Claims
- 1. A water soluble protein polymer conjugate comprising a protein and an acrylic polymer having a main chain and a molecular weight of at least about 5,000, wherein three or more linker groups bond the protein to the acrylic polymer, the linker groups being covalently bonded to the protein and to the acrylic polymer, said linker groups having at least about three hydroxyl groups and not more than 60 carbon atoms, each of the linker groups being selected from the group consisting of ##STR25## wherein E=--OH or --SH;
- n=0 or 1;
- W=0 or 1; and
- Y=bond or --CH.sub.2 ;
- wherein A is bonded to the polymer and is selected from the group consisting of ##STR26## B is bonded to the protein and is selected from the group consisting of ##STR27## wherein R.sub.1 and R.sub.2 are each selected from the group consisting of H, OH, acetoxy, alkoxy, phenylene, straight chain alkyl or branched chain alkyl having 1 to 12 carbons, ##STR28## wherein x=2 to 7, ##STR29## 2-hydroxy ethyl, 3-chloro-2-hydroxypropyl, 2-hydroxy- 1-methylethyl, 2-hydroxypropyl, 3-hydroxypropyl, 2, 3-dihydroxypropyl, 2 hydroxybutyl, 4-hydroxybutyl, diethylene-glycol, 5-hydroxypentryl, 6-hydroxyhexyl, triethyleneglycol, 7-hydroxyheptyl, 3, 4-dihydroxybutyl, 5-hydroxypentyl, 6-hydroxyhexyl, 2, 3-dimethyl-3- hydroxybutyl, 5,6-dihydroxyhexyl, 7-hydroxyheptyl, a residue of a mono-, di-, and trisaccharide;
- wherein R.sub.3 selected from the group consisting of H, straight chain alkyl or branched chain alkyl having 1 to 12 carbons, ##STR30## wherein x=2 to 7, ##STR31## 2-hydroxy ethyl, 3-chloro-2-hydroxypropyl, 2-hydroxy-1-methylethyl, 2-hydroxypropyl, 3-hydroxypropyl, 2, 3-dihydroxypropyl, 2 hydroxybutyl, 4-hydroxybutyl, diethylene-glycol, 5-hydroxypentryl, 6-hydroxyhexyl, triethyleneglycol, 7-hydroxyheptyl, 3, 4-dihydroxybutyl, 5-hydroxypentyl, 6-hydroxyhexyl, 2, 3-dimethyl-3-hydroxybutyl, 5,6-dihydroxyhexyl, 7-hydroxyheptyl, a residue of a mono-, di-, and trisaccharide; and
- R'.sub.1,R'.sub.2, R'.sub.3, R'.sub.4, R'.sub.5, R'.sub.6, R'.sub.7, R'.sub.8, and R'.sub.9 each selected from the group consisting of H, OH, acetoxy, alkoxy, halogen and phosphate, straight chain alkyl or branched chain alkyl having 1 to 12 carbons, ##STR32## wherein x=2 to 7, ##STR33## 2-hydroxy ethyl, 3-chloro-2-hydroxypropyl, 2-hydroxy-1-methylethyl, 2-hydroxypropyl, 3-hydroxypropyl, 2, 3-dihydroxypropyl, 2 hydroxybutyl, 4-hydroxybutyl, diethylene-glycol, 5-hydroxypentryl, 6-hydroxyhexyl, triethyleneglycol, 7-hydroxyheptyl, 3, 4-dihydroxybutyl, 5-hydroxypentyl, 6-hydroxyhexyl, 2, 3-dimethyl-3-hydroxybutyl, 5,6-dihydroxyhexyl, 7-hydroxyheptyl, residue of a mono-, di-, and trisaccharide.
- 2. The water soluble protein polymer conjugate as recited in claim 1 wherein R'.sub.1, R'.sub.2, R'.sub.3, R'.sub.4, R'.sub.5, R'.sub.6, R'.sub.7, R'.sub.8, and R'.sub.9 are selected from the group consisting of H, OH and an alkyl having from 1 to 4 carbon atoms wherein each of the linker groups is a residue of a saccharide selected from the group consisting of a monosaccharide, disaccharide and trisaccharide.
- 3. The protein polymer conjugate as recited in claim 2 wherein the protein is bonded to each of the linker groups through the residue of a lysine group.
- 4. The protein polymer conjugate as recited in claim 1 wherein the R'.sub.1 through R'.sub.9 are H or OH and the bond between the protein and each of the linker groups is a residue of a lysine group on the protein.
- 5. The protein polymer conjugate as recited in claim 1 wherein the R'.sub.1 through R'.sub.9 are H or OH and the bond between the protein and each of the linker groups is a residue of an arginine group on the protein.
- 6. The protein polymer conjugate as recited in claims 4 or 5 wherein each of the linker groups is ##STR34##
- 7. The protein polymer conjugate as recited in claims 4 or 5 wherein each of the linker groups is ##STR35##
- 8. The protein polymer conjugate as recited in claims 4 or 5 wherein each of the linker groups is ##STR36##
- 9. The protein polymer conjugate as recited in claims 4 or 5 wherein each of the linker groups is ##STR37##
- 10. The protein polymer conjugate as recited in claims 4 or 5 wherein each of the linker groups is ##STR38##
- 11. The protein polymer conjugate as recited in claims 4 or 5 wherein the linker group is ##STR39##
- 12. The protein polymer conjugate as recited in claims 4 or 5 wherein each of the linker groups is ##STR40##
- 13. The protein polymer conjugate as recited in claims 4 or 5 wherein each of the linker groups is ##STR41##
- 14. The protein polymer conjugate as recited in claim 1, wherein each of the linker groups is a residue of the monosaccharide selected from the group consisting of 6-amino-6-deoxy-D-glucose, 2-amino-2-deoxy-D-glucose, 3-amino-3-deoxy-D-glucose and mixtures thereof.
- 15. The protein polymer conjugate as recited in claim 1, wherein each of the linker groups is a residue of 6-amino-6-deoxy-D-glucose.
Parent Case Info
This Application is a Continuation-In-Part application of Ser. No. 613,224, filed November 14, 1990 now abandoned.
Government Interests
The research related to this application was supplied under several arrangements one of which was contract DE-AC03-76SF00098 between the U.S. Department of Energy and the University of California for the operation of Lawrence Berkeley Laboratory. In relation to the contract, the U.S. Government has certain interests in the invention.
US Referenced Citations (10)
Foreign Referenced Citations (5)
Number |
Date |
Country |
161188 |
Nov 1985 |
EPX |
450628 |
Nov 1991 |
EPX |
2019502 |
Jul 1970 |
FRX |
2600897 |
Jan 1988 |
FRX |
57-202309 |
Dec 1982 |
JPX |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
613224 |
Nov 1990 |
|