Shared Resources Core 2: Quantitative Proteomics Core

Information

  • Research Project
  • 10269910
  • ApplicationId
    10269910
  • Core Project Number
    P01CA196539
  • Full Project Number
    5P01CA196539-07
  • Serial Number
    196539
  • FOA Number
    PAR-18-290
  • Sub Project Id
    6480
  • Project Start Date
    9/9/2015 - 8 years ago
  • Project End Date
    8/31/2025 - a year from now
  • Program Officer Name
  • Budget Start Date
    9/1/2021 - 2 years ago
  • Budget End Date
    8/31/2022 - a year ago
  • Fiscal Year
    2021
  • Support Year
    07
  • Suffix
  • Award Notice Date
    9/15/2021 - 2 years ago
Organizations

Shared Resources Core 2: Quantitative Proteomics Core

Project Summary The Quantitative Proteomics core led by Dr. Benjamin Garcia will provide cutting-edge mass spectrometry (MS) based proteomics technologies to the P01 Project team members to help elucidate biochemical mechanisms involved in histone and histone-like mutations in human cancers. These MS approaches will include quantitative experiments to understand protein expression and post-translational modifications (PTMs) on histone and non-histone proteins from both cultured and primary cell lines, tissue/tumors and formalin fixed paraffin embedded (FFPE) archived samples. Although most researchers in the epigenetics and chromatin biology fields utilize antibody based methods for histone modification characterization, antibodies have many technical issues (such as epitope occlusion), which confound the analyses. Mass spectrometry therefore provides a more unbiased and complementary approach, that is also more sensitive and accurate for protein PTM analysis. The Garcia Lab has for over a decade now developed methods for histone PTM analyses. Here in this propose we plan to continue to expand our proteomics toolbox to allow P01 team members to be able to characterize 500 histone PTM sites in rapid fashion, perform quantitative analyses of histone combinatorial modifications, and determine which oncogenic histone mutations affect chromatin structure. Additionally, we will explore the downstream protein signaling pathways that are altered in epigenetically driven human cancers, as transcriptional profiles do not always provide the most accurate pictures of protein expression. !

IC Name
NATIONAL CANCER INSTITUTE
  • Activity
    P01
  • Administering IC
    CA
  • Application Type
    5
  • Direct Cost Amount
    260915
  • Indirect Cost Amount
    0
  • Total Cost
  • Sub Project Total Cost
    220177
  • ARRA Funded
    False
  • CFDA Code
  • Ed Inst. Type
  • Funding ICs
    NCI:220177\
  • Funding Mechanism
    Non-SBIR/STTR RPGs
  • Study Section
    ZCA1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    ROCKEFELLER UNIVERSITY
  • Organization Department
  • Organization DUNS
    071037113
  • Organization City
    NEW YORK
  • Organization State
    NY
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    100656399
  • Organization District
    UNITED STATES