SGER: BAC end Sequencing to Quality Control BAC Libraries

Information

• NSF Award
• 0306190

Owner

• Institute for Genomic Research

• Award Id

  0306190

• Award Effective Date

  1/15/2003 - 21 years ago

• Award Expiration Date

  12/31/2005 - 18 years ago

• Award Amount

  $ 73,812.00

• Award Instrument

  Standard Grant

0306190<br/>Zhao<br/><br/>Due to their large insert size and stability, Bacterial Artificial Chromosome (BAC) clones have emerged as an essential component in genome mapping, DNA sequencing, as well as comparative and functional genomics projects to answer evolutionary, agricultural and biomedical questions. As such, the National Science Foundation (NSF) has initiated a program that funds the construction of 31 animal BAC libraries and 32 plant BAC libraries from species spanning the phylogenetic tree of life. Clearly, these libraries should be of high quality to be most useful. Quality control therefore becomes an essential requirement. BAC end sequencing provides several pieces of critical information about a BAC library regarding contaminations, randomness and insert size. Here we propose to end sequence approximately 100 random clones from each library and conduct sequence analyses to assess the quality of the 63 BAC libraries that are being constructed under the NSF program.

• Program Officer

  Steven L. Klein

• Min Amd Letter Date

  1/10/2003 - 21 years ago

• Max Amd Letter Date

  8/30/2005 - 19 years ago

• ARRA Amount

• Name

  Institute for Genomic Research

• City

  Rockville

• State

  MD

• Country

  United States

• Address

  9712 Medical Center Drive

• Postal Code

  208500334

• Phone Number

  3017957591

• First Name

  Shaying

• Last Name

  Zhao

• Email Address

  szhao@bmb.uga.edu

• Start Date

  1/10/2003 12:00:00 AM

• End Date

  08/30/2005

• First Name

  Norman

• Last Name

  Lee

• Email Address

  nhlee@tigr.org

• Start Date

  8/30/2005 12:00:00 AM

• Name

  Other Applications NEC

• Code

  99