Patent Grant
PP22079
Latin name: Pinus elliottii.
Variety denomination: ‘CF PS1-3352’.
A new variety of slash pine tree (Pinus elliottii Engelm.) has been discovered. This selection has been designated as ‘CF PS1-3352.’
This new variety is a progeny of two first generation selections. Female parent is a first generation selection made in Taylor County, Fla. Male parent is a first generation selection made in Wayne County, Ga.
Cross pollination occurred in early 1998 followed by induction and cryopreservation of embryogenic tissue in 1999. First somatic seedlings were produced in 2000 and planted in early 2001 in three field experiments. A total of 15 ramets were planted at 5 ramets per field experiment. The field experiments are located in Florida and Georgia.
A new and distinct cultivar of slash pine (Pinus elliottii) is distinctly characterized by high growth rate, good resistance to fusiform rust and pitch canker, excellent stem straightness, medium crown width, long stem internodes, flat to medium branch angle, medium branch diameter and which is mature for commercial harvesting sooner than conventionally grown trees under the ecological conditions prevailing the Atlantic and Gulf Coastal Plains of the United States.
The Pinus elliottii plants of this variety were asexually propagated using an advanced form of micropropagation called somatic embryogenesis carried out at a production facility in Victoria, Canada. Somatic embryogenesis uses a complex process which relies on the splitting of one embryo into many identical embryos. Somatic embryos can then be grown into plants which are all identical genetically. The asexual propagation occurs at an earlier stage in the plant's life cycle than most other micropropagated plants. The detailed methods for somatic embryogenesis used for asexually propagating conifers in general are described in U.S. Pat. No. 6,372,496 and for slash pine in particular in U.S. Patent Application Publication No. 2004/0203150.
The drawings are color photographs showing the new variety of slash pine.
The botanical details of this new and distinct variety of slash pine tree follow. All color descriptions are made in reference to The Royal Horticultural Society (R.H.S.) Colour Chart (2005).
Compared to unimproved slash pine trees, ‘CF PS1-3352’ is characterized by high growth rate, good resistance to fusiform rust (caused by Cronartium quercuum (Berk.) Miyabe ex Shirai f. sp. fusiforme (Cumm.) Burds. et Snow), good resistance to pitch canker (caused by Fusarium circinatum Nirenburg et O'Donnell), excellent stem straightness, medium crown width, long stem internodes, flat to medium branch angle and medium branch diameter.
Descriptions are mean values from five trees measured at age 9.5 years. Branch data is the mean of branches from the whorl immediately above and below a point 8 feet from the base of each tree. Trees were located in Nassau County, Fla. and measurements were recorded on Oct. 31, 2010.
Although the new variety of slash pine tree possesses the detailed characteristics noted above as a result of the growing conditions prevailing in the test locations, it is to be understood that the variations of the usual magnitude and characteristics incident to changes in growing conditions, irrigation, fertilization, pruning, pest control, climatic variations and the like are to be expected. An example of ‘CF PS1-3352’ can be found at Nassau year 2001 line trial, Nassau county, Fla.
Microsatellite markers were used to generate a unique DNA fingerprint for the variety. Young foliage samples from 5 ramets of PS1-3352 variety and from the parental trees used to make the PS1 cross were collected for DNA fingerprinting. The DNA extraction protocol of Doyle and Doyle (1987) was used after slight modifications. DNA fingerprinting of parents and the PS1-3352 variety was conducted using a set of six microsatellite markers (Echt et al., 2006; Echt et al., 2008). Table 1 shows the sequences and conditions for each primer.
Microsatellite products were detected by M13 tailed primer (Oettling et al., 1995). The amplification products were electrophoresed on 5.5% Long Ranger polyacrylamide gels using a LiCor 4200 automated sequencer (LiCor Inc., Lincoln, Nebr.).
The observed parental genotypes and their expected offspring's genotypes at six studied SSR loci of each family are presented in Table 2. PS1-3352 fingerprint based on 6 loci is presented in Table 3.
Auckland, L., T. Bui, Y. Zhou, M. Shepherd and C. Williams. 2002. Conifer Microsatellite Handbook Corporate Press, Raleigh, N.C., USA.
Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh tissue. Phytochemical bulletin 19:11-15.
Echt, C. S., Nelson, C. D., Erpelding, J. E. and Burns, R. 2006. Southern Institute of Forest Genetics, USDA Forest Service Southern Research Station, 23332 Mississippi 67, Saucier, Miss. 39574, USA. On-line genetic database: http://www.ncbi.nlm.nih.gov/unists
Echt, C. S., Saha, S. and Nelson, C. D. 2008. Southern Institute of Forest Genetics, USDA Forest Service Southern Research Station, 23332 Mississippi 67, Saucier, Miss. 39574, USA. On-line genetic database: http://www.ncbi.nlm.nih.gov/unists
Oetting, W. S., H. K. Lee, D. J. Flanders, G. L. Wiesner, T. A. Sellers and R. A. King. 1995. Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared florescence and M13 tailed primers. Genomics 30:450-458.
| Number | Date | Country | |
|---|---|---|---|
| 20100325764 P1 | Dec 2010 | US |
