Patent Application
20100119489
This invention provides a novel protein involved in pancreatic β-cell proliferation and/or insulin secretion, nucleic acids and constructs encoding the same, and cells comprising the same. The invention also provides diagnostic and therapeutic applications of the TMEM27 protein.
Pancreatic β-cell hyperplasia is an important adaptive mechanism to maintain normoglycemia during physiological growth and in obesity. Increasing evidence suggests that the β-cell mass is dynamic and that increased demands on insulin secretion in insulin resistance and pregnancy can lead to rapid and marked changes in the β-cell mass. The mass of β-cells is governed by the balance of β-cell growth (replication) and by β-cell death (apoptosis), however, the molecular basis of the factors that control β-cell mass remain elusive. Understanding how β-cell mass is regulated is important to design rational approaches to prevent pancreatic β-cell loss in insulin resistant states and to expand β-cells for transplantation in type 1 diabetes.
During development, β-cells are generated from a population of pancreatic progenitor cells. The β-cells that differentiate from progenitor cells are postmitotic, and direct lineage tracing studies indicate that a population of progenitor cells persists throughout embryogenesis to allow the differentiation of new β-cells. In the neonatal period new β-cells are formed by replication of differentiated β-cells, which results in a massive increase in β-cell mass. In adulthood there is little increase in the β-cell number except in conditions of increased demand. During pregnancy a marked hyperplasia of the β-cells is observed both in rodents and man. This is due to increased mitotic activity of β-cells exposed to placental lactogen (PL), prolactin (PRL) and growth hormone (GH). The growth stimulatory signals in pathological insulin resistant states are less well understood.
Several mouse models of insulin resistance and diabetes, such as the ob/ob and db/db mice or mutant mice created by inactivation of the gene for insulin receptor substrate-1 (IRS-1) or double heterozygous (DH) knockout of the insulin receptor and IRS-1, exhibit marked islet hyperplasia. In contrast, loss of IRS2 function leads to a dramatic reduction of β-cells and diabetes. Glucose itself is known to stimulate β-cell replication, however, many of the above mouse models increase their total islet mass before the onset of detectable hyperglycemia. Furthermore, in most cases the hyperplastic response bears no relationship to the level of hyperglycemia, suggesting that factors independent of glucose likely contribute to the islet growth.
Endocrine pancreatic growth during development depends on multiple transcription factors that display highly specific temporal and spatial expression patterns that control mechanisms for cell differentiation. During early pancreatic development, cell fate decisions and differentiation of endocrine progenitor cells into hormone-producing islet cells is tightly regulated by the sequential expression of specific transcription factors. The expression of these factors is also important for maintaining normal pancreatic β-cell function in adult life. For instance, mutations in several transcription factors lead to a subtype of type 2 diabetes called maturity-onset diabetes of the young (MODY), which are characterized by autosomal dominant inheritance, an early age of disease-onset, and development of marked to hyperglycemia with a progressive impairment in insulin secretion. The most frequent form of MODY is caused by mutations in the gene encoding hepatocyte nuclear factor (Tcf1). Mutant mice with loss of Tcf1 function as well as transgenic mice expressing a naturally occurring dominant-negative form of human HNF-1 (P291fsinsC) in pancreatic β-cells exhibit progressive hyperglycemia with age due to impaired glucose-stimulated insulin secretion. These mice exhibit a progressive reduction in β-cell number, proliferation rate, and pancreatic insulin content. These data suggest that Tcf-1 target genes are also required for maintenance of normal β-cell mass, though their identity is as yet unknown.
In one embodiment, this invention provides an isolated nucleic acid comprising a regulatory region of a gene encoding a TMEM27 protein, wherein said regulatory region comprises at least one high-affinity binding site for a Tcf1 protein. In one embodiment, the regulatory region has a nucleic acid sequence corresponding to or homologous to SEQ ID NO: 10 or 11. In another embodiment, the binding site comprises a nucleic acid sequence corresponding to or homologous to SEQ ID NO: 12, 13, 14, or 15. In another embodiment, this invention provides a cell, or in another embodiment, a vector comprising a nucleic acid of this invention.
In another embodiment, this invention provides an isolated polypeptide, comprising a secreted form of a TMEM27 protein, wherein said polypeptide is about 25 kDa in size, and comprises an N-terminal fragment of a sequence corresponding to, or homologous to, SEQ ID NO: 16.
In another embodiment, this invention provides a method of increasing pancreatic β-cell mass, said method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a TMEM27 polypeptide and providing conditions favorable for β-cell proliferation.
In another embodiment, this invention provides a method of increasing pancreatic β-cell mass, said method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a nucleic acid encoding a TMEM27 polypeptide and providing conditions favorable for expression of said nucleic acid.
In another embodiment, this invention provides a method of altering metabolism in a subject, said method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a TMEM27 polypeptide or a nucleic acid encoding said TMEM27 polypeptide.
In another embodiment, this invention provides a method of inhibiting, suppressing or treating diabetes in a subject, said method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a TMEM27 polypeptide or a nucleic acid encoding said TMEM27 polypeptide.
In another embodiment, this invention provides a method of assessing pancreatic islet mass in a subject, the method comprising the step of determining a concentration of TMEM27 in the serum of said subject and comparing said concentration versus that of a control.
In another embodiment, this invention provides a method for identifying proteins which interact with a TMEM27 protein, or a secreted form thereof, the method comprising the step of contacting a TMEM27 polypeptide with a molecule of interest for a time and under conditions sufficient to facilitate specific interaction between said polypeptide and said molecule of interest and identifying said molecule of interest.
In another embodiment, this invention provides a method of increasing pancreatic β-cell mass, said method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β- with a serine or metallo protease inhibitor and providing conditions favorable for expression of said nucleic acid.
In another embodiment, this invention provides a method of altering metabolism in a subject, said method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a serine or metallo protease inhibitor.
In another embodiment, this invention provides a method of inhibiting, suppressing or treating diabetes in a subject, said method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a serine or metallo protease inhibitor.
In the following detailed description, numerous specific details are set forth in order to provide a thorough understanding of the invention. However, it will be understood by those skilled in the art that the present invention may be practiced without these specific details. In other instances, well-known methods, procedures, and components have not been described in detail so as not to obscure the present invention.
This invention provides, in one embodiment, a pancreatic, β-cell specific protein, which participates in β-cell proliferation, and insulin secretion.
As described herein, a frequent form of MODY is caused by mutations in the gene encoding hepatocyte nuclear factor (Tcf1). Mutant mice with loss of Tcf1 function in pancreatic β-cells exhibit progressive hyperglycemia with age due to impaired glucose-stimulated insulin secretion. The mice exhibit a progressive reduction in β-cell number, proliferation rate, and pancreatic insulin content, indicating that Tcf-1 target genes are also required for maintenance of normal β-cell mass. Tcf1 is a direct activator of TMEM27 transcription, as demonstrated herein. TMEM27 is shown herein to be expressed during pancreas development in hormone positive cells and restricted to pancreatic β-cells in the mature pancreas. TMEM27 is shown to be a glycoprotein, specifically cleaved and released by β-cells in the pancreas, and is a stimulator of β-cell replication in vitro and in vivo.
In one embodiment, this invention provides an isolated nucleic acid comprising a regulatory region of a gene encoding a TMEM27 protein, wherein said regulatory region comprises at least one high-affinity binding site for a Tcf1 protein.
In one embodiment, the term “regulatory region” refers to a promoter, which is a DNA sequence, which, in one embodiment, is upstream of a coding sequence, important for basal and/or regulated transcription of a gene.
In one embodiment, the promoter is an endogenous promoter, comprising at least one high-affinity binding site for a Tcf1 protein. In another embodiment, the promoter is a mutant of the endogenous promoter, which comprises at least one high-affinity binding site for a Tcf1 protein. Mutations in the promoter sequence may enhance Tcf1 binding, or in another embodiment, delay Tcf1 disengagement, or, in another embodiment, via any means, alter Tcf1 binding to the TMEM27 promoter, and thereby influence its transcription. In another embodiment, such mutants will be evaluated for their promoter strength, in terms of the resulting levels of expression of TMEM27.
In one embodiment, the regulatory region has a nucleic acid sequence corresponding to or homologous to SEQ ID NO: 10 or 11.
The nucleic acids used in this invention can be produced by any synthetic or recombinant process such as is well known in the art. Nucleic acids can further be modified to alter biophysical or biological properties by means of techniques known in the art. For example, the nucleic acid can be modified to increase its stability against nucleases (e.g., “end-capping”), or to modify its lipophilicity, solubility, or binding affinity to complementary sequences. These nucleic acids may comprise the vector, the expression cassette, the promoter sequence, the gene of interest, or any combination thereof.
DNA according to the invention can also be chemically synthesized by methods known in the art. For example, the DNA can be synthesized chemically from the four nucleotides in whole or in part by methods known in the art. Such methods include those described in Caruthers (1985). DNA can also be synthesized by preparing overlapping double-stranded oligonucleotides, filling in the gaps, and ligating the ends together (see, generally, Sambrook et al. (1989) and Glover et al. (1995)). DNA expressing functional homologues of the protein can be prepared from wild-type DNA by site-directed mutagenesis (see, for example, Zoller et al. (1982); Zoller (1983); and Zoller (1984); McPherson (1991)). The DNA obtained can be amplified by methods known in the art. One suitable method is the polymerase chain reaction (PCR) method described in Saiki et al. (1988), Mullis et al., U.S. Pat. No. 4,683,195, and Sambrook et al. (1989).
In another embodiment, the Tcf1 binding site comprises a nucleic acid sequence corresponding to or homologous to SEQ ID NO: 12, 13, 14, 15.
In another embodiment, this invention provides a cell, or in another embodiment, a vector comprising a nucleic acid of this invention.
In one embodiment, the term “vector” refers to a nucleic acid construct containing a sequence of interest that has been subcloned within the vector.
To generate the vectors of the present invention, polynucleotides encoding the TMEM27 regulatory region, and in some embodiment, the coding region for TMEM27, and, in some embodiments, other sequences of interest can be ligated into commercially available expression vector systems suitable for transducing/transforming eukaryotic or prokaryotic cells and for directing the expression of recombinant products within the transduced/transformed cells. It will be appreciated that such commercially available vector systems can easily be modified via commonly used recombinant techniques in order to replace, duplicate or mutate existing promoter or enhancer sequences and/or introduce any additional polynucleotide sequences such as for example, sequences encoding additional selection markers or sequences encoding reporter genes.
A vector according to the present invention, may, in another embodiment further include an appropriate selectable marker. The vector may further include an origin of replication, and may be a shuttle vector, which can propagate both in prokaryotic, and in eukaryotic cells, or the vector may be constructed to facilitate its integration within the genome of an organism of choice. The vector, in other embodiments may be, for example, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an artificial chromosome. In another embodiment, the vector is a viral particle comprising the nucleic acids of the present invention. In another embodiment, this invention provides liposomes comprising the nucleic acids and vectors of this invention. Methods for preparing such liposomes are well known in the art, and may be as described in, for example WO 96/18372; WO 93/24640; Mannino and Gould-Fogerite (1988) BioTechniques 6(7): 682-691; Rose U.S. Pat. No. 5,279,833; WO 91/06309; and Feigner et al. (1987) Proc. Natl. Acad. Sci. USA 84: 7413-7414).
In another embodiment, this invention provides an isolated polypeptide, comprising a secreted form of a TMEM27 protein, wherein said polypeptide is about 25 kDa in size, and comprises an N-terminal fragment of a sequence corresponding to, or homologous to: MLWLLFFLVTAIHAELCQPGAENAFKVRLSIRTALGDKAYAWDTNEEYLFKAMVAFSMRKVPN REATEISHVLLCNVTQRVSFWFVVTDPSKNHTLPAVEVQSAIRMNKNRINNAFFLNDQTLEFLKI PSTLAPPMDPSVPIWIIIFGVIFCIIIVAIALLILSGIWQRRRKNKEPSEVDDAEDKCENMITIENGIPS DPLDMKGGHINDAFMTEDERLTPL (SEQ ID NO: 16). In one embodiment, the secreted form comprises 142 amino acids. In another embodiment, the secreted form comprise the N-terminal 100 amino acids, or in another embodiment, the N-terminal 105 amino acids, or in another embodiment, the N-terminal 110 amino acids, or in another embodiment, the N-terminal 115 amino acids, or in another embodiment, the N-terminal 120 amino acids, or in another embodiment, the N-terminal 125 amino acids, or in another embodiment, the N-terminal 130 amino acids, or in another embodiment, the N-terminal 135 amino acids, or in another embodiment, the N-terminal 140 amino acids, or in another embodiment, the N-terminal 122 amino acids, or in another embodiment, the N-terminal 127 amino acids, or in another embodiment, the N-terminal 117 amino acids, or in another embodiment, the N-terminal 108 amino acids.
In one embodiment, the TMEM27 protein has a sequence corresponding to, or homologous to one disclosed in NCBI's Entrez protein database, having the Accession numbers: NP—065651, AAH50606, AAH15099, XP—416821, AAH49912, or AAH14317. In one embodiment, the polypeptides of this invention will comprise amino acids corresponding to those at positions 1-142 of a TMEM27 protein, or, in another embodiment, an N-terminal fragment thereof, or in another embodiment, a homologue thereof.
In one embodiment, the terms “homology”, “homologue” or “homologous”, in any instance, indicate that the sequence referred to, whether an amino acid sequence, or a nucleic acid sequence, exhibits at least 70% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 72% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 75% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 77% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 80% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 82% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 85% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 87% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 90% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 92% correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits at least 95% or more correspondence with the indicated sequence. In another embodiment, the amino acid sequence or nucleic acid sequence exhibits 95%-100% correspondence to the indicated sequence. Similarly, as used herein, the reference to a correspondence to a particular sequence includes both direct correspondence, as well as homology to that sequence as herein defined.
The term “polypeptide” refers, in one embodiment, to a protein or, in another embodiment, protein fragment, or, in another embodiment, a string of amino acids. In one embodiment, reference to “peptide” or “polypeptide” when in reference to any polypeptide of this invention, is meant to include native peptides (either degradation products, synthetically synthesized peptides or recombinant peptides) and peptidomimetics (typically, synthetically synthesized peptides), such as peptoids and semipeptoids which are peptide analogs, which may have, for example, modifications rendering the peptides more stable while in a body or more capable of penetrating into cells. Such modifications include, but are not limited to N terminal, C terminal or peptide bond modification, including, but not limited to, backbone modifications, and residue modification, each of which represents an additional embodiment of the invention. Methods for preparing peptidomimetic compounds are well known in the art and are specified, for example, in Quantitative Drug Design, C.A. Ramsden Gd., Chapter 17.2, F. Choplin Pergamon Press (1992).
It is to be understood that any amino acid sequence whether obtained naturally or synthetically, by any means, exhibiting sequence, structural, or functional homology to the polypeptides described herein are considered as part of this invention.
In one embodiment, the term “homology”, when in reference to any nucleic acid sequence indicates a percentage of nucleotides in a candidate sequence that are identical with the nucleotides of a corresponding native nucleic acid sequence.
Homology may be determined by computer algorithm for sequence alignment, by methods well described in the art. For example, computer algorithm analysis of nucleic acid or amino acid sequence homology may include the utilization of any number of software packages available, such as, for example, the BLAST, DOMAIN, BEAUTY (BLAST Enhanced Alignment Utility), GENPEPT and TREMBL packages.
An additional means of determining homology for nucleic acids is via determination of candidate sequence hybridization, methods of which are well described in the art (See, for example, “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., Eds. (1985); Sambrook et al., 1989, Molecular Cloning, A Laboratory Manual, (Volumes 1-3) Cold Spring Harbor Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y). For example methods of hybridization may be carried out under moderate to stringent conditions, to the complement of a DNA encoding a native caspase peptide. Hybridization conditions being, for example, overnight incubation at 42° C. in a solution comprising: 10-20% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7. 6), 5× Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA.
In another embodiment, this invention provides an antibody specifically recognizing a polypeptide of this invention. Production of such an antibody is well known in the art, and may comprise methods as described in, for example, Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, New York, 1988. In one embodiment, such antibodies may comprise functional fragments, which are well known to those skilled in the art, and may be prepared for example, by proteolytic hydrolysis of the antibody or by expression in E. coli or mammalian cells (e.g. Chinese hamster ovary cell culture or other protein expression systems) of DNA encoding the fragment.
In another embodiment, this invention provides compositions comprising the cells, polypeptides, antibodies, nucleic acids, and vectors of this invention. The effective dose and method of administration of a particular composition formulation can vary based on the particular application. Dosage may vary as a function of the type of vector, for example, employed and the route of administration.
In another embodiment, this invention provides cells comprising the nucleic acids, vectors, or polypeptides of this invention. In one embodiment, the cells are prokaryotic, or in another embodiment, the cells are eukaryotic. Cells may be any cells capable of expressing the isolated nucleic acids and/or vectors of this invention, as will be known to one skilled in the art. In one embodiment, cells may overexpress the polypeptides of this invention.
In another embodiment, cells are responsive to the polypeptides of this invention. In one embodiment, the cells are pancreatic β cells, or a pancreatic β cell, or a cell that may differentiate into a pancreatic β cell. In one embodiment, the cells, upon exposure to TMEM27, which, in one embodiment, is the full-length protein, or in another embodiment, is the cleaved secreted form, proliferate, or, in another embodiment, secrete insulin, or in another embodiment, do both. In one embodiment, the cells are terminally differentiated, or, in another embodiment, non-dividing. In another embodiment, the cells are dividing.
In one embodiment, this invention provides nucleic acids, vectors, polypeptides, cells, and compositions comprising the same, which may, in another embodiment, be formulated for oral or, in another embodiment, local administration, such as by aerosol, intramuscularly or transdermally, or via parenteral application. Compositions can be administered in a variety of unit dosage forms depending upon the method of administration. Suitable unit dosage forms, include, but are not limited to powders, tablets, pills, capsules, lozenges, suppositories, etc. Transdermal administration may be accomplished by application of a cream, rinse, gel, etc. capable of allowing the active compounds to penetrate the skin. Parenteral routes of administration may include, but are not limited to, electrical or direct injection such as direct injection into a central venous line, intravenous, intramuscular, intraperitoneal, intradermal, or subcutaneous injection.
TMEM27 expression was demonstrated herein in β-cells of the islet in newborn and adult pancreas (
In one embodiment, this invention provides a method of increasing pancreatic β-cell mass, where the method comprises contacting a cell with a TMEM27 polypeptide. According to this aspect of the invention, and in one embodiment, the cell is a pancreatic β-cell or said cell may be induced to become a pancreatic β-cell and providing conditions favorable for β-cell proliferation.
In one embodiment, the term “contacting a cell” refers to any exposure of a cell to a peptide, nucleic acid, or composition of this invention. Cells may be in direct contact with compounds and compositions of the invention, or exposed indirectly, through methods well described in the art. For example, cells grown in media in vitro, wherein the media is supplemented with polypeptides, nucleic acids, vectors or compositions of this invention would be an example of a method of contacting a cell, and considered a part of this invention. In another embodiment, contacting a cell may include any route of administration to a subject, for example, oral or parenteral administration of a peptide, nucleic acid, vector or composition of this invention to a subject, wherein administration results in in vivo cellular exposure to these materials, within specific sites within a body.
In one embodiment, the cells being contacted are primary cultures. In one embodiment, “primary culture” denotes a mixed cell population that permits interaction of many different cell types isolated from a tissue. For example, a primary culture of pancreatic duct cells may allow the interaction between mesenchymal and epithelial cells.
In another embodiment, a primary culture may be a purified cell population isolated from a tissue. In one embodiment, the primary culture may be enriched for a particular population. In one embodiment, enrichment may comprise cell sorting via means well known in the art, such as, for example fluorescent activated cell sorting (FACS), for cell populations, for example, expressing a particular cell surface marker, or in another embodiment, lacking cell surface expression of a particular marker. In another embodiment, manetoseparation methods may be used, or in another embodiment, density centrifugation methods, such as, for example, ficoll-hypaque or sucrose gradient separation.
In one embodiment, the cells contacted according to the methods of this invention are stem or progenitor cells. The term “progenitor cell” is used synonymously with “stem cell”, and refers, in some embodiments, to an undifferentiated cell which is capable of proliferation and giving rise to more progenitor cells having the ability to generate a large number of mother cells that can, in turn, give rise to differentiated, or differentiable daughter cells. In one embodiment, the term progenitor or stem cell refers to a generalized mother cell whose descendants (progeny) specialize, often in different directions, by differentiation, e.g., by acquiring completely individual characters, as occurs in progressive diversification of embryonic cells and tissues. Cellular differentiation is a complex process typically occurring through many cell divisions. A differentiated cell may derive from a multipotent cell which itself is derived from a multipotent cell, and so on. It is to be understood that any such cells may be used for the methods of this invention, or comprise the cells of this invention. Such capacity may be natural or may be induced artificially upon treatment with various factors, either scenario is to be considered as an embodiment of the cells which may be used according to the methods of this invention, or comprising a cell of this invention.
In one embodiment, cells of this invention and in use for the methods of this invention derive from the pancreas, or differentiate to a pancreatic cell. In one embodiment, the term “pancreas” refers generally to a large, elongated, racemose gland situated transversely behind the stomach, between the spleen and duodenum. In one embodiment, the cells of this invention may comprise tissue slices, or whole tissue, which comprise the polypeptides, nucleic acids, vectors or compositions, as described herein. In another embodiment, the methods of this invention include organ cultures, or, in another embodiment, tissue slices, or tissue fragments, comprising cell populations of interest. In one embodiment, islets of Langerhans are used as the cells and for the methods of this invention. In one embodiment, the cell is a β cell, and may constitute 60-80% of the islet cells, and/or may be used in the methods of this invention.
In one embodiment, the cell is a pancreatic progenitor cell. In one embodiment, the phrase “pancreatic progenitor cell” refers to a cell which can differentiate into a cell of pancreatic lineage, e.g. a cell which can produce a hormone or enzyme normally produced by a pancreatic cell. For instance, a pancreatic progenitor cell may be caused to differentiate, at least partially, into α, β, γ, or δ islet cell, or a cell of exocrine fate. The pancreatic of progenitor cells of the invention can also be cultured prior to administration to a subject under conditions which promote cell proliferation and differentiation. These conditions include culturing the cells to allow proliferation and confluence in vitro at which time the cells can be made to form pseudo islet-like aggregates or clusters and secrete insulin, glucagon, and/or somatostatin.
In one embodiment, the cells of this invention and for use in the methods of this invention, may be a substantially pure population. In one embodiment, the term “substantially pure”, refers to a population of cells that is at least about 65%, or, in another embodiment, at least about 70%, or, in another embodiment, at least about 75%, or, in another embodiment, at least about 85%, or, in another embodiment, at least about 90%, or, in another embodiment, at least about 95% pure, with respect to the total cell population.
Various techniques may be employed to obtain suspensions of cells (both differentiated and undifferentiated) from tissues, to comprise the cells of this invention and/or for use in the methods of this invention. In some embodiments, isolation procedures are ones that result in as little cell death as possible. For example, the cells can be removed from an explant sample by mechanical means, e.g., mechanically sheared off with a pipette. In other instances, it will be possible to dissociate the progenitor cells from the entire explant, or sub-portion thereof, e.g., by enzymatic digestion of the explant, followed by isolation of the activated progenitor cell population based on specific cellular markers, e.g., using affinity separation techniques or fluorescence activated cell sorting (FACS). Cells may be obtained from liquid samples, such as blood, by centrifugation.
In general, the tissue is prepared using any suitable method, such as by gently teasing apart the excised tissue or by digestion of excised tissue with collagenase (for example, collagenase A), via, to illustrate, perfusion through a duct or simple incubation of, for example, teased tissue in a collagenase-containing buffer of suitable pH and tonic strength. The prepared tissue may then, optionally, be concentrated using suitable methods and materials, such as centrifugation through Ficol gradients for concentration (and partial purification). The concentrated tissue then is resuspended into any suitable vessel, such as tissue culture glassware or plasticware. In certain embodiments, the sample pancreatic tissue is allowed to form a confluent monolayer culture, from which NACs are formed. In other preferred embodiments, the cell suspension is placed in a non-adherent culture container and spheres of progenitor cells are formed.
In one embodiment, tissue from which the cells are derived may be adult or fetal tissue or tissue from any developmental stage. Moreover, the method can be practiced with relatively small amounts of starting material. Accordingly, small samples of tissue from a donor can be obtained without sacrificing or seriously injuring the donor. The progenitor cells of the present invention can be amplified, and subsequently isolated from a tissue sample.
In certain embodiments, the culture may be contacted with a growth factor or a composition comprising a growth factor, e.g., a mitogenic growth factor, e.g., the growth factor is selected from a group consisting of IGF-I, IGF-II, LIF, TGFα, TGFβ, bFGF, aFGF, HGF or hedgehog. In other embodiments, the growth factor is a member of the TGFβ superfamily. In some embodiments, the methods of this invention comprise administering a composition to the subject, as described, wherein the composition may comprise a cell, polypeptide, nucleic acid and/or vector of this invention, further comprising any additive as herein described, including, for example, a diabetes treatment, a growth factor, a cAMP elevating agent, etc.
In some embodiments, the cells, or cells in culture are contacted with, or composition comprises, a cAMP elevating agents, such as 8-(4-chlorophenylthio)-adenosine-3′:5′-cyclic-monophosphate (CPT-cAMP) (see, for example, Koike Prog. Neuro-Psychopharmacol. and Biol. Psychiat. 16 95-106 (1992)), CPT-cAMP, forskolin, Na-Butyrate, isobutyl methylxanthine (IBMX) and cholera toxin (see Martin et al. J. Neurobiol. 23 1205-1220 (1992)) and 8-bromo-cAMP, dibutyryl-cAMP and dioctanoyl-cAMP (e.g., see Rydel et al. PNAS 85:1257 (1988)).
In some embodiments, the cells, or cells in culture are contacted with, or composition comprises, a steroid or corticosteroid such as, for example, hydrocortisone, deoxyhydrocortisone, fludrocortisone, prednisolone, methylprednisolone, prednisone, triamcinolone, dexamethasone, betamethasone and paramethasone. See, generally, The Merck Manual of Diagnosis and Therapy, 15th Ed., pp. 1239-1267 and 2497-2506, Berkow et al., eds., Rahway N.J., 1987).
There are a large number of tissue culture media that exist for culturing tissue from animals, including humans. In some embodiments, culture medium may be a simple medium, such as Dulbecco's Minimal Essential Media (DMEM). In another embodiment, cells, tissues, etc., are cultured in Isocove's modified MEM cell culture medium with 5% FBS. In another embodiment, cells, tissue, etc., are maintained in the absence of sera for extended periods of time. In some embodiments of the invention, the growth factors or other mitogenic agents are not included in the primary media for maintenance of the cultures in vitro, but are used subsequently to cause proliferation of distinct populations of cells, such as, for example, progenitor cells.
In another embodiment, stem cells are enriched because of their ability to grow in the absence of adherence, either direct or indirect, to a culture surface. In some embodiments, stem cells are free floating in suspension and are not in fixed or semi-fixed contact with a surface of the culture vessel or with other cells or materials that are in contact.
In one embodiment, the cells of this invention are for use in, and methods of this invention provide a source of implantable cells, either in the form of the progenitor cell population of the differentiated progeny thereof, or in another embodiment, the subject cells can be used to produce cultures of pancreatic cells for production and purification of secreted factors, such as the secreted form, for example, of TMEM27. In another embodiment, cultured cells can be provided as a source of insulin.
In another embodiment, this invention provides a method of increasing pancreatic β-cell mass, the method comprising contacting a cell with a nucleic acid encoding a TMEM27 polypeptide, wherein said cell is a pancreatic β-cell or said cell may be induced to become a pancreatic β-cell and providing conditions favorable for expression of said nucleic acid.
In one embodiment, increased β-cell mass occurs via increased proliferation of β-cells. In one embodiment, increased β-cell mass results from enhanced differentiation of precursor cells to a β-cell lineage. In one embodiment, increased β-cell mass refers to diminished cell turnover or death. In another embodiment, increased β-cell mass refers to any combination of the aforementioned embodiments.
Cell proliferation may be determined via any number of methods well known in the art, and as exemplified herein, for example via measuring uptake of a labeled substrate, such as tritiated thymidine, as will be known to one skilled in the art.
In another embodiment, this invention provides a method of altering metabolism in a subject, the method comprising contacting a cell with a TMEM27 polypeptide or a nucleic acid encoding said TMEM27 polypeptide, wherein said cell is a pancreatic β-cell or said cell may be induced to become a pancreatic β-cell.
In one embodiment, altering metabolism refers to increasing metabolism, while in another embodiment, it refers to decreasing metabolism. In one embodiment, glucose metabolism is altered. In one embodiment, the cell is contacted in vitro or ex vivo. In another embodiment, the cell is contacted in vivo. In another embodiment, the cell is a stem or progenitor cell. In another embodiment, the cell is isolated from a subject suffering from or predisposed to diabetes.
In one embodiment, the method comprises the step of administering the contacted cell to the subject, such as, for example, ex-vivo cellular therapy. In one embodiment, cells administered to a subject will be autologous or, in another embodiment, allogeneic with respect to the subject.
In one embodiment, the cells are contacted with a TMEM27 polypeptide which comprises a full-length protein, or a fragment thereof. In one embodiment, the fragment comprises a secreted form of a TMEM27 protein. In another embodiment, the secreted form is about 25 kDa in size, and comprises amino acids 1-142 of the TMEM27 protein, or a fragment thereof, or a sequence homologous thereto.
In one embodiment, the cells may also be contacted with a protease inhibitor. In one embodiment, a protease inhibitor is a molecule which represses, prevents, diminishes, delays, or in any way alters protease activity or expression or both.
In one embodiment, the protease inhibitor is a serine protease inhibitor (serpin), a metallo protease inhibitor, a cysteine protease inhibitor, a thio protease inhibitor, a trypsin inhibitor, a threonine protease inhibitor, an aspartic protease inhibitor, or a combination thereof.
In one embodiment, the protease inhibitor is (HONH-COCH2CH2CO-FA-NH2), (OA-Hy; cis-9-Octadecenoyl-N-hydroxylamide; Oleoyl-N-hydrocylamide), {N-[[(4,5-Dihydro-5-thioxo-1,3,4-thiadiazol-2-yl)amino]carbonyl]-L-phenylalanine Methyl Ester}, {a-[[[4,5-Dihydro-5-thioxo-1,3,4-thiadiazol-2-yl)amino]carbonyl]amino]-((2-pyridyl)piperazinyl)-(S)-benzenepropanamide}, {(2R)-2-[(4-Biphenylylsulfonyl)amino]-3-phenylpropionic Acid}, {(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide}, H-Cys1-Thr-Thr-His-Trp-Gly-Phe-Thr-Leu-Cys10-OH, (SB-3CT), (Ac-RCGVPD-NH2; Stromelysin-1 Inhibitor), [N-Isobutyl-N-(4-methoxyphenylsulfonyl)-glycylhydroxamic Acid; NNGH] {N-[[4,5-Dihydro-5-thioxo-1,3,4-thiadiazol-2-yl)amino]carbonyl]-L-phenylalanine}, {a-[[[4,5-Dihydro-5-thioxo-1,3,4-thiadiazol-2-yl)amino]carbonyl]amino]-N-(cyclohexylmethyl)-(S)-benzenepropanamide}, 4-Dibenzofuran-2¢-yl-4-hydroximino-butyric Acid), [4-(4¢-Biphenyl)-4-hydroxyimino-butyric-Acid], {(3R)-(+)-[2-(4-Methoxybenzenesulfonyl)-1,2,3,4-tetrahydroisoquinoline-3-hydroxamate]}, {(3S)-(−)-[2-(4-Methoxybenzenesulfonyl)-1,2,3,4-tetrahydroisoquinoline-3-hydroxamate]}, [N-Hydroxy-1-(4-methoxyphenyl)sulfonyl-4-(4-biphenylcarbonyl)piperazine-2-carboxamide], [N-Hydroxy-1-(4-methoxyphenyl)sulfonyl-4-benzyloxycarbonylpiperazine-2-carboxamide], (1,10-Phenanthroline Monohydrate), Marimastat, Prinomastat, Tanomastat, Neovastat, Zoledronic acid, or a combination thereof.
In one embodiment, the inhibitor is Alpha 1-antitrypsin, Alpha 1-antichymotrypsin, Alpha 2-antiplasmin (which in one embodiment, is an inhibitor of fibrinolysis), Antithrombin (which in one embodiment, is an inhibitor of coagulation, specifically factor X, factor IX and thrombin), Complement 1-inhibitor, Neuroserpin (which in one embodiment, is mutated in some familial forms of dementia), Plasminogen activator inhibitor-1 and 2 (which in one embodiment, is an inhibitor of fibrinolysis), Protein Z-related protease inhibitor (ZPI, which in one embodiment, inactivates factor Xa and factor XIa), or a combination thereof.
In another embodiment, the protease inhibitor is Leupeptin, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF), Aprotinin, Chymostatin, Antithrombin III, 3,4-Dichloroisocoumarin, L-1-chloro-3-[4-tosyl-amido]-7-amino-2-heptanone-HCl (TLCK), TPCK, diisopropyl phosphorofluoridate (DIFP), Antipain, 4-amidinophenylmethanesulfonyl-fluoride-HCl (APMSF), phenylmethanesulfonyl to fluoride (PMSF), 3,4-dichloroisocoumarin (DCI), α-toluenesulfonyl fluoride, BB94, α2-Macroglobulin, or a combination thereof.
In one embodiment, the protease inhibitor is EDTA, vanadium, molybdate salts, 1,10-Phenanthroline, Phosphoramidon, amastatin, Bestatin, actinonin, diprotin, BB94, α2-Macroglobulin, or a combination thereof.
In one embodiment, the protease inhibitor is N-Ethylmaleimide, Leupeptin, L-transepoxy-succinyl-leucyl-amido-(4-guanidino)-butane (E-64), Chymostatin, Antipain, α2-Macroglobulin, PMSF, PEFABLOC, or a combination thereof.
In one embodiment, the protease inhibitor is Pepstatin A, α2-Macroglobulin, or a combination thereof. In another embodiment, the protease inhibitor is BB94 or batimastat, which in one embodiment is a serine or metallo protease inhibitor. In one embodiment, the protease inhibitor is reversible, while in another embodiment, the protease inhibitor is irreversible.
In another embodiment, the cells may also be contacted with an inhibitor of PKC, which in one embodiment is Safingol (L-threo-dihydrosphingosine), Ro-1, Ro32-0432 (Bisindolylmaleimide tertiary amine), UCN-01 (7-OH-staurosporine), Flavopiridol (L86-8275), Bryostatin 1 (Macrocyclic lactone), Bisindolylmaleimide I, InSolution™ Bisindolylmaleimide I, Bisindolylmaleimide I, Hydrochloride, Bisindolylmaleimide II, Bisindolylmaleimide DI, Hydrochloride, Bisindolylmaleimide Inhibitor Set, Bisindolylmaleimide IV, Bisindolylmaleimide V, Calphostin C, Cladosporium cladosporioides, Cardiotoxin, Naja nigricollis, Chelerythrine Chloride, Dequalinium Chloride, Ellagic Acid, Dihydrate, G6976, InSolution™ G6976, G6983, G7874, Hydrochloride, H-7, Dihydrochloride, Iso-H-7, Dihydrochloride, HBDDE, Hispidin, Hypericin, K-252a, Nocardiopsis sp., InSolution™ K-252a, Nocardiopsis sp., K-252b, Nocardiopsis sp., K-252c, Melittin, NGIC-I, Phloretin, Piceatannol, PKCb11/EGFR Inhibitor, Staurosporine, N-Benzoyl PKCb Inhibitor, Polymyxin B Sulfate, Protein Kinase C Inhibitor 20-28, Cell-Permeable, Myristoylated, Protein Kinase C Inhibitor Peptide 19-31, Protein Kinase C Inhibitor Peptide 19-36, Protein Kinase C Inhibitor Set, Protein Kinase C Inhibitor, EGF-R Fragment 651-658, Myristoylated, Protein Kinase Ce Translocation Inhibitor Peptide, Protein Kinase Ce Translocation Inhibitor Peptide, Negative Control, Protein Kinase Cz Pseudosubstrate Inhibitor, Protein Kinase Cz Pseudosubstrate Inhibitor, Myristoylated, Protein Kinase Ch Pseudosubstrate Inhibitor, Myristoylated, Protein Kinase Cq Pseudosubstrate Inhibitor, Protein Kinase Cq Pseudosubstrate Inhibitor, Myristoylated, Pseudohypericin, Ro 31-7549, Immobilized, Ro-31-7549, Ro-31-8220, InSolution™ Ro-31-8220, Ro-31-8425, Ro-32-0432, Rottlerin, Safmgol, Sangivamycin, Scytonemin, Lyngbya sp., Serine/Threonine Kinase Inhibitor Set, D-erythro-Sphingosine, Dihydro-, D-erythro-Sphingosine, Free Base, Bovine Brain, D-etythro-Sphingosine, Free Base, High Purity, D-etythro-Sphingosine, N,N-Dimethyl-, Staurosporine, Streptomyces sp., Tamoxifen Citrate, Tamoxifen, 4-Hydroxy-, (Z)-, TER14687, Vitamin E Succinate, or antisense nucleotides capable of inhibiting the expression of the protein kinase C.
In one embodiment, the protease comprise trypsin, chymotrypsin and elastase. In one embodiment, cysteine proteases comprise papain, calpain and lysosomal cathepsins. In one embodiment, aspartic proteases include pepsin and rennin. In one embodiment, Metallo-proteases include thermolysin and carboxypeptidase A.
In one embodiment, the compositions and/or methods of this invention may comprise use of any combination of inhibitors as herein described.
In another embodiment, the cells may also be contacted with a Tcf1 protein or a nucleic acid encoding said Tcf1 protein.
In one embodiment, the Tcf1 protein may have an amino acid sequence, which is homologous to, or as set forth in NCBI's Genbank, Accession Number: CAI59557, P15257, P22361, P20823, NP—033353, NP—036801, NP—739570 or NP—000536.
In another embodiment, this invention provides a method of increasing pancreatic β-cell mass, the method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a serine or metallo protease inhibitor, wherein said cell expresses or may be induced to express TMEM27 and providing conditions favorable for β-cell proliferation.
In another embodiment, this invention provides a method of altering metabolism in a subject, the method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a serine or metallo protease inhibitor, wherein said cell expresses or may be induced to express TMEM27.
In another embodiment, this invention provides a method of inhibiting, suppressing or treating diabetes in a subject, the method comprising contacting a cell with a TMEM27 polypeptide or a nucleic acid encoding said TMEM27 polypeptide, wherein said cell is a pancreatic β-cell or said cell may be induced to become a pancreatic β-cell.
In another embodiment, this invention provides a method of inhibiting, suppressing or treating diabetes in a subject, the method comprising contacting a pancreatic β-cell or a cell which may be induced to become a pancreatic β-cell with a protease inhibitor with a serine or metallo protease inhibitor, wherein said cell expresses or may be induced to express TMEM27.
In one embodiment, “treating” refers to both therapeutic treatment and prophylactic or preventative measures, wherein the object is to prevent or lessen the targeted pathologic condition or disorder as described hereinabove. Thus, in one embodiment, treating may include suppressing, inhibiting, preventing, treating, or a combination thereof. Thus, in one embodiment, “treating” refers inter alia to increasing time to sustained progression, expediting remission, inducing remission, augmenting remission, speeding recovery, increasing efficacy of or decreasing resistance to alternative therapeutics, or a combination thereof. In one embodiment, “suppressing” or “inhibiting”, refers inter alia to delaying the onset of symptoms, preventing relapse to a disease, decreasing the number or frequency of relapse episodes, increasing latency between symptomatic episodes, reducing the severity of symptoms, reducing the severity of an acute episode, reducing the number of symptoms, reducing the incidence of disease-related symptoms, reducing the latency of symptoms, ameliorating symptoms, reducing secondary symptoms, reducing secondary infections, prolonging patient survival, or a combination thereof.
In one embodiment, symptoms are primary, while in another embodiment, symptoms are secondary. In one embodiment, “primary” refers to a symptom that is a direct result of diabetes, while in one embodiment, “secondary” refers to a symptom that is derived from or consequent to a primary cause. In one embodiment, the compounds for use in the present invention treat primary or secondary symptoms or secondary complications related to diabetes.
In another embodiment, “symptoms” may be any manifestation of a disease or pathological condition, which in one embodiment is diabetes, comprising Frequent urination, Excessive thirst, Extreme hunger, Unusual weight loss, Increased fatigue, Irritability, Blurry vision, low insulin levels, high blood or urinary glucose levels or a combination thereof.
In one embodiment, the term “diabetes” refers to a disease of a mammalian subject, with primary, or in another embodiment, secondary diabetes, or in another embodiment, type 1 NIDDM-transient, or in another embodiment, type 1 IDDM, or in another embodiment, type 2 IDDM-transient, or in another embodiment, type 2 NIDDM, or in another embodiment, type 2 MODY, which may manifest, as described, in Harrison's Internal Medicine, 14th ed. 1998.
According to this aspect of the invention, and in one embodiment, the subject is insulin resistant or, in another embodiment, hypoinsulinemic. In another embodiment, the subject is predisposed to diabetes. In another embodiment, the subject has maturity onset diabetes of the young (MODY).
In another embodiment, the methods of this invention may further comprise the step of administering to the subject an additional diabetes medication, as part of a combination therapy. In one embodiment, the diabetes medication may comprise a sulfonylurea, leptin, meglitinide, biguanide, thiazolidinedione, alpha-glucosidase inhibitor, or a combination thereof.
In another embodiment, the methods of this invention may further comprise administering to the subject, or in another embodiment, contacting cells in the subject, with a GLP-1 receptor agonist. In one embodiment, the GLP-1 agonist may include naturally occurring peptides such as GLP-1, exendin-3, and exendin-4 (see, e.g., U.S. Pat. No. 5,424,286; U.S. Pat. No. 5,705,483, U.S. Pat. No. 5,977,071; U.S. Pat. No. 5,670,360; U.S. Pat. No. 5,614,492), GLP-1 analogs or variants (see, for example, U.S. Pat. No. 5,545,618 and U.S. Pat. No. 5,981,488), and small molecule analogs. GLP-1 receptor agonists may be tested for activity as described in U.S. Pat. No. 5,981,488.
In another embodiment, the methods of this invention may further comprise administering PDX-1, or PYY, or a nucleic acid encoding the same.
It is to be understood that any embodiments described herein, with regards to a cell, composition, polypeptide, nucleic acid or vector of this invention, may be used in a method of this invention, and is to be considered an embodiment of this invention.
In one embodiment, this invention provides a method of assessing pancreatic islet mass in a subject, the method comprising the step of determining a concentration of TMEM27 in the serum of said subject and comparing said concentration versus that of a control.
In another embodiment, this invention provides a diagnostic tool and/or method for assessing hyperinsulinemia, hypoinsulinemia, diabetes or response to therapies for the treatment of the aforementioned conditions. In one embodiment, TMEM27 serves as a biomarker, whose concentration in a given body fluid, such as, for example, in serum, plasma or urine is a function of hyperinsulinemia, hypoinsulinemia or frank diabetes. In one embodiment, the secreted form of TMEM27 is a biomarker. According to this aspect of the invention, and in , one embodiment, a method of diagnosis may be to determine levels of the TMEM27 protein, or fragments, secreted forms thereof, via standard assays, such as, for example, an ELISA assay. In one embodiment, disease severity is diagnosed as a function of the expression of the polypeptide. In another embodiment, changes in expression of the polypeptide, either at the protein or RNA level, may be a function of, in another embodiment, response to treatment, such as the treatment methods provided herein. In another embodiment, relative changes in expression may be assessed as a function of delivery of a nucleic acid or vector of this invention, which may represent a therapy of this invention. In one embodiment, the methods of this invention may employ the use of an antibody or antibody fragment, or composition comprising the same, of this invention. A kit comprising the antibody, nucleic acids, vector, cell and/or compositions of this invention are also encompassed by this invention.
In another embodiment, this invention provides a method for identifying proteins, which interact with a TMEM27 protein, or a secreted form thereof, the method comprising the step of contacting a TMEM27 polypeptide with a molecule of interest for a time and under conditions sufficient to facilitate specific interaction between said polypeptide and said molecule of interest and identifying said molecule of interest.
In one embodiment, TMEM27 may function as a hormone, and an interacting protein may comprise an unidentified receptor which is responsive to the hormone. In one embodiment, the interacting protein may comprise a known receptor involved in a metabolic pathway in a subject.
In one embodiment, such screening methods are well known in the art, and may comprise direct or indirect assessment of interactions between the two, such as, for example, analysis under denaturing versus non-denaturing conditions for gel electrophoresis, use of chemical crosslinking agents, or other molecular means, such as, for example, use of the yeast 2 hybrid system.
It is to be understood that any means of determination of interacting partners with a TMEM27 protein of this invention may be accomplished and considered as a part of this invention, and is meant to include identification of the interacting partner, as well as a role for such a partner in the conditions described herein, for example, positive metabolic effects, such as their effect on enhancing pancreatic β-cell mass, or in another embodiment, deleterious metabolic effects, for example, such as the development of hyperinsulinemia.
The following are meant to provide materials, methods, and examples for illustrative purposes as a means of practicing/executing the present invention, and are not intended to be limiting.
All animal models were housed in Laboratory of Animal Research Center (LARC), a pathogen-free animal facility at the Rockefeller University. The animals were maintained on a 12 hours light/dark cycle and fed a standard rodent chow. Genotyping of mutant mice was performed on DNA isolated from 3 weeks old mice by PCR.
Two peptides: anti-Col-3: VQSAIRKNRNRINSAFFLD (SEQ ID NO: 1) and anti-Col-4: GIPCDPLDMKGGHINDGFLT (SEQ ID NO: 2) were synthesized and processed to >90% purity, conjugated to KLH and used for immunization of rabbits (Bethyl Laboratories, Texas). Antisera were affinity purified and tested by western blotting and immunohistochemistry. Affinity purified antisera were used for all studies. Other antibodies used for immunoblotting and immunohistochemistry were obtained from the following sources: anti-insulin (Linco), anti-glucagon (Linco), anti-V5 (Invitrogen), Rhodamine red conjugated donkey anti-guinea pig (Jackson Labs), Alexa 488 donkey anti-rabbit (Molecular Probes).
MIN6 cells were cultured with DMEM medium containing 25 mM glucose, 15% fetal bovine serum, and 5.5 μM 2-mercaptoethanol. INS-1 cells were cultured with RPMI 1640 medium containing 25 mM glucose and 5% fetal bovine serum and 10 mM Hepes pH7.4. HepG2 cells were cultured with DMEM medium containing 25 mM glucose and 10% fetal bovine serum.
Fugene reagent (Roche) for HepG2 cells and Lipofectamine 2000 (Invitrogen) for MIN6 cells were used according to the manufacturer's directions in transient transfections. 0.5 μg of luciferase reporter construct, expression vector and CMV-LacZ were added per 35 mm dish. Luciferase was normalized for transfection efficiency by the corresponding 13-galactosidase activity [Alam J., Cook, J. L.: Reporter genes: Application to the study of mammalian gene transcription. Anal. Biochem. 188:245-254, 1990].
For the luciferase construct, an 815 by promoter region was cloned upstream of a luciferase promoter and coexpressed with a Tcf1 expression vector. The TMEM27 promoter sequence (mouse) was as follows:
In addition, the TMEM27 promoter was selectively mutated for Tcf1 sites, called the M1 and M2 promoter sequences, respectively, as follows:
Sequence of TMEM27-M1 Promoter (Mouse):
Sequence of TMEM27-M2 Promoter (Mouse):
MIN6 cells, grown to 90% confluency in 6 well plates, were harvested and resuspended in reaction buffer and reagent in 50 μl volume and incubated for 2 hrs at 4° C. Reactions were stopped by adding 50 mM Tris pH 7.4 for 10 min on ice. Cells were then washed with PBS and lysed in RIPA buffer in the presence of protease inhibitors for 15 min at 4° C. Cell lysates were centrifuged for 5 min at 10,000×g and the supernatants were subjected to reducing and non-reducing SDS-PAGE followed by immunoblotting. Crosslinking reagents and buffers: BMH (Pierce) was dissolved in DMSO and incubated in PBS, and DTBP (Pierce) was dissolved in water and incubated in 0.2 M triethanolamine pH 8.0.
MIN6 cells that express pV5-TMEM27 were incubated with indicated concentrations of tunicamycin (Sigma) dissolved in DMSO and with DMSO alone for 12 hours at 37° C. Cell lysates were then subjected to SDS-PAGE and immunoblotting with anti-V5 antibody.
Intact N-linked glycans were removed from secreted portion of TMEM27 with the recombinant enzyme N-glycanase (Prozyme, San Leandro, Calif.). Supernatants from MIN6 cells and pancreatic islets were denatured in 20 mM sodium phosphate pH 7.5, 0.1% SDS and 50 mM β-mercaptoethanol by heating at 100° C. for 5 min. NP-40 was added to a final concentration of 0.75% and reaction was incubated with N-glycanase for 3 h at 37° C.
EMSA analysis was performed with 10 μg of whole cell extracts in binding buffer (20 mM Hepes pH 7.9, 10% glycerol, 150 mM NaCl, 1 mM DTT). Whole cell extracts were incubated with 32P-labeled ds-stranded oligonucleotide probes containing the wildtype or mutant HNF-1 binding sites in the TMEM27 promoter (sequences: 5′-GGAGATTTTCGTAAATAACTGACA-3′ (SEQ ID NO: 3), 5′-GGGCGTTAATTATTAAACCTTTTA-3′ (SEQ ID NO: 4) and 5′-GGGCAGAGATTATTAAACCTTTTA-3′ (SEQ ID NO: 5), respectively). Supershifts were carried out with an anti-Tcf1 antibody (Geneka Biotechnology Inc., Montreal, Canada). ChIP analysis was carried out using isolated primary hepatocytes from C57/B6 mice or MIN6 cells, and the ChIP Assay kit (Upstate Cell Signaling Solutions, Lake Placid, N.Y.) according to the manufacturer's protocol. Tcf1 was precipitated with anti-HNF-1α antibody, and DNA was amplified using primers x and y (sequences: 5′-ACAGGAGGCAGGTGGGAGGCTTCT-3′ (SEQ ID NO: 6) and 5′-CCCGGATTAGGGTATCGGAGAA-3′) (SEQ ID NO: 7). Primers for apoM were 5′-GGGCTCAGCTTTCCTCCTA-3′ (SEQ ID NO: 8) and 5′-CTCCGCCTTAACTGTTCTCTGATG-3′) (SEQ ID NO: 9).
MIN6 cells were grown to 90% confluency in 150 mm tissue culture dishes. Cells were washed once in ice-cold PBS and scraped into 3 ml PBS. Cells were centrifuged at 4,000×g for 4 min and resuspended in 2 volumes of high-salt extraction buffer (400 mM KCl, 20 mM Tris pH 7.5, 20% glycerol, 2 mM DTT, 1× complete TM protease inhibitors (Boehringer Mannheim), and 20 μg/mL Aprotinin). Cell lysis was performed by freezing and thawing, and the cellular debris was removed by centrifugation at 16,000×g for 10 min at 4° C.
Insulin and glucagon were extracted from pancreata with acid ethanol (10% glacial acetic acid in absolute ethanol), sonicated for 10 min, and centrifuged 2 times at 4° C. at 12,000×g for 10 min. Supernatants were collected and stored at −20° C. for insulin determination by using an sensitive insulin or glucagon RIA kit (Linco Research).
Pancreatic islets were isolated from 6 to 8 week-old mice. We used collagenase digestion and differential centrifugation through Ficoll gradients. Total RNA was then extracted using TRIzol reagent (Gibco-BRL) and following the manufacturer's instructions. Contaminating genomic DNA was removed using 1 μl of RNase free DNase-I (Boehringer) per 5 μg of RNA.
The pancreata were fixed in paraformaldehyde and stained for insulin and glucagon as described above. Sections (7 μm) through the entire pancreas were taken, and every sixth section was used for morphometric analysis. At least 288 non-overlapping images (pixel size 0.88 μm) were scanned using a to confocal laser-scanning microscope (Zeiss LSM 510, Germany). The parameters measured in this study were analyzed using integrated morphometry analysis tool in the Metamorph Software Package (Universal Imaging Corporation, PA). The area covered by cells stained by insulin or glucagon was integrated using stained objects that are greater than 3 pixel in size.
Total RNA was extracted using TRIZOL reagent (Invitrogen) and 10 μg of RNA was treated with 5U of RNase-free DNase-I (Ambion). cDNA was synthesized using Moloney leukemia virus reverse transcriptase with dNTPs and random hexamer primers (Invitrogen). The cDNAs provided templates for PCRs using specific primers in the presence of [α-32P]dCTP and Tact polymerase as previously described [Shih D Q, Screenan S, Munoz K N, Philipson L, Pontoglio M, Yaniv M, Polonsky K S, Stoffel M. (2001) Loss of HNF-1alpha function in mice leads to abnormal expression of genes involved in pancreatic islet development and metabolism. Diabetes 50:2472-80].
Cytosolic protein extracts were separated by SDS-PAGE (4-15%) and transferred onto a nitrocellulose membrane (Schleicher & Schuell) by electroblotting. TMEM27 was detected with anti-Col3 and anti-Col4 antisera (1:500). Membranes were incubated with primary antibodies overnight at 4° C. Incubations containing the secondary antibody were performed at RT for 1 hr. Non-reducing SDS-PAGE was performed by omitting DTT from sample buffer.
MIN6 cells were plated on coated slides (Nalge Nunc Int.) and fixed with 4% paraformaldehyde at 4° C. for 20 min. Slides were incubated in 0.01% saponin with 3% normal donkey serum in PBS for 30 min at room temperature and anti-Col3 and anti-Col4 (1:20) were added overnight at 4° C. Secondary antibody was added for 30 min at RT.
Pancreata were fixed in 4% paraformaldehyde at 4° C. for 4 hours and embedded in paraffin. Seven μm sections were cut and after deparaffinization, antigen-unmasking was performed by microwaving slides in 0.01 M sodium citrate pH 6.0. Sections were permeabilized in 0.1% Triton-X-100 and incubated in 3% normal donkey serum in PBS for 30 min. Staining was completed as above.
Min 6 cells were fixed in 4% paraformaldehyde and 0.02% glutaraldehyde in 0.1M cacodylate buffer, pH 7.4 for 2 hours. The cells were washed with PBS and embedded in 10% gelatin and refixed as above. Cell pellets were cryo-protected using a 2.3M sucrose solution in PBS, and samples were stored in to liquid nitrogen until use (Tokuyasu, K. T. 1973. J. Cell. Biol. 57-551-565). Cryo-ultrathin sections were cut using glass knives in a Reichert-Jung FC-4E cryoultramicrotome. The sections were collected on Formvar-carbon coated nickel grids, blocked with 1% BSA-PBS and incubated with anti-col4 at a 1:10 dilution. Incubation was stopped by washing 2 times with PBS, 15 min each. The sections were then incubated with goat anti-rabbit IgG conjugated to 10 nm gold particles (Amersham Life Science, Arlington Heights, Ill.). The grids were processed and stained according to Griffiths et al. 1983. Methods Enzymol. 96:466-485).
[3H]Thymidine incorporation in 5×104 cells/well in 24-well culture plates was assayed as follows. 48 hours after electroporation, cells were incubated in growth arrest medium (0.5% FCS) for the next 24 hours and then incubated for an additional 24 hours in normal growth medium. For the last 4 h of the incubation, 0.25 μCi/well [3H]methylthymidine (Perkin Elmer) was added. After completion, cells were rinsed twice in ice-cold PBS, and incubated with 10% trichloroacetic acid (TCA) on ice for 20 min. After washing with 10% TCA, cells were solubilized in 0.2M NaOH/1% SDS for 10 min at room temperature. TCA-insoluble materials were neutralized with 0.2M HCl, and radioactivity was determined by a liquid scintillation counter.
Synthetic siRNAs were synthesized by Dharmacon Research (Lafayette, Colo.). siRNAs were designed for mouse TMEM27 (NM—020626), PC1/3 (NM—013628), PC2 (NM—008792), furin (NM—011046), and carboxypeptidase E (NM—013494) sequences. 3 μg of each siRNA per 1×106 cells were electroporated into MIN6 cells.
Results are given as mean ±SD. Statistical analyses were performed by using a Student's t-test, and the null hypothesis was rejected at the 0.05 level.
In an attempt to identify mitogenic factors in pancreatic β-cells, gene expression in isolated pancreatic islets of Tcf1-/- wildtype littermates was compared using Affymetrix™ oligonucleotide expression arrays. This analysis identified a gene encoding a transmembrane-spanning protein (TMEM27) that showed a 16-fold decrease in expression levels of Tcf1-/- mice compared to controls. This result was confirmed by RT-PCR in an independent group of animals (
An 815 by promoter region (SEQ ID NO: 17) was cloned upstream of a luciferase promoter and coexpressed with a Tcf1 expression vector. Transient co-transfection of Tcf1 and the TMEM27 promoter led to a >5-fold activation of luciferase activity (
Immunohistochemical analysis indicated the pattern of expression of TMEM27 during mouse pancreatic development. In newborn and adult pancreas, TMEM27 expression was restricted to β-cells of the islet. During development of the pancreas, TMEM27 was expressed at the earliest time when hormone positive (mainly glucagon-positive cells) are apparent. At embryonic days E11.5 and E12.5, TMEM27 expression was localized in cells that express glucagon and insulin. During the peak period of endocrine cell expansion, from embryonic day 13.5 to 18.5, TMEM27 mainly co-localized with glucagon until right before birth at E18.5 when it was also detected in insulin positive cells. In newborn and adult pancreas, TMEM27 was no longer detected in cc-cells of the islets and expression was confined to β-cells (
TMEM27 contains two predicted N-glycosylation sites at amino acid residues 76 and 93. MIN6 cells were treated with tunicamycin, which inhibits N-glycosylation. Cells were incubated in the presence of increasing concentrations of tunicamycin, and protein extracts were prepared and analyzed by SDS-PAGE and immunoblotting. Two bands with increased electrophoretic mobility appeared upon treatment with tunicamycin, whereas the high molecular weight protein disappeared (
Chemical cross-linking experiments testing whether TMEM27 protein exists as a multimer were conducted. MIN6 cell extracts were incubated in the presence of two different cross-linking reagents; BMH (bismaleimidohexane), a membrane-permeable, non-cleavable compound and DTBP (dimethyl 3,3′-dithiobispropionimidate), a membrane permeable, cleavable compound prior to SDS-PAGE analysis that was performed under reducing and non-reducing conditions. Non-reducing western blots revealed a band of exactly twice the molecular weight than the TMEM protein in western blots under reducing conditions. Cell extracts treated with BMH showed the dimer protein in both reducing and non-reducing blots. However, the protein dimers disappeared under reducing conditions in the presence of DTBP (
Taken together, these data indicated that the TMEM27 protein is N-glycosylated, and exists as a dimer.
TMEM27 was predicted to be a transmembrane protein with an N-terminal extracellular domain. Two antibodies (α-Col-3 and α-Col-4) that recognize peptides from extra- and intracellular domains of the protein, respectively, were generated. In Western blotting experiments, α-Col-4 detected two bands in whole cell lysates of MIN6 cells (
In order to demonstrate whether the 25 kD band represented a C-terminal part of a cleaved form of TMEM27 and/or whether it was secreted, supernatants from different cell lines (including neuronal N2A cells, HepG2c ells, Hek293 and M-1 cells that were derived from collecting ducts of the kidney and which exhibit endogenous expression of TMEM27) were probed with the α-Col-3 antibody. Cells were cultured for 48 hours in serum free medium, and probed by Western blotting using α-Col-3, with a band corresponding to a ≈25 kDa protein detected in the clonal β-cell lines MIN6 and INS1E (
In contrast, overexpression of TMEM27 in MIN6 and INS1E cells led to increased amounts of secreted protein in their respective culture media. siRNA-mediated reduction in TMEM27 protein levels correlated with decreased levels of the secreted form of TMEM27 in MIN6 cell supernatants (
Immunofluorescence studies using anti-Col-3 and -4 antibodies demonstrated TMEM27 localization in pancreatic β-cells. MIN6 cells were grown on slides, fixed, permeabilized and treated with primary antibodies. Specific staining was detected on the plasma membrane as well as in the perinuclear compartment and in granules (
TMEM27 expression in hypertrophied islets of ob/ob and aP2-Srebp-1c transgenic mice was evaluated to determine relative expression, as compared to controls. Pancreatic islets from 6-8 week old mice were isolated and gene expression levels were compared to their wildtype littermates. A 2.1- and 1.7-fold increase in TMEM27 levels of ob/ob and aP2-Srebp-1c islets, respectively, was found in semi-quantitative RT-PCR assays, in comparison to control values (
Pancreatic β-cell-specific transgenic mice were generated via pronuclear microinjection of a vector construct in which the murine TMEM27 cDNA was under the control of the rat insulin promoter. RT-PCR and immunoblot analysis showed that TMEM27 expression was ≈4-fold increased in transgenic mice compared to wildtype littermates (
The pancreatic beta-cell line MIN6 was cultured with DMEM medium containing 25 mM glucose, 15% fetal bovine serum, and 5.5 μM 2-mercaptoethanol. MIN6 cells were plated at 50% confluency in 12 well plates and incubated for 24 hrs. Cells were washed with OPTI-MEM (Invitrogen) and incubated with either DMSO or protease inhibitor BB94 (1 μM) for 15 minutes in OPTI-MEM at 37° C. After the above incubation, cells were incubated with DMSO, PMA (Sigma P8139), BB94, or PMA+BB94 for 2 hrs in OPTI-MEM. 30 μl of each supernatant was collected for SDS-PAGE. Immunoblots were performed using anti-TMEM27 antibodies recognizing the N-terminus (cleaved portion) of TMEM27 (
While certain features of the invention have been illustrated and described herein, many modifications, substitutions, changes, and equivalents will now occur to those of ordinary skill in the art. It is, therefore, to be understood that the appended claims are intended to cover all such modifications and changes as fall within the true spirit of the invention.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/US06/22211 | 6/7/2006 | WO | 00 | 1/13/2010 |
| Number | Date | Country | |
|---|---|---|---|
| 60687856 | Jun 2005 | US | |
| 60741893 | Dec 2005 | US |
