Stress-regulated genes of plants, transgenic plants containing same, and methods of use

[0002] Three CD-R compact discs, labeled “Copy 1”, “Copy 2”, and “CRF” and having the files listed below, are submitted herewith and are incorporated herein by reference. Copy 1 and Copy 2 each contain two text documents: 1) a file named SCRIP1300-3_SEQUENCE_LISTING, which contains the Sequence Listing, was created on Aug. 20, 2001 (and recorded on the CD-R on Aug. 21, 2001), and is 9,972 KB in size; and 2) a file named SCRIP1300-3_Table—32, which contains Table 32, was created on Aug. 20, 2001 (and recorded on the CD-R on Aug. 21, 2001), and is 1,251 KB in size. The CRF contains a single file named SCRIP1300-3_SEQUENCE_LISTING, which contains the Sequence Listing, was created on Aug. 20, 2001 (and recorded on the CD-R on Aug. 21, 2001), is 9,972 KB in size, and is identical to the files having the same name on Copy 1 and Copy 2.

BACKGROUND OF THE INVENTION

[0003] 1. Field of the Invention

[0004] The present invention relates generally to plant genes, the expression of which are regulated in response to stress, and more specifically to the gene regulatory elements involved in a stress-induced response in plants, to uses of the coding sequences and regulatory elements of such plant stress-regulated genes, and to transgenic plants genetically modified to express such a coding sequence or to express a heterologous polynucleotide from such a regulatory element.

[0005] 2. Background Information

[0006] Microarray technology is a powerful tool that can be used to identify the presence and level of expression of a large number of polynucleotides in a single assay. A microarray is formed by linking a large number of discrete polynucleotide sequences, for example, a population of polynucleotides representative of a genome of an organism, to a solid support such as a microchip, glass slide, or the like, in a defined pattern. By contacting the microarray with a nucleic acid sample obtained from a cell of interest, and detecting those polynucleotides expressed in the cell can hybridize specifically to complementary sequences on the chip, the pattern formed by the hybridizing polynucleotides allows the identification of clusters of genes that are expressed in the cell. Furthermore, where each polynucleotide linked to the solid support is known, the identity of the hybridizing sequences from the nucleic acid sample can be identified.

[0007] A strength of microarray technology is that it allows the identification of differential gene expression simply by comparing patterns of hybridization. For example, by comparing the hybridization pattern of nucleic acid molecules obtained from cells of an individual suffering from a disease with the nucleic acids obtained from the corresponding cells of a healthy individual, genes that are differentially expressed can be identified. The identification of such differentially expressed genes provides a means to identify new genes, and can provide insight as to the etiology of a disease.

[0008] Microarray technology has been widely used to identify patterns of gene expression associated with particular stages of development or of disease conditions in animal model systems, and is being applied to the identification of specific patterns of gene expression in humans. The recent availability of information for the genomes of plants provides a means to adapt microarray technology to the study of plant gene expression.

[0009] Plants and plant products provide the primary sustenance, either directly or indirectly, for all animal life, including humans. For the majority of the world's human population and for many animals, plants and plant products provide the sole source of nutrition. As the world population increases, the best hope to prevent widespread famine is to increase the quantity and improve the quality of food crops, and to make the crops available to the regions of the world most in need of food.

[0010] Throughout history, a continual effort has been made to increase the yield and nutritious value of food crops. For centuries, plants having desirable characteristics such as greater resistance to drought conditions or increased size of fruit were crossbred and progeny plants exhibiting the desired characteristics were selected and used to produce seed or cuttings for propagation. Using such classical genetic methods, plants having, for example, greater disease resistance, increased yield, and better flavor have been obtained. The identification of plant genes involved in conferring a selective advantage on the plant to an environmental challenge would facilitate the generation and yield of plants, thereby increasing the available food supply to an increasing world population. The involvement of these genes in a single organism to responses to multiple stress conditions, however, remains unknown. Thus, a need exists to identify plant genes and polynucleotides that are involved in modulating the response of a plant to changing environmental conditions. The present invention satisfies this need and provides additional advantages.

SUMMARY OF THE INVENTION

[0011] The present invention relates to clusters of genes that are regulated in response to a stress condition in plants. Such clusters include, for example, plant polynucleotides whose expression is altered in response to two or more different stress conditions; and plant polynucleotides the expression of which are altered in response to one stress condition, but not to others. The identification of such clusters, using microarray technology, has allowed the identification of plant stress-regulated genes in Arabidopsis thaliana (see Tables 1 and 2); and homologs and orthologs thereof in other plant species (see Table 32). Thus, the invention provides isolated polynucleotide portions of Arabidopsis plant stress-regulated genes, and homologs and orthologs thereof; variants of such sequences, and polynucleotides encoding substantially similar plant stress-regulated polypeptides expressed therefrom. Such sequences include, for example, sequences encoding transcription factors; enzymes, including kinases; and structural proteins, including channel proteins (see Tables 29-31). Accordingly, the present invention also relates to an isolated polynucleotide comprising all or a portion of a plant stress-regulated gene, and to polynucleotide portions thereof, including a coding region (open reading frame), which encodes all or a portion of a stress-regulated polypeptide, for example, as set forth in SEQ ID NOS:1-2703; and a regulatory element involved in regulating the response of the plant to a stress condition such exposure to an abnormal level of salt, osmotic pressure, temperature or any combination thereof, for example, as set forth in SEQ ID NOS:2704-5379.

[0012] The present invention also relates to a recombinant polynucleotide, which contains a nucleotide sequence of a plant stress-regulated gene or functional portion thereof operatively linked to a heterologous nucleotide sequence. In one embodiment, the recombinant polynucleotide comprises a plant stress-regulated gene regulatory element operatively linked to a heterologous nucleotide sequence, which is not regulated by the regulatory element in a naturally occurring plant. The heterologous nucleotide sequence, when expressed from the regulatory element, can confer a desirable phenotype to a plant cell containing the recombinant polynucleotide. In another embodiment, the recombinant polynucleotide comprises a coding region, or portion thereof, of a plant stress-regulated gene operatively linked to a heterologous promoter. The heterologous promoter provides a means to express an encoded stress-regulated polypeptide constitutively, or in a tissue-specific or phase-specific manner.

[0013] Accordingly, in one aspect, the present invention provides an isolated polynucleotide comprising a nucleotide sequence of a plant gene that hybridizes under stringent conditions, preferably high stringency conditions, to any one of SEQ ID NOS:1-5379 (see Tables 1 and 2), including to a coding region (SEQ ID NOS:1-2703) or a regulatory region, which can alter transcription of an operatively linked nucleic acid sequence in response to an abiotic stress (SEQ ID NOS:2704-5379; see Table 2), or to a complement thereof. Additional aspects provide sequences that hybridize under stringent conditions, preferably high stringency conditions, to the complements of SEQ ID NO 1-1261 (cold responsive genes; Tables 3-6), SEQ ID NOS:2227-2427 (saline responsive genes; Tables 7-10), SEQ ID NOS:2428-2585 (osmotic responsive genes; Tables 11-14), SEQ ID NOS:1699-1969 (cold and osmotic responsive genes; Tables 15-17), SEQ ID NOS:1970-2226 (cold and saline responsive genes; Tables 18-20), SEQ ID NOS:2586-2703 (osmotic and saline responsive genes; Tables 21-23), and SEQ ID NOS:1262-1698(cold, osmotic and saline responsive genes; Tables 24-26), and which can comprise regulatory regions that can alter transcription in response to cold stress, osmotic stress, saline stress, or combinations thereof (SEQ ID NOS:2704-5379; see Table 2). Also provided are nucleotide sequences complementary thereto, and expression cassettes, plants and seeds comprising any of the above isolated sequences.

[0014] In another aspect, the present invention provides an isolated polynucleotide comprising a plant nucleotide sequence that hybridizes under stringent conditions, preferably high stringency conditions, to the complement of any one of SEQ ID NOS:1-2703 (Table 1), including to a coding region thereof (SEQ ID NOS:2704-5379), wherein expression of said coding region is altered in response to an abiotic stress. Additional aspects provide sequences that hybridize under high stringency conditions to the complements of SEQ ID NO 1-1261 (cold responsive genes; Tables 3-6), SEQ ID NOS:2227-2427 (saline responsive genes; Tables 7-10), SEQ ID NOS:2428-2585 (osmotic responsive genes; Tables 11-14), SEQ ID NOS:1699-1969 (cold and osmotic responsive genes; Tables 15-17), SEQ ID NOS:1970-2226 (cold and saline responsive genes; Tables 18-20), SEQ ID NOS:2586-2703 (osmotic and saline responsive genes; Tables 21-23), and SEQ ID NOS:1262-1698(cold, osmotic and saline responsive genes; Tables 24-26), and which can comprise a coding region whose transcription is altered in response to cold stress, osmotic stress, saline stress, or a combination thereof. Also provided are nucleotide sequences complementary thereto, and expression cassettes, plants and seeds comprising any of the above sequences.

[0015] The invention further relates to a method of producing a transgenic plant, which comprises at least one plant cell that exhibits altered responsiveness to a stress condition. In one embodiment, the method can be performed by introducing a polynucleotide portion of plant stress-regulated gene into a plant cell genome, whereby the polynucleotide portion of the plant stress-regulated gene modulates a response of the plant cell to a stress condition.

[0016] The polynucleotide portion of the plant stress-regulated gene can encode a stress-regulated polypeptide or functional peptide portion thereof (see SEQ ID NOS:1-2703), wherein expression of the stress-regulated polypeptide or functional peptide portion thereof either increases the stress tolerance of the transgenic plant, or decreases the stress tolerance of the transgenic plant. The polynucleotide portion of the plant stress-regulated gene encoding the stress-regulated polypeptide or functional peptide portion thereof can be operatively linked to a heterologous promoter. The polynucleotide portion of the plant stress-regulated gene also can comprise a stress-regulated gene regulatory element (see SEQ ID NOS:2704-5379). The stress-regulated gene regulatory element can integrate into the plant cell genome in a site-specific manner, whereupon it can be operatively linked to a heterologous nucleotide sequence, which can be expressed in response to a stress condition specific for the regulatory element; or can be a mutant regulatory element, which is not responsive to the stress condition, whereby upon integrating into the plant cell genome, the mutant regulatory element disrupts an endogenous stress-regulated regulatory element of a plant stress-regulated gene, thereby altering the responsiveness of the plant stress-regulated gene to the stress condition.

[0017] In one aspect, the invention provides a method for producing a transgenic plant by introducing into at least one plant cell a recombinant nucleic acid construct comprising i) all or a portion of any one of SEQ ID NOS:1-5379; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to all or a portion of the complement of any one of SEQ ID NOS:1-2703; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to abiotic stress, and that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2704-5379; iv) a polynucleotide having at least 90% sequence identity with any one of SEQ ID NO:1-5379; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a nucleotide sequence that alters transcription of an operatively linked coding region in response to abiotic stress; and regenerating a plant from the at least one plant cell.

[0018] Another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:1-1261 or 2704-3955; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:1-1261; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to cold stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2704-3955; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:1-1261 or 2704-3955; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv) wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to cold stress; and regenerating a plant from the at least one plant cell.

[0019] In another aspect, the invention provides a method for producing a transgenic plant by introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:2428-2585 or 5108-5263; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2428-2585; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to osmotic stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:5108-5263; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:2428-2585 or 5108-5263; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to osmotic stress; and regenerating a plant from the at least one plant cell.

[0020] Still another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:2227-2427 or 4910-5107; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2227-2427; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to saline stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2227-2427; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:4910-5107; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv) wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to saline stress; and regenerating a plant from the at least one plant cell.

[0021] Yet another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:1699-1969 or 4389-4654; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:1699-1969; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to a combination of cold and osmotic stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:4389-4654; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:1699-1969 or 4389-4654; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to a combination of cold and osmotic stress; and regenerating a plant from the at least one plant cell.

[0022] Yet another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:1970-2226 or 4655-4909; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:1970-2226; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to a combination of cold and saline stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:4655-4909; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:1970-2226 or 4655-4909; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to a combination of cold and saline stress; and regenerating a plant from the at least one plant cell.

[0023] A further aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:2586-2703 or 5264-5379; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2586-2703; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to a combination of osmotic and saline stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS: 5264-5379; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:2586-2703 or 5264-5379; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to a combination of osmotic and saline stress; and regenerating a plant from the at least one plant cell.

[0024] Another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:1262-1698 or 3956-4388; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:1262-1698; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to a combination of cold, osmotic and saline stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:3956-4388; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:1262-1698 or 3956-4388; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv) wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to a combination of cold, osmotic and saline stress; and regenerating a plant from the at least one plant cell. Further aspects include plants and uniform populations of plants made by the above methods as well as seeds and progeny from such plants.

[0025] In another embodiment, a transgene introduced into a plant cell according to a method of the invention can encode a polypeptide that regulates expression from an endogenous plant stress-regulated gene. Such a polypeptide can be, for example, a recombinantly produced polypeptide comprising a zinc finger domain, which is specific for the regulatory element, and an effector domain, which can be a repressor domain or an activator domain. The polynucleotide encoding the recombinant polypeptide can be operatively linked to and expressed from a constitutively active, inducible or tissue specific or phase specific regulatory element. Expression of the recombinant polypeptide from a plant stress-regulated promoter as disclosed herein can be particularly advantageous in that the polypeptide can be coordinately expressed with the endogenous plant stress-regulated genes upon exposure to a stress condition. The invention also provides transgenic plants produced by a method as disclosed, as well as to a plant cell obtained from such transgenic plant, wherein said plant cell exhibits altered responsiveness to the stress condition; a seed produced by the transgenic plant; and a cDNA or genomic DNA library prepared from the transgenic plant, or from a plant cell from said transgenic plant, wherein said plant cell exhibits altered responsiveness to the stress condition.

[0026] In one aspect, the invention provides an isolated nucleic acid molecule comprising a nucleotide sequence substantially similar to a sequence of any one of SEQ ID NOS:2704-5379, which can alter transcription of an operatively linked polynucleotide in a plant cell in response to an abiotic stress. Additional aspects of the invention provide isolated polynucleotides, including, for example, sequences substantially similar to any of SEQ ID NOS:2704-3955, which can alter transcription of an operatively linked polynucleotide in response to a cold stress; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:5108-5263, which can alter transcription of an operatively linked polynucleotide in response to an osmotic stress; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:4910-5107, which can alter transcription of an operatively linked polynucleotide in response to a saline stress; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:4389-4654, which can alter transcription of an operatively linked polynucleotide in response to a combination of cold and osmotic stresses; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:4655-4909, which can alter transcription of an operatively linked polynucleotide in response to a combination of cold and saline stresses; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:5264-5379, which can alter transcription of an operatively linked polynucleotide in response to a combination of osmotic and saline stresses; and isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:3956-4388, which can alter transcription of an operatively linked polynucleotide in response to a combination of cold, osmotic and saline stresses.

[0027] Related aspects of the invention provide an isolated nucleotide sequences that can alter transcription of an operatively linked polynucleotide in response to an abiotic stress, and that hybridize under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:2704-5379. Additional aspects provide an isolated nucleotide sequence that can alter transcription of an operatively linked polynucleotide in response to cold stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:2704-3955; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to osmotic stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:5108-5263; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to saline stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:4910-5107; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to a combination of cold and osmotic stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:4389-4654; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to a combination of cold and saline stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:4655-4909; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to an combination of osmotic and saline stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:5264-5379; and a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to a combination of cold, osmotic and saline stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:3956-4388.

[0028] Further aspects provide an expression cassette comprising as operatively linked components any of the above isolated nucleic acid sequences that alter transcription, a coding region, and a termination sequence. Also provided are host cells and seeds comprising such expression cassettes, plants containing such host cells and seeds and progeny of plants containing said host cells. In related aspects, the coding region of the expression cassettes comprise sequences encoding marker proteins and sequences involved in gene silencing such as antisense sequences, double stranded RNAi sequences, a triplexing agent, and sequences comprising dominant negative mutations. In additional related aspects, the coding regions comprise sequences encoding polypeptides that alter the response of a plant to an abiotic stress.

[0029] The present invention also relates to a method of modulating the responsiveness of a plant cell to a stress condition. Such a method can be performed, for example, by introducing a polynucleotide portion of a plant stress-regulated genes described herein into the plant cell, thereby modulating the responsiveness of the plant cell to a stress condition. Such a method can result in the responsiveness of the plant cell being increased upon exposure to the stress condition, which, in turn, can result in increased or decreased tolerance of the plant cell to a stress condition; or can result in the responsiveness of the plant cell to the stress condition being decreased, which, in turn, can result in increased or decreased tolerance of the plant cell to a stress condition. In one embodiment, the polynucleotide portion of the plant stress-regulated gene can integrate into the genome of the plant cell, thereby modulating the responsiveness of the plant cell to the stress condition. In another embodiment, the polynucleotide portion of the plant stress-regulated gene encodes a stress-regulated polypeptide or functional peptide portion thereof, and can be operatively linked to a heterologous promoter. The polynucleotide portion of the plant stress-regulated gene also can contain a mutation, whereby upon integrating into the plant cell genome, the polynucleotide disrupts (knocks-out) an endogenous plant stress-regulated sequence, thereby modulating the responsiveness of the plant cell to the stress condition. Depending on whether the knocked-out gene encodes an adaptive or a maladaptive stress-regulated polypeptide, the responsiveness of the plant will be modulated accordingly.

[0030] The present invention further relates to a method of modulating the activity of a biological pathway in a plant cell, wherein the pathway involves a stress-regulated polypeptide or a non-protein regulatory molecule. Such a method can be performed by introducing a polynucleotide portion of a plant stress-regulated gene, or a polynucleotide derived therefrom, for example a ribozyme derived from a nucleotide sequence as set forth in any of SEQ ID NOS:1-2703, into the plant cell, thereby modulating the activity of the biological pathway. The method can be performed with respect to a pathway involving any of the stress-regulated polypeptides as disclosed herein or encoded by the polynucleotides disclosed herein, as well as using homologs or orthologs thereof. In one embodiment, the method is performed by introducing a polynucleotide portion of a plant stress-regulated gene into the plant cell, wherein the plant stress-regulated gene comprises a nucleotide sequence as set forth in any of SEQ ID NOS:1-155, 157-228, 230-232, 234-557, 559-572, 574-605, 607-634, 636-786, 788-812, 814-1262, 1264-1386, 1387-1390, 1392-1404, 1406-1444, 1446-1483, 1485-1588, 1590-1608, 1610-1633, 1634-1725, 1727-1865, 1867-1917, 1919-1927, 1929-2855, 2857-2928, 2930-2932, 2934-3256, 3258-3271, 3273-3304, 3306-3323, 3325-3333, 3335-3485, 3487-3511, 3313-3956, 3958-4078, 4080-4097, 4099-4136, 4138-4175, 4177-4279, 4281-4299, 4301-4324, 4326-4414, 4416-4552, 4554-4602, and 4604-5379, thereby modulating the activity of the biological pathway.

[0031] The present invention also relates to a method of identifying a polynucleotide that modulates a stress response in a plant cell. In one embodiment the method comprises determining gene expression in a plant exposed to at least one stress to produce an expression profile and identifying sequences whose expression is altered at least two fold compared to plants not exposed to the stress. Such an expression profile can be obtained, for example, by contacting an array of probes representative of a plant cell genome with nucleic acid molecules expressed in a plant cell exposed to the stress; and detecting one or more nucleic acid molecules expressed at a level different from a level of expression in the absence of the stress. The method can further comprise introducing the differentially expressed nucleic acid molecule into a plant cell; and detecting a modulated response of the genetically modified plant cell to a stress, thereby identifying a polynucleotide that modulates a stress response in a plant cell. The stress can be any stress, for example, an abiotic stress such as exposure to an abnormal level of cold, osmotic pressure, and salinity. The contacting is under conditions that allow for selective hybridization of a nucleic acid molecule with probe having sufficient complementarity, for example, under stringent hybridization conditions. Expression of the nucleic acid molecule can increase or decrease the tolerance of the plant cell to the stress, and the nucleic acid molecule can be expressed at a level that is less than or greater than the level of expression in the absence of the stress.

[0032] In still another embodiment, the polynucleotide portion of the plant stress-regulated gene can comprise a stress-regulated regulatory element, which can be operatively linked to a heterologous nucleotide sequence, the expression of which can modulate the responsiveness of the plant cell to a stress condition. Such a heterologous nucleotide sequence can encode, for example, a stress-inducible transcription factor such as DREB1A. The heterologous nucleotide sequence also can encode a polynucleotide that is specific for a plant stress-regulated gene, for example, an antisense molecule, an RNAi molecule, a ribozyme, and a triplexing agent, any of which, upon expression in the plant cell, reduces or inhibits expression of a stress-regulated polypeptide encoded by the gene, thereby modulating the responsiveness of the plant cell to a stress condition, for example, an abnormal level of cold, osmotic pressure, and salinity. In another aspect, the method can include introducing a polynucleotide portion of a plant stress-regulated gene into the plant cell, wherein the plant stress-regulated gene includes a nucleotide sequence of a polynucleotide as set forth in any of SEQ ID NOS:1-155, 157-228, 230-232, 234-557, 559-572, 574-605, 607-634, 636-786, 788-812, 814-1262, 1264-1386, 1387-1390, 1392-1404, 1406-1444, 1446-1483, 1485-1588, 1590-1608, 1610-1633, 1634-1725, 1727-1865, 1867-1917, 1919-1927, 1929-2855, 2857-2928, 2930-2932, 2934-3256, 3258-3271, 3273-3304, 3306-3323, 3325-3333, 3335-3485, 3487-3511, 3313-3956, 3958-4078, 4080-4097, 4099-4136, 4138-4175, 4177-4279, 4281-4299, 4301-4324, 4326-4414, 4416-4552, 4554-4602, and 4604-5379, thereby modulating the responsiveness of the plant cell to a stress condition. The invention also relates to a plant cell obtained by any of the methods of modulating the responsiveness of a plant to a stress condition or combination of stress conditions, and to a plant comprising such a plant cell.

[0033] The present invention further relates to a method of selecting a plant having an altered resistance to an abiotic stress condition or a combination of abiotic stress conditions, such a method being useful for marker-assisted breeding. Such a method can be performed, for example, by contacting nucleic acid molecules representative of expressed polynucleotides in a plant cell of a plant to be examined for having an altered resistance to an abiotic stress with a nucleic acid probes that selectively hybridizes under stringent conditions to a plant stress-regulated gene comprising a nucleotide sequence as set forth in any of SEQ ID NO:1-5379; detecting a level of selective hybridization of the nucleic acid probes to a nucleic acid molecule representative of an expressed polynucleotide in the plant cell, wherein the level of selective hybridization corresponds to the level of the expressed polynucleotide in the plant cell, which is indicative of resistance of the plant to an abiotic stress; and selecting a plant having a level of expression of a polynucleotide indicative of altered resistance to an abiotic stress condition. For example, the abiotic stress condition can be cold stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1-1261 and 2704-3955, for example, at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1-155, 157-228, 230-232, 234-557, 559-572, 574-605, 607-634, 636-786, 788-812, 814-1261, 2704-2855, 2857-2928, 2930-2932, 2934-3256, 3258-3271, 3273-3304, 3306-3323, 3325-3333, 3335-3485, 3487-3511, and 3313-3955; or the abiotic stress condition can be saline stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2226-2427 and 4910-5107; or the abiotic stress condition can be osmotic stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2428-2585 and 5108-5263. In addition, a combination of abiotic stress conditions can be a combination of cold stress and osmotic stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1669-1969 and 4389-4654, for example, at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1699-1725, 1727-1865, 1867-1917, 1919-1927, 1929-1969, 4389-4414, 4416-4552, 4554-4602, 4604-4612, and 4613-4654; or the combination of abiotic stress conditions can be a combination of cold stress and saline stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS: 1970-2226 and 4655-4909; or the combination of abiotic stress conditions can be a combination of osmotic stress and saline stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2586-2703 and 5264-5379; or the combination of abiotic stress conditions can be a combination of cold stress, osmotic stress and saline stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1262-1698 and 3956-4388, for example, at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1262, 1264-1386, 1387-1390, 1392-1404, 1406-1444, 1446-1483, 1485-1588, 1590-1608, 1610-1633, 1634-1698, 3956, 3958-4078, 4080-4097, 4099-4136, 4138-4175, 4177-4279, 4281-4299, 4301-4324, and 4326-4388.

[0034] The present invention also relates to a method of expressing a heterologous nucleotide sequence in a plant cell. Such a method can be performed, for example, by introducing into the plant cell a plant stress-regulated regulatory element operatively linked to the heterologous nucleotide sequence, whereby, upon exposure of the plant cell to a stress condition, the heterologous nucleotide sequence is expressed in the plant cell. In one embodiment, the stress-regulated gene regulatory element is any of the sequences described herein that are capable of altering transcription of an operatively linked sequence in response to an abiotic stress, for example, SEQ ID NOS:2704-5379. In another embodiment, stress-regulated gene regulatory element comprises a nucleotide sequence as set forth in any of SEQ ID NOS:2704-2855, 2857-2928, 2930-2932, 2934-3256, 3258-3271, 3273-3304, 3306-3323, 3325-3333, 3335-3485, 3487-3511, 3313-3956, 3958-4078, 4080-4097, 4099-4136, 4138-4175, 4177-4279, 4281-4299, 4301-4324, 4326-4414, 4416-4552, 4554-4602, and 4604-5379, whereby, upon exposure of the plant cell to stress condition, the heterologous nucleotide sequence is expressed in the plant cell. The heterologous nucleotide sequence can encode a selectable marker, a diagnostic marker, or a polypeptide that confers a desirable trait upon the plant cell, for example, a polypeptide that improves the nutritional value, digestibility or ornamental value of the plant cell, or a plant comprising the plant cell.

[0035] The present invention additionally relates to a method of identifying a stress condition to which a plant cell was exposed by comparing an expression profile from a test plant suspected of having been exposed to at least one stress condition to an expression profile obtained from a reference plant, preferably of the same species, which has been exposed to the suspected stress condition. Such a method can be performed, for example, by contacting nucleic acid molecules representative of expressed polynucleotides in cells of the test plant with at least one nucleic acid probe under conditions suitable for selective hybridization to a complementary nucleotide sequence, wherein the probe comprises at least 15 nucleotides of a plant stress-regulated gene, wherein the stress-regulated gene does not have a nucleotide sequence of a polynucleotide as set forth in any of SEQ ID NOS:156, 229, 233, 558, 573, 606, 635, 787, 813, 1263, 1386, 1391, 1405, 1445, 1484, 1589, 1609, 1634, 1726, 1866, 1918 or 1928, or a nucleotide sequence complementary thereto, whereby detecting selective hybridization of at least one nucleic acid probe, or detecting a change in a level of selective hybridization as compared to a level of selective hybridization obtained using nucleic acid molecules representative of expressed polynucleotides in cells of a plant known not have been exposed to an abiotic stress, indicates that the test plant has been exposed to an abiotic stress, and whereby an absence of selective hybridization of at least one nucleic acid probe indicates that the test plant has not been exposed to an abiotic stress. For example, the abiotic stress is cold stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1-155, 157-228, 230-232, 234-557, 559-572, 574-605, 607-634, 636-786, 788-812, 814-1261 or a nucleotide sequence complementary thereto; or the abiotic stress can be a saline stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2226-2427 or a nucleotide sequence complementary thereto; or the abiotic stress can be an osmotic stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in two or more of SEQ ID NOS:2428-2585 or a nucleotide sequence complementary thereto.

[0036] A method of identifying a stress condition to which a plant cell was exposed also can be performed, for example, by contacting nucleic acid molecules expressed in the test plant cell with an array of probes representative of the plant cell genome; detecting a profile of expressed nucleic acid molecules characteristic of a stress response, and comparing the expression pattern in the test plant to the expression pattern obtained from a reference plant thereby identifying the stress condition to which the plant cell was exposed. The contacting is under conditions that allow for selective hybridization of a nucleic acid molecule with probes having sufficient complementarity, for example, under stringent hybridization conditions. The profile can be characteristic of exposure to a single stress condition, for example, an abnormal level of cold, osmotic pressure, or salinity, or can be characteristic of exposure to more than one stress condition, for example, cold, increased osmotic pressure and increased salinity. In one embodiment, the nucleotide sequence of a gene whose expression is detected is selected from a polynucleotide comprising any of SEQ ID NOS:1-2703. In further embodiments, the nucleotide sequence of a gene that is expressed in response a particular stress or combination of stresses can comprise a polynucleotide expressed in response to cold stress (SEQ ID NOS:1-1261), osmotic stress (SEQ ID NOS:2428-2585), saline (salt) stress (SEQ ID NOS:2227-2427), a combination of cold and osmotic stress (SEQ ID NOS:1699-1969), a combination of saline and osmotic stress (SEQ ID NOS:1970-2226), a combination of osmotic and saline stress (SEQ ID NOS:2586-2703), or a combination of cold, osmotic and saline stress (SEQ ID NOS:1262-1698).

[0037] In another embodiment, the method can be used for determining whether a test plant has been exposed to a combination of abiotic stress conditions. Such a method can be performed, for example, by contacting nucleic acid molecules representative of expressed polynucleotides in cells of the test plant with at least one nucleic acid probe under conditions suitable for selective hybridization to a complementary nucleotide sequence, whereby detecting selective hybridization of at least one nucleic acid probe, or detecting a change in a level of selective hybridization as compared to a level of selective hybridization obtained using nucleic acid molecules representative of expressed polynucleotides in cells of a plant known not have been exposed to a combination of stress conditions, indicates that the test plant has been exposed to a combination of abiotic stress conditions, and whereby an absence of selective hybridization of at least one nucleic acid probe indicates that the test plant has not been exposed to a combination of abiotic stress conditions. For example, the combination of abiotic stress conditions can be a combination of a cold stress and an osmotic stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1699-1969, or a nucleotide sequence complementary thereto; or the combination of abiotic stress conditions can be a combination of a cold stress and a saline stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1970-2226, or a nucleotide sequence complementary thereto; or the combination of abiotic stress conditions can be a combination of an osmotic stress and a saline stress, and the probe can included at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2586-2703, or a nucleotide sequence complementary thereto; or the combination of abiotic stress conditions can be a combination of a cold stress, a saline stress and an osmotic stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1262-1698, or a nucleotide sequence complementary thereto.

[0038] The present invention also relates to a method for monitoring a population of plants for exposure to a stress condition or combination of stress conditions. Such a method can be performed, for example, by introducing into the population of a plants a sentinel plant, wherein said sentinel plant is a transgenic plant, which contains plant cells containing a stress-regulated regulatory element operatively linked to a polynucleotide encoding a detectable marker; and examining the sentinel plant for expression of the detectable marker, which is indicative of exposure of the population of plants to a stress condition or combination of stress conditions. The stress condition or combination of stress conditions can be any such condition or conditions, particularly an abiotic stress condition or combination of abiotic stress conditions. The detectable marker can be any reporter molecule that is readily or conveniently detectable, particularly a marker that is visibly detectable, for example, a luminescent detectable marker such as luciferin, or a fluorescent detectable marker such as a green fluorescent protein, a yellow fluorescent protein, a cyan fluorescent protein, a red fluorescent protein, or an enhanced or modified form thereof.

[0039] The present invention further relates to a transgenic plant, which contains a nucleic acid construct comprising a polynucleotide portion of plant stress-regulated polynucleotide. In one embodiment, the transgenic plant exhibits altered responsiveness to a stress condition as compared to a corresponding reference plant not containing the construct. Such a transgenic plant can contain, for example, a construct that disrupts an endogenous stress-regulated gene in the plant, thereby reducing or inhibiting expression of the gene in response to a stress condition. Such a knock-out can increase or decrease tolerance of the plant to a stress condition. The transgene also can comprise a coding sequence of a plant stress-regulated gene, which can be operatively linked to a heterologous regulatory element such as a constitutively active regulatory element, an regulated regulatory element, a tissues specific or phase specific regulatory element, or the like. In another embodiment, the transgenic plant contains a nucleic acid construct comprising a plant stress-regulated regulatory element, which can be operatively linked to a heterologous nucleotide sequence that can encode a polypeptide. Expression of the heterologous polypeptide can confer a desirable characteristic on the plant, for example, can improve the nutritional or ornamental value of the transgenic plant. In still another embodiment, the transgenic plant contains multiple nucleic acid constructs, which can be multiple copies of the same construct, or can be two or more different constructs.

[0040] The present invention also relates to a plant stress-regulated regulatory element, which is obtained from a plant stress-regulated polynucleotide disclosed herein for example any of SEQ ID NOS:2704-5379; a homolog or ortholog thereof The invention also provides a method of identifying an agent, for example a transcription factor, that specifically binds to or activates a plant stress-regulated regulatory element. Such a method can be performed, for example, by contacting the regulatory element with a plant cell extract, and identifying polypeptides that specifically bind to the regulatory element. Confirmation that the specifically binding polypeptide is a transcription factor can be demonstrated using, for example, the stress-regulated regulatory element operably linked to a reporter gene, and detecting expression of the reporter gene. Control constructs comprising a regulatory element, other than a plant stress-regulated regulatory element, operatively linked to a reporter molecule can be used to confirm that the transcription factor is specific for the plant stress-regulated regulatory element. A polynucleotide encoding such a transcription factor also can be obtained.

[0041] The present invention also relates to a method of using a polynucleotide portion of a plant stress-regulated gene to confer a selective advantage on a plant cell. In one embodiment, such a method is performed by introducing a plant stress-regulated regulatory element into a plant cell such as those described herein, wherein, upon exposure of the plant cell to a stress condition to which the regulatory element is responsive, a nucleotide sequence operatively linked to the regulatory element is expressed, thereby conferring a selective advantage to plant cell. The operatively linked nucleotide sequence can be, for example, a transcription factor, the expression of which induces the further expression of polynucleotides involved in a stress response, thereby enhancing the response of a plant to the stress condition. In another embodiment, a coding sequence of a plant stress-regulated gene as disclosed herein is introduced into the cell, thereby providing the plant with a selective advantage in response to a stress condition. In still another embodiment, the method results in the knock-out of a plant stress-regulated gene as disclosed herein in a first population of plants, thereby providing a selective advantage to a stress condition in a second population of plants.

[0042] The invention further relates to a method of identifying an agent that modulates the activity of a stress-regulated regulatory element of a plant. In a particular embodiment, is provided a method for identifying an agent that alters the activity of an abiotic stress responsive regulatory element comprising contacting the agent or a composition containing an agent to be tested with at least one abiotic stress responsive regulatory element, preferably selected from the group consisting of SEQ ID NOS:2704-5379 (see Table 2), and determining the effect of the agent on the ability of the regulatory sequence to regulate transcription. In further embodiments, the regulatory elements are associated with particular stresses or combination of stresses such as cold stress (SEQ ID NOS:2704-3955), osmotic stress (SEQ ID NOS:5108-5263), saline stress (SEQ ID NOS:4910-5107), a combination of cold and osmotic stress (SEQ ID NOS:4389-4654), a combination of cold and saline stress (SEQ ID NOS:4655-4909), a combination of osmotic and saline stress (SEQ ID NOS:5264-5379), or a combination of cold, osmotic and saline stress (SEQ ID NOS:3956-4388). In one embodiment, the regulatory element can be operatively linked to a heterologous polynucleotide encoding a reporter molecule, and an agent that modulates the activity of the stress-regulated regulatory element can be identified by detecting a change in expression of the reporter molecule due to contacting the regulatory element with the agent. Such a method can be performed in vitro in a plant cell-free system, or in a plant cell in culture or in a plant in situ. In another embodiment, the agent is contacted with a transgenic plant containing an introduced plant stress-regulated regulatory element, and an agent that modulates the activity of the regulatory element is identified by detecting a phenotypic change in the transgenic plant. The methods of the invention can be performed in the presence or absence of the stress condition to which the particularly regulatory element is responsive.

[0043] Another aspect provides a method for identifying an agent that alters abiotic stress responsive polynucleotide expression in a plant or plant cell comprising contacting a plant or plant cell with a test agent; subjecting the plant cell or plant cell to an abiotic stress or combination of stresses before, during or after contact with the agent to be tested; obtaining an expression profile of the plant or plant cell and comparing the expression profile of the plant or plant cell to an expression profile from a plant or plant cell not exposed to the abiotic stress or combination of stresses. In one embodiment, the expression profile comprises expression data for at least one nucleotide sequence comprising any of SEQ ID NOS:1-5379 (see Tables 1 and 2). In additional embodiments, the expression profile comprises expression data for at least one, and preferably two or more sequences associated with a particular abiotic stress or combination of stresses such as cold stress (SEQ ID NOS:1-1261 and 2704-3955), osmotic stress (SEQ ID NOS:2428-2585 and 5108-5263), saline stress (SEQ ID NOS:2227-2427 and 4910-5107), a combination of cold and osmotic stress (SEQ ID NOS:1699-1969 and 4389-4654), a combination of cold and saline stress (SEQ ID NOS:1970-2226 and 4655-4909), a combination of osmotic and saline stress (SEQ ID NOS:2586-2703 and 5264-5379), or a combination of cold, osmotic and saline stress (SEQ ID NOS:1262-1698 and 3956-4388).

[0044] Still another aspect provides nucleotide probes useful for detecting an abiotic stress response in plants, the probes comprising a nucleotide sequence of at least 15, 25, 50 or 100 nucleotides that hybridizes under stringent, preferably highly stringent, conditions to at least one sequence comprising any of SEQ ID NOS:1-2703. Also provided are nucleotide probes comprising at least 15, 25, 50 or 100 nucleotides in length that hybridize under stringent, preferably highly stringent conditions, to at least one gene associated with a particular stress or combination of stresses, for example cold stress, (SEQ ID NOS:1-1261), osmotic stress (SEQ ID NOS:2428-2585), saline stress (SEQ ID NOS:2227-2427), a combination of cold and osmotic stress (SEQ ID NOS:1699-1969), a combination of cold and saline stress (SEQ ID NOS: 1970-2226), a combination of osmotic and saline stress (SEQ ID NOS:2586-2703), or a combination of cold, osmotic, and saline stress (SEQ ID NOS:1262-1698).

[0045] An additional aspect provides a method for marker-assisted breeding to select plants having an altered resistance to abiotic stress comprising obtaining nucleic acid molecules from the plants to be selected; contacting the nucleic acid molecules with one or more probes that selectively hybridize under stringent, preferably highly stringent, conditions to a nucleic acid sequence selected from the group consisting of SEQ ID NOS:1-2703; detecting the hybridization of the one or more probes to the nucleic acid sequences wherein the presence of the hybridization indicates the presence of a gene associated with altered resistance to abiotic stress; and selecting plants on the basis of the presence or absence of such hybridization. Marker-assisted selection can also be accomplished using one or more probes which selectively hybridize under stringent, preferably highly stringent conditions, to a nucleotide sequence comprising a polynucleotide expressed in response associated with a particular stress, for example, a nucleotide sequence comprising any of SEQ ID NOS:1-1261 (cold stress), SEQ ID NOS:2428-2585 (osmotic stress), SEQ ID NOS:2227-2427 (saline stress), SEQ ID NOS:1699-1969 (cold and osmotic stress), SEQ ID NOS:1970-2226 (cold and saline stress), SEQ ID NOS:2586-2703 (osmotic and saline stress), or SEQ ID NOS:1262-1698 (cold, osmotic and saline stress). In each case marker-assisted selection can be accomplished using a probe or probes to a single sequence or multiple sequences. If multiple sequences are used they can be used simultaneously or sequentially.

[0046] A further aspect provides a method for monitoring a population of plants comprising providing at least one sentinel plant containing a recombinant polynucleotide comprising a stress responsive regulatory sequence selected from the group consisting of SEQ ID NOS:2704-5379 which is operatively linked to a nucleotide sequence encoding a detectable marker, for example a fluorescent protein. Additional aspects provide the use of various regulatory sequences including those associated with cold stress (SEQ ID NOS:2704-3955), osmotic stress (SEQ ID NOS:5108-5263), saline stress (SEQ ID NOS:4910-5107), cold and osmotic stress (SEQ ID NOS:4389-4654), cold and saline stress (SEQ ID NOS:4655-4909), osmotic and saline stress (SEQ ID NOS:5264-5379), and cold, osmotic and saline stress (SEQ ID NOS:3956-4388), or fragments thereof wherein such fragments can alter transcription of an operatively linked nucleotide sequence in response to an abiotic stress.

[0047] A further aspect provides a computer readable medium having stored thereon computer executable instructions for performing a method comprising receiving data on gene expression in a test plant of at least one nucleic acid molecule having at least 70%, preferably at least 80%, more preferably at least 90%, and most preferably at least 95% nucleotide sequence identity to one or more polynucleotide sequences as set forth in any of SEQ ID NOS:1-2703; and comparing expression data from the test plant to expression data for the same polynucleotide sequence or sequences in a plant that has been exposed to at least one abiotic stress.

[0048] Yet a further aspect provides a computer readable medium having stored thereon a data structure comprising, sequence data for at least one, and preferably a plurality of nucleic acid molecules having at least 70%, preferably at least 80%, more preferably at least 90%, and most preferably at least 95% nucleotide sequence identity to a polynucleotide comprising any of SEQ ID NOS:1-2703, or the complement thereof; and a module receiving the nucleic acid molecule sequence data which compares the nucleic acid molecule sequence data to at least one other nucleic acid sequence.

DETAILED DESCRIPTION OF THE INVENTION

[0049] The present invention relates to clusters of genes that are induced in response to one or a combination of abiotic stress conditions. Abiotic stress conditions, such as a shortage or excess of solar energy, water and nutrients, and salinity, high and low temperature, or pollution (e.g., heavy metals), can have a major impact on plant growth and can significantly reduce the yield, for example, of cultivars. Under conditions of abiotic stress, the growth of plant cells is inhibited by arresting the cell cycle in late G1, before DNA synthesis, or at the G2/M boundary (see Dudits, Plant Cell Division, Portland Press Research, Monograph; Francis, Dudits, and Inze, eds., 1997; chap. 2, page 21; Bergounioux, Protoplasma 142:127-136, 1988). The identification of stress-regulated gene clusters, using microarray technology, provides a means to identify plant stress-regulated genes.

[0050] As used herein, the term “cluster,” when used in reference to stress-regulated genes, refers to nucleotide sequences of genes that have been selected by drawing Venn diagrams, and selecting those genes that are regulated only by a selected stress condition. In general, a cluster of stress-regulated genes includes at least 5, 10, 15, or 20 genes, including polynucleotide portions thereof, each of which is responsive to the same selected stress condition or conditions. The selected stress condition can be a single stress condition, for example, cold, osmotic stress or salinity stress (see Tables 3-14), or can be a selected combination of stress conditions, for example, cold, osmotic stress and salinity stress (see Tables 15-26). In addition, a cluster can be selected based on specifying that all of the genes are coordinately regulated, for example, they all start at a low level and are induced to a higher level. However, a cluster of saline stress-regulated genes, for example, that was selected for coordinate regulation from low to high, also can be decreased in response to cold or mannitol. By varying the parameters used for selecting a cluster of gene nucleotide sequences, those genes that are expressed in a specific manner following a stress can be identified.

[0051] As used herein in reference to a polynucleotide or polynucleotide portion of a gene or nucleic acid molecule, the term “isolated” means a polynucleotide, polynucleotide portion of a gene, or nucleic acid molecule that is free of one or both of the nucleotide sequences that normally flank the polynucleotide in a genome of a naturally-occurring organism from which the polynucleotide is derived. The term includes, for example, a polynucleotide or fragment thereof that is incorporated into a vector or expression cassette; into an autonomously replicating plasmid or virus; into the genomic DNA of a prokaryote or eukaryote; or that exists as a separate molecule independent of other polynucleotides. It also includes a recombinant polynucleotide that is part of a hybrid polynucleotide, for example, one encoding a polypeptide sequence.

[0052] The terms “polynucleotide,” “oligonucleotide,” and “nucleic acid sequence” are used interchangeably herein to refer to a polymeric (2 or more monomers) form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. Although nucleotides are usually joined by phosphodiester linkages, the term also includes polymers containing neutral amide backbone linkages composed of aminoethyl glycine units. The terms are used only to refer to the primary structure of the molecule. Thus, the term includes double stranded and single stranded DNA molecules, including a sense strand or an antisense strand, and RNA molecules as well as genomic DNA, cDNA, mRNA and the like. It will be recognized that such polynucleotides can be modified, for example, by including a label such as a radioactive, fluorescent or other tag, by methylation, by the inclusion of a cap structure, by containing a substitution of one or more of the naturally occurring nucleotides with a nucleotide analog, by containing an internucleotide modification such as having uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoramidates, carbamates, or the like), by containing a pendant moiety such as a protein (e.g., a nuclease, toxin, antibody, signal peptide, poly-L-lysine, or the like), by containing an intercalator such as acridine or psoralen, by containing a chelator, which can be a metal such as boron, an oxidative metal, or a radioactive metal, by containing an alkylator, or by having a modified linkage (e.g., an alpha anomeric nucleic acid).

[0053] The term “recombinant nucleic acid molecule” refers to a polynucleotide produced by human intervention. A recombinant nucleic acid molecule can contain two or more nucleotide sequences that are linked in a manner such that the product is not found in a cell in nature. In particular, the two or more nucleotide sequences can be operatively linked and, for example, can encode a fusion polypeptide, or can comprise a nucleotide sequence and a regulatory element. A recombinant nucleic acid molecule also can be based on, but different, from a naturally occurring polynucleotide, for example, a polynucleotide having one or more nucleotide changes such that a first codon, which normally is found in the polynucleotide, is replaced with a degenerate codon that encodes the same or a conservative amino acid, or such that a sequence of interest is introduced into the polynucleotide, for example, a restriction endonuclease recognition site or a splice site, a promoter, a DNA replication initiation site, or the like.

[0054] As used herein, the term “abiotic stress” or “abiotic stress condition” refers to the exposure of a plant, plant cell, or the like, to a non-living (“abiotic”) physical or chemical agent or condition that has an adverse effect on metabolism, growth, development, propagation and/or survival of the plant (collectively “growth”). An abiotic stress can be imposed on a plant due, for example, to an environmental factor such as water (e.g., flooding, drought, dehydration), anaerobic conditions (e.g., a low level of oxygen), abnormal osmotic conditions, salinity or temperature (e.g., hot/heat, cold, freezing, frost), a deficiency of nutrients or exposure to pollutants, or by a hormone, second messenger or other molecule. Anaerobic stress, for example, is due to a reduction in oxygen levels (hypoxia or anoxia) sufficient to produce a stress response. A flooding stress can be due to prolonged or transient immersion of a plant, plant part, tissue or isolated cell in a liquid medium such as occurs during monsoon, wet season, flash flooding or excessive irrigation of plants, or the like. A cold stress or heat stress can occur due to a decrease or increase, respectively, in the temperature from the optimum range of growth temperatures for a particular plant species. Such optimum growth temperature ranges are readily determined or known to those skilled in the art. Dehydration stress can be induced by the loss of water, reduced turgor, or reduced water content of a cell, tissue, organ or whole plant. Drought stress can be induced by or associated with the deprivation of water or reduced supply of water to a cell, tissue, organ or organism. Saline stress (salt stress) can be associated with or induced by a perturbation in the osmotic potential of the intracellular or extracellular environment of a cell. Osmotic stress also can be associated with or induced by a change, for example, in the concentration of molecules in the intracellular or extracellular environment of a plant cell, particularly where the molecules cannot be partitioned across the plant cell membrane.

[0055] As disclosed herein, clusters of plant stress-regulated genes (Example 1; see, also, Tables 1-31) and homologs and orthologs thereof (Table 32) have been identified. Remarkably, several of the stress-regulated genes previously were known to encode polypeptides having defined cellular functions, including roles as transcription factors, enzymes such as kinases, and structural proteins such as channel proteins (see Tables 29-31). The identification of Arabidopsis stress-regulated genes provides a means to identify homologous and orthologous genes and gene sequences in other plant species using well known procedures and algorithms based on identity (or homology) to the disclosed sequences. Thus, the invention provides polynucleotide sequences comprising plant stress-regulated genes that are homologs or orthologs, variants, or otherwise substantially similar to the polynucleotides disclosed herein, and having an E value≦1×10−8, which can be identified, for example, by a BLASTN search using the Arabidopsis polynucleotides of Tables 1 and 2 (SEQ ID NOS:1-5379) as query sequences (see Table 32, on CD).

[0056] A polynucleotide sequence of a stress-regulated gene as disclosed herein can be particularly useful for performing the methods of the invention on a variety of plants, including but not limited to, corn (Zea mays), Brassica sp. (e.g., B. napus, B. rapa, B. juncea), particularly those Brassica species useful as sources of seed oil, alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), millet (e.g., pearl millet (Pennisetum glaucum), proso millet (Panicum miliaceum), foxtail millet (Setaria italica), finger millet (Eleusine coracana)), sunflower (Helianthus annuus), safflower (Carthamus tinctorius), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium barbadense, Gossypium hirsutum), sweet potato (Ipomoea batatus), cassava (Manihot esculenta), coffee (Cofea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea ultilane), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), sugarcane (Saccharum spp.), oats, duckweed (Lemna), barley, tomatoes (Lycopersicon esculentum), lettuce (e.g., Lactuca sativa), green beans (Phaseolus vulgaris), lima beans (Phaseolus limensis), peas (Lathyrus spp.), and members of the genus Cucumis such as cucumber (C. sativus), cantaloupe (C. cantalupensis), and musk melon (C. melo).Ornamentals such as azalea (Rhododendron spp.), hydrangea (Macrophylla hydrangea), hibiscus (Hibiscus rosasanensis), roses (Rosa spp.), tulips (Tulipa spp.), daffodils (Narcissus spp.), petunias (Petunia hybrida), carnation (Dianthus caryophyllus), poinsettia (Euphorbia pulcherrima), and chrysanthemum are also included. Additional ornamentals within the scope of the invention include impatiens, Begonia, Pelargonium, Viola, Cyclamen, Verbena, Vinca, Tagetes, Primula, Saint Paulia, Agertum, Amaranthus, Antihirrhinum, Aquilegia, Cineraria, Clover, Cosmo, Cowpea, Dahlia, Datura, Delphinium, Gerbera, Gladiolus, Gloxinia, Hippeastrum, Mesembryanthemum, Salpiglossos, and Zinnia. Conifers that may be employed in practicing the present invention include, for example, pines such as loblolly pine (Pinus taeda), slash pine (Pinus elliotii), ponderosa pine (Pinus ponderosa), lodgepole pine (Pinus contorta), and Monterey pine (Pinus radiata), Douglas-fir (Pseudotsuga menziesii); Western hemlock (Tsuga ultilane); Sitka spruce (Picea glauca); redwood (Sequoia sempervirens); true firs such as silver fir (Abies amabilis) and balsam fir (Abies balsamea); and cedars such as Western red cedar (Thuja plicata) and Alaska yellow-cedar (Chamaecyparis nootkatensis).

[0057] Leguminous plants which may be used in the practice of the present invention include beans and peas. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mung bean, lima bean, fava bean, lentils, chickpea, etc. Legumes include, but are not limited to, Arachis, e.g., peanuts, Vicia, e.g., crown vetch, hairy vetch, adzuki bean, mung bean, and chickpea, Lupinus, e.g., lupine, trifolium, Phaseolus, e.g., common bean and lima bean, Pisum, e.g., field bean, Melilotus, e.g., clover, Medicago, e.g., alfalfa, Lotus, e.g., trefoil, lens, e.g., lentil, and false indigo. Preferred forage and turf grass for use in the methods of the invention include alfalfa, orchard grass, tall fescue, perennial ryegrass, creeping bent grass, and redtop.

[0058] Other plants within the scope of the invention include Acacia, aneth, artichoke, arugula, blackberry, canola, cilantro, clementines, escarole, eucalyptus, fennel, grapefruit, honey dew, jicama, kiwifruit, lemon, lime, mushroom, nut, okra, orange, parsley, persimmon, plantain, pomegranate, poplar, radiata pine, radicchio, Southern pine, sweetgum, tangerine, triticale, vine, yams, apple, pear, quince, cherry, apricot, melon, hemp, buckwheat, grape, raspberry, chenopodium, blueberry, nectarine, peach, plum, strawberry, watermelon, eggplant, pepper, cauliflower, Brassica, e.g., broccoli, cabbage, ultilan sprouts, onion, carrot, leek, beet, broad bean, celery, radish, pumpkin, endive, gourd, garlic, snapbean, spinach, squash, turnip, ultilane, chicory, groundnut and zucchini.

[0059] As used herein, the term “substantially similar”, when used herein with respect to a nucleotide sequence, means a nucleotide sequence corresponding to a reference nucleotide sequence, wherein the corresponding sequence encodes a polypeptide or comprises a regulatory element having substantially the same structure and function as the polypeptide encoded by the reference nucleotide sequence, for example, where only changes in amino acids not affecting the polypeptide function occur. For purposes of the present invention, a reference (or query) sequence is a polynucleotide sequence as set forth in any of SEQ ID NOS:1-2703 or a polypeptide encoded thereby. Desirably, a substantially similar nucleotide sequence encodes the polypeptide encoded by the reference nucleotide sequence. The percentage of identity between the substantially similar nucleotide sequence and the reference nucleotide sequence desirably is at least 60%, more desirably at least 75%, preferably at least 90%, more preferably at least 95%, still more preferably at least 99% and including 100%. A nucleotide sequence is “substantially similar” to reference nucleotide sequence hybridizes to the reference nucleotide sequence in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 2×SSC, 0.1% SDS at 50° C., more desirably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 1×SSC, 0.1% SDS at 50° C. (stringent conditions), more desirably still in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 0.5×SSC, 0.1% SDS at 50° C. (high stringency), preferably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 50° C. (very high stringency), more preferably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 65° C. (extremely high stringency).

[0060] In addition, the term “substantially similar,” when used in reference to a polypeptide sequence, means that an amino acid sequence relative to a reference (query) sequence shares at least about 65% amino acid sequence identity, particularly at least about 75% amino acid sequence identity, and preferably at least about 85%, more preferably at least about 90%, and most preferably at least about 95% or greater amino acid sequence identity. Generally, sequences having an E≦10−8 are considered to be substantially similar to a query sequence. Such sequence identity can take into account conservative amino acid changes that do not substantially affect the function of a polypeptide. As such, homologs or orthologs of the Arabidopsis stress-regulated nucleotide sequences disclosed herein, variants thereof, and polypeptides substantially similar to the polynucleotide sequence of Arabidopsis stress-regulated genes set forth in SEQ ID NOS:1-5379 are encompassed within the present invention and, therefore, useful for practicing the methods of the invention (see, for example, Table 32, which is on the CD-R filed herewith, and incorporated herein by reference).

[0061] Homology or identity is often measured using sequence analysis software such as the Sequence Analysis Software Package of the Genetics Computer Group (University of Wisconsin Biotechnology Center, 1710 University Avenue, Madison, Wis. 53705). Such software matches similar sequences by assigning degrees of homology to various deletions, substitutions and other modifications. The terms “homology” and “identity,” when used herein in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or of nucleotides that are the same when compared and aligned for maximum correspondence over a comparison window or designated region as measured using any number of sequence comparison algorithms or by manual alignment and visual inspection.

[0062] For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters.

[0063] The term “comparison window” is used broadly herein to include reference to a segment of any one of the number of contiguous positions, for example, about 20 to 600 positions, for example, amino acid or nucleotide position, usually about 50 to about 200 positions, more usually about 100 to about 150 positions, in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequence for comparison are well-known in the art. Optimal alignment of sequences for comparison can be conducted, for example, by the local homology algorithm of Smith and Waterman (Adv. Appl. Math. 2:482, 1981), by the homology alignment algorithm of Needleman and Wunsch (J. Mol. Biol. 48:443, 1970), by the search for similarity method of Person and Lipman (Proc. Natl. Acad. Sci., USA 85:2444, 1988), each of which is incorporated herein by reference; by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.); or by manual alignment and visual inspection. Other algorithms for determining homology or identity include, for example, in addition to a BLAST program (Basic Local Alignment Search Tool at the National Center for Biological Information), ALIGN, AMAS (Analysis of Multiply Aligned Sequences), AMPS (Protein Multiple Sequence Alignment), ASSET (Aligned Segment Statistical Evaluation Tool), BANDS, BESTSCOR, BIOSCAN (Biological Sequence Comparative Analysis Node), BLIMPS (BLocks IMProved Searcher), FASTA, Intervals & Points, BMB, CLUSTAL V, CLUSTAL W, CONSENSUS, LCONSENSUS, WCONSENSUS, Smith-Waterman algorithm, DARWIN, Las Vegas algorithm, FNAT (Forced Nucleotide Alignment Tool), Framealign, Framesearch, DYNAMIC, FILTER, FSAP (Fristensky Sequence Analysis Package), GAP (Global Alignment Program), GENAL, GIBBS, GenQuest, ISSC (Sensitive Sequence Comparison), LALIGN (Local Sequence Alignment), LCP (Local Content Program), MACAW (Multiple Alignment Construction & Analysis Workbench), MAP (Multiple Alignment Program), MBLKP, MBLKN, PIMA (Pattern-Induced Multi-sequence Alignment), SAGA (Sequence Alignment by Genetic Algorithm) and WHAT-IF. Such alignment programs can also be used to screen genome databases to identify polynucleotide sequences having substantially identical sequences.

[0064] A number of genome databases are available for comparison. Several databases containing genomic information annotated with some functional information are maintained by different organizations, and are accessible via the internet, for example, at world wide web addresses (url's) “wwwtigr.org/tdb”; “genetics.wisc.edu”; “genome-www.stanford.edu/˜ball”; “hiv-web.lanl.gov”; “ncbi.nlm.nih.gov”; “ebi.ac.uk”; “Pasteur.fr/other/biology”; and “genome.wi.mit.edu”.

[0065] In particular, the BLAST and BLAST 2.0 algorithms using default parameters are particularly useful for identifying polynucleotide and polypeptides encompassed within the present invention (Altschul et al. (Nucleic Acids Res. 25:3389-3402, 1977; J. Mol. Biol. 215:403-410, 1990, each of which is incorporated herein by reference). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra, 1977, 1990). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, M=5, N=4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength of 3, and expectations (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff and Henikoff, Proc. Natl. Acad. Sci., USA 89:10915, 1989) alignments (B) of 50, expectation (E) of 10, M=5, N=4, and a comparison of both strands.

[0066] The BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, for example, Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873, 1993, which is incorporated herein by reference). One measure of similarity provided by BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a references sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01, and most preferably less than about 0.001. Significantly, upon identifying polynucleotides that are substantially similar to those of SEQ ID NOS:1-5379, the identified polynucleotides can be used as query sequences in a BLAST search to identify polynucleotides and polypeptides substantially similar thereto.

[0067] It should be noted that the nucleotide sequences set forth as SEQ ID NOS:1-2703 comprise coding sequences, whereas the nucleotide sequences set forth as SEQ ID NOS:2704-5379 comprise regulatory sequences. In addition, the coding sequences and regulatory sequences are related in that, for example, SEQ ID NO:1 is the coding sequence of a plant cold regulated gene having a 5′ upstream (regulatory) sequence set forth as SEQ ID NO:2704 (see Table 2). Similarly, SEQ ID NO:2705 comprises a regulatory region of SEQ ID NO:2, SEQ ID NO:2706 comprises a regulatory region of SEQ ID NO:3, and so forth as shown in Table 2. As such, reference herein, for example, to a “polynucleotide comprising SEQ ID NO:1” can, unless indicated otherwise, include at least SEQ ID NO:2704. In some cases, the entire coding region of a plant stress regulated gene or the 5′ upstream sequence has not yet been determined (see, for example, SEQ ID NO:43 in Table 3, where “none” indicates that 5′ upstream regulatory sequences have not yet been determined). However, the determination of a complete coding sequence where only a portion is known or of regulatory sequences where a portion of the coding sequence is known can be made using methods as disclosed herein or otherwise known in the art.

[0068] In one embodiment, protein and nucleic acid sequence homologies are evaluated using the Basic Local Alignment Search Tool (“BLAST”). In particular, five specific BLAST programs are used to perform the following task:

[0069] (1) BLASTP and BLAST3 compare an amino acid query sequence against a protein sequence database;

[0070] (2) BLASTN compares a nucleotide query sequence against a nucleotide sequence database;

[0071] (3) BLASTX compares the six-frame conceptual translation products of a query nucleotide sequence (both strands) against a protein sequence database;

[0072] (4) TBLASTN compares a query protein sequence against a nucleotide sequence database translated in all six reading frames (both strands); and

[0073] (5) TBLASTX compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database.

[0074] The BLAST programs identify homologous sequences by identifying similar segments, which are referred to herein as “high-scoring segment pairs,” between a query amino or nucleic acid sequence and a test sequence which is preferably obtained from a protein or nucleic acid sequence database. High-scoring segment pairs are preferably identified (i.e., aligned) by means of a scoring matrix, many of which are known in the art. Preferably, the scoring matrix used is the BLOSUM62 matrix (Gonnet et al., Science 256:1443-1445, 1992; Henikoff and Henikoff, Proteins 17:49-61, 1993, each of which is incorporated herein by reference). Less preferably, the PAM or PAM250 matrices may also be used (Schwartz and Dayhoff, eds., “Matrices for Detecting Distance Relationships: Atlas of Protein Sequence and Structure” (Washington, National Biomedical Research Foundation 1978)). BLAST programs are accessible through the U.S. National Library of Medicine, for example, on the world wide web at address (url) “ncbi.nlm.nih.gov”.

[0075] The parameters used with the above algorithms may be adapted depending on the sequence length and degree of homology studied. In some embodiments, the parameters may be the default parameters used by the algorithms in the absence of instructions from the user.

[0076] The term “substantially similar” also is used in reference to a comparison of expression profiles of nucleotide sequences, wherein a determination that an expression profile characteristic of a stress response is substantially similar to the profile of nucleic acid molecules expressed in a plant cell being examined (“test plant”) is indicative of exposure of the test plant cell to one or a combination of abiotic stress conditions. When used in reference to such a comparison of expression profiles, the term “substantially similar” means that the individual nucleotide sequences in the test plant cell profile are altered in the same manner as the corresponding nucleotide sequences in the expression profile characteristic of the stress response.

[0077] By way of example, where exposure to saline results in an increased expression of nucleotide sequences A, B and C, and a decreased expression of nucleotide sequences D and E, as indicated by the expression profile characteristic of a saline stress response, a determination that corresponding nucleotide sequences A, B and C in the test plant cell are increased and that nucleotides sequences D and E are decreased is indicative of exposure of the test plant cell to a saline stress condition. It should be recognized that, where, for example, only nucleotide sequences A, B, D and E are examined in the test plant cell, an increase in A and B and a decrease in D and E expression of the test plant cells is considered to be substantially similar to the expression profile characteristic of a saline stress condition and, therefore, is indicative of exposure of the plant cell to a saline stress condition. Similarly, where the levels of expression of the nucleotide sequences examined in a test plant are altered in the same manner, i.e., are increased or are decreased, as that observed in an expression profile characteristic of a particular stress response, the absolute levels of expression may vary, for example, two-fold, five-fold, ten-fold, or the like. Nevertheless, the expression profile of the test plant cell is considered to be substantially similar to the expression profile characteristic of the particular stress response and, therefore, indicative of exposure of the plant cell to the stress condition.

[0078] As disclosed herein, clusters of stress-regulated genes (and their products), some of which also have been described as having cellular functions such as enzymatic activity or roles as transcription factors, are involved in the response of plant cells to various abiotic stresses (see Tables 29-31; see, also, Tables 1 and 32). As such, the polynucleotide sequences comprising the genes in a cluster likely share common stress-regulated regulatory elements, including, for example, cold-regulated regulatory elements (SEQ ID NOS:2704-3955), salinity-regulated regulatory elements (SEQ ID NOS:4910-5107, and osmotic pressure-regulated regulatory elements (SEQ ID NO:5108-5263), as well as regulatory elements that are responsive to a combination of stress conditions, but not to any of the individual stress conditions, alone (SEQ ID NOS:3956-4909 and 5263-5379). The identification of such clusters of genes thus provides a means to identify the stress-regulated regulatory elements that control the level of expression of these genes.

[0079] As used herein, the term “plant stress-regulated gene” means a polynucleotide sequence of a plant, the transcription of which is altered in response to exposure to a stress condition, and the regulatory elements linked to such a polynucleotide sequence and involved in the stress response, which can be induction or repression. In general, plant stress gene regulatory elements are contained within a sequence including approximately two kilobases upstream (5′) of the transcription or translation start site and two kilobases downstream (3′) of the transcription or translation termination site. In the absence of an abiotic stress condition, the stress-regulated gene can normally be unexpressed in the cells, can be expressed at a basal level, which is induced to a higher level in response to the stress condition, or can be expressed at a level that is reduced (decreased) in response to the stress condition. The coding region of a plant stress-regulated gene encodes a stress-regulated polypeptide, and also can be the basis for expression of a functional RNA molecule such as an antisense molecule or ribozyme. A stress-regulated polypeptide can have an adaptive effect on a plant, thereby allowing the plant to better tolerate stress conditions; or can have a maladaptive effect, thereby decreasing the ability of the plant to tolerate the stress conditions.

[0080] The present invention provides an isolated plant stress-regulated regulatory element, which regulates expression of an operatively linked nucleotide sequence in a plant in response a stress condition. As disclosed herein, a plant stress-regulated regulatory element can be isolated from a polynucleotide sequence of a plant stress-regulated gene comprising a nucleotide sequence as set forth in SEQ ID NOS:1-2703, for example any of SEQ ID NOS:2704-5379 (see Table 2). It is recognized that certain of the polynucleotides set forth as SEQ ID NOS:1-5379 previously have been described as being involved in a stress-regulated response in plants, including SEQ ID NOS:156, 229, 233, 558, 573, 606, 625, 635, 787, 813, 1263, 1386, 1391, 1405, 1445, 1484, 1589, 1609, 1634, 1726, 1866, 1918, and 1928 and, therefore, are not encompassed, in whole or in part, within the compositions of the invention, and are encompassed within only certain particular methods of the invention, for example, methods of making a transgenic plant that is resistant to two or more stress conditions, since, even where such a gene was known to be expressed in response to a single stress condition such as cold or saline (e.g., SEQ ID NO:1263), it was not known prior to the present disclosure that any of these genes was responsive to a combination of stress conditions (for example, a combination of cold and osmotic stress for SEQ ID NOS:1726, 1866, 1918, and 1928; or a combination of cold, osmotic and saline stress for SEQ ID NOS:1263, 1386, 1391, 1405, 1445, 1484, 1589, 1609, and 1634).

[0081] Methods for identifying and isolating the stress-regulated regulatory element from the disclosed polynucleotides, or genomic DNA clones corresponding thereto, are well known in the art. For example, methods of making deletion constructs or linker-scanner constructs can be used to identify nucleotide sequences that are responsive to a stress condition. Generally, such constructs include a reporter gene operatively linked to the sequence to be examined for regulatory activity. By performing such assays, a plant stress-regulated regulatory element can be defined within a sequence of about 500 nucleotides or fewer, generally at least about 200 nucleotides or fewer, particularly about 50 to 100 nucleotides, and more particularly at least about 20 nucleotides or fewer. Preferably the minimal (core) sequence required for regulating a stress response of a plant is identified.

[0082] The nucleotide sequences of the genes of a cluster also can be examined using a homology search engine such as described herein to identify sequences of conserved identity, particularly in the nucleotide sequence upstream of the transcription start site. Since all of the genes in a cluster as disclosed are induced in response to a particular stress condition or a particular combination of stress conditions, some or all of the nucleotide sequences can share conserved stress-regulated regulatory elements. By performing such a homology search, putative stress-regulated regulatory elements can be identified. The ability of such identified sequences to function as a plant stress-regulated regulatory element can be confirmed, for example, by operatively linking the sequence to a reporter gene and assaying the construct for responsiveness to a stress condition.

[0083] As used herein, the term “regulatory element” means a nucleotide sequence that, when operatively linked to a coding region of a gene, effects transcription of the coding region such that a ribonucleic acid (RNA) molecule is transcribed from the coding region. A regulatory element generally can increase or decrease the amount of transcription of a nucleotide sequence, for example, a coding sequence, operatively linked to the element with respect to the level at which the nucleotide sequence would be transcribed absent the regulatory element. Regulatory elements are well known in the art and include promoters, enhancers, silencers, inactivated silencer intron sequences, 3′-untranslated or 5′-untranslated sequences of transcribed sequence, for example, a poly-A signal sequence, or other protein or RNA stabilizing elements, or other gene expression control elements known to regulate gene expression or the amount of expression of a gene product. A regulatory element can be isolated from a naturally occurring genomic DNA sequence or can be synthetic, for example, a synthetic promoter.

[0084] Regulatory elements can be constitutively expressed regulatory element, which maintain gene expression at a relative level of activity (basal level), or can be regulated regulatory elements. Constitutively expressed regulatory elements can be expressed in any cell type, or can be tissue specific, which are expressed only in particular cell types, phase specific, which are expressed only during particular developmental or growth stages of a plant cell, or the like. A regulatory element such as a tissue specific or phase specific regulatory element or an inducible regulatory element useful in constructing a recombinant polynucleotide or in a practicing a method of the invention can be a regulatory element that generally, in nature, is found in a plant genome. However, the regulatory element also can be from an organism other than a plant, including, for example, from a plant virus, an animal virus, or a cell from an animal or other multicellular organism.

[0085] A regulatory element useful for practicing method of the present is a promoter element. Useful promoters include, but are not limited to, constitutive, inducible, temporally regulated, developmentally regulated, spatially-regulated, chemically regulated, stress-responsive, tissue-specific, viral and synthetic promoters. Promoter sequences are known to be strong or weak. A strong promoter provides for a high level of gene expression, whereas a weak promoter provides for a very low level of gene expression. An inducible promoter is a promoter that provides for the turning on and off of gene expression in response to an exogenously added agent, or to an environmental or developmental stimulus. A bacterial promoter such as the Ptac promoter can be induced to varying levels of gene expression depending on the level of isothiopropylgalactoside added to the transformed bacterial cells. An isolated promoter sequence that is a strong promoter for heterologous nucleic acid is advantageous because it provides for a sufficient level of gene expression to allow for easy detection and selection of transformed cells and provides for a high level of gene expression when desired.

[0086] Within a plant promoter region there are several domains that are necessary for full function of the promoter. The first of these domains lies immediately upstream of the structural gene and forms the “core promoter region” containing consensus sequences, normally 70 base pairs immediately upstream of the gene. The core promoter region contains the characteristic CAAT and TATA boxes plus surrounding sequences, and represents a transcription initiation sequence that defines the transcription start point for the structural gene.

[0087] The presence of the core promoter region defines a sequence as being a promoter: if the region is absent, the promoter is non-functional. The core promoter region, however, is insufficient to provide full promoter activity. A series of regulatory sequences upstream of the core constitute the remainder of the promoter. These regulatory sequences determine expression level, the spatial and temporal pattern of expression and, for an important subset of promoters, expression under inductive conditions (regulation by external factors such as light, temperature, chemicals, hormones).

[0088] To define a minimal promoter region, a DNA segment representing the promoter region is removed from the 5′ region of the gene of interest and operably linked to the coding sequence of a marker (reporter) gene by recombinant DNA techniques well known to the art. The reporter gene is operably linked downstream of the promoter, so that transcripts initiating at the promoter proceed through the reporter gene. Reporter genes generally encode proteins which are easily measured, including, but not limited to, chloramphenicol acetyl transferase (CAT), beta-glucuronidase (GUS), green fluorescent protein (GFP), β-galactosidase (β-GAL), and luciferase.

[0089] The construct containing the reporter gene under the control of the promoter is then introduced into an appropriate cell type by transfection techniques well known to the art. To assay for the reporter protein, cell lysates are prepared and appropriate assays, which are well known in the art, for the reporter protein are performed. For example, if CAT were the reporter gene of choice, the lysates from cells transfected with constructs containing CAT under the control of a promoter under study are mixed with isotopically labeled chloramphenicol and acetyl-coenzyme A (acetyl-CoA). The CAT enzyme transfers the acetyl group from acetyl-CoA to the 2-position or 3-position of chloramphenicol. The reaction is monitored by thin layer chromatography, which separates acetylated chloramphenicol from unreacted material. The reaction products are then visualized by autoradiography.

[0090] The level of enzyme activity corresponds to the amount of enzyme that was made, which in turn reveals the level of expression from the promoter of interest. This level of expression can be compared to other promoters to determine the relative strength of the promoter under study. In order to be sure that the level of expression is determined by the promoter, rather than by the stability of the mRNA, the level of the reporter mRNA can be measured directly, for example, by northern blot analysis.

[0091] Once activity is detected, mutational and/or deletional analyses may be employed to determine the minimal region and/or sequences required to initiate transcription. Thus, sequences can be deleted at the 5′ end of the promoter region and/or at the 3′ end of the promoter region, and nucleotide substitutions introduced. These constructs are then introduced to cells and their activity determined.

[0092] The choice of promoter will vary depending on the temporal and spatial requirements for expression, and also depending on the target species. In some cases, expression in multiple tissues is desirable. While in others, tissue-specific, e.g., leaf-specific, seed-specific, petal-specific, anther-specific, or pith-specific, expression is desirable. Although many promoters from dicotyledons have been shown to be operational in monocotyledons and vice versa, ideally dicotyledonous promoters are selected for expression in dicotyledons, and monocotyledonous promoters for expression in monocotyledons. There is, however, no restriction to the origin or source of a selected promoter. It is sufficient that the promoters are operational in driving the expression of a desired nucleotide sequence in the particular cell.

[0093] A range of naturally-occurring promoters are known to be operative in plants and have been used to drive the expression of heterologous (both foreign and endogenous) genes and nucleotide sequences in plants: for example, the constitutive 35S cauliflower mosaic virus (CaMV) promoter, the ripening-enhanced tomato polygalacturonase promoter (Bird et al., 1988), the E8 promoter (Diekman and Fischer, 1988) and the fruit specific 2A1 promoter (Pear et al., 1989). Many other promoters, e.g., U2 and U5 snRNA promoters from maize, the promoter from alcohol dehydrogenase, the Z4 promoter from a gene encoding the Z4 22 kD zein protein, the Z10 promoter from a gene encoding a 10 kD zein protein, a Z27 promoter from a gene encoding a 27 kD zein protein, the A20 promoter from the gene encoding a 19 kD zein protein, inducible promoters, such as the light inducible promoter derived from the pea rbcS gene and the actin promoter from rice, e.g., the actin 2 promoter (WO 00/70067); seed specific promoters, such as the phaseolin promoter from beans, may also be used. The nucleotide sequences of the stress-regulated genes of this invention can also be expressed under the regulation of promoters that are chemically regulated. This enables the nucleic acid sequence or encoded polypeptide to be synthesized only when the crop plants are treated with the inducing chemicals. Chemical induction of gene expression is detailed in EP 0 332 104 and U.S. Pat. No. 5,614,395.

[0094] In some instances it may be desirable to link a constitutive promoter to a polynucleotide comprising a stress regulated gene of the invention. Examples of some constitutive promoters include the rice actin 1 (Wang et al., 1992; U.S. Pat. No. 5,641,876), CaMV 35S (Odell et al., 1985), CaMV 19S (Lawton et al., 1987), nos, Adh, sucrose synthase; and the ubiquitin promoters.

[0095] In other situations it may be desirable to limit expression of stress-related sequences to specific tissues or stages of development. As used herein, the term “tissue specific or phase specific regulatory element” means a nucleotide sequence that effects transcription in only one or a few cell types, or only during one or a few stages of the life cycle of a plant, for example, only for a period of time during a particular stage of growth, development or differentiation. The terms “tissue specific” and “phase specific” are used together herein in referring to a regulatory element because a single regulatory element can have characteristics of both types of regulatory elements. For example, a regulatory element active only during a particular stage of plant development also can be expressed only in one or a few types of cells in the plant during the particular stage of development. As such, any attempt to classify such regulatory elements as tissue specific or as phase specific can be difficult. Accordingly, unless indicated otherwise, all regulatory elements having the characteristic of a tissue specific regulatory element, or a phase specific regulatory element, or both are considered together for purposes of the present invention.

[0096] Examples of tissue specific promoters which have been described include the lectin (Vodkin, 1983; Lindstrom et al., 1990) corn alcohol dehydrogenase 1 (Vogel et al., 1989; Dennis et al., 1984), corn light harvesting complex (Simpson, 1986; Bansal et al., 1992), corn heat shock protein (Odell et al., 1985), pea small subunit RuBP carboxylase (Poulsen et al., 1986), Ti plasmid mannopine synthase and Ti plasmid nopaline synthase (Langridge et al., 1989), petunia chalcone isomerase (vanTunen et al., 1988), bean glycine rich protein 1 (Keller et al., 1989), truncated CaMV 35s (Odell et al., 1985), potato patatin (Wenzler et al., 1989), root cell (Yamamoto et al., 1990), maize zein (Reina et al., 1990; Kriz et al., 1987; Wandelt et al., 1989; Langridge et al., 1983; Reina et al., 1990), globulin-1 (Belanger et al., 1991), α-tubulin, cab (Sullivan et al., 1989), PEPCase (Hudspeth & Grula, 1989), R gene complex-associated promoters (Chandler et al., 1989), histone, and chalcone synthase promoters (Franken et al., 1991). Tissue specific enhancers are described by Fromm et al. (1989).

[0097] Several other tissue-specific regulated genes and/or promoters have been reported in plants, including genes encoding seed storage proteins such as napin, cruciferin, beta-conglycinin, and phaseolin, zein or oil body proteins such as oleosin, genes involved in fatty acid biosynthesis, including acyl carrier protein, stearoyl-ACP desaturase, fatty acid desaturases (fad 2-1), and other genes expressed during embryonic development such as Bce4 (see, for example, EP 255378 and Kridl et al., 1991). Particularly useful for seed-specific expression is the pea vicilin promoter (Czako et al., 1992). (See also U.S. Pat. No. 5,625,136, which is incorporated herein by reference.) Other useful promoters for expression in mature leaves are those that are switched on at the onset of senescence, such as the SAG promoter from Arabidopsis (Gan et al., 1995).

[0098] A class of fruit-specific promoters expressed at or during antithesis through fruit development, at least until the beginning of ripening, is discussed in U.S. Pat. No. 4,943,674. cDNA clones that are preferentially expressed in cotton fiber have been isolated (John et al., 1992). cDNA clones from tomato displaying differential expression during fruit development have been isolated and characterized (Mansson et al., 1985, Slater et al., 1985). The promoter for polygalacturonase gene is active in fruit ripening. The polygalacturonase gene is described in U.S. Pat. Nos. 4,535,060, 4,769,061, 4,801,590, and 5,107,065, each of which is incorporated herein by reference.

[0099] Other examples of tissue-specific promoters include those that direct expression in leaf cells following damage to the leaf (for example, from chewing insects), in tubers (for example, patatin gene promoter), and in fiber cells (an example of a developmentally-regulated fiber cell protein is E6 (John et al., 1992). The E6 gene is most active in fiber, although low levels of transcripts are found in leaf, ovule and flower.

[0100] Additional tissue specific or phase specific regulatory elements include, for example, the AGL8/FRUITFULL regulatory element, which is activated upon floral induction (Hempel et al., Development 124:3845-3853, 1997, which is incorporated herein by reference); root specific regulatory elements such as the regulatory elements from the RCP1 gene and the LRP1 gene (Tsugeki and Fedoroff, Proc. Natl. Acad., USA 96:12941-12946, 1999; Smith and Fedoroff, Plant Cell 7:735-745, 1995, each of which is incorporated herein by reference); flower specific regulatory elements such as the regulatory elements from the LEAFY gene and the APETELA1 gene (Blazquez et al., Development 124:3835-3844, 1997, which is incorporated herein by reference; Hempel et al., supra, 1997); seed specific regulatory elements such as the regulatory element from the oleosin gene (Plant et al., Plant Mol. Biol. 25:193-205, 1994, which is incorporated herein by reference), and dehiscence zone specific regulatory element. Additional tissue specific or phase specific regulatory elements include the Zn13 promoter, which is a pollen specific promoter (Hamilton et al., Plant Mol. Biol. 18:211-218, 1992, which is incorporated herein by reference); the UNUSUAL FLORAL ORGANS (UFO) promoter, which is active in apical shoot meristem; the promoter active in shoot meristems (Atanassova et al., Plant J. 2:291, 1992, which is incorporated herein by reference), the cdc2a promoter and cyc07 promoter (see, for example, Ito et al., Plant Mol. Biol. 24:863, 1994; Martinez et al., Proc. Natl. Acad. Sci., USA 89:7360, 1992; Medford et al., Plant Cell 3:359, 1991; Terada et al., Plant J. 3:241, 1993; Wissenbach et al., Plant J. 4:411, 1993, each of which is incorporated herein by reference); the promoter of the APETELA3 gene, which is active in floral meristems (Jack et al., Cell 76:703, 1994, which is incorporated herein by reference; Hempel et al., supra, 1997); a promoter of an agamous-like (AGL) family member, for example, AGL8, which is active in shoot meristem upon the transition to flowering (Hempel et al., supra, 1997); floral abscission zone promoters; L1-specific promoters; and the like.

[0101] The tissue-specificity of some “tissue-specific” promoters may not be absolute and may be tested by one skilled in the art using the diphtheria toxin sequence. One can also achieve tissue-specific expression with “leaky” expression by a combination of different tissue-specific promoters (Beals et al., 1997). Other tissue-specific promoters can be isolated by one skilled in the art (see U.S. Pat. No. 5,589,379). Several inducible promoters (“gene switches”) have been reported, many of which are described in the review by Gatz (1996) and Gatz (1997). These include tetracycline repressor system, Lac repressor system, copper inducible systems, salicylate inducible systems (such as the PR1a system), glucocorticoid (Aoyama et al., 1997) and ecdysone inducible systems. Also included are the benzene sulphonamide (U.S. Pat. No. 5,364,780) and alcohol (WO 97/06269 and WO 97/06268) inducible systems and glutathione S-transferase promoters.

[0102] In some instances it might be desirable to inhibit expression of a native DNA sequence within a plant's tissues to achieve a desired phenotype. In this case, such inhibition might be accomplished with transformation of the plant to comprise a constitutive, tissue-independent promoter operably linked to an antisense nucleotide sequence, such that constitutive expression of the antisense sequence produces an RNA transcript that interferes with translation of the mRNA of the native DNA sequence.

[0103] Inducible regulatory elements also are useful for purposes of the present invention. As used herein, the term “inducible regulatory element” means a regulatory element that, when exposed to an inducing agent, effects an increased level of transcription of a nucleotide sequence to which it is operatively linked as compared to the level of transcription, if any, in the absence of an inducing agent. Inducible regulatory elements can be those that have no basal or constitutive activity and only effect transcription upon exposure to an inducing agent, or those that effect a basal or constitutive level of transcription, which is increased upon exposure to an inducing agent. Inducible regulatory elements that effect a basal or constitutive level of expression generally are useful in a method or composition of the invention where the induced level of transcription is substantially greater than the basal or constitutive level of expression, for example, at least about two-fold greater, or at least about five-fold greater. Particularly useful inducible regulatory elements do not have a basal or constitutive activity, or increase the level of transcription at least about ten-fold greater than a basal or constitutive level of transcription associated with the regulatory element.

[0104] Inducible promoters that have been described include the ABA- and turgor-inducible promoters, the promoter of the auxin-binding protein gene (Schwob et al., 1993), the UDP glucose flavonoid glycosyl-transferase gene promoter (Ralston et al., 1988), the MPI proteinase inhibitor promoter (Cordero et al., 1994), and the glyceraldehyde-3-phosphate dehydrogenase gene promoter (Kohler et al., 1995; Quigley et al., 1989; Martinez et al., 1989).

[0105] The term “inducing agent” is used to refer to a chemical, biological or physical agent or environmental condition that effects transcription from an inducible regulatory element. In response to exposure to an inducing agent, transcription from the inducible regulatory element generally is initiated de novo or is increased above a basal or constitutive level of expression. Such induction can be identified using the methods disclosed herein, including detecting an increased level of RNA transcribed from a nucleotide sequence operatively linked to the regulatory element, increased expression of a polypeptide encoded by the nucleotide sequence, or a phenotype conferred by expression of the encoded polypeptide.

[0106] An inducing agent useful in a method of the invention is selected based on the particular inducible regulatory element. For example, the inducible regulatory element can be a metallothionein regulatory element, a copper inducible regulatory element or a tetracycline inducible regulatory element, the transcription from which can be effected in response to metal ions, copper or tetracycline, respectively (Furst et al., Cell 55:705-717, 1988; Mett et al., Proc. Natl. Acad. Sci. USA 90:4567-4571, 1993; Gatz et al., Plant J. 2:397-404, 1992; Roder et al., Mol. Gen. Genet. 243:32-38, 1994, each of which is incorporated herein by reference). The inducible regulatory element also can be an ecdysone regulatory element or a glucocorticoid regulatory element, the transcription from which can be effected in response to ecdysone or other steroid (Christopherson et al., Proc. Natl. Acad. Sci., USA 89:6314-6318, 1992; Schena et al., Proc. Natl. Acad. Sci., USA 88:10421-10425, 1991, each of which is incorporated herein by reference). In addition, the regulatory element can be a cold responsive regulatory element or a heat shock regulatory element, the transcription of which can be effected in response to exposure to cold or heat, respectively (Takahashi et al., Plant Physiol. 99:383-390, 1992, which is incorporated herein by reference). Additional regulatory elements useful in the methods or compositions of the invention include, for example, the spinach nitrite reductase gene regulatory element (Back et al., Plant Mol. Biol. 17:9, 1991, which is incorporated herein by reference); a light inducible regulatory element (Feinbaum et al., Mol. Gen. Genet. 226:449, 1991; Lam and Chua, Science 248:471, 1990, each of which is incorporated herein by reference), a plant hormone inducible regulatory element (Yamaguchi-Shinozaki et al., Plant Mol. Biol. 15:905, 1990; Kares et al., Plant Mol. Biol. 15:225, 1990, each of which is incorporated herein by reference), and the like.

[0107] An inducible regulatory element also can be a plant stress-regulated regulatory element of the invention. In addition to the known stress conditions that specifically induce or repress expression from such elements, the present invention provides methods of identifying agents that mimic a stress condition. Accordingly, such stress mimics are considered inducing or repressing agents with respect to a plant stress-regulated regulatory element. In addition, a recombinant polypeptide comprising a zinc finger domain, which is specific for the regulatory element, and an effector domain, particularly an activator, can be useful as an inducing agent for a plant stress-regulated regulatory element. Furthermore, such a recombinant polypeptide provides the advantage that the effector domain can be a repressor domain, thereby providing a repressing agent, which decreases expression from the regulatory element. In addition, use of such a method of modulating expression of an endogenous plant stress-regulated gene provides the advantage that the polynucleotide encoding the recombinant polypeptide can be introduced into cells of the plant, thus providing a transgenic plant that can be regulated coordinately with the endogenous plant stress-regulated gene upon exposure to a stress condition. A polynucleotide encoding such a recombinant polypeptide can be operatively linked to and expressed from a constitutively active, inducible or tissue specific or phase specific regulatory element.

[0108] In one embodiment, the promoter may be a gamma zein promoter, an oleosin ole16 promoter, a globulin I promoter, an actin I promoter, an actin c1 promoter, a sucrose synthetase promoter, an INOPS promoter, an EXM5 promoter, a globulin2 promoter, a b-32, ADPG-pyrophosphorylase promoter, an LtpI promoter, an Ltp2 promoter, an oleosin ole17 promoter, an oleosin ole18 promoter, an actin 2 promoter, a pollen-specific protein promoter, a pollen-specific pectate lyase promoter, an anther-specific protein promoter (Huffman), an anther-specific gene RTS2 promoter, a pollen-specific gene promoter, a tapeturn-specific gene promoter, tapeturn-specific gene RAB24 promoter, a anthranilate synthase alpha subunit promoter, an alpha zein promoter, an anthranilate synthase beta subunit promoter, a dihydrodipicolinate synthase promoter, a Thi 1 promoter, an alcohol dehydrogenase promoter, a cab binding protein promoter, an H3C4 promoter, a RUBISCO SS starch branching enzyme promoter, an ACCase promoter, an actin3 promoter, an actin7 promoter, a regulatory protein GF14-12 promoter, a ribosomal protein L9 promoter, a cellulose biosynthetic enzyme promoter, an S-adenosyl-L-homocysteine hydrolase promoter, a superoxide dismutase promoter, a C-kinase receptor promoter, a phosphoglycerate mutase promoter, a root-specific RCc3 mRNA promoter, a glucose-6 phosphate isomerase promoter, a pyrophosphate-fructose 6-phosphate-1-phosphotransferase promoter, an ubiquitin promoter, a beta-ketoacyl-ACP synthase promoter, a 33 kDa photosystem 11 promoter, an oxygen evolving protein promoter, a 69 kDa vacuolar ATPase subunit promoter, a metallothionein-like protein promoter, a glyceraldehyde-3-phosphate dehydrogenase promoter, an ABA- and ripening-inducible-like protein promoter, a phenylalanine ammonia lyase promoter, an adenosine triphosphatase S-adenosyl-L-homocysteine hydrolase promoter, an a-tubulin promoter, a cab promoter, a PEPCase promoter, an R gene promoter, a lectin promoter, a light harvesting complex promoter, a heat shock protein promoter, a chalcone synthase promoter, a zein promoter, a globulin-1 promoter, an ABA promoter, an auxin-binding protein promoter, a UDP glucose flavonoid glycosyl-transferase gene promoter, an NTI promoter, an actin promoter, an opaque 2 promoter, a b70 promoter, an oleosin promoter, a CaMV 35S promoter, a CaMV 19S promoter, a histone promoter, a turgor-inducible promoter, a pea small subunit RuBP carboxylase promoter, a Ti plasmid mannopine synthase promoter, Ti plasmid nopaline synthase promoter, a petunia chalcone isomerase promoter, a bean glycine rich protein I promoter, a CaMV 35S transcript promoter, a potato patatin promoter, or a S-E9 small subunit RuBP carboxylase promoter.

[0109] In addition to promoters, a variety of 5′ and 3′ transcriptional regulatory sequences are also available for use in the present invention. Transcriptional terminators are responsible for the termination of transcription and correct mRNA polyadenylation. The 3′-untranslated regulatory DNA sequence preferably includes from about 50 to about 1,000, more preferably about 100 to about 1,000, nucleotide base pairs and contains plant transcriptional and translational termination sequences. Appropriate transcriptional terminators and those which are known to function in plants include the CaMV 35S terminator, the tml terminator, the nopaline synthase terminator, the pea rbcS E9 terminator, the terminator for the T7 transcript from the octopine synthase gene of Agrobacterium tumefaciens, and the 3′ end of the protease inhibitor I or II genes from potato or tomato, although other 340 elements known to those of skill in the art can also be employed. Alternatively, one also could use a gamma coixin, oleosin 3 or other terminator from the genus Coix. Preferred 3′ elements include those from the nopaline synthase gene of Agrobacterium tumefaciens (Bevan et al., 1983), the terminator for the T7 transcript from the octopine synthase gene of Agrobacterium tumefaciens, and the 3′ end of the protease inhibitor I or II genes from potato or tomato.

[0110] As the DNA sequence between the transcription initiation site and the start of the coding sequence, i.e., the untranslated leader sequence, can influence gene expression, one may also wish to employ a particular leader sequence. Preferred leader sequences are contemplated to include those that include sequences predicted to direct optimum expression of the attached sequence, i.e., to include a preferred consensus leader sequence that may increase or maintain mRNA stability and prevent inappropriate initiation of translation. The choice of such sequences will be known to those of skill in the art in light of the present disclosure. Sequences that are derived from genes that are highly expressed in plants will be most preferred.

[0111] Other sequences that have been found to enhance gene expression in transgenic plants include intron sequences (e.g., from Adh1, bronze1, actin1, actin 2 (WO 00/760067), or the sucrose synthase intron) and viral leader sequences (e.g., from TMV, MCMV and AMV). For example, a number of non-translated leader sequences derived from viruses are known to enhance expression. Specifically, leader sequences from tobacco mosaic virus (TMV), maize chlorotic mottle virus (MCMV), and alfalfa mosaic virus (AMV) have been shown to be effective in enhancing expression (e.g., Gallie et al., 1987; Skuzeski et al., 1990). Other leaders known in the art include but are not limited to picornavirus leaders, for example, EMCV leader (encephalomyocarditis virus 5′ non-coding region; Elroy-Stein et al., 1989); potyvirus leaders, for example, TEV leader (tobacco etch virus); MDMV leader (maize dwarf mosaic virus); human immunoglobulin heavy chain binding protein (BiP) leader, (Macejak et al., 1991); untranslated leader from the coat protein mRNA of AMV (AMV RNA 4; Jobling et al., 1987), TMV (Gallie et al., 1989), and MCMV (Lommel et al., 1991; see also, della Cioppa et al., 1987).

[0112] Regulatory elements such as Adh intron 1 (Callis et al., 1987), sucrose synthase intron (Vasil et al., 1989) or TMV omega element (Gallie, et al., 1989), may further be included where desired. Examples of enhancers include elements from the CaMV 35S promoter, octopine synthase genes (Ellis et al., 1987), the rice actin 1 gene, the maize alcohol dehydrogenase gene (Callis et al., 1987), the maize shrunken I gene (Vasil et al., 1989), TMV Omega element (Gallie et al., 1989) and promoters from non-plant eukaryotes (e.g. yeast; Ma et al., 1988).

[0113] Vectors for use in accordance with the present invention may be constructed to include the ocs enhancer element, which was first identified as a 16 bp palindromic enhancer from the octopine synthase (ocs) gene of ultilane (Ellis et al., 1987), and is present in at least 10 other promoters (Bouchez et al., 1989). The use of an enhancer element, such as the ocs element and particularly multiple copies of the element, will act to increase the level of transcription from adjacent promoters when applied in the context of monocot transformation.

[0114] The methods of the invention provide genetically modified plant cells, which can contain, for example, a coding region, or peptide portion thereof, of a plant stress-regulated gene operatively linked to a heterologous inducible regulatory element; or a plant stress-regulated regulatory element operatively linked to a heterologous nucleotide sequence encoding a polypeptide of interest. In such a plant, the expression from the inducible regulatory element can be effected by exposing the plant cells to an inducing agent in any of numerous ways depending, for example, on the inducible regulatory element and the inducing agent. For example, where the inducible regulatory element is a cold responsive regulatory element present in the cells of a transgenic plant, the plant can be exposed to cold conditions, which can be produced artificially, for example, by placing the plant in a thermostatically controlled room, or naturally, for example, by planting the plant in an environment characterized, at least in part, by attaining temperatures sufficient to induce transcription from the promoter but not so cold as to kill the plants. By examining the phenotype of such transgenic plants, those plants that ectopically express a gene product that confers increased resistance of the plant to cold can be identified. Similarly, a transgenic plant containing a metallothionein promoter can be exposed to metal ions such as cadmium or copper by watering the plants with a solution containing the inducing metal ions, or can be planted in soil that is contaminated with a level of such metal ions that is toxic to most plants. The phenotype of surviving plants can be observed, those expressing desirable traits can be selected.

[0115] As used herein, the term “phenotype” refers to a physically detectable characteristic. A phenotype can be identified visually by inspecting the physical appearance of a plant following exposure, for example, to increased osmotic conditions; can be identified using an assay to detecting a product produced due to expression of reporter gene, for example, an RNA molecule, a polypeptide such as an enzyme, or other detectable signal such as disclosed herein; or by using any appropriate tool useful for identifying a phenotype of a plant, for example, a microscope, a fluorescence activated cell sorter, or the like.

[0116] A transgenic plant containing an inducible regulatory element such as a steroid inducible regulatory element can be exposed to a steroid by watering the plants with a solution containing the steroid. The use of an inducible regulatory element that is induced upon exposure to a chemical or biological inducing agent that can be placed in solution or suspension in an aqueous medium can be particularly useful because the inducing agent can be applied conveniently to a relatively large crop of transgenic plants containing the inducible regulatory element, for example, through a watering system or by spraying the inducing agent over the field. As such, inducible regulatory elements that are responsive to an environmental inducing agent, for example, cold; heat; metal ions or other potentially toxic agents such as a pesticides, which can contaminate a soil; or the like; or inducible regulatory elements that are regulated by inducing agents that conveniently can be applied to plants, can be particularly useful in a method or composition of the invention, and allow the identification and selection of plants that express desirable traits and survive and grow in environments that otherwise would not support growth of the plants.

[0117] As disclosed herein, the present invention provides plant stress-regulated regulatory elements, which are identified based on the expression of clusters of plant genes in response to stress. As used herein, the term “stress-regulated regulatory element of a plant” or “plant stress-regulated regulatory element” means a nucleotide sequence of a plant genome that can respond to a stress such that expression of a gene product encoded by a gene comprising the regulatory element (a stress-inducible gene) is increased above or decreased below the level of expression of the gene product in the absence of the stress condition. The regulatory element can be any gene regulatory element, including, for example, a promoter, an enhancer, a silencer, or the like. In one embodiment, the plant stress-regulated regulatory element is a plant stress-regulated promoter.

[0118] For purposes of modulating the responsiveness of a plant to a stress condition, it can be useful to introduce a modified plant stress-regulated regulatory element into a plant. Such a modified regulatory element can have any desirable characteristic, for example, it can be inducible to a greater level than the corresponding wild-type promoter, or it can be inactivated such that, upon exposure to a stress, there is little or no induction of expression of a nucleotide sequence operatively linked to the mutant element. A plant stress-regulated regulatory element can be modified by incorporating random mutations using, for example, in vitro recombination or DNA shuffling (Stemmer et al., Nature 370: 389-391, 1994; U.S. Pat. No. 5,605,793, each of which is incorporated herein by reference). Using such a method, millions of mutant copies of the polynucleotide, for example, stress-regulated regulatory element, can be produced based on the original nucleotide sequence, and variants with improved properties, such as increased inducibility can be recovered.

[0119] A mutation method such as DNA shuffling encompasses forming a mutagenized double-stranded polynucleotide from a template double-stranded polynucleotide, wherein the template double-stranded polynucleotide has been cleaved into double stranded random fragments of a desired size, and comprises the steps of adding to the resultant population of double-stranded random fragments one or more single or double stranded oligonucleotides, wherein the oligonucleotides comprise an area of identity and an area of heterology to the double stranded template polynucleotide; denaturing the resultant mixture of double stranded random fragments and oligonucleotides into single stranded fragments; incubating the resultant population of single stranded fragments with a polymerase under conditions that result in the annealing of the single stranded fragments at the areas of identity to form pairs of annealed fragments, the areas of identity being sufficient for one member of a pair to prime replication of the other, thereby forming a mutagenized double-stranded polynucleotide; and repeating the second and third steps for at least two further cycles, wherein the resultant mixture in the second step of a further cycle includes the mutagenized double-stranded polynucleotide from the third step of the previous cycle, and the further cycle forms a further mutagenized double-stranded polynucleotide. Preferably, the concentration of a single species of double stranded random fragment in the population of double stranded random fragments is less than 1% by weight of the total DNA. In addition, the template double stranded polynucleotide can comprise at least about 100 species of polynucleotides. The size of the double stranded random fragments can be from about 5 base pairs to 5 kilobase pairs. In a further embodiment, the fourth step of the method comprises repeating the second and the third steps for at least 10 cycles.

[0120] A plant stress-regulated regulatory element of the invention is useful for expressing a nucleotide sequence operatively linked to the element in a cell, particularly a plant cell. As used herein, the term “expression” refers to the transcription and/or translation of an endogenous gene or a transgene in plants. In the case of an antisense molecule, for example, the term “expression” refers to the transcription of the polynucleotide encoding the antisense molecule.

[0121] As used herein, the term “operatively linked,” when used in reference to a plant stress-regulated regulatory element, means that the regulatory element is positioned with respect to a second nucleotide sequence such that the regulatory element effects transcription or transcription and translation of the nucleotide sequence in substantially the same manner, but not necessarily to the same extent, as it does when the regulatory element is present in its natural position in a genome. Transcriptional promoters, for example, generally act in a position and orientation dependent manner and usually are positioned at or within about five nucleotides to about fifty nucleotides 5′ (upstream) of the start site of transcription of a gene in nature. In comparison, enhancers and silencers can act in a relatively position or orientation independent manner and, therefore, can be positioned several hundred or thousand nucleotides upstream or downstream from a transcription start site, or in an intron within the coding region of a gene, yet still be operatively linked to a coding region so as to effect transcription.

[0122] The second nucleotide sequence, i.e., the sequence operatively linked to the plant stress-regulated regulatory element, can be any nucleotide sequence, including, for example, a coding region of a gene or cDNA; a sequence encoding an antisense molecule, an RNAi molecule, ribozyme, triplexing agent (see, for example, Frank-Kamenetskii and Mirkin, Ann. Rev. Biochem. 64:65-95, 1995), or the like; or a sequence that, when transcribed, can be detected in the cell using, for example, by hybridization or amplification, or when translated produces a detectable signal. The term “coding region” is used broadly herein to include a nucleotide sequence of a genomic DNA or a cDNA molecule comprising all or part of a coding region of the coding strand. A coding region can be transcribed from an operatively linked regulatory element, and can be translated into a full length polypeptide or a peptide portion of a polypeptide. It should be recognized that, in a nucleotide sequence comprising a coding region, not all of the nucleotides in the sequence need necessarily encode the polypeptide and, particularly, that a gene transcript can contain one or more introns, which do not encode an amino acid sequence of a polypeptide but, nevertheless, are part of the coding region, particularly the coding strand, of the gene.

[0123] The present invention also relates to a recombinant polynucleotide, which contains a polynucleotide portion of a plant stress-regulated gene operatively linked to a heterologous nucleotide sequence. As used herein, the term “polynucleotide portion of plant stress-regulated sequence” means a contiguous nucleotide sequence of the plant stress-regulated gene that provides a function. The portion can be any portion of the sequence, particularly a coding sequence, or a sequence encoding a peptide portion of the stress-regulated polypeptide; the stress-regulated regulatory element; a sequence useful as an antisense molecule or triplexing agent; or a sequence useful for disrupting (knocking-out) an endogenous plant stress-regulated gene.

[0124] A heterologous nucleotide sequence is a nucleotide sequence that is not normally part of the plant stress-regulated gene from which the polynucleotide portion of the plant stress-regulated gene-component of the recombinant polynucleotide is obtained; or, if it is a part of the plant stress-regulated gene from which the polynucleotide portion is obtained, it is an orientation other than it would normally be in, for example, is an antisense sequence, or comprises at least partially discontinuous as compared to the genomic structure, for example, a single exon operatively linked to the regulatory element. In general, where the polynucleotide portion of the plant stress-regulated gene comprises the coding sequence in a recombinant polynucleotide of the invention, the heterologous nucleotide sequence will function as a regulatory element. The regulatory element can be any heterologous regulatory element, including, for example, a constitutively active regulatory element, an inducible regulatory element, or a tissue specific or phase specific regulatory element, as disclosed above. Conversely, where the polynucleotide portion of the plant stress-regulated polynucleotide comprises the stress-regulated regulatory element of a recombinant polynucleotide of the invention, the heterologous nucleotide sequence generally will be a nucleotide sequence that can be transcribed and, if desired, translated. Where the heterologous nucleotide sequence is expressed from a plant stress-regulated regulatory element, it generally confers a desirable phenotype to a plant cell containing the recombinant polynucleotide, or provides a means to identify a plant cell containing the recombinant polynucleotide. It should be recognized that a “desirable” phenotype can be one that decreases the ability of a plant cell to compete where the plant cell, or a plant containing the cell, is an undesired plant cell. Thus, a heterologous nucleotide sequence can allow a plant to grow, for example, under conditions in which it would not normally be able to grow.

[0125] A heterologous nucleotide sequence can be, or encode, a selectable marker. As used herein, the term “selectable marker” is used herein to refer to a molecule that, when present or expressed in a plant cell, provides a means to identify a plant cell containing the marker. As such, a selectable marker can provide a means for screening a population of plants, or plant cells, to identify those having the marker. A selectable marker also can confer a selective advantage to the plant cell, or a plant containing the cell. The selective advantage can be, for example, the ability to grow in the presence of a negative selective agent such as an antibiotic or herbicide, compared to the growth of plant cells that do not contain the selectable marker. The selective advantage also can be due, for example, to an enhanced or novel capacity to utilize an added compound as a nutrient, growth factor or energy source. A selectable advantage can be conferred, for example, by a single polynucleotide, or its expression product, or to a combination of polynucleotides whose expression in a plant cell gives the cell with a positive selective advantage, a negative selective advantage, or both.

[0126] Examples of selectable markers include those that confer antimetabolite resistance, for example, dihydrofolate reductase, which confers resistance to methotrexate (Reiss, Plant Physiol. (Life Sci. Adv.) 13:143-149, 1994); neomycin phosphotransferase, which confers resistance to the aminoglycosides neomycin, kanamycin and paromycin (Herrera-Estrella, EMBO J. 2:987-995, 1983) and hygro, which confers resistance to hygromycin (Marsh, Gene 32:481-485, 1984), trpB, which allows cells to utilize indole in place of tryptophan; hisD, which allows cells to utilize histinol in place of histidine (Hartman, Proc. Natl. Acad. Sci., USA 85:8047, 1988); mannose-6-phosphate isomerase which allows cells to utilize mannose (WO 94/20627); omithine decarboxylase, which confers resistance to the ornithine decarboxylase inhibitor, 2-(difluoromethyl)-DL-omithine (DFMO; McConlogue, 1987, In: Current Communications in Molecular Biology, Cold Spring Harbor Laboratory ed.); and deaminase from Aspergillus terreus, which confers resistance to Blasticidin S (Tamura, Biosci. Biotechnol. Biochem. 59:2336-2338, 1995). Additional selectable markers include those that confer herbicide resistance, for example, phosphinothricin acetyltransferase gene, which confers resistance to phosphinothricin (White et al., Nucl. Acids Res. 18:1062, 1990; Spencer et al., Theor. Appl. Genet. 79:625-631, 1990), a mutant EPSPV-synthase, which confers glyphosate resistance (Hinchee et al., Bio/Technology 91:915-922, 1998), a mutant acetolactate synthase, which confers imidazolione or sulfonylurea resistance (Lee et al., EMBO J. 7:1241-1248, 1988), a mutant psbA, which confers resistance to atrazine (Smeda et al., Plant Physiol. 103:911-917, 1993), or a mutant protoporphyrinogen oxidase (see U.S. Pat. No. 5,767,373), or other markers conferring resistance to an herbicide such as glufosinate. In addition, markers that facilitate identification of a plant cell containing the polynucleotide encoding the marker include, for example, luciferase (Giacomin, Plant Sci. 116:59-72, 1996; Scikantha, J. Bacteriol. 178:121, 1996), green fluorescent protein (Gerdes, FEBS Lett. 389:44-47, 1996) or fl-glucuronidase (Jefferson, EMBO J. 6:3901-3907, 1997), and numerous others as disclosed herein or otherwise known in the art. Such markers also can be used as reporter molecules.

[0127] A heterologous nucleotide sequence can encode an antisense molecule, particularly an antisense molecule specific for a nucleotide sequence of a plant stress-regulated gene, for example, the gene from which the regulatory component of the recombinant polynucleotide is derived. Such a recombinant polynucleotide can be useful for reducing the expression of a plant stress-regulated polypeptide in response to a stress condition because the antisense molecule, like the polypeptide, only will be induced upon exposure to the stress. A heterologous nucleotide sequence also can be, or can encode, a ribozyme or a triplexing agent. In addition to being useful as heterologous nucleotide sequences, such molecules also can be used directly in a method of the invention, for example, to modulate the responsiveness of a plant cell to a stress condition. Thus, an antisense molecule, ribozyme, or triplexing agent can be contacted directly with a target cell and, upon uptake by the cell, can effect their antisense, ribozyme or triplexing activity; or can be encoded by a heterologous nucleotide sequence that is expressed in a plant cell from a plant stress-regulated regulatory element, whereupon it can effect its activity.

[0128] An antisense polynucleotide, ribozyme or triplexing agent is complementary to a target sequence, which can be a DNA or RNA sequence, for example, messenger RNA, and can be a coding sequence, a nucleotide sequence comprising an intron-exon junction, a regulatory sequence such as a Shine-Delgamo-like sequence, or the like. The degree of complementarity is such that the polynucleotide, for example, an antisense polynucleotide, can interact specifically with the target sequence in a cell. Depending on the total length of the antisense or other polynucleotide, one or a few mismatches with respect to the target sequence can be tolerated without losing the specificity of the polynucleotide for its target sequence. Thus, few if any mismatches would be tolerated in an antisense molecule consisting, for example, of twenty nucleotides, whereas several mismatches will not affect the hybridization efficiency of an antisense molecule that is complementary, for example, to the full length of a target mRNA encoding a cellular polypeptide. The number of mismatches that can be tolerated can be estimated, for example, using well known formulas for determining hybridization kinetics (see Sambrook et al., “Molecular Cloning; A Laboratory Manual” 2nd Edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; 1989)) or can be determined empirically using methods as disclosed herein or otherwise known in the art, particularly by determining that the presence of the antisense polynucleotide, ribozyme, or triplexing agent in a cell decreases the level of the target sequence or the expression of a polypeptide encoded by the target sequence in the cell.

[0129] A nucleotide sequence useful as an antisense molecule, a ribozyme or a triplexing agent can inhibit translation or cleave a polynucleotide encoded by plant stress-regulated gene, thereby modulating the responsiveness of a plant cell to a stress condition. An antisense molecule, for example, can bind to an mRNA to form a double stranded molecule that cannot be translated in a cell. Antisense oligonucleotides of at least about 15 to 25 nucleotides are preferred since they are easily synthesized and can hybridize specifically with a target sequence, although longer antisense molecules can be expressed from a recombinant polynucleotide introduced into the target cell. Specific nucleotide sequences useful as antisense molecules can be identified using well known methods, for example, gene walking methods (see, for example, Seimiya et al., J. Biol. Chem. 272:4631-4636 (1997), which is incorporated herein by reference). Where the antisense molecule is contacted directly with a target cell, it can be operatively associated with a chemically reactive group such as iron-linked EDTA, which cleaves a target RNA at the site of hybridization. A triplexing agent, in comparison, can stall transcription (Maher et al., Antisense Res. Devel. 1:227 (1991); Helene, Anticancer Drug Design 6:569 (1991)).

[0130] A plant stress-regulated regulatory element can be included in an expression cassette. As used herein, the term “expression cassette” refers to a nucleotide sequence that can direct expression of an operatively linked polynucleotide. Thus, a plant stress-regulated regulatory element can constitute an expression cassette, or component thereof. An expression cassette is particularly useful for directing expression of a nucleotide sequence, which can be an endogenous nucleotide sequence or a heterologous nucleotide sequence, in a cell, particularly a plant cell. If desired, an expression cassette also can contain additional regulatory elements, for example, nucleotide sequences required for proper translation of a polynucleotide sequence into a polypeptide. In general, an expression cassette can be introduced into a plant cell such that the plant cell, a plant resulting from the plant cell, seeds obtained from such a plant, or plants produced from such seeds are resistant to a stress condition.

[0131] Additional regulatory sequences as disclosed above or other desirable sequences such as selectable markers or the like can be incorporated into an expression cassette containing a plant stress-regulated regulatory element (see, for example, WO 99/47552). Examples of suitable markers include dihydrofolate reductase (DHFR) or neomycin resistance for eukaryotic cells and tetracycline or ampicillin resistance for E. coli. Selection markers in plants include bleomycin, gentamycin, glyphosate, hygromycin, kanamycin, methotrexate, phleomycin, phosphinotricin, spectinomycin, streptomycin, sulfonamide and sulfonylureas resistance (see, for example, Maliga et al., Methods in Plant Molecular Biology, Cold Spring Harbor Laboratory Press, 1995, page 39). The selection marker can have its own promoter or its expression can be driven by the promoter operably linked to the sequence of interest. Additional sequences such as intron sequences (e.g. from Adh1 or bronze1) or viral leader sequences (e.g. from TMV, MCMV and AIVIV), all of which can enhance expression, can be included in the cassette. In addition, where it is desirable to target expression of a nucleotide sequence operatively linked to the stress-regulated regulatory element, a sequence encoding a cellular localization motif can be included in the cassette, for example, such that an encoded transcript or translation product is translocated to and localizes in the cytosol, nucleus, a chloroplast, or another subcellular organelle. Examples of useful transit peptides and transit peptide sequences can be found in Von Heijne et al., Plant Mol. Biol. Rep. 9: 104, 1991; Clark et al., J. Biol. Chem. 264:17544, 1989; della Cioppa et al., Plant Physiol. 84:965, 1987; Romer et al., Biochem. Biophys. Res. Comm. 196:1414, 1993; Shah et al., Science 233:478, 1986; Archer et al., J. Bioenerg Biomemb. 22:789, 1990; Scandalios, Prog. Clin. Biol. Res. 344:515, 1990; Weisbeek et al., J. Cell Sci. Suppl. 11: 199, 1989; Bruce, Trends Cell Biol. 10:440, 2000. The present invention can utilize native or heterologous transit peptides. The encoding sequence for a transit peptide can include all or a portion of the encoding sequence for a particular transit peptide, and may also contain portions of the mature protein encoding sequence associated with a particular transit peptide.

[0132] A polynucleotide portion of a plant stress-regulated plant gene, or an expression cassette, can be introduced into a cell as a naked DNA molecule, can be incorporated in a matrix such as a liposome or a particle such as a viral particle, or can be incorporated into a vector. Such vectors can be cloning or expression vectors, but other uses are within the scope of the present invention. A cloning vector is a self-replicating DNA molecule that serves to transfer a DNA segment into a host cell. The three most common types of cloning vectors are bacterial plasmids, phages, and other viruses. An expression vector is a cloning vector designed so that a coding sequence inserted at a particular site will be transcribed and translated into a protein. Incorporation of the polynucleotide into a vector can facilitate manipulation of the polynucleotide, or introduction of the polynucleotide into a plant cell. A vector can be derived from a plasmid or a viral vector such as a T-DNA vector (Horsch et al., Science 227:1229-1231, 1985, which is incorporated herein by reference). If desired, the vector can comprise components of a plant transposable element, for example, a Ds transposon (Bancroft and Dean, Genetics 134:1221-1229, 1993, which is incorporated herein by reference) or an Spm transposon (Aarts et al., Mol. Gen. Genet. 247:555-564, 1995, which is incorporated herein by reference).

[0133] In addition to containing the polynucleotide portion of a plant stress-regulated gene, a vector can contain various nucleotide sequences that facilitate, for example, rescue of the vector from a transformed plant cell; passage of the vector in a host cell, which can be a plant, animal, bacterial, or insect host cell; or expression of an encoding nucleotide sequence in the vector, including all or a portion of a rescued coding region. As such, the vector can contain any of a number of additional transcription and translation elements, including constitutive and inducible promoters, enhancers, and the like (see, for example, Bitter et al., Meth. Enzymol. 153:516-544, 1987). For example, a vector can contain elements useful for passage, growth or expression in a bacterial system, including a bacterial origin of replication; a promoter, which can be an inducible promoter; and the like. In comparison, a vector that can be passaged in a mammalian host cell system can have a promoter such as a metallothionein promoter, which has characteristics of both a constitutive promoter and an inducible promoter, or a viral promoter such as a retrovirus long terminal repeat, an adenovirus late promoter, or the like. A vector also can contain one or more restriction endonuclease recognition and cleavage sites, including, for example, a polylinker sequence, to facilitate rescue of a nucleotide sequence operably linked to the polynucleotide portion.

[0134] The present invention also relates to a method of using a polynucleotide portion of a plant stress-regulated gene to confer a selective advantage on a plant cell. Such a method can be performed by introducing, for example, a plant stress-regulated regulatory element into a plant cell, wherein, upon exposure of the plant cell to a stress condition to which the regulatory element is responsive, a nucleotide sequence operatively linked to the regulatory element is expressed, thereby conferring a selective advantage to plant cell. The operatively linked nucleotide sequence can be a heterologous nucleotide sequence, which can be operatively linked to the regulatory element prior to introduction of the regulatory sequence into the plant cell; or can be an endogenous nucleotide sequence into which the regulatory element was targeted by a method such as homologous recombination. The selective advantage conferred by the operatively linked nucleotide sequence can be such that the plant is better able to tolerate the stress condition; or can be any other selective advantage.

[0135] As used herein, the term “selective advantage” refers to the ability of a particular organism to better propagate, develop, grow, survive, or otherwise tolerate a condition as compared to a corresponding reference organism that does not contain a plant-stress regulated polynucleotide portion of the present invention. In one embodiment, a selective advantage is exemplified by the ability of a desired plant, plant cell, or the like, that contains an introduced plant stress-regulated regulatory element, to grow better than an undesired plant, plant cell, or the like, that does not contain the introduced regulatory element. For example, a recombinant polynucleotide comprising a plant stress-regulated regulatory element operatively linked to a heterologous nucleotide sequence encoding an enzyme that inactivates an herbicide can be introduced in a desired plant. Upon exposure of a mixed population of plants comprising the desired plants, which contain the recombinant polynucleotide, and one or more other populations of undesired plants, which lack the recombinant polynucleotide, to a stress condition that induces expression of the regulatory element and to the herbicide, the desired plants will have a greater likelihood of surviving exposure to the toxin and, therefore, a selective advantage over the undesired plants.

[0136] In another embodiment, a selective advantage is exemplified by the ability of a desired plant, plant cell, or the like, to better propagate, develop, grow, survive, or otherwise tolerate a condition as compared to an undesired plant, plant cell, or the like, that contains an introduced plant stress-regulated regulatory element. For example, a recombinant polynucleotide comprising a plant stress-regulated regulatory element operatively linked to a plant cell toxin can be introduced into cells of an undesirable plant present in a mixed population of desired and undesired plants, for example, food crops and weeds, respectively, then the plants can be exposed to stress conditions that induce expression from the plant stress-regulated regulatory element, whereby expression of the plant cell toxin results in inhibition of growth or death of the undesired plants, thereby providing a selective advantage to the desired plants, which no longer have to compete with the undesired plants for nutrients, light, or the like. In another example, a plant stress-regulated regulatory element operatively linked to a plant cell toxin can be introduced into cells of plants used as a nurse crop. Nurse crops, also called cover or companion crops, are planted in combination with plants of interest to provide, among other things, shade and soil stability during establishment of the desired plants. Once the desired plants have become established, the presence of the nurse crop may no longer be desirable. Exposure to conditions inducing expression of the gene linked to the plant stress-regulated regulatory element allows elimination of the nurse crop. Alternatively nurse crops can be made less tolerate to abiotic stress by the inhibition of any of the stress-regulated sequences disclosed herein. Inhibition can be accomplished by any of the method described herein. Upon exposure of the nurse crop to the stress, the decreased ability of the nurse crop to respond to the stress will result in elimination of the nurse crop, leaving only the desired plants.

[0137] The invention also provides a means of producing a transgenic plant, which comprises plant cells that exhibit altered responsiveness to a stress condition. As such, the present invention further provides a transgenic plant, or plant cells or tissues derived therefrom, which are genetically modified to respond to stress differently than a corresponding wild-type plant or plant not containing constructs of the present invention would respond. As used herein, the term “responsiveness to a stress condition” refers to the ability of a plant to express a plant stress-regulated gene upon exposure to the stress condition. A transgenic plant cell contains a polypeptide portion of a plant stress-regulated gene, or a mutant form thereof, for example, a knock-out mutant. A knock-out mutant form of a plant stress-regulated gene can contain, for example, a mutation such that a STOP codon is introduced into the reading frame of the translated portion of the gene such that expression of a functional stress-regulated polypeptide is prevented; or a mutation in the stress-regulated regulatory element such that inducibility of the element in response to a stress condition is inhibited. Such transgenic plants of the invention can display any of various idiotypic modifications is response to an abiotic stress, including altered tolerance to the stress condition, as well as increased or decreased plant growth, root growth, yield, or the like, as compared to the corresponding wild-type plant.

[0138] The term “plant” is used broadly herein to include any plant at any stage of development, or to part of a plant, including a plant cutting, a plant cell, a plant cell culture, a plant organ, a plant seed, and a plantlet. A plant cell is the structural and physiological unit of the plant, comprising a protoplast and a cell wall. A plant cell can be in the form of an isolated single cell or a cultured cell, or can be part of higher organized unit, for example, a plant tissue, plant organ, or plant. Thus, a plant cell can be a protoplast, a gamete producing cell, or a cell or collection of cells that can regenerate into a whole plant. As such, a seed, which comprises multiple plant cells and is capable of regenerating into a whole plant, is considered plant cell for purposes of this disclosure. A plant tissue or plant organ can be a seed, protoplast, callus, or any other groups of plant cells that is organized into a structural or functional unit. Particularly useful parts of a plant include harvestable parts and parts useful for propagation of progeny plants. A harvestable part of a plant can be any useful part of a plant, for example, flowers, pollen, seedlings, tubers, leaves, stems, fruit, seeds, roots, and the like. A part of a plant useful for propagation includes, for example, seeds, fruits, cuttings, seedlings, tubers, rootstocks, and the like.

[0139] A transgenic plant can be regenerated from a transformed plant cell. As used herein, the term “regenerate” means growing a whole plant from a plant cell; a group of plant cells; a protoplast; a seed; or a piece of a plant such as a callus or tissue. Regeneration from protoplasts varies from species to species of plants. For example, a suspension of protoplasts can be made and, in certain species, embryo formation can be induced from the protoplast suspension, to the stage of ripening and germination. The culture media generally contains various components necessary for growth and regeneration, including, for example, hormones such as auxins and cytokinins; and amino acids such as glutamic acid and proline, depending on the particular plant species. Efficient regeneration will depend, in part, on the medium, the genotype, and the history of the culture. If these variables are controlled, however, regeneration is reproducible.

[0140] Regeneration can occur from plant callus, explants, organs or plant parts. Transformation can be performed in the context of organ or plant part regeneration. (see Meth. Enzymol. Vol. 118; Klee et al. Ann. Rev. Plant Physiol. 38:467, 1987, which is incorporated herein by reference). Utilizing the leaf disk-transformation-regeneration method, for example, disks are cultured on selective media, followed by shoot formation in about two to four weeks (see Horsch et al., supra, 1985). Shoots that develop are excised from calli and transplanted to appropriate root-inducing selective medium. Rooted plantlets are transplanted to soil as soon as possible after roots appear. The plantlets can be repotted as required, until reaching maturity.

[0141] In vegetatively propagated crops, the mature transgenic plants are propagated utilizing cuttings or tissue culture techniques to produce multiple identical plants. Selection of desirable transgenotes is made and new varieties are obtained and propagated vegetatively for commercial use. In seed propagated crops, the mature transgenic plants can be self crossed to produce a homozygous inbred plant. The resulting inbred plant produces seeds that contain the introduced plant stress-induced regulatory element, and can be grown to produce plants that express a polynucleotide or polypeptide in response to a stress condition that induces expression from the regulatory element. As such, the invention further provides seeds produced by a transgenic plant obtained by a method of the invention.

[0142] In addition, transgenic plants comprising different recombinant sequences can be crossbred, thereby providing a means to obtain transgenic plants containing two or more different transgenes, each of which contributes a desirable characteristic to the plant. Methods for breeding plants and selecting for crossbred plants having desirable characteristics or other characteristics of interest are well known in the art.

[0143] A method of the invention can be performed by introducing a polynucleotide portion of a plant stress-regulated gene into the plant. As used herein, the term “introducing” means transferring a polynucleotide into a plant cell. A polynucleotide can be introduced into a cell by a variety of methods well known to those of ordinary skill in the art. For example, the polynucleotide can be introduced into a plant cell using a direct gene transfer method such as electroporation or microprojectile mediated transformation, or using Agrobacterium mediated transformation. Non-limiting examples of methods for the introduction of polynucleotides into plants are provided in greater detail herein. As used herein, the term “transformed” refers to a plant cell containing an exogenously introduced polynucleotide portion of a plant stress-regulated gene that is or can be rendered active in a plant cell, or to a plant comprising a plant cell containing such a polynucleotide.

[0144] It should be recognized that one or more polynucleotides, which are the same or different can be introduced into a plant, thereby providing a means to obtain a genetically modified plant containing multiple copies of a single transgenic sequence, or containing two or more different transgenic sequences, either or both of which can be present in multiple copies. Such transgenic plants can be produced, for example, by simply selecting plants having multiple copies of a single type of transgenic sequence; by cotransfecting plant cells with two or more populations of different transgenic sequences and identifying those containing the two or more different transgenic sequences; or by crossbreeding transgenic plants, each of which contains one or more desired transgenic sequences, and identifying those progeny having the desired sequences.

[0145] Methods for introducing a polynucleotide into a plant cell to obtain a transformed plant also include direct gene transfer (see European Patent A 164 575), injection, electroporation, biolistic methods such as particle bombardment, pollen-mediated transformation, plant RNA virus-mediated transformation, liposome-mediated transformation, transformation using wounded or enzyme-degraded immature embryos, or wounded or enzyme-degraded embryogenic callus, and the like. Transformation methods using Agrobacterium tumefaciens tumor inducing (Ti) plasmids or root-inducing (Ri) plasmids, or plant virus vectors are well known in the art (see, for example, WO 99/47552; Weissbach & Weissbach, “Methods for Plant Molecular Biology” (Academic Press, NY 1988), section VIII, pages 421-463; Grierson and Corey, “Plant Molecular Bioloy” 2d Ed. (Blackie, London 1988), Chapters 7-9, each of which is incorporated herein by reference; Horsch et al., supra, 1985). The wild-type form of Agrobacterium, for example, contains a Ti plasmid, which directs production of tumorigenic crown gall growth on host plants. Transfer of the tumor inducing T-DNA region of the Ti plasmid to a plant genome requires the Ti plasmid-encoded virulence genes as well as T-DNA borders, which are a set of direct DNA repeats that delineate the region to be transferred. An Agrobacterium based vector is a modified form of a Ti plasmid, in which the tumor inducing functions are replaced by a nucleotide sequence of interest that is to be introduced into the plant host.

[0146] Methods of using Agrobacterium mediated transformation include cocultivation of Agrobacterium with cultured isolated protoplasts; transformation of plant cells or tissues with Agrobacterium; and transformation of seeds, apices or meristems with Agrobacterium. In addition, in planta transformation by Agrobacterium can be performed using vacuum infiltration of a suspension of Agrobacterium cells (Bechtold et al., C.R. Acad. Sci. Paris 316:1194, 1993, which is incorporated herein by reference).

[0147] Agrobacterium mediated transformation can employ cointegrate vectors or binary vector systems, in which the components of the Ti plasmid are divided between a helper vector, which resides permanently in the Agrobacterium host and carries the virulence genes, and a shuttle vector, which contains the gene of interest bounded by T-DNA sequences. Binary vectors are well known in the art (see, for example, De Framond, BioTechnology 1:262, 1983; Hoekema et al., Nature 303:179, 1983, each of which is incorporated herein by reference) and are commercially available (Clontech; Palo Alto Calif.). For transformation, Agrobacterium can be cocultured, for example, with plant cells or wounded tissue such as leaf tissue, root explants, hypocotyledons, stem pieces or tubers (see, for example, Glick and Thompson, “Methods in Plant Molecular Biology and Biotechnology” (Boca Raton Fla., CRC Press 1993), which is incorporated herein by reference). Wounded cells within the plant tissue that have been infected by Agrobacterium can develop organs de novo when cultured under the appropriate conditions; the resulting transgenic shoots eventually give rise to transgenic plants, which contain an exogenous polynucleotide portion of a plant stress-regulated gene.

[0148] Agrobacterium mediated transformation has been used to produce a variety of transgenic plants, including, for example, transgenic cruciferous plants such as Arabidopsis, mustard, rapeseed and flax; transgenic leguminous plants such as alfalfa, pea, soybean, trefoil and white clover; and transgenic solanaceous plants such as eggplant, petunia, potato, tobacco and tomato (see, for example, Wang et al., “Transformation of Plants and Soil Microorganisms” (Cambridge, University Press 1995), which is incorporated herein by reference). In addition, Agrobacterium mediated transformation can be used to introduce an exogenous polynucleotide sequence, for example, a plant stress-regulated regulatory element into apple, aspen, belladonna, black currant, carrot, celery, cotton, cucumber, grape, horseradish, lettuce, morning glory, muskmelon, neem, poplar, strawberry, sugar beet, sunflower, walnut, asparagus, rice and other plants (see, for example, Glick and Thompson, supra, 1993; Hiei et al., Plant J. 6:271-282, 1994; Shimamoto, Science 270:1772-1773, 1995).

[0149] Suitable strains of Agrobacterium tumefaciens and vectors as well as transformation of Agrobacteria and appropriate growth and selection media are well known in the art (GV3101, pMK90RK), Koncz, Mol. Gen. Genet. 204:383-396, 1986; (C58C1, pGV3850kan), Deblaere, Nucl. Acid Res. 13:4777, 1985; Bevan, Nucl. Acid Res. 12:8711, 1984; Koncz, Proc. Natl. Acad. Sci. USA 86:8467-8471, 1986; Koncz, Plant Mol. Biol. 20:963-976, 1992; Koncz, Specialized vectors for gene tagging and expression studies. In: Plant Molecular Biology Manual Vol. 2, Gelvin and Schilperoort (Eds.), Dordrecht, The Netherlands: Kluwer Academic Publ. (1994), 1-22; European Patent A-1 20 516; Hoekema: The Binary Plant Vector System, Offsetdrukkerij Kanters B. V., Alblasserdam (1985), Chapter V; Fraley, Crit. Rev. Plant. Sci., 4:1-46; An, EMBO J. 4:277-287, 1985).

[0150] Where a polynucleotide portion of a plant stress-regulated gene is contained in vector, the vector can contain functional elements, for example “left border” and “right border” sequences of the T-DNA of Agrobacterium, which allow for stable integration into a plant genome. Furthermore, methods and vectors that permit the generation of marker-free transgenic plants, for example, where a selectable marker gene is lost at a certain stage of plant development or plant breeding, are known, and include, for example, methods of co-transformation (Lyznik, Plant Mol. Biol. 13:151-161, 1989; Peng, Plant Mol. Biol. 27:91-104, 1995), or methods that utilize enzymes capable of promoting homologous recombination in plants (see, e.g., W097/08331; Bayley, Plant Mol. Biol. 18:353-361, 1992; Lloyd, Mol. Gen. Genet. 242:653-657, 1994; Maeser, Mol. Gen. Genet. 230:170-176, 1991; Onouchi, Nucl. Acids Res. 19:6373-6378, 1991; see, also, Sambrook et al., supra, 1989).

[0151] A direct gene transfer method such as electroporation also can be used to introduce a polynucleotide portion of a plant stress-regulated gene into a cell such as a plant cell. For example, plant protoplasts can be electroporated in the presence of the regulatory element, which can be in a vector (Fromm et al., Proc. Natl. Acad. Sci., USA 82:5824, 1985, which is incorporated herein by reference). Electrical impulses of high field strength reversibly permeabilize membranes allowing the introduction of the nucleic acid. Electroporated plant protoplasts reform the cell wall, divide and form a plant callus. Microinjection can be performed as described in Potrykus and Spangenberg (eds.), Gene Transfer To Plants (Springer Verlag, Berlin, N.Y. 1995). A transformed plant cell containing the introduced polynucleotide can be identified by detecting a phenotype due to the introduced polynucleotide, for example, increased or decreased tolerance to a stress condition.

[0152] Microprojectile mediated transformation also can be used to introduce a polynucleotide into a plant cell (Klein et al., Nature 327:70-73, 1987, which is incorporated herein by reference). This method utilizes microprojectiles such as gold or tungsten, which are coated with the desired nucleic acid molecule by precipitation with calcium chloride, spermidine or polyethylene glycol. The microprojectile particles are accelerated at high speed into a plant tissue using a device such as the BIOLISTIC PD-1000 (BioRad; Hercules Calif.).

[0153] Microprojectile mediated delivery (“particle bombardment”) is especially useful to transform plant cells that are difficult to transform or regenerate using other methods. Methods for the transformation using biolistic methods are well known (Wan, Plant Physiol. 104:37-48, 1984; Vasil, Bio/Technology 11:1553-1558, 1993; Christou, Trends in Plant Science 1:423-431, 1996). Microprojectile mediated transformation has been used, for example, to generate a variety of transgenic plant species, including cotton, tobacco, corn, hybrid poplar and papaya (see Glick and Thompson, supra, 1993). Important cereal crops such as wheat, oat, barley, sorghum and rice also have been transformed using microprojectile mediated delivery (Duan et al., Nature Biotech. 14:494-498, 1996; Shimamoto, Curr. Opin. Biotech. 5:158-162, 1994). A rapid transformation regeneration system for the production of transgenic plants such as a system that produces transgenic wheat in two to three months (see European Patent No. EP 0709462A2, which is incorporated herein by reference) also can be useful for producing a transgenic plant using a method of the invention, thus allowing more rapid identification of gene functions. The transformation of most dicotyledonous plants is possible with the methods described above. Transformation of monocotyledonous plants also can be transformed using, for example, biolistic methods as described above, protoplast transformation, electroporation of partially permeabilized cells, introduction of DNA using glass fibers, Agrobacterium mediated transformation, and the like.

[0154] Plastid transformation also can be used to introduce a polynucleotide portion of a plant stress-regulated gene into a plant cell (U.S. Pat. Nos. 5,451,513, 5,545,817, and 5,545,818; WO 95/16783; McBride et al., Proc. Natl. Acad. Sci., USA 91:7301-7305, 1994). Chloroplast transformation involves introducing regions of cloned plastid DNA flanking a desired nucleotide sequence, for example, a selectable marker together with polynucleotide of interest into a suitable target tissue, using, for example, a biolistic or protoplast transformation method (e.g., calcium chloride or PEG mediated transformation). One to 1.5 kb flanking regions (“targeting sequences”) facilitate homologous recombination with the plastid genome, and allow the replacement or modification of specific regions of the plastome. Using this method, point mutations in the chloroplast 16S rRNA and rps12 genes, which confer resistance to spectinomycin and streptomycin, can be utilized as selectable markers for transformation (Svab et al., Proc. Natl. Acad. Sci., USA 87:8526-8530, 1990; Staub and Maliga, Plant Cell 4:39-45, 1992), resulted in stable homopiasmic transformants; at a frequency of approximately one per 100 bombardments of target leaves. The presence of cloning sites between these markers allowed creation of a plastid targeting vector for introduction of foreign genes (Staub and Maliga, EMBO J. 12:601-606, 1993). Substantial increases in transformation frequency are obtained by replacement of the recessive rRNA or r-protein antibiotic resistance genes with a dominant selectable marker, the bacterial aadA gene encoding the spectinomycin-detoxifying enzyme aminoglycoside-3′-adenyltransferase (Svab and Maliga, Proc. Natl. Acad. Sci., USA 90:913-917, 1993). Approximately 15 to 20 cell division cycles following transformation are generally required to reach a homoplastidic state. Plastid expression, in which genes are inserted by homologous recombination into all of the several thousand copies of the circular plastid genome present in each plant cell, takes advantage of the enormous copy number advantage over nuclear-expressed genes to permit expression levels that can readily exceed 10% of the total soluble plant protein.

[0155] Plants suitable to treatment according to a method of the invention can be monocots or dicots and include, but are not limited to, corn (Zea mays), Brassica sp. (e.g., B. napus, B. rapa, B. juncea), particularly those Brassica species useful as sources of seed oil, alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), millet (e.g., pearl millet (Pennisetum glaucum), proso millet (Panicum miliaceum), foxtail millet (Setaria italica), finger millet (Eleusine coracana)), sunflower (Helianthus annuus), safflower (Carthamus tinctorius), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium barbadense, Gossypium hirsutum), sweet potato (Ipomoea batatus), cassava (Manihot esculenta), coffee (Cofea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea ultilane), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), sugarcane (Saccharum spp.), oats, duckweed (Lemna), barley, tomatoes (Lycopersicon esculentum), lettuce (e.g., Lactuca sativa), green beans (Phaseolus vulgaris), lima beans (Phaseolus limensis), peas (Lathyrus spp.), and members of the genus Cucumis such as cucumber (C. sativus), cantaloupe (C. cantalupensis), and musk melon (C. melo).

[0156] Ornamentals such as azalea (Rhododendron spp.), hydrangea (Macrophylla hydrangea), hibiscus (Hibiscus rosasanensis), roses (Rosa spp.), tulips (Tulipa spp.), daffodils (Narcissus spp.), petunias (Petunia hybrida), carnation (Dianthus caryophyllus), poinsettia (Euphorbia pulcherrima), and chrysanthemum are also included. Additional ornamentals within the scope of the invention include impatiens, Begonia, Pelargonium, Viola, Cyclamen, Verbena, Vinca, Tagetes, Primula, Saint Paulia, Agertum, Amaranthus, Antihirrhinum, Aquilegia, Cineraria, Clover, Cosmo, Cowpea, Dahlia, Datura, Delphinium, Gerbera, Gladiolus, Gloxinia, Hippeastrum, Mesembryanthemum, Salpiglossos, and Zinnia.

[0157] Conifers that may be employed in practicing the present invention include, for example, pines such as loblolly pine (Pinus taeda), slash pine (Pinus elliotii), ponderosa pine (Pinus ponderosa), lodgepole pine (Pinus contorta), and Monterey pine (Pinus radiata), Douglas-fir (Pseudotsuga menziesii); Western hemlock (Tsuga ultilane); Sitka spruce (Picea glauca); redwood (Sequoia sempervirens); true firs such as silver fir (Abies amabilis) and balsam fir (Abies balsamea); and cedars such as Western red cedar (Thuja plicata) and Alaska yellow-cedar (Chamaecyparis nootkatensis).

[0158] Leguminous plants which may be used in the practice of the present invention include beans and peas. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mungbean, lima bean, fava bean, lentils, chickpea, etc. Legumes include, but are not limited to, Arachis, e.g., peanuts, Vicia, e.g., crown vetch, hairy vetch, adzuki bean, mung bean, and chickpea, Lupinus, e.g., lupine, trifolium, Phaseolus, e.g., common bean and lima bean, Pisum, e.g., field bean, Melilotus, e.g., clover, Medicago, e.g., alfalfa, Lotus, e.g., trefoil, lens, e.g., lentil, and false indigo. Preferred forage and turf grass for use in the methods of the invention include alfalfa, orchard grass, tall fescue, perennial ryegrass, creeping bent grass, and redtop.

[0159] Other plants within the scope of the invention include Acacia, aneth, artichoke, arugula, blackberry, canola, cilantro, clementines, escarole, eucalyptus, fennel, grapefruit, honey dew, jicama, kiwifruit, lemon, lime, mushroom, nut, okra, orange, parsley, persimmon, plantain, pomegranate, poplar, radiata pine, radicchio, Southern pine, sweetgum, tangerine, triticale, vine, yams, apple, pear, quince, cherry, apricot, melon, hemp, buckwheat, grape, raspberry, chenopodium, blueberry, nectarine, peach, plum, strawberry, watermelon, eggplant, pepper, cauliflower, Brassica, e.g., broccoli, cabbage, ultilan sprouts, onion, carrot, leek, beet, broad bean, celery, radish, pumpkin, endive, gourd, garlic, snapbean, spinach, squash, turnip, ultilane, chicory, groundnut and zucchini.

[0160] Angiosperms are divided into two broad classes based on the number of cotyledons, which are seed leaves that generally store or absorb food; a monocotyledonous angiosperm has a single cotyledon, and a dicotyledonous angiosperm has two cotyledons. Angiosperms produce a variety of useful products including materials such as lumber, rubber, and paper; fibers such as cotton and linen; herbs and medicines such as quinine and vinblastine; ornamental flowers such as roses and orchids; and foodstuffs such as grains, oils, fruits and vegetables.

[0161] Angiosperms encompass a variety of flowering plants, including, for example, cereal plants, leguminous plants, oilseed plants, hardwood trees, fruit-bearing plants and ornamental flowers, which general classes are not necessarily exclusive. Cereal plants, which produce an edible grain cereal, include, for example, corn, rice, wheat, barley, oat, rye, orchardgrass, guinea grass, sorghum and turfgrass. Leguminous plants include members of the pea family (Fabaceae) and produce a characteristic fruit known as a legume. Examples of leguminous plants include, for example, soybean, pea, chickpea, moth bean, broad bean, kidney bean, lima bean, lentil, cowpea, dry bean, and peanut, as well as alfalfa, birdsfoot trefoil, clover and sainfoin. Oilseed plants, which have seeds that are useful as a source of oil, include soybean, sunflower, rapeseed (canola) and cottonseed.

[0162] Angiosperms also include hardwood trees, which are perennial woody plants that generally have a single stem (trunk). Examples of such trees include alder, ash, aspen, basswood (linden), beech, birch, cherry, cottonwood, elm, eucalyptus, hickory, locust, maple, oak, persimmon, poplar, sycamore, walnut, sequoia, and willow. Trees are useful, for example, as a source of pulp, paper, structural material and fuel.

[0163] Angiosperms are fruit-bearing plants that produce a mature, ripened ovary, which generally contains seeds. A fruit can be suitable for human or animal consumption or for collection of seeds to propagate the species. For example, hops are a member of the mulberry family that are prized for their flavoring in malt liquor. Fruit-bearing angiosperms also include grape, orange, lemon, grapefruit, avocado, date, peach, cherry, olive, plum, coconut, apple and pear trees and blackberry, blueberry, raspberry, strawberry, pineapple, tomato, cucumber and eggplant plants. An ornamental flower is an angiosperm cultivated for its decorative flower. Examples of commercially important ornamental flowers include rose, orchid, lily, tulip and chrysanthemum, snapdragon, camellia, carnation and petunia plants. The skilled artisan will recognize that the methods of the invention can be practiced using these or other angiosperms, as desired, as well as gymnosperms, which do not produce seeds in a fruit.

[0164] A method of producing a transgenic plant can be performed by introducing a polynucleotide portion of plant stress-regulated gene into a plant cell genome, whereby the polynucleotide portion of the plant stress-regulated gene modulates a response of the plant cell to a stress condition, thereby producing a transgenic plant, which comprises plant cells that exhibit altered responsiveness to the stress condition. In one embodiment, the polynucleotide portion of the plant stress-regulated gene encodes a stress-regulated polypeptide or functional peptide portion thereof, wherein expression of the stress-regulated polypeptide or functional peptide portion thereof either increases the stress tolerance of the transgenic plant, or decreases the stress tolerance of the transgenic plant. The polynucleotide portion of the plant stress-regulated gene encoding the stress-regulated polypeptide or functional peptide portion thereof can be operatively linked to a heterologous promoter.

[0165] In another embodiment, the polynucleotide portion of the plant stress-regulated gene comprises a stress-regulated regulatory element. The stress-regulated regulatory element can integrate into the plant cell genome in a site-specific manner, whereupon it can be operatively linked to an endogenous nucleotide sequence, which can be expressed in response to a stress condition specific for the regulatory element; or can be a mutant regulatory element, which is not responsive to the stress condition, whereby upon integrating into the plant cell genome, the mutant regulatory element disrupts an endogenous stress-regulated regulatory element of a plant stress-regulated gene, thereby altering the responsiveness of the plant stress-regulated gene to the stress condition. Accordingly, the invention also provides genetically modified plants, including transgenic plants, produced by such a method, and a plant cell obtained from such genetically modified plant, wherein said plant cell exhibits altered responsiveness to the stress condition; a seed produced by a transgenic plant; and a cDNA library prepared from a transgenic plant.

[0166] Also provided is a method of modulating the responsiveness of a plant cell to a stress condition. Such a method can be performed, for example, by introducing a polynucleotide portion of a plant stress-regulated gene into the plant cell, thereby modulating the responsiveness of the plant cell to a stress condition. As disclosed herein, the responsiveness of the plant cell can be increased or decreased upon exposure to the stress condition, and the altered responsiveness can result in increased or decreased tolerance of the plant cell to a stress condition. The polynucleotide portion of the plant stress-regulated gene can, but need not, be integrated into the genome of the plant cell, thereby modulating the responsiveness of the plant cell to the stress condition. Accordingly, the invention also provide a genetically modified plant, including a transgenic plant, which contains an introduced polynucleotide portion of a plant stress-regulated gene, as well as plant cells, tissues, and the like, which exhibit modulated responsiveness to a stress condition.

[0167] The polynucleotide portion of the plant stress-regulated gene can encode a stress-regulated polypeptide or functional peptide portion thereof, which can be operatively linked to a heterologous promoter. As used herein, reference to a “functional peptide portion of a plant stress-regulated polypeptide” means a contiguous amino acid sequence of the polypeptide that has an activity of the full length polypeptide, or that has an antagonist activity with respect to the fall length polypeptide, or that presents an epitope unique to the polypeptide. Thus, by expressing a functional peptide portion of a plant stress-regulated polypeptide in a plant cell, the peptide can act as an agonist or an antagonist of the polypeptide, thereby modulating the responsiveness of the plant cell to a stress condition.

[0168] A polynucleotide portion of the plant stress-regulated nucleotide sequence also can contain a mutation, whereby upon integrating into the plant cell genome, the polynucleotide disrupts (knocks-out) an endogenous plant stress-regulated nucleotide sequence, thereby modulating the responsiveness of said plant cell to the stress condition. Depending on whether the knocked-out gene encodes an adaptive or a maladaptive stress-regulated polypeptide, the responsiveness of the plant will be modulated accordingly. Thus, a method of the invention provides a means of producing a transgenic plant having a knock-out phenotype of a plant stress-regulated nucleotide sequence.

[0169] Alternatively, the responsiveness of a plant or plant cell to a stress condition can be modulated by use of a suppressor construct containing dominant negative mutation for any of the stress-regulated sequences described herein. Expression of a suppressor construct containing a dominant mutant mutation generates a mutant transcript that, when coexpressed with the wild-type transcript inhibits the action of the wild-type transcript. Methods for the design and use of dominant negative constructs are well known (see, for example, in Herskowitz, Nature 329:219-222, 1987; Lagna and Hemmati-Brivanlou, Curr. Topics Devel. Biol. 36:75-98, 1998).

[0170] The polynucleotide portion of the plant stress-regulated gene also can comprise a stress-regulated regulatory element, which can be operatively linked to a heterologous nucleotide sequence, which, upon expression from the regulatory element in response to a stress condition, modulates the responsiveness of the plant cell to the stress condition. Such a heterologous nucleotide sequence can encode, for example, a stress-inducible transcription factor such as DREB1A, which, upon exposure to the stress condition, is expressed such that it can amplify the stress response (see Kasuga et al., supra, 1999). The heterologous nucleotide sequence also can encode a polynucleotide that is specific for a plant stress-regulated gene, for example, an antisense molecule, a ribozyme, and a triplexing agent, either of which, upon expression in the plant cell, reduces or inhibits expression of a stress-regulated polypeptide encoded by the gene, thereby modulating the responsiveness of the plant cell to a stress condition, for example, an abnormal level of cold, osmotic pressure, and salinity. As used herein, the term “abnormal,” when used in reference to a condition such as temperature, osmotic pressure, salinity, or any other condition that can be a stress condition, means that the condition varies sufficiently from a range generally considered optimum for growth of a plant that the condition results in an induction of a stress response in a plant. Methods of determining whether a stress response has been induced in a plant are disclosed herein or otherwise known in the art.

[0171] A plant stress-regulated regulatory element can be operatively linked to a heterologous polynucleotide sequence, such that the regulatory element can be introduced into a plant genome in a site-specific matter by homologous recombination. For example, a mutant plant stress-regulated regulatory element for a maladaptive stress-induced polypeptide can be transformed into a plant genome in a site specific manner by in vivo mutagenesis, using a hybrid RNA-DNA oligonucleotide (“chimeroplast” (TIBTECH 15:441-447,1997; WO 95/15972; Kren, Hepatology 25:1462-1468, 1997; Cole-Strauss, Science 273:1386-1389, 1996, each of which is incorporated herein by reference). Part of the DNA component of the RNA-DNA oligonucleotide is homologous to a nucleotide sequence comprising the regulatory element of the maladaptive gene, but includes a mutation or contains a heterologous region which is surrounded by the homologous regions. By means of base pairing of the homologous regions of the RNA-DNA oligonucleotide and of the endogenous nucleic acid molecule, followed by a homologous recombination the mutation contained in the DNA component of the RNA-DNA oligonucleotide or the heterologous region can be transferred to the plant genome, resulting in a “mutant” gene that, for example, is not induced in response to a stress and, therefore, does not confer the maladaptive phenotype. Such a method similarly can be used to knock-out the activity of a stress-regulated gene, for example, in an undesirable plant. Such a method can provide the advantage that a desirable wild-type plant need not compete with the undesirable plant, for example, for light, nutrients, or the like.

[0172] A method of modulating the responsiveness of a plant cell to a stress condition also can be performed by introducing a mutation in the chromosomal copy of a plant stress-regulated gene, for example, in the stress-regulated regulatory element, by transforming a cell with a chimeric oligonucleotide composed of a contiguous stretch of RNA and DNA residues in a duplex conformation with double hairpin caps on the ends. An additional feature of the oligonucleotide is the presence of 2′-0-methylation at the RNA residues. The RNA/DNA sequence is designed to align with the sequence of a chromosomal copy of the target regulatory element and to contain the desired nucleotide change (see U.S. Pat. No. 5,501,967, which is incorporated herein by reference).

[0173] A plant stress-regulated regulatory element also can be operatively linked to a heterologous polynucleotide such that, upon expression from the regulatory element in the plant cell, confers a desirable phenotype on the plant cell. For example, the heterologous polynucleotide can encode an aptamer, which can bind to a stress-induced polypeptide. Aptamers are nucleic acid molecules that are selected based on their ability to bind to and inhibit the activity of a protein or metabolite. Aptamers can be obtained by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) method (see U.S. Pat. No. 5,270,163), wherein a candidate mixture of single stranded nucleic acids having regions of randomized sequence is contacted with a target, and those nucleic acids having a specific affinity to the target are partitioned from the remainder of the candidate mixture, and amplified to yield a ligand enriched mixture. After several iterations a nucleic acid molecule (aptamer) having optimal affinity for the target is obtained. For example, such a nucleic acid molecule can be operatively linked to a plant stress-regulated regulatory element and introduced into a plant. Where the aptamer is selected for binding to a polypeptide that normally is expressed from the regulatory element and is involved in an adaptive response of the plant to a stress, the recombinant molecule comprising the aptamer can be useful for inhibiting the activity of the stress-regulated polypeptide, thereby decreasing the tolerance of the plant to the stress condition.

[0174] The invention provides a genetically modified plant, which can be a transgenic plant, that is tolerant or resistant to a stress condition. As used herein, the term “tolerant” or “resistant,” when used in reference to a stress condition of a plant, means that the particular plant, when exposed to a stress condition, shows less of an effect, or no effect, in response to the condition as compared to a corresponding reference plant (naturally occurring wild-type plant or a plant not containing a construct of the present invention). As a consequence, a plant encompassed within the present invention grows better under more widely varying conditions, has higher yields and/or produces more seeds. Thus, a transgenic plant produced according to a method of the invention can demonstrate protection (as compared to a corresponding reference plant) from a delay to complete inhibition of alteration in cellular metabolism, or reduced cell growth or cell death caused by the stress. Preferably, the transgenic plant is capable of substantially normal growth under environmental conditions where the corresponding reference plant shows reduced growth, metabolism or viability, or increased male or female sterility.

[0175] The determination that a plant modified according to a method of the invention has increased resistance to a stress-inducing condition can be made by comparing the treated plant with a control (reference) plant using well known methods. For example, a plant having increased tolerance to saline stress can be identified by growing the plant on a medium such as soil, which contains a higher content of salt in the order of at least about 10% compared to a medium the corresponding reference plant is capable of growing on. Advantageously, a plant treated according to a method of the invention can grow on a medium or soil containing at least about 50%, or more than about 75%, particularly at least about more than 100%, and preferably more than about 200% salt than the medium or soil on which a corresponding reference plant can grow. In particular, such a treated plant can grow on medium or soil containing at least 40 mM, generally at least 100 mM, particularly at least 200 mM, and preferably at least 300 mM salt, including, for example, a water soluble inorganic salt such as sodium sulfate, magnesium sulfate, calcium sulfate, sodium chloride, magnesium chloride, calcium chloride, potassium chloride, or the like; salts of agricultural fertilizers, and salts associated with alkaline or acid soil conditions; particularly NaCl.

[0176] In another embodiment, the invention provides a plant that is less tolerant or less resistant to a stress condition as compared to a corresponding reference plant. As used herein, the term “less tolerant” or “less resistant,” when used in reference to a stress condition of a plant, means that the particular plant, when exposed to a stress condition, shows an alteration in response to the condition as compared to a corresponding reference plant. As a consequence, such a plant, which generally is an undesirable plant species, is less likely to grow when exposed to a stress condition than an untreated plant.

[0177] The present invention also relates to a method of expressing a heterologous nucleotide sequence in a plant cell. Such a method can be performed, for example, by introducing into the plant cell a plant stress-regulated regulatory element operatively linked to the heterologous nucleotide sequence, whereby, upon exposure of the plant cell to stress condition, the heterologous nucleotide sequence is expressed in the plant cell. The heterologous nucleotide sequence can encode a selectable marker, or preferably, a polypeptide that confers a desirable trait upon the plant cell, for example, a polypeptide that improves the nutritional value, digestibility or ornamental value of the plant cell, or a plant comprising the plant cell. Accordingly, the invention provides a transgenic plant that, in response to a stress condition, can produce a heterologous polypeptide from a plant stress-regulated regulatory element. Such transgenic plants can provide the advantage that, when grown in a cold environment for example, expression of the heterologous polypeptide from a plant cold-regulated regulatory element can result in increased nutritional value of the plant.

[0178] The present invention further relates to a method of modulating the activity of a biological pathway in a plant cell, wherein the pathway involves a stress-regulated polypeptide. As used herein, reference to a pathway that “involves” a stress-regulated polypeptide means that the polypeptide is required for normal function of the pathway. For example, plant stress-regulated polypeptides as disclosed herein include those acting as kinases or as transcription factors, which are well known to be involved in signal transduction pathways. As such, a method of the invention provides a means to modulate biological pathways involving plant stress-regulated polypeptides, for example, by altering the expression of the polypeptides in response to a stress condition. Thus, a method of the invention can be performed, for example, by introducing a polynucleotide portion of a plant stress-regulated gene into the plant cell, thereby modulating the activity of the biological pathway.

[0179] A method of the invention can be performed with respect to a pathway involving any of the stress-regulated polypeptides as encoded by a polynucleotide of SEQ ID NOS:1-2703, including for example, a stress-regulated transcription factor, an enzyme, including a kinase, a channel protein (see, for example, Tables 29-31; see, also, Table 1). Pathways in which the disclosed stress-regulated stress factors are involved can be identified, for example, by searching the Munich Information Center for Protein Sequences (MIPS) Arabidopsis thaliana database (MATDB), which is at http://www.mips.biochem.mpg.de/proj/thal/.

[0180] The present invention also relates to a method of identifying a polynucleotide that modulates a stress response in a plant cell. Such a method can be performed, for example, by contacting an array of probes representative of a plant cell genome and nucleic acid molecules expressed in plant cell exposed to the stress; detecting a nucleic acid molecule that is expressed at a level different from a level of expression in the absence of the stress; introducing the nucleic acid molecule that is expressed differently into a plant cell; and detecting a modulated response of the plant cell containing the introduced nucleic acid molecule to a stress, thereby identifying a polynucleotide that modulates a stress response in a plant cell. The contacting is under conditions that allow for selective hybridization of a nucleic acid molecule with probe having sufficient complementarity, for example, under stringent hybridization conditions.

[0181] As used herein, the term “array of probes representative of a plant cell genome” means an organized group of oligonucleotide probes that are linked to a solid support, for example, a microchip or a glass slide, wherein the probes can hybridize specifically and selectively to nucleic acid molecules expressed in a plant cell. Such an array is exemplified herein by a GeneChip® Arabidopsis Genome Array (Affymetrix; see Example 1). In general, an array of probes that is “representative” of a plant genome will identify at least about 30% or the expressed nucleic acid molecules in a plant cell, generally at least about 50% or 70%, particularly at least about 80% or 90%, and preferably will identify all of the expressed nucleic acid molecules. It should be recognized that the greater the representation, the more likely all nucleotide sequences of cluster of stress-regulated genes will be identified.

[0182] A method of the invention is exemplified in Example 1, wherein clusters of Arabidopsis genes induced to cold, to increased salinity, to increased osmotic pressure, and to a combination of the above three stress conditions were identified. Based on the present disclosure, the artisan readily can obtain nucleic acid samples for Arabidopsis plants exposed to other stress conditions, or combinations of stress conditions, and identify clusters of genes induced in response to the stress conditions. Similarly, the method is readily adaptable to identifying clusters of stress-regulated genes expressed in other plant species, particularly commercially valuable plant species, where a substantial amount of information is known regarding the genome.

[0183] The clusters of genes identified herein include those clusters of genes that are induced or repressed in response to a combination of stress conditions, but not to any of the stress conditions alone; and clusters of genes that are induced or repressed in response to a selected stress condition, but not to other stress conditions tested. Furthermore, clusters of genes that respond to a stress condition in a temporally regulated manner are also included, such as gene clusters that are induced early (for example, within about 3 hours), late (for example, after about 8 to 24 hours), or continuously in a stress response. In addition, the genes within a cluster are represented by a variety of cellular proteins, including transcription factors, enzymes such as kinases, channel proteins, and the like (see Tables 1 and 29-31). Thus, the present invention further characterizes nucleotide sequences that previously were known to encode cellular peptides by classifying them within clusters of stress-regulated genes.

[0184] The present invention additionally relates to a method of identifying a stress condition to which a plant cell was exposed. Such a method can be performed, for example, by contacting nucleic acid molecules expressed in the plant cell and an array of probes representative of the plant cell genome; and detecting a profile of expressed nucleic acid molecules characteristic of a stress response, thereby identifying the stress condition to which the plant cell was exposed. The contacting generally is under conditions that allow for selective hybridization of a nucleic acid molecule with probe having sufficient complementarity, for example, under stringent hybridization conditions. The profile can be characteristic of exposure to a single stress condition, for example, an abnormal level of cold, osmotic pressure, or salinity (Tables 3-14), or can be characteristic of exposure to more than one stress condition (Tables 15-26, for example, cold, increased osmotic pressure and increased salinity (see Tables 24-26).

[0185] The method can be practiced using at least one nucleic acid probe and can identify one or combination of stress conditions by detecting altered expression of one or a plurality of polynucleotides representative of plant stress-regulated genes. As used herein, the term “at least one” includes one, two, three or more, for example, five, ten, twenty, fifty or more polynucleotides, nucleic acid probes, and the like. The term “plurality” is used herein to mean two or more, for example, three, four, five or more, including ten, twenty, fifty or more polynucleotides, nucleic acid probes, and the like.

[0186] In a method of the invention, nucleic acid samples from the plant cells to be collected can be contacted with an array, then the profile can be compared with known expression profiles prepared from nucleic acid samples of plants exposed to a known stress condition or combination of stress conditions. By creating a panel of such profiles, representative of various stress conditions, an unknown stress condition to which a plant was exposed can be identified simply by comparing the unknown profile with the known profiles and determining which known profile that matches the unknown profile. Preferably, the comparison is automated. Such a method can be useful, for example, to identify a cause of damage to a crop, where the condition causing the stress is not known or gradually increases over time. For example, accumulation in soils over time of salts from irrigation water can result in gradually decreasing crop yields. Because the accumulation is gradual, the cause of the decreased yield may not be readily apparent. Using the present methods, it is possible to evaluate the stress to which the plants are exposed, thus revealing the cause of the decreased yields.

[0187] The present invention, therefore includes a computer readable medium containing executable instructions form receiving expression data for sequences substantially similar to any of those disclosed herein and comparing expression data from a test plant to a reference plant that has been exposed to an abiotic stress. Also provided is a computer-readable medium containing sequence data for sequences substantially similar to any of the sequences described herein, or the complements thereof, and a module for comparing such sequences to other nucleic acid sequences.

[0188] Also provided are plants and plant cells comprising plant stress-regulatory elements of the present invention operably linked to a nucleotide sequence encoding a detectable signal. Such plants can be used as diagnostic or “sentinel” plants to provide early warning that nearby plants are being stressed so that appropriate actions can be taken. In one embodiment, the signal is one that alters the appearance of the plant. For example, an osmotic stress regulatory element of the present invention can be operably linked to a nucleotide sequence encoding a fluorescent protein such as green fluorescent protein. When subjected to osmotic stress, the expression of the green fluorescent protein in the sentinel plant provides a visible signal so that appropriate actions can be taken to remove or alleviate the stress. The use of fluorescent proteins in plants is well known (see, for example, in Leffel et al., BioTechniques 23:912, 1997).

[0189] The invention further relates to a method of identifying an agent that modulates the activity of a stress-regulated regulatory element of a plant. As used herein, the term “modulate the activity,” when used in reference to a plant stress-regulated regulatory element, means that expression of a polynucleotide from the regulatory element is increased or decreased. In particular, expression can be increased or decreased with respect to the basal activity of the promoter, i.e., the level of expression, if any, in the absence of a stress condition that normally induces expression from the regulatory element; or can be increased or decreased with respect to the level of expression in the presence of the inducing stress condition. As such, an agent can act as a mimic of a stress condition, or can act to modulate the response to a stress condition.

[0190] Such a method can be performed, for example, by contacting the regulatory element with an agent suspected of having the ability to modulate the activity of the regulatory element, and detecting a change in the activity of the regulatory element. In one embodiment, the regulatory element can be operatively linked to a heterologous polynucleotide encoding a reporter molecule, and an agent that modulates the activity of the stress-regulated regulatory element can be identified by detecting a change in expression of the reporter molecule due to contacting the regulatory element with the agent. Such a method can be performed in vitro in a plant cell-free system, or in a plant cell in culture or in a plant in situ.

[0191] A method of the invention also can be performed by contacting the agent is contacted with a genetically modified cell or a transgenic plant containing an introduced plant stress-regulated regulatory element, and an agent that modulates the activity of the regulatory element is identified by detecting a phenotypic change in the modified cell or transgenic plant.

[0192] A method of the invention can be performed in the presence or absence of the stress condition to which the particularly regulatory element is responsive. As such, the method can identify an agent that modulates the activity of plant stress-regulated promoter in response to the stress, for example, an agent that can enhance the stress response or can reduce the stress response. In particular, a method of the invention can identify an agent that selectively activates the stress-regulated regulatory elements of a cluster of plant stress-regulated genes, but does not affect the activity of other stress-regulated regulatory genes. As such, the method provides a means to identify an agent that acts as a stress mimic. Such agents can be particularly useful to prepare a plant to an expected stress condition. For example, a agent that acts as a cold mimic can be applied to a field of plants prior to the arrival of an expected cold front. Thus, the cold stress response can be induced prior to the actual cold weather, thereby providing the plants with the protection of the stress response, without the plants suffering from any initial damage due to the cold. Similarly, an osmotic pressure mimic can be applied to a crop of plants prior a field being flooded by a rising river.

[0193] In one embodiment, the present invention provides a method for marker-assisted selection. Marker-assisted selection involves the selection of plants having desirable phenotypes based on the presence of particular nucleotide sequences (“markers”). The use of markers allows plants to be selected early in development, often before the phenotype would normally be manifest. Because it allows for early selection, marker-assisted selection decreases the amount of time need for selection and thus allows more rapid genetic progress. Briefly, marker-assisted selection involves obtaining nucleic acid from a plant to be selected. The nucleic acid obtained is then probed with probes that selectively hybridize under stringent, preferably highly stringent, conditions to a nucleotide sequence or sequences associated with the desired phenotype. In one embodiment, the probes hybridize to any of the stress-responsive genes or regulatory regions disclosed herein, for example, any one of SEQ ID NOS:1-2703. The presence of any hybridization products formed is detected and plants are then selected on the presence or absence of the hybridization products.

[0194] The following examples are intended to illustrate but not limit the invention.

EXAMPLE 1

Profiling of Plant Stress-Regulated Genes

[0195] This example demonstrates that clusters of stress-regulated genes can be identified in plant cells exposed to various stress conditions, either alone or in combination.

[0196] A GeneChip® Arabidopsis Genome Array (Affymetrix, Santa Clara, Calif.) was used to identify clusters of genes that were coordinately induced in response to various stress conditions. The GeneChip® Arabidopsis Genome Array contains probes synthesized in situ and is designed to measure temporal and spatial gene expression of approximately 8700 genes in greater than 100 EST clusters. The sequences used to develop the array were obtained from GenBank (http://www.ncbi.nlm.nih.gov/) in collaboration with Torrey Mesa Research Institute (San Diego, Calif.), formerly known as Novartis Agriculture Discovery Institute. Eighty percent of the nucleotide sequences represented on the array are predicted coding sequences from genomic BAC entries; twenty percent are high quality cDNA sequences. The array also contains over 100 EST clusters that share homology with the predicted coding sequences from BAC clones (see, for example, world wide web at address (url) “affymetrix.com/products/Arabidopsis_content.html”.

[0197] The Affymetrix GeneChip® array was used to define nucleotide sequences/pathways affected by various abiotic stresses and to define which are uniquely regulated by one stress and those that respond to multiple stress, and to identify candidate nucleotide sequences for screening for insertional mutants. Of the approximately 8,700 nucleotide sequences represented on the Affymetrix GeneChip® array, 2862 nucleotide sequences showed at least a 2-fold change in expression in at least one sample, relative to no-treatment controls. Of those 2,862 nucleotide sequences 1,335 were regulated only by cold stress, 166 were regulated only mannitol stress and 209 were regulated only by saline stress. Furthermore, of the 2,862 nucleotide sequences 123 nucleotide sequences were regulated by salt and mannitol stress, 293 were regulated by mannitol and cold stress, 274 were regulated by cold and saline stress and 462 were regulated by cold, mannitol and salt. Of the 2,862 nucleotide sequences, 771 passed the higher stringency of showing at least a 2-fold change in expression in at least 2 samples, relative to control. And, 508 of the 771 nucleotide sequences were found in an in-house collection of insertion mutants.

[0198] The following describes in more detail how the experiments were done. Transcriptional profiling was performed by hybridizing fluorescence labeled cRNA with the oligonucleotides probes on the chip, washing, and scanning. Each gene is represented on the chip by about sixteen oligonucleotides (25-mers). Expression level is related to fluorescence intensity. Starting material contained 1 to 10 μg total RNA; detection specificity was about 1:106; approximately a 2-fold change was detectable, with less than 2% false positive; the dynamic range was approximately 500×. Nucleotide sequences having up to 70% to 80% identity could be discriminated using this system.

[0199] Seven day old axenic Arabidopsis seedlings were transferred to Magenta boxes with rafts floating on MS medium. Three weeks later (28 day old seedlings), stresses were applied as follows: Control−no treatment; Cold−Magenta box placed in ice; Mannitol−medium+200 mM mannitol; Salt−medium+100 mM NaCl. Tissue samples were collected at 3 hours and 27 hours into the stress, roots and aerial portions were harvested, RNA was purified, and the samples were analyzed using the GeneChip® Arabidopsis Genome Array (Affymetrix, Santa Clara, Calif.) following the manufacturer's protocol.

[0200] Raw fluorescence values as generated by Affymetrix software were processed as follows: the values were brought into Microsoft Excel and values of 25 or less were set to 25 (an empirically determined baseline, Zhu and Wang, Plant Physiol. 124:1472-1476; 2000). The values from the stressed samples were then converted to fold change relative to control by dividing the values from the stressed samples by the values from the no-treatment control samples. Expression patterns that were altered at least 2-fold with respect to the control were selected. This method gave very robust results and resulted in a larger number of nucleotide sequences called as stress-regulated than previous methods had permitted.

[0201] Based on the profiles obtained following hybridization of nucleic acid molecules obtained from plant cells exposed to various stress conditions to the probes in the microarray, clusters of nucleotide sequences that were altered in response to the stress conditions were identified (see Tables 3-6, cold responsive; Tables 7-10, salt (saline) responsive; Tables 11 to 14, mannitol (osmotic) responsive; Tables 15-17, cold and mannitol responsive; Tables 18-20, 6 salt and cold responsive; Tables 21-23, salt and mannitol responsive; Tables 24-26, cold, salt and mannitol responsive. Examples of plant gene sequences that varied in expression at least two-fold in response to a combination of cold, saline and osmotic stress in root cells and leaf cells are shown in Tables 27 and 28, respectively. In addition, examples of plant gene sequences that encode transcription factors (Table 29), phosphatases (Table 30), and kinases (Table 31) and that varied at least two-fold in response to a combination of cold, saline and osmotic stress are provided.

[0202] Affymetrix ID numbers and corresponding SEQ ID NOS: for the respective Arabidopsis nucleotide sequences are provided Tables 3-26, and can be used to determine SEQ ID NOS: for the sequences shown by Affymetrix ID number in Tables 27-31. The Affymetrix ID number refers to a particular nucleotide sequence on the GeneChip® Arabidopsis Genome Array. In some cases, a particular plant stress-regulated gene sequence hybridized to more than one nucleotide sequence on the GeneChip® Arabidopsis Genome Array (see, for example, Table 3, where SEQ ID NO:36 is shown to have hybridized to the 12187_AT and 15920_I_AT nucleotide sequences on the GeneChip®). In addition, it should be recognized that the disclosed sequences are not limited to coding sequences but, in some cases, include 5′ untranslated sequences (see Table 2) or a longest coding region. As such, while the sequences set forth as SEQ ID NOS:1-2073 generally start with an ATG codon, in most cases each comprises a longer nucleotide sequence, including a regulatory region (see Table 2).

[0203] The results disclosed herein demonstrate that several polynucleotides, some of which were known to function as transcription factors, enzymes, and structural proteins, also are involved in the response of a plant cell to stress. The identification of the clusters of stress-regulated genes as disclosed herein provides a means to identify stress-regulated regulatory elements present in Arabidopsis thaliana nucleotide sequences, including consensus regulatory elements. It should be recognized, however that the regulatory elements of the plant genes comprising a sequence as set forth in SEQ ID NOS:156, 229, 233, 558, 573, 606, 625, 635, 787, and 813, which previously have been described as cold regulated genes, are not encompassed within the stress-regulated gene regulatory element of the invention, and the regulatory elements of the plant genes comprising the nucleotide sequences set forthas SEQ ID NOS:1263, 1386, 1391, 1405, 1445, 1484, 1589, 1609, 1634, 1726, 1866, 1918, and 1928, which previously have been identified as genes that are responsive to a single stress condition such as cold or saline stress, are not encompassed within the plant stress-regulated gene regulatory elements of the invention to the extent that they confer stress-regulated expression only with respect to the known single stress. Furthermore, the identification of the Arabidopsis stress-regulated genes provides a means to identify the corresponding homologs and orthologs in other plants, including commercially valuable food crops such as wheat, rice, soy, and barley, and ornamental plants. BLASTN and BLASTP searches to identify such sequences revealed the polynucleotide sequences set forth in Table 32, which is on the CD-R compact disc submitted herewith.

[0204] Although the invention has been described with reference to the above example, it will be understood that modifications and variations are encompassed within the spirit and scope of the invention. Accordingly, the invention is limited only by the claims, which follow Tables 1 to 31.

1TABLE 1SEQUENCE DESCRIPTIONSSeqIDDescription1unknown protein2unknown protein3unknown protein4putative auxin-inducedprotein5unknown protein6hypothetical protein7putative protein8unknown protein9unknown protein10unknown protein11putative protein12Thioredoxin - like protein13putative RNA helicase14putative protein15putative protein16RING zinc finger protein,putative17putative cyclin18putative protein19putative protein20unknown protein21putative protein22putative protein23hypothetical protein24unknown protein25hypothetical protein26unknown protein27unknown protein28unknown protein29unknown protein30putative protein31putative protein32putative protein33unknown protein34putative ribonuclease III35unknown protein36unknown protein37unknown protein38unknown protein39unknown protein40putative histidine kinase41scarecrow-like 7 (SCL7)42putative protein43No function assigned by TJGR44unknown protein45unknown protein46succinyl-CoA-ligase alpha subunit47putative protein48CLV1 receptor kinase like protein49putative receptor-like proteinkinase50putative squalene synthase51putative receptor protein kinase52somatic embryo genesis receptor-like kinase, putative53putative protein54putative beta-glucosidase55multi-drug resistance protein56receptor protein kinase (TMK1),putative57putative receptor-like proteinkinase58putative pectate lyase59putative protein kinase60putative peroxidase61cytochrome P450-like protein62putative beta-amylase63monosaccharide transporter STP364Lycopersicon esculentumproteinase TMP, Pir2:T0761765putative receptor-like proteinkinase66G-box-binding factor 167amino acid carrier, putative68myb-related protein69No function assigned by TIGR70SNF1 like protein kinase71Cu/Zn superoxide dismutase-likeprotein72putative protein kinase73small nuclear ribonucleoproteinU1A74ras-like GTP-bindingprotein75oleoyl-[acyl- carrier-protein]hydrolase-like protein 76putative heat shocktranscription factor77putative protein78membrane-bound smallGTP-binding - like protein79putative protein (fragment)80indole-3-acetate beta-glucosyltransferase likeprotein81HD-zip transcription factor(athb-8)82putative cAMP-dependentprotein kinase83glucuronosyl transferase-like protein84putative leucine-rich repeatdisease resistance protein8598b like protein86putative receptor-likeprotein kinase87IAA-Ala hydrolase (IAR3)88putative AP2 domaintranscription factor89putative expansin90putative Ap2 domainprotein91expansin (At-EXP1)92cytochrome P450 - likeprotein93putative ATP-dependentRNA helicase A94unknown protein95predicted protein96putative glucosyltransferase97unknown protein98putative xyloglucan-specific glucanase99cysteine synthase100clathrin assembly proteinAP19 homolog101dynein light chain like protein102chaperonin CPN10103putative bHLH transcription factorputative glyoxysomal malatedehydrogenase precursor105ATP-dependent RNA helicase,putative106chlorophyll synthetase107similar to epoxide hydrolases108putative protein109unknown protein110hypothetical protein111putative membrane transporter112putative tyrosyl-tRNA synthetase113ARGININE/SERINE-RICHSPLICING FACTOR RSP31114putative oxidoreductase115unknown protein116linker histone protein, putative117hypothetical protein118putative protein119putative mitochondrial carrierprotein120putative transcription factor121MYB-related protein122myb-related transcription factor,putative123unknown protein124unknown protein125putative glycine-rich protein126No function assigned by TIGR127unknown protein128unknown protein129unknown protein130unknown protein131putative membrane channel protein132putative protein133unknown protein134gamma glutamyl hydrolase,putative13540S ribosomal protein S5136DnaJ-like protein13740S ribosomal protein S26138putative WRKY-type DNA bindingprotein139putative protein140hypothetical protein141putative ubiquitin-conjugating enzyme142peptidyiprolyl isomeraseROC1143glyceraldehyde-3-phosphate dehydrogenase Csubunit (GapC)144No function assigned byTIGR145putative protein146putative thioredoxin147thioredoxin h, putative148thioredoxin-like149allene oxide synthase(emb|CAA73184.1)150anthranilate synthasecomponent I-1 precursor(sp|P32068)151CELL DIVISIONCONTROL PROTEIN 2HOMOLOG A152protein kinase cdc2homolog B153ethylene responsiveelement binding factor 1(frameshift!)154ethylene responsiveelement binding factor 2(ATERF2) (sp|O80338)155ethylene responsiveelement binding factor 5(ATERF5) (sp|O80341)156glucose-6-phosphatedehydrogenase157photomorphogenesisrepressor (COP1)158unknown protein159DNA (cytosine-5)-methyltransferase (DNAmethyltransferase) (DNAmetase) (sp|P34881)160PROLIFERA161putative photomorphogenesisrepressor protein162SNF1-like protein kinase (AKin11)163thioredoxin h164thioredoxin165Ca2+-dependent lipid-bindingprotein, putative166putative auxin-induced protein167putative bZIP transcription factor168hypothetical protein169putative AVR9 elicitor responseprotein170putative serine/threonine proteinkinase171bZIP transcription factor ATB2172putative spliceo some associatedprotein1733-hydroxyisobutyryl-coenzyme Ahydrolase - like protein174putative protein175putative Mutator-like transposase176putative protein177unknown protein178putative protein179putative protein180putative galactinol synthase181putative transcriptional regulator182nuclear matrix constituent protein 1(NMCP 1)-like183putative DNA-binding proteinRAV2184No function assigned by TIGR185basic blue protein, 5′ partial186unknown protein187putative calcium-binding protein,calreticulin188putative pyrophosphate-fructose-6-phosphate 1 -phosphotransferase189ribosomal protein L11, cytosolic190putative dTDP-glucose 4-6-dehydratase19140S ribosomal protein S20-likeprotein19260S ribosomal protein L24193coatomer-like protein, epsilon subunit194glycoprotein(EP1), putative 195putative SPL1-relatedprotein196unknown protein197putative transport proteinSEC61 beta-subunit198unknown protein199putative cytochrome P450200UTP-glucoseglucosyltransferase - likeprotein201605 ribosomal protein L2320240S ribosomal protein 517203405 ribosomal protein S26204protein translation factorSui1 homolog, putative205unknown protein206gamma glutamyl hydrolase,putative207dTDP-glucose 4,6-dehydratase, putative208extensin - like protein209unknown protein210protein phosphatase 2C - like protein211ubiquitin-like protein212protein phosphatase 2C-likeprotein213unknown protein214putative RING zinc fingerankyrin protein215unknown protein216putative rubisco subunit binding-protein alphasubunit217putative acetone-cyanohydrin lyase218putative isoamylase219putative protein220HSP associated protein like22160S ribosomal protein L39222unknown protein223putative SF16 protein {Helianthusannuus}224unknown protein225thioredoxin226trehalose-6-phosphate phosphatase(AtTPPB)227chlorophyll a/b-binding protein228class IV chitinase (CHIV)229chalcone synthase (naringenin-chalcone synthase) (testa 4 protein)(sp|P13114)230unknown protein231cinnamyl-alcohol dehydrogenaseELI3-2232famesyl-pyrophosphate synthetaseFPS2233phospholipid hydroperoxideglutathione peroxidase234heat shock transcription factorHSF4235heat shock protein 10123617.6 kDa heat shock protein (AA1-156)237heat shock protein 17.6A238heat-shock protein239HY5240putative auxin-induced protein,IAA12241early auxin-induced protein,IAA19242auxin-inducible gene (IAA2)243putative protein244putative choline kinase245thymidylate kinase - like protein246CTP synthase like protein247putative protein248putative amidase2494-alpha-glucanotransferase250hypothetical protein251similar to auxin-induced protein252putative protein253putative protein254putative protein255hyuC-like protein256putative tetracyclinetransporter protein257similar to early nodulins258putative protein259putative peptidyl-prolyl cis- trans isomerase260unknown protein261unknown protein262putative endochitinase263putative ABC transporter264No function assigned byTIGR265CONSTANS-like B-boxzinc finger protein266unknown protein267unknown protein268putative mitochondrialprocessing peptidase alphasubunit269putative pre-mRNAsplicing factor270putative phosphatidylserinedecarboxylase271unknown protein272unknown protein273unknown protein274putative casein kinase I275unknown protein27660S ribosomal proteinL23A277putative mitochondrialdicarboxylate carrierprotein278enoyl-ACP reductase (enr-A)279putative isoamylase280formamidase - like protein281reticuline oxidase - likeprotein282unknown protein283putative transketolaseprecursor284putative protein285unknown protein286unknown protein287unknown protein288putative esterase D289predicted protein of unknownfunction290unknown protein291putative indole-3-glycerolphosphate synthase292isopentenylpyrophosphate:dimethyllallylpyrophosphate isomerase293kinase associated proteinphosphatase294putative K+ chaunel, beta subunit295KNAT1 homeobox-like protein296PSI type II chlorophyll a/b-bindingprotein, putative297transcription factor298putative WD-40 repeat protein,MSI2299WD-40 repeat protein (MSI3)300putative WD-40 repeat protein,MSI4301unknown protein302hypothetical protein303putative protein304No function assigned by TIGR305polyphosphoinositide bindingprotein, putative306hypothetical protein307unknown protein308chioroplast ribosomal L1 - likeprotein309cold-regulated protein cor15bprecursor310cyanohydrin lyase like protein311putative replication protein A1312putative protein313possible apospory-associated likeprotein314DNA binding protein GT-1,putative315AT-hook DNA-binding protein(AHP1)316putative phospholipase317chloroplast FtsH protease, putative318enoyl-CoA hydratase like protein319berberine bridge enzyme -like protein320putative sugar transporter321unknown protein322No function assigned byTIGR323hypothetical protein324putative acidic ribosomalprotein325putative protein326unknown protein327hypothetical protein328putative protein329dihydroxypolyprenyl-benzoate methyltransferase 330unknown protein331myb-related protein332No function assigned byTIGR333putative protein334putative disease resistanceresponse protein335hypothetical protein336No function assigned byTIGR337starch branching enzyme II338No function assigned byTIGR339putative enolase (2-phospho-D-glyceratehydroylase)340putative protein kinase341HD-Zip protein, putative342putative protein kinase343phenylalanyl-trnasynthetase - like protein344putative aconitase345NAM(no apical meristem)protein, putative346unknown protein347putativephosphomannomutase348putative famesylated protein349unknown protein350water stress-induced protein,putative351unknown protein352unknown protein353PEROXISOMAL MEMBRANEPROTEIN PMP22354putative peroxisomal membranecarrier protein355putative protein356unknown protein357putative protein358putative protein359argininosuccinate synthase -likeprotein3601-phosphatidylinositol-4,5-bisphosphate phosphodiesterase361putative JUN kinase activatorprotein362putative 60S ribosomal protein L35363nucleoid DNA-binding proteincnd41 - like protein364SigA binding protein365hypothetical protein366putative protein kinase367unknown protein368regulatory protein NPR1-like;transcription factor inhibitor Ikappa B-like369putative protein370hypothetical protein371phosphoribosylanthranilateisomerase372phosphoribosylanthranilateisomerase373sterol glucosyltransferase, putative374putative gigantea protein375putative MYB family transcriptionfactor376hypothetical protein377hypothetical protein378predicted protein379cytochrome P450, putative380putative Na+ dependentileal bile acid transporter381unknown protein382RING-H2 finger proteinRHF1 a383putative protein384unknown protein385putative protein386putative auxin-regulatedprotein387hypothetical protein388unknown protein389unknown protein390putative protein391putative protein392unknown protein393histone H1394Argonaute (AGO1)-likeprotein395unknown protein396putative protein with C-terminal RING finger397unknown protein398unknown protein399unknown protein400unknown protein401unknown protein402putative copper amineoxidase403unknown protein404unknown protein405unknown protein406putative protein407putative protein408unknown protein409unknown protein410putative protein411putative protein412unknown protein413serine/threonine kinase -like protein414alcohol dehydrogenase,putative415anthranilatephosphoribosyltransferase,chloroplast precursor (sp|Q02166)416phytochrome C(sp|P14714)417putative phytochrome-associatedprotein 3418receptor serine/threonine kinasePR5K419Ran-binding protein (atranbp1a)420small Ras-like GTP-bindingprotein (gb|AAB58478.1)421sterol-C5-desaturase422tryptophan synthase beta chain 1precursor (sp|P14671)423thioredoxin f2 (gb|AAD35004.1)424No function assigned by TIGR425putative WRKY DNA-bindingprotein426putative protein427unknown protein428unknown protein42914-3-3 protein homolog RCI1(pin||S47969)430unknown protein431putative CCCH-type zinc fingerprotein432PINHEAD (gb|AAD40098.1);translation initiation factor433plasma membrane proton ATPase(PMA)434CHLOROPHYLL A-B BINDINGPROTEIN 4 PRECURSORhomolog435membrane related protein CP5,putative436ABC transporter (AtMRP2)437putative embryo-abundant protein438putative anthocyanidin-3-glucosiderhamnosyltransferase439putative lipid transfer protein440unknown protein441unknown protein442galactinol synthase, putative443putative protein444putative protein445SCARECROW-like protein446unknown protein447unknown protein448unknown protein449unknown protein450asparagine--tRNA ligase451putative protein452glutamate-1-semialdehyde2,1-aminomutase 1precursor (GSA 1)(glutamate-1-semialdehydeaminotransferase 1) (GSA-AT 1) (sp|P42799)453hypothetical protein454putative serine protease-likeprotein455No function assigned byTIGR456unknown protein457unknown protein458gamma-adaptin, putative459UDP rhamnose--anthocyanidin-3-glucosiderhamnosyltransferase - likeprotein460carbonate dehydratase - likeprotein461putative microtubule-associated protein462putative ribophorin I463putative zinc finger protein464chioroplast FtsH protease,putative465putative protein466unknown protein467putative LEA protein468putative protein469putative protein470unknown protein471putative purple acidphosphatase472unknown protein473putative protein474unknown protein475chlorophyll binding protein,putative476phosphoenolpyruvate carboxylase(PPC)477chlorophyll a/b-binding protein -like478AtAGP4479putative cryptochrome 2 apoprotein480type 2 peroxiredoxin, putative481Atpm24.1 glutathione S transferase482delta tonoplast integral protein(delta-TIP)483205 proteasome subunit (PAA2)484dormancy-associated protein,putative485putative cytidine deaminase486No function assigned by TIGR487putative phospholipase D-gamma488cell elongation protein, Dwarf1489germin-like protein490hevein-like protein precursor (PR-4)491rac-like GTP binding protein(ARAC5)492phosphoprotein phosphatase, type1 catalytic subunit493ubiquitin-protein ligase UBC9494xyloglucan endotransglycosylase-related protein XTR-7495cysteine synthase496putative villin 2497glutathione 5-transferase4985-adenylylsulfate reductase499arginine decarboxylase500ATHP2, putative501ornithine carbamoyltransferaseprecursor502puative protein503putative protein504unknown protein505putative protein506putative protein507unknown protein508unknown protein509unknown protein510unknown protein511hypothetical protein512putative protein513putative DnaJ protein514plastocyanin515unknown protein516unknown protein517unknown protein518unknown protein519unknown protein520unknown protein521putative ATP-dependentRNA helicase522non-race specific diseaseresistance protein (NDR1)523hypothetical protein524putative protein525putative protein526putative protein527copper transport protein528putative protein529unknown protein530unknown protein531unknown protein532putative protein kinase533unknown protein534putative protein535putative protein536hypothetical protein537putative protein538putative AP2 domaintranscription factor539putative nitrilase540putative protein541putative tetrahydrofolatesynthase542heat-shock protein543unkown protein544unknown protein545histone H4546hypothetical protein547unknown protein548putative protein549predicted protein550putative dihydrolipoamidesuccinyltransferase551actin3552putative CCCH-type zinc fingerprotein553MAP kinase kinase 2554ethylene-insensitive3-like1 (EIL1)555histidine transport protein (PTR2-B)556putative auxin-induced proteinAUX2-11557hydroxyacyiglutathione hydrolasecytoplasmic (glyoxalase II) (GLXII)558delta-8 sphingolipid desaturase559cellulose synthase catalytic subunit(Ath-A)560nitrate transporter (NTL1)561DNA-binding homeotic proteinAthb-2562hypothetical protein563aspartate aminotransferase5644-coumarate:CoA ligase 1565pyruvate dehydrogenase E1 betasubunit, putative566nucleotide diphosphate kinase Ia(emb|CAB58230.1)567chloroplast Cpn21 protein568ATP dependent copper transporter569very-long-chain fatty acidcondensing enzyme (CUT1)570putative purine-rich single-strandedDNA-binding protein571serine/threonine proteinphosphatase (type 2A)572isopentenyldiphosphate:dimethylallyldiphosphate isomerase (IPP2)573putative c2h2 zinc fingertranscription factor574putative 20S proteasome betasubunit PBC2575nucleoside diphosphate kinase 3(ndpk3)576ras-related small GTP-bindingprotein577putative 4-coumarate:CoA ligase 2578transcription factor HBP-1bhomolog (sp|P43273)579biotin synthase (Bio B)580homeobox protein HAT22581putative preproteintranslocase SECY protein582carbamoylphosphatesynthetase, putative583putative protein kinase,ADK1584putative nuclear DNA-binding protein G2p585hypothetical protein586hypothetical protein587unknown protein588unknown protein589molybdopterin synthase(CNX2)590putative ribosomal proteinL6591unknown protein592En/Spm-like transposonprotein593putative protein594putative protein595unknown protein596hypothetical protein597unknown protein598unknown protein599putative lysosomal acidlipase600unknown protein601unknown protein602NifS-like aminotranfserase603actin 8604hypothetical protein605putative protein606heat-shock protein (At-hsc70-3)607putative protein disulfideisomerase precursor608adenosine nucleotidetranslocator609photosystem II oxygen-evolvingcomplex protein 3 - like610sedoheptulose-bisphosphataseprecursor611glutathione 5-transferase (GST6)612geranylgeranyl reductase613hypothetical protein614hypothetical protein615phosphoribulokinase precursor616high mobility group protein(HMG1), putative617protease inhibitor II618protease inhibitor II619cytochrome P450 90A1(sp|Q42569)620unknown protein621heat shock protein 90622tubulin beta-9 chain623putative ubiquitin carboxylterminal hydrolase624protein kinase625DRE/CRT-binding proteinDREB1C626histidyl-tRNA synthetase627splicing factor, putative628glutamyl-tRNA synthetase629putative RING zinc finger protein630phytochelatin synthase(gb|AAD41794.1)631putative C2H2-type zinc fingerprotein632putative ligand-gated ion channelprotein633putative ribosomal-protein S6kinase (ATPK6)634MOLYBDOPTERINBIOSYNTHESIS CNX1PROTEIN635temperature-sensitive omega-3fatty acid desaturase, chloroplastprecursor (sp|P48622)636adenylosuccinate synthetase637putative 14-3-3 protein638putative cytochrome P450639putative two-component response regulator 3 protein640putative RING-H2 zincfinger protein ATL6641No function assigned byTIGR642small zinc finger-likeprotein643hypothetical protein644MAP kinase (ATMPK6)645vacuolar ATP synthase,putative646kinesin-like protein647serine/threonine-specificprotein kinase NAK648No function assigned by678TIGR649ACTTN 2/7 (sp|P53492)650phosphoglycerate kinase,putative651homeotic protein BEL1homolog652proline iminopeptidase653pasticcino 1654serine/threonine proteinkinase655cytochrome P450monooxygenase(CYP71B4)656No function assigned byTIGR657putative GDSL-motiflipase/hydrolase658putative protein659unknown protein660hypothetical protein661putative glycosylationenzyme662No function assigned byTIGR663No function assigned byTIGR664unknown protein665putative ABC transporter666nifU-like protein667putative receptor-like proteinkinase668putative disease resistance protein669receptor-like protein kinase - like670ubiquitin activating enzyme 2(gb|AAB37569.1)671No function assigned by TIGR672putative receptor-like proteinkinase673K+ transporter, AKT1674shaggy-like kinase beta675heat shock protein 70676plasma membrane intrinsic protein1a677HSP9O-like proteinhistone H1, putative679unknown protein680dnaK-type molecular chaperonehsc70.1 - like681gamma- glutamylcysteinesynthetase682peroxidase (ATP22a)683putative serine carboxypeptidaseprecursor684putative dioxygenase685glucose transporter686NOI protein, nitrate-induced687putative protein688putative protein689unknown protein690putative photosystem I reactioncenter subunit II precursor691putative protein692unknown protein693cobalamin biosynthesis protein694adenine nucleotide translocase695glutathione transferase, putative696putative 60S ribosomal protein L21697cytochrome P450 like protein698cytochrome b245 beta chainhomolog RbohAp108, putative699RNA helicase, DRH1700putative aldolase701farnesyltransferase subunit A(FTA)702No function assigned by TIGR703putative putative sister-chromatide cohesionprotein704calcium-dependent proteinkinase705serine/threonine protein phosphatase type 2A,putative70640S ribosomal protein S28(sp|P34789)707RNA polymerase subunit708DNA-damage-repair/toleration proteinDRT102709putative C2H2-type zincfinger protein710putative adeno sinephosphosulfate kinase711lipase712putative violaxanthin de-epoxidase precursor(U44133)713aromatic rich glycoprotein,putative714putative fumarase715flavonol synthase (FLS)(sp|Q96330)716response regulator 5,putative717sulfate transporter718putative floral homeoticprotein, AGL9719putative ethylene-inducibleprotein720C-8,7 sterol isomerase721TCH4 protein(gb|AAA92363.1)722hypothetical protein723putative urease accessoryprotein724molybdopterin synthasesulphurylase(gb|AAD18050.1)725putative protein726NBD-like protein(gb|AAD20643.1)727AtHVA22c728unknown protein729phytoene synthase(gb|AAB65697.1)730protein kinase (AME2/AFC1)731hypothetical protein732cyclin-dependent protein kinase-like protein733photosystem II stability/assemblyfactor HCF136 (sp|O82660)734hypothetical protein735DNA binding-like protein736putative protein737chorismate mutase738putative LRR receptor proteinkinase739putative chalcone synthase740putative protein kinase741replicase, putative742putative cysteine proteinase74360S ribosomal protein L36744unknown protein745CLC-b chloride channel protein746putative ribosomal protein S14747histone H2B like protein(emb|CAA69025.1)74860S ribosomal protein L274960S ribosomal protein L15homolog750ribosomal protein S27751ribosomal protein75260S ribosomal protein L1275360S ribosomal protein L34754putative ribosomal protein S10755drought-induced protein like756blue copper-binding protein, 15K(lamin)757calmodulin-like protein758putative protein759No function assigned by TIGR760alpha-mannosidase, putative761uncoupling protein (ucp/PUMP)762homeodomain - like protein763ribosomal protein S18,putative764similar to SOR1 from thefungus Cercosporanicotianae76560S ribosomal protein L13,BBC1 protein76650S ribosomal protein L24,chloroplast precursor767putative ribosomal protein768unknown protein769aspartate aminotransferase(AAT1)770potassium channel proteinAtKC771unknown protein772peroxisomal targetingsignal type 2 receptor773putative protein774Ras-related GTP-bindingprotein (ARA-4)775S-receptor kinase homolog2 precursor776pathogenesis-related group5 protein, putative777Nitrilase 4 (sp|P46011)778biotin carboxyl carrierprotein of acetyl-CoAcarboxylase precursor(BCCP) (sp|Q42533)779photosystem I reactioncentre subunit psaNprecursor (PSI-N)(sp|P49107)7803(2),5-bisphosphatenucleotidase781high affinity Ca2+antiporter782putative cytoskeletalprotein783putative peroxidase784respiratory burst oxidaseprotein785beta-glucosidase786calcium-dependent protein kinase(pir||571196)787phosphoinositide specificphospholipase C788similarity to S-domain receptor-like protein kinase, Zea mays789mitosis-specific cyclin 1b7904-coumarate:CoA ligase 3791transcription factor IIB (TFIIB)792unknown protein793hypothetical protein794hypothetical protein795sugar transporter like protein796putative trypsin inhibitor797unknown protein798putative multispanning membraneprotein799receptor-like kinase, putative800putative inosine-5-monophosphatedehydrogenase801inosine-5′-monophosphatedehydrogenase, putative802amino acid permease 6(emb|CAA65051.1)803NADPH-ferrihemoproteinreductase (ATR2)804putative WRKY-type DNA bindingprotein805putative ankyrin806putative hexose transporter807aquaporin/MIP - like protein808Ser/Thr protein kinase isolog809pectate lyase like protein810putative 60S ribosomal protein L17811putative protein812unknown protein813phenylalanine ammonia-lyase814putative cytochrome P450monooxygenase815ARR1 protein, putative816putative bHLH transcription factor817aminomethyltransferase-likeprecursor protein818purple acid phosphatase precursor819AP2 domain containingprotein, putative820ubiquitin-conjugatingenzyme E2-21 kD 1(ubiquitin-protein ligase 4) (ubiquitin carrier protein 4)(sp|P42748)821translation initiation factor822putative VAMP-associatedprotein823spermidine synthase,putative824putative protein825unknown protein826AtKAP alpha827glyceraldehyde-3-phosphate dehydrogenase,putative828putative poly(A) bindingprotein829aipha-tubulin, putative830serine/threonine-specificprotein kinase ATPK64(pir||S20918)831putative aspartate-tRNAligase832ras-related small GTP-binding protein RAB1c833cycloartenol synthase834No function assigned byTIGR835cytochrome P450836GTPase AtRAB88373-phosphoserinephosphatase838transcription factor CRC839nuclear cap-bindingprotein; CBP20(gb|AAD29697.1)840chioroplast membraneprotein (ALBINO3)841biotin holocarboxylasesynthetase842expansin AtEx6843unknown protein844mercaptopyruvatesulfurtransferase, putative845putative thiosulfatesulfurtransferase846dihydrolipoamide 5-acetyltransferase847auxin transport protein REH1,putative848putative auxin transport protein849apyrase (Atapy1)850root cap 1 (RCP1)851hypothetical protein852putative protein853predicted protein of unknownfunction854hypothetical protein855hypothetical protein856hypothetical protein857putative aldehyde dehydrogenase858putative peroxidase859UDP-glucose 4-epimerase - likeprotein860indole-3-acetate beta-glucosyltransferase like protein861putative beta-1,3-glucanase862disease resistance protein-like863putative respiratory burst oxidaseprotein B864ubiquitin-conjugating enzymeUBC3865cytoplasmic aconitate hydratase866NADPH oxidoreductase, putative867PROTEIN TRANSPORTPROTEIN SEC61 GAMMASUBUNIT -like868putative protein869unknown protein87060S acidic ribosomal protein P2871No function assigned by TIGR8721,4-alpha-glucan branchingenzyme protein soform SBE2.2precursor873calcium binding protein (CaBP-22)874putative phosphoglucomutase875shaggy-like protein kinaseetha (EC 2.7.1.-)876pyruvate decarboxylase(gb|AAB16855.1)877hypothetical protein878putative protein kinase879putative protein kinase880putative leucineaminopeptidase881probable cytochrome P450882protein kinase 6-like protein883arginine methyltransferase(pam 1)884MYB96 transcriptionfactor-like protein885putative protein886metal ion transporter887No function assigned byTIGR888flax rust resistance protein,putative889fructose-2,6- homologbisphosphatase, putative890exonuclease RRP41891squamosa promoter bindingprotein-like 2(emb|CAB56576.1)892putative squamosa-promoter binding protein893O-acetylserine(thiol) lyase,putative894snoRNA895snoRNA896ferredoxin-NADP +reductase897H+-transporting ATPsynthase chain 9 - likeprotein898photosystem I subunit IIIprecursor, putative899photosystem I subunit VIprecursor900auxin-binding protein 1precursor901putative RAS superfamily GTP-binding protein902disease resistance protein-like903protein kinase like protein904glucuronosyl transferase-likeprotein905putative homeodomaintranscription factor906putative flavonol reductase907putative protein908salt-tolerance protein90940S ribosomal protein S30910putative bZIP transcription factor911putative protein912putative cinnamoyl CoA reductase913unknown protein914putative RNA-binding protein915phosphatidylinositol synthase(PIS1)916unknown protein917hydroxyproline-rich glycoprotein91850S ribosomal protein L15,chloroplast precursor919unknown protein920putative YME1 ATP-dependantprotease921unknown protein922putative ribosomal protein L28923unknown protein924putative protein925protein ch-42 precursor,chloroplast926protein serine/threonine kinase,putative927beta-VPE928putative vacuolar sorting receptor929putative translation initiation factorIF-2930predicted protein of unknownfunction931putative protein932hypothetical protein933hypothetical protein934phosphate transporter, putative935No function assigned by TIGR936beta subunit of proteinfamesyl transferase ERA1937putative glutamatedecarboxylase938putative indole-3-acetatebeta-glucosyltransferase939putative receptor-likeprotein kinase940UDP-galactose 4-epimerase-like protein941putative proliferating cellnuclear antigen, PCNA942ubiquitin conjugatingenzyme E2 (UBC13)943cyclophilin (CYP2)944cystatin(emb|CAA03929.1)945putative alcoholdehydrogenase946acidic ribosomal protein p1947glutathione transferase AtGST 10(emb|CAA10457.1)948putative tropinonereductase949ZIP4, a putative zinctransporter950unknown protein951putative protein952putative protein953putative C2H2-type zincfinger protein954putative RING zinc fingerprotein955putative microtubule-associated protein956unknown protein957putative protein958putative proteinphosphatase-2c959V-ATPase subunit G (vag2gene)960hypothetical protein961unknown protein962unknown protein963unknown protein964myrosinase-associated protein,putative965hypothetical protein966hypothetical protein967No function assigned by TIGR968unknown protein969hypothetical protein970LAXi / AUXi -like permease971putative UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase972chorismate mutase CM2973inner mitochondrial membraneprotein974DEF (CLA1) protein975decoy976citrate synthase977myosin97840S ribosomal protein S19979ripening-related protein - like980putative signal peptidase I981methionyl-tRNA synthetase(AtcpMetRS)982ribosomal protein precursor - like983505 ribosomal protein L21chloroplast precursor (CL21)984putative MYB family transcriptionfactor985cyclophilin - like protein986hypothetical protein987naringenin 3-dioxygenase likeprotein988WD-repeat protein -like protein989putative serine carboxypeptidase II990prenyltransferase, putative991putative ligand-gated ion channelprotein992clathrin adaptor medium chainprotein MU1B, putative993No function assigned by TIGR994putative Ta11-like non-LTR retroelement protein 995putative 3-isopropylmalatedehydrogenase9963-isopropylmalatedehydratase, small subunit997unknown protein998unknown protein999unknown protein1000hypothetical protein1001putative protein1002No function assigned byTIGR1003putative beta-glucosidase1004putative pectate lyase A111005putative beta-glucosidase1006HD-Zip protein1007putative ubiquitinconjugating enzyme1008homeobox-leucine zipperprotein-like1009cytochrome P450 likeprotein1010putative cysteine proteinaseinhibitor B (cystatin B) 1011ethylene response sensor(ERS)1012putative SWH1 protein1013putative glutathione S- transferase1014putative protein1015unknown protein1016putative proteinphosphatase 2C1017dnaJ protein homolog atj31018ferredoxin1019hypothetical protein1020putative sugar transport protein, ERD61021putative DnaJ protein1022putative AP2 domaintranscription factor1023putative protein1024putative cyclin-dependentkinase regulatory subunit1025putative tropinone reductase1026signal response protein (GAL)1027putative steroid sulfotransferase1028hypothetical protein1029nucleic acid binding protein - like1030putative protein1031blue copper binding protein1032famesylated protein (ATFP6)1033unknown protein1034putative PCF2-like DNA bindingprotein1035teosinte branched 1 - like protein1036putative protein1037unknown protein1038unknown protein10392-oxoglutarate dehydrogenase, E1component1040unknown protein1041unknown protein1042CCAAT-binding transcriptionfactor subunit A(CBF-A)1043hypothetical protein1044putative growth regulator protein1045putative presenilin1046putative expansin1047ribosomal - like protein1048unknown protein1049unknown protein1050putative protein1051putative protein1052unknown protein1053unknown protein1054unknown protein1055unknown protein1056unknown protein1057putative protein1058putative protein1059argininosuccinate lyase (AtArgH)1060disease resistance protein homolog1061aldehyde dehydrogenase likeprotein1062GBF2, G-box binding factor1063CDPK-related kinase1064endo-1,4-beta-glucanase1065putative serine protease1066serine/threonine-specifickinase lecRK1 precursor, lectinreceptor-like1067putative MAP kinase1068RNase L inhibitor-likeprotein1069No function assigned byTIGR1070AP2 domain transcriptionfactor1071polygalacturonaseisoenzyme 1 beta subunit,putative1072putative lipid transferprotein1073putative protein kinase1074putative protein1075ATP-dependent RNAhelicase like protein1076putative cyclic nucleotide-regulated ion channelprotein1077COP1 like protein1078putative peroxidase1079putative NAK-like ser/thrprotein kinase1080putative cytochrome C1081cytochromec1082putative serinecarboxypeptidase II1083acyl-(acyl carrier protein)thioesterase1084DNA-binding factor,putative1085MAP3K delta-1 proteinkinase1086AtMlo-h1-like protein1087No function assigned byTIGR1088putative expansin1089defender against cell deathprotein, putative1090glycolate oxidase - likeprotein1091putative ATP-dependent RNAhelicase1092putative protein1093putative HMG protein1094squalene monooxygenase 2(squalene epoxidase 2) (SE 2)(sp|O65403)1095eukaryotic peptide chain releasefactor subunit 1, putative1096auxin-induced protein - like1097putative lipoamide dehydrogenase1098putative protein1099unknown protein1100putative oligopeptide transporter1101putative translation elongationfactor ts1102putative CCAAT-bindingtranscription factor subunit1103putative ABC transporter1104putative superoxide-generatingNADPH oxidase flavocytochrome1105aspartate kinase-homoserinedehydrogenase - like protein1106putative bHLH transcription factor1107 putative geranylgeranyl transferasetype I beta subunit1108putative ARP2/3 protein complexsubunit p411109sulphite reductase1110putative auxin-regulated protein1111transcription factor scarecrow-like14, putative1112unknown protein1113monooxygenase 2 (MO2)1114putative amine oxidase1115zinc finger protein, putative1116DNA-binding protein, putative1117putative protein1118putative protein1119Avr9 elicitor response like protein1120putative protein1121hypothetical protein1122putative nucleotide-sugardehydratase1123UFD1 like protein1124putative trans-prenyltransferase1125outward rectifyingpotassium channel KCO1126unknown protein1127putativepectinacetylesterase1128putative protein1129No function assigned byTIGR1130unknown protein1131unknown protein1132unknown protein1133protein phosphatasehomolog (PPH1)1134unknown protein1135No function assigned byTIGR1136unknown protein1137unknown protein1138unknown protein1139putative protein1140unknown protein1141putative ubiquinol--cytochrome-c reductase1142unknown protein1143contains similarity to high- glucose-regulated protein 8GB:AAF08813 GI:6449083 from [Homo sapiens]1144unknown protein1145putative cis-Golgi SNAREprotein1146unknown protein1147glutamate-1-semialdehydeaminotransferase1148No function assigned byTIGR1149hypothetical protein1150unknown protein1151unknown protein1152unknown protein1153scarecrow-like 31154putative proline-rich protein1155cytoebrome c oxidoreductase likeprotein1156putativecarboxymethylenebutenolidase1157unknown protein1158unknown protein1159unknown protein1160unknown protein1161unknown protein1162unknown protein1163auxin-induced protein (IAA20)1164sos ribosomal protein L41165putative DNA topoisomerase IIIbeta1166No function assigned by TIGR1167isp4 like protein1168putative protein kinase1169hypothetical protein1170putative pyrophosphate--fructose-6-phosphate 1-phosphotransferase1171putative protein1172putative protein1173putative protein1174unknown protein1175unknown protein1176putative protein1177putative protein1178unknown protein1179unknown protein1180putative protein1181brassinosteroid insensitive 1 gene(BRI1)1182putative receptor protein kinase1183vacuolar-type H+-translocatinginorganic pyrophosphatase1184protein kinase - like protein1185glycyl tRNA synthetase, putative1186subtilisin proteinase - like1187hypothetical protein1188cytochrome P450-like protein1189cytochrome p450 like protein1190putative protein kinase1191pectinesterase - like protein1192putative receptor-like proteinkinase1193peroxidase ATP17a -likeprotein1194No function assigned byTIGR1195cellulose synthase catalytic subunit - like protein1196RAS-related protein, RAB71197putative aspartateaminotransferase1198cyclophilin1199putative SF2/ASF splicingmodulator, Srp301200putative cytoebrome b51201glutamyl-tRNA reductase,putative1202putative MADS-box protein1203ammonium transportprotein (AMT1)1204No function assigned byTJGR1205putative beta-ketoacyl-CoAsynthase1206thaumatin-like protein1207putative methionineaminopeptidase1208putative proteinphosphatase 2C1209kinase-like protein1210receptor-associated kinaseisolog1211mitochondrial ribosomalprotein S141212oleosin, 18.5 K1213chalcone isomerase1214putative cyclin-dependentkinase regulatory subunit1215putative thaumatin-likeprotein1216putative two-componentresponse regulator protein1217TATA binding protein-associated factor, putative1218predicted protein ofunknown function1219putative AP2 domain transcriptionfactor1220brassinosteroid receptor kinase,putative1221TINY-like protein1222glucose-6-phosphate isomerase1223putative protein1224putative NAM (no apicalmeristem)-like protein1225unknown protein1226putative nucleotide-binding protein1227bZIP transcription factor (POSF21)1228ubiquitin activating enzyme - likeprotein1229telomere repeat-binding protein1230unknown protein1231mevalonate kinase1232putative protein1233hypothetical protein1234disease resistance RPP5 likeprotein1235putative protein1236putative pectinesterase1237Ttg1 protein (emb|CAB45372.1)1238FUSCA PROTEIN FUS61239NHE1 Na+/H+ exchanger1240No function assigned by TIGR1241Phospholipase like protein1242unknown protein1243unknown protein1244unknown protein1245AUX1-like amino acid permease1246unknown protein1247putative C2H2-type zinc fingerprotein1248putative protein1249putative protein1250putative glucosyltransferase1251putative lipase1252putative protein1253putative thioredoxin1254AIG2-like protein1255short-chain alcohol dehydrogenaselike protein1256hypothetical protein1257putative protein1258putative protein1259glutathione peroxidase -like protein1260putative protein1261putative disease resistanceresponse protein1262putative protein1263senescence-associatedprotein (SAG29)1264glycolate oxidase, putative1265extensin - like protein1266putative protein1267unknown protein1268putative disease resistanceprotein1269putative receptor-likeprotein kinase1270putative receptor-likeprotein kinase1271basic chitinase1272putative pectinmethylesterase1273peroxidase ATP N1274class 2 non-symbiotichemoglobin1275nitrate transporter1276Ca2+/H+-exchangingprotein-like1277putative protein1278hydroxynitrile lyase likeprotein1279putative AP2 domaintranscription factor1280pectin methylesterase,putative1281putative protein1282beta-glucosidase-likeprotein1283CCAAT box binding factor/transcription factor Hap2a1284putative fibrillin1285xyloglucan endo-transglycosylase1286putative 10kd chaperonin1287No function assigned by TIGR1288serine/threonine protein kinaseATPK101289putative lipase1290choline kinase GmCK2p-likeprotein1291putative sugar transport protein,ERD61292MYB27 protein - like1293DNA-binding protein, putative1294similar to cold acclimation proteinWCOR4 13 [Triticum aestivum]1295unknown protein1296aquaporin (plasma membraneintrinsic protein 2B)1297No function assigned by TIGR1298P-Protein - like protein1299No function assigned by TIGR1300putative cytochrome P450monooxygenase1301putative cytochrome P450monooxygenase1302putative thioredoxin1303stromal ascorbate peroxidase1304ethylene responsive elementbinding factor-like protein(AtERF6)1305auxin transport protein EIR1(gb|AAC39513.1)1306putative CONSTANS-like B-boxzinc finger protein1307putative protein kinase1308mitochondrial Lon proteasehomolog 1 precursor (sp|O64948)1309putative protein1310heme activated protein, putative1311putative cytochrome P4501312No function assigned by TIGR1313putative lipase1314putative protein1315putative sugar transporter protein1316putative sucrose transport protein,SUC21317putative protein1318putative protein1319putative endochitinase1320putative acetone-cyanohydrin lyase1321putative protein1322calmodulin-like protein1323hypothetical protein1324cysteine proteinase likeprotein1325heat shock protein 17.6-II1326heat shock protein 181327Arabidopsis mitochondrion-localized small heat shockprotein (AtHSP23.6-mito)1328unknown protein1329putative WRKY-type DNAbinding protein1330No function assigned byTIGR1331hypothetical protein1332putative integral membraneprotein nodulin1333putative protein1334unknown protein13353-isopropylmalatedehydratase, small subunit1336unknown protein1337putative homeodomaintranscription factor1338unknown protein1339putative protein1340peroxidase ATP19a1341putative Na+/H+-exchanging protein1342putative auxin-regulatedprotein1343unknown protein1344unknown protein1345putative trehalose-6-phosphate synthase1346putative lectin1347Mlo protein-like1348unknown protein1349ethylene response factor,putative1350unknown protein1351unknown protein1352bZIP transcription factor - likeprotein1353Medicago nodulin N21-like protein1354putative endo-1,4-beta glucanase13551-aminocyclopropane-1-carboxylate oxidase1356putative anion exchange protein1357SRG1-like protein1358putative protein1359putative phi-1-like phosphate-induced protein1360putative protein1361putative embryo-abundant protein1362putative hydrolase1363unknown protein1364unknown protein1365hexose transporter - like protein1366unknown protein1367unknown protein1368peptide transport - like protein1369unknown protein1370putative peptide transporter1371disease resistance protein, putative1372cysteine protease component ofprotease-inhibitor complex1373putative cytochrome P4501374putative protein1375hypothetical protein1376unknown protein1377putativephosphoribosylaminoimidazolecarboxamide formyltransferase1378putative protein1379HSP like protein1380unknown protein1381unknown protein1382putative cytochrome P4501383similar to pectinesterase1384putative glucosyltransferase1385thaumatin-like protein1386drought-inducible cysteineproteinase RD19A precursor1387vegetative storage protein Vsp21388unknown protein1389unknown protein1390anthranilate N-benzoyltransferase - likeprotein1391delta-1-pyrroline 5-carboxylase synthetase(P5C1)1392glutathione S-conjugatetransporting ATPase(AtMRP1)1393hypothetical protein1394hypothetical protein1395unknown protein1396putative protein1397putative protein1398No function assigned byTIGR1399unknown protein1400putative protein kinase1401unknown protein1402hypothetical protein1403unknown protein1404putative calcium-bindingEF-hand protein1405cinnamyl-alcoholdehydrogenase ELI3-11406putative protein1407unknown protein1408senescence-associatedprotein sen11409hypothetical protein1410putative cytochrome P4501411proline oxidase,mitochondrial precursor(osmotic stress-inducedproline dehydrogenase)1412putative response regulator31413hypothetical protein1414glutamine-dependentasparagine synthetase1415lysine-ketoglutaratereductase/saccharopine1416En/Spm-like transpo sonprotein1417G-box binding bZIP transcriptionfactor1418putative protein1419putative protein1420putative protein1421ATFP4-like1422unknown protein1423unknown protein1424putative protein1425invertase inhibitor homolog(emb|CAA73335.1)1426unknown protein1427unknown protein1428putative cytochrome b51429putative protein1430putative protein1431putative protein1432No function assigned by TIGR1433putative copper/zinc superoxidedismutase1434protein phosphatase ABI11435glutamate dehydrogenase 21436No function assigned by TJGR1437low-temperature-induced protein78 (sp|Q06738)1438putative myo-inositol 1-phosphatesynthase1439phosphate transporter(gb|AAB17265.1)14404-hydroxyphenylpyruvatedioxygenase (HPD)1441histone H11442hypothetical protein1443No function assigned by TIGR1444neoxanthin cleavage enzyme-likeprotein1445dehydration-induced protein RD221446zinc finger protein ZAT71447unknown protein1448unknown protein1449unknown protein1450unknown protein1451putative protein1452putative protein1453RNA helicase, putative1454putative glycine-richprotein1455hypothetical protein1456putative protein1457peroxidase1458peroxidase ATP3a(emb|CAA67340.1)1459metallothionein-like protein1460endomembrane-associatedprotein1461ferritin 1 precursor1462dehydrin RAB18-likeprotein (sp|P30185)1463HSR201 like protein1464light regulated protein,putative1465Dr4(protease inhibitor)1466mitogen activated proteinkinase kinase (nMAPKK)1467glutathione S-transferase1468transcriptional activatorCBF1/CRT/CRE bindingfactor 11469homeobox-leucine zipperprotein ATHB-121470amino acid permease I1471MAP kinase (ATMPK7)1472potassium channel proteinAKT31473cytochrome P450monooxygenase(CYP91A2)1474putative transport protein1475putative protein1476hypothetical protein1477putative protein1478hypothetical protein1479receptor protein kinase-likeprotein1480serine/threonine proteinkinase - like protein1481putative auxin-regulatedprotein1482amino acid transport proteinAAP21483unknown protein1484cold and ABA inducible proteinkin11485gamma-VPE (vacuolar processingenzyme)1486putative protein 1 photosystem IIoxygen-evolving complex1487myrosinase-associated protein,putative1488transcription factor ATMYB41489H-protein promoter binding factor-2a1490ammonium transporter, puitative1491putative zeta-carotene desaturaseprecursor1492high-affinity nitrate transporterNRT21493light induced protein like1494putative AT-hook DNA-bindingprotein1495putative glycogenin1496putative light repressible receptorprotein kinase1497serine/threonine kinase - likeprotein1498putative peroxidase1499cytochrome P450 monooxygenase(CYP83A1)1500MYB-related transcription factor(CCA1)1501Terminal flower1 (TFL1)1502sulfate transporter ATST11503RING-H2 finger protein RHA3b1504lipoxygenase, putative1505serine O-acetyltransferase (BC2.3.1.30) Sat-52 (pir||S71207)1506ferulate-5-hydroxylase (FAH1)1507En/Spin-like transposon protein,putative1508calmodulin-binding - like protein1509hypothetical protein1510somatic embryogenesis receptor-like kinase - like protein1511putative giberellin beta-hydroxylase1512putative pectinesterase 1513putative protein1514unknown protein1515ribosomal protein1516low-temperature-induced 65 kD protein (sp|Q04980)1517putative glucosyltransferase1518peroxidase(emb|CAA67551.1)1519ankyrin-like protein1520ribosomal protein S11 - like1521hypothetical protein1522glycoprotein(EP1), putative1523calnexin - like protein1524SRG1-like protein1525ethylene response factor 1(ERF1)1526transcriptional activatorCBF1-like protein1527xyloglucan endo-1,4-beta-D-glucanase (XTR-6)1528putative cinnamyl alcoholdehydrogenase1529gibberellin 3 beta-hydroxylase, putative1530auxin response transcriptionfactor 3 (ETTIN/ARF3)1531No function assigned byTIGR1532putative protein1533similar to avrRpt2-inducedprotein 11534unknown protein1535hypothetical protein1536putative protein kinase 1537respiratory burst oxidase -like protein1538glucose-6-phosphate/phosphate-translocator precursor,putative1539class 1 non-symbiotichemoglobin (AHB1)1540endochitinase isolog1541putative cytochrome P450154260S acidic ribosomal protein P01543putative protein1544auxin-induced protein, putative1545unknown protein1546hypothetical protein1547protein phosphatase 2C ABI2(PP2C) (sp|O04719)1548peroxidase, prxr21549putative peroxidase ATP12a1550putative beta-amylase1551putative acetone-cyanohydrin lyase1552fatty acid elongase 3-ketoacyl-CoAsynthase 11553putative citrate synthase1554pEARLI 1-like protein1555putative MYB family transcriptionfactor1556putative transcription factorMYB281557RINA helicase-like protein1558snoRNA1559putative protein kinase1560growth regulator like protein1561putative potassium transporter1562putative protein156360S ribosomal protein L141564unknown protein1565putative RING-H2 zinc fingerprotein1566putative pollen surface protein1567unknown protein1568unknown protein1569unknown protein1570putative Ca2+-ATPase15711-aminocyclopropane-1-carboxylate synthase -like protein1572putative beta-glucosidase1573transcription factor ZAP11574oligopeptide transporter, putative1575putative protein1576putative glucosyltransferase1577putative serine/threonine kinase1578squalene epoxidase - like protein1579similar to 14KD proline-richprotein DC2.15 precursor(sp|P14009); similar toESTs emb|Z17709 andemb|Z476851580unknown protein1581unknown protein1582hypothetical protein158360S ribosomal protein L381584flavin-containingmonooxygenase, putative1585remorin1586unknown protein1587putative protein1588lipoxygenase1589cold-regulated proteinCOR6.6 (KIN2)1590Myb transcription factorhomolog (ATR1)1591putative protein1592unknown protein1593unknown protein1594Ca2+-transporting ATPase -like protein1595protein phosphatase 2C(AtP2C-HA)1596peroxidase ATP24a1597branched-chain alpha keto-acid dehydrogenase,putative1598putative beta-ketoacyl-CoAsynthase1599putative protein1600putative beta-galactosidase1601putative protein160260S ribosomal protein L271603putative annexin1604NAC domain protein,putative1605unknown protein1606late embryo genesisabundant protein LEA like1607unknown protein1608putative protein1609dehydrin Xero21610putative zinc finger protein1611unknown protein1612DnaJ-like protein1613putative inositol polyphosphate-5-phosphatase1614putative cytochrome P4501615putative protein1616unknown protein1617putative protein1618hypothetical protein1619putative protein1620sucrose-UDP glucosyltransferase1621glucose-6-phosphate 1-dehydrogenase1622unknown protein1623mitochondrial chaperonin (HSP60)1624sucrose transport protein SUC11625putative protein disulfide isomerase1626putative pollen-specific protein1627integral membrane protein,putative1628rubredoxin, putative1629putative protein1630disease resistance protein RPS4,putative1631putative peptide/amino acidtransporter1632peroxidase, putative1633ethylene receptor, putative (ETR2)1634protein phosphatase 2C (PP2C)1635putative glutathione S-transferase1636homeodomain transcription factor(ATHB-7)1637putative nitrate transporter1638putative ribosomal protein L9,cytosolic1639putative DNA-binding protein1640beta-1,3-glucanase-like protein1641putative zinc transporter1642transcription factor TINY1643putative aspartate kinase-homoserine dehydrogenase1644ethylene reponse factor-like AP2domain transcription factor1645peptide transporter - like protein1646trehalose-6-phosphate synthase likeprotein1647putative ribonuclease1648hypothetical protein1649putative DNA-bindingprotein1650nodulin-like protein1651trehalose-6-phosphatephosphatase - like protein1652succinate dehydrogenaseflavoprotein alpha subunit(emb|CAA05025.1)1653unknown protein1654stress related protein,putative1655putative chioroplastinitiation factor 31656putative protein1657hypothetical protein1658putative CCCH-type zincfinger protein1659similar to harpin-inducedprotein hin1 from tobacco1660unknown protein1661unknown protein1662hypothetical protein1663No function assigned byTIGR1664putative protein1665putative glutathione S-transferase TSI-11666putative protein1667putative PTR2 familypeptide transporter1668receptor kinase-like protein1669putative sugar transportprotein, ERD61670putative protein1671nodulin-like protein1672unknown protein1673putative receptor-likeprotein kinase1674glutathione-conjugatetransporter AtMRP41675ascorbate oxidase-likeprotein1676pathogenesis-related protein 1precursor, 19.3 K1677R2R3-MYB transcription factor1678hypothetical protein1679putative chitinase1680Mb protein, putative1681putative WRKY-type DNA bindingprotein1682putative acyl-CoA synthetase1683putative pathogenesis-relatedprotein1684putative chitinase1685germin precursor oxalate oxidase1686endoxyboglucan transferase,putative1687putative protein1688putative cytoclirome P4501689similar to Mb proteins from H.vulgare1690putative tropinone reductase1691extensin-like protein1692putative sarcosine oxidase1693putative protein1694hypothetical protein1695late embryogenesis-abundantprotein, putative1696beta-carotene hydroxylase1697putative calcium binding protein1698unknown protein1699unknown protein1700predicted glycosyl transferase1701hypothetical protein1702hypothetical protein1703hypothetical protein1704putative protein1705unknown protein1706putative protein1707putative protein1708serine/threonine kinase - likeprotein1709No function assigned by TIGR1710putative pectinesterase1711peroxidase like protein1712No function assigned by TIGR1713phenylalanine ammonialyase (PAL1)1714peroxidase(emb|CAA68212.1)1715putative AMP deaminase1716putative MYB familytranscription factor1717DNA-directed RINApolymerase II, third largest subunit1718nucleotide pyrophosphatase-like protein1719putative peroxidase1720calcium sensor homolog(gb|AAC26110.1)1721putative GDSL-motiflipase/hydrolase1722putative nonspecific lipid-transfer protein1723acyl-carrier protein (ACP),putative1724putative glycinedehydrogenase1725AIG11726ACC synthase (AtACS-6)1727cyclin delta-31728putative RING zinc fingerprotein1729aldose 1-epimerase - likeprotein1730putative phospholipase1731phosphoenolpyruvatecarboxylase1732putative galactinol synthase1733unknown protein1734putative protein17351-aminocyclopropane-1-carboxylate oxidase1736thioredoxin (clone GIF1)(pir||S58118)1737trehalose-6-phosphatephosphatase1738beta-1,3-glucanase 2 (BG2)(PR-2)1739putative S-adenosyl-L-methionine:trans-caffeoyl-Coenzyme A 3-O-methyltransferase1740disease resistance protein EDS11741putative protein kinase1742Gluthatione reductase, chioroplastprecursor1743putative heat shock protein1744aspartate kinase1745putative major intrinsic (channel)protein1746matrix metalloproteinase, putative1747putative GDSL-motiflipase/hydrolase1748putative protein1749DAG-like protein1750serine/threonine kinase -likeprotein1751formamidase - like protein1752CER2175326S proteasome subunit 41754pectinesterase like protein1755putative disease resistance protein1756putative RNA methyltransferase1757unknown protein1758HOMEOBOX PROTEiNKNOTTED-1 LIKE 4 (KNAT4)1759glycine-rich RINA-binding proteinAtGRP2 - like1760putative acetylomithinetransaminase1761putative Sec24-like COPII protein1762putative berberine bridge enzyme1763putative GH3-like protein1764putative ABC transporter1765putative reticuline oxidase-likeprotein1766pectate lyase - like protein1767protein disulfide-isomerase-likeprotein1768putative protein1769putative membrane transporter1770unknown protein1771unknown protein1772putative RING-H2 zinc fingerprotein1773unknown protein1774unknown protein1775unknown protein1776MADS-box protein(AGL20)1777amidophosphoribosyltransferase 2 precursor1778putative dihydrodipicolinatesynthase1779hypothetical protein1780ABA-responsive protein -like1781putative protein1782hypothetical protein1783DNA-binding protein-like1784No function assigned byTIGR1785transcription factor,putative1786nitrate reductase, putative1787putative protein1788putative protein1789putative protein1790putative protein1791unknown protein1792unknown protein1793tryptophan synthase beta-subunit (TSB2)1794hypothetical protein1795putative protein1796putative DNA-bindingprotein1797putative 40S ribosomalprotein S101798putative protein1799putative cytochrome P4501800putative protein1801putative protein1802putative glucosyltransferase1803No function assigned byTIGR1804putative protein1805putative protein1806unknown protein1807glycine-rich RNA binding protein71808dehydrin, putative1809putative endoxyloglucanglycosyltransferase1810glutamate decarboxylase 1 (GAD1) (sp|Q42521)1811delta 9 desaturase1812UDP-glucose glucosyltransferase1813CARBONIC ANI-JYDRASE 21814response reactor 2 (ATRR2)1815S-adenosyl-methionine-sterol-C-methyltransferase, putative1816putative DNA-binding protein(RAV2-like)1817gamma glutamyl hydrolase,putative1818protein phosphatase - like1819unknown protein1820unknown protein1821unknown protein1822copper transport protein - likeprotein1823hypothetical protein1824unknown protein1825putative peptide methioninesulfoxide reductase1826putative obtusifoliol 14-alphademethylase1827glutamate dehydrogenase (EC1.4.1.-) 1 (pir||S71217)1828unknown protein1829xyloglucan endo-1,4-beta-D-glucanase precursor1830unknown protein1831SNF1 related protein kinase(ATSRPK1)1832putative protein1833putative chloroplast nucleoid DNAbinding protein1834hypothetical protein1835putative protein1836putative thiamin biosynthesisprotein1837unknown protein1838unknown protein1839putative RNA helicase1840putative SF21 protein{Helianthus annuus}1841unknown protein1842NBS/LRR diseaseresistance protein, putative1843hypothetical protein1844unknown protein1845No function assigned byTIGR1846glycine-rich protein(AtGRP2)1847No function assigned byTIGR1848putative protein1849putative glucosyltransferase1850hypothetical protein1851hypothetical protein1852putative protein1853putative disease resistanceprotein1854thaumatin, putative1855putative proline-rich protein1856sterol-C-methyltransferase1857superoxidase dismutase1858TINY-like protein1859calcium-dependent proteinkinase, putative1860hypothetical protein1861putative protein kinase1862DNA-directed RNApolymerase (mitochondrial)1863putaive DNA-bindingprotein1864late embryo genesisabundant M17 protein1865putative protein1866delta-1-pyrroline-5-carboxylate synthetase1867putative 60s ribosomalprotein L101868cytochrome P450CYP86A11869putative tyrosine aminotransferase1870thionin1871No function assigned by TIGR1872APETALA2 protein1873MADS-box protein (AGL3)1874putative monooxygenase1875ZFP3 zinc finger protein1876cell division protein FtsZchloroplast homolog precursor(sp|Q42545)1877calreticulin, putative1878phosphoserine aminotransferase187912-oxophytodienoate-10,11-reductase1880putative bHLH transcription factor1881pectin methylesterase (PMEU1),putative1882DNA-binding protein1883carnitine racemase like protein1884putative protein1885endoxyloglucan transferase(dbj|BAA81669.1)1886RMA1 RING zinc finger protein1887ammonium transporter1888apyrase (gb51 AAF00612.1)1889potassium uptake transporter - likeprotein1890putative ABC transporter1891potassium transporter-like protein1892integral membrane protein,putative1893putative protein1894pyruvate decarboxylase-1 (Pdc1)1895putative malate oxidoreductase1896putative histone H2B1897snoRNA1898symbiosis-related like protein1899unknown protein1900unknown protein1901hypothetical protein1902putative protein1903copper-binding protein-like1904putative protein1905unknown protein1906putative glyoxalase II1907No function assigned byTIGR1908hypothetical protein1909flavanone 3-hydroxylase(FH3)1910putative laccase1911putative protein kinase1912myb-related protein, 33.3K(pir||S71284)1913unknown protein1914endo-xyloglucan transferase -like protein1915TMV resistance protein N -like1916putative xyloglucanendotransglycosylase1917unknown protein1918proline transporter 21919resistance protein, putative1920actin, putative1921putative related to microbialdivalent cation toleranceproteins1922unknown protein1923putative glycosyltransferase1924unknown protein1925putative proteinphosphatase 2C1926unknown protein1927serpin, putative1928cinnamyl-alcoholdehydrogenase CAD11929putative protein importreceptor1930unknown protein1931unknown protein1932putative protein1933putative CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase1934unknown protein1935putative LRR receptor-likeprotein kinase1936serine/threonine protein kinase,putative1937potassium transporter - like protein1938lactate dehydrogenase (LDH1)1939hypothetical protein1940unknown protein1941putative thaumatin1942putative reticuline oxidase-likeprotein1943uracil phosphoribosyltransferase,putative1944transcription factor, putative1945unknown protein1946unknown protein1947GATA transcription factor 41948unknown protein1949unknown protein1950senescence-associated protein -like1951putative pollen allergen1952unknown protein1953putative protein1954glycine-rich protein1955putative protein19563-methyladenine DNA glycosylase,putative1957endoplasmic reticulum-typecalcium-transporting ATPase 41958putative pectinesterase1959cytochrome P450-like protein1960RNA-binding protein (cp33)1961CONSTANS-like 11962putative small heat shock protein1963hypothetical protein1964unknown protein1965cytochrome P450 - like protein1966cysteine proteinase inhibitor likeprotein1967nicotianamine synthase(dbj|BAA74589.1)1968copper amine oxidase like protein(fragment2)1969putative SCARECROW generegulator1970unknown protein1971unknown protein1972putative alanine acetyl transferase1973unknown protein1974unknown protein1975unknown protein1976putative extensin1977putative protein kinase1978putative protein kinase1979NADPH-dependentcodeinone reductase,putative1980peroxidase1981putative cytochrome P4501982No function assigned byTIGR1983putative zinc-finger protein(B-box zinc finger domain)1984putative tyrosineaminotransferase1985hypothetical protein1986DNA binding protein1987putative fatty acid elongase1988bZIP transcription factor -like protein1989xyloglucanfucosyltransferase, putative1990unknown protein1991unknown protein1992putative protein1993myb factor, putative1994Myb-family transcriptionfactor, putative1995putative fructosebisphosphate aldolase1996myrosinase-associatedprotein, putative1997cytochrome P450 likeprotein1998similar to SOR1 from thefungus Cercosporanicotianae1999similar to embryo-abundantprotein GB:L47672 GI:1350530from [Picea glauca]2000alcohol dehydrogenase2001auxin response factor 12002pathogenesis-related protein 1precursor, 18.9K2003hypothetical protein2004unknown protein2005zinc finger protein Zat122006unknown protein2007unknown protein2008cyclin, putative20092-dehydro-3 -deoxyphosphoheptonate aldolase2010glutathione synthetase gsh22011heat shock protein 172012putative Na+-dependent inorganicphosphate cotransporter2013No function assigned by TIGR2014unknown protein2015putative protein2016similar to RING-H2 finger proteinRHC1a GB:AAC69854GI:3790583 from [Arabidopsisthaliana]2017calcium-binding protein - like2018putative protein2019putative aldehyde dehydrogenase2020auxin-responsive GH3 - likeprotein2021putative protein2022Phosphoglycerate dehydrogenase -like protein2023unknown protein2024unknown protein2025PSI type III chlorophyll a/b-binding protein, putative2026putative protein2027putative protein2028glutaredoxin, putative2029hypothetical protein2030No function assigned by TIGR2031putative protein2032jasmonate inducible protein,putative2033putative polygalacuronaseisoenzyme 1 beta subunit2034putative small heat shock protein2035unknown protein2036putative disease resistanceprotein2037putative protein2038ethylene-responsiveelement binding factor,putative2039putative protein2040Pollen-specific proteinprecursor like2041putative protein2042unknown protein2043EF-Hand containing protein-like2044unknown protein2045puative calcium-transporting ATPase2046antifungal protein-like (PDF1.2)2047pathogenesis-related PR-1-like protein2048similar to Mlo proteinsfrom H. vulgare2049putative steroidsulfotransferase2050trehalase - like protein2051thioredoxin f12052unknown protein2053alanine-glyoxylateaminotransferase2054integral membrane protein,putative2055hypothetical protein2056unknown protein2057hypothetical protein2058unknown protein2059unknown protein2060unknown protein2061drought-induced-19-like 12062unknown protein2063putative protein2064putative protein2065AIG2-like protein2066Lhca2 protein2067phytocyanin2068putative chlorophyll A-B bindingprotein2069Lhcb3 chlorophyll a/b bindingprotein (gb|AAD28773.1)2070luminal binding protein(dbj|BAA13948.1)2071hydroxypyruvate reductase (HPR)2072epoxide hydrolase (ATsEH)2073putative protein (fragment)2074unknown protein2075hypothetical protein2076putative glucosyl transferase2077putative glucosyl transferase2078putative 3-methylcrotonyl-CoAcarboxylase2079putative peroxidase2080acyl-CoA oxidase(gb|AAC13497.1)2081alternative oxidase 1a precursor2082putative transcription factor(MYB4)2083serine acetyltransferase2084ATP-sulfurylase2085calreticulin (crt1)2086putative prohibitin 22087putative monodehydroascorbatereductase2088branched-chain alpha-keto aciddecarboxylase E1 beta subunit2089cytokinin oxidase - like protein2090putative receptor-like proteinkinase2091unknown protein2092hypothetical protein2093No function assigned by TIGR2094putative APG protein2095glutathione S-transferase, putative2096phytochrome-associated protein 1(PAP1)2097amidophosphoribosyltransferase2098nonphototropic hypocotyl 120993-keto-acyl-CoA thiolase 2(gb|AAC17877.1)2100pEARLI 12101glutathione reductase, cytosolic2102putative protein2103putative protein2104putative aldehyde oxidase2105probable photosystem Ichain XI precursor2106photo system II polypeptide,putative2107photosystem II reactioncenter 6.1 KD protein210833 kDa polypeptide ofoxygen-evolving complex(OEC) in photosystem II(emb|CAA75629.1)210960S ribosomal proteinL11B2110extA (emb|CAA47807.1)2111zinc finger protein OBP4 -like2112sterol delta7 reductase2113putative RAS-relatedprotein, RAB11C2114glucosyltransferase likeprotein2115zinc finger protein (PMZ),putative21166,7-dimethyl-8-ribityllumazine synthaseprecursor2117putative protein2118osmotin precursor2119No function assigned byTIGR2120ferredoxin precusor isolog2121GH3 like protein2122non-specific lipid transferprotein2123homeodomain transcriptionfactor (HAT9)2124putative cytochrome P450monooxygenase2125putative protein kinase2126putative protein2127glyceraldehyde-3-phosphate dehydrogenase2128putative protein disulfide-isomerase2129unknown protein2130beta-1,3-glucanase class Iprecursor2131homeobox-leucine zipper proteinHAT5 (HD-ZIP protein 5) (HD-ZIP protein ATHB-1)2132putative cyclic nucleotide-regulated ion channel protein2133P II nitrogen sensing protein GLB I2134H-protein promoter binding factor-1 (gb|AAC24592.1)2135GAST1-like protein2136cytochrome P450 GA32137putative protein2138Myb-related transcription factor-like protein2139putative phloem-specific lectin2140protein kinase - like protein2141unknown protein2142SCARECROW transcriptionalregulator-like2143unknown protein2144unknown protein2145putative protein2146calnexin homolog2147PP1/PP2A phosphatasespleiotropic regulator PRL22148xyloglucan endotransglycosylase,putative2149putative calmodulin2150spermine synthase (ACL5)2151snoRNA2152photosystem I subunit V precursor,putative2153putative potassium transporter2154Homeodomain - like protein2155putative protein2156unknown protein2157CALMODULIN-RELATEDPROTEIN 2, TOUCH-INDUCED(TCH2)2158putative protein phosphatase 2C2159monosaccharide transportprotein, STP42160hypothetical protein2161unknown protein2162hypothetical protein2163putative protein kinase2164putative serine/threonineprotein kinase2165jasmonate inducibleprotein, putative2166similar to several smallproteins (˜100 aa) that areinduced by heat, auxin,ethylene and woundingsuch as Phaseolus aureusindole-3-acetic acidinduced protein ARG(SW:32292)2167unknown protein2168MYB-like protein2169putative protein kinase2170unknown protein2171CLC-d chloride channelprotein2172cytochrome P450-likeprotein2173putative glutathione 5-transferase2174putative mandelonitrilelyase2175hypothetical protein2176putative trypsin inhibitor2177male sterility 2-like protein(emb|CAA68191.1)2178unknown protein2179unknown protein2180putative protein2181putative peroxidase2182putative thromboxane-Asynthase2183putative cytochrome P4502184peroxidase ATP21a2185unknown protein2186putative glutathione S-transferase2187defender against cell death protein2188AP2 domain containing protein,putative2189actin depolymerizing factor - likeprotein2190putative calcium-dependent proteinkinase (U90439)2191phosphoribosylanthranilatetransferase, putative2192oligopeptide transporter, putative2193calmodulin-like protein2194putative protease inhibitor2195MAP kinase2196DNA binding protein MybSt1,putative2197putative protein2198putative protein2199unknown protein2200unknown protein2201unknown protein2202putative protein2203unknown protein2204unknown protein2205hypothetical protein2206uncharacterized protein2207putative protein2208hypothetical protein2209peroxidase (emb|CAA66967.1)2210putative flavonol 3-O-glucosyltransferase2211putative flavonol 3-O-glucosyltransferase2212putative protein2213glycerol-3-phosphateacyltransferase2214putative beta-1,3-glucanase2215putative ethylene response elementbinding protein (EREBP)2216putative CONSTANS-like B-boxzinc finger protein2217putative protein2218unknown protein2219putative trehalose-6-phosphatephosphatase (AtTPPA)2220putative protein2221putative protein2222unknown protein2223unknown protein2224unknown protein2225hypothetical protein2226putative metal-bindingprotein2227putativephosphoribosyiglycinamidesynthetase2228unknown protein2229putative protein2230unknown protein2231unknown protein2232putative beta-galactosidase2233putative protein kinase2234putative protein2235putative proteinphosphatase 2C2236putative growth regulatorprotein2237putative ABC transporter2238chloride channel(emb|CAA70310.1)2239adrenodoxin - like protein2240NAM (no apical meristem)-like protein2241putative transcription factorMYB412242Myb DNA binding protein -like2243AtMYB842244photosystem II type Ichlorophyll a/b bindingprotein2245putative aspartic proteinase2246jasmonate inducibleprotein, putative2247putative protein2248No function assigned byTIGR2249putative phosphatidylserinesynthase2250putative nicotianaminesynthase2251lysine and histidine specifictransporter, putative2252putative protein2253putative protein2254putative sugar transporter protein225512S cruciferin seed storage protein2256putative auxin-induced protein,IAA17/AXR3-12257putative cyclin D2258farnesyl diphosphate synthaseprecursor (gb|AAB49290.1)2259putative potassium transportprotein (TRH1)2260putative NPK1 -related MAP kinase2261putative protein2262putative ABC transporter2263putative DNA-directed RNApolymerase subunit2264putative small nuclearribonucleoprotein E2265unknown protein2266reticuline oxidase - like protein2267putative 1-aminocyclopropane-1-carboxylate oxidase2268similar to Mlo proteins from H.vulgare2269long-chain-fatty-acid--CoA ligase-like protein2270putative protein2271chromatin remodelling complexATPase chain ISWI -like protein2272hypothetical protein2273latex-abundant protein, putative2274N-acetybornithine deacetylase-likeprotein, fragment2275putative DNA-binding protein2276putative anthranilate N-hydroxycinnamoyl/benzoyltransferase2277putative DNA binding protein2278cytochrome P450 - like protein2279putative DNA-binding protein2280putative peptide transporter2281putative reticuline oxidase-likeprotein2282thioredoxin, putative2283nodulin-like protein2284UDP-galactose transporter -like protein2285putative fibrillin2286unknown protein2287unknown protein2288unknown protein2289hypothetical protein2290glyceraldehyde 3-phosphatedehydrogenase A subunit(GapA)2291predicted protein ofunknown function2292putative protein2293putative protein2294myb-like protein2295hypothetical protein2296putative US small nuclearribonucleoprotein, an RNAhelicase2297unknown protein2298cinnamyl alcoholdehydrogenase - likeprotein2299hypothetical protein similarto extensin-like protein2300unknown protein2301putative chlorophyll a/bbinding protein2302probable plasma membraneintrinsic protein 1c2303hexokinase (ATHXK2)2304calcium-dependent proteinkinase23055′-adenylylphosphosulfatereductase, putative2306Erd1 protein precursor(sp|P42762)2307putative protein2308putative protein2309unknown protein2310BCS 1 protein-like protein2311putative protein2312putative protein2313putative protein kinase2314indoleacetic acid (IAA)-induciblegene (IAA7)2315ATP-dependent Clp proteaseregulatory subunit CLPX2316DNA-binding protein RAV12317putative protein2318hypothetical protein2319unknown protein2320unknown protein2321putative protein2322putative thioredoxin reductase2323unknown protein2324putative lectin2325No function assigned by TIGR2326beta-fructosidase2327chlorophyll a/b-binding proteinCP292328photosystem I subunit PSI-E - likeprotein2329peroxidase ATP8a2330putative fructose bisphosphatealdolase2331zinc finger protein ATZF1,putative2332DegP protease precursor2333transcription factor-like protein2334calcium-dependent protein kinase2335hypothetical protein2336putative protein2337glucose-1-phosphateadenylyltransferase (APL3)2338No function assigned by TIGR2339putative Eukaryotic initiation factor4A2340No function assigned by TIGR2341unknown protein2342beta tubulin 1, putative2343one helix protein (OHP)2344No function assigned by TJGR2345zinc finger protein 5, ZFP52346putative MYB family transcriptionfactor2347putative amino acid transporterprotein2348putative potassiumtransporter2349protein kinase (AFC2)2350putative protein2351No function assigned byTIGR2352putative ubiquitin-conjugating enzyme E22353unknown protein2354cytochrome P450monooxygenase (CYP71B3)2355putative myrosinase-binding protein2356putative vacuolar sortingreceptor2357uridine diphosphate glucoseepimerase2358shaggy related proteinkinase, ASK-GAMMA2359ankyrin repeat proteinEMB5062360putative beta-alanine-pyruvate aminotransferase2361putative alcoholdehydrogenase2362putative receptor-likeprotein kinase2363unknown protein2364putative methylmalonatesemi-aldehydedehydrogenase2365hypothetical protein2366unknown protein2367peroxidase ATP13a2368putative glutathioneperoxidase2369squamosa promoter bindingprotein-like 72370photosystem II corecomplex protein, putative2371snoRNA2372photosystem I subunit Xprecursor2373MYB transcription factor(Atmyb2)2374putative PHD-type zinc fingerprotein2375nuclear RNA binding protein A-like protein2376unknown protein2377unknown protein2378unknown protein2379putative amino-cyclopropane-carboxylic acid oxidase (ACCoxidase)2380hypothetical protein2381indole-3-acetate beta-glucosyltransferase like protein2382predicted protein2383unknown protein2384No function assigned by TIGR2385putative photo system I reactioncenter subunit IV2386putative homeodomaintranscription factor2387putative purple acid phosphataseprecursor2388No function assigned by TIGR2389nitrate reductase 1 (NR1)2390putative casein kinase II betasubunit2391pEARLI 1-like protein2392putative protein2393No function assigned by TIGR2394unknown protein2395putative cell wall-plasmamembrane disconnecting CLCTprotein (AIR1A)2396unknown protein2397scarecrow-like 11 - like2398putative anthocyanidin synthase2399putative AP2 domain transcriptionfactor2400caffeoyl-CoA O-methyltransferase -like protein2401unknown protein2402putative protein kinase2403cytochrome P450 -like protein2404putative MADS-box protein ANR12405putative glutathione S-transferase2406hypothetical protein2407similar to gibberellin-regulated proteins2408unknown protein2409putative sensorytransduction histidinekinase2410similar to lateembryogenesis abundantproteins2411unknown protein2412putative protein2413putative ATP-dependentRNA helicase2414putative protein2415putative sucrose synthetase2416beta-fructofuranosidase 12417putative indole-3-acetatebeta-glucosyltransferase2418hypothetical protein2419DNA-directed RNApolymerase II, third largest subunit2420putative transcription factor2421homeobox-leucine zipperprotein ATHB-5 (HD-zip proteinATHB-5) (sp|P46667)2422putative ftsH chloroplastprotease2423replication protein Al - like2424hypothetical protein2425unknown protein2426unknown protein2427putative methionineaminopeptidase2428unknown protein2429fatty acid elongase - likeprotein (cer2-like)2430unknown protein2431putative disease resistanceresponse protein2432putative protein2433unknown protein2434putative protein2435putative protein2436unknown protein2437putative protein2438unknown protein2439unknown protein2440putative protein2441No function assigned by TJGR2442MADS-box protein AGL142443No function assigned by TIGR2444peptidyiprolyl isomerase2445putative s-adenosylmethioninesynthetase2446peroxidase2447ferrochelatase-I2448putative eukaryotic initiation factor4, eIF42449drought-inducible cysteineproteinase RD21A precursor -likeprotein2450unknown protein2451unknown protein2452No function assigned by TIGR2453No function assigned by TIGR2454salt-inducible like protein2455glucose-6-phosphate 1-dehydrogenase24563-hydroxy-3-methylglutaryl CoAreductase (AA 1-592)2457hypothetical protein2458putative protein2459putative putative 60S ribosomalprotein L172460putative inorganic pyrophosphatase2461putative gamma-glutamyltransferase2462heat shock transcription factor -like protein2463mitochondrial chaperonin hsp602464unknown protein2465putative zinc finger proteinidentical to T10M13.222466putative uridylyl transferase2467nodulin-like protein2468putative B-box zinc finger protein2469No function assigned by TIGR2470putative metalloproteinase2471putative cellular apoptosissusceptibility protein2472hypothetical protein2473hypothetical protein2474scarecrow-like 13 (SCL13)2475putative nucleosidetriphosphatase2476unknown protein2477No function assigned byTIGR2478hypothetical protein2479putative phospholipase2480putative snRNP protein2481putative protein2482putative lipase2483putative nonsense-mediatedmRNA decay protein2484No function assigned byTIGR2485protochiorophyllidereductase precursor2486No function assigned byTIGR2487trehalose-6-phosphatesynthase, putative2488unknown protein2489germin-like protein2490plastid protein2491putative protein2492hypothetical protein2493unknown protein2494unknown protein2495histone deacetylase-likeprotein2496unknown protein2497unknown protein2498putative protein2499putative protein2500No function assigned byTIGR2501putative zinc transporterZIP2 - like2502unknown protein2503putative ribosomal-proteinS6 kinase (ATPK19)2504unknown protein2505unknown protein250660S ribosomal protein L10A2507putative protein2508receptor protein kinase (IRK1),putative2509putative nematode-resistanceprotein2510tubulin alpha-5 chain-like protein2511putative DNA-binding protein2512unknown protein2513putative RGA1, giberellin repsonsemodulation protein2514non phototropic hypocotyl 1-like2515RING-H2 finger protein RHA1b2516putative myb-protein2517hydroperoxide lyase (HPOL) likeprotein2518serine/threonine-protein kinase,PK72519putative vacuolar proton-ATPasesubunit2520putative polygalacturonase2521putative ribosomal protein L82522putative adenylate kinase2523germin-like protein (GLP10)2524putative chlorophyll a/b bindingprotein2525chloroplast single subunit DNA-dependent RNA polymerase2526putative protein2527hypothetical protein2528hypothetical protein2529b-keto acyl reductase, putative2530cellulose synthase catalytic subunit2531putative 1-aminocyclopropane-1-carboxylate oxidase2532S-linalool synthase, putative2533phosphoribosyl-ATPpyrophosphohydrolase (At-IE)2534disease resistance RPP5 likeprotein (fragment)2535putative protein2536beta-galactosidase like protein2537putative translationinitiation factor eIF-2, gamma subunit2538ankyrin like protein2539histone H2A- like protein2540putative protein2541salt-tolerance zinc fingerprotein2542unknown protein2543putative protein2544fructose-bisphosphatealdolase2545peroxidase(emb|CAA66964.1)2546patatin-like protein2547salt-inducible proteinhomolog2548hypothetical protein2549xyloglucan endo-transglycosylase-likeprotein2550trihelix DNA-bindingprotein (GT2)2551ubiquitin-conjugatingenzyme 16, putative2552homeobox protein2553envelope Ca2+-ATPase2554snap25a2555putative annexin2556putative protein2557homeodomain transcriptionfactor (ATHB-14)2558heat shock protein, putative2559peroxidase ATP23a2560p68 RNA helicase, putative2561potassium transporter,putative2562putative eukaryotictranslation initiation factor 2 alphasubunit, eIF22563hypothetical protein2564camitine racemase likeprotein2565No function assigned byTIGR2566unknown protein2567unknown protein2568unknown protein2569serine/threonine kinase - likeprotein2570peroxidase (emb|CAA66960.1)2571putative protein2572hypothetical protein2573glycine-rich protein 2 (GRP2)2574unknown protein2575berberine bridge enzyme-likeprotein2576unknown protein2577putative WD-repeat protein2578serine/threonine kinase - likeprotein2579serine/threonine kinase - likeprotein2580Cu2+-transporting ATPase-likeprotein2581translation initiation factor eIF4E2582O-methyltransferase - like protein2583translation initiation factor eIF3 -like protein2584No function assigned by TIGR2585unknown protein2586hypothetical protein2587unknown protein2588unknown protein2589glycine-rich protein like2590putative disease resistance protein2591putative Na+/Ca2+ antiporter2592putative hydroxymethylglutaryl-CoA lyase2593putativephosphoribosylaminoimidazolecarboxylase2594SAR DNA-binding protein - like2595response regulator, putative2596fibrillin precursor-like protein2597beta-ketoacyl-CoA synthase(FIDDLEHEAD)2598lectin like protein2599No function assigned by TIGR2600acidic endochitinase(dbj|BAA21861.1)2601unknown protein2602hypothetical protein2603predicted 0R23 protein ofunknown function2604putative protein2605hypothetical protein2606glycerol-3 -phosphatedehydrogenase2607hypothetical protein2608tat-binding protein, putative2609putative protein2610putative trehalose-6-phosphate phosphatase2611hypothetical protein2612putative flavonol 3-0-glucosyltransferase261360S ribosomal protein L302614putative auxin-inducedprotein2615putative nonspecific lipid-transfer protein precursor2616AtRer1A2617putative aquaporin(tonoplast intrinsic proteingamma)2618hypothetical protein2619putative alanine acetyltransferase2620putative NADP-dependentglyceraldehyde-3-phosphate dehydrogenase2621putative DNA bindingprotein2622putative cystathioninegamma-synthase2623unknown protein2624malate oxidoreductase(malic enzyme)2625unknown protein2626cyclic nucleotide-gatedcation channel2627glyoxalase II, putative2628putative trypsin inhibitor2629unknown protein2630unknown protein2631unknown protein2632nucleosome assembly protein I-likeprotein2633membrane channel like protein2634anthocyanin2, putative2635TWIN SISTER OF FT (TSF)2636putative myb-related transcriptionfactor2637hypothetical protein2638putative RING zinc finger protein2639amino acid transport protein AAT12640putative protein2641putative protein2642xanthine dehydrogenase2643xanthine dehydrogenase - likeprotein2644receptor protein kinase (IRK1),putative2645dehydrin-like protein2646unknown protein2647aldehyde dehydrogenase homolog,putative2648Ran binding protein (AtRanBPlb)2649putative squamosa-promoterbinding protein2650putative protein2651kinesin like protein2652putative cellulose synthase2653calmodulin (cam2)2654fibrillarin - like protein2655putative transmembrane proteinG5p2656putative peroxidase2657putative SNF 1-related proteinkinase2658glutathione 5-transferase, putative2659unknown protein2660hypothetical protein2661putative protein2662phosphatidylinositol-4-phosphate5-kinase isolog2663putative tyrosine decarboxylase2664unknown protein2665SGP1 monomeric G-protein(emb|CAB54517.1)2666putative serinecarboxypeptidase II2667putative L5 ribosomalprotein2668putative glucosyltransferase2669flavonoid 3,5-hydroxylaselike protein2670putative protein2671putative protein2672putative Fe(II)/ascorbateoxidase2673putative anthocyanin 5-aromatic acyltransferase2674casein kinase I2675putative 2,3-bisphosphoglycerate-independentphosphoglycerate mutase2676putative glutathione S-transferase TSI-12677ATP-dependent RNAhelicase2678putative cytochrome P4502679putative WD-40 repeatprotein2680No function assigned byTIGR2681No function assigned byTIGR2682putative protein2683putative extensin2684nodulin-26 - like protein2685RNA helicase(emb|CAA09212.1)2686predicted protein ofunknown function2687putative berberine bridgeenzyme2688thioredoxin, putative2689putative serinecarboxypeptidase I2690cytoclirome P450-likeprotein2691putative pyrophosphate-dependentphosphofructokinase alpha subunit2692putative flavonolglucosyltransferase2693peroxidase ATP20a(emb|CAA67338.1)2694TOPP8 serine/threonine proteinphosphatase type one2695auxin regulated protein IAA18,putative2696putative WRKY-type DNA bindingprotein2697putative glucan synthase2698squalene monooxygenase2699putative proline-rich protein2700G2484-1 protein2701heat shock protein 70 like protein2702unknown protein2703unknown protein

[0205]

2

TABLE 2

ABIOTIC STRESS RESPONSIVE GENE REGULATORY SEQUENCES

REGULATORY

REGULATORY

REGULATORY

SEQ ID NO:
REGION
SEQ ID NO:
REGION
SEQ ID NO:
REGION

1
2704
902
3598
1803
NONE

2
2705
903
3599
1804
4491

3
2706
904
3600
1805
4492

4
2707
905
3601
1806
4493

5
2708
906
3602
1807
4494

6
2709
907
3603
1808
4495

7
2710
908
3604
1809
4496

8
2711
909
3605
1810
4497

9
2712
910
3606
1811
4498

10
2713
911
3607
1812
4499

11
2714
912
3608
1813
4500

12
2715
913
3609
1814
4501

13
2716
914
3610
1815
4502

14
2717
915
3611
1816
4503

15
2718
916
3612
1817
4504

16
2719
917
3613
1818
4505

17
2720
918
3614
1819
4506

18
2721
919
3615
1820
4507

19
2722
920
3616
1821
4508

20
2723
921
3617
1822
4509

21
2724
922
3618
1823
4510

22
2725
923
3619
1824
4511

23
2726
924
3620
1825
4512

24
2727
925
3621
1826
4513

25
2728
926
3622
1827
4514

26
2729
927
3623
1828
4515

27
2730
928
3624
1829
4516

28
2731
929
3625
1830
4517

29
2732
930
3626
1831
4518

30
2733
931
3627
1832
4519

31
2734
932
3628
1833
4520

32
2735
933
3629
1834
4521

33
2736
934
3630
1835
4522

34
2737
935
NONE
1836
4523

35
2738
936
3631
1837
4524

36
2739
937
3632
1838
4525

37
2740
938
3633
1839
4526

38
2741
939
3634
1840
4527

39
2742
940
3635
1841
4528

40
2743
941
3636
1842
4529

41
2744
942
3637
1843
4530

42
2745
943
3638
1844
4531

43
NONE
944
3639
1845
4532

44
2746
945
3640
1846
4533

45
2747
946
3641
1847
4534

46
2748
947
3642
1848
4535

47
2749
948
3643
1849
4536

48
2750
949
3644
1850
4537

49
2751
950
3645
1851
4538

50
2752
951
3646
1852
4539

51
2753
952
3647
1853
4540

52
2754
953
3648
1854
4541

53
2755
954
3649
1855
4542

54
2756
955
3650
1856
4543

55
2757
956
3651
1857
4544

56
2758
957
3652
1858
4545

57
2759
958
3653
1859
4546

58
2760
959
3654
1860
4547

59
2761
960
3655
1861
4548

60
2762
961
3656
1862
4549

61
2763
962
3657
1863
4550

62
2764
963
3658
1864
4551

63
2765
964
3659
1865
4552

64
2766
965
3660
1866
4553

65
2767
966
3661
1867
4554

66
2768
967
3662
1868
4555

67
2769
968
3663
1869
4556

68
2770
969
3664
1870
4557

69
NONE
970
3665
1871
4558

70
2771
971
3666
1872
4559

71
2772
972
3667
1873
4560

72
2773
973
3668
1874
4561

73
2774
974
3669
1875
4562

74
2775
975
3670
1876
4563

75
2776
976
3671
1877
4564

76
2777
977
3672
1878
4565

77
2778
978
3673
1879
4566

78
2779
979
3674
1880
4567

79
2780
980
3675
1881
4568

80
2781
981
3676
1882
4569

81
2782
982
3677
1883
4570

82
2783
983
3678
1884
4571

83
2784
984
3679
1885
4572

84
2785
985
3680
1886
4573

85
2786
986
3681
1887
4574

86
2787
987
3682
1888
4575

87
2788
988
3683
1889
4576

88
2789
989
3684
1890
4577

89
2790
990
3685
1891
4578

90
2791
991
3686
1892
4579

91
2792
992
3687
1893
4580

92
2793
993
3688
1894
4581

93
2794
994
3689
1895
4582

94
2795
995
3690
1896
4583

95
2796
996
3691
1897
NONE

96
2797
997
3692
1898
4584

97
2798
998
3693
1899
4585

98
2799
999
3694
1900
4586

99
2800
1000
3695
1901
4587

100
2801
1001
3696
1902
4588

101
2802
1002
3697
1903
4589

102
2803
1003
3698
1904
4590

103
2804
1004
3699
1905
4591

104
2805
1005
3700
1906
4592

105
2806
1006
3701
1907
NONE

106
2807
1007
3702
1908
4593

107
2808
1008
3703
1909
4594

108
2809
1009
3704
1910
4595

109
2810
1010
3705
1911
4596

110
2811
1011
3706
1912
4597

111
2812
1012
3707
1913
4598

112
2813
1013
3708
1914
4599

113
2814
1014
3709
1915
4600

114
2815
1015
3710
1916
4601

115
2816
1016
3711
1917
4602

116
2817
1017
3712
1918
4603

117
2818
1018
3713
1919
4604

118
2819
1019
3714
1920
4605

119
2820
1020
3715
1921
4606

120
2821
1021
3716
1922
4607

121
2822
1022
3717
1923
4608

122
2823
1023
3718
1924
4609

123
2824
1024
3719
1925
4610

124
2825
1025
3720
1926
4611

125
2826
1026
3721
1927
4612

126
2827
1027
3722
1928
4613

127
2828
1028
3723
1929
4614

128
2829
1029
3724
1930
4615

129
2830
1030
3725
1931
4616

130
2831
1031
3726
1932
4617

131
2832
1032
3727
1933
4618

132
2833
1033
3728
1934
4619

133
2834
1034
3729
1935
4620

134
2835
1035
3730
1936
4621

135
2836
1036
3731
1937
4622

136
2837
1037
3732
1938
4623

137
2838
1038
3733
1939
4624

138
2839
1039
3734
1940
4625

139
2840
1040
3735
1941
4626

140
2841
1041
3736
1942
4627

141
2842
1042
3737
1943
4628

142
2843
1043
3738
1944
4629

143
2844
1044
3739
1945
4630

144
NONE
1045
3740
1946
4631

145
2845
1046
3741
1947
4632

146
2846
1047
3742
1948
4633

147
2847
1048
3743
1949
4634

148
2848
1049
3744
1950
4635

149
2849
1050
3745
1951
4636

150
2850
1051
3746
1952
4637

151
2851
1052
3747
1953
4638

152
2852
1053
3748
1954
4639

153
2853
1054
3749
1955
4640

154
2854
1055
3750
1956
4641

155
2855
1056
3751
1957
4642

156
2856
1057
3752
1958
4643

157
2857
1058
3753
1959
4644

158
2858
1059
3754
1960
4645

159
2859
1060
3755
1961
4646

160
2860
1061
3756
1962
4647

161
2861
1062
3757
1963
4648

162
2862
1063
3758
1964
4649

163
2863
1064
3759
1965
4650

164
2864
1065
3760
1966
4651

165
2865
1066
3761
1967
4652

166
2866
1067
3762
1968
4653

167
2867
1068
3763
1969
4654

168
2868
1069
3764
1970
4655

169
2869
1070
3765
1971
4656

170
2870
1071
3766
1972
4657

171
2871
1072
3767
1973
4658

172
2872
1073
3768
1974
4659

173
2873
1074
3769
1975
4660

174
2874
1075
3770
1976
4661

175
2875
1076
3771
1977
4662

176
2876
1077
3772
1978
4663

177
2877
1078
3773
1979
4664

178
2878
1079
3774
1980
4665

179
2879
1080
3775
1981
4666

180
2880
1081
3776
1982
4667

181
2881
1082
3777
1983
4668

182
2882
1083
3778
1984
4669

183
2883
1084
3779
1985
4670

184
2884
1085
3780
1986
4671

185
2885
1086
3781
1987
4672

186
2886
1087
NONE
1988
4673

187
2887
1088
3782
1989
4674

188
2888
1089
3783
1990
4675

189
2889
1090
3784
1991
4676

190
2890
1091
3785
1992
4677

191
2891
1092
3786
1993
4678

192
2892
1093
3787
1994
4679

193
2893
1094
3788
1995
4680

194
2894
1095
3789
1996
4681

195
2895
1096
3790
1997
4682

196
2896
1097
3791
1998
4683

197
2897
1098
3792
1999
4684

198
2898
1099
3793
2000
4685

199
2899
1100
3794
2001
4686

200
2900
1101
3795
2002
4687

201
2901
1102
3796
2003
4688

202
2902
1103
3797
2004
4689

203
2903
1104
3798
2005
4690

204
2904
1105
3799
2006
4691

205
2905
1106
3800
2007
4692

206
2906
1107
3801
2008
4693

207
2907
1108
3802
2009
4694

208
2908
1109
3803
2010
4695

209
2909
1110
3804
2011
4696

210
2910
1111
3805
2012
4697

211
2911
1112
3806
2013
4698

212
2912
1113
3807
2014
4699

213
2913
1114
3808
2015
4700

214
2914
1115
3809
2016
4701

215
2915
1116
3810
2017
4702

216
2916
1117
3811
2018
4703

217
2917
1118
3812
2019
4704

218
2918
1119
3813
2020
4705

219
2919
1120
3814
2021
4706

220
2920
1121
3815
2022
4707

221
2921
1122
3816
2023
4708

222
2922
1123
3817
2024
4709

223
2923
1124
3818
2025
4710

224
2924
1125
3819
2026
4711

225
2925
1126
3820
2027
4712

226
2926
1127
3821
2028
4713

227
2927
1128
3822
2029
4714

228
2928
1129
3823
2030
NONE

229
2929
1130
3824
2031
4715

230
2930
1131
3825
2032
4716

231
2931
1132
3826
2033
4717

232
2932
1133
3827
2034
4718

233
2933
1134
3828
2035
4719

234
2934
1135
3829
2036
4720

235
2935
1136
3830
2037
4721

236
2936
1137
3831
2038
4722

237
2937
1138
3832
2039
4723

238
2938
1139
3833
2040
4724

239
2939
1140
3834
2041
4725

240
2940
1141
3835
2042
4726

241
2941
1142
3836
2043
4727

242
2942
1143
3837
2044
4728

243
2943
1144
3838
2045
4729

244
2944
1145
3839
2046
4730

245
2945
1146
3840
2047
4731

246
2946
1147
3841
2048
4732

247
2947
1148
3842
2049
4733

248
2948
1149
3843
2050
4734

249
2949
1150
3844
2051
4735

250
2950
1151
3845
2052
4736

251
2951
1152
3846
2053
4737

252
2952
1153
3847
2054
4738

253
2953
1154
3848
2055
4739

254
2954
1155
3849
2056
4740

255
2955
1156
3850
2057
4741

256
2956
1157
3851
2058
4742

257
2957
1158
3852
2059
4743

258
2958
1159
3853
2060
4744

259
2959
1160
3854
2061
4745

260
2960
1161
3855
2062
4746

261
2961
1162
3856
2063
4747

262
2962
1163
3857
2064
4748

263
2963
1164
3858
2065
4749

264
2964
1165
3859
2066
4750

265
2965
1166
3860
2067
4751

266
2966
1167
3861
2068
4752

267
2967
1168
3862
2069
4753

268
2968
1169
3863
2070
4754

269
2969
1170
3864
2071
4755

270
2970
1171
3865
2072
4756

271
2971
1172
3866
2073
4757

272
2972
1173
3867
2074
4758

273
2973
1174
3868
2075
4759

274
2974
1175
3869
2076
4760

275
2975
1176
3870
2077
4761

276
2976
1177
3871
2078
4762

277
2977
1178
3872
2079
4763

278
2978
1179
3873
2080
4764

279
2979
1180
3874
2081
4765

280
2980
1181
3875
2082
4766

281
2981
1182
3876
2083
4767

282
2982
1183
3877
2084
4768

283
2983
1184
3878
2085
4769

284
2984
1185
3879
2086
4770

285
2985
1186
3880
2087
4771

286
2986
1187
3881
2088
4772

287
2987
1188
3882
2089
4773

288
2988
1189
3883
2090
4774

289
2989
1190
3884
2091
4775

290
2990
1191
3885
2092
4776

291
2991
1192
3886
2093
4777

292
2992
1193
3887
2094
4778

293
2993
1194
3888
2095
4779

294
2994
1195
3889
2096
4780

295
2995
1196
3890
2097
4781

296
2996
1197
3891
2098
4782

297
2997
1198
3892
2099
4783

298
2998
1199
3893
2100
4784

299
2999
1200
3894
2101
4785

300
3000
1201
3895
2102
4786

301
3001
1202
3896
2103
4787

302
3002
1203
3897
2104
4788

303
3003
1204
3898
2105
4789

304
NONE
1205
3899
2106
4790

305
3004
1206
3900
2107
4791

306
3005
1207
3901
2108
4792

307
3006
1208
3902
2109
4793

308
3007
1209
3903
2110
4794

309
3008
1210
3904
2111
4795

310
3009
1211
3905
2112
4796

311
3010
1212
3906
2113
4797

312
3011
1213
3907
2114
4798

313
3012
1214
3908
2115
4799

314
3013
1215
3909
2116
4800

315
3014
1216
3910
2117
4801

316
3015
1217
3911
2118
4802

317
3016
1218
3912
2119
4803

318
3017
1219
3913
2120
4804

319
3018
1220
3914
2121
4805

320
3019
1221
3915
2122
4806

321
3020
1222
3916
2123
4807

322
3021
1223
3917
2124
4808

323
3022
1224
3918
2125
4809

324
3023
1225
3919
2126
4810

325
3024
1226
3920
2127
4811

326
3025
1227
3921
2128
4812

327
3026
1228
3922
2129
4813

328
3027
1229
3923
2130
4814

329
3028
1230
3924
2131
4815

330
3029
1231
3925
2132
4816

331
3030
1232
3926
2133
4817

332
3031
1233
3927
2134
4818

333
3032
1234
3928
2135
4819

334
3033
1235
3929
2136
4820

335
3034
1236
3930
2137
4821

336
3035
1237
3931
2138
4822

337
3036
1238
3932
2139
4823

338
3037
1239
3933
2140
4824

339
3038
1240
3934
2141
4825

340
3039
1241
3935
2142
4826

341
3040
1242
3936
2143
4827

342
3041
1243
3937
2144
4828

343
3042
1244
3938
2145
4829

344
3043
1245
3939
2146
4830

345
3044
1246
3940
2147
4831

346
3045
1247
3941
2148
4832

347
3046
1248
3942
2149
4833

348
3047
1249
3943
2150
4834

349
3048
1250
3944
2151
NONE

350
3049
1251
3945
2152
4835

351
3050
1252
3946
2153
4836

352
3051
1253
3947
2154
4837

353
3052
1254
3948
2155
4838

354
3053
1255
3949
2156
4839

355
3054
1256
3950
2157
4840

356
3055
1257
3951
2158
4841

357
3056
1258
3952
2159
4842

358
3057
1259
3953
2160
4843

359
3058
1260
3954
2161
4844

360
3059
1261
3955
2162
4845

361
3060
1262
3956
2163
4846

362
3061
1263
3957
2164
4847

363
3062
1264
3958
2165
4848

364
3063
1265
3959
2166
4849

365
3064
1266
3960
2167
4850

366
3065
1267
3961
2168
4851

367
3066
1268
3962
2169
4852

368
3067
1269
3963
2170
4853

369
3068
1270
3964
2171
4854

370
3069
1271
3965
2172
4855

371
3070
1272
3966
2173
4856

372
3071
1273
3967
2174
4857

373
3072
1274
3968
2175
4858

374
3073
1275
3969
2176
4859

375
3074
1276
3970
2177
4860

376
3075
1277
3971
2178
4861

377
3076
1278
3972
2179
4862

378
3077
1279
3973
2180
4863

379
3078
1280
3974
2181
4864

380
3079
1281
3975
2182
4865

381
3080
1282
3976
2183
4866

382
3081
1283
3977
2184
4867

383
3082
1284
3978
2185
4868

384
3083
1285
3979
2186
4869

385
3084
1286
3980
2187
4870

386
3085
1287
3981
2188
4871

387
3086
1288
3982
2189
4872

388
3087
1289
3983
2190
4873

389
3088
1290
3984
2191
4874

390
3089
1291
3985
2192
4875

391
3090
1292
3986
2193
4876

392
3091
1293
3987
2194
4877

393
3092
1294
3988
2195
4878

394
3093
1295
3989
2196
4879

395
3094
1296
3990
2197
4880

396
3095
1297
3991
2198
4881

397
3096
1298
3992
2199
4882

398
3097
1299
3993
2200
4883

399
3098
1300
3994
2201
4884

400
3099
1301
3995
2202
4885

401
3100
1302
3996
2203
4886

402
3101
1303
3997
2204
4887

403
3102
1304
3998
2205
4888

404
3103
1305
3999
2206
4889

405
3104
1306
4000
2207
4890

406
3105
1307
4001
2208
4891

407
3106
1308
4002
2209
4892

408
3107
1309
4003
2210
4893

409
3108
1310
4004
2211
4894

410
3109
1311
4005
2212
4895

411
3110
1312
4006
2213
4896

412
3111
1313
4007
2214
4897

413
3112
1314
4008
2215
4898

414
3113
1315
4009
2216
4899

415
3114
1316
4010
2217
4900

416
3115
1317
4011
2218
4901

417
3116
1318
4012
2219
4902

418
3117
1319
4013
2220
4903

419
3118
1320
4014
2221
4904

420
3119
1321
4015
2222
4905

421
3120
1322
4016
2223
4906

422
3121
1323
4017
2224
4907

423
3122
1324
4018
2225
4908

424
3123
1325
4019
2226
4909

425
3124
1326
4020
2227
4910

426
3125
1327
4021
2228
4911

427
3126
1328
4022
2229
4912

428
3127
1329
4023
2230
4913

429
3128
1330
NONE
2231
4914

430
3129
1331
4024
2232
4915

431
3130
1332
4025
2233
4916

432
3131
1333
4026
2234
4917

433
3132
1334
4027
2235
4918

434
3133
1335
4028
2236
4919

435
3134
1336
4029
2237
4920

436
3135
1337
4030
2238
4921

437
3136
1338
4031
2239
4922

438
3137
1339
4032
2240
4923

439
3138
1340
4033
2241
4924

440
3139
1341
4034
2242
4925

441
3140
1342
4035
2243
4926

442
3141
1343
4036
2244
4927

443
3142
1344
4037
2245
4928

444
3143
1345
4038
2246
4929

445
3144
1346
4039
2247
4930

446
3145
1347
4040
2248
NONE

447
3146
1348
4041
2249
4931

448
3147
1349
4042
2250
4932

449
3148
1350
4043
2251
4933

450
3149
1351
4044
2252
4934

451
3150
1352
4045
2253
4935

452
3151
1353
4046
2254
4936

453
3152
1354
4047
2255
4937

454
3153
1355
4048
2256
4938

455
3154
1356
4049
2257
4939

456
3155
1357
4050
2258
4940

457
3156
1358
4051
2259
4941

458
3157
1359
4052
2260
4942

459
3158
1360
4053
2261
4943

460
3159
1361
4054
2262
4944

461
3160
1362
4055
2263
4945

462
3161
1363
4056
2264
4946

463
3162
1364
4057
2265
4947

464
3163
1365
4058
2266
4948

465
3164
1366
4059
2267
4949

466
3165
1367
4060
2268
4950

467
3166
1368
4061
2269
4951

468
3167
1369
4062
2270
4952

469
3168
1370
4063
2271
4953

470
3169
1371
4064
2272
4954

471
3170
1372
4065
2273
4955

472
3171
1373
4066
2274
4956

473
3172
1374
4067
2275
4957

474
3173
1375
4068
2276
4958

475
3174
1376
4069
2277
4959

476
3175
1377
4070
2278
4960

477
3176
1378
4071
2279
4961

478
3177
1379
4072
2280
4962

479
3178
1380
4073
2281
4963

480
3179
1381
4074
2282
4964

481
3180
1382
4075
2283
4965

482
3181
1383
4076
2284
4966

483
3182
1384
4077
2285
4967

484
3183
1385
4078
2286
4968

485
3184
1386
4079
2287
4969

486
3185
1387
4080
2288
4970

487
3186
1388
4081
2289
4971

488
3187
1389
4082
2290
4972

489
3188
1390
4083
2291
4973

490
3189
1391
4084
2292
4974

491
3190
1392
4085
2293
4975

492
3191
1393
4086
2294
4976

493
3192
1394
4087
2295
4977

494
3193
1395
4088
2296
4978

495
3194
1396
4089
2297
4979

496
3195
1397
4090
2298
4980

497
3196
1398
4091
2299
4981

498
3197
1399
4092
2300
4982

499
3198
1400
4093
2301
4983

500
3199
1401
4094
2302
4984

501
3200
1402
4095
2303
4985

502
3201
1403
4096
2304
4986

503
3202
1404
4097
2305
4987

504
3203
1405
4098
2306
4988

505
3204
1406
4099
2307
4989

506
3205
1407
4100
2308
4990

507
3206
1408
4101
2309
4991

508
3207
1409
4102
2310
4992

509
3208
1410
4103
2311
4993

510
3209
1411
4104
2312
4994

511
3210
1412
4105
2313
4995

512
3211
1413
4106
2314
4996

513
3212
1414
4107
2315
4997

514
3213
1415
4108
2316
4998

515
3214
1416
4109
2317
4999

516
3215
1417
4110
2318
5000

517
3216
1418
4111
2319
5001

518
3217
1419
4112
2320
5002

519
3218
1420
4113
2321
5003

520
3219
1421
4114
2322
5004

521
3220
1422
4115
2323
5005

522
3221
1423
4116
2324
5006

523
3222
1424
4117
2325
5007

524
3223
1425
4118
2326
5008

525
3224
1426
4119
2327
5009

526
3225
1427
4120
2328
5010

527
3226
1428
4121
2329
5011

528
3227
1429
4122
2330
5012

529
3228
1430
4123
2331
5013

530
3229
1431
4124
2332
5014

531
3230
1432
NONE
2333
5015

532
3231
1433
4125
2334
5016

533
3232
1434
4126
2335
5017

534
3233
1435
4127
2336
5018

535
3234
1436
4128
2337
5019

536
3235
1437
4129
2338
5020

537
3236
1438
4130
2339
5021

538
3237
1439
4131
2340
NONE

539
3238
1440
4132
2341
5022

540
3239
1441
4133
2342
5023

541
3240
1442
4134
2343
5024

542
3241
1443
4135
2344
5025

543
3242
1444
4136
2345
5026

544
3243
1445
4137
2346
5027

545
3244
1446
4138
2347
5028

546
3245
1447
4139
2348
5029

547
3246
1448
4140
2349
5030

548
3247
1449
4141
2350
5031

549
3248
1450
4142
2351
5032

550
3249
1451
4143
2352
5033

551
3250
1452
4144
2353
5034

552
3251
1453
4145
2354
5035

553
3252
1454
4146
2355
5036

554
3253
1455
4147
2356
5037

555
3254
1456
4148
2357
5038

556
3255
1457
4149
2358
5039

557
3256
1458
4150
2359
5040

558
3257
1459
4151
2360
5041

559
3258
1460
4152
2361
5042

560
3259
1461
4153
2362
5043

561
3260
1462
4154
2363
5044

562
3261
1463
4155
2364
5045

563
3262
1464
4156
2365
5046

564
3263
1465
4157
2366
5047

565
3264
1466
4158
2367
5048

566
3265
1467
4159
2368
5049

567
3266
1468
4160
2369
5050

568
3267
1469
4161
2370
5051

569
3268
1470
4162
2371
NONE

570
3269
1471
4163
2372
5052

571
3270
1472
4164
2373
5053

572
3271
1473
4165
2374
5054

573
3272
1474
4166
2375
5055

574
3273
1475
4167
2376
5056

575
3274
1476
4168
2377
5057

576
3275
1477
4169
2378
5058

577
3276
1478
4170
2379
5059

578
3277
1479
4171
2380
5060

579
3278
1480
4172
2381
5061

580
3279
1481
4173
2382
5062

581
3280
1482
4174
2383
5063

582
3281
1483
4175
2384
5064

583
3282
1484
4176
2385
5065

584
3283
1485
4177
2386
5066

585
3284
1486
4178
2387
5067

586
3285
1487
4179
2388
5068

587
3286
1488
4180
2389
5069

588
3287
1489
4181
2390
5070

589
3288
1490
4182
2391
5071

590
3289
1491
4183
2392
5072

591
3290
1492
4184
2393
5073

592
3291
1493
4185
2394
5074

593
3292
1494
4186
2395
5075

594
3293
1495
4187
2396
5076

595
3294
1496
4188
2397
5077

596
3295
1497
4189
2398
5078

597
3296
1498
4190
2399
5079

598
3297
1499
4191
2400
5080

599
3298
1500
4192
2401
5081

600
3299
1501
4193
2402
5082

601
3300
1502
4194
2403
5083

602
3301
1503
4195
2404
5084

603
3302
1504
4196
2405
5085

604
3303
1505
4197
2406
5086

605
3304
1506
4198
2407
5087

606
3305
1507
4199
2408
5088

607
3306
1508
4200
2409
5089

608
3307
1509
4201
2410
5090

609
3308
1510
4202
2411
5091

610
3309
1511
4203
2412
5092

611
3310
1512
4204
2413
5093

612
3311
1513
4205
2414
5094

613
3312
1514
4206
2415
5095

614
3313
1515
4207
2416
5096

615
3314
1516
4208
2417
5097

616
3315
1517
4209
2418
5098

617
3316
1518
4210
2419
5099

618
3317
1519
4211
2420
5100

619
3318
1520
4212
2421
5101

620
3319
1521
4213
2422
5102

621
3320
1522
4214
2423
5103

622
3321
1523
4215
2424
5104

623
3322
1524
4216
2425
5105

624
3323
1525
4217
2426
5106

625
3324
1526
4218
2427
5107

626
3325
1527
4219
2428
5108

627
3326
1528
4220
2429
5109

628
3327
1529
4221
2430
5110

629
3328
1530
4222
2431
5111

630
3329
1531
4223
2432
5112

631
3330
1532
4224
2433
5113

632
3331
1533
4225
2434
5114

633
3332
1534
4226
2435
5115

634
3333
1535
4227
2436
5116

635
3334
1536
4228
2437
5117

636
3335
1537
4229
2438
5118

637
3336
1538
4230
2439
5119

638
3337
1539
4231
2440
5120

639
3338
1540
4232
2441
5121

640
3339
1541
4233
2442
5122

641
3340
1542
4234
2443
NONE

642
3341
1543
4235
2444
5123

643
3342
1544
4236
2445
5124

644
3343
1545
4237
2446
5125

645
3344
1546
4238
2447
5126

646
3345
1547
4239
2448
5127

647
3346
1548
4240
2449
5128

648
3347
1549
4241
2450
5129

649
3348
1550
4242
2451
5130

650
3349
1551
4243
2452
5131

651
3350
1552
4244
2453
5132

652
3351
1553
4245
2454
5133

653
3352
1554
4246
2455
5134

654
3353
1555
4247
2456
5135

655
3354
1556
4248
2457
5136

656
3355
1557
4249
2458
5137

657
3356
1558
NONE
2459
5138

658
3357
1559
4250
2460
5139

659
3358
1560
4251
2461
5140

660
3359
1561
4252
2462
5141

661
3360
1562
4253
2463
5142

662
3361
1563
4254
2464
5143

663
3362
1564
4255
2465
5144

664
3363
1565
4256
2466
5145

665
3364
1566
4257
2467
5146

666
3365
1567
4258
2468
5147

667
3366
1568
4259
2469
NONE

668
3367
1569
4260
2470
5148

669
3368
1570
4261
2471
5149

670
3369
1571
4262
2472
5150

671
3370
1572
4263
2473
5151

672
3371
1573
4264
2474
5152

673
3372
1574
4265
2475
5153

674
3373
1575
4266
2476
5154

675
3374
1576
4267
2477
5155

676
3375
1577
4268
2478
5156

677
3376
1578
4269
2479
5157

678
3377
1579
4270
2480
5158

679
3378
1580
4271
2481
5159

680
3379
1581
4272
2482
5160

681
3380
1582
4273
2483
5161

682
3381
1583
4274
2484
5162

683
3382
1584
4275
2485
5163

684
3383
1585
4276
2486
5164

685
3384
1586
4277
2487
5165

686
3385
1587
4278
2488
5166

687
3386
1588
4279
2489
5167

688
3387
1589
4280
2490
5168

689
3388
1590
4281
2491
5169

690
3389
1591
4282
2492
5170

691
3390
1592
4283
2493
5171

692
3391
1593
4284
2494
5172

693
3392
1594
4285
2495
5173

694
3393
1595
4286
2496
5174

695
3394
1596
4287
2497
5175

696
3395
1597
4288
2498
5176

697
3396
1598
4289
2499
5177

698
3397
1599
4290
2500
5178

699
3398
1600
4291
2501
5179

700
3399
1601
4292
2502
5180

701
3400
1602
4293
2503
5181

702
3401
1603
4294
2504
5182

703
3402
1604
4295
2505
5183

704
3403
1605
4296
2506
5184

705
3404
1606
4297
2507
5185

706
3405
1607
4298
2508
5186

707
3406
1608
4299
2509
5187

708
3407
1609
4300
2510
5188

709
3408
1610
4301
2511
5189

710
3409
1611
4302
2512
5190

711
3410
1612
4303
2513
5191

712
3411
1613
4304
2514
5192

713
3412
1614
4305
2515
5193

714
3413
1615
4306
2516
5194

715
3414
1616
4307
2517
5195

716
3415
1617
4308
2518
5196

717
3416
1618
4309
2519
5197

718
3417
1619
4310
2520
5198

719
3418
1620
4311
2521
5199

720
3419
1621
4312
2522
5200

721
3420
1622
4313
2523
5201

722
3421
1623
4314
2524
5202

723
3422
1624
4315
2525
5203

724
3423
1625
4316
2526
5204

725
3424
1626
4317
2527
5205

726
3425
1627
4318
2528
5206

727
3426
1628
4319
2529
5207

728
3427
1629
4320
2530
5208

729
3428
1630
4321
2531
5209

730
3429
1631
4322
2532
5210

731
3430
1632
4323
2533
5211

732
3431
1633
4324
2534
5212

733
3432
1634
4325
2535
5213

734
3433
1635
4326
2536
5214

735
3434
1636
4327
2537
5215

736
3435
1637
4328
2538
5216

737
3436
1638
4329
2539
5217

738
3437
1639
4330
2540
5218

739
3438
1640
4331
2541
5219

740
3439
1641
4332
2542
5220

741
3440
1642
4333
2543
5221

742
3441
1643
4334
2544
5222

743
3442
1644
4335
2545
5223

744
3443
1645
4336
2546
5224

745
3444
1646
4337
2547
5225

746
3445
1647
4338
2548
5226

747
3446
1648
4339
2549
5227

748
3447
1649
4340
2550
5228

749
3448
1650
4341
2551
5229

750
3449
1651
4342
2552
5230

751
3450
1652
4343
2553
5231

752
3451
1653
4344
2554
5232

753
3452
1654
4345
2555
5233

754
3453
1655
4346
2556
5234

755
3454
1656
4347
2557
5235

756
3455
1657
4348
2558
5236

757
3456
1658
4349
2559
5237

758
3457
1659
4350
2560
5238

759
3458
1660
4351
2561
5239

760
3459
1661
4352
2562
5240

761
3460
1662
4353
2563
5241

762
3461
1663
NONE
2564
5242

763
3462
1664
4354
2565
5243

764
3463
1665
4355
2566
5244

765
3464
1666
4356
2567
5245

766
3465
1667
4357
2568
5246

767
3466
1668
4358
2569
5247

768
3467
1669
4359
2570
5248

769
3468
1670
4360
2571
5249

770
3469
1671
4361
2572
5250

771
3470
1672
4362
2573
5251

772
3471
1673
4363
2574
5252

773
3472
1674
4364
2575
5253

774
3473
1675
4365
2576
5254

775
3474
1676
4366
2577
5255

776
3475
1677
4367
2578
5256

777
3476
1678
4368
2579
5257

778
3477
1679
4369
2580
5258

779
3478
1680
4370
2581
5259

780
3479
1681
4371
2582
5260

781
3480
1682
4372
2583
5261

782
3481
1683
4373
2584
5262

783
3482
1684
4374
2585
5263

784
3483
1685
4375
2586
5264

785
3484
1686
4376
2587
5265

786
3485
1687
4377
2588
5266

787
3486
1688
4378
2589
5267

788
3487
1689
4379
2590
5268

789
3488
1690
4380
2591
5269

790
3489
1691
4381
2592
5270

791
3490
1692
4382
2593
5271

792
3491
1693
4383
2594
5272

793
3492
1694
4384
2595
5273

794
3493
1695
4385
2596
5274

795
3494
1696
4386
2597
5275

796
3495
1697
4387
2598
5276

797
3496
1698
4388
2599
NONE

798
3497
1699
4389
2600
5277

799
3498
1700
4390
2601
5278

800
3499
1701
4391
2602
5279

801
3500
1702
4392
2603
5280

802
3501
1703
4393
2604
5281

803
3502
1704
4394
2605
5282

804
3503
1705
4395
2606
5283

805
3504
1706
4396
2607
5284

806
3505
1707
4397
2608
5285

807
3506
1708
4398
2609
5286

808
3507
1709
4399
2610
5287

809
3508
1710
4400
2611
5288

810
3509
1711
4401
2612
5289

811
3510
1712
NONE
2613
5290

812
3511
1713
4402
2614
5291

813
3512
1714
4403
2615
5292

814
3513
1715
4404
2616
5293

815
3514
1716
4405
2617
5294

816
3515
1717
4406
2618
5295

817
3516
1718
4407
2619
5296

818
3517
1719
4408
2620
5297

819
3518
1720
4409
2621
5298

820
3519
1721
4410
2622
5299

821
3520
1722
4411
2623
5300

822
3521
1723
4412
2624
5301

823
3522
1724
4413
2625
5302

824
3523
1725
4414
2626
5303

825
3524
1726
4415
2627
5304

826
3525
1727
4416
2628
5305

827
3526
1728
4417
2629
5306

828
3527
1729
4418
2630
5307

829
3528
1730
4419
2631
5308

830
3529
1731
4420
2632
5309

831
3530
1732
4421
2633
5310

832
3531
1733
4422
2634
5311

833
3532
1734
4423
2635
5312

834
3533
1735
4424
2636
5313

835
3534
1736
4425
2637
5314

836
3535
1737
4426
2638
5315

837
3536
1738
4427
2639
5316

838
3537
1739
4428
2640
5317

839
3538
1740
4429
2641
5318

840
3539
1741
4430
2642
5319

841
3540
1742
4431
2643
5320

842
3541
1743
4432
2644
5321

843
3542
1744
4433
2645
5322

844
3543
1745
4434
2646
5323

845
3544
1746
4435
2647
5324

846
3545
1747
4436
2648
5325

847
3546
1748
4437
2649
5326

848
3547
1749
4438
2650
5327

849
3548
1750
4439
2651
5328

850
3549
1751
4440
2652
5329

851
3550
1752
4441
2653
5330

852
3551
1753
4442
2654
5331

853
3552
1754
4443
2655
5332

854
3553
1755
4444
2656
5333

855
3554
1756
4445
2657
5334

856
3555
1757
4446
2658
5335

857
3556
1758
4447
2659
5336

858
3557
1759
4448
2660
5337

859
3558
1760
4449
2661
5338

860
3559
1761
4450
2662
5339

861
3560
1762
4451
2663
5340

862
3561
1763
4452
2664
5341

863
3562
1764
4453
2665
5342

864
3563
1765
4454
2666
5343

865
3564
1766
4455
2667
5344

866
3565
1767
4456
2668
5345

867
3566
1768
4457
2669
5346

868
3567
1769
4458
2670
5347

869
3568
1770
4459
2671
5348

870
3569
1771
4460
2672
5349

871
3570
1772
4461
2673
5350

872
3571
1773
4462
2674
5351

873
3572
1774
4463
2675
5352

874
3573
1775
4464
2676
5353

875
3574
1776
4465
2677
5354

876
3575
1777
4466
2678
5355

877
3576
1778
4467
2679
5356

878
3577
1779
4468
2680
5357

879
3578
1780
4469
2681
NONE

880
3579
1781
4470
2682
5358

881
3580
1782
4471
2683
5359

882
3581
1783
4472
2684
5360

883
3582
1784
NONE
2685
5361

884
3583
1785
4473
2686
5362

885
3584
1786
4474
2687
5363

886
3585
1787
4475
2688
5364

887
NONE
1788
4476
2689
5365

888
3586
1789
4477
2690
5366

889
3587
1790
4478
2691
5367

890
3588
1791
4479
2692
5368

891
3589
1792
4480
2693
5369

892
3590
1793
4481
2694
5370

893
3591
1794
4482
2695
5371

894
NONE
1795
4483
2696
5372

895
NONE
1796
4484
2697
5373

896
3592
1797
4485
2698
5374

897
3593
1798
4486
2699
5375

898
3594
1799
4487
2700
5376

899
3595
1800
4488
2701
5377

900
3596
1801
4489
2702
5378

901
3597
1802
4490
2703
5379

[0206]

3

TABLE 3

COLD RESPONSIVE SEQUENCES

SEQ

SEQ

SEQ

ID
AFFYMETRIX
ID
AFFYMETRIX
ID
AFFYMETRIX

NO:
ID NO:
NO:
ID NO:
NO:
ID NO:

1
11991_G_AT
406
14566_AT
824
17457_AT

2
11992_AT
407
14572_AT
825
17458_AT

3
11997_AT
408
14579_AT
826
17462_S_AT

4
11998_AT
409
14587_AT
827
17463_AT

5
12001_AT
410
14591_AT
828
17465_AT

6
12006_S_AT
411
14595_AT
829
17466_S_AT

7
12007_AT
412
14602_AT
830
17475_AT

8
12009_AT
413
14603_AT
831
17479_AT

9
12018_AT
414
14605_AT
832
17482_S_AT

10
12022_AT
415
14620_S_AT
833
17495_S_AT

11
12026_AT
416
14626_S_AT
834
17508_S_AT

12
12031_AT
417
14630_S_AT
835
17522_S_AT

13
12047_AT

16559_S_AT
836
17523_S_AT

14
12051_AT
418
14637_S_AT
837
17537_S_AT

15
12052_AT

17122_S_AT
838
17538_S_AT

16
12053_AT
419
14642_F_AT
839
17539_S_AT

17
12060_AT
420
14650_S_AT
840
17546_S_AT

18
12072_AT

15150_S_AT

18694_S_AT

19
12074_AT
421
14654_S_AT
841
17557_S_AT

20
12102_AT
422
14667_S_AT
842
17560_S_AT

21
12112_AT

18299_S_AT
843
17562_AT

22
12117_AT
423
14669_S_AT
844
17564_S_AT

23
12125_AT

16136_S_AT

19361_S_AT

24
12130_AT
424
14672_S_AT
845
17565_S_AT

25
12143_AT
425
14679_S_AT
846
17568_AT

26
12145_S_AT
426
14682_I_AT
847
17573_AT

27
12149_AT
427
14689_AT
848
17577_G_AT

28
12156_AT
428
14697_G_AT
849
17578_AT

29
12163_AT

16902_AT
850
17596_AT

30
12166_I_AT
429
14701_S_AT
851
17627_AT

31
12167_AT

14734_S_AT
852
17631_AT

32
12169_I_AT
430
14703_AT
853
17632_AT

33
12175_AT
431
14711_S_AT
854
17672_AT

34
12176_AT
432
14712_S_AT
855
17675_AT

35
12179_AT

20530_S_AT
856
17677_AT

36
12187_AT
433
14713_S_AT
857
17732_AT

15920_I_AT
434
14715_S_AT
858
17743_AT

37
12195_AT
435
14728_S_AT
859
17748_AT

38
12196_AT
436
14731_S_AT
860
17782_AT

39
12198_AT
437
14781_AT
861
17823_S_AT

40
12200_AT
438
14797_S_AT
862
17841_AT

41
12202_AT
439
14800_AT
863
17849_S_AT

42
12214_G_AT
440
14809_AT
864
17852_G_AT

43
12219_AT
441
14843_AT
865
17857_AT

44
12224_AT
442
14847_AT
866
17865_AT

45
12226_AT
443
14872_AT
867
17882_AT

46
12233_AT
444
14886_AT
868
17885_AT

47
12240_AT
445
14896_AT
869
17900_S_AT

48
12253_G_AT
446
14900_AT
870
17910_AT

49
12256_AT
447
14908_AT
871
17911_AT

50
12269_S_AT
448
14912_AT
872
17916_AT

51
12270_AT
449
14914_AT
873
17917_S_AT

52
12284_AT
450
14942_AT
874
17918_AT

53
12287_S_AT
451
14945_AT
875
17921_S_AT

17570_G_AT
452
14955_AT
876
17922_AT

54
12293_AT
453
14957_S_AT
877
17926_S_AT

55
12294_S_AT
454
14958_AT
878
17933_AT

56
12300_AT
455
14965_AT
879
17935_AT

57
12307_AT
456
14974_AT
880
17956_I_AT

58
12312_AT
457
14980_AT
881
17966_AT

59
12315_AT
458
14981_AT
882
17967_AT

60
12324_I_AT
459
14984_S_AT
883
17970_I_AT

61
12331_S_AT
460
19995_AT
884
17978_S_AT

62
12336_AT
461
15004_AT

20635_S_AT

63
12344_AT
462
15009_AT
885
17986_S_AT

64
12348_AT
463
15010_AT
886
17993_AT

65
12353_AT
464
15024_AT
887
18001_AT

66
12359_S_AT
465
15026_AT
888
18003_AT

67
12372_AT
466
15036_R_AT
889
18004_AT

68
12374_I_AT
467
15054_AT
890
18005_AT

12726_F_AT
468
15056_AT
891
18029_G_AT

69
12390_AT
469
15057_AT

18030_I_AT

70
12395_S_AT
470
15066_AT
892
18040_S_AT

71
12405_AT
471
15073_AT
893
18045_AT

72
12408_AT
472
15081_AT
894
18064_R_AT

73
12410_G_AT
473
15083_AT
895
18065_R_AT

74
12419_AT
474
15091_AT
896
18074_AT

75
12427_AT
475
15097_S_AT
897
18076_S_AT

76
12431_AT
476
15101_S_AT
898
18077_AT

77
12436_AT
477
15102_S_AT
899
18081_AT

78
12438_AT
478
15107_S_AT
900
18154_S_AT

79
12443_S_AT
479
15112_S_AT

18365_S_AT

80
12447_AT
480
15116_F_AT
901
18165_AT

81
12450_S_AT
481
15118_S_AT
902
18174_AT

82
12452_AT
482
15122_S_AT
903
18176_AT

83
12474_AT
483
15130_S_AT
904
18194_I_AT

84
12477_AT
484
15131_S_AT
905
18197_AT

85
12491_AT
485
15132_S_AT
906
18198_AT

86
12497_AT

17585_S_AT
907
18213_AT

87
12500_S_AT
486
15139_S_AT
908
18219_AT

88
12503_AT
487
15143_S_AT
909
18221_AT

89
12515_AT
488
15146_S_AT
910
18222_AT

90
12516_S_AT
489
15159_S_AT
911
18226_S_AT

91
12523_AT

15160_S_AT
912
18232_AT

92
12526_AT
490
15162_S_AT
913
18237_AT

93
12527_AT
491
15167_S_AT
914
18241_AT

94
12532_AT
492
15171_S_AT
915
18257_AT

95
12534_G_AT
493
15174_F_AT
916
18258_S_AT

96
12544_AT
494
15178_S_AT
917
18269_S_AT

97
12549_S_AT
495
15185_S_AT
918
18274_S_AT

98
12550_S_AT

18023_S_AT
919
18275_AT

17103_S_AT
496
15188_S_AT
920
18278_AT

99
12552_AT
497
15193_S_AT
921
18282_AT

100
12555_S_AT
498
15196_S_AT
922
18283_AT

101
12576_S_AT
499
15197_S_AT
923
18290_AT

102
12581_S_AT
500
15201_F_AT
924
18291_AT

16645_S_AT
501
15213_S_AT
925
18306_AT

103
12587_AT
502
15243_AT
926
18316_AT

104
12597_AT
503
15256_AT
927
18317_AT

105
12602_AT
504
15270_AT
928
18327_S_AT

106
12610_AT
505
15319_AT
929
18337_S_AT

107
12631_AT
506
15325_AT
930
18339_AT

108
12646_AT
507
15337_AT
931
18347_S_AT

109
12649_AT
508
15341_AT
932
18383_AT

110
12650_AT
509
15343_AT
933
18390_AT

111
12653_AT
510
15348_AT
934
18439_S_AT

112
12661_AT
511
15350_AT
935
18465_S_AT

113
12666_AT
512
15355_S_AT
936
18487_AT

114
12674_AT
513
15367_AT
937
18508_S_AT

115
12675_S_AT
514
15372_AT
938
18512_AT

116
12678_I_AT
515
15379_AT
939
18543_AT

117
12681_S_AT
516
15381_AT
940
18544_AT

118
12688_AT
517
15383_AT
941
18552_AT

119
12702_AT
518
15384_AT
942
18555_AT

120
12705_F_AT
519
15385_AT
943
18556_AT

121
12736_F_AT
520
15387_AT
944
18561_AT

122
12737_F_AT
521
15410_AT
945
18567_AT

123
12758_AT
522
15417_S_AT
946
18573_AT

124
12760_G_AT
523
15422_AT
947
18580_AT

125
12762_R_AT
524
15423_AT
948
18581_AT

126
12764_F_AT
525
15431_AT
949
18584_AT

127
12766_AT
526
15433_AT
950
18587_S_AT

15115_F_AT
527
15452_AT
951
18588_AT

128
12767_AT
528
15464_AT
952
18591_AT

129
12768_AT
529
15468_AT
953
18592_S_AT

130
12772_AT
530
15471_AT
954
18600_AT

131
12773_AT
531
15472_AT
955
18601_S_AT

132
12776_AT
532
15475_S_AT
956
18607_S_AT

133
12788_AT
533
15485_AT
957
18611_AT

134
12793_AT
534
15489_AT
958
18616_AT

135
12794_AT
535
15490_AT
959
18622_G_AT

136
12802_AT
536
15503_AT
960
18623_AT

137
12809_G_AT
537
15505_AT
961
18628_AT

138
12812_AT
538
15510_R_AT
962
18631_AT

139
12815_AT
539
15512_AT
963
18635_AT

140
12816_AT
540
15514_AT
964
18636_AT

141
12818_AT
541
15515_R_AT
965
18638_AT

142
12824_S_AT
542
15517_S_AT
966
18652_AT

143
12828_S_AT
543
15518_AT
967
18657_AT

144
12842_S_AT
544
15529_AT
968
18659_AT

145
12846_S_AT
545
15534_F_AT
969
18660_S_AT

146
12858_AT
546
15538_AT
970
18667_AT

147
12860_S_AT
547
15541_AT
971
18675_AT

148
12861_S_AT
548
15543_AT
972
18684_AT

149
12881_S_AT
549
15544_AT
973
18686_S_AT

17600_S_AT
550
15551_AT
974
18688_S_AT

150
12889_S_AT
551
15574_S_AT
975
18693_S_AT

151
12901_S_AT
552
15576_S_AT
976
18698_S_AT

152
12902_AT
553
15577_S_AT
977
18705_AT

153
12904_S_AT
554
15578_S_AT
978
18707_AT

154
12905_S_AT
555
15583_S_AT
979
18708_AT

155
12908_S_AT
556
15588_S_AT
980
18726_S_AT

156
12910_S_AT
557
15595_S_AT
981
18727_AT

16385_S_AT
558
15600_S_AT
982
18732_I_AT

157
12914_S_AT
559
15602_F_AT
983
18736_AT

15783_S_AT
560
15608_S_AT
984
18750_F_AT

17645_S_AT
561
15613_S_AT
985
18754_AT

158
12916_S_AT
562
15616_S_AT
986
18778_AT

159
12923_S_AT
563
15618_S_AT
987
18806_S_AT

160
12926_S_AT
564
15620_S_AT
988
18823_S_AT

161
12927_S_AT
565
15627_S_AT
989
18829_AT

162
12931_S_AT
566
15634_S_AT
990
18835_AT

163
12937_R_AT

16125_S_AT
991
18844_AT

164
12941_G_AT

18046_S_AT
992
18859_AT

165
12942_AT
567
15637_S_AT
993
18864_AT

166
12947_AT
568
15639_S_AT
994
18866_AT

167
12949_AT
569
15642_S_AT
995
18880_AT

168
12953_AT
570
15643_S_AT
996
18883_G_AT

169
12956_I_AT
571
15651_F_AT
997
18885_AT

170
12959_AT
572
15652_S_AT
998
18886_AT

171
12966_S_AT
573
15665_S_AT
999
18887_AT

172
12975_AT
574
15667_S_AT
1000
18888_AT

173
12983_AT

18610_S_AT
1001
18889_AT

174
12984_AT
575
15668_S_AT
1002
18892_S_AT

175
12987_S_AT
576
15671_S_AT
1003
18901_AT

176
12994_S_AT
577
15675_S_AT
1004
18911_AT

177
13002_AT
578
15679_S_AT
1005
18917_I_AT

178
13009_I_AT
579
15685_S_AT
1006
18939_AT

179
13011_AT
580
15687_F_AT
1007
18947_I_AT

180
13018_AT
581
15688_S_AT
1008
18950_AT

181
13023_AT
582
15689_S_AT
1009
18951_S_AT

182
13024_AT
583
15692_S_AT
1010
18954_AT

183
13034_S_AT
584
15694_S_AT
1011
18956_AT

184
13046_G_AT
585
15712_S_AT
1012
18959_AT

185
13048_S_AT
586
15808_AT
1013
18966_AT

13495_S_AT
587
15845_AT
1014
18974_AT

186
13054_AT
588
15848_AT
1015
18976_AT

187
13067_S_AT
589
15850_AT
1016
18980_AT

188
13068_AT

20406_G_AT
1017
18989_S_AT

189
13073_S_AT
590
15858_AT
1018
18994_AT

190
13078_S_AT
591
15862_AT
1019
19030_AT

191
13079_AT
592
15868_AT
1020
19039_AT

192
13081_S_AT
593
15878_AT
1021
19049_AT

193
13083_AT
594
15894_AT
1022
19083_AT

194
13086_R_AT
595
15900_AT
1023
19115_AT

195
13087_AT
596
15901_AT
1024
19117_S_AT

196
13090_AT
597
15902_AT
1025
19122_AT

197
13092_S_AT
598
15912_AT
1026
19125_S_AT

16950_S_AT
599
15913_AT
1027
19127_AT

198
13098_AT
600
15928_AT
1028
19130_AT

199
13100_AT
601
15940_AT
1029
19144_AT

200
13103_AT
602
15941_AT
1030
19157_S_AT

201
13105_AT
603
15945_AT
1031
19178_AT

202
13107_S_AT
604
15948_S_AT
1032
19190_G_AT

203
13108_AT
605
15956_AT
1033
19198_AT

204
13109_AT
606
15960_AT
1034
19202_AT

205
13114_AT

16466_S_AT
1035
19209_S_AT

206
13118_F_AT
607
15976_AT
1036
19211_AT

207
13119_AT
608
15978_AT
1037
19218_AT

208
13120_AT
609
15986_S_AT
1038
19222_AT

209
13123_AT
610
15990_AT
1039
19226_G_AT

210
13128_AT
611
16009_S_AT
1040
19229_AT

211
13133_S_AT
612
16015_AT
1041
19230_AT

17430_S_AT
613
16019_AT
1042
19232_S_AT

212
13135_S_AT
614
16024_AT
1043
19285_AT

213
13139_AT
615
16034_AT
1044
19326_AT

214
13140_AT
616
16036_I_AT
1045
19332_AT

215
13143_AT

18729_AT
1046
19346_AT

216
13151_G_AT
617
16039_S_AT
1047
19347_AT

217
13160_AT
618
16040_AT
1048
19362_AT

218
13161_AT
619
16042_S_AT
1049
19363_AT

219
13162_AT
620
16047_AT
1050
19364_AT

220
13165_AT
621
16049_S_AT
1051
19367_AT

221
13166_AT
622
16051_S_AT
1052
19373_AT

222
13167_AT
623
16055_S_AT
1053
19381_AT

223
13179_AT
624
16059_S_AT
1054
19382_AT

224
13181_AT
625
16062_S_AT
1055
19384_AT

225
13185_AT
626
16066_S_AT
1056
19401_AT

226
13193_S_AT
627
16069_S_AT
1057
19406_AT

227
13213_S_AT
628
16074_S_AT
1058
19413_AT

16004_S_AT
629
16076_S_AT
1059
19416_AT

228
13219_S_AT
630
16077_S_AT
1060
19426_S_AT

20288_G_AT

17579_S_AT
1061
19439_AT

229
13220_S_AT
631
16079_S_AT
1062
19441_S_AT

13221_AT
632
16084_S_AT
1063
19442_AT

18929_S_AT

17998_S_AT
1064
19448_S_AT

230
13233_AT
633
16087_S_AT
1065
19454_AT

14301_S_AT
634
16089_S_AT
1066
19462_S_AT

231
13243_R_AT
635
16090_S_AT
1067
19464_AT

232
13254_S_AT
636
16102_S_AT
1068
19470_AT

233
13260_S_AT
637
16103_S_AT
1069
19483_AT

15660_S_AT
638
16108_S_AT
1070
19489_S_AT

234
13273_S_AT
639
16112_S_AT
1071
19513_AT

16105_S_AT
640
16134_S_AT
1072
19548_AT

235
13274_S_AT
641
16137_S_AT
1073
19562_AT

17077_S_AT
642
16138_S_AT
1074
19563_S_AT

236
13276_S_AT
643
16140_S_AT
1075
19567_AT

237
13278_F_AT
644
16143_S_AT
1076
19581_AT

238
13285_S_AT
645
16145_S_AT
1077
19589_S_AT

239
13288_S_AT
646
16148_S_AT
1078
19595_S_AT

17043_S_AT
647
16151_S_AT
1079
19606_AT

240
13292_S_AT
648
16155_S_AT
1080
19623_AT

241
13296_S_AT
649
16158_F_AT
1081
19624_AT

242
13297_S_AT
650
16160_F_AT
1082
19627_S_AT

243
13299_S_AT
651
16162_S_AT
1083
19636_AT

15166_S_AT
652
16168_S_AT
1084
19652_AT

244
13332_AT
653
16169_S_AT
1085
19655_AT

245
13347_AT
654
16171_S_AT
1086
19657_S_AT

246
13351_AT
655
16172_S_AT
1087
19658_AT

247
13352_AT
656
16184_AT
1088
19660_AT

248
13355_AT
657
16192_AT
1089
19665_S_AT

249
13404_AT
658
16222_AT
1090
19667_AT

250
13422_AT
659
16242_AT
1091
19671_AT

251
13459_AT
660
16244_AT
1092
19677_AT

252
13460_AT
661
16250_AT
1093
19686_AT

253
13461_S_AT
662
16286_AT
1094
19689_AT

254
13467_AT
663
16288_AT
1095
19690_S_AT

255
13488_AT
664
16294_S_AT
1096
19695_AT

256
13523_S_AT
665
16296_AT
1097
19698_AT

257
13529_AT
666
16297_AT
1098
19700_S_AT

258
13539_I_AT
667
16325_AT
1099
19708_AT

14631_S_AT
668
16346_S_AT
1100
19717_AT

259
13541_AT
669
16357_AT
1101
19726_S_AT

260
13542_AT
670
16380_AT
1102
19744_AT

261
13545_S_AT
671
16382_AT
1103
19752_S_AT

262
13552_AT
672
16393_S_AT
1104
19759_AT

263
13556_I_AT
673
16402_S_AT
1105
19782_AT

264
13561_AT
674
16411_S_AT
1106
19803_S_AT

265
13563_S_AT
675
16442_S_AT
1107
19828_AT

266
13567_AT
676
16446_AT
1108
19831_I_AT

267
13568_AT
677
16448_G_AT
1109
19833_S_AT

268
13571_AT
678
16453_S_AT
1110
19834_AT

269
13575_AT
679
16457_S_AT
1111
19836_AT

270
13576_AT
680
16465_AT
1112
19841_AT

271
13583_AT

16916_S_AT
1113
19845_G_AT

272
13598_AT
681
16470_S_AT
1114
19854_AT

273
13601_AT

18735_S_AT
1115
19855_AT

274
13604_AT
682
16481_S_AT
1116
19866_AT

275
13613_AT
683
16486_AT
1117
19867_AT

276
13616_S_AT
684
16487_AT
1118
19870_S_AT

16544_S_AT
685
16488_AT
1119
19871_AT

277
13617_AT
686
16496_S_AT
1120
19872_AT

278
13618_S_AT
687
16499_AT
1121
19875_S_AT

279
13619_AT
688
16510_AT
1122
19876_AT

280
13621_G_AT
689
16511_AT
1123
19879_S_AT

281
13623_R_AT
690
16512_S_AT
1124
19881_AT

282
13629_S_AT

18085_R_AT
1125
19897_S_AT

283
13631_AT
691
16514_AT
1126
19903_AT

284
13635_AT
692
16516_AT
1127
19905_AT

285
13646_AT
693
16517_AT
1128
19906_AT

286
13650_AT
694
16526_AT
1129
19907_AT

287
13653_AT
695
16528_AT
1130
19910_AT

288
13655_AT
696
16531_S_AT
1131
19913_AT

289
13656_AT
697
16535_S_AT
1132
19920_S_AT

290
13657_AT
698
16537_S_AT
1133
19932_AT

291
13666_S_AT
699
16538_S_AT
1134
19939_AT

17083_S_AT
700
16543_S_AT
1135
19945_AT

292
13667_S_AT
701
16550_S_AT
1136
19947_AT

293
13669_S_AT
702
16554_S_AT
1137
19951_AT

17074_S_AT
703
16567_S_AT
1138
19956_AT

294
13670_S_AT
704
16571_S_AT
1139
19962_AT

15206_S_AT
705
16576_F_AT
1140
19963_AT

295
13671_S_AT
706
16577_S_AT
1141
19969_AT

16805_S_AT
707
16579_S_AT
1142
19970_S_AT

296
13678_S_AT
708
16580_S_AT
1143
19971_AT

297
13688_S_AT
709
16583_S_AT
1144
19972_AT

298
13690_S_AT
710
16584_S_AT
1145
19981_AT

16065_S_AT

18706_S_AT
1146
19990_AT

299
13691_S_AT
711
16593_S_AT
1147
19996_AT

16117_S_AT
712
16595_S_AT
1148
20003_S_AT

300
13692_S_AT
713
16598_S_AT
1149
20009_S_AT

16118_S_AT
714
16604_S_AT
1150
20013_AT

301
13700_AT
715
16605_S_AT
1151
20018_AT

302
13704_S_AT
716
16610_S_AT
1152
20024_S_AT

303
13714_AT
717
16611_S_AT
1153
20027_AT

304
13715_AT
718
16614_S_AT
1154
20045_AT

305
13724_AT
719
16617_S_AT
1155
20047_AT

306
13748_AT
720
16618_S_AT
1156
20048_AT

307
13759_AT
721
16620_S_AT
1157
20050_AT

308
13767_AT
722
16621_S_AT
1158
20051_AT

309
13785_AT
723
16631_S_AT
1159
20058_AT

310
13803_AT
724
16634_S_AT
1160
20067_AT

311
13850_I_AT
725
16635_S_AT
1161
20068_AT

312
13876_AT
726
16636_S_AT
1162
20069_AT

313
13880_S_AT
727
16639_S_AT
1163
20093_I_AT

314
13883_AT
728
16640_S_AT
1164
20099_AT

315
13887_S_AT
729
16650_S_AT
1165
20100_AT

316
13895_AT
730
16652_S_AT
1166
20113_S_AT

317
13904_S_AT
731
16654_AT
1167
20117_AT

18722_S_AT
732
16672_AT
1168
20123_AT

318
13906_S_AT
733
16673_AT
1169
20127_S_AT

319
13908_S_AT
734
16687_S_AT
1170
20129_AT

18597_AT
735
16747_AT
1171
20150_AT

320
13923_AT
736
16753_AT
1172
20154_AT

321
13927_AT
737
16768_AT
1173
20156_AT

322
13932_AT
738
16777_AT
1174
20165_AT

323
13935_AT
739
16784_AT
1175
20173_AT

324
13940_AT
740
16807_AT
1176
20178_S_AT

325
13949_S_AT
741
16811_AT
1177
20183_AT

326
13954_G_AT
742
16845_AT
1178
20188_AT

327
13971_S_AT
743
16894_AT
1179
20189_AT

328
13973_AT
744
16899_AT
1180
20197_AT

329
13983_AT
745
16911_AT
1181
20210_G_AT

330
13985_S_AT
746
16920_AT
1182
20213_AT

331
13987_S_AT
747
16921_AT
1183
20229_AT

18738_F_AT
748
16924_S_AT
1184
20232_S_AT

332
13989_AT
749
16926_S_AT
1185
20255_AT

20674_S_AT
750
16931_S_AT
1186
20257_AT

333
14010_AT
751
16934_S_AT
1187
20262_AT

334
14013_AT
752
16937_AT
1188
20275_AT

335
14014_AT
753
16938_AT
1189
20278_S_AT

336
14019_AT
754
16942_AT
1190
20282_S_AT

337
14021_R_AT
755
16943_S_AT
1191
20284_AT

338
14025_S_AT

18231_AT
1192
20293_AT

18909_S_AT
756
16949_S_AT
1193
20294_AT

339
14027_AT
757
16952_S_AT
1194
20312_S_AT

340
14030_AT
758
16956_AT
1195
20315_I_AT

341
14044_AT
759
16962_S_AT
1196
20330_S_AT

342
14048_AT
760
16965_S_AT
1197
20331_AT

343
14056_AT
761
16970_S_AT
1198
20350_S_AT

344
14057_AT

18010_S_AT
1199
20354_S_AT

345
14058_AT
762
16977_AT
1200
20355_AT

346
14059_AT
763
16984_AT
1201
20360_AT

347
14061_AT
764
16996_S_AT
1202
20363_AT

348
14068_S_AT
765
16997_AT
1203
20369_S_AT

349
14072_AT
766
17000_AT
1204
20378_G_AT

350
14073_AT
767
17005_AT
1205
20383_AT

351
14074_AT
768
17010_S_AT
1206
20384_AT

352
14084_AT
769
17017_S_AT
1207
20387_AT

353
14095_S_AT
770
17031_S_AT
1208
20393_AT

354
14100_AT
771
17033_S_AT
1209
20396_AT

355
14101_AT
772
17053_S_AT
1210
20399_AT

356
14103_AT
773
17055_S_AT
1211
20409_G_AT

357
14105_AT
774
17063_S_AT
1212
20412_S_AT

358
14106_AT
775
17068_S_AT
1213
20413_AT

359
14121_AT
776
17070_S_AT
1214
20439_AT

360
14129_S_AT
777
17075_S_AT
1215
20440_AT

361
14133_S_AT
778
17084_S_AT
1216
20444_AT

362
14143_AT
779
17087_S_AT
1217
20445_AT

363
14145_AT
780
17092_S_AT
1218
20449_AT

364
14148_AT
781
17095_S_AT
1219
20456_AT

365
14186_AT
782
17096_S_AT
1220
20462_AT

366
14194_AT
783
17102_S_AT
1221
20471_AT

367
14196_AT
784
17105_S_AT
1222
20474_AT

368
14223_AT
785
17109_S_AT
1223
20495_S_AT

369
14234_AT
786
17110_S_AT
1224
20499_AT

370
14236_AT
787
17113_S_AT
1225
20501_AT

371
14251_F_AT
788
17115_S_AT
1226
20511_AT

372
14252_F_AT
789
17116_S_AT
1227
20515_S_AT

373
14270_AT
790
17123_S_AT
1228
20516_AT

374
14298_G_AT
791
17129_S_AT
1229
20517_AT

17581_G_AT
792
17132_AT
1230
20518_AT

375
14303_S_AT
793
17166_AT
1231
20520_S_AT

376
14312_AT
794
17206_AT
1232
20536_S_AT

377
14316_AT
795
17207_AT
1233
20538_S_AT

378
14339_AT
796
17215_AT
1234
20539_S_AT

379
14366_AT
797
17237_AT
1235
20558_AT

380
14369_AT
798
17247_AT
1236
20561_AT

381
14388_AT
799
17254_AT
1237
20567_AT

382
14392_G_AT
800
17286_AT
1238
20571_AT

383
14393_AT
801
17288_S_AT
1239
20582_S_AT

384
14421_AT
802
17292_AT
1240
20586_I_AT

385
14436_AT
803
17300_AT
1241
20590_AT

386
14448_AT
804
17303_S_AT
1242
20592_AT

387
14450_AT
805
17318_AT
1243
20594_AT

388
14454_AT
806
17319_AT
1244
20608_S_AT

389
14459_AT
807
17322_AT
1245
20612_S_AT

390
14478_AT
808
17323_AT
1246
20616_AT

391
14482_AT
809
17332_S_AT
1247
20620_G_AT

392
14485_AT
810
17374_AT
1248
20637_AT

393
14492_S_AT
811
17381_AT
1249
20643_AT

394
14505_AT
812
17388_AT
1250
20649_AT

395
14510_AT
813
17392_S_AT
1251
20651_AT

396
14511_AT
814
17405_AT
1252
20654_S_AT

397
14517_AT
815
17415_AT
1253
20670_AT

398
14519_AT
816
17418_S_AT
1254
20684_AT

399
14525_S_AT
817
17420_AT
1255
20685_AT

400
14527_AT
818
17423_S_AT
1256
20693_AT

401
14534_S_AT
819
17426_AT
1257
20701_S_AT

402
14538_R_AT
820
17427_AT
1258
20704_AT

403
14554_AT
821
17429_S_AT
1259
20705_AT

404
14558_AT
822
17431_AT
1260
20715_AT

405
14559_S_AT
823
17439_G_AT
1261
20719_AT

[0207]

4

TABLE 4

2X UP IN COLD, ONLY

11997_at
14965_at
17675_at

11998_at
14984_s _at
17677_at

12018_at
15004_at
17693_at

12031_at
15010_at
17732_at

12047_at
15036_r_at
17743_at

12051_at
15040_g_at
17748_at

12053_at
15046_s_at
17775_at

12060_at
15057_at
17782_at

12072_at
15073_at
17841_at

12074_at
15083_at
17852_g_at

12102_at
15084_at
17900_s_at

12112_at
15096_at
17901_at

12117_at
15101_s_at
17911_at

12130_at
15105_s_at
17921_s_at

12145_s_at
15112_s_at
17922_at

12151_at
15115_f_at
17933_at

12163_at
15116_f_at
17967_at

12175_at
15122_s_at
17970_i_at

12187_at
15126_s_at
17978_s_at

12195_at
15131_s_at
17999_at

12219_at
15132_s_at
18001_at

12256_at
15137_s_at
18004_at

12269_s_at
15144_s_at
18012_s_at

12307_at
15148_s_at
18040_s_at

12315_at
15153_s_at
18176_at

12336_at
15159_s_at
18194_i_at

12349_s_at
15160_s_at
18197_at

12353_at
15166_s_at
18198_at

12359_s_at
15174_f_at
18213_at

12390_at
15197_s_at
18219_at

12395_s_at
15270_at
18222_at

12431_at
15319_at
18231_at

12436_at
15325_at
18232_at

12443_s_at
15337_at
18241_at

12447_at
15341_at
18269_s_at

12452_at
15343_at
18272_at

12477_at
15355_s_at
18282_at

12503_at
15367_at
18298_at

12516_s_at
15379_at
18316_at

12532_at
15381_at
18317_at

12544_at
15410_at
18331_s_at

12561_at
15417_s_at
18347_s_at

12602_at
15422_at
18383_at

12610_at
15433_at
18390_at

12631_at
15451_at
18455_at

12647_s_at
15452_at
18465_s_at

12650_at
15453_s_at
18544_at

12656_at
15472_at
18555_at

12674_at
15489_at
18556_at

12675_s_at
15490_at
18560_at

12676_s_at
15503_at
18561_at

12681_s_at
15510_r_at
18571_at

12686_s_at
15517_s_at
18588_at

12688_at
15518_at
18597_at

12701_i_at
15544_at
18601_s_at

12702_at
15588_s_at
18611_at

12719_f_at
15600_s_at
18623_at

12726_f_at
15605_s_at
18635_at

12736_f_at
15613_s_at
18659_at

12754_g_at
15614_s_at
18660_s_at

12762_r_at
15616_s_at
18673_at

12766_at
15633_s_at
18694_s_at

12767_at
15639_s_at
18705_at

12768_at
15641_s_at
18708_at

12773_at
15660_s_at
18738_f_at

12788_at
15665_s_at
18750_f_at

12802_at
15687_f_at
18778_at

12860_s_at
15694_s_at
18829_at

12861_s_at
15712_s_at
18835_at

12879_s_at
15783_s_at
18866_at

12891_at
15808_at
18875_s_at

12914_s_at
15837_at
18885_at

12927_s_at
15850_at
18887_at

12947_at
15862_at
18888_at

12956_i_at
15868_at
18889_at

12966_s_at
15878_at
18901_at

12974_at
15901_at
18907_s_at

12987_s_at
15912_at
18917_i_at

12994_s_at
15920_i_at
18939_at

12998_at
15941_at
18947_i_at

13002_at
15945_at
18949_at

13018_at
15960_at
18954_at

13023_at
15990_at
18959_at

13046_g_at
16001_at
18974_at

13054_at
16009_at
18976_at

13086_r_at
16010_s_at
18980_at

13087_at
16034_at
18989_s_at

13100_at
16036_i_at
19019_i_at

13109_at
16039_s_at
19049_at

13119_at
16040_at
19083_at

13120_at
16042_s_at
19130_at

13128_at
16047_at
19156_s_at

13134_s_at
16049_s_at
19178_at

13140_at
16051_s_at
19190_g_at

13143_at
16062_s_at
19199_at

13167_at
16079_s_at
19202_at

13172_s_at
16087_s_at
19209_s_at

13178_at
16090_s_at
19211_at

13179_at
16117_s_at
19218_at

13181_at
16118_s_at
19229_at

13187_i_at
16137_s_at
19322_at

13209_s_at
16155_s_at
19326_at

13219_s_at
16162_s_at
19359_s_at

13221_at
16184_at
19367_at

13243_r_at
16192_at
19384_at

13260_s_at
16222_at
19389_at

13274_a_at
16244_at
19397_at

13278_f_at
16250_at
19406_at

13279_s_at
16260_at
19426_s_at

13285_s_at
16286_at
19441_s_at

13288_s_at
16296_at
19442_at

13292_s_at
16297_at
19470_at

13297_s_at
16342_at
19489_s_at

13299_s_at
16367_i_at
19562_at

13332_at
16411_s_at
19577_at

13351_at
16442_s_at
19589_s_at

13352_at
16465_at
19597_s_at

13422_at
16466_s_at
19611_s_at

13435_at
16468_at
19624_at

13461_s_at
16486_at
19657_s_at

13467_at
16487_at
19667_at

13488_at
16488_at
19671_at

13495_s_at
16489_at
19677_at

13539_i_at
16496_s_at
19686_at

13542_at
16499_at
19689_at

13575_at
16511_at
19698_at

13577_s_at
16517_at
19700_s_at

13617_at
16538_s_at
19707_s_at

13634_s_at
16554_s_at
19708_AT

13656_at
16571_s_at
19713_at

13671_s_at
16576_f_at
19718_at

13691_s_at
16595_s_sat
19744_at

13700_at
16605_s_at
19836_at

13704_s_at
16610_s_at
19839_at

13709_s_at
16620_s_at
19840_s_at

13715_at
16621_s_at
19845_g_at

13785_at
16635_s_at
19854_at

13803_at
16636_s_at
19855_at

13812_s_at
16638_s_at
19860_at

13825_s_at
16650_s_at
19866_at

13850_i_at
16672_at
19871_at

13904_s_at
16673_at
19875_s_at

13908_s_at
16687_s_at
19879_s_at

13927_at
16747_at
19881_at

13971_s_at
16753_at
19913_at

13985_s_at
16768_at
19939_at

14013_at
16805_s_at
19945_at

14019_at
16807_at
19947_at

14021_r_at
16845_at
19951_at

14028_at
16847_at
19956_at

14048_at
16896_s_at
19971_at

14058_at
16899_at
19976_at

14059_at
16902_at
19998_at

14064_at
16911_at
20003_s_at

14073_at
16914_s_at
20015_at

14105_at
16943_s_at
20027_at

14106_at
16956_at
20051_at

14126_s_at
16996_s_at
20068_at

14140_at
17010_s_at
20093_i_at

14145_at
17016_s_at
20117_at

14170_at
17032_s_at
20150_at

14186_at
17033_s_at
20156_at

14196_at
17043_s_at
20165_at

14227_at
17050_s_at
20257_at

14234_at
17055_s_at
20262_at

14250_r_at
17068_s_at
20275_at

14270_at
17071_s_at
20282_s_at

14298_g_at
17075_s_at
20288_g_at

14303_s_at
17077_s_at
20293_at

14312_at
17102_s_at
20315_i_at

14339_at
17109_s_at
20330_s_at

14388_at
17113_s_at
20360_at

14393_at
17123_s_at
20363_at

14511_at
17128_s_at
20369_s_at

14525_s_at
17129_s_at
20384_at

14527_at
17132_at
20393_at

14534_s_at
17166_at
20396_at

14554_at
17206_at
20412_s_at

14566_at
17237_at
20413_at

14579_at
17300_at
20432_at

14591_at
17319_at
20433_at

14595_at
17322_at
20456_at

14600_at
17322_s_at
20462_at

14631_s_at
17381_at
20471_at

14635_s_at
17388_at
20511_at

14679_s_at
17392_s_at
20515_s_at

14691_at
17408_at
20517_at

14697_g_at
17424_at
20518_at

14709_at
17429_s_at
20529_at

14711_s_at
17457_at
20536_s_at

14728_s_at
17458_at
20538_s_at

14731_s_at
17466_s_at
20539_s_at

14797_s_at
17477_s_at
20576_at

14809_at
17482_s_at
20582_s_at

14843_at
17538_s_at
20586_i_at

14847_at
17546_s_at
20608_s_at

14872_at
17562_at
20649_at

14886_at
17581_g_at
20651_at

14896_at
17627_at
20684_at

14897_at
17631_at
20685_at

14900_at
17632_at
20699_at

14956_s_at
17645_s_at
20705_at

14958_at
17672_at
20715_at

[0208]

5

TABLE 5

2X UP COLD 3 HR, ONLY

12117_at
13671_s_at
15453_s_at
17237_at
19624_at

12145_s_at
13691_s_at
15489_at
17319_at
19657_s_t

12151_at
13785_at
15518_at
17392_s_at
19667_at

12163_at
13803_at
15588_s_at
17429_s_at
19845_g_at

12187_at
13825_s_at
15613_s_at
17477_s_at
19855_at

12256_at
13904_s_at
15614_s_at
17538_s_at
19866_at

12315_at
14013_at
15616_s_at
17581_g_at
19945_at

12349_s_at
14021_r_at
15639_s_at
17627_at
19951_at

12353_at
14028_at
15641_s_at
17672_at
19998_at

12359_s_at
14064_at
15660_s_at
17693_at
20003_s_at

12544_at
14126_s_at
15687_f_at
17782_at
20015_at

12602_at
14145_at
15694_s_at
17841_at
20051_at

12610_at
14170_at
15862_at
17900_s_at
20093_i_at

12676_s_at
14196_at
15868_at
17933_at
20117_at

12686_s_at
14250_r_at
15878_at
17978_s_at
20288_g_at

12701_i_at
14298_g_at
15901_at
18001_at
20360_at

12702_at
14303_s_at
16034_at
18012_s_at
20369_s_at

12719_f_at
14339_at
16039_s_at
18198_at
20384_at

12736_f_at
14527_at
16040_at
18219_at
20462_at

12754_g_at
14534_s_at
16042_s_at
18241_at
20471_at

12766_at
14554_at
16047_at
18269_s_at
20515_s_at

12767_at
14595_at
16062_s_at
18272_at
20538_s_at

12768_at
14635_s_at
16087_s_at
18282_at
20576_at

12773_at
14679_s_at
16117_s_at
18298_at
20608_s_at

12788_at
14691_at
16118_s_at
18383_at
20651_at

12879_s_at
14697_g_at
16162_s_at
18556_at
20685_at

12891_at
14709_at
16184_at
18588_at
20705_at

12947_at
14728_s_at
16222_at
18601_s_at

12966_s_at
14809_at
16250_at
18611_at

12974_at
14896_at
16411_s_at
18694_s_at

12994_s_at
14965_at
16442_s_at
18708_at

13002_at
14984_s_at
16465_at
18738_f_at

13100_at
15046_s_at
16486_at
18778_at

13140_at
15083_at
16488_at
18829_at

13167_at
15096_at
16489_at
18835_at

13172_s_at
15105_s_at
16517_at
18866_at

13179_at
15115_f_at
16571_s_at
18875_s_at

13187_i_at
15116_f_at
16605_s_at
18888_at

13219_s_at
15122_s_at
16610_s_at
18907_s_at

13260_s_at
15126_s_at
16620_s_at
18917_i_at

13278_f_at
15131_s_at
16636_s_at
18939_at

13279_s_at
15132_s_at
16650_s_at
18974_at

13285_s_at
15137_s_at
16805_s_at
19190_g_at

13288_s_at
15153_s_at
16845_at
19199_at

13292_s_at
15159_s_at
16899_at
19202_at

13297_s_at
15160_s_at
16914_s_at
19211_at

13351_at
15197_s_at
16943_s_at
19384_at

13352_at
15355_s_at
16996_s_at
19406_at

13435_at
15379_at
17010_s_at
19426_s_at

13467_at
15417_s_at
17043_s_at
19442_at

13488_at
15422_at
17068_s_at
19470_at

13495_s_at
15451_at
17109_s_at
19577_at

13656_at
15452_at
17128_s_at
19597_s_at

[0209]

6

TABLE 6

2X DOWN COLD, ONLY

11991_g_at
14672_s_at
17578_at

11992_at
14673_s_at
17579_s_at

12001_at
14675_s_at
17585_s_at

12006_s_at
14679_s_at
17596_at

12007_at
14681_g_at
17600_s_at

12009_at
14682_i_at
17823_s_at

12022_at
14689_at
17840_s_at

12023_s_at
14701_s_at
17849_s_at

12026_at
14703_at
17857_at

12037_at
14712_s_at
17865_at

12052_at
14713_s_at
17882_at

12125_at
14715_s_at
17885_at

12143_at
14734_s_at
17902_s_at

12149_at
14781_at
17910_at

12156_at
14800_at
17916_at

12166_i_at
14856_s_at
17917_s_at

12167_at
14882_at
17918_at

12169_at
14908_at
17926_s_at

12176_at
14912_at
17935_at

12179_at
14914_at
17956_i_at

12196_at
14924_at
17961_at

12198_at
14942_at
17966_at

12200_at
14945_at
17978_s_at

12202—‘at
14955_at
17986_s_at

12212_at
14957_s_at
17993_at

12214_g_at
14974_at
17998_s_at

12224_at
14980_at
18003_at

12226_at
14981_at
18005_at

12233_at
14995_at
18010_s_at

12240_at
15009_at
18013_r_at

12253_g_at
15018_at
18023_s_at

12270_at
15024_at
18029_g_at

12278_at
15026_at
18030_i_at

12284_at
15047_at
18045_at

12287_s_at
15054_at
18046_s_at

12293_at
15056_at
18059_i_at

12294_s_at
15058_s_at
18064_r_at

12300_at
15063_at
18065_r_at

12312_at
15066_at
18074_at

12315_at
15081_at
18076_s_at

12324_i_at
15091_at
18077_at

12331_s_at
15097_s_at
18078_at

12344_at
15102_s_at
18081_at

12348_at
15107_s_at
18083_r_at

12353_at
15118_s_at
18085_r_at

12372_at
15127_s_at
18091_at

12374_i_at
15130_s_at
18154_s_at

12405_at
15132_s_at
18165_at

12408_at
15133_s_at
18174_at

12410_g_at
15139_s_at
18221_at

12419_at
15143_s_at
18226_s_at

12427_at
15146_s_at
18230_at

12438_at
15150_s_at
18237_at

12450_s_at
15161_s_at
18255_at

12474_at
15162_s_at
18257_at

12491_at
15167_s_at
18258_s_at

12497_at
15170_s_at
18274_s_at

12500_s_at
15171_s_at
18275_at

12515_at
15178_s_at
18278_at

12521_at
15182_s_at
18283_at

12523_at
15185_s_at
18290_at

12526_at
15188_s_at
18291_at

12527_at
15193_s_at
18299_s_at

12534_g_at
15196_s_at
18300_at

12549_s_at
15201_f_at
18306_at

12550_s_at
15206_s_at
18327_s_at

12552_at
15207_s_at
18337_s_at

12555_s_at
15213_s_at
18339_at

12556_at
15243_at
18365_s_at

12575_s_at
15256_at
18402_at

12576_s_at
15348_at
18439_s_at

12581_s_at
15350_at
18487_at

12587_at
15372_at
18508_s_at

12597_at
15383_at
18512_at

12606_at
15384_at
18543_at

12609_at
15385_at
18552_at

12646_at
15387_at
18567_at

12649_at
15406_at
18573_at

12653_at
15423_at
18580_at

12661_at
15431_at
18581_at

12666_at
15464_at
18584_at

12678_i_at
15468_at
18587_s_at

12705_f_at
15471_at
18590_at

12736_f_at
15475_s_at
18591_at

12737_f_at
15485_at
18592_s_at

12758_at
15505_at
18600_at

12760_g_at
15512_at
18601_s_at

12764_f_at
15514_at
18607_s_at

12765_at
15515_r_at
18610_s_at

12772_at
15529_at
18611_at

12776_at
15534_f_at
18616_at

12784_at
15538_at
18622_g_at

12793_at
15541_at
18628_at

12794_at
15543_at
18631_at

12795_at
15551_at
18636_at

12809_g_at
15574_s_at
18638_at

12812_at
15576_s_at
18652_at

12815_at
15577_s_at
18657_at

12816_at
15578_s_at
18667_at

12818_at
15581_s_at
18675_at

12824_s_at
15583_s_at
18684_at

12828_s_at
15591_s_at
18686_s_at

12842_s_at
15595_s_at
18688_s_at

12846_s_at
15602_f_at
18693_s_at

12858_at
15606_s_at
18698_s_at

12869_s_at
15608_s_at
18706_s_at

12881_s_at
15616_s_at
18707_at

12889_s_at
15618_s_at
18726_s_at

12901_s_at
15620_s_at
18727_at

12902_at
15627_s_at
18732_i_at

12904_s_at
15634_s_at
18735_s_at

12905_s_at
15637_s_at
18736_at

12908_s_at
15642_s_at
18738_f_at

12910_s_at
15643_s_at
18747_f_at

12916_s_at
15646_s_at
18754_at

12923_s_at
15651_f_at
18782_at

12926_s_at
15652_s_at
18789_at

12931_s_at
15667_s_at
18806_s_at

12937_r_at
15668_s_at
18814_at

12941_g_at
15670_s_at
18823_s_at

12942_at
15671_s_at
18844_at

12949_at
15675_s_at
18859_at

12953_at
15679_s_at
18864_at

12958_at
15685_s_at
18880_at

12959_at
15688_s_at
18883_g_at

12966_s_at
15689_s_at
18886_at

12975_at
15692_s_at
18892_s_at

12983_at
15775_at
18909_s_at

12984_at
15776_at
18911_at

13002_at
15845_at
18913_s_at

13009_i_at
15848_at
18916_s_at

13011_at
15858_at
18921_g_at

13014_at
15866_s_at
18950_at

13024_at
15894_at
18951_s_at

13034_s_at
15900_at
18956_at

13041_s_at
15901_at
18966_at

13048_s_at
15902_at
18972_at

13067_s_at
15913_at
18994_at

13068_at
15928_at
19030_at

13073_s_at
15940_at
19039_at

13078_s_at
15948_s_at
19068_i_at

13079_at
15956_at
19108_at

13081_s_at
15976_at
19115_at

13083_at
15978_at
19117_s_at

13090_at
15986_s_at
19122_at

13092_s_at
16004_s_at
19125_s_at

13098_at
16015_at
19127_at

13103_at
16017_at
19135_at

13105_at
16019_at
19144_at

13107_s_at
16024_at
19157_s_at

13108_at
16031_at
19158_at

13114_at
16055_s_at
19177_at

13118_f_at
16059_s_at
19192_at

13123_at
16065_s_at
19198_at

13124_at
16066_s_at
19222_at

13133_s_at
16069_s_at
19226_g_at

13145_at
16074_s_at
19227_at

13139_at
16076_s_at
19230_at

13146_s_at
16077_s_at
19232_s_at

13151_g_at
16084_s_at
19263_at

13160_at
16089_s_at
19285_at

13161_at
16102_s_at
19332_at

13162_at
16103_s_at
19346_at

13165_at
16105_s_at
19347_at

13166_at
16108_s_at
19361_s_at

13185_at
16112_s_at
19362_at

13193_s_at
16117_s_at
19363_at

13211_s_at
16118_s_at
19364_at

13213_s_at
16125_s_at
19365_s_at

13219_s_at
16127_s_at
19373_at

13233_at
16134_s_at
19379_at

13236_s_at
16136_s_at
19381_at

13239_s_at
16138_s_at
19382_at

13241_s_at
16140_s_at
19401_at

13254_s_at
16143_s_at
19402_at

13266_s_at
16144_s_at
19406_at

13273_s_at
16145_s_at
19413_at

13275_f_at
16148_s_at
19416_at

13276_s_at
16151_s_at
19429_at

13278_f_at
16158_f_at
19432_s_at

13280_s_at
16160_f_at
19439_at

13285_s_at
16168_s_at
19448_s_at

13296_s_at
16169_s_at
19454_at

13347_at
16171_s_at
19462_s_at

13355_at
16172_s_at
19464_at

13361_at
16222_at
19469_at

13404_at
16232_s_at
19483_at

13406_at
16242_at
19484_s_at

13459_at
16288_at
19513_at

13460_at
16294_s_at
19548_at

13464_at
16325_at
19563_s_at

13523_s_at
16346_s_at
19567_at

13529_at
16357_at
19581_at

13541_at
16380_at
19595_s_at

13545_s_at
16382_at
19606_at

13550_at
16385_s_at
19623_at

13552_at
16393_s_at
19627_s_at

13556_i_at
16402_s_at
19636_at

13561_at
16417_s_at
19641_at

13563_s_at
16442_s_at
19652_at

13567_at
16446_at
19655_at

13568_at
16448_g_at
19658_at

13571_at
16453_s_at
19660_at

13576_at
16457_s_at
19665_s_at

13583_at
16470_s_at
19667_at

13598_at
16481_s_at
19690_s_at

13601_at
16510_at
19695_at

13604_at
16512_s_at
19717_at

13613_at
16514_at
19726_s_at

13616_s_at
16516_at
19752_s_at

13618_s_at
16523_s_at
19759_at

13619_at
16526_at
19782_at

13621_g_at
16528_at
19789_at

13623_r_at
16531_s_at
19803_s_at

13629_s_at
16535_s_at
19828_at

13631_at
16537_s_at
19831_i_at

13635_at
16543_s_at
19833_s_at

13646_at
16544_s_at
19834_at

13650_at
16550_s_at
19835_at

13652_at
16559_s_at
19841_at

13653_at
16567_s_at
19867_at

13655_at
16577_s_at
19870_s_at

13657_at
16579_s_at
19871_at

13666_at
16580_s_at
19872_at

13667_s_at
16583_s_at
19876_at

13669_s_at
16584_s_at
19897_s_at

13670_s_at
16593_s_at
19903_at

13672_s_at
16598_s_at
19905_at

13678_s_at
16603_s_at
19906_at

13679_s_at
16604_s_at
19907_at

13688_s_at
16611_s_at
19910_at

13690_s_at
16614_s_at
19920_s_at

13691_s_at
16617_s_at
19932_at

13692_s_at
16618_s_at
19951_at

13714_at
16620_s_at
19962_at

13724_at
16631_s_at
19963_at

13748_at
16634_s_at
19969_at

13751_at
16639_s_at
19970_s_at

13759_at
16640_s_at
19972_at

13767_at
16652_s_at
19981_at

13789_at
16654_at
19990_at

13876_at
16777_at
19996_at

13880_s_at
16784_at
19999_s_at

13883_at
16811_at
20009_s_at

13887_s_at
16893_at
20013_at

13895_at
16894_at
20017_at

13906_s_at
16899_at
20018_at

13919_at
16920_at
20024_s_at

13923_at
16921_at
20045_at

13932_at
16924_s_at
20047_at

13935_at
16926_s_at
20048_at

13940_at
16931_s_at
20050_at

13949_s_at
16934_s_at
20051_at

13954_g_at
16937_at
20058_at

13973_at
16938_at
20067_at

13983_at
16942_at
20069_at

13989_at
16949_s_at
20099_at

14010_at
16950_s_at
20100_at

14014_at
16952_s_at
20113_s_at

14015_s_at
16962_s_at
20123_at

14016_s_at
16965_s_at
20127_s_at

14025_s_at
16970_s_at
20129_at

14027_at
16977_at
20133_i_at

14030_at
16984_at
20152_at

14044_at
16989_at
20154_at

14056_at
16993_at
20173_at

14057_at
16997_at
20178_s_at

14061_at
17000_at
20183_at

14067_at
17005_at
20188_at

14068_s_at
17010_s_at
20189_at

14072_at
17017_s_at
20197_at

14074_at
17031_s_at
20200_at

14075_at
17040_s_at
20210_g_at

14083_at
17053_s_at
20213_at

14084_at
17056_s_at
20229_at

14089_at
17063_s_at
20232_s_at

14095_s_at
17070_s_at
20255_at

14096_at
17074_s_at
20278_s_at

14100_at
17084_s_at
20284_at

14101_at
17085_s_at
20288_g_at

14103_at
17087_s_at
20294_at

14121_at
17092_s_at
20312_s_at

14129_s_at
17095_s_at
20331_at

14133_s_at
17096_s_at
20335_s_at

14143_at
17097_s_at
20350_s_at

14148_at
17103_s_at
20354_s_at

14162_at
17105_s_at
20355_at

14194_at
17110_s_at
20369_s_at

14208_at
17115_s_at
20378_g_at

14217_at
17116_s_at
20383_at

14223_at
17119_s_at
20385_s_at

14235_at
17122_s_at
20387_at

14236_at
17207_at
20399_at

14251_f_at
17215_at
20409_g_at

14252_f_at
17247_at
20420_at

14285_at
17254_at
20429_s_at

14301_at
17286_at
20439_at

14316_at
17288_s_at
20440_at

14366_at
17292_at
20444_at

14369_at
17303_s_at
20445_at

14392_g_at
17305_at
20449_at

14421_at
17318_at
20474_at

14431_at
17323_at
20480_s_at

14436_at
17374_at
20495_s_at

14448_at
17405_at
20499_at

14450_at
17415_at
20501_at

14454_at
17418_s_at
20516_at

14459_at
17420_at
20520_s_at

14478_at
17423_s_at
20530_s_at

14482_at
17426_at
20538_s_at

14485_at
17427_at
20547_at

14492_s_at
17430_s_at
20558_at

14505_at
17431_at
20561_at

14510_at
17439_g_at
20567_at

14517_at
17442_i_at
20571_at

14519_at
17449_s_at
20590_at

14534_s_at
17462_s_at
20592_at

14538_r_at
17463_at
20594_at

14558_at
17465_at
20608_s_at

14559_s_at
17475_at
20612_s_at

14572_at
17479_at
20616_at

14584_at
17495_s_at
20620_g_at

14587_at
17508_s_at
20635_s_at

14595_at
17522_s_at
20637_at

14602_at
17523_s_at
20643_at

14603_at
17529_s_at
20654_s_at

14605_at
17537_s_at
20670_at

14620_s_at
17539_s_at
20674_s_at

14626_s_at
17543_s_at
20684_at

14630_s_at
17555_s_at
20685_at

14637_s_at
17557_s_at
20689_s_at

14640_s_at
17560_s_at
20693_at

14642_f_at
17564_s_at
20701_s_at

14650_s_at
17565_s_at
20704_at

14654_s_at
17568_s_at
20707_s_at

14667_s_at
17570_g_at
20719_at

14668_s_at
17573_at

14669_s_at
17577_g_at

[0210]

7

TABLE 7

SALINE STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX
SEQ
AFFYMETRIX

ID NO:
ID NO:
ID NO:
ID NO:

2227
12011_S_AT
2327
15995_S_AT

2228
12153_AT
2328
15998_S_AT

2229
12180_AT

18090_S_AT

2230
12186_AT
2329
16028_AT

2231
12216_AT
2330
16050_AT

2232
12265_AT
2331
16060_S_AT

2233
12335_AT
2332
16067_S_AT

2234
12449_S_AT
2333
16072_S_AT

2235
12470_AT
2334
16088_F_AT

2236
12479_AT
2335
16273_AT

2237
12487_AT
2336
16314_AT

2238
12493_G_AT
2337
16413_S_AT

2239
12562_AT
2338
16414_AT

2240
12685_AT
2339
16426_AT

2241
12704_F_AT
2340
16436_AT

2242
12709_F_AT
2341
16455_AT

2243
12734_F_AT
2342
16502_AT

2244
12739_S_AT
2343
16548_S_AT

2245
12750_S_AT
2344
16568_S_AT

2246
12761_S_AT
2345
16582_S_AT

2247
12813_AT
2346
16589_S_AT

2248
12845_S_AT
2347
16594_S_AT

2249
12946_AT
2348
16613_S_AT

2250
13003_S_AT
2349
16651_S_AT

2511
3052_S_AT
2350
16668_AT

2252
13094_AT
2351
16820_AT

2253
13142_AT
2352
16987_S_AT

2254
13172_S_AT
2353
16995_AT

17880_S_AT
2354
17039_S_AT

2255
13198_I_AT
2355
17273_AT

2256
13209_S_AT
2356
17278_AT

16165_S_AT
2357
17433_AT

2257
13229_S_AT
2358
17467_AT

2258
13253_F_AT
2359
17566_AT

2259
13344_S_AT
2360
17595_S_AT

2260
13370_AT
2361
17744_S_AT

2261
13387_AT
2362
17758_AT

2262
13408_S_AT
2363
17864_AT

2263
13429_AT
2364
17868_AT

2264
13472_AT
2365
17876_AT

2265
13526_AT
2366
17894_AT

2266
13569_AT
2367
17942_S_AT

2267
13614_AT
2368
18008_R_AT

2268
13686_S_AT
2369
18027_AT

2269
13718_AT
2370
18053_S_AT

2270
13719_AT
2371
18062_AT

2271
13902_AT
2372
18082_AT

2272
13918_AT
2373
18121_S_AT

2273
13944_AT
2374
18240_S_AT

2274
13964_AT
2375
18248_S_AT

2275
13993_S_AT
2376
18264_AT

2276
14000_AT
2377
18276_AT

2277
14003_AT
2378
18287_AT

2278
14032_AT
2379
18310_AT

2279
14043_AT
2380
18367_S_AT

2280
14070_AT
2381
18506_AT

2281
14267_AT
2382
18605_S_AT

2282
14269_AT
2383
18618_S_AT

2283
14418_AT
2384
18626_AT

2284
14427_AT
2385
18666_S_AT

2285
14501_AT
2386
18834_AT

2286
14544_AT
2387
18847_AT

2287
14546_S_AT
2388
18896_AT

2288
14570_AT
2389
18899_S_AT

2289
14596_AT
2390
18973_AT

2290
14729_S_AT
2391
18983_S_AT

2291
14874_AT
2392
18988_AT

2292
14888_AT
2393
18998_S_AT

2293
14951_AT
2394
19065_AT

2294
14952_AT
2395
19119_I_AT

2295
14959_AT

19121_AT

2296
14979_AT
2396
19207_AT

2297
15006_AT
2397
19220_AT

2298
15042_AT
2398
19284_AT

2299
15049_AT
2399
19315_AT

2300
15062_AT
2400
19348_AT

2301
15108_S_AT
2401
19403_S_AT

2302
15147_S_AT
2402
19437_S_AT

2303
15175_S_AT
2403
19502_AT

2304
15176_S_AT
2404
19609_AT

2305
15186_S_AT
2405
19645_AT

18696_S_AT
2406
19742_AT

2306
15192_S_AT
2407
19863_AT

2307
15208_S_AT
2408
19873_AT

2308
15324_AT
2409
19891_AT

2309
15371_AT
2410
20004_S_AT

2310
15424_AT
2411
20053_AT

2311
15463_AT
2412
20138_AT

2312
15465_AT
2413
20193_AT

2313
15497_S_AT
2414
20199_AT

2314
15589_S_AT
2415
20220_AT

2315
15636_S_AT
2416
20239_G_AT

2316
15663_S_AT
2417
20297_AT

2317
15770_AT
2418
20324_S_AT

2318
15792_AT
2419
20353_AT

2319
15855_AT
2420
20362_AT

2320
15860_AT
2421
20389_AT

2321
15891_AT
2422
20546_AT

2322
15898_AT
2423
20600_AT

2323
15909_AT
2424
20623_AT

2324
15965_AT
2425
20629_AT

2325
15969_S_AT
2426
20648_S_AT

2326
15975_S—AT
2427
20668_AT

[0211]

8

TABLE 5

2X UP IN SALT, ONLY

12037_at
14570_at
16190_at
18506_at
20648_s_at

12137_at
14578_s_at
16196_at
18605_s_at
20678_at

12153_at
14596_at
16273_at
18626_at
20686_at

12186_at
14646_s_at
16314_at
18666_s_at
20707_s_at

12216_at
14662_f_at
16413_s_at
18747_f_at

12268_at
14668_s_at
16414_at
18782_at

12449_s_at
14729_s_at
16417_s_at
18834_at

12470_at
14874_at
16455_at
18847_at

12476_at
14888_at
16548_s_at
18913_s_at

12487_at
14918_at
16582_s_at
18973_at

12493_g_at
14952_at
16589_s_at
18988_at

12609_at
14959_at
16594_s_at
18998_s_at

12685_at
14986_at
16613_s_at
19065_at

12704_f_at
15006_at
16651_s_at
19068_i_at

12709_f_at
15042_at
16668_at
19123_at

12734_f_at
15047_at
16690_g_at
19177_at

12739_s_at
15062_at
16762_at
19220_at

12750_s_at
15063_at
16820_at
19284_at

12761_s_at
15108_s_at
16873_i_at
19288_at

12819_at
15133_s_at
16987_s_at
19315_at

12845_s_at
15147_s_at
16989_at
19437_s_at

12946_at
15170_s_at
16995_at
19484_s_at

13142_at
15175_s_at
17039_s_at
19502_at

13198_i_at
15182_s_at
17040_s_at
19503_at

13229_s_at
15190_s_at
17400_s_at
19592_at

13275_f_at
15192_s_at
17425_s_at
19645_at

13344_s_at
15324_at
17433_at
19742_at

13370_at
15392_at
17467_at
19835_at

13408_s_at
15424_at
17490_s_at
19873_at

13464_at
15467_at
17529_s_at
19891_at

13472_at
15497_s_at
17543_s_at
19992_at

13526_at
15581_s_at
17566_at
20004_s_at

13614_at
15623_f_at
17595_s_at
20053_at

13652_at
15636_s_at
17744_s_at
20133_i_at

13679_s_at
15646_s_at
17758_at
20138_at

13751_at
15670_s_at
17855_at
20190_at

13918_at
15770_at
17864_at
20199_at

13919_at
15775_at
17876_at
20200_at

13944_at
15778_at
18008_r_at
20297_at

13964_at
15792_at
18013_r_at
20324_s_at

13987_s_at
15855_at
18024_s_at
20335_s_at

13993_s_at
15891_at
18027_at
20353_at

14000_at
15909_at
18053_s_at
20362_at

14032_at
15923_at
18078_at
20385_s_at

14043_at
15969_s_at
18082_at
20389_at

14052_at
15975_s_at
18090_s_at
20402_s_at

14067_at
15995_s_at
18091_at
20450_at

14070_at
15998_s_at
18121_s_at
20468_at

14269_at
16017_at
18264_at
20489_at

14285_at
16050_at
18276_at
20546_at

14427_at
16067_s_at
18300_at
20569_s_at

14501_at
16072_s_at
18367_s_at
20600_at

14540_at
16165_s_at
18471_at
20623_at

[0212]

9

TABLE 9

2X UP SALT, 3 HR ONLY

12037_at
15042_at
16987_s_at
20004_s_at

12137_at
15047_at
16989_at
20053_at

12153_at
15062_at
17039_s_at
20133_i_at

12186_at
15063_at
17040_s_at
20138_at

12216_at
15108_s_at
17425_s_at
20190_at

12268_at
15133_s_at
17433_at
20199_at

12470_at
15147_s_at
17490_s_at
20200_at

12476_at
15170_s_at
17543_s_at
20220_at

12487_at
15175_s_at
17744_s_at
20362_at

12493_g_at
15182_s_at
17864_at
20385_s_at

12609_at
15190_s_at
17876_at
20389_at

12685_at
15192_s_at
18008_r_at
20489_at

12704_f_at
15324_at
18013_r_at
20546_at

12709_f_at
15424_at
18024_s_at
20623_at

12734_f_at
15467_at
18027_at
20648_s_at

12739_s_at
15497_s_at
18053_s_at
20678_at

12750_s_at
15623_f_at
18078_at
20707_s_at

12819_at
15636_s_at
18082_at

12946_at
15646_s_at
18090_s_at

13142_at
15670_s_at
18091_at

13229_s_at
15770_at
18121_s_at

13275_f_at
15775_at
18264_at

13370_at
15778_at
18276_at

13408_s_at
15792_at
18367_s_at

13464_at
15855_at
18471_at

13472_at
15891_at
18506_at

13614_at
15909_at
18605_s_at

13652_at
15923_at
18626_at

13679_s_at
15969_s_at
18666_s_at

13918_at
15975_s_at
18747_f_at

13919_at
15995_s_at
18782_at

13944_at
15998_s_at
18834_at

13987_s_at
16017_at
18847_at

13993_s_at
16050_at
18913_s_at

14000_at
16067_s_at
18973_at

14032_at
16072_s_at
18988_at

14043_at
16165_s_at
19065_at

14052_at
16196_at
19068_i_at

14067_at
16273_at
19123_at

14269_at
16314_at
19177_at

14285_at
16414_at
19220_at

14501_at
16417_s_at
19288_at

14540_at
16455_at
19315_at

14570_at
16548_s_at
19437_s_at

14596_at
16582_s_at
19484_s_at

14668_s_at
16589_s_at
19502_at

14729_s_at
16594_s_at
19503_at

14888_at
16613_s_at
19592_at

14918_at
16651_s_at
19645_at

14952_at
16668_at
19742_at

14959_at
16762_at
19835_at

14986_at
16820_at
19873_at

15006_at
16873_i_at
19891_at

[0213]

10

TABLE 10

2X DOWN SALT, ONLY

12011_s_at
16046_s_at
20239_g_at

12180_at
16060_s_at
20433_at

12265_at
16088_f_at
20629_at

12335_at
16150_s_at
20668_at

12479_at
16166_s_at

12562_at
16316_at

12656_at
16340_at

12813_at
16367_i_at

13003_s_at
16426_at

13052_s_at
16427_at

13094_at
16436_at

13178_at
16489_at

13253_f_at
16502_at

13387_at
16568_s_at

13429_at
16638_s_at

13472_at
16646_s_at

13569_at
17273_at

13686_s_at
17278_at

13718_at
17567_at

13719_at
17868_at

13902_at
17880_s_at

14003_at
17894_at

14144_at
17901_at

14267_at
17942_s_at

14418_at
17960_at

14544_at
17999_at

14546_s_at
18062_at

14636_s_at
18240_s_at

14951_at
18248_s_at

14956_s_at
18267_at

14979_at
18279_s_at

14990_at
18287_at

15040_g_at
18310_at

15049_at
18351_s_at

15115_f_at
18455_at

15137_s_at
18560_at

15148_s_at
18571_at

15176_s_at
18618_s_at

15208_s_at
18896_at

15371_at
18899_s_at

15453_s_at
18967_s_at

15463_at
18983_s_at

15465_at
19119_i_at

15589_s_at
19121_at

15663_s_at
19207_at

15860_at
19348_at

15898_at
19403_s_at

15931_at
19609_at

15965_at
19742_at

15970_s_at
19826_at

15972_s_at
19863_at

16005_s_at
19883_at

16028_at
20193_at

[0214]

11

TABLE 11

OSMOTIC STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX
SEQ
AFFYMETRIX

ID NO:
ID NO:
ID NO:
ID NO:

2428
11994_AT
2505
15918_AT

2429
12028_AT
2506
15999_S_AT

2430
12033_AT
2507
16303_AT

2431
12039_AT
2508
16363_AT

2432
12068_AT
2509
16440_AT

2433
12096_AT
2510
16458_AT

2434
12110_AT
2511
16475_AT

2435
12114_AT
2512
16513_S_AT

2436
12135_AT
2513
16529_AT

2437
12139_AT
2514
16547_S_AT

2438
12189_AT
2515
16553_F_AT

2439
12191_AT
2516
16563_S_AT

2440
12211_AT
2517
16629_S_AT

2441
12223_S_AT
2518
16797_AT

2442
12366_S_AT
2519
16814_AT

12869_S_AT
2520
16832_AT

2443
12381_AT
2521
16976_S_AT

2444
12406_S_AT
2522
17007_AT

2445
12412_AT
2523
17037_S_AT

2446
12453_AT
2524
17054_S_AT

2447
12571_S_AT
2525
17257_s_AT

2448
12662_AT

18725_S_AT

2449
12746_I_AT
2526
17270_AT

2450
12774_AT
2527
17275_I_AT

2451
12787_AT
2528
17376_AT

2452
12847_AT
2529
17378_AT

2453
12848_AT
2530
17468_AT

2454
12895_AT
2531
17481_AT

2455
12911_S_AT
2532
17511_S_AT

2456
12920_AT
2533
17519_S_AT

12921_S_AT
2534
17815_S_AT

2457
13027_AT
2535
17897_AT

2458
13059_AT
2536
17923_S_AT

2459
13075_I_AT
2537
17934_AT

2460
13180_S_AT
2538
17937_S_AT

2461
13255_I_AT
2539
17944_AT

2462
13270_AT
2540
17958_AT

18167_S_AT
2541
18216_AT

2463
13283_S_AT
2542
18227_AT

2464
13382_AT
2543
18284_AT

2465
13386_S_AT
2544
18301_S_AT

2466
13433_AT
2545
18312_S_AT

2467
13482_AT
2546
18326_S_AT

2468
13732_AT
2547
18369_AT

2469
13733_I_AT
2548
18411_AT

2470
13842_AT
2549
18533_AT

2471
13860_S_AT
2550
18576_S_AT

2472
13868_AT
2551
18599_AT

2473
13901_AT
2552
18640_AT

2474
13933_AT
2553
18672_S_AT

2475
13995_AT
2554
18720_S_AT

2476
14062_AT
2555
18768_AT

2477
14118_I_AT
2556
18877_AT

2478
14141_AT
2557
18942_AT

2479
14310_AT
2558
18945_AT

2480
14354_AT
2559
18960_AT

2481
14476_AT
2560
18965_AT

2482
14513_S_AT
2561
19060_AT

2483
14568_S_AT
2562
19164_G_AT

2484
14604_AT
2563
19266_AT

2485
14634_S_AT
2564
19366_S_AT

2486
14660_S_AT
2565
19369_AT

2487
14666_S_AT
2566
19371_AT

2488
14686_S_AT
2567
19386_AT

17464_AT
2568
19412_AT

2489
14726_S_AT
2569
19427_S_AT

2490
14848_S_AT
2570
19622_G_AT

2491
14873_AT
2571
19681_AT

2492
14883_AT
2572
19819_S_AT

2493
15082_AT
2573
19961_S_AT

2494
15121_S_AT
2574
20002_AT

16014_S_AT
2575
20034_I_AT

2495
15168_S_AT
2576
20062_AT

2496
15271_AT
2577
20136_AT

2497
15338_AT
2578
20223_AT

2498
15418_AT
2579
20235_I_AT

2499
15429_AT
2580
20401_AT

2500
15548_AT
2581
20407_AT

2501
15666_S_AT
2582
20470_AT

2502
15672_S_AT
2583
20626_AT

2503
15680_S_AT
2584
20631_S_AT

2504
15867_AT
2585
20647_AT

[0215]

12

TABLE 12

2X UP IN MANNITOL, ONLY

12039_at
16832_at

12068_at
16993_at

12139_at
17037_s_at

12212_at
17054_s_at

12278_at
17083_s_at

12366_s_at
17097_s_at

12453_at
17119_s_at

12556_at
17270_at

12575_s_at
17305_at

12746_i_at
17376_at

12848_at
17378_at

12869_s_at
17449_s_at

12920_at
17481_at

12921_s_at
17533_s_at

13041_s_at
17832_s_at

13059_at
17923_s_at

13241_s_at
17944_at

13255_i_at
18059_i_at

13270_at
18216_at

13382_at
18230_at

13406_at
18255_at

13433_at
18284_at

13550_at
18301_s_at

13672_s_at
18312_s_at

13716_at
18326_s_at

13842_at
18599_at

13933_at
18672_s_at

13995_at
18720_s_at

14062_at
18768_at

14075_at
18814_at

14162_at
18877_at

14208_at
18921_g_at

14217_at
18960_at

14235_at
19060_at

14310_at
19182_at

14431_at
19192_at

14513_s_at
19266_at

14584_at
19369_at

14604_at
19386_at

14673_s_at
19402_at

14856_s_at
19412_at

15207_s_at
19432_s_at

15338_at
19469_at

15406_at
19622_g_at

15418_at
19819_s_at

15591_s_at
19826_at

15666_s_at
20152_at

15680_s_at
20223_at

15866_s_at
20235_i_at

15918_at
20365_s_at

16340_at
20470_at

16553_f_at
20537_at

16797_at
20547_at

[0216]

13

TABLE 13

2X UP IN MANNITOL, 3 HR ONLY

12039_at
17449_s_at

12068_at
17481_at

12139_at
17533_s_at

12212_at
17923_s_at

12278_at
17944_at

12366_s_at
18059_i_at

12453_at
18216_at

12556_at
18230_at

12575_s_at
18255_at

12746_i_at
18301_s_at

12848_at
18312_s_at

12869_s_at
18326_s_at

12920_at
18599_at

12921_s_at
18720_s_at

13041_s_at
18768_at

13059_at
18814_at

13241_s_at
18877_at

13382_at
18921_g_at

13406_at
18960_at

13433_at
19060_at

13550_at
19192_at

13672_s_at
19266_at

13933_at
19369_at

13995_at
19386_at

14062_at
19402_at

14075_at
19412_at

14162_at
19432_s_at

14217_at
19469_at

14310_at
19622_g_at

14431_at
19819_s_at

14513_s_at
20152_at

14584_at
20223_at

14604_at
20235_i_at

14673_s_at
20365_s_at

14856_s_at
20470_at

15207_s_at
20537_at

15338_at

15418_at

15591_s_at

15866_s_at

15918_at

16340_at

16553_f_at

16797_at

16832_at

17037_s_at

17054_s_at

17083_s_at

17097_s_at

17270_at

17305_at

17376_at

17378_at

[0217]

14

TABLE 14

2X DOWN IN MANNITOL, ONLY

12028_at
14897_at
17958_at

12033_at
14918_at
18012_s_at

12110_at
15082_at
18227_at

12114_at
15084_at
18272_at

12189_at
15098_s_at
18331_s_at

12191_at
15105_s_at
18369_at

12211_at
15121_s_at
18411_at

12223_s_at
15126_s_at
18533_at

12268_at
15168_s_at
18576_s_at

12345_at
15271_at
18640_at

12381_at
15429_at
18696_s_at

12406_s_at
15548_at
18945_at

12412_at
15672_s_at
18949_at

12522_at
15753_at
18953_at

12571_s_at
15867_at
18965_at

12662_at
15999_s_at
19164_g_at

12787_at
16001_at
19322_at

12847_at
16021_s_at
19366_s_at

12895_at
16190_at
19371_at

12911_s_at
16260_at
19397_at

13027_at
16303_at
19427_s_at

13075_i_at
16363_at
19681_at

13221_at
16458_s_at
19707_s_at

13262_s_at
16468_at
19839_at

13283_s_at
16475_at
19961_s_at

13386_s_at
16513_s_at
19976_at

13447_s_at
16529_at
19998_at

13482_at
16563_s_at
20002_at

13634_s_at
16690_g_at
20034_i_at

13709_s_at
16814_at
20136_at

13732_at
16847_at
20382_s_at

13733_i_at
16927_s_at
20407_at

13812_s_at
16976_s_at
20529_at

13825_s_at
17007_at
20626_at

13860_s_at
17014_s_at
20631_s_at

13868_at
17016_s_at
20647_at

13901_at
17071_s_at
20699_at

14052_at
17090_s_at

14224_at
17257_s_at

14244_s_at
17275_i_at

14254_s_at
17424_at

14256_f_at
17464_at

14354_at
17468_at

14476_at
17511_s_at

14568_s_at
17519_s_at

14634_s_at
17525_s_at

14646_s_at
17645_s_at

14660_s_at
17741_at

14686_s_at
17815_s_at

14726_s_at
17897_at

14848_s_at
17899_at

14873_at
17934_at

14883_at
17937_s_at

[0218]

15

TABLE 15

COLD & OSOMOTIC STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX
SEQ
AFFYMETRIX

ID NO:
ID NO:
ID NO:
ID NO:

1699
12040_AT
1832
15641_S_AT

1700
12048_AT

18012_S_AT

1701
12054_S_AT
1833
15720_AT

1702
12077_AT
1834
15815_S_AT

1703
12107_I_AT
1835
15817_AT

1704
12113_AT
1836
15837_AT

1705
12154_AT
1837
15841_AT

1706
12171_AT
1838
15866_S_AT

1707
12212_AT

18255_AT

1708
12278_AT
1839
15872_AT

1709
12317_AT

18331_S_AT

1710
12325_AT
1840
15892_AT

1711
12333_AT
1841
15933_AT

1712
12345_AT
1842
15947_AT

1713
12349_S_AT
1843
15959_S_AT

14254_S_AT
1844
16001_AT

14256_F_AT
1845
16052_AT

1714
12356_AT
1846
16161_S_AT

1715
12380_AT
1847
16204_AT

1716
12392_AT
1848
16232_S_AT

1717
12460_S_AT
1849
16252_AT

1718
12556_AT
1850
16260_AT

1719
12575_S_AT
1851
16266_AT

1720
12686_S_AT
1852
16299_AT

1721
12701_I_AT
1853
16365_AT

1722
12754_G_AT
1854
16468_AT

1723
12782_R_AT
1855
16477_AT

1724
12784_AT
1856
16491_AT

1725
12879_S_AT
1857
16523_S_AT

1726
12891_AT
1858
16566_S_AT

16817_S_AT
1859
16570_S_AT

1727
12898_G_AT
1860
16688_AT

1728
12974_AT
1861
16840_AT

1729
12998_AT
1862
16847_AT

1730
13041_S_AT
1863
16893_AT

1731
13124_AT
1864
16896_S_AT

1732
13134_S_AT
1865
16898_S_AT

1733
13144_AT
1866
16912_S_AT

1734
13147_AT
1867
16980_AT

1735
13152_S_AT
1868
16993_AT

1736
13187_I_AT
1869
17008_AT

16981_S_AT
1870
17012_S_AT

1737
13192_S_AT
1871
17014_S_AT

17525_S_AT
1872
17016_S_AT

1738
13212_S_AT
1873
17032_S_AT

1739
13215_S_AT
1874
17050_S_AT

16649_S_AT

17051_S_AT

1740
13241_S_AT
1875
17071_S_AT

1741
13246_AT
1876
17090_S_AT

1742
13262_S_AT

18690_S_AT

1743
13286_S_AT
1877
17097_S_AT

1744
13324_AT
1878
17104_S_AT

1745
13340_S_AT
1879
17119_S_AT

1746
13361_AT
1880
17160_AT

1747
13406_AT
1881
17305_AT

1748
13441_S_AT
1882
17424_AT

1749
13513_AT
1883
17449_S_AT

1750
13550_AT
1884
17452_G_AT

1751
13573_AT
1885
17540_S_AT

1752
13577_S_AT
1886
17552_S_AT

1753
13606_AT
1887
17571_AT

1754
13609_AT
1888
17589_AT

1755
13625_S_AT
1889
17641_G_AT

1756
13626_AT
1890
17741_AT

1757
13634_S_AT

18098_AT

1758
13672_S_AT
1891
17766_AT

18916_S_AT
1892
17873_S_AT

1759
13709_S_AT
1893
17904_AT

1760
13736_AT
1894
17920_S_AT

1761
13775_AT
1895
17925_AT

1762
13810_AT
1896
17943_AT

1763
13812_S_AT
1897
18059_I_AT

1764
13825_S_AT
1898
18230_AT

1765
14015_S_AT
1899
18263_AT

14016_S_AT
1900
18272_AT

1766
14029_AT
1901
18540_AT

1767
14036_AT
1902
18608_AT

1768
14051_AT
1903
18647_AT

1769
14060_AT
1904
18662_S_AT

1770
14064_AT
1905
18664_AT

1771
14066_AT
1906
18695_S_AT

1772
14075_AT
1907
18704_AT

1773
14094_S_AT
1908
18814_AT

19999_S_AT
1909
18907_S_AT

1774
14096_AT
1910
18921_G_AT

1775
14104_AT
1911
18924_AT

1776
14123_S_AT
1912
18949_AT

1777
14126_S_AT

19707_S_AT

1778
14131_AT
1913
18995_AT

1779
14136_AT
1914
19017_AT

1780
14139_AT
1915
19034_AT

14140_AT
1916
19063_AT

1781
14162_AT
1917
19142_AT

14217_AT
1918
19158_AT

1782
14178_AT
1919
19180_AT

1783
14201_AT
1920
19187_AT

1784
14208_AT
1921
19192_AT

1785
14235_AT
1922
19195_AT

1786
14242_S_AT
1923
19199_AT

1787
14431_AT
1924
19231_AT

1788
14480_AT
1925
19263_AT

1789
14497_AT
1926
19308_AT

1790
14553_AT
1927
19322_AT

1791
14584_AT
1928
19365_S_AT

1792
14600_AT
1929
19372_AT

1793
14673_S_AT
1930
19389_AT

19432_S_AT
1931
19392_AT

1794
14681_G_AT
1932
19397_AT

1795
14699_AT
1933
19400_AT

1796
14751_AT
1934
19402_AT

1797
14762_AT
1935
19458_AT

1798
14828_S_AT
1936
19469_AT

1799
14856_S_AT
1937
19473_AT

1800
14882_AT
1938
19597_S_AT

1801
14897_AT
1939
19710_S_AT

1802
14978_AT
1940
19830_AT

1803
14985_S_AT
1941
19839_AT

1804
15031_AT
1942
19840_S_AT

1805
15084_AT
1943
19853_AT

1806
15096_AT
1944
19860_AT

1807
15105_S_AT
1945
19880_AT

1808
15110_S_AT
1946
19889_AT

1809
15111_S_AT
1947
19898_AT

1810
15120_S_AT
1948
19914_AT

1811
15126_S_AT
1949
19924_AT

1812
15142_S_AT
1950
19949_AT

1813
15144_S_AT
1951
19976_AT

1814
15184_S_AT
1952
19998_AT

1815
15198_S_AT
1953
20030_AT

1816
15203_S_AT
1954
20151_AT

1817
15207_S_AT
1955
20152_AT

1818
15240_AT
1956
20187_AT

1819
15366_AT
1957
20214_I_AT

1820
15398_AT
1958
20269_AT

1821
15406_AT
1959
20271_AT

1822
15448_AT
1960
20273_AT

1823
15466_AT
1961
20299_AT

1824
15481_AT
1962
20323_AT

1825
15484_AT
1963
20429_S_AT

1826
15549_AT
1964
20457_AT

1827
15591_S_AT
1965
20480_S_AT

1828
15606_S_AT
1966
20529_AT

1829
15614_S_AT
1967
20547_AT

16927_S_AT
1968
20555_S_AT

1830
15629_S_AT
1969
20699_AT

1831
15633_S_AT

[0219]

16

TABLE 16

2X UP IN MANNITOL & COLD, ONLY

12345_at
17066_s_at

12784_at
17540_s_at

13153_r_at
17567_at

13212_s_at
17766_at

13215_s_at
17904_at

13246_at
17920_s_at

13262_s_at
17943_at

13361_at
18263_at

13625_s_at
18351_s_at

13764_at
18662_s_at

13810_at
18670_g_at

14015_s_at
18695_s_at

14016_s_at
18704_at

14060_at
18729_at

14096_at
18995_at

14123_s_at
19158_at

14139_at
19473_at

14219_at
19710_s_at

14248_at
19883_at

14254_s_at
19889_at

14256_f_at
20030_at

14609_at
20269_at

14636_s_at
20271_at

14681_g_at
20299_at

14699_at
20429_s_at

14704_s_at
20438_at

14828_s_at
20480_s_at

14882_at

15110_s_at

15184_s_at

15448_at

15629_s_at

15720_at

15846_at

15947_at

16161_s_at

16365_at

16427_at

16566_s_at

16570_s_at

16649_s_at

16688_at

16712_at

16817_s_at

16840_at

16893_at

16912_s_at

16916_s_at

16927_s_at

16981_s_at

17012_s_at

17014_s_at

17051_s_at

[0220]

17

TABLE 17

2X DOWN COLD & MANNITOL, ONLY

12040_at
14553_at
17873_s_at

12048_at
14612_at
17925_at

12054_s_at
14751_at
18098_at

12077_at
14762_at
18540_at

12107_i_at
14978_at
18608_at

12113_at
14985_s_at
18647_at

12154_at
15031_at
18664_at

12171_at
15096_at
18690_s_at

12317_at
15111_s_at
18725_s_at

12325_at
15120_s_at
18924_at

12333_at
15142_s_at
19017_at

12356_at
15198_s_at
19034_at

12380_at
15203_s_at
19063_at

12392_at
15240_at
19141_at

12460_s_at
15366_at
19142_at

12686_s_at
15392_at
19180_at

12701_i_at
15398_at
19187_at

12782_r_at
15466_at
19195_at

12879_s_at
15481_at
19199_at

12898_g_at
15484_at
19231_at

12974_at
15549_at
19308_at

12998_at
15623_f_at
19372_at

13144_at
15815_s_at
19392_at

13147_at
15817_at
19400_at

13152_s_at
15841_at
19458_at

13192_s_at
15892_at
19597_s_at

13286_s_at
15933_at
19762_at

13324_at
15959_s_at
19830_at

13340_s_at
16052_at
19853_at

13441_s_at
16204_at
19869_at

13513_at
16252_at
19880_at

13573_at
16266_at
19898_at

13606_at
16299_at
19914_at

13609_at
16477_at
19924_at

13626_at
16491_at
19949_at

13736_at
16561_s_at
20151_at

13775_at
16645_s_at
20187_at

14029_at
16898_s_at
20214_i_at

14036_at
16980_at
20273_at

14051_at
17008_at
20323_at

14064_at
17104_s_at
20457_at

14066_at
17160_at
20555_s_at

14094_s_at
17317_at

14104_at
17400_s_at

14126_s_at
17452_g_at

14131_at
17477_s_at

14136_at
17500_s_at

14178_at
17552_s_at

14192_at
17571_at

14201_at
17572_s_at

14242_s_at
17589_at

14480_at
17641_g_at

14497_at
17855_at

[0221]

18

TABLE 18

COLD & SALINE STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX
SEQ
AFFYMETRD

ID NO:
ID NO:
ID NO:
ID NO:

1970
12021_AT
2098
16120_S_AT

1971
12037_AT
2099
16126_S_AT

1972
12094_AT
2100
16150_S_AT

1973
12098_AT
2101
16159_S_AT

1974
12128_AT
2102
16230_AT

1975
12148_AT
2103
16306_AT

1976
12151_AT
2104
16367_I_AT

1977
12357_S_AT
2105
16417_S_AT

1978
12394_AT

18083_R_AT

1979
12472_S_AT
2106
16418_S_AT

1980
12475_AT
2107
16423_AT

1981
12482_S_AT
2108
16449_S_AT

1982
12490_AT
2109
16484_S_AT

1983
12505_S_AT
2110
16489_AT

1984
12531_AT
2111
16565_S_AT

1985
12540_S_AT
2112
16596_S_AT

1986
12541_AT
2113
16600_S_AT

1987
12577_AT
2114
16603_S_AT

1988
12594_AT
2115
16638_S_AT

1989
12629_AT
2116
16642_S_AT

1990
12642_AT
2117
16763_AT

1991
12656_AT
2118
16914_S_AT

1992
12660_AT
2119
16968_AT

1993
12712_F_AT
2120
16983_AT

1994
12725_R_AT
2121
16989_AT

1995
12745_AT
2122
17002_AT

1996
12777_I_AT
2123
17015_S_AT

1997
12790_S_AT
2124
17040_S_AT

1998
12798_AT

18913_S_AT

1999
12801_AT
2125
17232_AT

2000
12855_F_AT
2126
17380_AT

2001
12887_S_AT
2127
17394_S_AT

2002
12933_R_AT

20640_S_AT

2003
12951_AT
2128
17398_AT

2004
13005_AT
2129
17448_AT

2005
13015_S_AT
2130
17485_S_AT

2006
13115_AT
2131
17490_S_AT

2007
13178_AT
2132
17499_S_AT

2008
13228_AT
2133
17505_S_AT

2009
13236_S_AT
2134
17516_S_AT

16646_S_AT
2135
17529_S_AT

2010
13266_S_AT
2136
17543_S_AT

15211_S_AT
2137
17593_R_AT

2011
13275_F_AT

19858_S_AT

2012
13335_AT
2138
17609_AT

2013
13362_S_AT
2139
17698_AT

2014
13428_AT
2140
17836_AT

2015
13464_AT
2141
17886_AT

2016
13480_AT
2142
17896_AT

2017
13538_AT
2143
17901_AT

2018
13544_AT
2144
17902_S_AT

2019
13549_AT
2145
17913_S_AT

2020
13565_AT
2146
17924_AT

2021
13580_AT
2147
17954_S_AT

2022
13588_AT
2148
17960_AT

2023
13649_AT
2149
17991_G_AT

2024
13652_AT

18967_S_AT

2025
13679_S_AT
2150
17999_AT

2026
13696_AT
2151
18057_I_AT

2027
13702_S_AT
2152
18078_AT

2028
13751_AT
2153
18091_AT

2029
13919_AT
2154
18168_S_AT

2030
13943_AT
2155
18252_AT

2031
13950_S_AT
2156
18267_AT

2032
14050_AT
2157
18300_AT

2033
14055_S_AT
2158
18308_I_AT

16166_S_AT
2159
18328_AT

2034
14067_AT
2160
18354_AT

2035
14078_AT
2161
18402_AT

2036
14110_I_AT
2162
18416_AT

2037
14144_AT
2163
18455_AT

2038
14232_AT
2164
18459_AT

2039
14285_AT
2165
18571_AT

2040
14346_AT
2166
18604_AT

2041
14432_AT

19181_S_AT

2042
14468_AT
2167
18644_AT

2043
14479_AT
2168
18745_F_AT

2044
14524_S_AT

19611_S_AT

2045
14608_AT
2169
18782_AT

2046
14621_AT
2170
18881_AT

2047
14635_S_AT
2171
18904_S_AT

17128_S_AT
2172
18914_S_AT

2048
14640_S_AT
2173
18963_AT

2049
14643_S_AT
2174
19068_I_AT

2050
14663_S_AT
2175
19078_AT

2051
14668_S_AT
2176
19171_AT

2052
14688_S_AT
2177
19177_AT

18279_S_AT
2178
19394_AT

2053
14737_S_AT
2179
19411_AT

2054
14768_AT
2180
19415_AT

2055
14875_AT
2181
19466_S_AT

2056
14911_S_AT
2182
19484_S_AT

17056_S_AT
2183
19549_S_AT

2057
14924_AT
2184
19592_AT

2058
14956_S_AT
2185
19633_AT

15148_S_AT
2186
19641_AT

18673_AT
2187
19669_AT

2059
14964_AT
2188
19672_AT

2060
15022_AT
2189
19684_AT

2061
15040_G_AT
2190
19692_AT

2062
15047_AT
2191
19746_AT

2063
15063_AT
2192
19835_AT

2064
15085_S_AT
2193
19848_S_AT

2065
15123_S_AT
2194
19892_AT

2066
15133_S_AT
2195
19904_AT

2067
15137_S_AT
2196
19936_AT

2068
15153_S_AT
2197
19974_S_AT

2069
15170_S_AT
2198
19994_AT

2070
15172_S_AT
2199
20005_S_AT

2071
15182_S_AT
2200
20022_AT

2072
15190_S_AT
2201
20032_AT

2073
15241_S_AT
2202
20044_AT

2074
15389_AT
2203
20049_AT

2075
15453_S_AT
2204
20081_AT

2076
15495_AT
2205
20133_I_AT

2077
15496_AT
2206
20155_S_AT

2078
15519_S_AT
2207
20163_S_AT

2079
15562_AT
2208
20200_AT

2080
15580_S_AT
2209
20296_S_AT

2081
15582_S_AT
2210
20336_AT

2082
15638_S_AT
2211
20341_AT

18751_F_AT
2212
20372_AT

2083
15646_S_AT
2213
20385_S_AT

2084
15647_S_AT
2214
20433_AT

2085
15654_S_AT
2215
20489_AT

2086
15655_S_AT
2216
20525_AT

2087
15658_S_AT
2217
20543_AT

2088
15670_S_AT
2218
20565_AT

2089
15775_AT
2219
20570_AT

2090
15798_AT
2220
20576_AT

2091
15930_AT
2221
20577_AT

2092
15931_AT
2222
20609_AT

2093
15949_S_AT
2223
20646_AT

2094
16017_AT
2224
20672_AT

2095
16053_I_AT
2225
20707_S_AT

2096
16078_S_AT
2226
20720_AT

2097
16086_S_AT

[0222]

19

TABLE 19

2X UP IN SALT & COLD, ONLY

12004_at
15495_at
18745_f_at

12098_at
15496_at
18904_s_at

12148_at
15519_s_at
18914_s_at

12251_at
15580_s_at
18929_s_at

12357_s_at
15582_s_at
18946_at

12394_at
15776_at
18963_at

12457_at
15798_at
19078_at

12505_s_at
15910_at
19137_at

12522_at
15931_at
19141_at

12541_at
15937_at
19411_at

12594_at
15949_s_at
19641_at

12605_at
15972_s_at
19672_at

12697_at
16048_at
19684_at

12745_at
16086_s_at
19692_at

12781_at
16120_s_at
19746_at

12798_at
16126_s_at
19762_at

12855_f_at
16150_s_at
19869_at

12945_at
16159_s_at
19894_at

12951_at
16230_at
19904_at

13005_at
16306_at
19936_at

13015_s_at
16418_s_at
19994_at

13115_at
16423_at
20005_s_at

13146_s_at
16449_s_at
20031_at

13335_at
16565_s_at
20044_at

13447_s_at
16603_s_at
20382_s_at

13480_at
16763_at
20406_at

13544_at
16968_at
20421_at

13549_at
16983_at
20525_at

13580_at
17002_at
20543_at

13649_at
17015_s_at
20565_at

13943_at
17019_s_at
20570_at

13950_s_at
17078_s_at
20640_s_at

14110_i_at
17232_at
20646_at

14144_at
17317_at
20720_at

14224_at
17394_s_at

14432_at
17516_s_at

14468_at
17585_s_at

14479_at
17609_at

14524_s_at
17698_at

14640_s_at
17836_at

14643_s_at
17896_at

14735_s_at
17899_at

14737_s_at
17902_s_at

14768_at
17960_at

14784_at
17963_at

14924_at
18168_s_at

15064_at
18252_at

15127_s_at
18267_at

15186_s_at
18308_i_at

15189_s_at
18354_at

15255_at
18402_at

15389_at
18459_at

15482_at
18484_at

[0223]

20

TABLE 20

2X DOWN IN COLD & SALT, ONLY

12021_at

12094_at

12128_at

12151_at

12332_s_at

12472_s_at

12475_at

12482_s_at

12490_at

12531_at

12540_s_at

12577_at

12629_at

12642_at

12660_at

12676_s_at

12712_f_at

12725_r_at

12777_i_at

12790_s_at

12801_at

12887_s_at

12933_r_at

13153_r_at

13228_at

13362_s_at

13428_at

13538_at

13565_at

13588_at

13696_at

13702_s_at

13716_at

13764_at

14050_at

14055_s_at

14069_at

14078_at

14232_at

14346_at

14608_at

14609_at

14621_at

14635_s_at

14663_s_at

14688_s_at

14691_at

14704_s_at

14875_at

14911_s_at

14964_at

15022_at

15085_s_at

15123_s_at

15153_s_at

15172_s_at

15190_s_at

15211_s_at

15241_s_at

15437_at

15562_at

15638_s_at

15647_s_at

15654_s_at

15655_s_at

15658_s_at

15695_s_at

15846_at

15930_at

16053_i_at

16078_s_at

16229_at

16465_at

16484_s_at

16596_s_at

16600_s_at

16642_s_at

16914_s_at

17027_s_at

17066_s_at

17083_s_at

17128_s_at

17380_at

17398_at

17448_at

17485_s_at

17490_s_at

17499_s_at

17505_s_at

17514_s_at

17593_r_at

17886_at

17913_s_at

17924_at

17954_s_at

17991_g_at

18057_i_at

18069_at

18328_at

18416_at

18604_at

18644_at

18881_at

19171_at

19181_s_at

19182_at

19394_at

19415_at

19466_s_at

19549_s_at

19592_at

19633_at

19669_at

19848_s_at

19858_s_at

19878_at

19892_at

19974_s_at

20022_at

20032_at

20049_at

20081_at

20155_s_at

20163_s_at

20296_s_at

20336_at

20341_at

20365_s_at

20372_at

20489_at

20491_at

20576_at

20577_at

20609_at

20672_at

[0224]

21

TABLE 21

OSMOTIC & SALINE STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX
SEQ
AFFYMETRIX

ID NO:
ID NO:
ID NO:
ID NO:

2586
12126_S_AT
2644
16340_AT

2587
12137_AT
2645
16450_S_AT

2588
12227_AT
2646
16500_AT

2589
12239_AT
2647
16524_AT

2590
12268_AT
2648
16533_AT

2591
12369_AT
2649
16690_G_AT

2592
12476_AT
2650
16762_AT

2593
12484_G_AT
2651
16819_AT

2594
12494_AT
2652
16873_I_AT

2595
12644_AT
2653
16972_AT

2596
12645_AT
2654
16991_AT

2597
12796_S_AT
2655
17099_S_AT

2598
12819_AT
2656
17339_AT

2599
12841_AT
2657
17397_S_AT

2600
12852_S_AT
2658
17419_AT

19455_S_AT
2659
17460_AT

2601
13084_AT
2660
17554_S_AT

2602
13171_AT
2661
17939_AT

2603
13174_R_AT
2662
18013_R_AT

2604
13596_AT

18178_S_AT

2605
13807_AT
2663
18024_S_AT

2606
13977_AT
2664
18032_I_AT

2607
13999_AT
2665
18054_AT

2608
14052_AT
2666
18151_AT

2609
14293_AT
2667
18281_AT

2610
14335_AT
2668
18445_AT

2611
14486_AT
2669
18520_AT

2612
14506_AT
2670
18583_AT

2613
14518_AT
2671
18663_S_AT

2614
14540_AT
2672
18753_S_AT

2615
14578_S_AT
2673
18876_AT

2616
14646_S_AT
2674
18938_G_AT

2617
14662_F_AT
2675
18971_AT

15962_S_AT
2676
18977_AT

2618
14901_AT
2677
18981_AT

2619
14918_AT
2678
19099_AT

2620
14986_AT
2679
19196_AT

2621
15053_S_AT
2680
19376_AT

2622
15179_S_AT
2681
19409_AT

2623
15252_G_AT
2682
19503_AT

2624
15280_AT
2683
19826_AT

2625
15467_AT
2684
19847_S_AT

2626
15607_S_AT
2685
19930_AT

2627
15625_S_AT
2686
19992_AT

2628
15703_I_AT
2687
20096_AT

2629
15827_AT
2688
20108_AT

2630
15863_AT
2689
20256_S_AT

2631
15923_AT
2690
20290_S_AT

2632
15946_S_AT
2691
20298_AT

2633
16005_S_AT
2692
20305_AT

2634
16073_F_AT
2693
20322_AT

2635
16114_S_AT
2694
20333_AT

2636
16127_S_AT
2695
20402_S_AT

18744_F_AT
2696
20424_AT

2637
16190_AT
2697
20446_S_AT

2638
16196_AT
2698
20450_AT

2639
16236_G_AT
2699
20468_AT

19531_AT
2700
20569_S_AT

2640
16310_AT
2701
20639_AT

2641
16316_AT
2702
20678_AT

2642
16334_S_AT
2703
20686_AT

2643
16335_AT

[0225]

22

TABLE 22

2X UP IN SALT & MANNITOL, ONLY

12126_s_at

12227_at

12369_at

12521_at

12644_at

12645_at

12724_f_at

12795_at

12796_s_at

12841_at

12852_s_at

12958_at

13014_at

13174_r_at

13211_s_at

13596_at

13640_at

13789_at

13977_at

13999_at

14069_at

14083_at

14089_at

14293_at

14675_s_at

15053_s_at

15058_s_at

15252_g_at

15280_at

15437_at

15607_s_at

15625_s_at

15827_at

15863_at

15880_at

16005_s_at

16031_at

16073_f_at

16316_at

16334_s_at

16335_at

16450_s_at

16500_at

16524_at

16533_at

16597_s_at

16819_at

17085_s_at

17099_s_at

17339_at

17419_at

17442_i_at

17514_s_at

17548_s_at

17554_s_at

17961_at

18032 i at

18054_at

18151_at

18167_s_at

18281_at

18520_at

18663_s_at

18744 f at

18753_s_at

18789_at

18876_at

18909_s_at

18938gat

18977 at

19099_at

19108_at

19135_at

19227_at

19376_at

19429 at

19455_s_at

19531_at

19789_s_at

19878_at

20017_at

20096 at

20256_s_at

20290_s_at

20305_at

20322_at

20333_at

20420_at

20424_at

20689_s_at

[0226]

23

TABLE 23

2X DOWN IN MANNITOL & SALT, ONLY

12239_at

12251_at

12476_at

12484_g_at

12494_at

12561_at

12647_s_at

12719_f_at

12819_at

12841_at

13084_at

13171_at

13172_s_at

13435_at

13807_at

14250_r_at

14335_at

14486_at

14506_at

14518_at

14901_at

15046_s_at

15179_s_at

15451_at

15703_i_at

15946_s_at

16014_s_at

16114_s_at

16310_at

16342_at

16712_at

16762_at

16972_at

16991_at

17397_s_at

17408_at

17460_at

17775_at

17939_at

18445_at

18583_at

18751_f_at

18971_at

18981_at

19156_s_at

19196_at

19359_s_at

19409_at

19503_at

19713_at

19718_at

19847_s_at

19930_at

20108_at

20298_at

20421_at

20432_at

20446_s_at

20639_at

[0227]

24

TABLE 24

COLD, OSMOTIC & SALINE RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX
SEQ
AFFYMETRIX

ID NO:
ID NO:
ID NO:
ID NO:

1262
12004_AT
1470
16141_S_AT

1263
12023_S_AT
1471
16144_S_AT

1264
12078_AT
1472
16163_S_AT

1265
12115_AT
1473
16173_S_AT

1266
12118_AT
1474
16229_AT

1267
12150_AT
1475
16298_AT

1268
12251_AT
1476
16301_S_AT

1269
12271_S_AT
1477
16322_AT

1270
12276_AT
1478
16342_AT

1271
12332_S_AT
1479
16351_AT

13211_S_AT
1480
16412_S_AT

1272
12338_AT
1481
16422_AT

1273
12400_AT
1482
16427_AT

1274
12430_AT
1483
16438_AT

1275
12457_AT
1484
16474_S_AT

1276
12521_AT
1485
16482_S_AT

1277
12522_AT
1486
16485_S_AT

1278
12530_AT

18052_S_AT

1279
12536_S_AT
1487
16493_AT

1280
12538_AT
1488
16534_S_AT

1281
12561_AT
1489
16555_S_AT

1282
12574_AT
1490
16561_S_AT

19019_I_AT

17572_S_AT

1283
12595_AT
1491
16592_S_AT

1284
12606_AT
1492
16615_S_AT

1285
12609_AT
1493
16637_S_AT

1286
12622_AT
1494
16692_AT

1287
12630_AT
1495
16712_AT

1288
12647_S_AT
1496
16789_AT

1289
12676_S_AT
1497
16818_S_AT

1290
12697_AT
1498
16971_S_AT

1291
12698_AT
1499
17018_S_AT

1292
12719_F_AT
1500
17019_S_AT

1293
12724_F_AT
1501
17029_S_AT

15871_S_AT
1502
17041_S_AT

16597_S_AT
1503
17047_S_AT

1294
12749_AT
1504
17066_S_AT

1295
12765_AT
1505
17085_S_AT

1296
12769_AT
1506
17089_S_AT

1297
12781_AT
1507
17179_AT

1298
12785_AT
1508
17180_AT

1299
12792_S_AT
1509
17228_AT

1300
12795_AT
1510
17252_AT

1301
12805_S_AT
1511
17317_AT

1302
12857_AT
1512
17338_AT

1303
12883_S_AT
1513
17384_AT

1304
12909_S_AT
1514
17387_S_AT

16539_S_AT
1515
17400_S_AT

1305
12932_S_AT
1516
17407_S_AT

15605_S_AT
1517
17408_AT

1306
12945_AT
1518
17413_S_AT

1307
12958_AT
1519
17416_AT

1308
12964_AT
1520
17425_S_AT

1309
12968_AT
1521
17440_I_AT

1310
12972_AT
1522
17442_I_AT

1311
12989_S_AT
1523
17473_AT

1312
13004_AT
1524
17484_AT

1313
13014_AT
1525
17514_S_AT

1314
13025_AT
1526
17520_S_AT

1315
13036_AT
1527
17533_S_AT

1316
13099_S_AT
1528
17548_S_AT

1317
13136_AT

19614_AT

1318
13146_S_AT
1529
17549_S_AT

13239_S_AT
1530
17555_S_AT

1319
13153_R_AT
1531
17567_AT

1320
13159_AT
1532
17654_AT

1321
13176_AT
1533
17693_AT

1322
13217_S_AT
1534
17697_AT

17500_S_AT
1535
17722_AT

1323
13225_S_AT
1536
17752_AT

15997_S_AT
1537
17755_AT

1324
13230_S_AT
1538
17775_AT

15972_S_AT
1539
17832_S_AT

1325
13279_S_AT
1540
17840_S_AT

17477_S_AT
1541
17843_SAT

1326
13280_S_AT
1542
17855_AT

20301_S_AT
1543
17860_AT

1327
13282_S_AT
1544
17869_AT

17027_S_AT
1545
17888_AT

1328
13426_AT
1546
17899_AT

1329
13432_AT
1547
17929_S_AT

1330
13435_AT
1548
17930_S_AT

1331
13447_S_AT
1549
17932_S_AT

1332
13474_AT
1550
17936_S_AT

1333
13511_AT

18670_G_AT

1334
13546_AT
1551
17957_AT

1335
13547_S_AT
1552
17961_AT

1336
13548_AT
1553
17962_AT

1337
13555_AT
1554
17963_AT

1338
13587_AT
1555
17971_S_AT

1339
13595_AT
1556
17975_AT

1340
13610_S_AT

18742_F_AT

1341
13627_AT
1557
18016_R_AT

1342
13640_AT
1558
18069_AT

1343
13645_AT
1559
18122_AT

1344
13647_AT
1560
18140_AT

1345
13706_S_AT
1561
18199_AT

19701_S_AT
1562
18224_S_AT

1346
13716_AT
1563
18225_AT

18228_AT
1564
18235_AT

1347
13725_AT
1565
18259_S_AT

1348
13764_AT
1566
18265_AT

1349
13771_AT
1567
18270_AT

1350
13789_AT
1568
18280_AT

1351
13916_AT
1569
18289_AT

1352
13965_S_AT
1570
18296_AT

1353
13967_AT
1571
18298_AT

1354
14028_AT
1572
18314_I_AT

1355
14039_AT
1573
18318_AT

1356
14046_AT
1574
18325_AT

1357
14049_AT
1575
18351_S_AT

1358
14069_AT
1576
18471_AT

1359
14077_AT
1577
18482_S_AT

1360
14080_AT
1578
18484_AT

1361
14083_AT
1579
18560_AT

1362
14089_AT
1580
18564_AT

1363
14090_I_AT
1581
18590_AT

1364
14097_AT
1582
18594_AT

1365
14116_AT
1583
18595_AT

1366
14151_AT
1584
18596_AT

14219_AT
1585
18629_S_AT

1367
14170_AT
1586
18637_AT

1368
14172_AT
1587
18661_AT

1369
14192_AT
1588
18668_AT

1370
14224_AT
1589
18699_I_AT

1371
14227_AT
1590
18747_F_AT

1372
14244_S_AT
1591
18761_AT

14245_AT
1592
18833_AT

14645_S_AT
1593
18875_S_AT

15974_G_AT
1594
18894_AT

1373
14248_AT
1595
18936_AT

1374
14250_R_AT
1596
18946_AT

1375
14367_AT
1597
18953_AT

1376
14381_AT
1598
18955_AT

1377
14384_AT
1599
18972_AT

1378
14398_S_AT
1600
19008_S_AT

1379
14487_AT
1601
19108_AT

1380
14582_AT
1602
19123_AT

1381
14597_AT
1603
19135_AT

1382
14609_AT
1604
19137_AT

1383
14612_AT
1605
19141_AT

19267_S_AT
1606
19152_AT

1384
14614_AT
1607
19156_S_AT

1385
14636_S_AT
1608
19182_AT

1386
14644_S_AT
1609
19186_S_AT

14658_S_AT
1610
19214_AT

14659_S_AT
1611
19216_AT

15964_S_AT
1612
19227_AT

1387
14675_S_AT
1613
19243_AT

1388
14691_AT
1614
19288_AT

14709_AT
1615
19359_S_AT

1389
14704_S_AT
1616
19368_AT

15846_AT
1617
19379_AT

1390
14705_I_AT
1618
19380_S_AT

1391
14733_S_AT
1619
19398_AT

1392
14735_S_AT
1620
19421_AT

1393
14779_AT
1621
19424_AT

1394
14784_AT
1622
19429_AT

1395
14923_AT
1623
19430_AT

1396
14947_AT
1624
19450_AT

1397
14950_AT
1625
19457_AT

1398
14990_AT
1626
19467_AT

1399
14998_AT
1627
19516_AT

1400
15005_S_AT
1628
19545_AT

1401
15018_AT
1629
19564_AT

1402
15045_AT
1630
19577_AT

1403
15046_S_AT
1631
19593_AT

1404
15052_AT
1632
19602_AT

1405
15058_S_AT
1633
19618_AT

1406
15064_AT
1634
19638_AT

1407
15088_S_AT
1635
19640_AT

1408
15098_S_AT
1636
19646_S_AT

1409
15103_S_AT
1637
19656_S_AT

1410
15109_S_AT
1638
19670_AT

1411
15124_S_AT
1639
19696_AT

1412
15127_S_AT
1640
19713_AT

1413
15145_S_AT
1641
19718_AT

1414
15154_S_AT
1642
19722_S_AT

1415
15161_S_AT
1643
19749_AT

1416
15189_S_AT
1644
19755_AT

1417
15214_S_AT
1645
19762_AT

1418
15255_AT
1646
19789_S_AT

1419
15356_AT
1647
19815_AT

1420
15357_AT
1648
19843_AT

1421
15364_AT
1649
19869_AT

1422
15392_AT
1650
19878_AT

1423
15403_S_AT
1651
19883_AT

1424
15437_AT
1652
19894_AT

1425
15451_AT
1653
19926_AT

1426
15476_AT
1654
19944_AT

1427
15482_AT
1655
19968_AT

1428
15483_S_AT
1656
19977_AT

1429
15521_S_AT
1657
19982_AT

1430
15522_I_AT
1658
19987_AT

1431
15531_I_AT
1659
19991_AT

1432
15573_AT
1660
20015_AT

1433
15581_S_AT
1661
20017_AT

1434
15586_S_AT
1662
20031_AT

1435
15594_S_AT
1663
20040_AT

1436
15609_S_AT
1664
20042_S_AT

1437
15611_S_AT
1665
20060_AT

1438
15621_F_AT

20438_AT

1439
15623_F_AT
1666
20089_AT

1440
15669_S_AT
1667
20118_AT

1441
15695_S_AT
1668
20144_AT

1442
15702_S_AT
1669
20149_AT

1443
15753_AT
1670
20179_AT

1444
15761_AT
1671
20190_AT

1445
15776_AT
1672
20194_AT

1446
15778_AT
1673
20219_AT

1447
15839_AT
1674
20245_S_AT

1448
15842_AT
1675
20263_AT

1449
15857_S_AT
1676
20308_S_AT

1450
15859_AT
1677
20335_S_AT

1451
15880_AT
1678
20338_AT

1452
15886_AT
1679
20345_AT

1453
15906_S_AT
1680
20365_S_AT

1454
15910_AT
1681
20382_S_AT

1455
15937_AT
1682
20390_S_AT

1456
15957_AT
1683
20395_AT

1457
15970_S_AT
1684
20420_AT

1458
15985_AT
1685
20421_AT

1459
16010_S_AT
1686
20432_AT

16011_S_AT
1687
20437_AT

17078_S_AT
1688
20442_I_AT

1460
16021_S_AT
1689
20463_S_AT

1461
16031_AT
1690
20491_AT

1462
16038_S_AT
1691
20537_AT

1463
16045_S_AT
1692
20573_AT

1464
16046_S_AT
1693
20636_AT

1465
16048_AT
1694
20638_AT

1466
16061_S_AT
1695
20641_AT

1467
16082_S_AT
1696
20658_S_AT

1468
16111_F_AT
1697
20689_S_AT

1469
16115_S_AT
1698
20698_S_AT

[0228]

25

TABLE 25

2X UP IN COLD, SALT & MANNITOL

12023_s_at

12332_s_at

12530_at

12536_s_at

12574_at

12595_at

12698_at

12749_at

12765_at

12769_at

12785_at

12857_at

12964_at

12972_at

12989_s_at

13004_at

13025_at

13036_at

13099_s_at

13136_at

13176_at

13220_s_at

13225_s_at

13230_s_at

13239_s_at

13426_at

13474_at

13548_at

13555_at

13595_at

13627_at

13645_at

13647_at

13706_s_at

13965_s_at

13967_at

14080_at

14090_i_at

14097_at

14116_at

14151_at

14172_at

14192_at

14244_s_at

14245_at

14367_at

14398_s_at

14582_at

14614_at

14644_s_at

14645_s_at

14658_s_at

14659_s_at

14733_s_at

14923_at

14990_at

15005_s_at

15018_at

15052_at

15088_s_at

15098_s_at

15103_s_at

15145_s_at

15154_s_at

15161_s_at

15214_s_at

15356_at

15521_s_at

15573_at

15586_s_at

15609_s_at

15611_s_at

15621_f_at

15669_s_at

15695_s_at

15753_at

15761_at

15857_s_at

15871_s_at

15964_s_at

15970_s_at

15974_g_at

15997_s_at

16011_s_at

16021_s_at

16038_s_at

16046_s_at

16082_s_at

16111_f_at

16115_s_at

16127_s_at

16141_s_at

16144_s_at

16163_s_at

16236_g_at

16301_s_at

16322_at

16422_at

16474_s_at

16482_s_at

16485_s_at

16555_s_at

16561_s_at

16592_s_at

16637_s_at

17041_s_at

17047_s_at

17179_at

17180_at

17252_at

17384_at

17407_s_at

17484_at

17520_s_at

17555_s_at

17572_s_at

17722_at

17752_at

17840_s_at

17843_s_at

17860_at

17929_s_at

17936_s_at

17962_at

18052_s_at

18069_at

18122_at

18199_at

18259_s_at

18280_at

18289_at

18314_i_at

18318_at

18325_at

18482_s_at

18590_at

18594_at

18595_at

18596_at

18629_s_at

18661_at

18668_at

18699_i_at

18722_s_at

18936_at

18953_at

18955_at

18972_at

19008_s_at

19152_at

19186_s_at

19214_at

19368_at

19379_at

19380_s_at

19421_at

19545_at

19614_at

19638_at

19640_at

19646_s_at

19656_s_at

19701_s_at

19843_at

19944_at

19982_at

19987_at

19991_at

20042_s_at

20060_at

20118_at

20144_at

20149_at

20179_at

20194_at

20245_s_at

20390_s_at

20437_at

20463_s_at

20491_at

20641_at

20658_s_at

[0229]

26

TABLE 26

2X DOWN IN COLD, MANNITOL & SALT, ONLY

12078_at
15189_s_at
17869_at
20015_at

12115_at
15357_at
17888_at
20040_at

12118_at
15364_at
17930_s_at
20089_at

12150_at
15403_s_at
17932_s_at
20190_at

12271_s_at
15476_at
17957_at
20219_at

12276_at
15483_s_at
17963_at
20263_at

12338_at
15522_i_at
17971_s_at
20301_s_at

12400_at
15531_i_at
17975_at
20308_s_at

12430_at
15594_s_at
18016_r_at
20338_at

12538_at
15702_s_at
18140_at
20345_at

12622_at
15778_at
18224_s_at
20395_at

12630_at
15839_at
18225_at
20442_i_at

12792_s_at
15842_at
18228_at
20537_at

12805_s_at
15859_at
18235_at
20573_at

12883_s_at
15872_at
18265_at
20636_at

12909_s_at
15880_at
18270_at
20638_at

12932_s_at
15886_at
18296_at
20698_s_at

12968_at
15906_s_at
18298_at

13159_at
15957_at
18471_at

13217_s_at
15985_at
18564_at

13279_s_at
16045_s_at
18637_at

13282_s_at
16061_s_at
18742_f_at

13432_at
16173_s_at
18761_at

13511_at
16298_at
18833_at

13546_at
16351_at
18875_s_at

13547_s_at
16412_s_at
18894_at

13587_at
16438_at
18946_at

13610_s_at
16493_at
19123_at

13640_at
16534_s_at
19216_at

13725_at
16539_s_at
19243_at

13771_at
16615_s_at
19267_s_at

13916_at
16692_at
19288_at

14028_at
16789_at
19398_at

14039_at
16818_s_at
19424_at

14046_at
16971_s_at
19430_at

14049_at
17018_s_at
19450_at

14077_at
17029_s_at
19457_at

14170_at
17089_s_at
19467_at

14227_at
17228_at
19516_at

14248_at
17338_at
19564_at

14381_at
17387_s_at
19577_at

14384_at
17413_s_at
19593_at

14487_at
17416_at
19602_at

14597_at
17425_s_at
19618_at

14705_i_at
17440_i_at
19670_at

14709_at
17473_at
19696_at

14779_at
17533_s_at
19722_s_at

14947_at
17549_s_at
19749_at

14950_at
17654_at
19755_at

14998_at
17693_at
19815_at

15045_at
17697_at
19926_at

15109_s_at
17755_at
19968_at

15124_s_at
17832_s_at
19977_at

[0230]

27

TABLE 27

ROOT SPECIFIC (COLD, SALINE & OSMOTIC STRESSES)

11997_at
14069_at
16052_at
18327_s_at

12004_at
14072_at
16053_i_at
18597_at

12051_at
14073_at
16105_s_at
18607_s_at

12072_at
14097_at
16161_s_at
18636_at

12150_at
14139_at
16165_s_at
18663_s_at

12151_at
14235_at
16298_at
18782_at

12166_i_at
14250_r_at
16334_s_at
18885_at

12219_at
14578_s_at
16422_at
18888_at

12315_at
14582_at
16427_at
18942_at

12332_s_at
14640_s_at
16440_s_at
18955_at

12374_i_at
14643_s_at
16442_s_at
19060_at

12482_S_at
14644_s_at
16468_at
19108_at

12515_at
14658_s_at
16488_at
19135_at

12522_at
14659_s_at
16511_at
19137_at

12538_at
14711_s_at
16529_at
19195_at

12571_s_at
14900_at
16553 f_at
19263_at

12574_at
14924_at
16568_s_at
19376_at

12609_at
14990_at
16914_s_at
19406_at

12678_i_at
15018_at
16965_s_at
19432_s_at

12698_at
15022_at
16981_s_at
19835_at

12749_at
15107_s_at
16989_at
19836_at

12760_g_at
15116_f_at
17033_s_at
19840_s_at

12765_at
15120_s_at
17066_s_at
19841_at

12768_at
15124_s_at
17085_s_at
19843_at

12769_at
15131_s_at
17252_at
19926_at

12772_at
15132_s_at
17376_at
19972_at

12777_i_at
15137_s_at
17378_at
19977_at

12958_at
15184_s_at
17388_at
19991_at

12989_s_at
15188_s_at
17415_at
20034_i_at

13015_s_at
15208_s_at
17429_s_at
20042_s_at

13134_s_at
15252_g_at
17463_at
20189_at

13146_s_at
15343_at
17485_s_at
20194_at

13172_s_at
15389_at
17490_s_at
20200_at

13178_at
15392_at
17567_at
20214_i_at

13179_at
15448_at
17585_s_at
20239_g_at

13187_i_at
15503_at
17595_s_at
20262_at

13211_s_at
15531_i_at
17840_s_at
20269_at

13239_s_at
15594_s_at
17860_at
20294_at

13273_s_at
15609_s_at
17880_s_at
20312_s_at

13297_s_at
15623_f_at
17894_at
20382_s_at

13549_at
15639_s_at
17896_at
20396_at

13604_at
15670_s_at
17899_at
20432_at

13629_s_at
15680_s_at
17911_at
20444_at

13706_s_at
15859_at
17935_at
20446_s_at

13714_at
15900_at
17961_at
20480_s_at

13751_at
15923_at
18024_s_at
20586_i_at

13895_at
15962_s_at
18122_at
20612_s_at

13933_at
15964_s_at
18222_at
20672_at

13967_at
15965_at
18224_s_at
20686_at

13985_s_at
15975_s_at
18252_at
20689_s_at

14028_at
15985_at
18255_at

14030_at
16001_at
18269_s_at

14058_at
16048_at
18270_at

[0231]

28

TABLE 28

2X LEAF SPECIFIC (COLD, SALINE & OSMOTIC STRESSES)

12169_i_at
16136_s_at

12186_at
16172_s_at

12187_at
16316_at

12211_at
16385_s_at

12212_at
16455_at

12214_g_at
16485_s_at

12270_at
16512_s_at

12645_at
16547_s_at

12754_g_at
16548_s_at

12774_at
16629_s_at

12793_at
16673_at

12796_s_at
16899_at

12910_s_at
17010_s_at

12916_s_at
17018_s_at

12953_at
17054_s_at

13090_at
17095_s_at

13124_at
17097_s_at

13335_at
17273_at

13550_at
17394_s_at

13567_at
17420_at

13568_at
17449_s_at

13596_at
17600_s_at

13614_at
17843_s_at

13678_s_at
17913_s_at

13719_at
17966_at

14014_at
18003_at

14096_at
18081_at

14118_i_at
18560_at

14369_at
18588_at

14478_at
18626_at

14513_s_at
18644_at

14540_at
18666_s_at

14596_at
18742_f_at

14733_s_at
18977_at

14986_at
18994_at

15045_at
19227_at

15097_s_at
19373_at

15098_s_at
19834_at

15145_s_at
19867_at

15153_s_at
19998_at

15154_s_at
20062_at

15182_s_at
20199_at

15203_s_at
20256_s_at

15372_at
20284_at

15521_s_at
20437_at

15581_s_at
20442_i_at

15621_f_at
20450_at

15642_s_at
20468_at

15776_at
20547_at

15910_at
20635_s_at

16017_at

16046_s_at

16115_s_at

[0232]

29

TABLE 29

2X TRANSCRIPTION (COLD, SALINE & OSMOTIC STRESSES)

12068_at
15665_s_at
19836_at

12166_i_at
15679_s_at
19860_at

12374_i_at
15720_at
19866_at

12392_at15871_s_at
19898_at

12431_at
16072_s_at
20262_at

12450_s_at
16073_f_at
20335_s_at

12503_at
16105_s_at
20362_at

12536_s_at
16111_f_at
20424_at

12540_s_at
16127_s_at
20437_at

12541_at
16534_s_at
20456_at

12587_at
16582_s_at
20515_s_at

12594_at
16589_s_at
20635_s_at

12595_at
16747_at

12704_f_at
17019_s_at

12705_f_at
17129_s_at

12709_f_at
17160_at

12712_f_at
17520_s_at

12719_f_at
17538_s_at

12724_f_at
17555_s_at

12725_r_at
17609_at

12726_f_at
17896_at

12734_f_at
17971_s_at

12736_f_at
17975_at

12737_f_at
17978_s_at

12812_at
18121_s_at

12949_at
18167_s_at

12951_at
18197_at

12966_s_at
18222_at

13023_at
18318_at

13034_s_at
18576_s_at

13087_at
18629_s_at

13270_at
18738_f_at

13273_s_at
18742_f_at

13432_at
18744_f_at

13555_at
18745_f_at

13688_s_at
18747_f_at

13714_at
18750_f_at

13965_s_at
18751_f_at

13987_s_at
18789_at

14003_at
18834_at

14144_at
18942_at

14178_at
19083_at

14223_at
19202_at

14235_at
19209_s_at

14303_s_at
19232_s_at

14393_at
19315_at

14553_at
19489_s_at

14781_at
19611_s_at

15046_s_at
19646_s_at

15053_s_at
19707_s_at

15214_s_at
19722_s_at

15510_r_at
19744_at

15638_s_at
19755_at

[0233]

30

TABLE 30

2X PHOSPHATES (COLD, SALINE & OSMOTIC STRESSES)

12470_at

12556_at

13128_at

13135_s_at

13180_s_at

13192_s_at

13193_s_at

13587_at

13995_at

14335_at

15073_at

15171_s_at

15240_at

15586_s_at

15641_s_at

15651_f_at

15990_at

16232_S_at

16576 f at

16753_at

17423_s_at

17525_s_at

17537_s_at

17929_s_at

17954_s_at

18012_s_at

18308_i_at

18616_at

18847_at

18936_at

18980_at

19243_at

19263_at

19638_at

19883_at

19932_at

20333_at

20393_at

20570_at

[0234]

31

TABLE 31

2X KINASES (COLD, SALINE & OSMOTIC STRESSES)

12253_g_at
16059_s_at
20144_at

12270_at
16087_s_at
20219_at

12271_s_at
16088_f_at
20223_at

12276_at
16125_s_at
20232_s_at

12278_at
16137_s_at
20235_i_at

12284_at
16140_s_at
20282_s_at

12300_at
16143_s_at
20298_at

12307_at
16144_s_at
20396_at

12353_at
16160_f_at
20439_at

12357_s_at
16171_s_at
20462_at

12390_at
16357_at

12394_at
16412_s_at

12395_s_at
16568_s_at

12408_at
16570_s_at

12452_at
16571_s_at

12477_at
16584_s_at

12490_at
16651_s_at

12497_at
16652_s_at

12532_at
16672_at

12697_at
16818_s_at

12901_s_at
16840_at

12902_at
17068_s_at

12958_at
17122_s_at

12959_at
17252_at

13068_at
17323_at

13246_at
17475_at

13324_at
17752_at

13332_at
17921_s_at

13362_s_at
17933_at

13370_at
17935_at

13550_at
18013_r_at

14030_at
18046_s_at

14048_at
18122_at

14194_at
18176_at

14196_at
18316_at

14217_at
18455_at

14459_at
18459_at

14603_at
18482_s_at

14637_s_at
18543_at

14686_s_at
18706_s_at

15005_s_at
18782_at

15175_s_at
18924_at

15270_at
19117_s_at

15475_s_at
19437_s_at

15497_s_at
19442_at

15577_s_at
19458_at

15616_s_at
19464_at

15633_s_at
19469_at

15634_s_at
19562_at

15668_s_at
19655_at

15680_s_at
19749_at

15798_at
19854_at

16034_at
19904_at

[0235]

Number	Date	Country
60227866	Aug 2000	US
60264647	Jan 2001	US
60300111	Jun 2001	US

Stress-regulated genes of plants, transgenic plants containing same, and methods of use

Information

Publication Number

Date Filed

Date Published

Inventors

CPC

US Classifications

International Classifications

Abstract

Description

Claims

Parent Case Info

Provisional Applications (3)