Claims
- 1. A substantially dried, purified allergenic extract substantially free from extracted components with molecular weights outside the range of 1000 to 100,000 daltons, having an allergen composition substantially the same as the original extract, and which upon aqueous reconstitution yields a transparent, colorless solution which has an increase in absorbance in the range of 400 to 700 nm of less than 0.01 O.D. after storage for 18 days at 22.degree. C.
- 2. An insoluble support to which a purified allergenic extract of claim 1 is adhered.
- 3. An insoluble support to which a conjugate of the purified allergenic extract of claim 1 and a water-soluble protein is adhered.
- 4. The purified stabilized allergenic extract of claim 1 wherein the extract is obtained from a member selected from the group consisting of pollens, epidermals, glandular elements, molds, smuts, insects, insect venoms and foods.
- 5. The purified allergenic extract of claim 4 obtained from a pollen.
- 6. The purified allergenic extract of claim 4 obtained from an epidermal or glandular element of an animal.
- 7. The purified allergenic extract of claim 4 obtained from a mold.
- 8. The purified allergenic extract of claim 4 obtained from a smut.
- 9. The purified allergenic extract of claim 4 obtained from an insect.
- 10. The purified allergenic extract of claim 4 obtained from an insect venom.
- 11. The purified allergenic extract of claim 4 obtained from a food.
- 12. A process for increasing stability of an allergenic extract comprising
- (a) passing an aqueous solution of an allergenic extract through 100,000 and 1000 dalton ultrafilters and retaining substantially all of the fraction having a molecular weight of from 1000 to 100,000 to yield an allergenic extract;
- (b) contacting the allergenic extract obtained in Step (a) with a quantity of a carbon absorbent and with a gel polymer absorbent which forms a cage structure in aqueous media which is sufficient to clarify the extract, to yield a retained fraction; and
- (c) substantially drying the retained fraction obtained in Step (b) to yield an allergenic product which upon aqueous reconstitution yields a transparent, colorless solution which has an increase in absorbance in the range of 400 to 700 nm of less than 0.01 O.D. after storage for 18 days at 22.degree. C.
- 13. A purified allergenic extract consisting essentially of the product of claim 12.
- 14. The process of claim 12 wherein the carbon absorbent has an average particle size of less than 200 mesh.
- 15. A purified allergenic extract consisting essentially of the product of claim 14.
- 16. The process of claim 12 wherein the allergenic extract is a fat-free, aqueous extract of a member selected from the group consisting of pollens, epidermals, glandular element, molds, smuts, insects, insect venoms and foods.
- 17. A purified allergenic extract consisting essentially of the product of claim 16.
- 18. The process of claim 12 wherein the swelled polymer is selected from the group consisting of a polysaccharide, polyacrylamide, polyvinylpyrrolidone and mixtures thereof.
- 19. A purified allergenic extract consisting essentially of the product of claim 18.
- 20. The process of claim 18 wherein the swelled polymer is a polysaccharide derived from dextran or agarose.
- 21. A purified allergenic extract consisting essentially of the product of claim 20.
- 22. The process of claim 20 wherein the carbon absorbent is activated charcoal having a particle size of less than 200 mesh and the swelled polymer is derived from dextran.
- 23. A purified allergenic extract consisting essentially of the product of claim 22.
Parent Case Info
This application is a Continuation of copending application Ser. No. 476,187 filed Mar. 17, 1983 abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3983053 |
Courtney et al. |
Sep 1976 |
|
4350686 |
Relyveld et al. |
Sep 1982 |
|
4387091 |
Vijay et al. |
Jun 1983 |
|
Non-Patent Literature Citations (1)
Entry |
Curtis, (1975), Allergenic Extracts Chapter 74, pp. 1344-1352, in: Remington's Pharmaceutical Sciences 15th Edition, Mack Publ. Co. |
Continuations (1)
|
Number |
Date |
Country |
Parent |
476187 |
Mar 1983 |
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