Patent Application
20120309006
The present invention relates, in general, to prostate cancer. More specifically, the present invention relates to a method to diagnose prostate cancer in a patient by detecting a PCA3 sequence, and more particularly a PCA3 RNA, the PCA3 sequence detected in a sample from the patient being specifically associated with prostate cancer. The invention also relates to kits containing nucleic acid primers and kits containing nucleic acid primers and nucleic acid probes to diagnose, assess, or prognose a human afflicted with prostate cancer.
Over the last decade, cancer of the prostate has become the most commonly diagnosed malignancy among men and the second leading cause of male cancer deaths in the western population, following lung cancer.
Early detection and treatment of prostate cancer before it has spread from the prostate gland reduces the mortality of the disease. This is particularly true for younger men who are at greater risk of dying from this pernicious but slowly growing malignancy. This realization has prompted increasing efforts for early diagnosis and treatment. Indeed, the American Cancer Society and American Urological Association recommend that male population at large undergo annual screening for prostate cancer beginning at age 50. The recommended age for screening is lowered to 40 for men giving a family history of prostate cancer or other risk factors.
With this increasing focus on prostate cancer screening, more men than ever before are being routinely tested for prostate cancer. Not surprisingly, this practice has increased early detection of onset of the disease, as reflected by an apparent increase in the incidence of prostate cancer and decrease in the apparent average age of diagnosis. The clinical hope is that earlier detection of prostate cancer before it metastasizes will reduce the overall mortality rate. Healthcare payers look for early screening and detection to translate into a reduction in the healthcare burden, as early treatment can be less radical, more successful and therefore provided at a lower cost per treated patient. The key to accomplishing this goal remains providing better differential diagnostic tools.
Screening for prostate cancer now involves both palpation of the prostate by digital rectal examination and assay of plasma levels of prostate specific antigen (PSA). PSA is a serine protease produced by the prostatic epithelium that is normally secreted in the seminal fluid to liquefy it. Disruption of the anatomic integrity of the prostate gland can compromise the cellular barriers that normally restrict PSA to within the duct system of the prostate, allowing it to disperse into blood or urine. A number of conditions can result in leakage of PSA into the blood. They include inflammation of the prostate, urinary retention, prostatic infection, benign prostatic hyperplasia, and prostate cancer. Physical manipulation of the prostate can also increase serum PSA levels, but a mild stimulus, such as digital rectal examination (DRE), does not normally increase serum PSA. It is therefore not surprising that screening of serum PSA as an indicator of prostate cancer is not absolutely predictive.
Despite the fact that measure of blood PSA levels can results from a variety of different causes, it is nonetheless the basis for primary screening for prostate cancer. Measurement of total PSA (tPSA) as a diagnostic assay to predict prostate cancer has been in use since 1991. Levels of 4 ng/ml or greater in blood serum are considered abnormal and predictive of prostate cancer. However, the sensitivity of such elevated tPSA levels is only 79%; thus leaving 21% of patients with prostate cancer undetected. The specificity for all tPSA values of 4 ng/ml or greater is very poor. In addition, estimates of specificity for tPSA levels >4.0 ng/ml are reported to be in the rage of 20% to 59%, averaging around 33%. The vast majority of false positives are ultimately shown to be benign prostatic hyperplasia. The specificity is lowest for modestly elevated tPSA, in the low so-called gray zone of 4 to 10 ng/ml. This low level of specificity results in additional more invasive and costly diagnostic procedures, such as transrectal ultrasounds and prostate biopsies. Such tests when unnecessary are also very traumatic for the patient. The psychological impact of being diagnosed as positive until proven as a false positive should not be understated either.
Because of the shortcomings of tPSA, research has been focused on attempting to develop PSA derivatives to increase the sensitivity and specificity of this general diagnostic approach.
One modification is free PSA (fPSA), which was FDA approved in 1998. PSA in serum can be found either in an unbound form or complexed with circulating protease inhibitors, most commonly with alpha-1-antitrypsin (ACT). Clinicians have shown that the proportion of PSA bound to ACT was significantly higher in men with prostate cancer than in unaffected men or those with benign prostate hypertrophy (BPH). As a guideline, if 25% or less of total PSA is free, this is an indicator of possible prostate cancer. The fPSA assay was approved for use in men with tPSA's for 4 to 10 ng/ml. Thus, the fPSA assay was positioned to improve the specificity over that of tPSA alone. However, the predictivity of the fPSA test is not as good in people with really low or really high tPSA levels. Very low tPSA, regardless of measured fPSA, is predictive of not having cancer, while the converse is true with very high tPSA levels. The diagnostic usefulness of fPSA is relatively limited as it can be associated with either BPH or prostate cancer. The use of fPSA in combination with tPSA has been shown to reduce the number of unnecessary biopsies by about 20%.
Clearly, prostate biopsy is the gold standard for confirming prostate cancer. However, even a biopsy is not always 100% certain. The standard is the sextant biopsy where tissue sample collection is guided by transrectal ultrasound. Often the six samples do not detect the cancer and either a second biopsy procedure or more than six samples are required.
Despite the improvements to prostate cancer screening that have come along in the last ten years, there remains a large unmet need in diagnostic sensitivity and specificity, even when these tools are used in combination. Coupling this with the large incidence of prostate cancer and the need for early, accurate detection, the potential of a true differential diagnostic tool is very significant.
A new prostate cancer marker, PCA3, was discovered a few years ago by differential display analysis intended to highlight genes associated with prostate cancer development (U.S. Ser. No. 09/402,713; 09/675,650; 09/996,953; 60/445,436; WO 98/45420; and WO 01/23550). PCA3 is located on chromosome 9 and composed of four exons. It encodes at least four different transcripts which are generated by alternative splicing and polyadenylation. By RT-PCR analysis, PCA3 expression was found to be limited to the prostate and absent in all other tissues, including testis, ovary, breast and bladder. Northern blot analysis showed that PCA3 is highly expressed in the vast majority of prostate cancers examined (47 out of 50) whereas no or very low expression is detected in benign prostate hyperplasia or normal prostate cells from the same patients [Cancer Res., 1999. Dec 1; 59(23):5975-9]. Moreover, a recent study comparing the clinical performance of RNA telomerase RT and RNA PCA3 detection in the case of prostate cancer showed that the PCA3 gene can be considered as a better marker [Cancer Res., 2002. May 1; 62(9):2695-8].
The PCA3 gene is composed of 4 exons (e1-e4) and 3 introns (i1-i3). While PCA3 appears to be recognized as the best prostate-cancer marker ever identified, this specificity has been contested in the literature. For example, Gandini et al. 2003, claim that the prostate-specific expression of PCA3 is restricted to that of exon 4 of the PCA3 gene.
There thus remains a need to clarify the issue of the specificity of the PCA3 marker and provide tools that will specifically identify PCA3 sequences associated with prostate cancer.
The present invention seeks to meet these and other needs.
In view of the fact that advanced prostate cancer remains a life threatening disease reaching a very significant proportion of the male population, there remains a need to provide the most specific, selective, and rapid prostate cancer detection methods and kits.
The present invention seeks to meet these and other needs.
The present description refers to a number of documents, the content of which is herein incorporated by reference in their entirety.
The present invention relates to diagnostic methods and kits which detect prostate cancer in a more specific and selective fashion than the methods and kits of the prior art.
One aim of this invention is to describe a method to detect prostate cancer in a patient and especially from a urine sample by detecting PCA3 RNA which is associated with prostate cancer.
In one particular embodiment, the PCA3 nucleic acid which is identified is associated with a malignant prostatic state and not with a non-malignant prostatic state. In one such particular embodiment, the PCA3 nucleic acid is PCA3 mRNA. In one other embodiment, the sample is any sample from a patient containing a prostatic cell or nucleic acid therefrom in sufficient quantity to allow amplification thereof, and detection thereof. In a particular embodiment, the PCA3 nucleic acid detected is a PCA3 mRNA associated with prostatic cancer and the sample is a urine sample.
In one particular embodiment, the present invention relates to a method to assess prostate cancer in a patient, by detecting in a sample (e.g. urine sample), the presence of PCA3 RNA which does not contain an intron (e.g. i1) between exons 1 and 2. In another embodiment, the PCA3 RNA which is detected is a spliced RNA which lacks an intron (i2) between exons 2 and 3. In yet another embodiment of the present invention, the PCA3 RNA which is detected is a spliced RNA which lacks an intron (i3) between exons 3 and 4. In another yet particular embodiment of the present invention, the PCA3 RNA which is detected is a spliced RNA which lacks introns between exon 1 and exon 3. In a particularly preferred embodiment of the present invention, the PCA3 RNA which is detected is a spliced RNA which lacks introns between exon 1 and exon 4. In a particularly preferred embodiment of the present invention, the PCA3 RNA which is detected is a spliced RNA which lacks at least one intron (e.g. i1 between exons 1 and 2 and/or i3 between exons 3 and 4). In one embodiment, a PCA3 RNA lacking at least a first intron between exons 1 and 2 is specifically targeted and detected. In another embodiment, the PCA3 RNA which is detected lacks an intron (i3) between exons 3 and 4. In yet another particular embodiment, the detected PCA3 sequence is an intron-less PCA3 RNA.
In one particular embodiment of the present invention, the prostate cancer specific RNA encoded by the PCA3 gene (i.e. RNA) is detected using an amplification method which amplifies a second prostate-specific sequence (which does not have to be associated with prostate cancer) also contained in the sample. A number of such second prostate-specific sequences can be used as long as they can serve as a control for prostate RNA. Non-limiting examples of such prostate-specific sequences include PSA (in this case a prostate-specific sequence often associated with prostate cancer), and other kallikrein family members. The amplification of the prostate-cancer specific PCA3 RNA sequences and the prostate-specific sequences can be carried out simultaneously. The amplification of PCA3 and second prostate-specific sequence can be carried out in the same or in different reaction mixtures and simultaneously or not.
The invention provides a method of detecting prostate cancer-specific PCA3 RNA in a sample.
In one further embodiment, the present invention relates to a method (and kits therefor) to diagnose or prognose prostate cancer in a patient, comprising a detection of a prostate cancer specific PCA3 RNA from the patient, and wherein the PCA3 RNA is associated with a presence of prostate cancer or predisposition thereto in the patient. In one particular embodiment, the PCA3 RNA lacks at least one intron. Numerous sensitive methods of detection of nucleic acids are known, and can be adapted in accordance with the type of sample, the sensitivity of the detection, the amount of PCA3 nucleic acid, and the like. In one particular embodiment, the detection is effected with a probe designed from the sequences shown herein. In one particular embodiment, the probe spans an exon-exon junction of said PCA3 RNA. In yet a further embodiment, the sensitivity of the method (and kit therefor) is increased by further performing an amplification of the PCA3 nucleic acid. Numerous amplification methods can be used. Many of them are described herein. In one particular embodiment, PCA3 nucleic acid, and preferably PCA3 RNA is amplified using a primer which hybridizes to an exonic sequence thereof. In yet a further embodiment, the detection of PCA3 RNA is a detection of an RNA lacking more than one intron. In a further embodiment, which is usually carried out, but not necessarily after an amplification, one or more probe which is used to detect the PCA3 nucleic acid targets an exon-exon junction. Kits enabling such methods are also within the scope of the present invention. The permutations of priming through different exon-junctions, of probing one or more exon junction, or detecting PCA3 RNA lacking a certain intron, more than one, etc, is taught herein. In addition, methods to increase sensitivity, to capture the nucleic acid, and the like, are also taught or exemplified herein.
The invention also provides kits for detecting the presence of prostate cancer-specific PCA3 RNA in a sample. In one embodiment, the invention provides a diagnostic kit comprising at least a first container means containing a pair of primers which can amplify the above-described prostate cancer-specific PCA3 nucleic acid (e.g. RNA). In another embodiment, the invention provides a diagnostic kit containing a first container containing a pair of primers which can amplify the above mentioned prostate specific PCA3 RNA and a second container means containing a pair of primers which can amplify the above-mentioned second prostate-specific sequence. In another embodiment, a third container means contains a probe which specifically hybridizes to the PCA3 amplification product. In a particular embodiment of the invention, the probe further increases the specificity of the method, by specifically hybridizing to a chosen exon-exon junction of PCA3. In yet another embodiment, a fourth container means contains a probe for another region of PCA3 and/or for the second prostate-specific sequence. In yet a further embodiment, the kit comprises reagents to increase the sensitivity of the detection. In one embodiment, the kit contains reagents enabling real-time amplification and detection.
The invention thus further provides a method of diagnosing the presence or predisposition to develop prostate cancer in a patient. The presentation invention prodides methods of diagnosis and prognosis, for in vitro, ex vivo and in vivo use.
In another embodiment, the RNA encoded by the PCA3 gene is obtained from a cell contained in a voided urine sample from the patient.
In one embodiment of the present invention, the RNA is detected using an RNA amplification method. In one such embodiment, the RNA amplification method is coupled to real-time detection of the amplified products using fluorescence specific probes. In yet a further embodiment, the amplification method is PCR. In an additional embodiment the PCR is real-time PCR or a related method enabling a detection in real-time of the amplified products.
In one embodiment, the urine sample is obtained after an attentive digital rectal examination (DRE). Of course, it should be understood that the present methods and kits could also be used on a urine sample obtained without digital rectal examination, or on other types of samples such as sperm, mixed urine and sperm (first urine sample following ejaculation), provided that the amplification method and/or detection method is sensitive enough to detect the targeted markers (PCA3 and when desired the second marker). Experiments showed that the methods and kits of the present invention could also be performed with these types of samples. Other samples which could be used include blood or serum.
In one embodiment, the cells collected from the urine sample are harvested and a total nucleic acid extraction is carried out. In one particular embodiment, total nucleic acid extraction is carried out using a solid phase band method on silica beads as described by BOOM et al. Of course, it should be understood that numerous nucleic acid extraction methods exists and thus, that other methods could be used in accordance with the present invention. One non-limiting example is a phenol/chloroform extraction method. Other such methods are described in herein referenced textbooks.
In one additional embodiment RNA encoded by PCA3 gene is detected by an in vitro RNA amplification method named Nucleic Acid based Amplification (NASBA). Of course other RNA amplification methods are known and the instant methods and kits are therefore not limited to NASBA. Non-limiting examples of such RNA amplification methods include polymerase chain reaction (PCR), transcriptase mediated amplification (TMA) and ligase chain reaction (LCR).
In one embodiment, the amplified products are detected in homogenous phase using a fluorescent probe using the Beacon approach. In another embodiment, the product is detected on solid phase using fluorescent or colorimetric method. It should be understood that numerous fluorescent, colorimetric or enzymatic methods could be used in accordance with the present invention to detect and/or quantify the targeted RNAs. Such fluorescent, colorimetric or enzymatic methods are well known in the art.
It should be understood by a person of ordinary skill that numerous statistical methods can be used in the context of the present invention to determine if the test is positive or negative.
Further objects and advantages of the present invention will be clear from the description that follows.
In the description that follows, a number of terms used in DNA technology are extensively utilized. In order to provide a clear and consistent understanding of the specification and claims, including the scope to be given such terms, the following definitions are provided.
Unless defined otherwise, the scientific and technological terms and nomenclature used herein have the same meaning as commonly understood by a person of ordinary skill to which this invention pertains. Commonly understood definitions of molecular biology terms can be found for example in Dictionary of Microbiology and Molecular Biology, 2nd ed. (Singleton et al., 1994, John Wiley & Sons, New York, N.Y.), The Harper Collins Dictionary of Biology (Hale & Marham, 1991, Harper Perennial, New York, N.Y.), Rieger et al., Glossary of genetics: Classical and molecular, 5th edition, Springer-Verlag, New-York, 1991; Alberts et al., Molecular Biology of the Cell, 4th edition, Garland science, New-York, 2002; and, Lewin, Genes VII, Oxford University Press, New-York, 2000. Generally, the procedures of cell cultures, infection, molecular biology methods and the like are common methods used in the art. Such standard techniques can be found in reference manuals such as for example Sambrook et al. (2000, Molecular Cloning—A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratories); and Ausubel et al. (1994, Current Protocols in Molecular Biology, John Wiley & Sons, New-York).
The use of the word “a” or “an” when used in conjunction with the term “comprising” in the claims and/or the specification may mean “one” but it is also consistent with the meaning of “one or more”, “at least one”, and “one or more than one”.
Throughout this application, the term “about” is used to indicate that a value includes the standard deviation of error for the device or method being employed to determine the value. Routinely a 10% to 15% deviation preferably 10% is within the scope of the term “about”.
Isolated Nucleic Acid Molecule. An “isolated nucleic acid molecule”, as is generally understood and used herein, refers to a polymer of nucleotides, and includes but should not be limited to DNA and RNA. The “isolated” nucleic acid molecule is purified from its natural in vivo state.
DNA Segment. A DNA segment, as is generally understood and used herein, refers to a molecule comprising a linear stretch of nucleotides wherein the nucleotides are present in a sequence that can encode, through the genetic code, a molecule comprising a linear sequence of amino acid residues that is referred to as a protein, a protein fragment or a polypeptide.
Gene. A DNA sequence related to a single polypeptide chain or protein, and as used herein includes the 5′ and 3′ untranslated ends. The polypeptide can be encoded by a full-length sequence or any portion of the coding sequence, so long as the functional activity of the protein is retained.
Complementary DNA (cDNA). Recombinant nucleic acid molecules synthesized by reverse transcription of messenger RNA (“RNA”).
Structural Gene. A DNA sequence that is transcribed into RNA that is then translated into a sequence of amino acids characteristic of a specific polypeptide.
Agarose Gel Electrophoresis. The most commonly used technique (though not the only one) for fractionating double strand DNA is agarose gel electrophoresis. The principle of this method is that DNA molecules migrate through the gel as though it were a sieve that retards the movement of the largest molecules to the greatest extent and the movement of the smallest molecules to the least extent. Note that the smaller the DNA fragment, the greater the mobility under electrophoresis in the agarose gel.
The DNA fragments fractionated by agarose gel electrophoresis can be visualized directly by a staining procedure if the number of fragments included in the pattern is small. In order to visualize a small subset of these fragments, a methodology referred to as the Southern hybridization procedure can be applied.
Southern Transfer Procedure. The purpose of the Southern transfer procedure (also referred to as blotting) is to physically transfer DNA fractionated by agarose gel electrophoresis onto a nitrocellulose filter paper or another appropriate surface or method, while retaining the relative positions of DNA fragments resulting from the fractionation procedure. The methodology used to accomplish the transfer from agarose gel to nitrocellulose involves drawing the DNA from the gel into the nitrocellulose paper by capillary action.
Nucleic Acid Hybridization. Nucleic acid hybridization depends on the principle that two single-stranded nucleic acid molecules that have complementary base sequences will reform the thermodynamically favored double-stranded structure if they are mixed under the proper conditions. The double-stranded structure will be formed between two complementary single-stranded nucleic acids even if one is immobilized on a nitrocellulose filter. In the Southern hybridization procedure, the latter situation occurs. As noted previously, the DNA of the individual to be tested is digested with a restriction endonuclease, fractionated by agarose gel electrophoresis, converted to the single-stranded form, and transferred to nitrocellulose paper, making it available for reannealing to the hybridization probe. Examples of hybridization conditions can be found in Ausubel, F. M. et al. Current protocols in Molecular Biology, John Wily & Sons, Inc., New York, N.Y. (1989). A nitrocellulose filter is incubated overnight at 42° C. with labeled probe in a solution containing 50% formamide, (or at 68° C. without formamide) high salt (either 5× SSC[20×: 3M NaCl/0.3M trisodium citrate] or 5× SSPE [20×: 3.6M NaCl/0.2M NaH2PO4/0.02M EDTA, pH 7.7]), 5×Denhardt's solution, 1% SDS, and 100 μg/ml denatured salmon sperm DNA. This is followed by several washes in 0.2×SSC/0.1% SDS at a temperature selected based on the desired stringency: room temperature (low stringency), 42° C. (moderate stringency) or 68° C. (high stringency). The temperature selected is determined based on the melting temperature (Tm) of the DNA hybrid. Formamide can also be used in the washings and the temperature is adapted in accordance with the desired Tm.
Hybridization Probe. To visualize a particular DNA sequence in the Southern hybridization procedure (e.g. an amplification product), a labeled DNA molecule or hybridization probe is reacted to the fractionated DNA bound to the nitrocellulose filter. The areas on the filter that carry DNA sequences complementary to the labeled DNA probe become labeled themselves as a consequence of the re-annealing reaction. The areas of the filter that exhibit such labeling are visualized. The hybridization probe is generally produced by molecular cloning of a specific DNA sequence. In one particular embodiment the probe spans the 3′ region of a first exon and the 5′ region of a second exon, such that such a probe can only detect the amplification product if the first exon and second exon have been spliced into a contiguous position (i.e. by removing an intervening intronic sequence). Knowing the sequences of the exon boundaries, as well as those of the different exons (see below), the numerous primers and probes which can be designed and used in the context of the present invention can be readily determined by a person of ordinary skill in the art to which the present invention pertains.
Oligonucleotide, Oligomer or oligo. A molecule comprised of two or more deoxyribonucleotides or ribonucleotides, preferably more than three. Its exact size will depend on many factors, which in turn depend on the ultimate function or use of the oligonucleotide. An oligonucleotide can be derived synthetically or by cloning. Chimeras of deoxyribonucleotides and ribonucleotides may also be within the scope of the present invention.
Sequence Amplification. A method for generating large amounts of a target sequence. In general, one or more amplification primers are annealed to a nucleic acid sequence. Using appropriate enzymes, sequences found adjacent to, or in between the primers are amplified.
Amplification Primer. An oligonucleotide which is capable of annealing adjacent to a target sequence and serving as an initiation point for DNA synthesis when placed under conditions in which synthesis of a primer extension product which is complementary to a nucleic acid strand is initiated.
Antisense nucleic acid molecule. An “antisense nucleic acid molecule” refers herein to a molecule capable of forming a stable duplex or triplex with a portion of its targeted nucleic acid sequence (DNA or RNA). The design and modification of antisense nucleic acid molecules is well known in the art as described for example in WO 96/32966, WO 96/11266, WO 94/15646, WO 93/08845, and U.S. Pat. No. 5,593,974. Antisense nucleic acid molecules, as sense oligos, can be derived from the nucleic acid sequences of the present invention and modified in accordance to well known methods. For example, some antisense molecules (or sense oligos or sequences) can be designed to be more resistant to degradation, or if required, to increase their affinity to their targeted sequence, to affect their transport to chosen cell types or cell compartments, and/or to enhance their lipid solubility by using nucleotide analogs and/or substituting chosen chemical fragments thereof, as commonly known in the art. PCA3 gene has also been described as DD3PCA3, the sequence of which is also found as GenBank's accession number AF103907.
Having thus generally described the invention, reference will now be made to the accompanying drawings, showing by way of illustration a preferred embodiment thereof, and in which:
Forward primers:
BUS1 (AGAAGCTGGCATCAGAAAAA, SEQ ID No:12; exon 1, pos. 23-42); AH1 (CAGGAAGCACAAAAGGAAGC, SEQ ID NO:3; exon 3, pos. 443-462); BUS10 (ATCCCTGGGAGAAATGCC, SEQ ID NO:42; exon 4a, pos. 469-486); BUS17 (CACACAGCATGATCATTACGG, SEQ ID NO:43; exon 4b, pos. 1129-1149);
Reverse primers:
BUS7 (CTGGAAATGTGCAAAAACAT, SEQ ID NO:44; exon 3, pos. 420-401); AH2 (TCCTGCCCATCCTTTAAGG, SEQ ID NO:4; exon 4a, pos. 593-575); BUS11 (GTTGCATGTCTTGTGAAGCC, SEQ ID NO:45; exon 4a, pos. 719-700); BUS16 (TGATGGTGATGACAGATAAGGC, SEQ ID NO:46; exon 4b, pos. 1482-1461). The following tissues were analyzed: lane 1, H2O; lane 2, seminal vesicle; lane 3, heart; lane 4, spleen; lane 5, lung; lane 6, bladder; lane 7, bladder-RT; lane 8, prostate cancer; lane 9, normal prostate; lane 10, prostate cancer; lane 11, normal prostate; and lane 12, LNCaP (cell line).
Other objects, advantages and features of the present invention will become more apparent upon reading of the following non-restrictive description of preferred embodiments with reference to the accompanying drawings which are exemplary and should not be interpreted as limiting the scope of the present invention.
Isolated nucleic acid molecules of the present invention are meant to include those that result from any known method, such as chemically synthesized. Similarly, an oligomer which corresponds to the nucleic acid molecule, or to each of the divided fragments, can be synthesized. Such synthetic oligonucleotides can be prepared, for example, by the triester method of Matteucci et al., J. Am. Chem. Soc. 103:3185-3191 (1981) or by using an automated DNA synthesizer.
An oligonucleotide can be derived synthetically or by cloning. If necessary, the 5-ends of the oligomers can be phosphorylated using T4 polynucleotide kinase. Kinasing of single strands prior to annealing or for labeling can be achieved using an excess of the enzyme. If kinasing is for the labeling of probe, the ATP can contain high specific activity radioisotopes. Then, the DNA oligomer can be subjected to annealing and ligation with T4 ligase or the like.
The present invention relates to a nucleic acid for the specific detection, in a sample, of the presence of PCA3 nucleic acid sequences which are associated with prostate cancer, comprising the above-described nucleic acid molecules or at least a fragment thereof which binds under stringent conditions to PCA3 nucleic acid.
In one preferred embodiment, the present invention relates to oligomers which specifically target and enable amplification (i.e. primers) of PCA3 RNA sequences associated with prostate cancer.
In one embodiment, the amplified product can be detected following hybridizing with a probe which consists of an isolated nucleic acid consisting of 10 to 1000 nucleotides (prefererably, 10 to 500, 10 to 100, 10 to 50, 10 to 35, 20 to 1000, 20 to 500, 20 to 100, 20 to 50, or 20 to 35) which hybridizes preferentially to an amplified product which originated from PCA3 RNA associated with prostate cancer, but preferentially not the PCA3 gene, wherein said nucleic acid probe is or is complementary to a nucleotide sequence consisting of at least 10 consecutive nucleotides (preferably, 15, 18, 20, 25, or 30) from the nucleic acid molecule comprising a polynucleotide sequence at least 90% identical to a sequence selected from the group consisting of:
(a) a region of the nucleotide sequence of PCA3 SEQ ID NO:1 or 2, which is associated with prostate cancer;
(b) a nucleotide sequence which spans two exon junctions, preferably exons 1 and 2, exons 1 and 3 (exon 1 being contiguous to exon 3, through alternative splicing), and exons 3 and 4;
(c) a nucleotide sequence which spans a sufficient number of the PCA3 exon junctions, wherein the exon junctions are defined as follows: exon junction of exons 1 and 2, nucleotide positions 98-99 as set forth in SEQ ID NO:1; exon junction of exons 2 and 3, nucleotide positions 263-264 as set forth in SEQ ID NO:1; exon junction of exons 3 and 4a, nucleotide positions 446-447 as set forth in SEQ ID NO:1; and exon junction of exons 4a and 4b, nucleotide positions 985-986 as set forth in SEQ ID NO:1;
(d) a nucleotide sequence which spans a sufficient number of the PCA3 exon junctions, wherein the exon junctions are defined as follows: exon junction of exons 1 and 2, nucleotide positions 120-121 as set forth in SEQ ID NO:2; exon junction of exons 2 and 3, nucleotide positions 285-286 as set forth in SEQ ID NO:2; exon junction of exons 3 and 4a, nucleotide positions 468-469 as set forth in SEQ ID NO:2; exon junction of exons 4a and 4b, nucleotide positions 1007-1008 as set forth in SEQ ID NO:2; exon junction of exons 4b and 4c, nucleotide positions 2066-2067 as set forth in SEQ ID NO:2; and exon junction of exons 4c and 4d, nucleotide positions 2622-2623 as set forth in SEQ ID NO:2.
Preferably, a probe in accordance with the present invention does not specifically hybridize to nucleotides 511-985 of SEQ ID NO:1, to nucleotides 567-961 of SEQ ID NO:1, to nucleotides 533-1007 of SEQ ID NO:2, or to nucleotides 589-983 of SEQ ID NO:2.
Complementary sequences are also known as antisense nucleic acids when they comprise sequences which are complementary to the coding strand. Herein, SEQ ID NOs: 1 and 2 are arbitrarily defined as being the coding strands.
Primers in accordance with the present invention can be designed as commonly known in the art based on the sequences of PCA3 provided herein. More preferably, the primers will be chosen to amplify a PCA3 RNA which is associated with prostate cancer. One such PCA3 RNA is a PCA3 RNA which lacks intron 1 (between exons 1 and 2). Another prostate-cancer specific PCA3 RNA in accordance with the present invention, lacks the intron between exon 3 and exon 4a. Of course different permutations of such prostate-cancer specific PCA3 RNAs are also encompassed by the present invention. For example, three non-limiting prostate-cancer specific PCA3 RNAs include a PCA3 RNA lacking at least intron 1, and PCA3 RNAs having the following contiguous exons: exons 1, 2, 3, 4a, 4b, 4c and 4d; and exons 1, 3, 4a, 4b, 4c and 4d.
In a preferred embodiment of the present invention, a primer which is designed to bind to exon 1 is used, together with a second primer designed to bind to exon 3 or to exon 4. Since intron 1 is a large intron (approximately 20 kb), the amplifying conditions can be selected so as to inhibit the production of such a large amplification product, should the intron be present in the PCA3 sequence. Alternatively, the conditions of amplification can be selected so as to enable the amplification of such large products. In such an embodiment, the presence of intron 1 in the PCA3 RNA can be ascertained by numerous means known in the art (including using an intronic probe and/or a probe which designed to bind to contiguous exon 1-exon 2 sequences; two non-limiting examples thereof is shown in Table 1). It will be recognized by the person of ordinary skill that the position of the primer at the exon junction and the length of the primer can be varied, as known in the art.
In another preferred embodiment, a primer which is designed to bind to exon 1 is used, together with a second primer designed to bind an exon junction region of the present invention. Since exon 1 has been shown to be one preferred targeted exon to amplify prostate-cancer specific RNAs, such an embodiment is especially preferred since it can generate prostate cancer specific amplification products. However, targeting another exon of PCA3 is also another preferred embodiment. For example, exon 3 and exon 4 can be targeted (see below).
Examples of nucleic acid primers which can be derived from the exon sequences shown hereinbelow and specific primers designed to amplify an exon junction of the present invention are set forth in Table 1, below.
While the present invention can be carried out without the use of a probe which targets PCA3 sequences, and preferably the exon junctions of PCA3 in accordance with the present invention, such probes can add a further specificity to the methods and kits of the present invention. Non limiting examples of specific nucleic acid probes which can be used in the present invention (and designed based on the exonic sequences shown in Table 1) are set forth in Table 2, below.
Of course, as will be understood by the person of ordinary skill, a multitude of additional probes can be designed from the same or other region of SEQ ID NO:1 as well as from SEQ ID NO:2 and other sequences of the present invention, whether they target exon junctions or not.
The hybridization probes of the present invention can be labeled by standard labeling techniques such as with a radiolabel, enzyme label, fluorescent label, biotin-avidin label, chemiluminescence, and the like. After hybridization, the probes can be visualized using known methods.
The nucleic acid probes of the present invention include RNA, as well as DNA probes, such probes being generated using techniques known in the art.
In one embodiment of the above described method, a nucleic acid probe is immobilized on a solid support. Examples of such solid supports include, but are not limited to, plastics such as polycarbonate, complex carbohydrates such as agarose and sepharose, and acrylic resins, such as polyacrylamide and latex beads. Techniques for coupling nucleic acid probes to such solid supports are well known in the art.
The test samples suitable for nucleic acid probing methods of the present invention include, for example, cells or nucleic acid extracts of cells, or biological fluids. The sample used in the above-described methods will vary based on the assay format, the detection method and the nature of the tissues, cells or extracts to be assayed. Methods for preparing nucleic acid extracts of cells are well known in the art and can be readily adapted in order to obtain a sample which is compatible with the method utilized. Preferably the sample is a urine sample.
In another embodiment, the present invention relates to a method of detecting the presence of prostate cancer specific PCA3 nucleic acid in a sample comprising a) contacting the sample with the above-described nucleic acid primers, under specific amplification conditions, and b) detecting the presence of the amplified product. One skilled in the art would select the nucleic acid primers according to techniques known in the art as described above. In one particular embodiment one of the primers binds to exon 1 of PCA3. In another embodiment, exon 3 or exon 4 of PCA3 is targeted by the primer. In another embodiment a probe is used to identify the amplification product. Samples to be tested include but should not be limited to RNA samples from human tissue.
In another embodiment, the present invention relates to a kit for detecting the presence of prostate cancer specific PCA3 nucleic acid in a sample comprising at least one container means having disposed therein at least one primer pair, (e.g. one binding to exon 1, the other to exon 3; one binding to exon 1, the other to exon 4a; and one binding to exon 1, the other to exon3-exon4a junction). In a preferred embodiment, the kit further comprises other containers comprising one or more of the following: amplification reagents, probes, wash reagents and reagents capable of detecting the presence of bound nucleic acid probe. Examples of detection reagents include, but are not limited to radiolabelled probes, enzymatic labeled probes (horse radish peroxidase, alkaline phosphatase), and affinity labeled probes (biotin, avidin, or steptavidin).
In detail, a compartmentalized kit includes any kit in which reagents are contained in separate containers. Such containers include small glass containers, plastic containers or strips of plastic or paper. Such containers allow the efficient transfer of reagents from one compartment to another compartment such that the samples and reagents are not cross-contaminated and the agents or solutions of each container can be added in a quantitative fashion from one compartment to another. Such containers will include a container which will accept the test sample, a container which contains the probe or primers used in the assay, containers which contain wash reagents (such as phosphate buffered saline, Tris-buffers, and the like), and containers which contain the reagents used to detect the hybridized probe, bound antibody, amplified product, or the like.
One skilled in the art will readily recognize that the nucleic acid probes described in the present invention can readily be incorporated into one of the established kit formats which are well known in the art.
It is to be understood that although the following discussion is specifically directed to human patients, the teachings are also applicable to any animal that expresses PCA3.
The diagnostic and screening methods of the invention are especially useful for a patient suspected of being at risk for developing a disease associated with an altered expression level of PCA3 based on family history, or a patient in which it is desired to diagnose a PCA3-related disease (ex. prostate cancer).
According to the invention, presymptomatic screening of an individual in need of such screening is now possible using DNA encoding the PCA3 protein or the PCA3 gene of the invention or fragments thereof. The screening method of the invention allows a presymptomatic diagnosis, including prenatal diagnosis, of the presence of a missing or aberrant PCA3 gene in individuals, and thus an opinion concerning the likelihood that such individual would develop or has developed a PCA3-associated disease. This is especially valuable for the identification of carriers of altered or missing PCA3 genes, for example, from individuals with a family history of a PCA3-associated disease (e.g. prostate cancer, urogenital cancer). Early diagnosis is also desired to maximize appropriate timely intervention.
In one preferred embodiment of the method of screening, a tissue sample would be taken from such individual, and screened for the presence of prostate cancer-specific PCA3 nucleic acid.
More specifically, a method of diagnosing the presence or predisposition to develop prostate cancer in a patient is provided herein.
The screening and diagnostic methods of the invention do not require that the entire PCA3 sequence be used for the probe. Rather, it is only necessary to use a fragment or length of nucleic acid that is sufficient to detect the presence of the PCA3 nucleic acid from a normal or affected individual, the absence of such nucleic acid, or an altered structure of such nucleic acid (such as an aberrant splicing pattern). Preferably, any of the probes as described herein are used.
Non-limiting embodiments of the present invention also include an assay for detecting PCA3 RNA in which sequences in the spliced RNA are amplified and detected by using a probe that specifically hybridizes to a chosen exon-exon junction of PCA3 that is in the amplified nucleic acid. Generally, one primer in the amplification reaction hybridizes specifically to a sequence in a first exon (or upstream exon) and the other primer used in the amplification reaction hybridizes specifically to a sequence in a second exon (or downstream exon), and the probe hybridizes to a sequence that spans the 3′ region of the first exon and the 5′ region of the second exon. That is, the probe is specific for a chosen exon-exon junction in an amplified sequence made from a spliced PCA3 RNA that lacks at least one intron between the upstream and downstream exon sequences to which the primers hybridize. Primers for use in amplifying sequences of the spliced RNA that contain a chosen exon-exon junction can readily be determined by using standard methods, so long as the region amplified by the primer pair contains the exon-exon junction sequence or its complementary sequence. Any method of nucleic acid amplification may be used to amplify the sequence that contains the chosen exon-exon junction and procedures for using any of a variety of well-known amplification methods can readily be determined by those skilled in the art.
Probes that detect a chosen exon-exon junction may be labeled with any of a variety of labels that can, directly or indirectly, result in a signal when the probe is hybridized to the amplified sequence that contains the exon-exon junction. For example, a label may be any moiety that produces a luminescent, fluorescent, radioactive, or enzymatic signal that can be detected by using methods well known in the art. A probe need not be labeled with a label moiety if binding of the probe specifically to the amplified nucleic acid containing the exon-exon junction results in a detectable signal, such as, for example a detectable electrical impulse. Non-limiting examples of amplification primers for exon sequences flanking a chosen exon-exon junction and probes specific for some PCA3 junctions are shown herein and exemplified hereinbelow (e.g. Table 3), and are representative of non-limiting embodiments of an assay to detect spliced PCA3 RNA in a sample. Additional exemplary details of procedures that may be used in such assays are described in Examples 4 and 5.
The present invention is described in further detail in the following non-limiting examples.
Gandini et al. 2003 claim that the prostate-specific expression of PCA3 is restricted to exon 4 of the PCA3 gene. The authors show that RT-PCR amplification of the PCA3 transcript using primers specific for exons 1 and 3 also amplified a PCA3-specific product in several non-prostate tissues and cell lines. After the first description of the PCA3 gene, the exon 1 forward and exon 3 reverse PCR primers were used exactly as described in the letter by Gandini et al. supra. In the past four years, PCA3 was amplified in many samples using these primers, and non-prostatic expression of PCA3 has yet to be observed. Although it is not clear from the letter how many cycles of PCR amplification Gandini et al. supra performed, more than 35 rounds of amplification were never used for the results described in this example. It cannot be excluded that using more rounds of amplification might result in detection of low levels of expression. These levels of expression, however, would be far below those observed in prostate cancer, normal prostate and even prostate cancer cell lines.
One interesting observation made herein is that PCA3 can be amplified in non-prostatic tissues, using PCA3-specific primers spanning exons 3 and 4 (
Several explanations for the presence of non-spliced PCA3 transcripts can be postulated (
With respect to
This example illustrates some non-limiting embodiments of the assay for detecting PCA3 RNA in which sequences in the spliced RNA are amplified and detected by using a probe that specifically hybridizes to a chosen exon-exon junction of PCA3 that is in the amplified nucleic acid. Generally, one primer in the amplification reaction hybridizes specifically to a sequence in a first exon (or upstream exon) and the other primer used in the amplification reaction hybridizes specifically to a sequence in a second exon (or downstream exon), and the probe hybridizes to a sequence that spans the 3′ region of the first exon and the 5′ region of the second exon. That is, the probe is specific for a chosen exon-exon junction in an amplified sequence made from a spliced PCA3 RNA that lacks at least one intron between the upstream and downstream exon sequences to which the primers hybridize. Primers for use in amplifying sequences of the spliced RNA that contain a chosen exon-exon junction can readily be determined by using standard methods, so long as the region amplified by the primer pair contains the exon-exon junction sequence or its complementary sequence. Any method of nucleic acid amplification may be used to amplify the sequence that contains the chosen exon-exon junction and procedures for using any of a variety of well-known amplification methods can readily be determined by those skilled in the art.
Probes that detect a chosen exon-exon junction may be labeled with any of a variety of labels that can, directly or indirectly, result in a signal when the probe is hybridized to the amplified sequence that contains the exon-exon junction. For example, a label may be any moiety that produces a colorimetric, luminescent, fluorescent, radioactive, or enzymatic signal that can be detected by using methods well known in the art. A probe need not be labeled with a label moiety if binding of the probe specifically to the amplified nucleic acid containing the exon-exon junction results in a detectable signal, such as, for example a detectable electrical impulse.
Examples of amplification primer pair combinations that amplify nucleic acid sequence that includes an exon-exon junction and embodiments of some exon-exon junction probe sequences are shown in Table 3. It will be understood by those skilled in the art that the probe sequences shown below also include the complementary sequences of the sequences shown, and sequences that include insignificant changes to the specific sequences shown (i.e., the changes do not affect the ability of a probe to hybridize specifically to the chosen exon-exon junction sequence, under standard hybridization conditions). Furthermore, although the probe sequences are shown as DNA sequences, those skilled in the art will understand that the corresponding RNA sequences or their complementary sequences may be used as probes. Also, the backbone linkages of the probe base sequences may include one or more standard RNA linkages, DNA linkages, mixed RNA-DNA linkages, or other linkages such as 2′-O-methyl linkages or peptide nucleic acid linkages, all of which are well known to those skilled in the art.
As shown in Table 3 (first column), the chosen exon-exon junction to be detected may join exons 1 and 2 (exon 1/exon 2), exons 1 and 3 (exon 1/exon 3), exons 2 and 3 (exon 2/exon 3), or exons 3 and 4 (exon 3/exon 4). Primer pairs are sequences located in two different exons that directly or indirectly flank the chosen exon-exon junction (Table 3, second column). Thus, for an exon 1/exon 2 junction, the primer pairs are one primer specific for a sequence contained in exon 1 and another primer specific for a sequence contained in exon 2. But for detecting an exon 2/exon 3 junction or an exon 3/exon 4 junction, the primer pairs may be selected from more than two different exons (see below in column 2) so long as the amplified sequence contains the chosen exon-exon junction region. The “exon 4” primers include primers specific for a sequence contained in any sequence of exons 4a, 4b, 4c, or 4d.
These non-limiting examples of amplification primers for exon sequences flanking a chosen exon-exon junction and probes specific for some PCA3 junctions are representative of embodiments of the assay to detect spliced PCA3 RNA in a sample.
Additional details of procedures and embodiments that may be used in such assays are described in the examples that follow. Those skilled in the art will appreciate that variations of these procedures and reagents may be used that would not materially affect the results or conclusions drawn from these experiments and, therefore, these examples describe non limiting embodiments of the invention.
This example demonstrates the sensitivity of an amplification assay targeting the exon3-exon4 spliced variant of the PCA3 mRNA. For use in these experiments, oligonucleotides were synthesized using standard phosphoramidite chemistry (e.g., as described in Caruthers et al., Methods in Enzymol., 154:287 (1987)), performed by using an automated system (Expedite™ 8909 Nucleic Acid Synthesizer, Applied Biosystems, Foster City, Calif.).
Nucleic acid primers were designed for use in transcription mediated amplification (TMA), a procedure disclosed in detail previously (e.g., Kacian et al., U.S. Pat. Nos. 5,399,491 and 5,480,784). TMA is an isothermal amplification procedure that produces more than a billion-fold increase in copy number of the target sequence by using reverse transcriptase and RNA polymerase. Briefly, in TMA a single-stranded target sequence is used to synthesize a double-stranded DNA intermediate by using reverse transcriptase in the presence of a pair of amplification oligonucleotides, one of which has a 5′ RNA polymerase promoter sequence. The DNA intermediate includes a double-stranded promoter sequence that is recognized by a RNA polymerase and directs transcription of the target sequence (i.e., hundreds of copies of RNA). Each RNA transcript is then converted to a double-stranded DNA intermediate which is used to produce additional RNA and, thus, the reaction proceeds exponentially.
The primers were synthesized with the following sequences:
AATTTAATACGACTCACTATAGGGAGAGGCTCATCGATGACCCAAG
The underlined 5′ portion of the second primer (called a “promoter primer”) is a T7 promoter sequence (AATTTAATACGACTCACTATAGGGAGA, SEQ ID NO:37)
which is used in the TMA procedure, but those skilled in the art will appreciate that a primer made up of the 3′ target-specific sequence (GGCTCATCGATGACCCAAGATGG, SEQ ID NO:38) or its complementary sequence could equivalently be used in an amplification reaction that does not involve T7 RNA polymerase transcription. These primers were designed to amplify across the splice junction between exons 3 and 4 of the PCA3 mRNA.
A probe (GGAAGCACAGAGATCCCTGG, SEQ ID NO:8) was used which spans the exon3-exon4 splice junction. That is, the probe was designed to specifically detect only amplicon derived from that spliced mRNA, and not the exon3-intron3-exon4 unspliced form. The probe was synthesized in vitro to include a non-nucleotide linker (see Arnold et al., U.S. Pat. Nos. 5,585,481 and 5,639,604), and labeled with a chemiluminescent acridinium ester (see Arnold et al., U.S. Pat. No. 5,185,439). The RNA target for amplification was an in vitro transcript that contained the sequence of the exon1-exon3-exon4 spliced form of the PCA3 mRNA. Skilled artisans will appreciate that the target could be produced by other standard methods, such as, e.g., chemical lysis of cells by using a detergent-containing buffered solution that inhibits RNAse activity, including target purification (e.g., as described by Weisburg et al., in U.S. Pat. No. 6,110,678).
Three sample tubes were used for each of the target levels tested (n=3), in the range of 0 to 10,000 copies of target per reaction (see Table 4). To each reaction tube, Amplification Reagent (75 μL of a solution containing 26.7 mM rATP, 5.0 mM rCTP, 33.3 mM rGTP and 5.0 mM rUTP, 125 mM HEPES, 8% (w/v) trehalose dihydrate, 1.33 mM dATP, 1.33 mM dCTP, 1.33 mM dGTP, 1.33 mM dTTP, 33 mM KCl, 30.6 mM MgCl2, 0.10% (w/v) methyl paraben, 0.02% (w/v) propyl paraben, and 0.003% phenol red, at pH 7.5) containing these primers (15 pmol each) was added. Target RNA transcript was then added to the tubes in 10 μL of water, and mixed, and then each reaction tubes received 200 μL of silicone oil (United Chemical Technologies, Inc., Bristol, Pa.), was covered and vortexed for about 10 seconds before being incubated in a 62° C. water bath for about 10 minutes for an initial annealing step in which binding of the promoter-primer to the target nucleic acid occurred. Reaction tubes were then incubated at 42° C. for about 5 minutes, and then 25 μL of the Enzyme Reagent (50 mM HEPES, 125 mM N-acetyl-L-cysteine, 120 mM KCl, 1 mM EDTA, 20% (v/v) glycerol, 10.2% (v/v) TRITON X-100, 0.2 M trehalose, 0.90 U/mL Moloney murine leukemia virus (MMLV) reverse transcriptase (RT), and 0.20 U/mL T7 RNA polymerase, at pH 7.0, wherein 1 U of RT activity is defined as synthesis and release of 5.75 fmol cDNA in 15 min at 37° C. for MMLV RT, and 1 U of T7 RNA polymerase activity is defined as production of 5.0 fmol RNA transcript in 20 min at 37° C.) was added to each reaction tube, mixed gently and incubated at 42° C. for about 60 minutes.
For detection of PCA3 amplification products, the reaction tubes were removed to room temperature and 100 μL of the hybridization reagent (100 mM succinate, 2% (w/v) lithium lauryl sulfate, 100 mM lithium hydroxide, 15 mM aldrithiol-2, 1.2 M LiCl, 20 mM EDTA, 3.0% (v/v) ethanol, at pH 4.7) containing 100 fmol of a labeled detection probe and 400 pmol of unlabeled probe was added to each reaction tube. The labeled detection probe had an acridinium ester label joined to the probe by a non-nucleotide linker positioned between nucleotides 10 and 11, and the unlabeled probe was an oligomer of the same nucleic acid sequence but was not labeled. The reaction tubes were covered and vortexed for about 10 seconds and then incubated at 62° C. for about 20 minutes to allow hybridization of the detection probe to amplification products. Reaction tubes were cooled at room temperature for about 5 minutes and then 250 μL of the Selection Reagent (600 mM boric acid, 182.5 mM NaOH, 1% (v/v) TRITON X-100, at pH 8.5) was added to each tube. Reaction tubes were covered, vortexed for about 10 seconds, and then incubated at 62° C. for about 10 minutes to hydrolyze acridinium ester labels associated with unhybridized labeled probe. Reaction tubes were cooled to about 18° to 28° C. for about 15 minutes and the chemiluminescent signal (in relative light units, or RLU) was detected by using a luminometer (LEADER7 450h or LEADER7 HC+ Luminometer, Gen-Probe Inc., San Diego, Calif.) equipped for automatic injection of Detection Reagent 1 (1 mM nitric acid, 32 mM hydrogen peroxide), followed by automatic injection of Detection Reagent 2 (1.5 M NaOH to adjust the pH to approximately neutral). The cut-off level for a negative result in this experiment was 50,000 RLU, i.e., positive samples provided a signal greater than 50,000 RLU.
The results shown in Table 4 show that the PCA3 assay detects a minimum of 10 copies of the PCA3 RNA transcript. The signal (RLU) obtained using 10 copies of the target was about 15-fold greater than the signal obtained for a negative sample (0 copies). The RLU signals detected correlated positively with the relative amount of target present in the samples, indicating the quantitative nature of this PCA3 assay system.
This example demonstrates detection of the PCA3 exon3-exon4 spliced variant mRNA in cell pellets from urine. Unless otherwise specified, the reagents used were as described in Example 4. Cell pellets were prepared by centrifugation (1500 RCF for 15 min) of 200 μL of urine from each of three normal males and one normal female. Five replicates were prepared and analyzed from each subject. The urine supernatant was decanted, and the tubes drained. gEach cell pellet was lysed in 400 μL of Lysis Buffer (15 mM sodium phosphate monobasic, 15 mM sodium phosphate dibasic, 1.0 mM EDTA, 1.0 mM EGTA, 110 mM lithium lauryl sulfate, at pH 6.7, incubated 10 min at 62° C.) to release target nucleic acids, and then cooled at room temperature for about 5 minutes.
To separate PCA3 mRNA target nucleic acid from other components present in the sample tubes, the contents of the sample tubes were transferred to clean tubes and combined with 100 μL of a Target Capture Reagent (250 mM HEPES, 310 mM LiOH, 1.88 M LiCl, 100 mM EDTA, at pH 6.4, and 250 μg/ml 1 micron magnetic particles (Sera-Mag™ MG-CM Carboxylate Modified, Seradyn, Inc., Indianapolis, Ind.) having (dT)14 oligomers covalently bound thereto) containing 1.5 pmol of a target capture probe (AUCUGUUUUCCUGCCCAUCCUUUAAGTTT(A)30, SEQ ID NO:39). This capture probe includes a 5′ target binding region (AUCUGUUUUCCUGCCCAUCCUUUAAG, SEQ ID NO:40) and a 3′ immobilized probe binding region (TTT(A)30, SEQ ID NO:41). The tubes were covered, hand-shaken, incubated at 62° C. for about 30 minutes to permit hybridization of the target binding region of the capture probe to the target nucleic acid, and cooled at room temperature for about 30 minutes for hybridization of the (dA)30 sequence of the immobilized probe binding region of a complementary capture probe of (dT)14 bound to magnetic particles. Then a magnetic field was applied for about 5 minutes to the outside of the tubes to isolate the magnetic particles with the bound nucleic acids, after which the sample solutions were aspirated from the tubes, and then the particles with bound nucleic acids in each tube were washed with a 1 mL of a Wash Solution (150 mM NaCl, 10 mM HEPES, 6.5 mM NaOH, 1 mM EDTA, 0.3% (v/v) ethanol, 0.1% (w/v) SDS, 0.02% (w/v) methylparaben, 0.01% (w/v) propylparaben, at pH 7.5, by vortexing for 10-20 sec), and again separated using a magnetic field (about 5 minutes) before the Wash Solution was aspirated away from the particles.
Following the target capture step, 75 μL of the Amplification Reagent containing primers described in Example 4 was added to each of the reaction tubes and then amplification, probe hybridization and detection were carried out as described in Example 4. The results shown in Table 5 indicate that the PCA3 assay amplified and detected PCA3-derived nucleic acid from male urine cell pellets, but not from female urine cell pellets. The range of RLU values obtained from the three normal males varied, indicating a difference in recovery of cells expressing PCA3, or a difference in PC3 mRNA expression. The RLU value obtained from the female urine cell pellet was at background, indicating that no PCA3 mRNA was detectable. The term “CV” in Table 5 stands for coefficient of variation and represents the standard deviation of the replicates over the mean of the replicates as a percentage.
All publications mentioned herein are hereby incorporated in their entirety by reference.
Although the present invention has been described hereinabove by way of preferred embodiments thereof, it can be modified, without departing from the spirit and nature of the subject invention as defined in the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2,432,365 | Jun 2003 | CA | national |
This application is a divisional application of U.S. patent application Ser. No. 12/249,519, now pending, which is a continuation application of U.S. patent application Ser. No. 10/880,425, now abandoned. The patent applications identified above are incorporated here by reference in their entirety to provide continuity of disclosure.
| Number | Date | Country | |
|---|---|---|---|
| Parent | 12249519 | Oct 2008 | US |
| Child | 13565592 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 10880425 | Jun 2004 | US |
| Child | 12249519 | US |
