Claims
- 1. A process for separation of the non-polar components of vegetable oil, the process comprising:
(a) preparing a vegetable oil sample for separation; (b) introducing an aliquot of known mass of the vegetable oil sample into a pre-packed silica separation column of at least about 5 grams; and (c) eluting the non-polar components of the aliquot with a solution comprising petroleum ether and diethyl ether having a petroleum ether:diethyl ether ratio from about 92:8 to about 82:18.
- 2. The process of claim 1, wherein said pre-packed silica separation column is a 5 gram column.
- 3. The process of claim 1, wherein said pre-packed silica separation column is a 10 gram column.
- 4. The process of claim 1, wherein said petroleum ether:diethyl ether ratio is from about 91:9 to about 83:17.
- 5. The process of claim 4, wherein said petroleum ether:diethyl ether ratio is from about 90:10 to about 84:16.
- 6. The process of claim 5, wherein said petroleum ether:diethyl ether ratio is from about 89:11 to about 85:15.
- 7. The process of claim 6, wherein said petroleum ether:diethyl ether ratio is from about 88:12 to about 86:14.
- 8. The process of claim 7, wherein said petroleum ether:diethyl ether ratio is about 87:13.
- 9. The process of claim 1, further comprising rinsing residual vegetable oil and/or crystallized fat residue after elution with petroleum ether, thereby separating residual non-polar components.
- 10. The process of claim 9, wherein said crystallized fat is melted prior to said rinsing.
- 11. A process for calculating the amount of polar components in a vegetable oil, the process comprising:
(a) preparing a vegetable oil sample for separation; (b) introducing an aliquot of known mass of the vegetable oil sample into a pre-packed silica separation column of at least about 5 grams; (c) eluting the non-polar components of the aliquot with a solution comprising petroleum ether and diethyl ether having a petroleum ether:diethyl ether ratio of from about 92:8 to about 82:18; (d) drying the eluted non-polar components to remove the solvent, thereby obtaining a mass of dried non-polar components; and (e) calculating the amount of polar components in the aliquot.
- 12. The process of claim 11, wherein said pre-packed silica separation column is a 5 gram column.
- 13. The process of claim 11, wherein said pre-packed silica separation column is a 10 gram column.
- 14. The process of claim 11, wherein said petroleum ether:diethyl ether ratio is from about 91:9 to about 83:17.
- 15. The process of claim 14, wherein said petroleum ether:diethyl ether ratio is from about 90:10 to about 84:16.
- 16. The process of claim 15, wherein said petroleum ether:diethyl ether ratio is from about 89:11 to about 85:15.
- 17. The process of claim 16, wherein said petroleum ether:diethyl ether ratio is from about 88:12 to about 86:14.
- 18. The process of claim 17, wherein said petroleum ether:diethyl ether ratio is about 87:13.
- 19. The process of claim 11, wherein step (c) further comprises rinsing residual vegetable oil and/or crystallized fat residue after elution with petroleum ether, thereby separating residual non-polar components.
- 20. The process of claim 19, wherein said crystallized fat is melted prior to said rinsing.
- 21. The process of claim 11, wherein at step (e) said calculating comprises subtracting the mass of the dried non-polar components from the mass of the aliquot of vegetable oil sample.
- 22. The process of claim 11 in which steps (c), (d), and (e) comprise the following:
(c) eluting the polar components of the aliquot with diethyl ether; (d) drying the eluted polar components to remove the diethyl ether; and (e) calculating the amount of non-polar components in the aliquot.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60/397,107, filed Jul. 22, 2002, the entire contents of which are hereby incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60397107 |
Jul 2002 |
US |