Stabilization of radiopharmaceutical compositions using hydrophilic 6-hydroxy-chromans

Abstract

Radiopharmaceutical compositions which are stabilized by addition of a hydrophilic 6-hydroxy-chroman derivative.

Description

BACKGROUND OF INVENTION

[0003] The present invention relates to novel stabilizers of radiopharmaceutical compositions used for diagnosis and therapy. In particular, the invention relates to use of a hydrophilic 6-hydroxy-chroman derivative to increase the shelf-life of diagnostic and therapeutic radiopharmaceuticals.

[0004] A number of radionuclides are routinely employed in nuclear medicine, both as diagnostic agents and as therapeutics. For example, 99mTc, 111In, 18F, and 201Tl are employed as diagnostic imaging agents, and 131I, 32P, 89Sr, and 153Sm are in therapeutic use. In addition, nuclides such as 186Re, 188Re, 212Bi, 213Bi, 90Y, 67Cu, 192Ir, 165Dy, and 117mSn have been proposed as potential therapeutic agents. Such radionuclides are administered in the form of radiopharmaceutical compositions, which generally include a chelator for the nuclide. Radiopharmaceuticals may additionally include a targeting molecule such as a monoclonal antibody, an antibody fragment, or a receptor ligand. The availability of radiopharmaceuticals has significantly advanced diagnosis and treatment of a variety of diseases.

[0005] Chemical decomposition may limit a radiopharmaceutical's shelf life by decreasing the radiochemical purity of the agent over time. For example, a radiopharmaceutical containing 99mTc, 186Re, or 188Re may be susceptible to oxidation of the nuclide itself. In addition, the radiation emitted from a radionuclide can break chemical bonds of other components of the composition, thus causing autoradiolysis. Autoradiolysis is a particular problem when the radiopharmaceutical contains higher energy nuclides, such as β-emitters (e.g., 186Re, 188Re, 90Y, 131I) and α-emitters (e.g., 213Bi, 212Bi, 211At, 225Ac, 223Ra).

[0006] Thus many radiopharmaceuticals require stabilizers to maximize shelf life. Such stabilizers must be non-toxic and must be able to maintain the product's radiochemical purity for an acceptable shelf-life as well as during use. In addition, an acceptable radiopharmaceutical stabilizer must not interfere with delivery of the radionuclide to the target site.

[0007] Methods for stabilizing radiopharmaceuticals by adding gentisates are disclosed, for example, in U.S. Pat. Nos. 4,232,000; 4,233,284; 4,497,744; 5,384,113. Stabilization of radiopharmaceuticals using ascorbic acid is disclosed in U.S. Pat. Nos. 5,393,512 and 5,011,676, in WO 97/28181 and in WO 98/33531. Hydroquinone stabilizers of radiopharmaceuticals is disclosed in U.S. Pat. No. 4,229,427. Other compounds such as reductic acid, erythorbic acid, ρ-aminobenzoic acid, 4-hydroxybenzoic acid, nicotinic acid, nicotinamide, 2,5-dihydroxy-1,4-benzenedisulfonic acid, tartaric acid, inositol, and the like, have also been used to stabilize radiopharmaceutical compositions.

[0008] U.S. Pat. No. 5,384,113 discloses a method of preventing autoradiolysis of peptides radiolabeled with 111In using gentisic acid or gentisyl alcohol. In addition to preventing autoradiolysis of peptides by 111In, the method of U.S. Pat. No. 5,384,113 is proposed to prevent autoradiolysis of peptides by 67Ga, 169Yb, 125I, 123I, and 201Tl. Two radiolabelled peptides, 111In-DTPA-octreotide and 123I-LHRH, were tested for autoradiolysis prevention. A monoclonal antibody, NR-Lu-10, labeled with 186Re was also specifically exemplified.

[0009] As indicated in Example 1, infra, the present inventors have found that that when added as a component in radiopharmaceutical kit formulations, gentisic acid decreases the radiochemical purity of some 99mTc-labelled peptides, and thus is not useful as a stabilizer of some radiolabeled peptides. A need exists, therefore, for additional stabilizers of radiopharmaceuticals. A particular need exists for stabilizers of radiopharmaceuticals containing less than 70 amino acids linked by peptide bonds.

[0010] U.S. Pat. Nos. 3,947,473; 4,003,919; 4,018,799 and 4,026,907 disclose a variety of antioxidant hydrophilic 6-hydroxy-chroman compounds as intermediates in preparation of optically active É-tocopherol. U.S. Pat. No.4,511,685 discloses hydrohilic 6-hydroxy-chroman derivatives and use of such derivatives to stabilize polypropylene compositions. U.S. Pat. Nos. 4,847,267 and 4,970,216 disclose use of one such hydrophilic 6-hydroxy-chroman hydrophilic 6-hydroxy-2,5,7,8-tetramethyl-2-carboxylic acid alone or in combination with sulfur compounds, including glutathione or cysteine, as a skin treatment composition to inhibit generation of free radicals in the skin.

SUMMARY OF THE INVENTION

[0011] It has now been surprisingly found that the radiolabelling efficiency and shelf-life of peptide and non-peptide radiopharmaceutical compositions may be significantly increased by addition of a stabilizing amount of a hydrophilic 6-hydroxy-chroman derivative.

[0012] In one embodiment, the invention provides a composition comprising a radiopharmaceutical precursor and a stabilizing amount of a hydrophilic 6-hydroxy-chroman derivative.

[0013] In another embodiment, the invention provides a method of stabilizing a radiopharmaceutical comprising the steps of:

[0014] a) combining a precursor of said radiopharmaceutical with a stabilizing amount of a hydrophilic 6-hydroxy-chroman dervative in a container; and

[0015] b) adding a radionuclide to the container.

[0016] In a further embodiment, the invention provides a kit comprising a sealed vial containing a predetermined quantity of a radiopharmaceutical precursor and a stabilizing amount of a hydrophilic 6-hydroxy-chroman derivative.

DETAILED DESCRIPTION OF THE INVENTION

[0017] The patent and scientific literature referenced herein establish the knowledge available to those with skill in the art. The issued U.S. patents are hereby incorporated by reference.

[0018] As defined herein, a “radiopharmaceutical” or “radiopharmaceutical composition” comprises a radionuclide, a chelator, and optionally a targeting moiety or domain.

[0019] In accordance with the invention, a “precursor” of a radiopharmaceutical is defined as comprising an unlabelled, that is, non-radioactive, reagent which may be a chelator or a chelator covalently linked to a targeting moiety or domain.

[0020] A “targeting moiety or domain” as defined herein as a moiety or domain capable of binding specifically to a site within a mammalian body such as a receptor on a cell surface. Targeting moieties or domains within the scope of the present invention include but are not limited to antibodies, antibody fragments such as Fab or F(ab)′2 fragments, epitope binding complementarity determining regions derived from antibodies, peptides, growth factors or receptor binding fragments thereof, hormones, steroids, receptor binding nucleic acids, receptor binding carbohydrates including monosaccharides, disaccharides, and oligosaccharides, receptor-binding lipids, benzodiazepines, receptor binding antibiotics, and the like.

[0021] A “stabilizing amount” is defined herein as that amount of hydrophilic 6-hydroxy-chroman sufficient to maintain the radiochemical purity, as measured by known methods such as those disclosed in the examples below, of a radiopharmaceutical composition relative to that of the radiopharmaceutical composition without the additive for at least 3 hours. Preferably, a clinically acceptable radiochemical purity for a radiopharmaceutical is at least 80% of the labelled undegraded radiopharmaceutical. More preferably, a clinically acceptable radiochemical purity for a radiopharmaceutical is at least 85% of the labelled undegraded radiopharmaceutical. Most preferably, a clinically acceptable radiochemical purity for a radiopharmaceutical is at least 90% of the labelled undegraded radiopharmaceutical.

[0022] A “hydrophilic 6-hydroxy-chroman derivative” is defined in accordance with the present invention as having a formula: 1

[0023] wherein

[0024] one of Y and Z is selected from the group consisting of O, S, C═O, and (CHR3)n where n is an interger from 0 to 3, and the other of Y and Z is selected from the group consisting of C═O and (CHR3)n where n is an integer from 0 to 3;

[0025] each R3 group is independently selected from the group consisting of H, alkyl, halogen, —OR4, —SO3H, —SO3R4, —S(O)mR4, —COOR4, —NO2, —CONHm(R4)2-m, —NHm(R4)2-m,—COR4, —CH2OR4, —COR5, —SO2NHm(R4)2-m, —R5, and —CH2R5, where m is an integer from 0 to 2;

[0026] R4 is H or C1 to C3 alkyl; and

[0027] R5 is selected from the group consisting of a monosaccharide, a disaccharide, and a hydrophilic peptide sequence of up to 5 amino acids comprising at least one hydrophilic amino acid residue.

[0028] Preferably, Y is (CH2) and Z is (CH2). Exemplary hydrophilic 6-hydroxy-chroman derivatives of the present invention include 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox®, available from Aldrich Chemcial Co., (Milwaukee, Wis., USA); 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid-4-sulfonic acid; 6-hydroxy-2,5,7,8-tetramethylchroman-3-hydroxy-2-carboxylic acid; 6-hydroxy-2,5,7,8-tetramethylchroman-2-glucosamine, having a structure: 2

[0029] and 6-hydroxy-2,5,7,8-tetramethylchroman-2-(carboxy-seryl-seryl-serylamide), having the structure: 3

[0030] Preferably, the hydrophilic 6-hydroxy-chroman derivative of the present invention is a water soluble vitamin E derivative. More preferably, the hydrophilic 6-hydroxy-chroman derivative of the invention is a 6-hydroxy-2,5,7,8-tetramethyl-2-carboxylic acid derivative having —CH2 at the 3- and 4- positions and a hydrophilic substituent at the 2-position. Most preferably, the hydrophilic 6-hydroxy-chroman derivative of the invention is 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.

[0031] Any radiopharmaceutical may be stabilized by addition of a hydrophilic 6-hydroxy-chroman as taught herein. Ligand-type radiopharmaceuticals which do not comprise a targeting moiety or domain, such as Tc 99m MAG3 (TechnoScan®, Mallinkrodt Medical, Inc., St. Louis, Mo., USA), may be stabilized in accordance with the present invention. In addition, radiopharmaceuticals comprising any kind of targeting moiety or domain may be stabilized in accordance with the present invention.

[0032] Recently a new class of radiopharmaceuticals has been developed which target a radiolabel to a particular tissue, disease site, or organ through a small receptor-specific molecule, which may be a peptide, a β-glucan, a benzodiazepine, or other small molecule.

[0033] Such radiopharmaceuticals are disclosed and claimed, for example, in commonly assigned U.S. Pat. Nos. 5,508,020; 5,225,180; 5,405,597; 5,443,815; 5,552,525; 5,561,220; 5,620,675; 5,645,815; 5,654,272; 5,681,541; 5,711,931; 5,714,579; 5,716,596; 5,736,122; 5,770,179; 5,783,170; 5,788,960; 5,807,537; 5,807,538; 5,811,394; 5,814,297; 5,814,298; 5,814,299; 5,820,845; 5,820,846; 5,830,856; 5,833,942; 5,843,401; 5,843,403; 5,849,260; 5,849,261; 5,851,509; 5,866,097; 5,871,711; 5,932,189; 5,951,964; 5,955,426; 5,976,496; 5,997,844; 6,007,792; 6,017,509; 6,017,512; 6,028,056; 6,051,206; 6,074,627; 6,086,850; 6,171,178 and; 6,241,960; and in commonly assigned copending U.S. patent application Ser. Nos. 08/236,402; 08/253,973; 08/721,443; and 09/553,494. These new agents comprise a chelator covalently linked to the receptor-specific targeting moiety or domain, and a radiolabel complexed with the chelator. A kit for making one such agent, ACUTECT®, has received approval in the U.S. for scintigraphic imaging of acute deep vein thrombosis. A second kit, NEOTECTOR®, has been approved in the U.S. for imaging malignant lung tumors. The stabilizers of the present invention are particularly suitable for use with radiopharmaceuticals which comprise chelators covalently linked to peptide, β-glucan, benzodiazepine, or other small targeting molecules as described in the commonly assigned patents and copending applications listed above.

[0034] In general, radiopharmaceuticals containing precursors in which a targeting moiety or domain is covalently linked to a monoamine, diamide, single thiol containing chelator such as those disclosed in commonly assigned copending U.S. patent application Ser. No. 08/253,973 and in WO 95/33497 are stabilized using a hydrophilic thioether, a hydrophilic 6-hydroxy-chroman or a mixture of a hydrophilic thioether and a hydrophilic 6-hydroxy-chroman in accordance with this invention. In addition, radiopharmaceuticals containing precursors in which a targeting moiety or domain is covalently linked to a bisamine bisthiol (BAT) chelator such as those disclosed in commonly assigned U.S. Pat. Nos. 5,780,007; 5,776,428; 5,720,934; 5,922,303; 5,965,107; 6,086,849; and 6,093,383 and in WO 93/21962 may be stabilized in accordance with the present invention.

[0035] The stabilizers of the present invention may also be used for radiopharmaceuticals comprising targeting molecules covalently linked to any chelator, such as the diamine monoamide thiol chelators and the triamine thiol chelators described in U.S. Pat. No. 5,688,485 and the triamide thiols disclosed in U.S. Pat. No. 5,091,514.

[0036] The stabilizers of the invention are preferably employed to increase the shelf life of radiopharmaceuticals comprising a targeting moiety covalently linked to a peptide metal chelator having a formula

C(pgp)s-(aa)-C(pgp)s

[0037] wherein (pgp)s is H or a thiol protecting group and (aa) is an amino acid. Such chelators are disclosed and claimed in commonly assigned U.S. Pat. Nos. 5,654,272; 5,681,541; 5,788,960; and 5,811,394.

[0038] The stabilizers of the invention may also be employed to increase the shelf life of radiopharmaceuticals comprising a targeting moiety covalently linked to a peptide metal chelator having a formula selected from the group consisting of: 4

[0039] wherein

[0040] X is H or a protecting group;

[0041] (amino acid) is any amino acid;

[0042] and 5

[0043] wherein

[0044] X is H or a protecting group;

[0045] (amino acid) is any amino acid.

[0046] Such chelators are disclosed and claimed in commonly assigned U.S. Pat. Nos. 5,720,934; 5,776,428; 5,780,007; 6,086,849 and 6,093,383.

[0047] More preferably, the stabilizers of the invention are used to increase the shelf life of radiopharmaceuticals comprising a targeting moiety covalently liked to a peptide metal chelator comprising a single thiol having a formula:

A-CZ(B)-[C(R′R″)]n,-X

[0048] wherein

[0049] A is H, HOOC, H2NOC, (peptide)-NHOC, (peptide)-OOC or R″″;

[0050] B is H, SH, —NHR′″, —N(R′″)-(peptide), or R″″;

[0051] X is H, SH, —NHR′″, —N(R′″)-(peptide) or R″″;

[0052] Z is H or R″″;

[0053] R′, R″, R′″ and R″″ are independently H or lower straight or branched chain or cyclic alkyl;

[0054] n is 0, 1 or 2;

[0055] and

[0056] where B is —NHR′″ or —N(R′″)-(peptide), X is SH, and n is 1 or 2;

[0057] where X is —NHR′″ or —N(R′″)-(peptide), B is SH, and n is 1 or 2;

[0058] where B is H or R″″, A is HOOC, H2NOC, (peptide)-NHOC, (peptide)-OOC, X is SH, and n is 0 or 1;

[0059] where A is H or R″″, then where B is SH, X is —NHR′″ or —N(R′″)-(peptide) and

[0060] where X is SH, B is —NHR′″ or —N(R′″)-(peptide);

[0061] where X is H or R″″, A is HOOC, H2NOC, (peptide)-NHOC, (peptide)-OOC and B is SH;

[0062] where Z is methyl, X is methyl, A is HOOC, H2NOC, (peptide)-NHOC, (peptide)-OOC, B is SH and n is 0.

[0063] Such chelators are disclosed and claimed in commonly assigned U.S. Pat. Nos. 5,443,815; 5,807,537; 5,814,297; and 5,866,097.

[0064] Specific embodiments of the single thiol containing radiometal chelator stabilized in accordance with the present invention are described and claimed in commonly assigned copending U.S. patent application Ser. No. 08/236,402 and in WO 95/29708, and include chelators having the chemical formula:

R1—CO-(amino acid)1-(amino acid)2-Z

[0065] wherein (amino acid)1 and (amino acid)2 are each independently any primary α- or β-amino acid that does not comprise a thiol group, Z is a thiol-containing moiety selected from the group consisting of cysteine, homocysteine, isocysteine, penicillamine, 2-mercaptoethylamine and 3-mercaptopropylamine, and R1 is lower (C1-C4) alkyl, an amino acid, or a peptide comprising 2 to 10 amino acids. When Z is cysteine, homocysteine, isocysteine or penicillamine, the carbonyl group of said moiety is covalently linked to a hydroxyl group, a NR3R4 group, wherein each of R3 and R4 are independently H or lower (C1-C4) alkyl, an amino acid or a peptide comprising 2 to 10 amino acids.

[0066] Alternatively, a single thiol containing radiometal chelator stabilized in accordance with the present invention has a formula:

Y-(amino acid)2-(amino acid)1-NHR2

[0067] wherein Y is a thiol-containing moiety that is cysteine, homocysteine, isocysteine, penicillamine, 2-mercaptoacetate or 3-mercaptopropionate, (amino acid)1 and (amino acid)2 are each independently any primary α- or β-amino acid that does not comprise a thiol group, and R2 is H or lower (C1-C4) alkyl, an amino acid or a peptide comprising 2 to 10 amino acids. When Y is cysteine, homocysteine, isocysteine or penicillamine, the amino group of said moiety is covalently linked to -H, an amino acid or a peptide comprising 2 to 10 amino acids.

[0068] Specific embodiments of the single thiol containing radiometal chelator are selected from the group consisting of:

[0069] -(amino acid)1-(amino acid)2-A-CZ(B)-{C(R1R2)}n-X},

[0070] -A-CZ(B)-{C(R1R2)}n-X}-(amino acid)1-(amino acid)2,

[0071] -(a primary α, ω- or β, ω-diamino acid)-(amino acid)1-A-CZ(B)-{C(R1R2)}n-X}, and

[0072] -A-CZ(B)-{C(R1R2)}n-X}-(amino acid)1-(a primary α, β- or α, ω-diamino acid) wherein the term “α, ω-diamino acid” represents an amino acid having an amine on the α carbon atom and an amine on the carbon atom most distal from the α carbon atom, the term “β, ω-diamino acid” represents an amino acid having an amine on the β carbon atom and an amine on the carbon atom most distal from the β carbon atom, and (amino acid)1 and (amino acid)2 are each independently any naturally-occurring, modified, substituted or altered α- or β-amino acid not containing a thiol group.

[0073] Specific single thiol-containing radiometal chelators stabilized in accordance with the invention have a formula selected from the group consisting of: -Gly-Gly-Cys-, Cys-Gly-Gly-, -(ε-Lys)-Gly-Cys-, (δ-Orn)-Gly-Cys-, -(γ-Dab)-Gly-Cys-, (β-Dap)-Lys-Cys-, and -(β-Dap)-Gly-Cys-. (In these formulae, ε-Lys represents a lysine residue in which the ε-amino group, rather than the typical α-amino group, is covalently linked to the carboxyl group of the adjacent amino acid to form a peptide bond; δ-Orn represents an ornithine residue in which the δ-amino group, rather than the typical α-amino group, is covalently linked to the carboxyl group of the adjacent amino acid to form a peptide bond; γ-Dab represents a 2,4-diaminobutyric acid residue in which the γ-amino group is covalently linked to the carboxyl group of the adjacent amino acid to form a peptide bond; and β-Dap represents a 2,3-diaminopropionic acid residue in which the β-amino group is covalently linked to the carboxyl group of the adjacent amino acid to form a peptide bond.)

[0074] Most preferably, the stabilizers of the invention may be used to increase the shelf life of radiopharmaceuticals comprising a targeting moiety covalently linked to a monoamine, diamide, single thiol metal chelator such as those disclosed and claimed in commonly assigned copending U.S. patent application Ser. No. 08/253,973 and in WO 95/33497, and to increase the shelf life of radiopharmaceuticals comprising a targeting moiety covalently linked to a bisamide bisthiol metal chelator such as those disclosed and claimed in commonly assigned U.S. Pat. Nos. 5,780,007; 5,922,303; 6,086,849; and 6,093,383. Exemplary monoamine, diamide, single thiol chelators stabilized by a mixture of a hydrophilic thioether and a hydrophilic 6-hydroxy chroman have general formulae selected from the group consisting of: 6

[0075] wherein n, m and p are each integers that are independently 0 or 1; each R′ is independently H, lower alkyl, C2-C4 hydroxyalkyl, or C2-C4 alkoxyalkyl, and each R is independently H or R″, where R″ is a substituted lower alkyl group, an unsubstituted lower alkyl group, or a phenyl not comprising a thiol group, and one R or R′ is L, where L is a bivalent linker linking the metal chelator to the targeting moiety and wherein when one R′ is L, NR′2 is an amine. In preferred embodiments, L is a C1-C6 linear alkyl group; a branched chain alkyl group; a cyclic alkyl group; a carboxylic ester; a carboxamide; a sulfonamide; an ether; a thioether; an amine; an alkene; an alkyne; a 1,2-linked, optionally substituted benzene ring; a 1,3-linked, optionally substituted benzene ring; a 1,4-linked, optionally substituted benzene ring; an amino acid, or a peptide of 2 to about 10 amino acids, or combinations thereof. In preferred embodiments, R″ is a C1-C6 linear alkyl group; a branched alkyl group; a cyclic alkyl group; a —CqOC1—, —CqNHC1— or —CqSCr— group, where q and r are integers each independently 1 to 5 wherein the sum of q+r is not greater than 6; a (C1-C6) alkyl-X, where X is a hydroxyl group; a substituted amine; a guanidine; an amidine; a substituted thiol group; a carboxylic acid; an ester; a phosphate group; a sulfate group; a phenyl group; a phenyl group substituted with a halogen, a hydroxyl, a substituted amine, a guanidine, an amidine, a substituted thiol, an ether, a phosphate group, or a sulfate group; an indole group; a C1-C6 heterocyclic group containing 1 to 3 nitrogen, oxygen or sulfur atoms; or a combination thereof.

[0076] In a specific embodiment, the monoamine, diamide single thiol radiometal chelator stabilized in accordance with the invention may have a formula: 7

[0077] wherein R1 and R2 are each independently H, lower alkyl, C2-C4 hydroxyalkyl, or C2-C4 alkoxyalkyl; R3, R4, R5 and R6 are independently H, substituted or unsubstituted lower alkyl or phenyl not comprising a thiol group; R7 and R8 are each independently H, lower alkyl, lower hydroxyalkyl or lower alkoxyalkyl; L is a bivalent linker group and Z is a targeting moiety.

[0078] The monoamine, diamide single thiol radiometal chelator stabilized in accordance with the invention may alternatively have a formula: 8

[0079] wherein R1 and R2 are each independently H, lower alkyl, C2-C4 hydroxyalkyl, or C2-C4 alkoxyalkyl; R3, R4, R5 and R6 are independently H, substituted lower alkyl, unsubstituted lower alkyl, phenyl, substituted phenyl not comprising a thiol group, and one of R3, R4, R5 or R6 is Z—L—HN(CH2)n—, where L is a bivalent linker, Z is a targeting moiety, and n is an integer from 1 to 6; R7 and R8 are each independently H, lower alkyl, lower hydroxyalkyl, lower alkoxyalkyl; and X is an amino group, a substituted amino group or —NR1—Y, where Y is an amino acid, an amino acid amide, or a peptide comprising from 2 to 10 amino acids.

[0080] The monoamine, diamide single thiol radiometal chelator stabilized in accordance with the invention may alternatively have a formula: 9

[0081] wherein R1 and R2 are each independently H, lower alkyl, lower hydroxyalkyl, or lower alkenylalkyl; R3 and R4 are independently H, substituted or unsubstituted lower alkyl or phenyl not comprising a thiol group; n is an integer from 1 to 6; L is a bivalent linker; and Z is a targeting moiety.

[0082] The monoamine, diamide single thiol radiometal chelator stabilized in accordance with the invention may alternatively have a formula: 10

[0083] wherein L is a bivalent linker and Z is a targeting moiety.

[0084] Bisamide bisthiol metal chelators stabilized in accordance with the present invention preferably have a formula selected from the group consisting of: 11

[0085] wherein

[0086] each R is independently H, CH3 or C2H5;

[0087] each (pgp)S is independently a thiol protecting group or H;

[0088] m, n and p are independently 2 or 3;

[0089] A is linear or cyclic lower alkyl, aryl, heterocyclyl, a combination thereof or a substituted derivative thereof;

[0090] and 12

[0091] wherein

[0092] each R is independently H, CH3 or C2H5;

[0093] m, n and p are independently 2 or 3;

[0094] A is linear or cyclic lower alkyl, aryl, heterocyclyl, a combination thereof or a substituted derivative thereof;

[0095] V is H or —CO-peptide;

[0096] R′ is H or peptide;

[0097] and wherein when V is H, R′ is peptide; and when R′ is H, V is —CO-peptide.

[0098] For example, the stabilizers of the invention may be used to increase the shelf life of radiopharmaceuticals comprising the specific precursors set forth below:

[0099] GGCSIPPEVKFNKPFVYLI.amide (SEQ ID NO:1);

[0100] GGCSIPPEVKFNKPFVYLI (SEQ ID NO:2);

[0101] GGCGLF (SEQ ID NO:3);

[0102] RGCSIPPEVKFNKPFVYLI.amide (SEQ ID NO:4);

[0103] RGCGHRPLDKKREEAPSLRPAPPPISGGYR.amide (SEQ ID NO:5);

[0104] GGCRPKPQQFFGLM.amide (SEQ ID NO:6);

[0105] GGCFVYLI.amide (SEQ ID NO:7);

[0106] (acetyl.TKPRGG)2K(ε-K)GC.amide;

[0107] FDFYWDKTFT(ε-K)GC.amide;

[0108] acetyl.FDFYWDKTFT(ε-K)GC.amide;

[0109] acetyl.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0110] acetyl.FDFYWDKTFTGGG(ε-K)GC.amide;

[0111] acetyl.FDFYWDKTFTGGG(ε-K)KC.amide;

[0112] acetyl.KKKKK.NalD.Cpa.YWDKTFT(ε-K)GC.amide;

[0113] acetyl.DDFD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0114] acetyl.DDFD.Cpa.YWDKTC(ε-K)GCK(K.amide;

[0115] acetyl.KKKKK.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0116] acetyl.NalD .Cpa.YWDKTFT(ε-K)GCKK.amide;

[0117] acetyl-DDD.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0118] acetyl.DDDFD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0119] (DTPA).FDFYWDKTFT(ε-K)GC.amide;

[0120] (DTPA).NalD.Cpa.YWDKT.Nal.T(ε-K)GCKK.amide;

[0121] (DTPA).(ε-K)GCFDFYWDKTFT.amide;

[0122] (DTPA).(ε-K)GCFD.Cpa..YWDKTFT.amide;

[0123] (DTPA).FD.Cpa.YWDKTFT(ε-K)GC.amide;

[0124] (DTPA).NalD.Cpa.YWDKTFT(ε-K)GC.amide;

[0125] (DTPA).Aca.FD.Cpa.YWDKTFT(ε-K)GC.amide;

[0126] (DTPA).NalD.Cpa.YWDKT.Nal.T(ε-K)GCKK.amide;

[0127] (DTPA).NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0128] CH2CO.FFWDKTFC (ε-K)GC.amide;

[0129] CH2CO.FFWDKTFC KKKKK(ε-K)GC.amide;

[0130] CH2CO.FFWDKTFC (ε-K)KKKKKGC.amide;

[0131] AKCGGGFDFYWDKTFT.amide;

[0132] AKCGGGFDYWDKTFT.amide;

[0133] DDDD.NalD.Cpa.YWDKTFT(ε-K)GCKKKK.amide;

[0134] DDD.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0135] NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0136] Trc.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0137] Hca.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0138] (Trc)2.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0139] KKKK.NalD.Cpa.YWDKTFT(ε-K)GCDDDD.amide;

[0140] KD.NalD.Cpa.YWDKTFT(ε-K)GCD.amide;

[0141] KDK.NalD.Cpa.YWDKTFT(ε-K)GCDD.amide;

[0142] KDKK.NalD.Cpa.YWDKTFT(ε-K)GCDDD.amide;

[0143] KDKK.NalD.Cpa.YWDKTFT(ε-K)GCDD.amide;

[0144] KDKKK.NalD.Cpa.YWDKTFT(ε-K)GCDD.amide;

[0145] KDKKK.NalD.Cpa.YWDKTFT(ε-K)GCKDKD.amide;

[0146] KDKKKFD.Cpa.YWDKTF,Nal.(ε-K)GCDDDD.amide;

[0147] K(BAT).NalD.CMcYWDKVCMcT.amide

[0148] KDDKD.NalD.Cpa.YWDKTFT(ε-K)GCKDKD.amide;

[0149] KDKD.NalD.Cpa.YWDKTFT(ε-K)GCKDKD.amide;

[0150] FD.Cpa.YWDKTC(ε-K)GCKK.amide;

[0151] FD.Cpa.YWDKTC(ε-K)GC.amide;

[0152] FD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0153] FD.Cpa.YWDK.Abu.Nal.T(ε-K)GC.amide;

[0154] FD.Cpa.YWDKTFTGGG(ε-K)GC.amide;

[0155] FD.Cpa.YWDKTFT(ε-K)GCR.amide;

[0156] (Trc-imide).NalD.Cpa.YWDKTFT(ε-K)GCR.amide;

[0157] Trc.(Trc-imide).K.NalD.Cpa.YWDKTFT(ε-K)GCRR.amide;

[0158] (Trc-imiide)2K.NalD.Cpa.YWDKTFT(ε-K)GCRR.amide;

[0159] (Trc-imide)2K.NalD.Cpa.YWDKTFT(ε-K)GCR.amide;

[0160] DDDFD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0161] DDFD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0162] FDFYWDKTFT(ε-K)GCKK.amide;

[0163] AKCGGGFDYWDKTFT.amide;

[0164] (2-ketogulonyl).NalD.Cpa.YWDKTFT(ε-K)GCKK.amide;

[0165] (2-ketogulonyl).FD.Cpa.YWDKTFT(ε-K)GC.amide;

[0166] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.GC.Dap.Dap.amide);

[0167] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.(γ-Dab)KCR.amide);

[0168] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.KKKKK(ε-K)GC.amide);

[0169] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO).(ε-K)GCK.amide;

[0170] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.(β-Dap)KCR.amide);

[0171] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.(β-Dap)KCK.amide);

[0172] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.(δ-Orn)GCK.amide);

[0173] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.(β-Dap)GCK.amide);

[0174] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.K(ε-K)KCK.amide);

[0175] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO.(ε-K)GCKK.amide);

[0176] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO).K(ε-K)GC.amide;

[0177] cyclo-(N-CH3)FYWDKV.Hcy(CH2CO).(ε-K)GC.amide;

[0178] RGCQAPLYKKIIKKLLES (SEQ ID NO:8);

[0179] acetyl.KK(ε-K)GCGCGGPLYKKIIKKLLES;

[0180] acetyl.KKKKKK(ε-K)GCGGPLYKKIIKKLLES;

[0181] (CH2CO.YDAmp.GDCKGCG.amide)2(CH2CO)2K(ε-K)GC.amide;

[0182] (CH2CO.YD.Amp.GDCGGCAcmGCAcmGGC.amide)2(CH2CO)2K(ε-K)GC.amide;

[0183] (CH2CO.YD.Apc.GDCKGCG.amide)2(CH2CO)2K(ε-K)GC.amide;

[0184] {(CH2CO.YD.Apc.GDCGGCG.amide)(CH2CO)}2K(ε-K)GC.amide;

[0185] (CH2CO.YD.Apc.GDCKGG)2K(ε-K)GC.β-Ala.amide;

[0186] (CH2CO.YD.Apc.GDCKKG)2K(ε-K)GC.β-Ala.amide;

[0187] {(CH2CO.YD.Apc.GDCG)2KG}2K(ε-K)GCG.amide;

[0188] (CH2CO.YD.Apc.GDC)2K(ε-K)GCG.amide;

[0189] ({(CH2CO.YD.Apc.GDCGGCAcmGCAcmGGC.amide)(CH2CO)}2.K)2K(ε-K)GCG.amide;

[0190] {(CH2CO.YD.Apc.GDCGGCAcmGCAcmGGC.amide)2(CH2CO)2K}2K(ε-K)GCG.amide;

[0191] (CH2CO.YD.Apc.GDCGGCAcmGCAcmGGC.amide)2(CH2CO)2K(ε-K)GC.amide;

[0192] HSDAVFTDNYTRLRKQMAVKKYLNSILN(ε-K)GC.amide;

[0193] HSDAVFTDNYTRLRKQMAVKKYLNSILNGGC.amide (SEQ ID NO:9);

[0194] AGCHSDAVFTDNYTRLRKQMAVKKYLNSILN.amide (SEQ ID NO:10);

[0195] HSDAVFTDNYTRLRKQMAVKKYLNSILNC(BAT).amide (SEQ ID NO:11);

[0196] CH2CO.SNLST.Hhc VLGKLSC(BAT)ELHKLQTYPRTNTGSGTP.amide (SEQ ID NO:12);

[0197] CH2CO.SNLST.Hhc VLGKLSQELHKLQTYPRTNTGSGTP(ε-K)GC.amide;

[0198] CH2CO.SNLST.Hhc VLGKLSC(CH2CO.GGCK.amide)ELHKLQTYPRTNTGSGTP.amide;

[0199] CH2CO.SNLST.Hhc VLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRTNTGSGTP.amide;

[0200] CH2CO.SNLST.Hhc VLGKLSC(CH2CO.(ε-K)GCE.amide)ELHKLQTYPRTNTGSGTP.amide;

[0201] CH2CO.SNLST.Hcy VLGKLSC(CH2CO.GGCK.amide)ELHKLQTYPRTNTGSGTP.amide;

[0202] CH2CO.SNLST.Hcy VLGKLSC(CH2CO.(β-Dap)KCK.amide)ELfIKLQTYPRTNTGSGTP.amide;

[0203] CH2CO.SNLST.Hcy VLGKLSC(CH2CO.(ε-K)GCE.amide)ELHKLQTYPRTNTGSGTP.amide;

[0204] CH2CO.SNLST.Cys. LGKLSC(CH2CO.GGCK.amide)ELHKLQTYPRTNTGSGTP.amide;

[0205] CH2CO.SNLST.Cys VLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRTNTGSGTP.amide;

[0206] CH2CO.SNLST.Cys VLGKLSC(CH2CO.(ε-K)GCE.amide)ELHKLQTYPRTNTGSGTP.amide;

[0207] SNLST.Asu VLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRINTGSGTP.amide;

[0208] SNLST.Asu VLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRTDVGAGTP.amide;

[0209] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Tyr-Cys-Thr(ol));

[0210] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Phe(4-F)-Cys-Thr(ol));

[0211] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Phe(4-NH2)-Cys-Thr-Ser);

[0212] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Dab-Cys-Thr);

[0213] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Phe(4-NH2)-Cys-Thr);

[0214] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Phe(4-NH2)-Cys-Thr(ol));

[0215] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-His-Cys-Thr(ol));

[0216] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Arg-Cys-Thr(ol));

[0217] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Gly-Cys-Lys-NH2);

[0218] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Ser-Cys-Thr(ol));

[0219] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Dab-Cys-Thr(ol));

[0220] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Gly-Cys-Thr(ol));

[0221] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Dab-Cys-Ser(ol));

[0222] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Gly-Cys-Lys-NH2);

[0223] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Gly-Cys-Arg-NH2);

[0224] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-Lys-NH2);

[0225] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-Arg-NH2);

[0226] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-Lys-Thr(ol));

[0227] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-Dap-NH2);

[0228] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-NH(CH2CH2O)2CH2CH2NH2);

[0229] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Ser-Cys-Thr-NH(CH2CH2O)2CH2CH2NH2);

[0230] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Lys-Cys-NH2);

[0231] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2Co-Ser-Lys-Cys-NH2);

[0232] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Lys-Gly-Cys-NH2);

[0233] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2Co-Ser-Dab-Cys-Ser(ol));

[0234] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2Co-Ser-Dap-Cys-NH2);

[0235] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Gly-Cys-His-NH2);

[0236] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Gly-Cys-Phe(4-NH2)-NH2);

[0237] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Orn-Cys-Thr(ol));

[0238] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Dap-Cys-Thr(ol));

[0239] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Lys-Cys-Thr(ol));

[0240] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-NHCH2CH2OCH2CH2NH2);

[0241] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Lys-Cys-NH2);

[0242] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-δ-Orn-Gly-Cys-NH2); and

[0243] cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Thr-Gly-Gly-Cys-NH2)

[0244] (Single-letter and three-letter abbreviations for amino acids can be found in G. Zubay, Biochemistry (2d. ed.), 1988 (MacMillan Publishing: New York) p.33; other abbreviations are as follows: Acm is acetamidomethyl; Mob is 4-methoxybenzyl; Abu is aminobutyric acid; FD is D-phenylalanine; WD is D-tryptophan; YD is D-tyrosine; Aca is 6-aminohexanoic acid; Ape is S-(3-aminopropyl)cysteine; Hcy is homocysteine; Nal is 2-naphthylalanine; Cpa is 4-chlorophenylalanine; KD is D-lysine; DD is D-aspartate; NalD is D-2-naphthylalanine; DTPA is diethylenetriaminepentaacetic acid; Trc is tricarballylic acid; Trc-imide is tricarballylic imide; and Hca is hexacarboxycyclohexane. ( . . . )2K represents covalent linkage to both amino groups of lysine. Hcy( . . . ) represents covalent linkage to the sidechain sulfur atom of homocysteine. (N—CH3)F represents N-α-methyl-phenylalanine. Underlining between groups (e.g., as between the CH2CO. group and cysteine (C) in CH2CO.YDRGDC) represents a cyclic sulfide. Underlining between amino acids (e.g., as between the cysteines (C) in CNPRGDC) represents a cyclic disulfide bond. The term “cyclo” before an underlined sequence means an N-terminus-to-C-terninus cyclic sequence. The subscript XD indicates the amino acid is in the D-configuration; all other subscripts refer to amino acid sidechain protecting groups. ε-K, δ-Orn, γ-Dab, and β-Dap are defined as set forth above. Asu is 2-amino suberic acid, wherein the amino terminal amino acids of peptides containing an Asu residue are cyclized via an amide bond between the amino terminal amino group and the side chain carboxylic acid moiety of the Asu residue. BAT is N6,N9-bis(2-mercapto-2-methylpropyl)-6,9-diazanonanoic acid.

[0245] In addition, a hydrophilic 6-hydroxy-chroman derivative may be used in accordance with the present invention to stabilize labelled radiopharmaceutical precursors comprising a benzodiazepine derivative, such as those described in U.S. Pat. No. 6,171,578. In a preferred embodiment, a hydrophilic 6-hydroxy-chroman derivative such as 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid is used to stabilize radiolabelled 1-[(carboxyglycyl-glycyl-glycyl-cysteinamide)methyl]-4-(2-carboxyethyl)-7-[(4-amidinophenyl)methyl]3,4-dihydro-1H-1,4-benzodiazepine-2,5-dione trifluoroacetate.

[0246] In addition, hydrophilic 6-hydroxy-chroman dervative may be used in accordance with the present invention to stabilize labeled radiopharmaceutical precursors comprising a targeting moiety or domain covalently linked to the known chelators 1,4,7,10-tetraazadodecanetetraacetic acid and derivatives thereof: 13

[0247] where n is an integer that is 2 or 3 and where each R is independently H, C1 to C4 alkyl, or aryl and one R is covalently linked to the targeting moiety, and desferrioxamine.

[0248] A radiopharmaceutical comprising any radionuclide or radiometal may be stabilized in accordance with the present invention. For example, radiopharmaceuticals containing such nuclides as 125I, 131I, 211At, 47Sc, 67Cu, 72Ga, 90Y, 153Sm, 159Gd, 165Dy, 166Ho, 175Yb, 177Lu, 186Re, 188Re, 212Bi, 213Bi, 68Ga, 99mTc, 111In, and 123I, and the like may be stabilized by addition of a hydrophilic 6-hydroxy chroman derivative in accordance with the invention. The extent of stabilization of a particular radiopharmaceutical precursor when chelated to different radionuclides may vary. For example, a 99Tc-labelled precursor may be stabilized to a greater extent than a 188Re-labelled form of the same precursor.

[0249] The compositions of the invention are formulated as a sterile, pyrogen-free, parenterally acceptable aqueous solution which may optionally be supplied in lyophilized form and be reconstituted by the user. The compositions of the invention may be provided as components of kits which may include buffers, additional vials, instructions for use, and the like.

[0250] The pharmaceutical compositions of the invention comprises a radiopharmaceutical precursor in combination with a stabilizing amount of a hydrophilic 6-hydroxy-chroman, optionally with a pharmaceutically acceptable diluent or a carrier such as species appropriate albumin. As used herein, a “pharmaceutically acceptable diluent or carrier” may include any and all solvents, dispersion media, antibacterial and antifungal agents, isotonic agents, enzyme inhibitors, transfer ligands such as glucoheptonate, tartrate, citrate, or mannitol, and the like. The use of such media and agents for pharmaceutically active substances is well known in the art. For example, Sodium Chloride Injection and Ringer's Injection are commonly used as diluents. The preparation of such parenterally acceptable solutions, having due regard to pH, isotonicity, stability, and the like, is within the skill in the art.

[0251] In accordance with the method of this invention, radiopharmaceuticals are preferably administered intravenously in a single unit dose, either totally as a bolus or partly as a bolus followed by infusion over 1-2 hours. The amount of solution to be injected at unit dosage is from about 0.01 mL to about 10 mL, containing about 0.01 mCi to about 100 mCi of radioactivity, preferably from about 1 mCi to about 50 mCi. The amount of the radiopharmaceutical in the unit dose may range from about 0.1 to about 10 mg/kg body weight, After intravenous administration, the site is monitored, for example, by radioimaging in vivo if the radiopharmaceutical is a diagnostic agent.

[0252] The following examples are shown by way of illustration and not be considered as limitations.

EXAMPLE 1

Effect of Gentisic Acid on Radiochemical Purity of 99mTc-labelled Depreotide

[0253] Gentisic acid (GA) was tested for its ability to stabilize the 99mTc-labelled somatostatin receptor-binding peptide depreotide, which has the structure. 14

[0254] This peptide is represented as:

cyclo(N—CH3)FYWDKV.Hcy.(CH2CO.(β-Dap)KCK.amide)

[0255] in the listing set forth above.

[0256] Lyophilized kit vials were prepared containing depreotide, GA, and other components as described in Table 1. Formulations were adjusted to pH 7.4 or 8.5 (as noted) prior to lyophilization.

TABLE 1
Component	Control	GA I	GA II	GA III
Depreotide	50 μg	50 μg	50 μg	50 μg
Sodium Glucoheptonate	25 mg	25 mg	5 mg	25 mg
Dihydrate1
Edetate Disodium	100 μg	100 μg	100 μg	100 μg
Dihydrate2
Stannous Chloride	50 μg	50 μg	50 μg	50 μg
Dihydrate3
Gentisic Acid Sodium	—	1 mg	1 mg	1 mg
Salt Hydrate4
pH	7.4	7.4	7.4	8.5
1Pfanstiehl Laboratories, Waukegan, Illinois, USA.
2J. T. Baker, Phillipsburg, New Jersey, USA.
3Acros Organics/Fisher Scientific, Pittsburgh, Pennsylvania, USA.
4Sigma Chemical Co., St. Louis, Missouri, USA.

[0257] The lyophilized kits were radiolabelled with 99mTc by reconstitution with 1.0 mL technetium 99Tc sodium pertechnetate (Technelite® Molybdenum Mo99-Technetium Tc99m Generator, DuPont, Billerica, Mass.) containing approximately 50 mCi 99mTc and heating in a boiling water bath for 10 minutes. Radiolabelling yield (RCP) results as measured by reversed phase HPLC are given in Table 2.

	TABLE 2
	HPLC RCP (%)
	Formulation	0.5 hr	3.5 hr	6.5 hr
	Control	94.5	88.3	86.4
		94.2	92.1	90.8
		94.5	91.7	90.1
	(Average ± 1SD):	(94.4 ± 0.2)	(90.7 ± 2.1)	(89.1 ± 2.4)
	GA I	82.4	79.4	77.2
	GA II	29.1	25.1	20.5
	GA III	0.9	0.7	0.6

[0258] These results indicate that gentisic acid decreases the radiolabelling yield and the stability of 99mTc-depreotide when included in formulated kits.

EXAMPLE 2

Stabilization of 99mTc-labelled Depreotide by Trolox®

[0259] Lyophilized kit vials were prepared containing depreotide, Trolox®, and other components as described in Table 3. All formulations were adjusted to pH 7.4 prior to lyophilization.

	TABLE 3
	Trolox	Trolox	Trolox	Trolox
Component	Control	I	II	III	IV
Depreotide	50 μg	50 μg	50 μg	50 μg	50 μg
Sodium	5 mg	5 mg	5 mg	5 mg	5 mg
Glucoheptonate
Dihydrate
Edetate Disodium	100 μg	100 μg	100 μg	100 μg	100 μg
Dihydrate
Stannous	50 μg	50 μg	50 μg	50 μg	50 μg
Chloride
Dihydrate
Trolox	—	0.6 mg	1 mg	2 mg	5 mg

[0260] The lyophilized kits were radiolabelled with 99mTc by reconstitution with 1.0 mL technetium 99mTc sodium pertechnetate (Technelite®) containing approximately 50 mCi 99mTc and incubation at room temperature for 30 minutes following reconstitution. Some of the formulations were also radiolabelled in a heated preparation (heat in a boiling water bath for 10 minutes). Radiolabelling yield (RCP) results as measured by reversed phase HPLC are given in Table 4.

	TABLE 4
	HPLC RCP (%)
Formulation	Prep Type	0.5 hr	3.5 hr	6.5 hr
Control	Heated	92.0	85.9	84.5
	Heated	91.4	85.3	78.3
(Average):		(91.7)	(85.6)	(81.5)
	Rm Temp	92.0	85.0	84.2
	Rm Temp	92.6	85.2	80.7
	Rm Temp	92.0	81.4	79.5
	Rm Temp	89.5	82.8	—
(Average ± 1SD):		(91.5 ± 1.4)	(83.6 ± 1.8)	(81.5 ± 2.4)
Trolox I (600 μg)	Rm Temp	94.3	93.2	92.0
	Rm Temp	91.8	88.6	89.1
(Average):		(93.1)	(90.9)	(90.6)
Trolox II (1 mg)	Rm Temp	91.3	89.6	91.0
	Rm Temp	92.9	91.8	92.5
	Rm Temp	94.1	93.2	91.1
(Average ± 1SD):		(92.8 ± 1.4)	(91.5 ± 1.8)	(91.5 ± 0.8)
Trolox III (2 mg)	Heated	94.9	91.1	85.6
	Heated	95.3	92.9	88.7
(Average):		(95.1)	(92.0)	(87.2)
	Rm Temp	95.4	94.8	95.4
	Rm Temp	94.5	93.7	93.8
	Rm Temp	95.5	—	92.2
	Rm Temp	93.8	91.7	92.4
	Rm Temp	94.8	92.4	93.0
	Rm Temp	—	94.6	93.5
(Average ± 1SD):		(94.8 ± 0.7)	(93.4 ± 1.4)	(93.4 ± 1.2)
Trolox IV (5 mg)	Rm Temp	93.3	92.0	—
	Rm Temp	92.1	94.8	93.8
(Average):		(92.7)	(93.4)	(93.8)

[0261] These results indicate that Trolox® increases the radiolabelling yield and the stability of 99mTc depreotide prepared from formulated kits.

EXAMPLE 3

[0262] Stabilization of 99mTc Depreotide by Trolox® in Lyophilized Kit Preparations; Accelerated Temperature (40° C.) Storage

[0263] Lyophilized kits were prepared containing depreotide, Trolox®, and other components as described in Table 5. All formulations were adjusted to pH 7.4 prior to lyophilization. The kits were stored for one week at 40° C. Some kits were also stored at −10° C. as controls.

TABLE 5
Component	Control	Trolox
Depreotide	50 μg	50 μg
Sodium Glucoheptonate	5 mg	5 mg
Dihydrate
Edetate Disodium	100 μg	100 μg
Dihydrate
Stannous Chloride	50 μg	50 μg
Dihydrate
Trolox ®	—	2 mg

[0264] The lyophilized kits were radiolabelled with 99mTc by reconstitution with 1.0 mL technetium 99mTc sodium pertechnetate (Technelite®) containing approximately 50 mCi 99mTc and incubation either at room temperature (30 minutes) or in a boiling water bath (10 min). Radiolabelling yield (RCP) results as measured by reversed phase HPLC are given in Table 6.

	TABLE 6
	HPLC RCP (%)
Formulation	Storage Temp	Prep Type	0.5 hr	3.5 hr	6.5 hr
Control	−10° C.	Heated	—	82.6	77.8
	40° C.	Heated	—	82.6	79.0
Trolox ®	−10° C.	Rm Temp	94.4	92.9	92.3
	40° C.	Rm Temp	86.6	89.2	88.6

[0265] These results indicate that the Trolox® stabilizes 99mTc-depreotide prepared from lyophilized kits which had been thermally stressed under conditions of accelerated temperature storage.

EXAMPLE 4

Stabilization of a 99mTc-labelled Peptide by Trolox

[0266] Trolox® was tested for its ability to stabilize a 99mTc-labelled glycoprotein IIb/IIIa receptor-binding peptide having the structure. 15

[0267] This peptide is represented as:

(CH3CO.YD.Amp.GDC.KGCG.amide)2(CH2CO)2K(ε-K)GC.amide

[0268] in the listing set forth above.

[0269] Lyophilized kit vials were prepared containing the peptide (50 μg), sodium glucoheptonate dihydrate (10 mg), stannous chloride dihydrate (50 μg), and edetate disodium dihydrate (100 μg). The formulation was adjusted to pH 7.4 prior to lyophilization.

[0270] The lyophilized kits were radiolabelled with 99mTc in the presence and absence of Trolox®. To the Trolox® preparation was added 2 mg Trolox® in 100 μL ethanol and 100 μL saline. The ethanol was necessary to aid in the dissolution of the Trolox®. To the control preparation was added 100 μL ethanol and 100 μL saline to account for the additional saline or ethanol added with the Trolox. Both vials were then reconstituted with 1.0 mL technetium 99mTc sodium pertechnetate (Technelite®) containing approximately 50 mCi 99mTc and allowed to incubate for 30 minutes at room temperature. Radiolabelling yield (RCP) results as measured by reversed phase HPLC are given in Table 7.

	TABLE 7
	HPLC RCP (%)
	Preparation	0.5 hr	3.5 hr	6.5 hr
	Control	91.8	80.4	76.2
	Trolox ® (2 mg)	89.5	91.9	92.9

[0271] These results show that Trolox® increases the radiolabelling yield and the stability of 99mTc-peptide.

EXAMPLE 5

Stabilization of 99mTc-labelled Peptide Chelator by Trolox®

[0272] Trolox® was tested for its ability to stabilize a 99mTc-labelled monoamine, diamide, single thiol peptide chelator having the structure. 16

[0273] N-3-benzoyl-2,3-(S)-diaminopropionyl-L-lysinyl-L-cysteinyl-L-lysinyl amide

[0274] Lyophilized kit “placebo” vials were prepared containing sodium glucoheptonate dihydrate, edetate disodium dihydrate, and stannous chloride dihydrate at the concentrations set forth in Table 1 (control formulation).

[0275] The peptide chelator was radiolabelled with 99mTc in the presence and absence of Trolox® The peptide chelator was dissolved in water at a concentration of 1 mg/mL, and 50 μg (50 μL) of the peptide was added to each of three placebo vials. Ethanol and Trolox® were added to the control and Trolox®, preparations as described in Example 11. In addition, 100 μL phosphate buffered saline (PBS) was added to each preparation. The vials were reconstituted with 0.9-1.0 mL 99mTc sodium pertechnetate (Technelite®) containing approximately 50 mCi 99mTc, and heated in a boiling water bath for ten minutes. Radiolabelling yield (RCP) results as measured by reversed phase HPLC are given in Table 8.

	TABLE 8
	HPLC RCP (%)
	Preparation	0.5 hr	3 hr	6 hr	9 hr
	Control	94.1	92.4	85.9	80.0
	Trolox ® (2 mg)	95.3	95.4	91.5	86.4

[0276] These results show that Trolox® increases the radiolabelling yield and the stability of a 99mTc-labeled peptide chelator.

EXAMPLE 6

Stabilization of a 99mTc Bisamide Bisthiol Chelator by Trolox®

[0277] Trolox® was tested for its ability to stabilize a 99mTc-labelled non-peptide chelator (4-(butanoic acid)-2,2,9,9 tetramethyl-4,7-diaza-1,10-decanedithiol) having the structure. 17

[0278] The non-peptide chelator was radiolabelled with 99mTc in the presence and absence of Trolox® using the placebo vial heated preparation procedure as described in Example 4. Radiolabelling yield (RCP) results as measured by reversed phase HPLC are given in Table 9.

	TABLE 9
	HPLC RCP (%)
	Preparation	0.5 hr	3 hr	6 hr	9 hr
	Control	48.5	56.5	54.0	52.9
	Trolox ® (2 mg)	88.6	79.1	78.3	77.0

[0279] These results show that Trolox® increases the radiolabelling yield and the stability of a 99mTc-labelled non-peptide chelator.

[0280] It should be understood that the foregoing disclosure emphasizes certain specific embodiments of the invention and that all modifications or equivalents thereto are within the spirit and scope of the invention as set forth in the appended claims.

Claims

1. A composition comprising a radiopharmaceutical precursor and a stabilizing amount of a hydrophilic 6-hydroxy-chroman derivative.

2. The composition of claim 1 wherein the 6-hydroxy-chroman derivative has the formula:

3. The composition of claim 2 wherein, in the formula, both X and Y are —CH2—.

4. The composition of claim 2, wherein the hydrophilic 6-hydroxy-chroman is selected from the group consisting of 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid-4-sulfonic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-3-hydroxy-2-carboxylic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-glucosamine and 6-hydroxy-2,5,7,8-tetramethylchroman-2-(carboxy-seryl-seryl-serylamide).

5. The composition of claim 4, wherein the hydrophilic 6-hydroxy-chroman is 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid.

6. The composition of claim 2, wherein the precursor comprises a targeting moiety selected from the group consisting of an antibody, a Fab antibody fragment, a F(ab)′2 antibody fragment, an epitope binding complementarity determining region derived from an antibody, a peptide, a growth factor, a receptor binding fragment of a growth factor, a hormone, a steroid, a receptor binding nucleic acid, a receptor binding monosaccharide, a receptor binding disaccharide, a receptor binding oligosaccharide, a receptor-binding lipid, a receptor binding benzodiazepine derivative, and a receptor binding antibiotic.

7. The composition of claim 6, wherein the targeting moiety is a peptide.

8. The composition of claim 6, wherein the targeting moiety is a glycoprotein IIb/IIIa receptor-binding benzodiazepine derivative.

9. The composition of claim 2, wherein the precursor comprises a peptide chelator.

10. The composition of claims 2, wherein the precursor comprises a non-peptide chelator.

11. The composition of claims 1 or 2, further comprising a radionuclide.

12. The composition of claim 11, wherein the radionuclide is selected from the group consisting of 125I, 131I, 211At, 47Sc, 67Cu, 72Ga, 90Y, 153Sm, 159Gd, 165Dy, 166Ho, 175Yb, 177Lu, 212Bi, 213Bi, 68Ga, 99mTc, 111In, and 123I.

13. A composition comprising a stabilizing amount of a 6-hydroxy-chroman derivative according to claim 2 and a peptide selected from the group consisting of: GGCSIPPEVKFNKPFVYLI.amide (SEQ ID NO:1); GGCSIPPEVKFNKPFVYLI (SEQ ID NO:2); GGCGLF (SEQ ID NO:3); RGCSIPPEVKFNKPFVYLI.amide (SEQ ID NO:4); RGCGHRPLDKKREEAPSLRPAPPPISGGYR.amide (SEQ ID NO:5); GGCRPKPQQFFGLM.amide(SEQ ID NO:6); GGCFVYLI.amide (SEQ ID NO:7); (acetyl.TKPRGG)2K(ε-K)GC.amide; FDFYWDKTFT(ε-K)GC.amide; acetyl.FDFYWDKTFT(ε-K)GC.amide; acetyl.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; acetyl.FDFYWDKTFTGGG(ε-K)GC.amide; acetyl.FDFYWDKTFTGGG(ε-K)KC.amide; acetyl.KKKKK.NalD.Cpa.YWDKTFT(ε-K)GC.amide; acetyl.DDFD.Cpa.YWDKTFT(ε-K)GCKK.amide; acetyl.DDFD.Cpa.YWDKTC(ε-K)GCKK.amide; acetyl.KKKKK.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; acetyl.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; acetyl-DDD.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; acetyl.DDDFD.Cpa.YWDKTFT(ε-K)GCKK.amide; (DTPA).FDFYWDKTFT(ε-K)GC.amide; (DTPA).NalD.Cpa..YWDKT.Nal.T(ε-K)GCKK.amide; (DTPA).(ε-K)GCFDFYWDKTFT.amide; (DTPA).(ε-K)GCFD.Cpa..YWDKTFT.amide; (DTPA).FD.Cpa.YWDKTFT(ε-K)GC.amide; (DTPA).NalD.Cpa.YWDKTFT(ε-K)GC.amide; (DTPA).Aca.FD.Cpa. YWDKTFT(ε-K)GC.amide; (DTPA).NalD.Cpa.YWDKT.Nal.T(ε-K)GCKK.amide; (DTPA).NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; CH2CO.FFWDKTFC(ε-K)GC.amide; CH2CO.FFWDKTFCKKKKK(ε-K)GC.amide; CH2CO.FFWDKTFC(ε-K)KKKKKGC.amide; AKCGGGFDFYWDKTFT.amide; AKCGGGFDYWDKTFT.amide; DDDD.NalD.Cpa.YWDKTFT(ε-K)GCKKKK.amide; DDD.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; Trc.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; Hca.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; (TrC)2.NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; KKKK.NalD.Cpa.YWDKTFT(ε-K)GCDDDD.amide; KD.NalD.Cpa.YWDKTFT(ε-K)GCD.amide; KDK.NalD.Cpa.YWDKTFT(ε-K)GCDD.amide; KDKK.NalD.Cpa.YWDKTFT(ε-K)GCDDD.amide; KDKK.NalD.Cpa.YWDKTFT(ε-K)GCDD.amide; KDKKK.NalD.Cpa.YWDKTFT(ε-K)GCDD.amide; KDKKK.NalD.Cpa.YWDKTFT(ε-K)GCKDKD.amide; KDKKKFD.Cpa.YWDKTF,Nal.(ε-K)GCDDDD.amide; K(BAT).NalD.CMeYWDKVCMcT.amide KDDKD.NalD.Cpa.YWDKTFT(ε-K)GCKDKD.amide; KDKD.NalD.Cpa.YWDKTFT(ε-K)GCKDKD.amide; FD.Cpa.YWDKTC(ε-K)GCKK.amide; FD.Cpa.YWDKTC(ε-K)GC.amide; FD.Cpa.YWDKTFT(ε-K)GCKK.amide; FD.Cpa.YWDK.Abu.Nal.T(ε-K)GC.amide; FD.Cpa.YWDKTFTGGG(ε-K)GC.amide; FD.Cpa.YWDKTFT(ε-K)GCR.amide; (Trc-imide).NalD.Cpa.YWDKTFT(ε-K)GCR.amide; Trc.(Trc-imide).K.NalD.Cpa.YWDKTFT(ε-K)GCRR.amide; (Trc-imide)2K.NalD.Cpa.YWDKTFT(ε-K)GCRR.amide; (Trc-imide)2K.NalD.Cpa.YWDKTFT(ε-K)GCR.amide; DDDFD.Cpa.YWDKTFT(ε-K)GCKK.amide; DDFD.Cpa.YWDKTFT(ε-K)GCKK.amide; FDFYWDKTFT(ε-K)GCKK.amide; AKCGGGFDYWDKTFT.amide; (2-ketogulonyl).NalD.Cpa.YWDKTFT(ε-K)GCKK.amide; (2-ketogulonyl).FD.Cpa.YWDKTFT(ε-K)GC.amide; cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.GC.Dap.Dap.amide); cyclo.(N—CH3)FYWDKV.Hcy(CH2CO.(γ-Dab)KCR.amide); cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.KKKKK(ε-K)GC.amide); cyclo-(N—CH3)FYWDKV.Hcy(CH2CO).(ε-K)GCK.amide; cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.(β-Dap)KCR.amide); cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.(β-Dap)KCK.amide); cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.(δ-Orn)GCK.amide); cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.(β-Dap)GCK.amide); cyclo-(N—CH3)FYWDKV.Hcy(CH2CO K(ε-K)KCK.amide); cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.(ε-K)GCKK.amide); cyclo-(N—CH3)FYWDKV.Hcy(CH2CO).K(ε-K)GC.amide; cyclo-(N—CH3)FYWDKV.Hcy(CCH2CO).(ε-K)GC.amide; RGCQAPLYKKIIKKLLES (SEQ ID NO:8); acetyl.KK(ε-K)GCGCGGPLYKKIIKKLLES; acetyl.KKKKKK(ε-K)GCGGPLYKKIIKKLLES; (CH2CO.YD.Amp.GDC.KGCG.amide)2(CH2CO)2K(ε-K)GC.amide; (CH2CO.YD.Amp.GDC.GGCAcmGCAcmGGC.amide)2(CH2CO)2K(ε-K)GC.amide; (CH2CO.YD.Apc.GDCKGCG.amide)2(CH2CO)2K(ε-K)GC.amide; {(CH2CO.YD.Apc.GDCGGCG.amide)(CH2CO)}2K(ε-K)GC.amide; (CH2CO.YD.Apc.GDCKGG)2K(ε-K)GC.β-Ala.amide; (CH2CO.YD.Apc.GDCKKG)2K(ε-K)GC.β-Ala.amide; {(CH2CO.YD.Apc.GDCG)2KG}2.K(ε-K)GCG.amide; (CH2 CO.YD.Apc.GDC)2K.(ε-K)GCG.amide; ({(CH2CO.YD.Apc.GDCGGCAcmGCAcmGGC.amide)(CH2CO)}2K)2K(ε-K)GCG.amide; {(CH2CO.YD.Apc.GDCGGCAcmGCAcmGGC.amide)2(CH2CO)2K}2K(ε-K)GCG.amide; (CH2CO.YD.Apc.GDCGGCAcmGCAcmGGC.amide)2(CH2CO)2K(ε-K)GC.amide; HSDAVFTDNYTRLRKQMAVKKYLNSILN(ε-K)GC.amide; HSDAVFTDNYTRLRKQMAVKKYLNSILNGGC.amide (SEQ ID NO:9); AGCHSDAVFTDNYTRLRKQMAVKKYLNSILN.amide (SEQ ID NO:10); HSDAVFTDNYTRLRKQMAVKKYLNSILNC(BAT).amide (SEQ ID NO:11); CH2CO.SNLST.HhcVLGKLSC(BAT)ELHKLQTYPRTNTGSGTP.amide (SEQ ID NO:12); CH2CO.SNLST.HhcVLGKLSQELHKLQTYPRTNTGSGTP(ε-K)GC.amide; CH2CO.SNLST.HhcVLGKLSC(CH2CO.GGCK.amide)ELHKLQTYPRTNTGSGTP.amide; CH2CO.SNLST.HhcVLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRTNTGSGTP.amide; CH2CO.SNLST.HhcVLGKLSC(CH2CO.(ε-K)GCE.amide)ELHKLQTYPRTNTGSGTP.amide; CH2CO.SNLST.HcyVLGKLSC(CH2CO.GGCK.amide)ELHKLQTYPRTNTGSGTP.amide; CH2CO.SNLST.HcyVLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRTNTGSGTP.amide; CH2CO.SNLST.HcyVLGKLSC(CH2CO.(ε-K)GCE.amide)ELHKLQTYPRTNTGSGTP.amide; CH2CO.SNLST.CysVLGKLSC(CH2CO.GGCK.amide)ELHKLQTYPRTNTGSGTP.amide; CH2CO.SNLST.CysVLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRTNTGSGTP.amide; CH2CO.SNLST.CysVLGKLSC(CH2CO.(ε-K)GCE.amide)ELHKLQTYPRTNTGSGTP.amide; SNLST.AsuVLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRTNTGSGTP.amide; SNLST.AsuVLGKLSC(CH2CO.(β-Dap)KCK.amide)ELHKLQTYPRTDVGAGTP.amide; cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Tyr-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Phe(4-F)-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Phe(4-NH2)-Cys-Thr-Ser); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Dab-Cys-Thr); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Phe(4-NH2)-Cys-Thr); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Phe(4-NH2)-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-His-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Arg-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Gly-Cys-Lys-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Ser-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Dab-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Gly-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Dab-Cys-Ser(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Gly-Cys-Lys-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Gly-Cys-Arg-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-Lys-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-Arg-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-Lys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-Dap-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-NH(CH2CH2O)2CH2CH2NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Ser-Cys-Thr-NH(CH2CH2O)2CH2CH2NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Lys-Cys-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Lys-Cys-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Lys-Gly-Cys-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Dab-Cys-Ser(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Dap-Cys-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Gly-Cys-His-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Gly-Gly-Cys-Phe(4-NH2)-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Orn-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Dap-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Lys-Cys-Thr(ol)); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Ser-Ser-Cys-NHCH2CH2OCH2CH2NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-β-Dap-Lys-Cys-NH2); cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-δ-Orn-Gly-Cys-NH2); and cyclo-Tyr-D-Trp-Lys-Thr-Phe-(N—CH3)Hcy(CH2CO-Thr-Gly-Gly-Cys-NH2).

14. The composition of claim 13, wherein the stablizer is 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.

15. The composition of claim 14, wherein the peptide is cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.(β-Dap)KCK.amide).

16. The composition of any of claims 13 through 15, further comprising a radionuclide.

17. The composition of claim 16, wherein the radionuclide is 99mTc.

18. A composition comprising a hydrophilic 6-hydroxy-chroman derivative 1-[carboxyglycly-glycyl-glycyl-cysteinamide)methyl]-4-(2 carboxyethyl)-7-[(4-amidinophenyl)methyl]-3,4-dihydro-1H-1,4-benzodiazepine-2,5-dione trifluoroacetate.

19. The composition of claim 18, wherein the hydrophilic 6-hydroxy-chroman derivative is 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.

20. The composition of claims 18 or 19, further comprising 99mTc.

21. A method of stabilizing a radiopharmaceutical comprising the steps of: a) combining a precursor of said radiopharmaceutical with a stabilizing amount of a hydrophilic 6-hydroxy-chroman derivative in a container; and b) adding a radionuclide to the container.

22. The method of claim 21 wherein the hydrophilic 6-hydroxy-chroman derivative is 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.

23. The method of any of claims 21 or 22, wherein the radionuclide is 99mTc.

24. A kit comprising a sealed vial containing a predetermined quantity of a radiopharmaceutical precursor and a stabilizing amount of a hydrophilic 6-hydroxy-chroman derivative.

25. The kit of claim 24, wherein the hydrophilic 6-hydroxy-chroman derivative is 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.

26. The kit of claim 25, wherein the precursor is cyclo-(N—CH3)FYWDKV.Hcy(CH2CO.(β-Dap)KCK.amide).

27. The kit of claim 25, wherein the precursor is: 1-[(carboxyglycyl-glycyl-glycyl-cysteinamide)methyl]-4-(2-carboxyethyl)-7-[(4-amidinophenyl)methyl]-3,4-dihydro-1H-1,4-benzodiazepine-2,5-dione trifluoroacetate.