The present invention relates generally to material transfer devices and, more particularly, to pipettes.
Pipettes and capillary tubes are commonly used to collect and dispense liquids. For example, such devices are particularly useful for collecting blood samples. Known pipettes generally include a draw tube and a squeeze bulb connected to the draw tube. The squeeze bulb is compressed and then released in order to draw a liquid into the draw tube through an opening. The liquid is held within the draw tube as a result of the interior of the pipette exhibiting a lower air pressure than an external atmospheric pressure. The squeeze bulb is then compressed to dispense the liquid from the pipette through the draw tube opening.
Known pipettes exhibit the shortcoming that, upon release of the squeeze bulb after its initial compression, liquid is drawn into the pipette through the draw tube opening at a high flow rate. This often results in the simultaneous drawing of air through the draw tube opening, and thus the formation of air bubbles within the volume of liquid held within the pipette. Such air bubbles undesirably inhibit the ability of the pipette to draw and dispense precise volumes of liquid.
Accordingly, there is a need for improvements to known pipettes to address at least this shortcoming.
A transfer pipette according to an exemplary embodiment of the invention includes a draw tube, a first squeeze bulb, and a second squeeze bulb. The draw tube includes a proximal end, a distal end, and a lumen. The first squeeze bulb defines a first fluid chamber in fluid communication with the lumen at the proximal end, and the second squeeze bulb defines a second fluid chamber in fluid communication with the first fluid chamber. When the first squeeze bulb is squeezed into a compressed state, a volume of air is evacuated from the first fluid chamber. When the first squeeze bulb is released from the compressed state, an intended nominal volume of material is drawn into the draw tube through the distal end. When the second squeeze bulb is compressed, at least a portion of the intended nominal volume of material is dispensed from the draw tube through the distal end.
A kit according to an exemplary embodiment of the invention includes the transfer pipette described above and a fluid absorbent medium adapted to receive thereon at least a portion of the intended nominal volume of material dispensed from the draw tube.
A transfer pipette according to another exemplary embodiment of the invention includes a body having an open end and a closed end, and first and second fluid passageways located between the open and closed ends, the first fluid passageway terminating at the open end. A first squeeze bulb is located between the first and second fluid passageways and defines a first fluid chamber in fluid communication with the fluid passageways. A second squeeze bulb is located between the second fluid passageway and the closed end and defines a second fluid chamber in fluid communication with the first and second fluid passageways. When the first squeeze bulb is squeezed into a compressed state, a volume of air is evacuated from the first fluid chamber. When the first squeeze bulb is released from the compressed state, a predetermined volume of material is drawn into the first fluid passageway through the open end of the body. When the second squeeze bulb is compressed, the predetermined volume of material is dispensed from the first fluid passageway through the open end.
A method of transferring material with a transfer pipette according to an exemplary embodiment of the invention includes compressing a first squeeze bulb of the transfer pipette, and positioning an open end of the transfer pipette in fluid communication with a supply of material. The first squeeze bulb is released from its compressed state to allow an intended nominal volume of the material to be drawn into the transfer pipette through the open end. The method further includes compressing a second squeeze bulb of the transfer pipette to dispense at least a portion of the intended nominal volume of the material from the transfer pipette through the open end.
Various additional features and advantages of the invention will become more apparent to those of ordinary skill in the art upon review of the following detailed description of exemplary embodiments taken in conjunction with the accompanying drawings. The drawings, which are incorporated in and constitute a part of this specification, illustrate one or more exemplary embodiments of the invention and, together with the general description given above and the detailed description given below, serve to explain the exemplary embodiments.
Like reference numerals are used to indicate like features throughout the various figures, wherein:
Referring to
As described in greater detail below, in use, to draw a sample of material into the transfer pipette 10, the first squeeze bulb 18 is fully compressed and then released to slowly draw an intended nominal volume of material, such as a liquid or a powder, into the draw tube 16. Advantageously, the slow rate at which the material is drawn, or aspirated, into the draw tube 16 substantially prevents formation of air bubbles within the drawn material held in the draw tube 16, thereby enabling drawing and dispensing of precise volumes of material. The slow material draw rate, and resultant prevention of bubble formation, is enabled by the simultaneous drawing of air from the second squeeze bulb 20 into the first squeeze bulb 18 while drawing the material into the draw tube 16. The second squeeze bulb 20 then may be at least partially compressed to dispense at least a portion of the intended nominal volume of material from the draw tube 16. Accordingly, the first squeeze bulb 18 is configured to function as an aspiration bulb, and the second squeeze bulb 20 is configured to function as a dispense bulb. The embodiments of the present invention disclosed herein are particularly useful for drawing and dispensing very small volumes of material, for example on the order of microliters (μL) as described below.
As shown in the figures, the first squeeze bulb 18 includes tubular portion 24 having proximal and distal rounded portions 26. Similarly, the second squeeze bulb 20 includes a tubular portion 28, a proximal domed portion 30, and a distal conical portion 32. As used herein, the term “tubular” is not limited to structures having circular cross-sectional shapes. In that regard, as shown and as described in greater detail below, the tubular portion 24 of the first squeeze bulb 18 may be formed with a non-circular or circular shaped cross-section, for example. While the draw tube 16, the first squeeze bulb 18, the connecting tube 22, and the second squeeze bulb 20 are shown arranged in a substantially linear configuration to define a common longitudinal axis, it will be appreciated that various alternative configurations of these components may also be provided while achieving the preferred slow draw of material into the draw tube 16 as described in greater detail below.
A pair of tab-like flanges 34 may extend between the proximal rounded portion 26 of the first squeeze bulb 18 and the distal conical portion 32 of the second squeeze bulb 20. In particular, the flanges 34 may be diametrically opposed and extend along the length of the connecting tube 22 so as to define a plane that intersects a longitudinal axis of the connecting tube 22. Advantageously, the flanges 34 increase the rigidity of the transfer pipette 10 and may be gripped by a user for secure handling of the transfer pipette 10. Additionally, the surfaces of the flanges 34 may be provided with visual indicia for identifying the internal contents and/or an internal volume of the transfer pipette 10, for example. The flanges 34 and the connecting tube 22 may be formed with any suitable length to aid in handling and use of the transfer pipette 10.
Referring to
Referring to
An outer surface of the draw tube 16 may include one or more volume indicating elements, such as graduation marks, between the proximal and distal ends of the draw tube 16, for providing a visual indication of a volume of material contained within the draw tube 16. In the illustrated embodiments, a volume indicating element is shown in the form of an annular rib 46. It will be appreciated that various other forms of volume indicating elements may be provided, such as printed indicia including rings, notches, numbers, letters, symbols, or other markings, for example. Moreover, it will be appreciated that volume indicating elements may be omitted from the draw tube 16 if desired.
A proximal-most one of the volume indicating elements, such as rib or other graduation mark 46, is positioned at a distance from the distal opening 38 of the draw tube 16 that corresponds to a nominal intended volume (also referred to as a draw volume or aspiration volume) of material that is to be drawn into and held within the draw tube lumen 40 when the first squeeze bulb 18 is fully compressed and then released. The first squeeze bulb 18 is “fully compressed” when its oppositely disposed sidewalls 48 substantially contact one another at their inner faces, as shown in
The material holding portion 50 may have an internal volume that is equal to the intended nominal volume of material to be drawn into the transfer pipette 10. The proximal-most volume indicating element or graduation mark 46 may further define a buffer portion 52 of the draw tube 16 located proximally of the proximal-most volume indicating element 46 and having an internal volume intended for holding air rather than drawn material. It will be appreciated that compression of the first squeeze bulb 18 to an extent below which its sidewalls 48 substantially contact one another may result in the drawing of a volume of material less than the intended nominal volume of the material holding portion 50. Additional volume indicating elements (not shown) may be positioned distally of the proximal-most indicating element 46 for indicating predetermined portions of the material holding portion 50 that are less than the intended nominal volume of material to be drawn into the draw tube 16.
In one embodiment, the transfer pipette 10 may be sized, and the proximal-most volume indicating element 46 may be positioned, such that the material holding portion 50 of the draw tube 16 holds a predetermined volume of material of approximately 20 μL to approximately 250 μL. For example, the material holding portion 50 may have an internal volume of approximately 50 μL, 75 μL, 125 μL, or 175 μL, as indicated in the data table shown in
The length of the draw tube 16 may be increased or decreased as desired while maintaining an internal volume of the lumen 40, and thus of the material holding and buffer portions 50, 52, by simultaneously adjusting an inner diameter of the draw tube 16 that defines the lumen 40. For example, the draw tube 16 may be lengthened while simultaneously decreasing the inner diameter, or the draw tube 16 may be shortened while simultaneously increasing the inner diameter. For applications in which the material being drawn is blood or other fluids more viscous than water, the draw tube 16 may be formed with an inner diameter of approximately 0.016 inches to approximately 0.024 inches. Additionally, for such blood applications it may be preferable to form the draw tube 16 with an inner diameter of greater than approximately 0.013 inches to greater than approximately 0.100 inches in order to avoid lysis of red blood cells.
The first squeeze bulb 18 may be generally sized such that a volume of the first fluid chamber 42 is greater than the internal volume of the material holding portion 50 of the draw tube 16. That is, the volume of the first fluid chamber 42 may be greater than the intended volume of material to be drawn in, or aspirated, by the draw tube 16. In various embodiments, the volume of the first fluid chamber 42 may be at least 10% greater than, or up to at least 50% greater than, the internal volume of the material holding portion 50, for example. In certain select cases where a capillary action of the draw tube 16, determined by the inner diameter of the draw tube 16, and a surface tension of the material being drawn interact positively to a sufficient degree, the volume of the first fluid chamber 42 may be equal to or less than the internal volume of the material holding portion 50.
The second squeeze bulb 20 may be generally sized such that a volume of the second fluid chamber 44 is greater than the volume of the first fluid chamber 42, and greater than the internal volume of the material holding portion 50 of the draw tube 16. In one embodiment, the second fluid chamber 44 may be formed with a volume that is at least 1.5 times the internal volume of the material holding portion 50, to provide for easy dispensing of the material held within the draw tube 16 when the second squeeze bulb 20 is compressed, as described in greater detail below.
As shown in
As shown in
The transfer pipette 10 may be integrally formed of any flexible polymeric material suitable for use with powder or liquid materials such as blood or other liquids having more corrosive components. For example, the transfer pipette 10 may be formed of a flexible polymer, such as low density polyethylene (LDPE), linear low density polyethylene (LLDPE), medium density polyethylene (MDPE), high density polyethylene (HDPE), polypropylene (PP), polyvinylidene difluoride (PVDF), fluorinated ethylene propylene (FEP), perfluoralkoxy (PFA), or other suitable polymers with known flexibility in thin wall sections. As described below in connection with
Referring to
As shown in
For many applications, in order to generally maintain the bubble prevention benefit described above, a suitable available volume of the material 54 from which the intended nominal volume of material 54 is to be drawn into the material holding portion 50 is approximately equal to the intended nominal volume to be drawn and at least an additional 30% of material 54. For example, a suitable available volume of the material 54 for use with a pipette having an intended draw volume of 75 μL may be at least 100 μL.
The slow draw capability of the transfer pipettes 10 and 10a is enhanced by the presence of second squeeze bulb 20 in fluid communication with first squeeze bulb 18. In particular, as shown in
As a result, the air directed to the second squeeze bulb 20 causes an increase in internal pressure in the second squeeze bulb 20. When the first squeeze bulb 18 is released to draw the material 54 into the draw tube 16, a portion of the air expelled from first squeeze bulb 18 and directed to second squeeze bulb 20 is returned to first squeeze bulb 18 (as indicated by the arrow shown in second squeeze bulb 20) while the material 54 is drawn into the draw tube 16. This reduces the overall pressure differential between the first squeeze bulb 18 and ambient so that the material 54 is drawn into the draw tube 16 at a slow speed of draw that reduces, or possibly eliminates, the presence of bubbles in the drawn material 54. Once the draw is complete, the pressure is equalized at a value less than atmospheric and the material 54 is retained in the draw tube 16.
As shown in
As shown in
An exemplary application of the setup shown in
In an alternative exemplary blood screening application, the absorbent medium 56 may be in the form of a paper blood test card (not shown), such as those commonly known in the art, rather than a piece of filter paper. The card may be handled without use of a container 58. A face of the card may include indicia defining a plurality of segregated test regions for receiving a respective plurality of blood droplets. In one embodiment, each of the test regions may be pre-impregnated with a respective reactant configured to react with the respective blood droplet to indicate presence or absence of a particular characteristic of the blood droplet.
Alternatively, the transfer pipette 10 may be supplied in bulk to permit transfer of liquid from one container or test tube to another container or test tube, or from a container, a test tube, or a heel or finger prick to a testing device, for example.
Referring to
Referring to
Referring to
The decreased internal volume of the first fluid chamber of pipette 110 causes a smaller volume of air to be expelled from the first squeeze bulb 112 when the bulb 112 is fully compressed, as compared to pipette 10. Consequently, when the first squeeze bulb 112 is released and allowed to return to its relaxed state, a smaller volume of material is drawn into draw tube 16. Accordingly, the proximal-most volume indicating element or graduation mark 46 provided on draw tube 16 is positioned closer to the distal end 14 on pipette 110 than on pipette 10, indicating a material holding portion 50 of decreased volume. It will be appreciated that the first squeeze bulb, or aspiration bulb, of the pipettes disclosed herein may be formed with any suitable length and cross-sectional shape and size for aspirating any suitable nominal intended volume of material.
As shown in
Referring to
As will be appreciated by persons skilled in the art of blow molding, for a parison having a given pre-blown wall thickness, the wall thickness of a bulb blown from the parison is inversely proportional to an outer diameter of the blown bulb. That is, a blown bulb having a smaller outer diameter will have a greater wall thickness than a blown bulb having a larger outer diameter. In the context of exemplary pipette 210, as shown in
As described above, the non-circular shaped cross-section of first squeeze bulb 18 of transfer pipette 10 aids in reducing the rate at which the bulb sidewall 48 rebounds from a compressed state to its relaxed state, thereby contributing to a desirable slower material draw rate than that achieved by known transfer pipettes. Advantageously, a similar slower rebound rate of the first squeeze bulb 212 of pipette 210 is achieved as a result of the increased wall thickness of bulb 212 relative to the wall thickness of bulb 18 of pipette 10. To that end, it will be understood that squeeze bulbs of greater wall thicknesses generally rebound at slower rates than similarly shaped squeeze bulbs of lesser wall thicknesses. Accordingly, it will be appreciated that an aspiration bulb of a transfer pipette in accordance with an embodiment of the invention may be formed with a non-circular shaped cross-section, an increased wall thickness (e.g., by virtue of a decreased outer diameter), or a combination of both in order to achieve a generally slower bulb rebound rate that contributes to a desirable slower material draw rate.
Moreover, in embodiments in which both the aspiration bulb and the dispense bulb are formed with circular cross-sectional shapes and are arranged linearly, as exemplified by transfer pipette 210, the resulting pipette is fully symmetrical circumferentially about a single longitudinal axis. Advantageously, such a configuration may increase ease of manufacture, and thus decrease costs, associated with corresponding blow molds (see, e.g., molds 80, 82 of
Referring to
Second column 304 of table 300 indicates an aspiration volume, measured in μL, for each Embodiment 1-11. This measurement corresponds to an internal volume of the material holding portion of the draw tube of each pipette (see, e.g., material holding portion 50 of draw tube 16 of pipette 10). As shown, these volumes may range from approximately 30 μL to approximately 225 μL, for example.
Third column 306 of table 300 provides a brief description for each Embodiment 1-11 with respect to whether the Embodiment 1-11 includes one, multiple, or no graduation marks (see, e.g., volume indication element or graduation mark 46), and a general cross-sectional shape of the aspiration bulb (e.g., first squeeze bulb 18). Embodiment 7, having an aspiration bulb with a circular cross-sectional shape, may be an exemplary embodiment of the transfer pipette 210 shown in
Fourth column 308 of table 300 indicates an outer diameter, measured in inches, of a draw tube at the distal end of each Embodiment 1-11 (see, e.g., draw tube 16 at distal end 14 of pipette 10).
Fifth column 310 of table 300 indicates a distance, measured in inches, between the distal end of the draw tube and any volume indicating elements or graduation marks provided on the draw tube (see, e.g., distance between distal end 14 of draw tube 16 and volume indicating element 46). For example, Embodiment 6 includes two graduation marks positioned at 0.68 inches and 1.01 inches, respectively, from the distal end of the draw tube.
Sixth column 312 of table 300 indicates a length, measured in inches, of a draw tube of each Embodiment 1-11. This measurement corresponds to the distance between the distal end of the draw tube and the distal end of the aspiration bulb (see, e.g., distance between distal end 14 of draw tube 16 and the distal end of first squeeze bulb 18 of pipette 10). As shown, pipettes with larger aspiration volumes may have longer draw tubes.
Seventh column 314 of table 300 indicates a major axis dimension, a minor axis dimension, and a length, all measured in inches, of the aspiration bulb for those Embodiments in which the aspiration bulb is formed with an oval cross-sectional shape (see, e.g., first squeeze bulb 18 in
Eighth column 316 of table 300 indicates an outer diameter, measured in inches, of the aspiration bulb for those Embodiments in which the aspiration bulb is formed with a circular cross-section; in particular, Embodiment 7.
Ninth column 318 of table 300 indicates an outer diameter, measured in inches, of the circular cross-section of the dispense bulb of each Embodiment 1-11 (see, e.g., second squeeze bulb 20 of pipette 10).
Tenth column 320 of table 300 indicates an overall length, measured in inches, of each Embodiment 1-11 (see, e.g., distance between proximal end 12 and distal end 14 of pipette 10).
While the present invention has been illustrated by the description of specific embodiments thereof, and while the embodiments have been described in considerable detail, it is not intended to restrict or in any way limit the scope of the appended claims to such detail. The various features discussed herein may be used alone or in any combination. Additional advantages and modifications will readily appear to those skilled in the art. The invention in its broader aspects is therefore not limited to the specific details, representative apparatus and methods and illustrative examples shown and described. Accordingly, departures may be made from such details without departing from the scope of the general inventive concept.
This application claims the filing benefits of U.S. Provisional Application Ser. No. 62/202,548 filed Aug. 7, 2015, and U.S. Provisional Application Ser. No. 62/250,578 filed Nov. 4, 2015, each disclosure of each is hereby incorporated by reference herein in its entirety.
Number | Date | Country | |
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62250578 | Nov 2015 | US | |
62202548 | Aug 2015 | US |