Claims
- 1. A bio-sensor for measuring a substrate concentration of a liquid sample, comprising:
- at least a working electrode and a counter electrode,
- an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, and
- a porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
- 2. The bio-sensor according to claim 1, wherein the buffer is a phosphate.
- 3. The bio-sensor according to claim 1, wherein an electron acceptor is carried on the porous substrate in a dry state.
- 4. The bio-sensor according to claim 1 further comprises an oxidizing dye.
- 5. The bio-sensor according to claim 4, wherein the oxidizing dye is a member selected from the group consisting of potassium ferricyanide, p-benzoquinone, 2,6-dichlorophenolindophenol, methylene blue, phenazine methosulfate and potassium beta-naphtoquinone-4-sulfonate.
- 6. The bio-sensor according to claim 1, wherein the oxidoreductase is a member selected from the group consisting of glucose oxidase, alcohol oxidase, xanthine oxidase and cholesterol oxidase.
- 7. The bio-sensor according to claim 1, wherein the oxidoreductase is glucose oxidase.
- 8. The bio-sensor according to claim 1, wherein the working electrode comprises platinum.
- 9. The bio-sensor according to claim 8, wherein the porous plate has a pore diameter of less than 3 micron meters.
- 10. The bio-sensor according to claim 1, further comprises a reference electrode.
- 11. The bio-sensor according to claim 1, wherein the porous plate is a hydrophilic porous membrane.
- 12. The bio-sensor according to claim 1, further comprising a filter having a pore size of less than 3 micron meters positioned and arranged on the porous plate.
- 13. The bio-sensor according to claim 12, wherein an anticoagulant is carried on the filter.
- 14. The bio-sensor according to claim 12, which further comprises a reference electrode.
- 15. The bio-sensor according to claim 1, wherein the counter electrode comprises platinum or silver/silver chloride.
- 16. The bio-sensor according to claim 1, wherein the pore size of the porous plate is between 1000 Angstroms and 1 mm.
- 17. A bio-sensor for measuring a substrate concentration of a liquid sample, which comprises:
- at least a working electrode and a counter electrode,
- an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, and
- a porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state and contacts the electrodes without an intervening electrolyte layer between (i) the oxidoreductase and the buffer and (ii) the electrodes, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
CROSS-REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of application Ser. No. 932,945 filed Nov. 28, 1986, now abandoned, which is a continuation of application Ser. No. 673,751, filed Nov. 8, 1984, now abandoned.
US Referenced Citations (9)
Non-Patent Literature Citations (1)
Entry |
Webster, Medical Instrumentation, Houghton Mifflin Co. (1978), see p. 540 FIG. 10.13. |
Continuations (1)
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673751 |
Nov 1984 |
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Continuation in Parts (1)
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932945 |
Nov 1986 |
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