SMALL PHYCOBILIPROTEINS FOR USE AS FLUORESCENT MARKERS

Information

• Research Project
• 3498791

• ApplicationId

  3498791

• Core Project Number

  R43GM047766

• Full Project Number

  1R43GM047766-01

• Serial Number

  47766

• FOA Number

• Sub Project Id

• Project Start Date

  3/15/1992 - 32 years ago

• Project End Date

  9/14/1992 - 32 years ago

• Program Officer Name

• Budget Start Date

  3/15/1992 - 32 years ago

• Budget End Date

  9/14/1992 - 32 years ago

• Fiscal Year

  1992

• Support Year

  1

• Suffix

• Award Notice Date

  3/13/1992 - 32 years ago

Organizations

• Martek Biosciences Corporation

Phycobiliproteins are currently being used as fluorescent markers in many aspects of immunodetection of antigens (e.g. flow cytometry). The currently available phycobiliproteins all exceed 100 kDa molecular weight. A whole class of phycobiliproteins have not been used for this purpose, those that are produced by the cryptomonads. These vary in molecular weight, but all are considerably less than 100 kDa. This proposal seeks to explore the utility of these compounds as fluorescent markers. It proposes to culture at least six different cryptomonads that cover the full range of phycobiliproteins produced by this group of algae. These will be lysed and the pigments extracted and purified to homogeneity. The pigments will be characterized as to their spectrophotometric characteristics and molecular weights. They will be conjugated to avidin or streptavidin and the conjugate tested for stability. The stable conjugates will be given to our consultant for testing in flow cytometry experiments.

IC Name

• Activity

  R43

• Administering IC

  GM

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  821

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  SSS

• Study Section Name

• Organization Name

  MARTEK BIOSCIENCE CORPORATION

• Organization Department

• Organization DUNS

• Organization City

  COLUMBIA

• Organization State

  MD

• Organization Country

  UNITED STATES

• Organization Zip Code

  21045

• Organization District

  UNITED STATES