The Sequence Listing for this application is labeled “PRX-103US_SequenceListing” which was created on Mar. 23, 2020 and is 13.6 KB. The entire content of the sequence listing is incorporated herein by reference in its entirety.
The invention relates to a sensitive method for detecting G protein coupled receptor-associated sorting 1 (GASP-1), GASP-1 microvesicles, and GASP-1 exosomes at a very low concentration in a sample and applications thereof.
One way for detecting cancer is to use cancer biomarkers present on microvesicles or exosomes. Exosomes are smaller luminal vesicles (30-100 nm in diameter) originating from intracellular endosomes. Microvesicles are small membrane vesicles (0.1-1 μm in diameter) released from cell membrane surface. In additional to blood, microvesicles and exosomes are present in other biological fluids, including saliva, urine, and cerebrospinal fluid.
Analysis of microvesicle or exosomal levels of circulating cancer biomarkers has been shown promising in cancer detection. Although several potential cancer biomarkers have been identified through proteomic analyses of cancer-derived microvesicles or exosomes from various types of cancers, none has been found suitable for early cancer detection.
One of the long-term goals for cancer research has been to discover a universal blood test that can detect the presence of cancer cells in an individual long before showing any cancer symptom. To be useful, new blood tests for cancer detection must have very high specificity; otherwise, too many healthy individuals will receive false positive test results, leading to unnecessary follow-up procedures and anxiety. Ideally, for a cancer test to be of practical use, it is desirable to have sensitivity of 90% or higher so that the false positive rate would be no more than 10%. Despite many efforts, no cancer biomarker has been reported to reach such high sensitivity and specificity for early cancer detection.
G protein coupled receptor-associated sorting 1 (GASP-1) is a useful biomarker for cancer detection as well as assessment of cancer progression and treatment, but has not been shown useful for early cancer detection, especially in symptom-free individuals.
There remains a need for a highly sensitive and highly specific detection method to use GASP-1 as a biomarker for early cancer detection.
The present invention provides a method of detection G coupled receptor-associated sorting 1 (GASP-1) or a fragment thereof in a sample and a related kit. The detection method is surprisingly sensitive and specific.
A method of detecting G protein coupled receptor-associated sorting 1 (GASP-1) or a fragment thereof in a sample is provided. The method comprises exposing a surface to a sample comprising GASP-1 or a fragment thereof. A coating agent is immobilized to the surface and is selected from the group consisting of a first GASP-1 fragment, a conjugate of a protein and the first GASP-1 fragment, a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, and a combination thereof. The first and second GASP-1 fragments are different. The method also comprises adding to the surface an anti-GASP-1 detection antibody against the first GASP-1 fragment. The anti-GASP-1 detection antibody has a concentration of 0.01-1 ng/ml. The anti-GASP-1 detection antibody is immobilized to the surface via the coating agent, directly or indirectly. The method further comprises measuring the amount of the anti-GASP-1 detection antibody immobilized to the surface and determining the presence of the GASP-1 or a fragment thereof in the sample based on the amount of the anti-GASP-1 detection antibody immobilized to the surface.
The method may further comprise quantifying the concentration of the GASP-1 or a fragment thereof in the sample based on the amount of the anti-GASP-1 detection antibody immobilized to the surface and a standard curve of GASP-1 generated for the surface using the anti-GASP-1 detection antibody.
The method may have a detection sensitivity of 100%. The method may have a detection specificity of 100%. The protein may be bovine serum albumin (BSA). The anti-GASP-1 detection antibody may have a titer of over 1:200,000.
The first GASP-1 fragment may consist of an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). In one embodiment, the first GASP-1 fragment consists of the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2).
The second GASP-1 fragment may consist of an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). In one embodiment, the second GASP-1 fragment consists of the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2).
The method of claim 1, wherein the microvesicle or exosomal surface biomarker may be selected from the group consisting of CD9, CD63, CD81 and a combination thereof.
The sample may comprise 0.01-0.02 ng/ml of the GASP-1 or a fragment thereof.
In some embodiments, the GASP-1 or a fragment thereof in the sample is from a biological fluid of a subject. The method may further comprise determining the concentration of the GASP-1 or a fragment thereof in the biological fluid in the subject. The biological fluid may comprise 0.01-0.02 ng/ml of the GASP-1 or a fragment thereof. The subject may be free of a cancer symptom. The subject may have cancer. The cancer may be selected from the group consisting of breast cancer, bladder cancer, ovarian cancer, colon cancer, endometrial carcinoma, esophagus squamous cell carcinoma, glioma, head cancer, neck cancer, hepatocellular carcinoma, infiltrating ductal breast carcinoma, larynx cancer, lung cancer, melanoma, mucinous cystadenocarcinoma of ovary, pancreatic cancer, prostate cancer, renal cell carcinoma, small bowel malignant stromal tumor, and stomach adenocarcinoma. The method may further comprise comparing the concentration of the GASP-1 or a fragment thereof in the biological fluid in the subject with a concentration of the GASP-1 or a fragment thereof in a control biological fluid from a symptom-free individual.
A first kit for detecting G protein coupled receptor-associated sorting 1 (GASP-1) or a fragment thereof in a sample is provided. The first kit comprises a surface and an anti-GASP-1 detection antibody against the first GASP-1 fragment. A coating agent is immobilized to the surface and is selected from the group consisting of a first GASP-1 fragment, a conjugate of a protein and the first GASP-1 fragment, a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, and a combination thereof. The first and second GASP-1 fragments are different. The anti-GASP-1 detection antibody has a concentration of 0.01-1 ng/ml.
A second kit for detecting G protein coupled receptor-associated sorting 1 (GASP-1) or a fragment thereof in a sample is provided. The second kit comprises a surface, a coating agent and an anti-GASP-1 detection antibody against the first GASP-1 fragment. The coating agent is selected from the group consisting of a first GASP-1 fragment, a conjugate of a protein and the first GASP-1 fragment, a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, and a combination thereof. The first and second GASP-1 fragments are different. The anti-GASP-1 detection antibody has a concentration of 0.01-1 ng/ml.
The present invention provides a highly sensitive and highly specific method of detecting G protein coupled receptor-associated sorting 1 (GASP-1) in a sample at a very low concentration and uses of this method for cancer detection, especially early cancer detection, and assessment of cancer progression and cancer treatment.
GASP-1 is a cytosolic protein, which is only minimally expressed in normal cells but become highly overexpressed in many cancers, including breast, brain, liver and lung cancers. In the cytosol, GASP-1 interacts with the C-terminal end of many G protein coupled receptors (GPCRs). Through its binding to different GPCRs, GASP-1 functions as a signal transduction silencer by directing the ligand-bound GPCRs via “sorting endosomes” to lysosomes for destruction. However, when GASP-1 is overexpressed, this sorting process is inhibited and the ligand-bound GPCRs together with GASP-1 stay in the endosomes (“recycling endosomes”). After multivesicular bodies, a subset of the recycling endosomes, fuse with plasma membrane, intraluminal vesicles are formed and their contents are released as exosomes. Some recycling endosomes may fuse directly with plasma membrane, and become part of the plasma membrane before being released as microvesicles.
The invention is based on the inventors' surprising discovery of GASP-1 expression in symptom-free individuals and circulating GASP-1 in cancer patients, as well as GASP-1 containing microvesicles and GASP-1 containing endosomes released from cancer cells. For detection of various cancers, the method of the present invention has been found to have unexpectedly high sensitivity of, for example, up to 100%, and unexpectedly high specificity of, for example, up to 100%. Developing a detection method capable of differentiating a cancer patient from a symptom-free individual has been a major goal of many oncologists for some time. Despite all efforts, this goal has not been achieved. The inventors have unexpectedly developed a detection method capable of differentiating cancer patients from symptom-free individuals with up to 100% sensitivity, up to 100% specificity, and an AUC number of up to 1.0. None of the reported cancer biomarkers has reached a perfect AUC number of 1.0.
To achieve complete discrimination of cancer patients from symptom-free individuals, the inventors have surprisingly discovered that it is desirable to use a very high titer affinity-purified anti-GASP-1 detection antibody, and, where the anti-GASP-1 detection antibody is biotinylated and detected by streptavidin-horseradish peroxidase (HRP), it is also desirable to dilute both the biotinylated anti-GASP-1 detection antibody solution and the streptavidin-HRP solution extensively. The term “titer” used herein refers to the lowest dilution ratio of a detection antibody to a dilution liquid (e.g., buffer) permitting specific binding of the antibody to its corresponding epitope, for example, the lowest dilution ratio of an anti-GASP-1 detection antibody to a dilution buffer permitting specific binding of the anti-GASP-1 detection antibody to a GASP-1 fragment. These adjustments not only decrease the background signal significantly but also amplify the positive signal. To ensure successful complete discrimination of cancer patients from symptom-free individuals, a detection antibody having a high titer before biotinylation is desirable. For example, an anti-GASP-1 detection antibody having a titer exceeding 1:200,000 may be used to detect GASP-1 or a fragment thereof according to the present invention.
The terms “symptom-free,” “normal” and “control” are used interchangeably and refer to an individual who has not shown any cancer symptom. The symptom-free individual may have been tested positive or negative with a cancer biomarker. The symptom-free individual may have cancer cells. The symptom-free individual may be predisposed to cancer.
The term “cancer patient” used herein refers an individual who has shown at least one cancer symptom. The cancer patient may have been tested positive or negative with a cancer biomarker. The cancer patient has cancer cells. The cancer patient may have received a cancer treatment.
The present invention provides a method of detecting G protein coupled receptor-associated sorting 1 (GASP-1) or its fragment in a sample. The method comprises exposing a surface to a sample comprising GASP-1 or its fragment; adding an anti-GASP-1 detection antibody to the surface; measuring the amount of the anti-GASP-1 detection antibody immobilized to the surface; and determining the presence of the GASP-1 or a fragment thereof in the sample based on the amount of the anti-GASP-1 antibody immobilized to the surface.
A coating agent may be immobilized to the surface. The coating agent may be a first GASP-1 fragment, a conjugate of a protein, for example, bovine serum albumin (BSA) or casein, and the first GASP-1 fragment (protein-GASP-1 conjugate), a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, or a combination thereof. The first and second GASP-1 fragments are different.
The anti-GASP-1 detection antibody may be immobilized to the surface via the coating agent, directly or indirectly. The anti-GASP-1 detection antibody may have a high concentration of, for example, 0.01-1 ng/ml or 0.01-0.1 ng/ml. The anti-GASP-1 detection antibody may be affinity purified, for example, purified by, for example, passing an antibody serum through an affinity column having a specific GASP-1 fragment attached to it, followed by elution of the bound antibody. The specific GASP-1 fragment may be selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID: 8). In one embodiment, the specific GASP-1 fragment consists of the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2). The anti-GASP-1 detection antibody may have a high titer, for example, over 1:200,000, 1:250,000, 1:300,000, 1:350,000, 1:400,000, 1:450,000 or 1:500,000, or from 1:200,000 to 1:500,000, from 1:200,000 to 1:400,000, or from 1:200,000 to 1:300,000.
The method may further comprise immobilizing the coating agent to a surface such that the surface used for exposing to the sample is obtained. Before the exposure, the coated surface may be blocked by a blocking agent, for example, a BSA solution, to block sites on the surface not covered by the coating agent. The coated and blocked surface may be washed before the exposure.
The method may further comprise quantifying the concentration of the GASP-1 or a fragment thereof in the sample based on the amount of the anti-GASP-1 detection antibody immobilized to the surface and a standard curve of GASP-1 generated for the surface using the anti-GASP-1 detection antibody. The GASP-1 standard curve may be generated using a series of solutions comprising GASP-1 at different concentrations. The method may further comprise generating the GASP-1 standard curve.
The coating agent may be a first GASP-1 fragment. The first GASP-1 fragment may be any fragment of GASP-1 (SEQ ID NO: 1), preferably any GASP-1 peptide known to be exposed on the surface of a cell, a microvesicle or an exosome. For example, the first GASP-1 fragment may comprise an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). The first GASP-1 fragment may consist of an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). In one embodiment, the first GASP-1 fragment comprises the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2). In another embodiment, the first GASP-1 fragment consists of the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2).
The coating agent may be a protein-GASP-1 conjugate of a protein and the first GASP-1 fragment. The protein may be BSA and the corresponding conjugate may be a BSA-GASP-1 conjugate. The protein may be casein, and the corresponding conjugate may be a casein-GASP-1 conjugate. The method may further comprise binding the anti-GASP-1 detection antibody specifically to the protein-GASP-1 conjugate, for example, the BSA-GASP-1 conjugate.
The coating agent may be a capture antibody against a microvesicle or exosomal surface biomarker. The GASP-1 or a fragment thereof may be on the surface of a microvesicle or an exosome in the sample. The method may further comprise binding the microvesicle or exosome specifically to the capture antibody, and/or binding the anti-GASP-1 detection antibody specifically to the GASP-1 or its fragment. The microvesicle or exosomal surface biomarker may be selected from the group consisting of CD9, CD63, CD81, and a combination thereof. The microvesicle or exosomal surface biomarker may be selected from other reported exosomal proteins such as Glypican-1 (GPC1), CD24, EpCAM, CA125, CA19-9, CA15-3, CEA, and Her2. The capture antibody against a microvesicle or exosomal surface biomarker may be a recombinant antibody or an antigen-binding fragment thereof. The capture antibody against a microvesicle or exosomal surface biomarker may be a monoclonal antibody, a polyclonal antibody or a humanized antibody.
The coating agent may be a capture antibody against a second GASP-1 fragment. The second GASP-1 fragment may be any fragment of GASP-1 (SEQ ID NO: 1), but different from the first GASP-1 fragment. For example, the second GASP-1 fragment may comprise an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). The second GASP-1 fragment may consist of an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). In one embodiment, the second GASP-1 fragment comprises the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2). In another embodiment, the second GASP-1 fragment consists of the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2). The capture antibody against a second GASP-1 fragment may be a recombinant antibody or an antigen-binding fragment thereof. The capture antibody against a second GASP-1 fragment may be a monoclonal antibody, a polyclonal antibody or a humanized antibody.
The anti-GASP-1 detection antibody may be biotinylated. The anti-GASP-1 detection antibody may bind specifically to GASP-1 (SEQ ID NO: 1) or a fragment thereof, for example, the peptide of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8), or a combination thereof. The anti-GASP-1 detection antibody may be a recombinant antibody or an antigen-binding fragment thereof. The antibody may be a monoclonal antibody, a polyclonal antibody or a humanized antibody.
The GASP-1 or its fragment in the sample may be in a cell, for example, in the cytoplasm or cell membrane of the cell, or outside of a cell. The GASP-1 or its fragment may be associated with a microvesicle or an exosome, for example, in or on the surface of a microvesicle or an exosome, or not associated with a microvesicle or an exosome. An exosome associated with GASP-1 or its fragment is a GASP-1 exosome. A GASP-1 exosome may be in a cell before it is released, for example, in the cytoplasm or in the cell membrane of the cell, or outside of a cell. A microvesicle associated with GASP-1 or its fragment is a GASP-1 microvesicle. A GASP-1 microvesicle may be on the cell membrane or outside the cell.
The sample may comprise about 0.001-1.000, 0.005-0.100, 0.005-0.05, 0.005-0.02, 0.01-0.015, 0.01-0.02, 0.01-0.05, 0.01-0.1, 0.01-0.2, 0.01-0.5, 0.01-1 ng/ml of the GASP-1 or a fragment thereof. The sample may comprise at least about 0.001, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5 or 10 ng/ml of the GASP-1 or its fragment. The sample may comprise no more than about 10, 5, 1, 0.5, 0.1, 0.05, 0.02, 0.01, 0.005, 0.002 or 0.001 ng/ml of the GASP-1 or its fragment.
The GASP-1 or its fragment in the sample may be from a biological fluid of a subject. The method may further comprise determining the concentration of the GASP-1 or a fragment thereof in the biological fluid in the subject. The biological fluid may be selected from the group consisting of plasma, serum, saliva, urine, and cerebrospinal fluid. The biological fluid may be serum.
The biological fluid may comprise about 0.001-1.000, 0.005-0.100, 0.005-0.05, 0.005-0.02, 0.01-0.015, 0.01-0.02, 0.01-0.05, 0.01-0.1, 0.01-0.2, 0.01-0.5, 0.01-1 ng/ml of the GASP-1 or a fragment thereof. The biological fluid may comprise at least about 0.001, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5 or 10 ng/ml of the GASP-1 or its fragment. The biological fluid may comprise no more than about 10, 5, 1, 0.5, 0.1, 0.05, 0.02, 0.01, 0.005, 0.002 or 0.001 ng/ml of the GASP-1 or its fragment.
The GASP-1 or its fragment in the sample may have been in the circulatory system of a subject. The term “circulatory system” used herein refers to the system that circulates blood and lymph through the body of the subject, consisting of the heart, blood vessels, blood, lymph, and the lymphatic vessels and glands. In the circulatory system, GASP-1 or its fragment may be associated with a microvesicle or an exosome, for example, in or on a microvesicle or an exosome. A microvesicle associated with a GASP-1 or its fragment is a GASP-1 microvesicle. A GASP-1 microvesicle may be on cell membrane. A GASP-1 microvesicle may be released and outside of a cell. An exosome associated with a GASP-1 or its fragment is a GASP-1 exosome. A GASP-1 exosome may be in a cell before it is released, for example, in the cytoplasm or in the cell membrane of the cell. A GASP-1 exosome may be outside of a cell. Alternatively, GASP-1 or its fragment in the circulatory system may not be associated with a microvesicle, an exosome or a cell, and is deemed to free.
The subject may be a mammal, preferably a human. The subject may be free of a cancer symptom. The subject may have cancer or may be predisposed to cancer. The cancer may be selected from the group consisting of breast cancer, bladder cancer, ovarian cancer, colon cancer, endometrial carcinoma, esophagus squamous cell carcinoma, glioma, head cancer, neck cancer, hepatocellular carcinoma, infiltrating ductal breast carcinoma, larynx cancer, lung cancer, melanoma, mucinous cystadenocarcinoma of ovary, pancreatic cancer, prostate cancer, renal cell carcinoma, small bowel malignant stromal tumor, and stomach adenocarcinoma. Preferably, the cancer is associated with elevated GASP-1 expression. For example, the cancer may be selected from the group consisting of breast cancer, prostate cancer, lung cancer, pancreatic cancer, liver cancer and glioblastoma.
The presence of GASP-1 or its fragment in a test biological fluid of a test subject may indicate that the test subject has cancer. The cancer may be associated with elevated GASP-1 expression (or overexpression of GASP-1). For example, a test biological fluid of a test subject having at least about 0.001, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 50, 100, 200, 500 or 1000 ng/ml of the GASP-1 or its fragment may indicate that the test subject has cancer. A test biological fluid of a test subject having a concentration of GASP-1 or its fragment at least about 5, 10, 50, 100, 500 or 1000 times higher than that in a control biological fluid in a symptom-free individual may indicate that the test subject has cancer. The control biological fluid and the test biological fluid are preferably from the same tissue type. The test subject may be the symptom-free individual, and the control biological fluid and the test biological fluid may be from the same tissue.
The presence of microvesicles or exosomes associated with GASP-1 or its fragment in a test biological fluid of a test subject may suggest that the test subject have cancer. The cancer may be associated with elevated GASP-1 expression (or overexpression of GASP-1). For example, a test biological fluid of a test subject having at least about 0.001, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 50, 100, 200, 500 or 1000 ng/ml of the GASP-1 or its fragment associated with the microvesicles or exosomes may indicate that the test subject has cancer. A test biological fluid of a test subject having a concentration of GASP-1 or its fragment associated with the microvesicles or exosomes at least about 5, 10, 50, 100, 500 or 1000 times higher than a control biological fluid of a symptom-free individual may indicate that the test subject has cancer. The control biological fluid and the test biological fluid are preferably from the same tissue type. The test subject may be a symptom-free individual, and the control biological fluid and the test biological fluid may be from the same tissue.
The method may further comprise comparing the concentration of the GASP-1 or a fragment thereof in the biological fluid in the subject with a concentration of the GASP-1 or a fragment thereof in a biological fluid in a symptom-free individual. The biological fluid of the symptom-free individual may have GASP-1 expression at a minimal level, for example, no more than about 10, 5, 1, 0.5, 0.1, 0.05, 0.02, 0.01, 0.005, 0.002 or 0.001 ng/ml of the GASP-1 or its fragment.
The subject may have received a cancer treatment. The biological fluid may be in the subject after the treatment. The cancer may be associated with elevated GASP-1 expression (or overexpression of GASP-1). The method may further comprise determining a concentration of the GASP-1 or its fragment in a biological fluid in the subject before the treatment. The method may further comprise comparing the concentration of the GASP-1 or its fragment in the biological fluid in the subject after the treatment with the concentration of the GASP-1 or a fragment thereof in the biological fluid in the subject before the treatment. A change in the concentration of the GASP-1 or its fragment in the biological fluid in the subject after the treatment may be used to assess the efficacy of the treatment. A reduction of the concentration of the GASP-1 or its fragment in the biological fluid in the subject after the treatment by, for example, at least about 5, 10, 50, 100, 500 or 1000 times may indicate that the treatment is effective.
If the subject is suspected of having prostate cancer, the method may further comprise comparing the concentration of the GASP-1 or a fragment thereof in the biological fluid in the subject with a concentration of the GASP-1 or a fragment thereof in a biological fluid in an individual having benign prostatic hyperplasia (BPH). A higher concentration of the GASP-1 or a fragment thereof in a biological fluid in a subject suspected of having prostate cancer than that in a biological fluid in an individual having benign prostatic hyperplasia (BPH) by, for example, at least about 5, 10, 50, 100, 500 or 1000 times, may indicate that the subject has prostate cancer. The subject may be tested positive or negative in a prostate specific antigen (PSA) test. In one embodiment, the subject is tested false positive in a PSA test. This method is useful for differentiating prostate cancer from BPH in a subject.
If the subject is suspected of having stage IV invasive breast cancer, the method may further comprise comparing the concentration of the GASP-1 or a fragment thereof in the biological fluid in the subject with a concentration of the GASP-1 or a fragment thereof in a biological fluid in an individual having inflammatory breast cancer. A concentration of the GASP-1 or a fragment thereof in a biological fluid in a subject suspected of having stage IV invasive breast cancer higher than that in a biological fluid in an individual having inflammatory breast cancer by, for example, at least about 5, 10, 50, 100, 500 or 1000 times, may indicate that the subject has stage IV invasive breast cancer. This method is useful for differentiating stable IV invasive breast cancer from inflammatory breast cancer in a subject.
The detection method of the present invention may further comprise detecting a cancer biomarker in the sample. The cancer biomarker is not GASP-1, and may be specific for breast cancer, bladder cancer, ovarian cancer, colon cancer, endometrial carcinoma, esophagus squamous cell carcinoma, glioma, head cancer, neck cancer, hepatocellular carcinoma, infiltrating ductal breast carcinoma, larynx cancer, lung cancer, melanoma, mucinous cystadenocarcinoma of ovary, pancreatic cancer, prostate cancer, renal cell carcinoma, small bowel malignant stromal tumor, and stomach adenocarcinoma. For example, the cancer may be selected from the group consisting of breast cancer, prostate cancer, lung cancer, pancreatic cancer, liver cancer or glioblastoma. Examples of the cancer biomarkers include Glypican-1 (GPC1), CD24, EpCAM, CA125, CA19-9, CA15-3, PSA, Her2, and CEA.
The detection method of the present invention is useful for diagnosis and/or assessment of cancer associated with elevated GASP-1 expression (or overexpression of GASP-1). This method may be used for cancer detection in a subject, especially early cancer in a symptom-free subject, based on the expression level of GASP-1 or its fragment in a biological fluid sample from the subject. This method may be used to assess cancer treatment or cancer progression in a subject based on the expression level of GASP-1 or its fragment in a biological fluid sample from the subject. Exemplary cancer includes breast cancer, bladder cancer, ovarian cancer, colon cancer, endometrial carcinoma, esophagus squamous cell carcinoma, glioma, head cancer, neck cancer, hepatocellular carcinoma, infiltrating ductal breast carcinoma, larynx cancer, lung cancer, melanoma, mucinous cystadenocarcinoma of ovary, pancreatic cancer, prostate cancer, renal cell carcinoma, small bowel malignant stromal tumor, and stomach adenocarcinoma. The detection method may provide high sensitivity of, for example, at least about 90%, 95%, 98%, 99% or 99.9%. In one embodiment, the sensitivity of the method is 100%. The detection method may provide high specificity of, for example, at least about 90%, 95%, 98%, 99% or 99.9%. In one embodiment, the specificity of the method is 100%.
The present invention also provides a kit for detecting G protein coupled receptor-associated sorting 1 (GASP-1) or a fragment thereof in a sample. The kit comprises a surface and an anti-GASP-1 detection antibody. A coating agent is immobilized to the surface and is selected from the group consisting of a first GASP-1 fragment, a conjugate of a protein, for example, bovine serum albumin (BSA) or casein, and the first GASP-1 fragment (protein-GASP-1 conjugate), a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, and a combination thereof. The first and second GASP-1 fragments are different. The kit may further comprise an instruction for carrying out the method of the present invention.
The present invention further provides a kit for detecting G protein coupled receptor-associated sorting 1 (GASP-1) or a fragment thereof in a sample. The kit comprises a surface, a coating agent and an anti-GASP-1 antibody. The coating agent is selected from the group consisting of a first GASP-1 fragment, a conjugate of a protein, for example, bovine serum albumin (BSA) or casein, and the first GASP-1 peptide (protein-GASP-1 conjugate), a capture antibody against a microvesicle or exosomal surface biomarker, a capture antibody against a second GASP-1 fragment, and a combination thereof. The first and second GASP-1 fragments are different. The kit may further comprise a first instruction for immobilizing the coating agent to the surface and a second instruction for carrying out the method of the present invention.
The coating agent in each kit of the present invention may be a first GASP-1 fragment. The first GASP-1 fragment may be any fragment of GASP-1 (SEQ ID NO: 1), preferably any GASP-1 peptide known to be exposed on the surface of a cell, a microvesicle or an exosome. For example, the first GASP-1 fragment may comprise an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). The first GASP-1 fragment may consist of an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). In one embodiment, the first GASP-1 fragment comprises the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2). In another embodiment, the first GASP-1 fragment consists of the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2).
The coating agent in each kit of the present invention may be a protein-GASP-1 conjugate of a protein and the first GASP-1 fragment. The protein may be BSA and the corresponding conjugate may be a BSA-GASP-1 conjugate. The protein may be casein, and the corresponding conjugate may be a casein-GASP-1 conjugate. The method may further comprise binding the anti-GASP-1 detection antibody specifically to the protein-GASP-1 conjugate, for example, the BSA-GASP-1 conjugate.
The coating agent in each kit of the present invention may be a capture antibody against a microvesicle or exosomal surface biomarker. The microvesicle or exosomal surface biomarker may be selected from the group consisting of CD9, CD63, CD81, and a combination thereof. The microvesicle or exosomal surface biomarker may be selected from other reported exosomal proteins such as Glypican-1 (GPC1), CD24, EpCAM, CA125, CA19-9, CA15-3, CEA, and Her2. The capture antibody against a microvesicle or exosomal surface biomarker may be a recombinant antibody or an antigen-binding fragment thereof. The capture antibody against a microvesicle or exosomal surface biomarker may be a monoclonal antibody, a polyclonal antibody or a humanized antibody.
The coating agent may be a capture antibody against a second GASP-1 fragment. The second GASP-1 fragment may be any fragment of GASP-1 (SEQ ID NO: 1), but different from the first GASP-1 fragment. For example, the second GASP-1 fragment may comprise an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). The second GASP-1 fragment may consist of an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), and GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8). In one embodiment, the second GASP-1 fragment comprises the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2). In another embodiment, the second GASP-1 fragment consists of the amino acid sequence of EEASPEAVAGVGFESK (SEQ ID NO: 2). The capture antibody against a second GASP-1 fragment may be a recombinant antibody or an antigen-binding fragment thereof. The capture antibody against a second GASP-1 fragment may be a monoclonal antibody, a polyclonal antibody or a humanized antibody.
The anti-GASP-1 detection antibody in each kit of the present invention may be biotinylated. The anti-GASP-1 detection antibody may bind specifically to GASP-1 (SEQ ID NO: 1) or a fragment thereof, for example, the peptide of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), CGSRTLADEDEAI (SEQ ID NO: 7), GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8), or a combination thereof. The anti-GASP-1 detection antibody may be a recombinant antibody or an antigen-binding fragment thereof. The anti-GASP-1 detection antibody may be a monoclonal antibody, a polyclonal antibody or a humanized antibody.
The term “about” as used herein when referring to a measurable value such as an amount, a percentage, and the like, is meant to encompass variations of ±20% or ±10%, more preferably ±5%, even more preferably ±1%, and still more preferably ±0.1% from the specified value, as such variations are appropriate.
The distribution of GASP-1 in pancreatic cells was visualized by immunohistochemical (IHC) staining with an anti-GASP-1 polyclonal antibody raised against a GASP-1 fragment consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2). The intensity of the staining indicates the GASP-1 expression level.
GASP-1 overexpression was surprisingly found in some pancreatic cells from a symptom-free individual. Sections of a pancreatic tissue from the symptom-free individual were stained with the anti-GASP-1 antibody. GASP-1 expression was found at a minimal level in some pancreatic cells (
The GASP-1 overexpression was observed in pancreatic ductal adenocarcinoma (PDAC), but not normal pancreatic cells. The cell samples were kindly supplied by Dr. Leland Chung of Cedars-Sinai Medical Center, Los Angeles Calif. The normal pancreatic cells showed GASP-1 expression at a minimal level (
The unexpected findings of the progressively high production of GASP-1 inside a symptom-free individual's pancreatic tissue cells and the accumulation of GASP-1 microvesicles on the surface of pancreatic cancer cells before they are released suggest a correlation between cancer initiation and GASP-1 overexpression, and GASP-1 may be an excellent early detection biomarker for pancreatic cancer.
Because the over-expressed GASP-1 by cancer cells appear to have been released from the cancer cells as microvesicles (GASP-1 microvesicles) or exosomes (GASP-1 exosomes), the GASP-1 in circulation (e.g., blood) may be either freed from the microvesicles or exosomes or remain on the surface of the microvesicles or exosomes. The continuing increase in the number of GASP-1 microvesicles or GASP-1 exosomes during cancer progression suggests that GASP-1 microvesicles or GASP-1 exosomes could also be used as a cancer biomarker to monitor cancer progression. Thus, an increase in GASP-1 microvesicles or GASP-1 exosome quantity in cancer cells and altered cargo expression may be considered as a potent biomarker for alteration of normal physiological states.
A highly sensitive GASP-1 competitive ELISA procedure was developed to detect and quantify GASP-1 or a fragment thereof in a sample. In particular, a conjugate of BSA (bovine serum albumin) and a GASP-1 peptide (BSA-GASP-1 conjugate) was used to coat a single plate to retain the GASP-1 peptide before detecting GASP-1 or a fragment thereof in a sample. The sequence of the GASP-1 peptide was EEASPEAVAGVGFESK (SEQ ID NO: 2).
An ELISA plate having multiple wells was coated with 100 μl of a solution containing the BSA-GASP-1 conjugate with shaking either overnight (e.g., 8-12 hours) at 4° C. or for 2 hours at room temperature. This was followed by the addition of 300 μl of 1% BSA solution and incubation for 20 min to block sites on the plate not covered by the BSA-GASP-1 conjugate. The solution was aspirated and washed once with Tris (tris-hydroxymethyl aminomethane)-buffed saline solution containing 0.05% Tween 20 (TBST). 50 μl of a diluted serum sample (or a standard GASP-1 peptide solution for standard curve construction) was added to wells of the plate. This was followed by the additional of 50 μl of 1:8000 biotinylated anti-GASP-1 antibody against the peptide of SEQ ID NO: 2 to the wells and incubation for 2 hours at room temperature with shaking. The final dilution in the well was 1:16,000 for the antibody and 1:8 for the sample in a volume of 100 μl. The plate was then aspirated and washed 3 times with 300 μl of TBS-Tween buffer (TBST). The TBST solution was then aspirated before 100 μl of diluted Streptavidin-HRP was added and incubated for 30 min to one hour at room temperature. The mixture was then aspirated and the plate was washed 3 times with 300 μl of TBST buffer. The TBST buffer was then aspirated before 100 μl of TMB (tetramethylbenzidine) solution was added and incubated with shaking for 10 to 30 min (stop the solution if the control well was highly blue) for color development. 50 μl of 1 N sulfuric acid was added to each well to stop the color development. The absorbance of wells was read at 450 nm and used to calculate the concentration of the GASP-1 or a fragment thereof. Ideally, the absorbance reading for a control well (without a competing peptide) should be around 1.2. Slightly higher or lower readings are acceptable. A representative standard curve is shown in
In the standard curve in
A sandwich ELISA was also developed to detect and quantify GASP-1 in a sample using two different antibodies, a capture antibody and a detection antibody that bind specifically to two different exposed peptides in GASP-1. Each GASP-1 peptide may comprise an amino acid sequence selected from the group consisting of EEASPEAVAGVGFESK (SEQ ID NO: 2), CSKSSPKAEEEEV (SEQ ID NO: 3), FWDGKEVSEEAGPC (SEQ ID NO: 4), EESNIDGTGEKAKL (SEQ ID NO: 5), WKEDEAISEATDR (SEQ ID NO: 6), and CGSRTLADEDEAI (SEQ ID NO: 7). One of the two antibodies may consist of SEQ ID NO: 2.
Separately, another sandwich ELISA was developed using an anti-GASP-1 capture antibody against GIVGSWFGAREETIRREAGSCSKSSPKAEE (SEQ ID NO: 8), corresponding to amino acids at 809-838 of GASP-1, and an anti-GASP-1 detection antibody against EEASPEAVAGVGFESK (SEQ ID NO: 2), corresponding to amino acids at 850-865 of GASP-1. The anti-GASP-1 detection antibody was biotinylated and the GASP-1 or its fragment captured by the anti-GASP-1 capture antibody was quantified by adding streptavin-HRP followed by detection with tetramethylbenzidine (TMB). The detection sensitivity can be substantially increased, for example, by attaching the detection antibody to polyHRP, which is a polymer containing many molecules of horseradish enzyme. The number of HRP in the polymer can be from 5 to 80 or higher and the HRP enzyme can be quantified colorimetrically with TMB, 3,3′-diaminobenzidine (DAB) or 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Alternatively, the HRP enzyme can be quantified by producing light when acting on chemiluminescent substrates (e.g., Enhanced Chemiluminescence by luminol).
The competitive GASP-1 ELISA as described in Example 2 was used to quantity GASP-1 levels in serum samples from 18 symptom-free (normal) individuals and 37 breast cancer patients. As shown in
In a 19-patient prostate cancer study, a clear separation in GASP-1 levels between normal individuals and cancer patients was found (
Unlike the PSA test which cannot differentiate between prostate cancer and benign prostatic hyperplasia (BPH), our GASP-1 ELISA shows that BPH patients do not overexpress GASP-1 (
Through collaboration with Lung Cancer Institute in Chengdu, China, a large population lung cancer clinical trial was conducted. Our results show that GASP-1 ELISA has an AUC value of 0.9395, which is better than all other reported lung cancer biomarkers (
Similarly, when we conducted the ELISA analysis using 40 pancreatic cancer patients and 18 normal individuals, only very minor overlaps were observed between cancer patients and symptom-free (normal) individuals with an AUC number of 0.9824 (
We also determined the GASP-1 levels between normal individuals and those with liver cancer and/or glioblastoma.
Because GASP-1 overexpression and their subsequently release as GASP-1 microvesicles or GASP-1 exosomes into circulation is cancer-specific, GASP-1 can be considered as a “universal” cancer biomarker. Such a universal cancer biomarker has been actively pursued by many but not discovered yet. As shown above, some of the cancer we tested such as breast, prostate and glioblastoma have an AUC number of 1.0 and one would also assume other cancers could also reach the perfect number. One reason for slightly lower AUC numbers in some of the cancers we tested could be due to deficiencies in current assessment of normal individuals based solely on the appearance of symptoms. According to this criterion, a normal individual could mean that symptoms have not shown up in that individual. Because it normally takes many years for cancer symptoms to show up and during this latent period, many biochemical reactions leading to cancer may have already started. The finding that overexpression of GASP-1 is one of the required steps for cancer initiation and GASP-1 is only overexpressed in cancer cells means that we can detect very early stage cancer well before symptoms show up by looking at the appearance and increase in serum GASP-1 levels. We have also found that some symptom-free (normal) individuals are having serum GASP-1 levels that are at our limit of detection of 0.01 ng/ml indicating that most likely they are the healthiest individuals. We also found that there is only a very small range of serum GASP-1 levels in all the normal individuals we analyzed, beyond that would indicate the presence of early cancer. It is interesting to note that some older “normal” individuals (those at 70, or 80, or 85 years old) are having serum GASP-1 levels approaching the upper range of GASP-1 levels normally would be indicative of early stage cancer. These individuals may already have very early stage cancer but their symptoms have not been detected yet. Therefore, slightly higher serum GASP-1 levels in normal individuals could indicate very early cancer stage even though they are symptom-free. A serum GASP-1 scale can be proposed to indicate cancer severity similar to serum cholesterol test.
As cancer progresses, more GASP-1 is overexpressed and released. The competitive GASP-1 ELISA assay of Example 2 may be used to monitor the effectiveness of cancer therapy. Because the levels of serum GASP-1 or its peptide fragments in symptom-free (normal) individuals were very low whereas those in cancer patients were very highly elevated, it is likely that an effective cancer treatment would be reduction of serum GASP-1 or its fragments levels toward that of normal individuals. In a pilot study shown in Table 1, a pancreatic cancer patient with a serum GASP-1 level of 4.7 ng/ml was treated with 2 cycles of gemcitabine plus abraxane (gem/abraxane). The serum GASP-1 level dropped 65% from 4.7 to 1.6 ng/ml. After two additional cycles of treatment, the serum GASP-1 level dropped further to 0.4 ng/ml, which is approaching the level for a normal individual. Therefore, levels of serum GASP-1 or its fragments could be used to monitor the effectiveness of cancer treatment.
Although the invention is illustrated and described herein with reference to specific embodiments, the invention is not intended to be limited to the details shown. Rather, various modifications may be made in the details within the scope and range of equivalents of the claims without departing from the invention.
This application is a continuation-in-part application of U.S. application Ser. No. 16/227,642, filed Dec. 20, 2018, which claims the benefit of U.S. Provisional Application No. 62/608,962, filed Dec. 21, 2017, the contents of each of which are incorporated herein by reference in their entireties for all purposes.
Number | Date | Country | |
---|---|---|---|
62608962 | Dec 2017 | US |
Number | Date | Country | |
---|---|---|---|
Parent | 16227642 | Dec 2018 | US |
Child | 16827145 | US |