Patent Application
20210190677
The present invention pertains to a sensor cover, and more particularly to a cover for a sensor used to measure properties of a solution into which air is mixed, and a sensor apparatus including same.
In various industrial production processes there is a need to measure properties of solutions such as the electrical conductivity or turbidity of the solution, or the concentration of specific components in the solution. Japanese Patent No. 3074781 (Patent Document 1) sets forth a method for manufacturing L-lysine. In this manufacturing method, a carbon source is added to maintain a carbon source concentration of 5 g/L or less in a culture solution. I.e., in this method, a culture solution is sampled at appropriate times and its carbon source concentration directly measured, or its pH or dissolved oxygen concentration is measured to detect deficiencies of the carbon source using changes therein as a way of measuring carbon source concentration to control feeding of the medium.
As noted in the invention set forth in the Patent Document 1, the properties of a solution can be measured relatively easily by removing a portion of the solution to be measured from the production process, but it is desirable from a manufacturing efficiency standpoint to measure in-line, without removing material from the manufacturing floor. However it can be difficult to measure solution properties, especially in-line, when gas bubbles mix into the solution to be measured. For example, bubbles of supplied oxygen or air, or bubbles of carbon dioxide gas occurring as the metabolic product of microorganisms in the culture, can become mixed into a culture solution while it is being aerobically cultured, thus adding noise to measurement values and increasing measurement errors.
Japanese Patent No. 4420168 (Patent Document 2) sets forth a turbidity sensor. This turbidity sensor has a stainless steel hollow semicircular tube member, on the bottom portion of which a test fluid intake opening and an automatically opening and closing swing valve are disposed, and on the top portion of which a foam draining hole is formed. In addition, the solution-contacting light measuring portion of the laser turbidimeter is disposed on the tip position thereof, on the interior of the hollow semicircular tube. For measurements using this turbidity sensor, the swing valve is first opened and test fluid in the hollow semicircular tube is exchanged. The swing valve is then closed, gas bubbles inside the hollow semicircular tube are discharged from the foam drain hole, and turbidity is measured after waiting for the turbidity targeted for detection to stabilize. In the invention of Patent Document 2, the effects of gas bubbles in the fluid under test are in this manner reduced.
Patent Document 1: Japanese Patent No. 3074781
Patent Document 2: Japanese Patent No. 4420168
However, in the turbidity sensor set forth in Patent Document 2 it is necessary to wait for detection values to stabilize after the swing valve is closed, which makes real time detection difficult. Also, the turbidity sensor of Patent Document 2 has the problem that it requires a swing valve or the like which can be opened and closed by remote operation, therefore the structure is complicated, and maintenance to ensure stable operation over a long time period is burdensome.
The present invention thus has the object of using a simple structure to provide a sensor cover capable of reducing the effects of gas bubbles mixed into a solution, and a sensor apparatus furnished with same.
In order to resolve the above-described problems, the present invention is a sensor cover for a sensor for measuring properties of a solution into which gas bubbles are mixed, comprising: a cover main body disposed to surround the sensor; wherein a lower transmitting portion for passing solution is provided on at least a portion of a lower surface of the cover main body, and an upper transmitting portion for passing solution is provided on at least a portion of an upper surface of the cover main body; and a large number of fine holes for passing solution are respectively formed on the lower transmitting portion and the upper transmitting portion, wherein the fine holes provided on the lower transmitting portion are formed to be smaller than the fine holes provided on the upper transmitting portion.
In the invention thus constituted, a lower transmitting portion and upper transmitting portion for passing solution are placed on a cover main body disposed to surround the sensor, therefore the solution to be measured can constantly flow in and out of the cover main body. As a result, solution within the cover main body is constantly exchanged, and the sensor disposed within the cover main body can continuously measure solution properties in real time. Also, the fine holes placed in the lower transmitting portion are formed to be smaller than the fine holes placed in the upper transmitting portion, therefore gas bubbles floating up from the bottom of the cover main body have difficulty passing through the fine holes on the lower transmitting portion, and gas bubbles can be effectively suppressed from penetrating into the cover main body. On the other hand, the fine holes in the upper transmitting portion are formed to be larger than the fine holes in the lower transmitting portion, therefore gas bubbles which have penetrated into the cover main body can easily float up within the cover main body to be discharged through the upper transmitting portion. As a result, accumulation in the cover main body of gas bubbles which have penetrated into the cover main body, and the resulting adverse influence on sensor measurement values, can be suppressed.
In the present invention the cover main body is preferably formed in an approximately cylindrical shape, and the sensor extends in an axial direction on an interior of the cover main body.
In the invention thus constituted, the cover main body is formed in an approximately cylindrical shape, so gas bubbles which have floated up from the lower side of the cover main body can easily flow up along the lower wall surface of the cover main body, and gas bubbles can be further suppressed from penetrating into the cover main body. Since the cover main body is formed in an approximately cylindrical shape, gas bubbles which penetrate the cover main body and float upward are collected in the highest part within the cover main body, and have difficulty contacting the sensor. Adverse influence on measurement can thus be minimized, even if gas bubbles penetrate into the cover main body.
In the present invention, preferably, the lower transmitting portion is placed on an entire surface of a lower semicircular portion of the approximately cylindrical cover main body, and the upper transmitting portion is placed on an entire surface of an upper semicircular portion of the cover main body.
In the invention thus constituted, the lower transmitting portion and upper transmitting portion are respectively placed over the entire surface of the lower semicircular portion and the upper semicircular portion, therefore the part of the cover main body through which solution passes can be made extremely large.
As a result, solution in the cover main body can be easily exchanged with outside solution, thus enabling accurate measurement of solution properties in real time.
In the present invention, preferably, the cover main body is formed of a thin metal plate, and the fine holes in the lower transmitting portion and the upper transmitting portion are approximately round holes made in the thin metal plate.
In the invention thus constituted, fine holes in the lower transmitting portion and upper transmitting portion are formed by holes made in a metal thin plate, so compared to the case in which the lower transmitting portion or upper transmitting portion are formed of a mesh product formed by weaving strands of wire, solution has difficulty adhering to the lower transmitting portion or upper transmitting portion, and maintainability of the sensor cover is improved. Forming fine holes in the lower transmitting portion and upper transmitting portion as holes disposed in thin metal plate results in lower propensity to breakage than with mesh, enabling the formation of a high-durability cover main body.
In the present invention, preferably, a diameter of the fine holes provided in the upper transmitting portion is between 1.5 and 5 times a diameter of the fine holes provided in the lower transmitting portion.
In the invention thus constituted, the diameter of fine holes disposed in the upper transmitting portion is set to between 1.5 and 5 times the diameter of fine holes disposed in the lower transmitting portion, thus suppression of penetration by gas bubbles into the cover main body can be appropriately balanced against the discharge of those gas bubbles which do penetrate into the cover main body, so the influence of gas bubbles on the sensor can be effectively suppressed.
In the present invention, preferably, the diameter of fine holes in the lower transmitting portion is 100 μm to 400 μm, and more preferably 150 μm to 400 μm.
In the invention thus constituted, fine holes placed on the lower transmitting portion are formed at a diameter of 100 μm to 400 μm, therefore the penetration of gas bubbles of a size that could easily adversely influence sensor measurement values can be effectively suppressed, while solution is still permitted to flow into the cover main body.
In the present invention, preferably, the cover main body is placed to project diagonally downward from a side wall surface of a container in which the solution is held, and a measurement portion of the sensor is disposed close to an end of the cover main body.
In the invention thus constituted, the cover main body projects diagonally downward from the side wall surface of the container in which the solution is held, therefore gas bubbles floating up from below and reaching the cover main body can easily flow upward along the bottom surface of the cover main body, and penetration into the cover main body can be effectively suppressed. Gas bubbles that have penetrated into the cover main body collect in the base end portion of the cover main body located above, traveling away from the measurement portion of the sensor disposed close to the end of the cover main body, reducing adverse influence on measurement.
The sensor apparatus of the present invention comprises a sensor sensor for measuring properties of a solution; and the cover of any one of Claims 1 through 7, disposed to surround the sensor.
In addition, the solution property measurement method of the present invention comprises a step for preparing the sensor apparatus of the present invention; and a step for acquiring a measurement signal from the sensor apparatus inserted into the solution.
The method of the present invention for manufacturing metabolic products in an aerobic culture comprises a step for preparing the sensor apparatus of the present invention a step for acquiring a measurement signal from the sensor apparatus inserted into a culture solution; a step for aerobic culturing in the culture solution; and a step for tracking changes in concentration of the metabolic products in the aerobic culture and/or changes in concentration of a culture raw material based on the measurement signal.
Using the sensor cover and sensor apparatus comprising same of the present invention, the influence of gas bubbles blended in a solution can be reduced using a simple structure.
Next, referring to the attached figures, we explain preferred embodiments of the invention.
As shown in
Next, referring to
As shown in
The sensor cover 10 has an approximately cylindrical cover main body 14, and a flange portion 16 placed at the base end of this cover main body 14 and affixed to the side wall surface 2a of fermenter 2.
The cover main body 14 is formed of thin stainless steel plate, constituted as an approximately cylindrical shape, closed at one end, and disposed to surround sensor 12. As described below, many fine holes are disposed over the entire surface of cover main body 14 so that solution inside fermenter 2 can flow into cover main body 14 through these fine holes. Note that the cover main body can be constituted in non-cylindrical shapes such as a parallelepiped. The cover main body can also be formed of metal other than stainless steel, or of resins such as polytetrafluoroethylene, and is preferable formed of a material resistant to breakage by the flow of solution resulting from mixing.
The flange portion 16 is a stainless steel disk; cover main body 14 is affixed to the center thereof by welding to form an integral piece. Also, cover main body 14 is perpendicularly attached to the flat surface of flange portion 16. A bolt hole 16a is formed in flange portion 16, and flange portion 16 is affixed to the outside of side wall surface 2a by an affixing bolt 18a. A packing 18b is disposed between flange portion 16 and side wall surface 2a to secure a watertight seal between flange portion 16 and side wall surface 2a. In addition, as shown in
In the present embodiment a transmissive/reflective near-infrared spectral sensor (NIR sensor) is used for sensor 12; amino acid concentration in culture solution L is measured by near-infrared analysis. However the sensor cover 10 of the present embodiment can be applied to various types of sensors in addition to NIR sensors, such as spectral sensors using ultraviolet or visible light, other optical sensors, or electromagnetic sensors for measuring permittivity or conductance, and these sensors can be combined to constitute a sensor apparatus.
As shown in
Next, referring to
As described above, a large number of fine holes are formed on the entire surface of the cover main body 14 portion of a sensor cover 10 according to an embodiment of the present invention, such that surrounding solution is able to flow into the interior of cover main body 14. In the sensor cover 10 of the present embodiment, the size of each fine hole formed on the lower surface of cover main body 14 and the size of each fine hole formed in the upper surface thereof are different. I.e., a lower transmitting portion 14a is provided for passing solution to the lower surface of cover main body 14, and an upper transmitting portion 14b is provided for passing solution to the upper surface thereof, and fine holes formed on the lower transmitting portion 14a are formed to be smaller than the fine holes formed in the upper transmitting portion 14b.
As shown in
Note that in this Specification, the “lower surface” of cover main body 14 means the surface on which light impinges when light is illuminated from vertically below onto sensor cover 10, shown installed in a state of use, and “upper surface” means the surface on which light falls when illuminated from vertically above. In the present embodiment a large number of fine holes are formed in the entirety of the “lower surface” and “upper surface” of cover main body 14, but it is not necessarily required that fine holes be formed on the entire surface; they may be formed in a portion only. In addition, in the present embodiment fine holes are also provided on the end surface 14c of cover main body 14, but it is also acceptable not to provide fine holes on the end surface 14c.
Next, as shown in
Here, a lower transmitting portion 14a, in which round holes with a diameter D=approximately 180 μm are arrayed at a pitch P=approximately 320 μm, has a porosity of 28.7%, which roughly corresponds to an 80# mesh (80 mesh: a mesh in which 80 lines per inch are arrayed horizontally and vertically). In addition, upper transmitting portion 14b, in which round holes with a diameter D=approximately 850 μm are arrayed at a pitch P=approximately 1270 μm has a porosity of 40.6%, which roughly corresponds to a 20# mesh. An upper transmitting portion 14b, in which round holes with a diameter D=approximately 350 μm are arrayed at a pitch P=approximately 630 μm, has a porosity of 28%, which roughly corresponds to a 40# mesh.
Note that when round holes with a diameter D=approximately 100 μm are arrayed at a pitch P=approximately 210 μm, porosity is approximately 20.5%, which corresponds to a 150# mesh, and when round holes with a diameter D=approximately 150 μm are arrayed at a pitch P=approximately 250 μm, porosity is approximately 32.5%, roughly corresponding to a 100# mesh.
Note that in the present embodiment, lower transmitting portion 14a and upper transmitting portion 14b are constituted by forming a large number of fine holes in a thin plate, but lower transmitting portion 14a or upper transmitting portion 14b may also be constituted by weaving or knitting to combine fine strands into a mesh. In this case the “fine holes” would be formed as the spaces between the strands constituting the mesh, and the “fine hole diameter” would mean the distance between adjacent strands. The diameter of fine holes provided in lower transmitting portion 14a is preferably set between approximately 100 μm and 400 μm. The diameter of fine holes provided in lower transmitting portion 14a is more preferably set between approximately 150 μm and 400 μm. To facilitate discharge of gas bubbles which enter into the cover main body, fine holes disposed on the upper transmitting portion 14b are preferably formed to be larger than the diameter of the fine holes on the lower transmitting portion 14a. However, it is desirable to set the diameter of the fine holes in upper transmitting portion 14b so that gas bubbles can be sufficiently suppressed from flowing in through upper transmitting portion 14b as the result of downward solution flow. The diameter of fine holes placed on upper transmitting portion 14b is preferably set to between approximately 1.5 times and approximately 5 times the diameter of the fine holes on lower transmitting portion 14a. This allows an appropriate balance between the suppression of gas bubble penetration into cover main body 14 and the discharge of those gas bubbles which do penetrate into cover main body 14.
Next, referring to
First, a method has been known for some time whereby the effect of gas bubbles is reduced by covering a sensor with a mesh-shaped strainer when measuring properties of a solution into which gas bubbles are mixed. However with conventional covers of this type, as shown in
On the other hand, as shown in
By contrast, as shown in
On the other hand, if gas bubbles approach from below, small bubbles among them penetrate into cover main body 14 through lower transmitting portion 14a. However, large diameter fine holes are formed on upper transmitting portion 14b, therefore small gas bubbles which penetrate through lower transmitting portion 14a float up within cover main body 14 and are easily discharged to the outside of cover main body 14 through upper transmitting portion 14b. Therefore the accumulation of penetrating gas bubbles inside cover main body 14 over a long time period, and resultant growth into large gas bubbles, can be stopped.
Gas bubbles which approach sensor cover 10 do not all approach from below, but because buoyancy from the solution is always acting on the gas bubbles, the fraction of gas bubbles approaching sensor cover 10 from below is high, and the penetration of these gas bubbles is greatly reduced by the lower transmitting portion 14a. In addition, penetration by some of the gas bubbles which approach sensor cover 10 from the side, etc. is also stopped by upper transmitting portion 14b.
Because cover main body 14 is disposed to project diagonally downward, gas bubbles penetrating into cover main body 14 move upward to the base end portion of cover main body 14. On the other hand, the measurement portion 12b of the sensor probe 12a disposed inside cover main body 14 is disposed close to the end portion of cover main body 14, such that gas bubbles inside cover main body 14 are moved so as to travel away from the sensor 12 measurement portion 12b. Thus the influence on measurement by gas bubbles which have penetrated into cover main body 14 can be further reduced.
By the above actions, the influence of gas bubbles on measurement is effectively reduced using the sensor cover 10 of the present embodiment.
Next, referring to
First, as described above, in the present embodiment sensor 12 is an NIR sensor, and light received at sensor probe 12a is guided to measurement instrument main unit 4 (
Next, referring to
Here an aerobic culture is cultured in the fermenter 2 used in the amino acid concentration estimation examples of
In the example shown in
Using a sensor cover 10 according to an embodiment of the present invention, a lower transmitting portion 14a and upper transmitting portion 14b for passing solution are formed on a cover main body 14 disposed to a surround sensor 12 (
Using a sensor cover 10 according to the present embodiment, cover main body 14 is formed in an essentially cylindrical shape (
Also, in the sensor cover 10 of the embodiment, the lower transmitting portion 14a and upper transmitting portion 14b are respectively provided on the entire surface of the lower semicircular portion and upper semicircular portion thereof (
Also, with the sensor cover 10 of the present embodiment, the fine holes in lower transmitting portion 14a and upper transmitting portion 14b are formed by holes made in thin metal plate (
In addition, with the sensor cover 10 of the present embodiment, the cover main body 14 projects diagonally downward from the side wall surface 2a of fermenter 2, which is a solution-holding container (
Above we have explained preferred embodiments of the present invention, but various changes may be added to the above-described embodiments. In particular, in the above-described embodiments of the invention the concentration of amino acid in a culture solution in which gas bubbles are mixed is estimated by spectral analysis, but the present invention may be applied to measure the properties of any desired solution in which gas bubbles are mixed. Also, in the above-described embodiment the invention was applied to measure the concentration of particular components in a solution, but the invention may also be applied to a sensor cover for any desired sensor used to measure properties of a solution itself, such as dielectric constant, conductivity, and the like.
In the above-described embodiment of the invention, the invention was applied to the measurement of amino acid concentration in a culture solution, but an aerobic culture can be accurately controlled by applying the invention to the measurement of various component concentrations not limited to amino acid concentration, thereby increasing the accumulation and yield of products in the culture solution. For example, by applying the invention to the measurement of carbon source concentration as set forth in Patent Document 1 described above, carbon source concentration can be measured in-line.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2018-128836 | Jul 2018 | JP | national |
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/JP2019/025828 | Jun 2019 | US |
| Child | 17136132 | US |
