Claims
- 1. A stabilized aqueous enzyme composition consisting essentially of;
- (a) from 0% to about 75% of a detergent surfactant selected from the group consisting of nonionic, anionic, cationic, zwitterionic, amphoteric and semipolar nonionic surfactants and mixtures thereof;
- (b) from about 0.025 to about 10% pure enzyme selected from the group consisting of proteolytic and amylolytic enzymes and mixtures thereof;
- (c) from 0 to about 60% of a low molecular weight primary or secondary alcohol selected from the group consisting of methanol, ethanol, propanol, isopropanol, polyols containing from 2 to about 6 carbon atoms and from 2 to about 6 hydroxy groups and mixtures thereof;
- (d) from about 0.1% to about 10% of a short chain length carboxylic acid salt selected from the group consisting of formates, acetates, propionates and mixtures thereof;
- (e) a soluble calcium salt to give from about 0.1 to about 10 millimoles of calcium ion per liter; and
- (f) the balance water,
- the pH of the product being from about 6.5 to about 10, the amount of calcium ion per liter being less than about 2 millimoles at pH's below about 8.5, and at pH's above 8.5, (d) is a formate.
- 2. The composition of claim 1 containing from about 5% to about 15% of said alcohol; from about 0.3% to about 3% of said carboxylic acid salt; and from about 0.1 to about 2 millimoles of calcium ion per liter, the pH being from about 7.5 to about 8.5 and said enzyme being a proteolytic enzyme.
- 3. The composition of claim 2 wherein the alcohol is selected from the group consisting of methanol, ethanol, propanol, ethylene glycol, propylene glycol, glycerine and isopropanol, and mixtures thereof.
- 4. The composition of claim 3 wherein the carboxylic acid salt is a formate.
- 5. The composition of claim 3 wherein the surfactant is present in an amount of from about 20% to about 50%.
- 6. The composition of claim 3 wherein the detergent surfactant is a mixture of anionic and nonionic surfactants.
- 7. The composition of claim 3 wherein the detergent surfactant is a mixture of nonionic and cationic surfactants.
- 8. The composition of claim 3 wherein the detergent surfactant is a mixture of nonionic and semi-polar nonionic surfactants.
- 9. The composition of claim 3 wherein the alcohol is ethyl alcohol and the carboxylic acid salt is a formate.
- 10. The composition of claim 4 wherein the detergent surfactant is present at a level of from about 20% to about 40%.
- 11. The composition of claim 10 wherein the formate is present at a level of from about 0.5% to about 1.5%.
- 12. The composition of claim 3 wherein the proteolytic enzyme is present at a level of from about 0.05% to about 0.2% to give a level of enzyme activity of from about 15 to about 60 Anson units per liter, wherein the enzyme has an isoelectric point of at least about 8.5, and wherein the carboxylic acid salt is a formate.
- 13. The composition of claim 12 wherein the surfactant is present at a level of from about 20% to about 50.
- 14. The composition of claim 13 wherein the alcohol is ethyl alcohol.
- 15. A stabilized aqueous enzyme composition consisting essentially of;
- (a) from 0% to about 75% of a detergent surfactant selected from the group consisting of nonionic, anionic, cationic, zwitterionic, amphoteric and semipolar nonionic surfactants and mixtures thereof;
- (b) from about 0.025 to about 10% pure enzyme selected from the group consisting of proteolytic and amyolytic enzymes and mixtures thereof;
- (c) from 0 to about 60% of a low molecular weight primary or secondary alcohol selected from the group consisting of methanol, ethanol, propanol, isopropanol, polyols containing from 2 to about 6 carbon atoms and from 2 to about 6 hydroxy groups and mixtures thereof;
- (d) from about 0.1% to about 10% of a formate;
- (e) a soluble calcium salt to give from about 0.1 to about 10 millimoles of calcium ion per liter; and
- (f) the balance water, the pH of the product being from about 6.5 to about 10.
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of our copending applications Ser. No. 092,906, filed Nov. 9, 1979, and Ser. No. 123,857, filed Feb. 22, 1980, both for STABILIZED AQUEOUS ENZYME COMPOSITION and both now abandoned.
US Referenced Citations (6)
Related Publications (1)
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Number |
Date |
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123857 |
Feb 1980 |
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Continuation in Parts (1)
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Number |
Date |
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92906 |
Nov 1977 |
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