Claims
- 1. A method for detecting an analyte, said method comprising:
providing a sensor comprising a substrate coated with a free electron metal, a matrix layer disposed on said free electron metal, said matrix layer comprising an organic compound, wherein said organic compound has a boronic acid complexing moiety and, a boronic acid moiety complexed to said boronic acid complexing moiety; contacting said sensor with said analyte to elicit a response; and measuring said response, thereby detecting said analyte.
- 2. The method for detecting an analyte of claim 1, wherein said matrix is a self-assembled monolayer.
- 3. The method for detecting an analyte of claim 1, wherein said matrix is a mixed self-assembled monolayer.
- 4. The method for detecting an analyte of claim 1, wherein said free electron metal is a member selected from the group consisting of copper, silver, aluminum, platinum and gold.
- 5. The method for detecting an analyte of claim 4, wherein said free electron metal is gold.
- 6. The method for detecting an analyte of claim 1, wherein said organic compound having said boronic acid complexing moiety is of the formula
- 7. The method for detecting an analyte of claim 6, wherein said organic compound having said boronic acid complexing moiety is of the formula:
- 8. The method for detecting an analyte of claim 7, wherein R′ is an optionally substituted alkylene group, which is optionally interrupted by a heteroatom and which is at least about 8 carbon atoms in length.
- 9. The method for detecting an analyte of claim 7, wherein R′ is an optionally substituted alkylene group, which is optionally interrupted by a heteroatom and which is between about 8 carbon atoms to about 40 carbon atoms in length.
- 10. The method for detecting an analyte of claim 7, wherein m is 1.
- 11. The method for detecting an analyte of claim 7, wherein Y has the formula:
- 12. The method for detecting an analyte of claim 11, wherein said organic compound having said boronic acid complexing moiety is of the formula
- 13. The method for detecting an analyte of claim 12, wherein R′ is an optionally substituted alkylene group, which is optionally interrupted by a heteroatom and which is at least about 8 carbon atoms in length.
- 14. The method for detecting an analyte of claim 12, wherein R′ is an optionally substituted alkylene group, which is optionally interrupted by a heteroatom and which is between about 8 carbon atoms to about 40 carbon atoms in length.
- 15. The method for detecting an analyte of claim 1, wherein said matrix comprises an organic compound of the formula:
- 16. The method for detecting an analyte of claim 15, wherein said nonspecific binding inhibitor is a member selected from the group consisting of an oligo(ethylene glycol), a poly(ethylene glycol), a branched OEG, a branched PEG, an oligo(peptide), an oligo(propylene sulfoxide), a sugar, a sugar alcohol, and a dendrimer.
- 17. The method for detecting an analyte of claim 15, wherein Z is a member selected from the group consisting of H, CH3, OH, OCH3, CO2CH3, C(O)NH2, C(O)NHCH3, C(O)N(CH3)2 or SO2NH2.
- 18. The method for detecting an analyte of claim 15, wherein said matrix layer comprises a combination of polymers having the formula:
- 19. The method for detecting an analyte of claim 18, wherein the mole percent of Polymer A is less than about 10% of the total mole percent of Polymer A and Polymer B.
- 20. The method for detecting an analyte of claim 18, wherein the mole percent of the Polymer A is less than about 5% of the total mole percent of Polymer A and Polymer B.
- 21. The method for detecting an analyte of claim 15, wherein R′ is an optionally substituted alkylene group, which is optionally interrupted by a heteroatom and which is at least about 8 carbon atoms in length.
- 22. The method for detecting an analyte of claim 15, wherein R′ is an optionally substituted alkylene group, which is optionally interrupted by a heteroatom and which is between about 8 carbon atoms to about 40 carbon atoms in length.
- 23. The method for detecting an analyte of claim 1, further comprising a boronic acid moiety complexed to said boronic acid complexing moiety.
- 24. The method for detecting an analyte of claim 21, wherein said boronic acid moiety comprises a bioactive species.
- 25. The method for detecting an analyte of claim 22, wherein said boronic acid moiety is of the formula:
- 26. The method for detecting an analyte of claim 25, wherein said BAS is a member selected from the group consisting of a protein, a polypeptide, a polypeptide fragment, a nucleic acid, a carbohydrate, a receptor, a hormone, a toxin, a vesicle, a liposome and a cell.
- 27. The method for detecting an analyte of claim 26, wherein said BAS is a protein.
- 28. The method for detecting an analyte of claim 27, wherein said protein is selected from the group consisting of a capture protein, an antigen, an antibody, protein A, protein G, protein A/G, an enzyme, a lectin, and avidin.
- 29. The method for detecting an analyte of claim 25, wherein said boronic acid moiety has the formula:
- 30. The method for detecting an analyte of claim 25, wherein said boronic acid moiety has the formula:
- 31. The method for detecting an analyte of claim 25, wherein said boronic acid moiety has the formula:
- 32. The method for detecting an analyte of claim 1, wherein said sensor surface is an array of sensor surfaces.
- 33. The method for detecting an analyte of claim 1, wherein said sensor surface is used in a surface plasmon resonance sensing or in a potentiometric sensing.
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Patent Application Nos. 60/281,085, 60/281,093, and 60/281,094, all filed on Apr. 2, 2001. This application further claims the benefit of U.S. Provisional Application No. 60/360,798, filed Mar. 1, 2002. Each of the foregoing applications are hereby incorporated by reference in their entirety for all purposes. This application incorporates by reference, U.S. application Ser. No. ______, filed on even date herewith, bearing Attorney Docket No. 017635-001610US, and entitled “SENSOR SURFACES FOR DETECTING ANALYTES”.
Provisional Applications (4)
|
Number |
Date |
Country |
|
60281085 |
Apr 2001 |
US |
|
60281093 |
Apr 2001 |
US |
|
60281094 |
Apr 2001 |
US |
|
60360798 |
Mar 2002 |
US |