayed in complex notation, when the sine wave signal having a frequency of 1 MHz defined by a digital signal from the A/D converter 61 is A cos(ω0t+θ).
In other words, the carrier remove 7 includes a multiplier 71a multiplying cos(ω0t) to the sine wave signal and a multiplier 71b multiplying −sin(ω0t) to the sine wave signal as shown in
A cos(ω0t+θ)·cos(ω0t)=½·A cos θ+½{cos(2ω0t)·cos θ+sin(2ω0t)·sin θ} (2)
A cos(ω0t+θ)·−sin(ω0t)=½·A sin θ−½{sin(2ω0t)·cos θ+cos(2ω0t)·sin θ} (3)
Accordingly, by letting the output of the multiplier 71a and the output of the multiplier 71b pass through the low pass filters 72a and 72b respectively, ½·A cos θ and ½·A sin θ are captured from the low pass filter 72, because the frequency signal 2ω0t is removed. Note that the low pass filter 72 is described as to be composed of low pass filters 72a and 72b. Actual digital processing in the low pass filter 72 calculates the moving average of successive plural data, for instance 6 pieces of data, among the time series data outputted from the carrier remove 71.
When the frequency of a sine wave signal expressed by A cos(ω0t+θ) is varied, A cos(ω0t+θ) becomes A cos(ω0t+θ+ω1t). Accordingly, ½·A cos θ becomes ½·A cos(θ+ω1t), and ½·A sin θ becomes ½·A sin(θ+ω1t). In other words, the output obtained from the low pass filter 72 is the signal corresponding to the variation of a frequency (ω1t) of the sine wave signal [A cos(ω0t+θ)], in more detail, the real number portion (I) and the imaginary number portion (Q) when a vector rotating at a speed of the variation in frequency is displayed in complex notation.
Number | Date | Country | Kind |
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2004-381432 | Dec 2004 | JP | national |
Filing Document | Filing Date | Country | Kind | 371c Date |
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PCT/JP2005/024272 | 12/28/2005 | WO | 00 | 8/27/2007 |