Claims
- 1. An isolated nucleotide sequence, comprising a promoter sequence operably associated with a heterologous nucleotide sequence, wherein said promoter sequence comprises a fragment of SEQ ID NO: 3 and specifically drives expression of said heterologous nucleotide sequence in shoot meristems.
- 2. The isolated nucleotide sequence of claim 1, further comprising a transcriptional and translational initiation region.
- 3. The isolated nucleotide sequence of claim 1, further comprising a transcriptional termination region functional in a plant cell.
- 4. The isolated nucleotide sequence of claim 1, wherein said heterologous nucleotide sequence encodes a Bacillus thuringiensis toxin.
- 5. The isolated nucleotide sequence of claim 1, wherein said heterologous nucleotide sequence encodes the LEAFY gene product.
- 6. A transgenic plant cell comprising a promoter sequence operably associated with a heterologous nucleotide sequence, wherein said promoter sequence comprises a fragment of SEQ ID NO: 3 and specifically drives expression of said heterologous nucleotide sequence in a shoot meristem cell.
- 7. The transgenic plant cell of claim 6, wherein said transgenic plant cell is a tobacco plant cell.
- 8. The transgenic plant cell of claim 6, wherein said transgenic plant cell is an Arabidopsis plant cell.
- 9. The transgenic plant cell of claim 6, further comprising a selectable marker.
- 10. A transgenic plant comprising a promoter sequence operably associated with a heterologous nucleotide sequence, wherein said promoter sequence comprises a fragment of SEQ ID NO: 3 and specifically drives expression of said heterologous nucleotide sequence in shoot meristem tissue.
- 11. The transgenic plant of claim 10, wherein said transgenic plant is a tobacco plant.
- 12. The transgenic plant of claim 10, wherein said transgenic plant is an Arabidopsis thaliana plant.
- 13. The transgenic plant of claim 10, further comprising a selectable marker.
- 14. An expression vector comprising a promoter sequence operably associated with a heterologous nucleotide sequence, wherein said promoter sequence comprises a fragment of SEQ ID NO: 3 and specifically drives expression of said heterologous nucleotide sequence in shoot meristems.
- 15. The expression vector of claim 14, wherein said expression vector is selected from the group consisting of: a retroviral vector, a Ti plasmid and Cauliflower mosaic virus (CaMV).
- 16. The expression vector of claim 14, wherein said expression vector comprises a nucleotide sequence encoding a selectable marker.
- 17. A method of providing increased transcription of a nucleic acid sequence in shoot meristem tissue of a plant, said method comprising:transforming a plant with a nucleic acid construct comprising a promoter sequence operably associated with a heterologous nucleotide sequence, wherein said promoter sequence comprises a fragment of SEQ ID NO: 3 and specifically drives expression of said heterologous nucleotide sequence in shoot meristems detecting increased expression of said heterologous nucleotide sequence.
- 18. The method of claim 17, wherein said transforming a plant comprises transforming an Arabidobsis thaliana plant.
- 19. The method of claim 17, wherein said transforming a plant comprises transforming a tobacco plant.
- 20. The method of claim 17, wherein said transforming a plant comprises transforming said plant with Agrobacterium tumefaciens.
- 21. The method of claim 17, wherein said heterologous nucleic acid sequence encodes a Bacillus thuringiensis toxin.
Parent Case Info
This is a continuation of U.S. application Ser. No. 08/693,457, filed Aug. 7, 1996, now U.S. Pat. No. 5,880,330.
STATEMENT AS TO FEDERALLY SPONSORED RESEARCH
This invention was made with Government support under Grant No. IBN-9406948 awarded by the National Science Foundation. The Government has certain rights in this invention.
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Continuations (1)
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Number |
Date |
Country |
Parent |
08/693457 |
Aug 1996 |
US |
Child |
09/265731 |
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US |