Patent Application
20200187402
Genus and species: The strawberry cultivar of this invention is botanically identified as Fragaria x ananassa Duchesne.
Variety denomination: The variety denomination is ‘UCD Royal Royce’.
This invention relates to a new and distinct day-neutral strawberry cultivar designated as ‘UCD Royal Royce’, which originated from a cross performed in the winter of 2008 between proprietary germplasm parents 03C114P003 (unpatented) and 04C009P005 (unpatented). Seeds of the cross were harvested from greenhouse-grown plants in the spring of 2008 and germinated in June 2008. Seedlings were transplanted to a greenhouse in July 2008 and transplanted to the field in October 2008. ‘UCD Royal Royce’ was selected and clones were first harvested in 2009. ‘UCD Royal Royce’ has been asexually propagated since 2009.
The plant of this selection was originally designated ‘08C123P001’ and later called ‘16DN009’ or ‘UC9’ for evaluation in field trials.
‘UCD Royal Royce’ is a day-neutral (ever-bearing) strawberry cultivar selected for increased marketable fruit yield, extended shelf-life and decreased runner (stolon) production. ‘UCD Royal Royce’ consistently produces more marketable fruit per hectare, particularly during the first six to seventh months of an eight or nine-month harvest season, compared to San Andreas (U.S. Plant Pat. No. 19,975), ‘Monterey’ (U.S. Plant Pat. No. 19,767) or ‘Cabrillo’ (U.S. Plant Pat. No. 27,830); and has fewer stolons (runners) than ‘Monterey’ or ‘Cabrillo’. ‘UCD Royal Royce’ also delivers yield earlier than cultivar ‘UCD Moxie’ (U.S. plant patent application filed of even date under attorney docket number 069516-1131471-231000US), whereas the cumulative marketable fruit yield of ‘UCD Moxie’ surpasses that of ‘UCD Royal Royce’ late in the season.
‘UCD Royal Royce’ was genotyped with a 35,000-SNP array (Hardigan et. al., Plant Genome 11:180049, 2018). The variety has a unique DNA profile compared to ‘Cabrillo’, ‘Monterey’, ‘UCD Moxie’, and ‘UCD Valiant’ (U.S. plant patent application filed of even date under attorney docket number 069516-1131474-232000US). The pairwise genetic distances between ‘UCD Royal Royce’ and ‘UCD Valiant’, ‘UCD Moxie’, ‘Cabrillo’, and ‘Monterey’ are estimated to be 0.270, 0.167, 0.259, and 0.302, respectively based on the 35,000-SNP genotype analysis.
‘UCD Royal Royce’ is maintained by annual asexual propagation by stolons in Winters, Calif.
The colors in the photograph are depicted as nearly true as is reasonably possible to obtain in color reproductions of this type.
‘UCD Royal Royce’ and comparison cultivars were asexually propagated in high-elevation nurseries in Dorris and Mcdoel, Calif. for field testing in 2015-2016, 2016-2017, and 2017-2018. Clones were harvested according to commercial planting schedules, which were calibrated by the number of chill hours required for optimum production in Santa Maria and Prunedale, Calif.
Cultivar testing in small-plot yield trials was performed as follows. Test day-neutral cultivars and comparison cultivars were field tested in Oso Flaco (Santa Maria, Calif.) and Prunedale, Calif. in 2015-2016. Fruit was harvested once or twice per week over the spring and summer growing season: Mar. 16 to Aug. 18, 2016 in Santa Maria (31 harvests) and Apr. 2 to Aug. 27, 2016 in Prunedale (30 harvests). ‘UCD Royal Royce’ was selected on the basis of fruit appearance, size, shelf-life, and cumulative marketable fruit yield for a second year (2016-17) of replicated testing in Santa Maria and Prunedale (Tables 1-2). Fruit was harvested once or twice per week over the growing season: Mar. 13 to Aug. 21, 2017 in Santa Maria (25 harvests) and Apr. 3 to Sep. 1, 2017 in Prunedale (44 harvests in Prunedale). To highlight differences among cultivars, the yield data were displayed in three ways: (a) per plant yields for individual harvests for each location×year combination for ‘UCD Royal Royce’, ‘Monterey’, ‘San Andreas’, and ‘Cabrillo’; (b) per plant cumulative marketable yields for ‘UCD Royal Royce’, ‘Monterey’, ‘San Andreas’, and ‘Cabrillo’; and (c) per plant cumulative marketable yields tested in both years and locations. Entry ranks were highly consistent and the best and worst performing cultivars were virtually identical over locations and years.
Table 1 shows marketable yield (g/plant) for ‘UCD Royal Royce’ compared to that for ‘Monterey’, ‘San Andreas’, and ‘Cabrillo’ tested in small-plot yield trials in Santa Maria, Calif. and Prunedale, Calif. in 2015-16 and 2016-17.
Table 2 shows the range in increase in yield compared to ‘Monterey’, ‘San Andreas’, and ‘Cabrillo’ for the small-plot yield trials.
The cumulative marketable fruit yields of ‘UCD Royal Royce’ were significantly greater than ‘Cabrillo’ (p<0.0078), ‘Monterey’ (p<0.0001), and ‘San Andreas’ (p<0.0001) across years and locations (Table 1). The per plant yields of ‘UCD Royal Royce’ were 12 to 112% greater than ‘Cabrillo’, ‘Monterey’, and ‘San Andreas’ (Table 2). The mean fruit size for ‘UCD Royal Royce’ (29.7 g/fruit) was not significantly different from ‘Cabrillo’ (30.0 g/fruit; p=0.78) or Monterey (28.4 g/fruit; p=0.23), but was significantly greater than ‘San Andreas’ (26.7 g/fruit; p=0.007).
The marketable fruit percentages for ‘UCD Royal Royce’ and ‘Cabrillo’ were comparable (86-88%) and superior to ‘Monterey’ and ‘San Andreas’ (76 to 84%) (Table 2).
Significantly less runner production was observed in ‘UCD Royal Royce’ compared to the other high yielding cultivars tested in small-plot yield trials. Runner production differences among cultivars could not be absolutely quantified because growers trimmed runners to prevent the diversion of energy away from fruit production; however, it is estimated based on observation that runner production was reduced by at least 50% for ‘UCD Royal Royce’ compared to runner production by ‘San Andreas’, ‘Monterey’, and ‘Cabrillo’. This was confirmed in large-plot yield trials described below.
‘UCD Royal Royce’ was selected, with other test varieties, for advanced testing in large-plot yield trials (150 plants/plot) with five commercial growers in 2017-18. The selection criteria were: high cumulative marketable yields, high marketable fruit percentages, resistance to bruising and deterioration with harvest, handling, and storage, fruit appearance, and reduced runner production in coastal production environments. The production systems for large-plot yield trials were diverse and included: low-input organic (Santa Maria, Calif.), low-input fumigated (Salinas, Calif.), and high-input fumigated (Oso Flaco, Calif., Prunedale, Calif. and Moss Landing, Calif.). Fruit was harvested from February 2 to October 5 in Oso Flaco (52 harvests), April 3 to October 4 in Santa Maria (53 harvests), April 12 to October 6 in Prunedale (48 harvests), May 7 to October 8 in Moss Landing (45 harvests), and March 9 to October 12 in Salinas (36 harvests). The residual for statistical analyses was the entry×location interaction mean square.
The mean cumulative marketable yields ‘UCD Royal Royce’ was 12,456 cartons/acre=99,648 pounds/acre across production systems and locations (Table 3). Reduced runner production was observed for ‘UCD Royal Royce’ across trials. ‘UCD Royal Royce’ was the highest yielding cultivar through August, 2018 compared to ‘Monterey’, ‘Cabrillo’, and two other test cultivars (data not shown), and second highest yielding cultivar through October, 2018 compared to ‘Monterey’, ‘Cabrillo’ (Table 3) and two other test cultivars (data not shown). The cumulative marketable fruit yields of (Table 3) were significantly greater than ‘Monterey’ (p=0.039) and ‘Cabrillo’ (p=0.061) (Table 5). ‘UCD Royal Royce’ produced 1,820 cartons/acre (11,144 pounds/acre) more than ‘Cabrillo’ and 2,182 cartons/acre (17,464 pounds/acre) more than ‘Monterey’ (Tables 3 & 5). ‘UCD Royal Royce’ fruit weights were not significantly different from ‘Monterey’ (p=0.49) or ‘Cabrillo’ (p=0.46) (Tables 4 & 5).
To assess the quality of freshly harvested fruit, firmness (grams force), total soluble solids (SS) concentration, and titratable acid (TA) concentrations were measured from samples of fruit harvested on three dates from each location in 2017-18 (Tables 6-7). Harvest dates were one month apart with one replication per harvest date, 10 sub-samples per replication for firmness, and three subsamples per replication for SS and TA. Firmness was quantified with a hand-held penetrometer measuring the grams of force needed to puncture the fruit. SS and TA concentrations were quantified with benchtop instruments. The SS to TA ratio provides a relative measure of sweetness. To assess shelf-life, fruit weight (g/clamshell), SS, brightness (ordinal scale with 1=excellent to 5=unmarketable), liquid leakage (g/clamshell), and mold incidence (%) were quantified from samples of fruit harvested on two dates from each location with fruit stored under standard 4° C. conditions for 0, 7, 14, and 21 days (Table 8). Harvest dates were one month apart with one replication per harvest date.
‘UCD Royal Royce’ produced fruit meeting or exceeding industry standards for mass-production cultivars (Tables 6-8). The fruit was firm, withstood the rigors of harvest, packing, and storage, and maintained acceptable fruit quality and appearance for over two weeks of storage. ‘UCD Royal Royce’ produced significantly firmer fruit than either ‘Cabrillo’ (p<0.0001) or ‘Monterey’ (p<0.0001). ‘UCD Royal Royce’ additionally had significantly lower SS concentration than ‘Monterey’ (p<0.0001), but was not significantly different compared to ‘Cabrillo’ (p=0.78). The SS/TA ration for ‘UCD Royal Royce’ was significantly lower than ‘Monterey’ (p<0.0001) and ‘Cabrillo’ (p<0.019) .
‘UCD Royal Royce’ maintained adequate marketability and visual appeal over 14 days of post-harvest storage, the industry standard (Table 8), as did ‘Cabrillo’ and ‘Monterey’. The marketability of fruit stored for 21 days post-harvest was inadequate for all three cultivars. Fruit weight and brightness significantly decreased as post-harvest storage time increased (Table 8). The fruit weight decreases were not significantly different among cultivars. Cultivar×post-harvest storage time interactions were only statistically significant for liquid leakage and mold formation, with ‘Cabrillo’ deteriorating more than ‘UCD Royal Royce’ and ‘Monterey’ (Table 8).
‘UCD Royal Royce’ and additional cultivars were screened for resistance to Fusarium wilt, Verticillium wilt, Macrophomina, and Phytophthora crown rot in Davis, Calif. field experiments between 2015 and 2018. These included 2015-16 and 2016-17 Fusarium wilt screening experiments with 480 to 960 entries, a 2015-16 Macrophomina experiment with 960 entries, 2016-17 and 2017-18 Verticillium wilt experiments with 480 to 960 entries, and a 2017-18 Phytophthora crown rot experiment with 480 entries. Entries were arranged in randomized complete blocks experiment designs with four single-plant replications per entry. The 2015-16 experiments were planted in virgin soil in Davis, Calif. The 2016-17 and 2017-18 experiments were planted in fumigated soils in Davis, Calif. For each experiment, plants were artificially inoculated with the respective pathogen and phenotyped for disease symptoms on an ordinal scale, where 1=highly resistant (symptomless), 2=resistant, 3=intermediate, 4=susceptible, and 5=highly susceptible (dead). Within each experiment, plants were phenotyped at six different time points to study changes in the phenotypic distributions and quantify the progression of disease symptoms over time.
‘UCD Royal Royce’ was susceptible to Fusarium wilt (4.5 on scale), moderately susceptible to Verticillium wilt (3.1 on scale), moderately susceptible to Phytophthora crown rot (3.3 on scale), and susceptible to Macrophomina (5.0 on scale).
The following botanical descriptors are characteristic of ‘UCD Royal Royce’. The descriptors were collected from two different sites in May 2017 in Santa Maria, Calif. The characteristics of ‘UCD Royal Royce’ may vary in detail, depending upon environmental factors and culture conditions.
This application is a divisional application of U.S. patent application Ser. No. 15/932,994, filed Jun. 7, 2018.
| Number | Date | Country | |
|---|---|---|---|
| 20190380245 P1 | Dec 2019 | US |
| Number | Date | Country | |
|---|---|---|---|
| 61999632 | Jul 2014 | US | |
| 61997548 | Jun 2014 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15932994 | Jun 2018 | US |
| Child | 16501374 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15530135 | Dec 2016 | US |
| Child | 15932994 | US | |
| Parent | 14545653 | Jun 2015 | US |
| Child | 15530135 | US |
