Claims
- 1. An isolated nucleic acid comprising a promoter having a sequence that is at least 85% identical to SEQ ID NO:1 and that includes a 12 nucleotide site having at least 10 nucleotides identical to SEQ ID NO:5.
- 2. The nucleic acid of claim 1 wherein the sequence is at least 95% identical to SEQ ID NO:1:
- 3. The nucleic acid of claim 2 wherein the sequence is SEQ ID NO:1.
- 4. The nucleic acid of claim 1 further comprising a heterologous sequence, wherein a portion of the nucleic acid containing SEQ ID NO:1 directs transcription of the heterologous sequence.
- 5. An isolated nucleic acid comprising SEQ ID NO:2, or a fragment of SEQ ID NO:2 at least 25 nucleotides in length.
- 6. An isolated nucleic acid comprising a promoter that hybridizes under stringent conditions to a nucleic acid consisting of SEQ ID NO:2.
- 7. The isolated nucleic acid of claim 6 further comprising a heterologous sequence, wherein the promoter directs transcription of the heterologous sequence.
- 8. The isolated nucleic acid of claim 7 wherein the heterologous sequence encodes a polypeptide.
- 9. The isolated nucleic acid of claim 8 wherein the polypeptide is selected from the group consisting of a protease inhibitor, collagenase, chitinase, glucanase, glycosidase, and Bacillus thuringiensis δ-endotoxin.
- 10. An isolated nucleic acid comprising a promoter that is at least 85% identical to SEQ ID NO:2.
- 11. The nucleic acid of claim 1, wherein the promoter directs transcription in response to wounding.
- 12. The isolated nucleic acid of claim 7, wherein the promoter directs transcription in response to wounding.
- 13. A vector comprising the nucleic acid of claim 1.
- 14. A vector comprising the nucleic acid of claim 6.
- 15. A transformed cell comprising the nucleic acid of claim 4.
- 16. A transformed cell comprising the nucleic acid of claim 7.
- 17. A transgenic plant whose genome comprises the nucleic acid sequence of claim 4.
- 18. The transgenic plant of claim 17 wherein the plant is a dicot.
- 19. The transgenic plant of claim 17 wherein the plant is selected from the group consisting of Ipomoea batatas, Arabidopsis, rice, tobacco, maize, Brassica, and potato.
- 20. A method of protecting a plant from a pathogen or pest, the method comprising:
transforming a plant cell with the nucleic acid of claim 6; and cultivating the cell to produce a plant.
- 21. A method of producing a polypeptide, the method comprising:
providing a plant having a transgene comprising a promoter having the sequence of the nucleic acid of claim 6, and a heterologous sequence encoding the polypeptide, the heterologous sequence being operably linked to the promoter, harvesting a tissue from the plant; inducing transcription of the heterologous sequence in the tissue by wounding, pathogen infection, pest infestation, or elicitor treatment; and recovering the polypeptide from the harvested plant tissue; to thereby produce the polypeptide.
- 22. A method of producing a polypeptide, the method comprising:
providing a tuberous plant having a transgene comprising a promoter having a sequence of the nucleic acid of claim 1, and a heterologous sequence encoding the polypeptide, the heterologous sequence being operably linked to the promoter; inducing transcription of the heterologous sequence in a tissue by wounding, pathogen infection, pest infestation, or elicitor treatment; harvesting the tissue; and recovering the polypeptide from the tissue; to thereby produce the polypeptide.
- 23. A method of producing a polypeptide, the method comprising:
providing a plant having a transgene comprising a promoter having the sequence of a nucleic acid of claim 1, and directs expression in tubers, and a heterologous sequence encoding the polypeptide, the heterologous sequence being operably linked to the promoter; harvesting tubers from the plant; and recovering the polypeptide from the tissue; to thereby produce the polypeptide.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] Pursuant to 35 USC § 119(e), this application claims the benefit of prior U.S. provisional application 60/289,630, filed May 8, 2001.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60289630 |
May 2001 |
US |