Patent Application
20210199666
Herein is reported an improved setup of a bridging assay for the determination of the biological activity of multispecific, multivalent, e.g. bispecific, bivalent, molecules in which the specificity and robustness of the assay in the presence of oligomeric side-products is increased.
Assays for determining the biological activity of monospecific antibodies are well known. Different assay setups are known, such as e.g.
A biological activity assay is an analytical procedure for measuring the biological activity of a test substance based on a specific, functional biological response of a test system. Biological activity may be assessed using animal based in-vivo assays, cell based in-vitro assays or (receptor) binding assays.
Surface plasmon resonance (SPR) is a well know technology for the determination of antibody-antigen interactions of monospecific antibodies. The technology can be used for the determination of binding affinities of antibodies to their respective antigens.
In WO 2011/117329 bispecific, bivalent anti-VEGF/anti-ANG2 antibodies are reported.
In WO 2009/080251 bivalent, bispecific antibodies are reported. Bispecific antibodies are reported in US 2011/0293613. In WO 2010/040508 bispecific anti-VEGF/anti-ANG2 antibodies are reported Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies is reported by Schaefer, W., et al. (Proc. Natl. Acad. Sci. USA 108 (2011) 11187-11192).
It has been found that assays for determining the biological activity using a general principle as outlined above and not taking into account the findings as reported herein are sensitive to “functional” multimers, i.e. dimers, trimers, tetramers and higher order aggregates, of multivalent, multispecific antibodies, such as bivalent, bispecific antibodies, due to avidity based binding of the multimers.
One aspect as reported herein is the (in vitro) use of the binding site of a multivalent, multispecific antibody that has the smaller (lower) kD value (dissociation constant) for the interaction with its antigen for the immobilization of the multispecific, multivalent antibody to a solid surface for the determination of the biological activity, i.e. functional binding to an antigen, of the multivalent, multispecific antibody.
In one embodiment the determination of the biological activity is by determining the (bridging) binding signal with the antigen for which the multivalent, multispecific antibody has the bigger (higher) kD value (dissociation constant).
In one embodiment the biological activity is the binding affinity.
In one embodiment the determination of the (bridging) binding signal is by surface plasmon resonance or ELISA.
In one embodiment the multivalent, multispecific antibody is selected from a bivalent, bispecific antibody, a trivalent, trispecific antibody, a tetravalent, tetraspecific antibody, a trivalent, bispecific antibody, and a tetravalent, trispecific antibody. In one embodiment the multivalent, multispecific antibody is a bivalent, bispecific antibody.
One aspect as reported herein is the use of the simultaneous binding of a bivalent, bispecific antibody to its first and second antigen in a surface plasmon resonance or ELISA method, whereby the first antigen is immobilized as capture reagent on a solid surface, and whereby the bivalent, bispecific antibody has a kD value for the interaction with the first antigen that is smaller than the kD value for the interaction with the second antigen, for reducing the interference of functional multimeric forms of the bivalent, bispecific antibody in the surface plasmon resonance or ELISA method.
In one embodiment the solid surface is a surface plasmon resonance chip.
In one embodiment the first antigen is a dimer or trimer or tetramer.
In one embodiment functional multimer is a functional dimer.
In one preferred embodiment the first antigen is a dimer or trimer or tetramer and the functional multimer is a functional dimer.
In one embodiment the kD value of the binding site that has the higher kD value for the interaction with its antigen is at least 1.1 times higher than the kD value of the binding site of the bivalent, bispecific antibody that has the smaller kD value.
In one embodiment the kD value is at least 10 times higher.
In one embodiment the kD value is at least 100 times higher.
One aspect as reported herein is the (in vitro) use of the (bridging signal of the) simultaneous binding of a multivalent, multispecific antibody to its first and second antigen for the determination of the biological activity, i.e. functional binding to an antigen, of the multivalent, multispecific antibody,
In one embodiment the multivalent, multispecific antibody is selected from a bivalent, bispecific antibody, a trivalent, trispecific antibody, a tetravalent, tetraspecific antibody, a trivalent, bispecific antibody, and a tetravalent, trispecific antibody. In one embodiment the multivalent, multispecific antibody is a bivalent, bispecific antibody.
In one embodiment the determination of the (bridging) binding signal is by surface plasmon resonance.
One aspect as reported herein is the (in vitro) use of the simultaneous binding of a multivalent, multispecific antibody to its first and second antigen for the determination of the biological activity, i.e. simultaneous functional binding to the first and second antigen, of the multivalent, multispecific antibody to reduce interference from multimeric forms of the multivalent, multispecific antibody,
In one embodiment the multivalent, multispecific antibody is selected from a bivalent, bispecific antibody, a trivalent, trispecific antibody, a tetravalent, tetraspecific antibody, a trivalent, bispecific antibody, and a tetravalent, trispecific antibody. In one embodiment the multivalent, multispecific antibody is a bivalent, bispecific antibody.
In one embodiment the determination of the (bridging) binding signal is by surface plasmon resonance.
In one embodiment of all previous aspects and embodiment the determining of the binding signal for the simultaneous binding of the multivalent, multispecific antibody to its first and second antigen is based on the binding of the bivalent, bispecific antibody to its second antigen.
In one embodiment of all previous aspects and embodiments the solid surface is a surface plasmon resonance chip or the wall of a well of a multi-well plate.
In one embodiment of all previous aspects and embodiments the kD value (dissociation constant) of the binding site that has the bigger (higher) kD value (dissociation constant) for the interaction with its antigen is at least 1.1 times bigger (higher) than the kD value (dissociation constant) of the binding site of the multivalent, multispecific antibody that has the smaller (lower) kD value (dissociation constant). In one embodiment the kD value (dissociation constant) is at least 1.5 times bigger (higher). In one embodiment the kD value (dissociation constant) is at least 2 times bigger (higher). In one embodiment the kD value (dissociation constant) is at least 10 times bigger (higher). In one embodiment the kD value (dissociation constant) is at least 100 times bigger (higher).
In one embodiment of all previous aspects and embodiments the first antigen is a dimer or trimer or tetramer or prone to aggregation.
In one embodiment of all previous aspects and embodiments the functional multimer is a functional dimer.
One aspect as reported herein is an (in vitro) method for determining the biological activity, i.e. functional binding to an antigen, of a multivalent, multispecific antibody comprising the step of:
In one embodiment the determination of the (bridging) binding signal is by surface plasmon resonance.
In one embodiment the multivalent, multispecific antibody is selected from a bivalent, bispecific antibody, a trivalent, trispecific antibody, a tetravalent, tetraspecific antibody, a trivalent, bispecific antibody, and a tetravalent, trispecific antibody. In one embodiment the multivalent, multispecific antibody is a bivalent, bispecific antibody.
One aspect as reported herein is an (in vitro) method for reducing the interference from functional multimeric forms of a multivalent, multispecific antibody in the determination of the biological activity, i.e. functional binding, of the multivalent, multispecific antibody comprising the step of:
In one embodiment the determination of the (bridging) binding signal is by surface plasmon resonance.
In one embodiment the multivalent, multispecific antibody is selected from a bivalent, bispecific antibody, a trivalent, trispecific antibody, a tetravalent, tetraspecific antibody, a trivalent, bispecific antibody, and a tetravalent, trispecific antibody. In one embodiment the multivalent, multispecific antibody is a bivalent, bispecific antibody.
One aspect as reported herein is an (in vitro) method for determining the presence of functional multimers of a multivalent, multispecific antibody in a sample, comprising the step of
In one embodiment the determination of the (bridging) binding signal is by surface plasmon resonance.
In one embodiment the multivalent, multispecific antibody is selected from a bivalent, bispecific antibody, a trivalent, trispecific antibody, a tetravalent, tetraspecific antibody, a trivalent, bispecific antibody, and a tetravalent, trispecific antibody. In one embodiment the multivalent, multispecific antibody is a bivalent, bispecific antibody.
In one embodiment of all previous aspects and embodiments that the determining the binding signal for the simultaneous binding of the bivalent, bispecific antibody to its first and second antigen is based on the binding of the bivalent, bispecific antibody to its second antigen.
In one embodiment of all previous aspects and embodiments the solid surface is a surface plasmon resonance chip.
In one embodiment of all previous aspects and embodiments the first antigen is a dimer or trimer or tetramer or prone to aggregation.
In one embodiment of all previous aspects and embodiments the functional multimer is a functional dimer.
It has been found that assays for determining the biological activity using a principle not taking into account the findings as reported herein are sensitive to “functional” multimers, i.e. functional dimers, functional trimers, functional tetramers and functional higher order aggregates, of multivalent, multispecific antibodies due to avidity based binding of the multimers.
The term “antibody” herein is used in the broadest sense and encompasses various antibody structures, including but not limited to monoclonal antibodies and multispecific antibodies (e.g., bispecific antibodies).
The “class” of an antibody refers to the type of constant domain or constant region possessed by its heavy chain. There are five major classes of antibodies: IgA, IgD, IgE, IgG, and IgM, and several of these may be further divided into subclasses (isotypes), e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. The heavy chain constant domains that correspond to the different classes of immunoglobulins are called α, δ, ε, γ, and μ, respectively.
The term “monoclonal antibody” as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical and/or bind the same epitope, except for possible variant antibodies, e.g., containing naturally occurring mutations or arising during production of a monoclonal antibody preparation, such variants generally being present in minor amounts. In contrast to polyclonal antibody preparations, which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody of a monoclonal antibody preparation is directed against a single determinant on an antigen. Thus, the modifier “monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method. For example, the monoclonal antibodies to be used in accordance with the present invention may be made by a variety of techniques, including but not limited to the hybridoma method, recombinant DNA methods, phage-display methods, and methods utilizing transgenic animals containing all or part of the human immunoglobulin loci, such methods and other exemplary methods for making monoclonal antibodies being described herein.
In certain embodiments, the antibody is a multispecific antibody, e.g. a bispecific antibody. Multispecific antibodies are monoclonal antibodies that have binding specificities for at least two different sites. In certain embodiments, one of the binding specificities is for a first antigen and the other is for a different second antigen. In certain embodiments, multispecific antibodies may bind to two different epitopes of the same antigen. Multispecific antibodies may also be used to localize cytotoxic agents to cells which express the antigen. Multispecific antibodies can be prepared as full length antibodies or antibody fragments.
Techniques for making multispecific antibodies include, but are not limited to, recombinant co-expression of two immunoglobulin heavy chain-light chain pairs having different specificities (see Milstein, C. and Cuello, A. C., Nature 305 (1983) 537-540, WO 93/08829, and Traunecker, A., et al., EMBO J. 10 (1991) 3655-3659), and “knob-in-hole” engineering (see, e.g., U.S. Pat. No. 5,731,168). Multi-specific antibodies may also be made by engineering electrostatic steering effects for making antibody Fc-heterodimeric molecules (WO 2009/089004); cross-linking two or more antibodies or fragments (see, e.g., U.S. Pat. No. 4,676,980, and Brennan, M., et al., Science 229 (1985) 81-83); using leucine zippers to produce bi-specific antibodies (see, e.g., Kostelny, S. A., et al., J. Immunol. 148 (1992) 1547-1553; using “diabody” technology for making bispecific antibody fragments (see, e.g., Holliger, P., et al., Proc. Natl. Acad. Sci. USA 90 (1993) 6444-6448); and using single-chain Fv (sFv) dimers (see, e.g., Gruber, M., et al., J. Immunol. 152 (1994) 5368-5374); and preparing trispecific antibodies as described, e.g., in Tutt, A., et al., J. Immunol. 147 (1991) 60-69).
The antibody or fragment can also be a multispecific antibody as described in WO 2009/080251, WO 2009/080252, WO 2009/080253, WO 2009/080254, WO 2010/112193, WO 2010/115589, WO 2010/136172, WO 2010/145792, or WO 2010/145793.
Bispecific antibodies are generally antibody molecules that specifically bind to two different, non-overlapping epitopes on the same antigen or to two epitopes on different antigens.
Different bispecific antibody formats are known.
Exemplary bispecific antibody formats for which the methods as reported herein can be used are
In one embodiment the bivalent, bispecific antibody is a Crossmab.
In one embodiment the bivalent, bispecific antibody is a one-armed single chain antibody.
In one embodiment the bivalent, bispecific antibody is a two-armed single chain antibody.
In one embodiment the bivalent, bispecific antibody is a common light chain bispecific antibody.
The term “functional multimer” denotes a non-covalent complex or aggregate of multivalent, multispecific antibodies in which each of the multivalent, multispecific antibody molecules maintains its binding specificities, i.e. can still bind to its antigens. For example, a “functional dimer” is a non-covalent complex consisting of two molecules of the multivalent, multispecific antibody in which each of the binding sites maintains its binding specificity and binding ability, i.e. for example in total a functional dimer of a bivalent, bispecific antibody comprises four binding sites of which each two bind to the same antigen and all four binding sites are functional. But, if an antibody is present as functional multimer all antibody molecules can bind to immobilized antigen molecules (used to capture the multivalent, multispecific antibody) at the same time. This increases the complex stability of the functional multimer as compared to the monomeric molecule, and in turn the more unstable monomeric molecules might dissociate during the assay procedure. This results in that a significantly reduced amount of the monomeric molecule is present relative to the initial condition of the sample. As the detection of the complex is based on the interaction with a second binding specificity of the multivalent, multispecific antibody that it not used for the capturing of the antibody a captured immobilized functional dimer will result in an increased assay readout (see
For example a change of the fraction of functional multimers (dimers) in a tested antibody preparation results in different assay readouts if the more unstable complex interaction is immobilized. Thus, assays for determining the biological activity of multivalent, multispecific antibodies are sensitive to avidity-driven apparent increase of the biological activity.
An analytical procedure for measuring the biological activity of a test substance is based on a specific, functional biological response of a test system.
The term “biological assay” denotes an analytical method for determining the biological activity of a test substance, e.g. of an antibody, based on a specific, functional biological response of a test system. Biological activity may be assessed using animal based in-vivo assays, cell based in-vitro assays, or (receptor) binding assays. In one embodiment the term “biological activity” denotes the binding to the test substance to its interaction partner. In case of a bispecific antibody the term “biological activity” denotes the binding to the antibody to its respective antigens, either determined individually, i.e. separately for each antigen, or simultaneously, i.e. for both antigens concomitantly in a bridging assay.
Specificity is the ability to assess unequivocally the analyte in the presence of interfering components which may be expected to be present. Typically, these might include impurities, degradation products (such as modified analytes, fragments of the analyte, or aggregates of the analyte), matrix components (e.g. from serum), side-products (such as multimers of the analyte) etc.
Multivalent, multispecific antibodies specifically bind to different targets, most likely with different affinities and complex stabilities for each target. Only a fully active multivalent, multispecific antibody can bind to all targets and shows the full biological activity in a corresponding assay.
The term “valency” denotes the number of different binding sites of an antibody for an antigen. A monovalent antibody comprises one binding site for an antigen. A bivalent antibody comprises two binding sites for the same antigen.
An assay design involving first the binding of the multispecific molecule (antibody) to immobilized target #1 (its first antigen), and second the recruiting of target #2 (its second antigen) from solution to the target #1/multispecific molecule complex (antigen#1/multispecific antibody complex) covers both binding events with one activity readout (bridging assay, bridging binding signal).
Potential side-products of multispecific molecules are multimeric forms of the molecule, such as e.g. dimers, trimers, tetramers and higher order aggregates. If the multimeric form can still bind to both targets, the binding to each target is polyvalent rather than monovalent (=functional multimer).
The term “binding affinity” denotes the strength of the interaction of a single binding site with its respective target. Experimentally, the affinity can be determined e.g. by measuring the kinetic constants for association (kA) and dissociation (kD) of the antibody and the antigen in the equilibrium (see
The term “binding avidity” denotes the combined strength of the interaction of multiple binding sites of one molecule (antibody) with the same target. As such, avidity is the combined synergistic strength of bond affinities rather than the sum of bonds (see
The complex association does not differ between affine and avid binding. However, the complex dissociation for avid binding depends on the simultaneous dissociation of all binding sites involved (see
It has been found that functional multimers (e.g. dimers) of multivalent, multispecific antibodies (e.g. bivalent, bispecific antibodies) that are present in preparations containing such antibodies influence the result of an assay for the determination of the biological activity of the multivalent, multispecific antibody (see
It has been found that an assay (e.g. an SPR-based assay) for determining the biological activity of a multivalent, multispecific antibody (e.g. a bivalent, bispecific antibody) is not affected by the presence of functional multimers (dimers) of the multivalent, multispecific antibody if:
In more detail, like the monomeric multivalent, multispecific antibody, functional multimers (dimers) bind both targets with different complex stabilities. Functional multimers (dimers) are per definition multivalent (bivalent).
An assay for determining the biological activity of a multivalent, multispecific antibody using a bridging format can be set up using two different orientations: Either target #1 or target #2 can be immobilized on the surface and the second not immobilized target is recruited from solution to the target/multispecific molecule complex. Depending on the strength of the interaction and therewith on the stability of the formed complex the assay readout differs (see
It has been found that the assay setup has a strong impact on the specificity and robustness of an assay for determining the biological activity of a multivalent, multispecific antibody in the presence of functional multimers:
It has been found that low complex stability used for the capture of the antibody, i.e. on the surface, results in an assay signal that over-emphasizes functional multimers.
It has been found that high complex stability used for the capture of the antibody, i.e. on the surface, results in an assay signal that does not over-emphasizes functional multimers.
In addition to the readout with respect to the biological activity of the multivalent, multispecific antibody also the individual biological activity of the multivalent, multispecific antibody to the immobilized antigen can be determined.
Additionally to the readout with respect to the biological activity of the multivalent, multispecific antibody also the individual biological activity of the multivalent, multispecific antibody to the bridging antigen can be determined assuming that modifications affecting the binding to any of the antigens occur independently from each other and assuming that all antibodies bind to both antigens.
In
In
The use of SPR allows the determination of the biological activity in real-time.
The bridging assay as such and the use of the underlying findings as reported herein in an assay for determining the biological activity of a multivalent, multispecific antibody is based on the effect that by using the interaction with higher complex stability, i.e. smaller kD value (dissociation constant), for capture of the antibody the assay is less sensitive to functional multimers (dimers, trimers, tetramers, or oligomers). The total readout (=bridging binding signal;
In case of the use of SPR the sensograms harbor an additional readout besides the bridging signal: the binding signal to the immobilized antigen gives the opportunity to quantify molecules which are able to bind the immobilized first antigen (
The following examples and figures are provided to aid the understanding of the present invention, the true scope of which is set forth in the appended claims. It is understood that modifications can be made in the procedures set forth without departing from the spirit of the invention.
readout is based on this difference.
readout is based on this difference.
The method is based on an SPR-based assay using BIACORE® technology (GE Healthcare). In a first step, VEGF was immobilized to a CM5 sensor chip. Second, the anti-ANG2/VEGF bivalent, bispecific antibody was injected within a defined concentration range. Third, the human ANG2 (receptor binding domain RBD) was injected. The binding responses (resonance units, RU, see
In the first step, human ANG2 was immobilized on a micro titer plate (NUNC® Maxisorb-MTP). Second, the anti-ANG2/VEGF bivalent, bispecific antibody was added to the immobilized human ANG2 within a defined concentration range. Third, a constant amount of human VEGF was added. Fourth, a complex of mouse anti-VEGF antibody and goat anti-mouse IgG antibody-POD conjugate (POD=horseradish peroxidase) was added after pre-incubation. Finally, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid, ABTS) was added and after a defined time the reaction was stopped by the addition of phosphoric acid. The chromogenic ABTS signal was measured by an ELISA reader. The absorbance signals correlate with the amount of the bivalent, bispecific antibody bound to both human ANG2 and human VEGF and were plotted against the antibody concentration used. This sigmoidal plot was analyzed by XLfit4 (IDBS® Software), which fits a 4-parametric logistic curve and hence allows determination of the EC50 value as the biological activity readout.
Simultaneous binding of both antigens was confirmed by applying Surface Plasmon Resonance (SPR) using a BIACORE® T100 instrument (GE Healthcare Biosciences AB, Uppsala, Sweden). VEGF was immobilized to a CM5 Sensor chip using standard amine coupling chemistry. In a first step, the bivalent, bispecific antibody was injected at a concentration of 10 μg/ml in HBS buffer (10 mM HEPES, 150 mM NaCl, 0.05% TWEEN® 20 (polysorbate 20), pH 7.4) at 25° C. After binding of the antibody to the immobilized VEGF, human ANG2 was injected at 10 μg/ml in a second step.
In a further experiment the affinity and binding kinetics of the bivalent bispecific antibody were determined. Briefly, goat anti-huIgG-Fcgamma polyclonal antibodies were immobilized on a CM4 chip via amine coupling for presentation of the bispecific antibody against ANG2 and VEGF. Binding was measured in HBS buffer at 25° C. or 37° C. Purified ANG2-His or VEGF was added in various concentrations between 0.37 nM and 30 nM or between 3.7 nM and 200 nM in solution. Association was measured by an injection of 3 minutes; dissociation was measured by washing the chip surface with HBS buffer for 10 minutes and a KD value was estimated using a 1:1 Langmuir binding model. Due to heterogeneity of the ANG2 preparation no 1:1 binding could be observed. Therefore KD values are apparent values. The determined affinity of the bivalent, bispecific antibody to VEGF was extremely high, the calculated off-rate was out of BIACORE® specifications even at 37° C. In Table 1 the off-rates for both antigens are summarized.
Additionally to the SPR-based analysis, an ELISA was established to determine the binding of active bivalent, bispecific antibody. In this assay, human ANG2 is directly coated to the wells of a Maxisorb microtiter plate (MTP) in the first step. Meanwhile, the samples/reference standards (bivalent, bispecific antibody) were pre-incubated in the wells of another MTP with digoxigenylated VEGF. After pre-incubation and coating, excess of unbound ANG2 was removed by washing the ANG2 coated MTP. The pre-incubated mixture of bivalent, bispecific antibody and VEGF-Dig was then transferred to the human ANG2 coated MTP and incubated. After incubation, the excess of pre-incubation solution was removed by washing followed by incubation with a horse-radish peroxidase labeled anti-digoxigenin antibody. The antibody-enzyme conjugate catalyzes the color reaction of the ABTS® substrate. The signal was measured by ELISA reader at 405 nm wavelength (reference wavelength: 490 nm ([405/490] nm)).
| Number | Date | Country | Kind |
|---|---|---|---|
| 13197264.8 | Dec 2013 | EP | regional |
This application is a divisional of U.S. patent application Ser. No. 15/180,468, filed Jun. 13, 2016, which is a continuation of International Patent Application No. PCT/EP2014/076953, having an international filing date of Dec. 9, 2014, the entire contents of which are incorporated herein by reference, and which claims benefit under 35 U.S.C. § 119 to European Patent Application No. 13197264.8, filed on Dec. 13, 2013.
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15180468 | Jun 2016 | US |
| Child | 16998990 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/EP2014/076953 | Dec 2014 | US |
| Child | 15180468 | US |
