Patent Application
20140289921
This invention pertains to a new and distinct variety of smooth cordgrass.
Smooth cordgrass (Spartina alterniflora Loisel.) is a perennial grass native to intertidal saline marshes along the Atlantic and Gulf of Mexico coasts in the United States. It is used in numerous restoration projects to decrease coastal erosion. In Louisiana, one (1) smooth cordgrass variety, ‘Vermilion’, is used extensively. The widespread use of a single variety reduces genetic variation, thus reducing the ability to adapt to environmental changes. ‘LA12-102’, along with ‘LA11-101’, ‘LA11-102’, ‘LA11-103’, ‘LA12-101’, and ‘LA12-103’, was invented to provide genetically diverse smooth cordgrass varieties for northern Gulf of Mexico restoration projects.
Smooth cordgrass seeds were collected from one hundred twenty-six (126) smooth cordgrass populations throughout Louisiana in 1998. Preliminary work, which preceded this invention, evaluated smooth cordgrass plant survival and vigor in natural marsh environments and freshwater production ponds (Ryan, 2003. http://utils.louislibraries.org/cgi-bin/lz0050.x?sitecode=LALUelib?http://etd.;lsu.edu/docs/available/etd-1110103-133154/ and Ryan et al., 2007. J. Aquat. Plant Manage. 45:90). In the preliminary work, no efforts were made to asexually reproduce ‘LA12-102’ for any purpose other than to provide plant material for plant performance evaluations. The new smooth cordgrass varieties (‘LA12-101’, ‘LA12-102’, ‘LA12-103’, ‘LA11-101’, ‘LA11-102’, and ‘LA11-103’) were designated as varieties and reproduced asexually beginning in 2010. ‘LA12-102’ has a unique and stable genotype, as determined by molecular marker profiles.
‘LA12-102’ is a new smooth cordgrass (Spartina alterniflora Loisel.) variety that is genetically different from ‘Vermilion’ and five (5) newly developed smooth cordgrass varieties, ‘LA12-101’, ‘LA12-103’, ‘LA11-101’, ‘LA11-102’ and ‘LA11-103’. Genetic diversity was determined using thirteen (13) molecular markers. ‘LA12-102’ is more vigorous one (1) month after transplant than ‘Vermilion’ and produces more seeds that germinate than ‘Vermilion’. ‘LA12-102’ is recommended for brackish and saline marsh restoration projects in the northern Gulf of Mexico, especially Louisiana, where genetically different smooth cordgrass varieties that rapidly establish and produce viable seeds are desired.
This new and distinct smooth cordgrass variety, identified as ‘LA12-102’, is characterized by its unique genetic profile, as determined by thirteen (13) molecular markers, ability to recover quickly in natural environments, and high viable seed yields.
The file of this Patent contains at least one photograph executed in color. Copies of this Patent or Patent Application with color drawings(s) will be provided by the US Patent and Trademark Office upon request and payment of the necessary fees.
‘LA12-102’ was developed from the open-pollination of several hundred smooth cordgrass plants that were collected from saline and brackish marshes in the northern Gulf of Mexico. ‘LA12-102’ originated from one (1) seed harvested in October 1998 from a plant originally collected north of the mouth of the Rio Grande River in Texas (2° 57′24.46″ N.; 97° 08′41.50″ W.); the paternal parent is unknown. Seeds were germinated in a greenhouse at Baton Rouge, La. This population was selected in a phenotypic selection program because of high seed germination rates, seedling survival, and seedling vigor. The twenty (20) most vigorous seedlings from this population were selected in a second (2nd) selection cycle and evaluated at Baton Rouge, La. In 1999, ‘LA12-102’, along with thirty-nine (39) additional genotypes, was selected in a third (3rd) selection cycle. To provide plant material for continued experimental evaluations, rhizomes and stems of ‘LA12-102’ were harvested from the experimental plot at Baton Rouge and planted into containers in controlled greenhouses. This asexual reproduction was used solely to multiply the experimental line for evaluation, and not for the asexual reproduction of the variety.
In general, ‘LA12-102’ leaves are slightly darker green [7.5 GY (5/4)] than ‘Vermilion’ [5 GY (5/4)] when color is determined with the MUNSELL® Book of Color (Munsell Color, Gretag Macbeth LLC, 617 Little Britain Road, New Windsor, N.Y. 12553-6148). ‘LA12-102’ stems are red [5 R (6/4)] while stems of ‘Vermilion’ are green [2.5 GY (8/4)] beginning at panicle emergence (
†Means within the same column that are followed by different letters are significantly different (t test, p < 0.05).”
‘LA12-102’ and thirty-nine (39) additional genotypes were evaluated in experiments at Baton Rouge and Grand Terre, La. from 2000-2001. ‘LA12-102’ and seven (7) genotypes were selected in the fourth (4th) selection cycle. In 2002, rhizomes and stems of ‘LA12-102’ were harvested from experimental plots at Baton Rouge and planted into containers in controlled greenhouses. This asexual reproduction was used solely to provide material for continued experimental evaluations.
Experimental evaluations were continued from 2005-2009 at seven (7) sites. In 2010, ‘LA12-102’ was identified as a superior cultivar and one hundred (100) single stems with rhizomes, which were verified using molecular markers to be genetically identical, were used to asexually propagate the variety ‘LA12-102’ in Plaquemines Parish, La. In 2011, rhizome and stem material were harvested and moved to Baton Rouge, La., where asexual reproduction of rhizomes and stems has continued.
Variation in plant appearance can be caused by production conditions and does not reflect genetic differences. ‘LA12-102’ is genetically identical and stable when produced from rhizome material. ‘LA12-102’ can be definitely identified from ‘Vermilion’, ‘LA11-101’, ‘LA11-102’, and ‘LA11-103’ based upon fragment size differences using thirteen (13) molecular markers (Table 2).
†ESSR sequences (Baisakh et al., 2009. Aquat. Bot 91:262; SPAR sequences Blum et al., 2004. Mol. Ecol. Notes 4: 39).
Preliminary Field Trials: ‘LA12-102’ was selected from a preliminary field trial in which four hundred (400) plants were evaluated for performance in a freshwater production pond, Baton Rouge, La., in February 2000. ‘LA12-102’ was included in replicated trials from 2000 to 2009 in thirteen (13) environments to evaluate its performance and release potential.
Advanced Field Trials: Advanced field trials were completed at freshwater ponds, Baton Rouge, La., and on a created marsh, Grand Terre, La., in 2001. The average plant height, spread, rust rating (Puccinia sparganioides Ellis & Tracy), and plant vigor were calculated based upon measurements completed every two (2) weeks starting eight (8) months after transplant and ending twelve (12) months after transplant for Baton Rouge and from three (3) to five (5) months after transplant for Grand Terre. Plant height was measured from the soil surface to the uppermost leaf tip of the plant. Plant spread was calculated by measuring the linear growth of each plant on two (2) perpendicular axes. Rust rating was measured with a 0-10 scale where 0 was no rust visible and 10 was rust covering all above-ground portions of the plant. Plant vigor was measured with a 0-10 scale where 0 was a dead plant and 10 was an extremely vigorous plant.
Elite Field Trials: Elite field trials were completed on a created marsh, Grand Terre; on man-made marsh terraces, Cameron Parish, La., in 2003; and in freshwater rice production fields, Rayne, La., in 2005 and 2006. Plant vigor was measured at Grand Terre and Cameron Parish approximately six (6) months after transplant. Plant vigor, rust rating, plant spread, plant height, and the total number of stems per plot were measured at Rayne, La. in 2005 and 2006, approximately five (5) months after transplant. Percent seed set and seed germination were also determined at Rayne, La. in 2005. Percent seed set was determined on ten (10) randomly selected panicles harvested the first week of November. Each panicle was individually bagged with 4.5 cm×40 cm cellulose tubes to collect seeds that shattered prior to harvest in early December. The total number of florets and the number of florets containing seeds (filled florets) were determined using a fluorescent light box. Percent seed set was calculated as follows: [(number of filled seed/total number of florets)*100]. Percent seed germination was determined with five (5) replicates of one hundred (100) seeds. Seeds were placed into Petri dishes containing a #4 filter paper and 8 ml of 0.05% 200 g/L carboxin and 200 g/L thiram solution. Petri dishes were sealed with parafilm to minimize evaporation and placed in an incubator at 24-26° C. and 16/8 hr light/dark for six (6) weeks and percent seed germination was calculated.
Supreme Field Trials: Supreme field trials were evaluated at a man-made marsh terrace, Cameron Parish, La., in 2008; two (2) eroded marsh areas, Grand Chenier, La., in 2008 and 2009; and two (2) freshwater rice production fields, Rayne, La., in 2008 and 2009. Plant vigor was measured at Cameron Parish one (1) month after transplant. Plant vigor and number of stems were measured at Cameron Parish one (1) year after transplant. Plant vigor, rust rating, plant spread, plant height, and number of stems per plot were measured approximately six (6) months after transplant at Grand Chenier, in 2008 and 2009. Plant vigor, rust rating, plant spread, plant height, number of stems, percent seed set, and percent seed germination were measured at Rayne in 2008 and 2009, approximately six (6) months after transplant.
In advanced field trials, ‘LA12-102’ was as tall, spread as far, and had a similar vigor as ‘Vermilion’, ‘LA11-101’, ‘LA11-102’, and ‘LA11-103’ (Table 3). ‘LA12-102’ had a rust rating that were similar to ‘Vermilion’, ‘LA11-102’, and ‘LA11-103’, but higher than ‘LA11-101’ at Baton Rouge and similar to all varieties at Grand Terre (Table 3).
†Means within the same column that are followed by different letters are significantly different (t test, p < 0.05).
‡Rust rating was visually estimated on a scale of 0-10 (0 = no rust visible; 10 = rust covering all above-ground portions of the plant).
§Vigor was visually estimated on a scale of 0-10 (0 = dead; 10 = excellent).
In elite field trials, ‘LA12-102’ was as vigorous as ‘Vermilion’ at all locations and was more vigorous than ‘LA11-101’ at Rayne in 2005 and ‘LA11-101’, ‘LA11-102’, and ‘LA11-103’ at Rayne in 2006 (Table 4). ‘LA12-102’ had similar rust ratings and vegetative spread as all smooth cordgrass varieties at Rayne in 2005 and 2006 (Table 4). The height of ‘LA12-102’ varied in relation to the other smooth cordgrass varieties based upon location (Table 4). ‘LA12-102’ had more stems than ‘LA11-101’, ‘LA11-102’, and ‘LA11-103’ at all locations and less stems than ‘Vermilion’ at Rayne in 2005 (Table 4). ‘LA12-102’ had a seed set and germination that was similar to ‘LA11-101’, ‘LA11-102’, and ‘LA11-103’, but greater than ‘Vermilion’ (Table 4).
†Vigor was visually estimated on a scale of 0-10 (0 = dead; 10 = excellent);
‡Rust rating was visually estimated on a scale of 0-10 (0 = no rust visible; 10 = rust covering all above-ground portions of the plant).
In supreme field trials, ‘LA12-102’ was more vigorous than ‘Vermilion’ one (1) month after transplant on man-made marsh terraces in Cameron Parish; however, one (1) year after transplant at the same site, ‘LA12-101’ was as vigorous and had approximately an equal number of stems per plot as ‘Vermilion’ (Table 5). ‘LA12-102’ had a similar vigor, rust reaction, plant spread, and number of stems per plot as ‘Vermilion’, ‘LA11-101’, ‘LA11-102’, and ‘LA11-103’ at Grand Chenier and Rayne in 2008 and 2009 (Table 5). The height of ‘LA12-102’ varied across locations and the seed set and seed germination was similar for ‘LA12-102’, ‘LA11-101’, ‘LA11-102’, and ‘LA11-103’, but significantly greater than ‘Vermilion’ at Rayne in 2008 and 2009 (Table 5).
†Vigor was visually estimated on a scale of 0-10 (0 = dead; 10 = excellent);
‡Rust rating was visually estimated on a scale of 0-10 (0 = no rust visible; 10 = rust covering all above-ground portions of the plant).
§Cameron Parish was established in 2008 and evaluated in 2008 one (1) month after transplant and in 2009 one (1) year after transplant.
The development of this invention was partially funded with Hatch Act Formula Funds (Project number LAB93864) and through grants from the United States Department of Agriculture's National Institute of Food and Agriculture (Agreement numbers: 2010-34396-21191, 2009-34396-20051, 2008-34396-19316, 2006-34396-17624, 2005-34396-16498). The Government may have certain rights in this invention.
