The disclosure relates to analysis of a skinprint, such as a fingerprint. The analysis may confirm the presence of a skinprint and may also provide an indication of the quality and/or quantity of the skinprint. The analysis may also confirm the identity of the skinprint.
Skinprints comprise eccrine sweat and may contain other constituents that may form a target for a diagnostic test. The applicant has developed a range of techniques for detecting the presence of one or more analytes in skinprints.
Characteristics of skinprints may vary substantially, for example in terms of area of skinprint and quantity of print substances present in the skinprint. These and other characteristics may contribute to a measure of skinprint quality/quantity. Skinprint quality/quantity may considerably affect the ease or otherwise of analysing the skinprint both for identification purposes and also for the purpose of detecting one or more analytes in the skinprint, especially when seeking quantitative analysis of one or more analytes.
By way of example, a good quality skinprint, on which the detection of one or more analytes may be most straightforward, may be one that is provided by a user depositing a firm impression of unwashed skin on a surface. By contrast, a reduced quality skinprint may be provided by a user who has recently washed the relevant area of skin and/or who provides only a minimal force when depositing the impression on a surface.
In some circumstances, for example, it may be that a user deliberately washes their hands in anticipation of being asked to provide a fingerprint and also uses only a minimal force when leaving the fingerprint.
It may be beneficial to analyse a skinprint for a measure of quality/quantity for a number of reasons. Such reasons may include (but are not necessarily limited to): confirming that a skinprint is present at all; confirming (where a skinprint is present) that the skinprint is of sufficient quality/quantity to facilitate a meaningful analysis of analytes; determining (where a skinprint is present) whether quality/quantity is sufficient for a quantitative analysis of analytes to be performed.
In addition, providing a straightforward quality check may avoid cost and inefficiency associated with analyte testing on a sample that is sub-standard or even absent altogether.
A quality check may be undertaken before or after collecting a skinprint for diagnostic analysis as well as being an integral part of a diagnostic analysis of materials such as metabolites in the skinprint.
It is known to use a quartz crystal microbalance to measure small mass increments. This technique does not lend itself well to robust in-the-field determination of fingerprint or skinprint mass measurement. Furthermore, while skinprint quantity may correspond with mass, the relationship between skinprint quality and mass may be more complex. The applicant has identified a need for a rugged, reliable system with low cost consumables for the purpose of analysing skinprint quality/quantity.
Against this background, there is provided a method of determining presence of a skinprint using an apparatus comprising: a primary electromagnetic radiation source; an electromagnetic radiation detector; and a translucent waveguide comprising a first surface providing a waveguide interface coincident with a skinprint receiving region;
In this way, a skinprint may be used to couple electromagnetic radiation into or out of a translucent waveguide. The extent of the coupled electromagnetic radiation is detected or at least inferred and thereby provides an indication of the quality/quantity of the skinprint.
In a further aspect of the disclosure, there is provided an apparatus for determining presence of a skinprint, the apparatus comprising:
Embodiments of the disclosure will now be described, by way of example only, with reference to the accompanying drawing in which:
The disclosure relates to a method and apparatus for determining presence of a skinprint 30. A wide range of alternative implementations is envisaged. The following detailed description relates to a subset of embodiments that fall within the scope of the appended claims.
The translucent waveguide 10 comprises a first end 12 and a second end 14. The LED 40 is optically coupled to the translucent waveguide 10 towards the first end 12.
The second end 14 comprises a fingerprint receiving region 20 on a first surface 16 of the translucent waveguide 10. The fingerprint receiving region 20 may be identified on the first surface 16 by virtue of one or more visible indications on or surrounding the fingerprint receiving region 20. Alternatively, the fingerprint receiving region 20 may be identified by a window bounded by a frame that obscures parts of the first surface 16 that do not form part of the fingerprint receiving region 20. The fingerprint receiving region 20 may be identified by other means.
A surface of the translucent waveguide 10 in the vicinity of the fingerprint receiving region 20 may serve as a waveguide interface 18 through which electromagnetic radiation may be transmitted or in which electromagnetic radiation may be reflected, dependent upon circumstances. The waveguide interface 18 may or may not be different in surface properties when compared to a surface of the translucent waveguide 10 that surrounds the waveguide interface 18.
The photodiode 50 is located so as to detect electromagnetic radiation that is transmitted out of the translucent waveguide 10 via the waveguide interface 18.
The LED 40 is optically coupled to the translucent waveguide 10 towards the first end 12 such that electromagnetic radiation 70 emitted by the LED 40 enters into the translucent waveguide 10 at an angle such that the electromagnetic radiation 70 is retained within the translucent waveguide by total internal reflection. Optical coupling of the LED 40 to the translucent waveguide 10 may take any appropriate form. At the point of entry of the electromagnetic radiation 70 into the translucent waveguide 10, some refraction of the electromagnetic radiation 70 may take place. (For the sake of clarity, this refraction is not shown in the Figures.) In particular, electromagnetic radiation 70 that is incident upon an end surface of the translucent waveguide 10 at an angle of incidence is transmitted into the translucent waveguide 10 with a small change in direction away from a normal line (which is shown in the Figure) that is perpendicular to the surface through which the electromagnetic radiation 70 enters the translucent waveguide 10. The extent of the refraction that takes place depends upon the ratio between the index of refraction of the translucent waveguide 10 and the index of refraction of the material through which the electromagnetic radiation 70 travels immediately prior to reaching the point of entry. Where the material immediately prior to the electromagnetic radiation 70 reaching the point of entry is ambient air, the ratio is likely to be higher (and so the extent of the refraction is likely to be greater) than if the material immediately prior to the electromagnetic radiation 70 reaching the point of entry is, for example, a translucent encapsulation material of an LED package. Accordingly, the nature and extent of any refraction will depend upon how the electromagnetic radiation 70 is coupled from the electromagnetic radiation source 40 into the translucent waveguide 10.
Subsequently, as the electromagnetic radiation 70 travelling within the translucent waveguide 10 reaches the edges of the translucent waveguide 10, it arrives at an angle of incidence that is such as to cause the electromagnetic radiation 70 to reflect at the perimeter of the translucent waveguide 10 as a result of total internal reflection rather than to be transmitted out of the translucent waveguide 10. This pattern of total internal reflection is reproduced along the translucent waveguide 10 and by this mechanism the electromagnetic radiation 70 propagates along and within the translucent waveguide 10.
While in
In the case that no skinprint is present on the skinprint receiving region, as is evident from
By contrast, as can be seen from
In the embodiment of
It should be noted that
Furthermore, it should be noted that the electromagnetic radiation 70 will not all travel in exactly the directions indicated by the arrows in
In the Figures, the schematic representation of a skinprint 30 (where present) is such as to suggest that it is manifested as a single dome-shaped form on the skinprint receiving region 20. It is emphasised that this representation is highly schematic. Again as the skilled person readily appreciates, the form of skinprints varies significantly depending upon many factors including the amount of eccrine sweat on the surface of the skin when printed and the force with which a user places the skin against the skinprint receiving region 20 when providing a skinprint. In reality, the skinprint is likely to comprise a number of peaks and troughs, all of which may influence the behaviour of electromagnetic radiation incident upon it in a variety of ways.
As can be seen from
The applicant has developed various techniques for chemical analysis of skinprints. In order to determine that the chemical analysis is feasible for a given skinprint, it is helpful to have an indication that there is sufficient material present in a skinprint in order to apply a particular chemical test and, in particular, to quantify results of the chemical analysis relative to a mass or volume of the skinprint under test. The techniques described herein provide an indication of the amount of skinprint (hereinafter referred to skinprint quality) that has been deposited on the skinprint receiving region. Where skinprint quality is high, the influence of the skinprint on the behaviour of electromagnetic radiation will be higher than when the skinprint quality is low which will in turn be higher than when there is no skinprint present.
While the techniques described herein may be useful for providing a binary output that simply indicates whether a skinprint is of sufficient quality for a chemical analysis to be performed (by exceeding a fingerprint quality threshold), for skinprints that meet this threshold it may also be desirable to provide a more granular quantitative output. This may in turn be used to provide an indication of a quantum of a particular chemical constituent that may be expected. For example, a high quality skinprint may be expected to contain more of a particular chemical than a lower (but still adequate) quality skinprint. Accordingly, if the subsequent chemical analysis is intended not only to detect for presence of a chemical but also for an indication of concentration of that chemical, a quantitative analysis of the quality of the skinprint may be used in this determination.
The apparatus of the first embodiment may comprise controller circuitry configured to receive the photodiode signal and process that signal in order to determine whether a skinprint quality threshold is met. It may also be configured to determine a metric for quality of the skinprint. The controller may, for example, be configured to receive a first (reference) photodiode signal prior to a user providing a skinprint on the skinprint receiving region and to receive a second photodiode signal once a skinprint has been provided on the skinprint receiving region and to compare the first and second signals. It may also be configured to make a comparison with a reference value indicative of a theoretical maximum that would be achieved in the event of a maximum quality skinprint. By appropriate processing of the first and second signals relative to the reference value a skinprint quality value may be calculated for a particular skinprint and output via a display or as data transmitted for onward processing and/or storage. Alternatively or in addition, it may be that the result is simply compared to a threshold to determine if it meets a predetermined criterion or criteria for a meaningful analysis and a simple binary output may be provided such as a red/green indication (in the manner of traffic lights).
The skilled person will recognise that there are a large number of options applicable to the first embodiment of the disclosure for processing data such as photodiode signals as well as data relating to the electromagnetic radiation emitted by the LED in order to calculate a skinprint quality value. Moreover, in some of the subsequent embodiments that include additional features and functionality, there may be further inputs for calculation of the skinprint quality value. In some or all of these embodiments, there may be a calibration technique as a precursor to performing the analysis that results in the skinprint quality value.
It may be that where a quantitative skinprint quality value is provided, this provides an input to an algorithm related to chemical analysis of the skinprint thereby providing a reference for an amount of chemical that might be expected in a skinprint of that particular quality value. In addition or instead it may be that the skinprint quality value is output to a chemical analysis process simply to confirm that the skinprint is of sufficient quality/quantity to be appropriate for chemical analysis. In this way, it may be possible to avoid the time and expense associated with performing chemical analysis on a skinprint in which there is no confidence that a meaningful chemical analysis can be performed because the quality/quantity of the skinprint is insufficient.
As the skilled person would readily appreciate, alternative embodiments (not illustrated) may involve only one of the two grating couplers 15, 17. For example, an alternative embodiment may include a first grating coupler 15 in the absence of a second grating coupler 17. In such an embodiment electromagnetic radiation 70 that is not transmitted out of the waveguide interface 18 and continues to propagate through the translucent waveguide 10 by total internal reflection may be transmitted (coupled) out of the translucent waveguide 10 in the same manner as in the second and third embodiments 2, 3. Similarly, a further alternative embodiment may include a second grating coupler 17 in the absence of a first grating coupler 15. In such an embodiment, electromagnetic radiation 70 may be coupled into the translucent waveguide 10 in the same manner as for the first, second and third embodiments 1, 2, 3.
Accordingly, when no skinprint is present, only the secondary radiation 75 reaches the photodetector 50. This is because the primary electromagnetic radiation 70 from the primary electromagnetic radiation source 40 is reflected by the waveguide interface 18 rather than being transmitted through it. (A reflector 90 may be used to ensure that the secondary radiation, once out of the waveguide 10, is directed to the photodetector 50.)
When a skinprint 30 is present, primary radiation 70 from the primary radiation source 40 passes through the waveguide interface 18 such that both primary and secondary radiation 70, 75 reach the photodetector 50.
In one aspect of the seventh embodiment 7, one or both of the primary and secondary radiation sources 40, 80 may be pulsed. For example, if the primary radiation source 40 is constant and the secondary radiation source 80 is pulsed then the primary radiation 70 can be detected when the secondary radiation source 80 is off. A value for the secondary radiation 80 can be calculated by subtracting the measured primary radiation 70 from the measured combination of primary and secondary radiation when the secondary radiation source 80 is on.
If the primary and secondary radiation sources 40, 80 are of the same specification (e.g. in terms of brightness and spectrum) then they will both be affected by the material properties of the translucent waveguide 10 in the same way. Accordingly, it is possible by this technique to eliminate variations that arise from the use of different waveguides. This may be particularly appropriate where the waveguide 10 is a consumable product that is replaced with each test performed.
In common with the fifth and sixth embodiments (and by contrast with the first, second, third, fourth and seventh embodiments), the direction of potential transmission through the waveguide interface 18 (in the presence of a skinprint) is into the translucent waveguide 10 rather than out of the translucent waveguide 10.
The primary electromagnetic radiation source 40 is located such that primary electromagnetic radiation 70 reaches the waveguide interface 18 from the exterior of the translucent waveguide 10 towards the first end 12 of the translucent waveguide 10. In addition, the fingerprint receiving region 20 is located on the first surface 16 of the translucent waveguide 10 also towards the first end 12 of the translucent waveguide 10. In the event that a skinprint is present, electromagnetic radiation 70 is transmitted through the waveguide interface 18 and into the translucent waveguide 10 for onward propagation towards the second end 14 of the translucent waveguide 14 through total internal reflection as shown schematically in
The secondary electromagnetic radiation source 80 is located such that secondary radiation 75 is directed into the translucent waveguide 10 at an angle such that it propagates through the translucent waveguide 10 without opportunity for it to be coupled out of the translucent waveguide 10 until it reaches the second end 14 of the translucent waveguide in the region of the photodetector 50. This may be achieved by directing the secondary electromagnetic radiation 75 into the translucent waveguide 10 in a direction that is only marginally angled relative to the first surface 16 of the translucent waveguide 10 (or potentially substantially parallel to the first surface). In this way, the angle of travel of the secondary electromagnetic radiation 75 through the translucent waveguide 10 is such that neither the presence nor the absence of a skinprint 30 enables the radiation to be coupled out of the translucent waveguide 10, at least to any substantial degree.
Electromagnetic radiation (whether primary or secondary) that reaches the second end 14 of the translucent waveguide 10 is detected by the first photodiode 50. In the event that no skinprint 30 is present on the skinprint receiving region 20 (see
As in the seventh embodiment, one or both of the primary and secondary radiation sources 40, 80 may be pulsed. For example, if the primary radiation source 40 is constant and the secondary radiation source 80 is pulsed then the primary radiation 70 can be detected in isolation when the secondary radiation source 80 is off. Where no skinprint 30 is present (such that no primary radiation would be expected to arrive at the photodiode 50) the photodiode would detect radiation only when the secondary radiation source 80 is on.
Alternatively, the secondary radiation source 80 may be constant and the primary radiation source 40 may be pulsed. In this way, where no skinprint is present there should be little difference between the radiation detected by the photodetector 50 regardless of the pulsed nature of the primary radiation 70 since the primary radiation 70 (when on) is not coupled into the translucent waveguide 10 and therefore does not reach the photodetector 50.
If the primary and secondary radiation sources 40, 80 are of the same specification (e.g. in terms of brightness and spectrum) then they will both be affected by the material properties of the translucent waveguide 10 in the same way. Accordingly, it is possible by this technique to eliminate variations that arise from the use of different waveguides. This may be particularly appropriate where the waveguide 10 is a consumable product that is replaced with each test performed.
In any embodiment involving both primary and secondary radiation, as an alternative to the pulsing strategy for separating primary and secondary radiation detected at the photodetector 50, it may be possible to use primary radiation having a different colour from that of the secondary radiation and use a colour sensitive photodetector to distinguish between the primary and secondary radiation. In short, any appropriate technique for distinguishing between primary and secondary radiation may be employed. Such techniques may include separation in the frequency domain, separation in the time domain, and separation in the colour domain. Whichever separation technique may be employed, the concept is to distinguish between primary radiation (main path) and secondary radiation (reference path).
The skilled person will appreciate that aspects of different embodiments described herein may be combined, including in ways not explicitly recited. For example, in the case of the seventh embodiment, it may be appropriate to use two photodiodes, in the manner of embodiments 2, 4, 5 and 6. Similarly, it may be appropriate to use a secondary electromagnetic radiation source in any of embodiments 1 to 6.
The skilled person will understand that refraction necessarily occurs when electromagnetic radiation passes between materials having different refractive indices (unless, of course, the difference of refractive indices is such as to result in total internal reflection). For the sake of clarity only, refraction is not shown in the schematic representations of
The angle of incidence, θi, at which the primary electromagnetic radiation 70 reaches the waveguide interface 18 is necessarily greater than the angle of incidence, θi2, at which the secondary electromagnetic radiation 75 reaches the waveguide interface 18. The exact values for θi and θi2 will depend, among other things, on the refractive indices of the material used for the translucent waveguide 10 and the material (e.g. ambient air) on adjacent the waveguide interface 18 of the translucent waveguide 10.
Where total internal reflection of the primary electromagnetic radiation 70 having the angle of incidence, θi, occurs at the waveguide interface 18 it reflects at an angle of reflection, θr.
While the schematic Figures show the electromagnetic radiation taking only a single path, as the skilled person will readily appreciate, the path of the radiation will diverge. The single lines shown in the Figures are intended to represent the average path of the radiation and for clarity the divergence of radiation from the average path is not shown.
The primary and/or secondary electromagnetic radiation sources may be a source of visible spectrum radiation. The primary and/or secondary light source may be an LED, a filament bulb, a laser, a fluorescent bulb, or any other suitable source of electromagnetic radiation.
The primary and/or secondary electromagnetic radiation may be broad spectrum or narrow spectrum radiation. Potentially, it may be two non-contiguous ranges of narrow spectrum radiation. In some embodiments, the primary and secondary electromagnetic radiation may have the same properties (e.g. wavelength); in other embodiments the primary and secondary electromagnetic radiation may be selected to have different properties (e.g. wavelength).
While the specific embodiments employ one or more photodiodes as electromagnetic radiation detector(s), any appropriate electromagnetic radiation detector(s) may be used. Choice of electromagnetic radiation detector may be dependent, among other things, on the electromagnetic radiation source. Possible electromagnetic radiation detectors include: a photodiode; a phototransistor; a CCD sensor; and a light dependent resistor.
It may be appropriate to use a camera and/or a photomultiplier instead of or in addition to the electromagnetic radiation detector(s) shown in the specific embodiments. In particular, a camera may be used to provide an image of the electromagnetic radiation which may be compared to a database of such images for confirming the identity of a skinprint subject.
While the term skinprint is used throughout this specification, it will be appreciated that the most frequently used form of skinprint is currently the fingerprint (which includes the thumb-print). Nevertheless, other skinprints may be appropriate, such as (but not limited to) a hand-print, a toe-print, a footprint or an ear-print.
The translucent waveguide of any of the embodiments may be any translucent having appropriate properties of transmissivity of electromagnetic radiation of the appropriate wavelengths. The translucent waveguide may be transparent. It may be a glass slide or a plastic slide. An off the shelf slide may be particularly appropriate in embodiments where the translucent waveguide is intended to be a consumable item whereby a new translucent waveguide is employed for each test. If a plastic slide is employed, it may be produced by injection moulding and optionally it may be plasma treated to obtain desirable waveguide properties.
| Number | Date | Country | Kind |
|---|---|---|---|
| 1613819.0 | Aug 2016 | GB | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/GB2017/052365 | 8/10/2017 | WO | 00 |