Stress-regulated genes of plants, transgenic plants containing same, and methods of use

Three CD-R compact discs, labeled “Copy 1”, “Copy 2”, and “CRF” and having the files listed below, are submitted herewith and are incorporated herein by reference. Copy 1 and Copy 2 each contain two text documents: 1) a file named SCRIP1300-3_SEQUENCE_LISTING, which contains the Sequence Listing, was created on Aug. 20, 2001 (and recorded on the CD-R on Aug. 21, 2001), and is 9,972 KB in size; and 2) a file named SCRIP1300-3_Table_—32, which contains Table 32, was created on Aug. 20, 2001 (and recorded on the CD-R on Aug. 21, 2001), and is 1,251 KB in size. The CRF contains a single file named SCRIP1300-3_SEQUENCE_LISTING, which contains the Sequence Listing, was created on Aug. 20, 2001 (and recorded on the CD-R on Aug. 21, 2001), is 9,972 KB in size, and is identical to the files having the same name on Copy 1 and Copy 2.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates generally to plant genes, the expression of which are regulated in response to stress, and more specifically to the gene regulatory elements involved in a stress-induced response in plants, to uses of the coding sequences and regulatory elements of such plant stress-regulated genes, and to transgenic plants genetically modified to express such a coding sequence or to express a heterologous polynucleotide from such a regulatory element.

2. Background Information

Microarray technology is a powerful tool that can be used to identify the presence and level of expression of a large number of polynucleotides in a single assay. A microarray is formed by linking a large number of discrete polynucleotide sequences, for example, a population of polynucleotides representative of a genome of an organism, to a solid support such as a microchip, glass slide, or the like, in a defined pattern. By contacting the microarray with a nucleic acid sample obtained from a cell of interest, and detecting those polynucleotides expressed in the cell can hybridize specifically to complementary sequences on the chip, the pattern formed by the hybridizing polynucleotides allows the identification of clusters of genes that are expressed in the cell. Furthermore, where each polynucleotide linked to the solid support is known, the identity of the hybridizing sequences from the nucleic acid sample can be identified.

A strength of microarray technology is that it allows the identification of differential gene expression simply by comparing patterns of hybridization. For example, by comparing the hybridization pattern of nucleic acid molecules obtained from cells of an individual suffering from a disease with the nucleic acids obtained from the corresponding cells of a healthy individual, genes that are differentially expressed can be identified. The identification of such differentially expressed genes provides a means to identify new genes, and can provide insight as to the etiology of a disease.

Microarray technology has been widely used to identify patterns of gene expression associated with particular stages of development or of disease conditions in animal model systems, and is being applied to the identification of specific patterns of gene expression in humans. The recent availability of information for the genomes of plants provides a means to adapt microarray technology to the study of plant gene expression.

Plants and plant products provide the primary sustenance, either directly or indirectly, for all animal life, including humans. For the majority of the world's human population and for many animals, plants and plant products provide the sole source of nutrition. As the world population increases, the best hope to prevent widespread famine is to increase the quantity and improve the quality of food crops, and to make the crops available to the regions of the world most in need of food.

Throughout history, a continual effort has been made to increase the yield and nutritious value of food crops. For centuries, plants having desirable characteristics such as greater resistance to drought conditions or increased size of fruit were crossbred and progeny plants exhibiting the desired characteristics were selected and used to produce seed or cuttings for propagation. Using such classical genetic methods, plants having, for example, greater disease resistance, increased yield, and better flavor have been obtained. The identification of plant genes involved in conferring a selective advantage on the plant to an environmental challenge would facilitate the generation and yield of plants, thereby increasing the available food supply to an increasing world population. The involvement of these genes in a single organism to responses to multiple stress conditions, however, remains unknown. Thus, a need exists to identify plant genes and polynucleotides that are involved in modulating the response of a plant to changing environmental conditions. The present invention satisfies this need and provides additional advantages.

SUMMARY OF THE INVENTION

The present invention relates to clusters of genes that are regulated in response to a stress condition in plants. Such clusters include, for example, plant polynucleotides whose expression is altered in response to two or more different stress conditions; and plant polynucleotides the expression of which are altered in response to one stress condition, but not to others. The identification of such clusters, using microarray technology, has allowed the identification of plant stress-regulated genes in Arabidopsis thaliana (see Tables 1 and 2); and homologs and orthologs thereof in other plant species (see Table 32). Thus, the invention provides isolated polynucleotide portions of Arabidopsis plant stress-regulated genes, and homologs and orthologs thereof; variants of such sequences, and polynucleotides encoding substantially similar plant stress-regulated polypeptides expressed therefrom. Such sequences include, for example, sequences encoding transcription factors; enzymes, including kinases; and structural proteins, including channel proteins (see Tables 29–31). Accordingly, the present invention also relates to an isolated polynucleotide comprising all or a portion of a plant stress-regulated gene, and to polynucleotide portions thereof, including a coding region (open reading frame), which encodes all or a portion of a stress-regulated polypeptide, for example, as set forth in SEQ ID NOS:1–2703; and a regulatory element involved in regulating the response of the plant to a stress condition such exposure to an abnormal level of salt, osmotic pressure, temperature or any combination thereof, for example, as set forth in SEQ ID NOS:2704–5379.

The present invention also relates to a recombinant polynucleotide, which contains a nucleotide sequence of a plant stress-regulated gene or functional portion thereof operatively linked to a heterologous nucleotide sequence. In one embodiment, the recombinant polynucleotide comprises a plant stress-regulated gene regulatory element operatively linked to a heterologous nucleotide sequence, which is not regulated by the regulatory element in a naturally occurring plant. The heterologous nucleotide sequence, when expressed from the regulatory element, can confer a desirable phenotype to a plant cell containing the recombinant polynucleotide. In another embodiment, the recombinant polynucleotide comprises a coding region, or portion thereof, of a plant stress-regulated gene operatively linked to a heterologous promoter. The heterologous promoter provides a means to express an encoded stress-regulated polypeptide constitutively, or in a tissue-specific or phase-specific manner.

Accordingly, in one aspect, the present invention provides an isolated polynucleotide comprising a nucleotide sequence of a plant gene that hybridizes under stringent conditions, preferably high stringency conditions, to any one of SEQ ID NOS:1–5379 (see Tables 1 and 2), including to a coding region (SEQ ID NOS:1–2703) or a regulatory region, which can alter transcription of an operatively linked nucleic acid sequence in response to an abiotic stress (SEQ ID NOS:2704–5379; see Table 2), or to a complement thereof. Additional aspects provide sequences that hybridize under stringent conditions, preferably high stringency conditions, to the complements of SEQ ID NO 1–1261 (cold responsive genes; Tables 3–6), SEQ ID NOS:2227–2427 (saline responsive genes; Tables 7–10), SEQ ID NOS:2428–2585 (osmotic responsive genes; Tables 11–14), SEQ ID NOS:1699–1969 (cold and osmotic responsive genes; Tables 15–17), SEQ ID NOS:1970–2226 (cold and saline responsive genes; Tables 18–20), SEQ ID NOS:2586–2703 (osmotic and saline responsive genes; Tables 21–23), and SEQ ID NOS:1262–1698 (cold, osmotic and saline responsive genes; Tables 24–26), and which can comprise regulatory regions that can alter transcription in response to cold stress, osmotic stress, saline stress, or combinations thereof (SEQ ID NOS:2704–5379; see Table 2). Also provided are nucleotide sequences complementary thereto, and expression cassettes, plants and seeds comprising any of the above isolated sequences.

In another aspect, the present invention provides an isolated polynucleotide comprising a plant nucleotide sequence that hybridizes under stringent conditions, preferably high stringency conditions, to the complement of any one of SEQ ID NOS:1–2703 (Table 1), including to a coding region thereof (SEQ ID NOS:2704–5379), wherein expression of said coding region is altered in response to an abiotic stress. Additional aspects provide sequences that hybridize under high stringency conditions to the complements of SEQ ID NO 1–1261 (cold responsive genes; Tables 3–6), SEQ ID NOS:2227–2427 (saline responsive genes; Tables 7–10), SEQ ID NOS:2428–2585 (osmotic responsive genes; Tables 11–14), SEQ ID NOS:1699–1969 (cold and osmotic responsive genes; Tables 15–17), SEQ ID NOS:1970–2226 (cold and saline responsive genes; Tables 18–20), SEQ ID NOS:2586–2703 (osmotic and saline responsive genes; Tables 21–23), and SEQ ID NOS:1262–1698 (cold, osmotic and saline responsive genes; Tables 24–26), and which can comprise a coding region whose transcription is altered in response to cold stress, osmotic stress, saline stress, or a combination thereof. Also provided are nucleotide sequences complementary thereto, and expression cassettes, plants and seeds comprising any of the above sequences.

The invention further relates to a method of producing a transgenic plant, which comprises at least one plant cell that exhibits altered responsiveness to a stress condition. In one embodiment, the method can be performed by introducing a polynucleotide portion of plant stress-regulated gene into a plant cell genome, whereby the polynucleotide portion of the plant stress-regulated gene modulates a response of the plant cell to a stress condition.

The polynucleotide portion of the plant stress-regulated gene can encode a stress-regulated polypeptide or functional peptide portion thereof (see SEQ ID NOS:1–2703), wherein expression of the stress-regulated polypeptide or functional peptide portion thereof either increases the stress tolerance of the transgenic plant, or decreases the stress tolerance of the transgenic plant. The polynucleotide portion of the plant stress-regulated gene encoding the stress-regulated polypeptide or functional peptide portion thereof can be operatively linked to a heterologous promoter. The polynucleotide portion of the plant stress-regulated gene also can comprise a stress-regulated gene regulatory element (see SEQ ID NOS:2704–5379). The stress-regulated gene regulatory element can integrate into the plant cell genome in a site-specific manner, whereupon it can be operatively linked to a heterologous nucleotide sequence, which can be expressed in response to a stress condition specific for the regulatory element; or can be a mutant regulatory element, which is not responsive to the stress condition, whereby upon integrating into the plant cell genome, the mutant regulatory element disrupts an endogenous stress-regulated regulatory element of a plant stress-regulated gene, thereby altering the responsiveness of the plant stress-regulated gene to the stress condition.

In one aspect, the invention provides a method for producing a transgenic plant by introducing into at least one plant cell a recombinant nucleic acid construct comprising i) all or a portion of any one of SEQ ID NOS:1–5379; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to all or a portion of the complement of any one of SEQ ID NOS:1–2703; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to abiotic stress, and that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2704–5379; iv) a polynucleotide having at least 90% sequence identity with any one of SEQ ID NO:1–5379; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a nucleotide sequence that alters transcription of an operatively linked coding region in response to abiotic stress; and regenerating a plant from the at least one plant cell.

Another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:1–1261 or 2704–3955; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:1–1261; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to cold stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2704–3955; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:1–1261 or 2704–3955; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv) wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to cold stress; and regenerating a plant from the at least one plant cell.

In another aspect, the invention provides a method for producing a transgenic plant by introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:2428–2585 or 5108–5263; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2428–2585; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to osmotic stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:5108–5263; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:2428–2585 or 5108–5263; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to osmotic stress; and regenerating a plant from the at least one plant cell.

Still another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:2227–2427 or 4910–5107; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2227–2427; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to saline stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2227–2427; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:4910–5107; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv) wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to saline stress; and regenerating a plant from the at least one plant cell.

Yet another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:1699–1969 or 4389–4654; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:1699–1969; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to a combination of cold and osmotic stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:4389–4654; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:1699–1969 or 4389–4654; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to a combination of cold and osmotic stress; and regenerating a plant from the at least one plant cell.

Yet another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:1970–2226 or 4655–4909; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:1970–2226; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to a combination of cold and saline stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:4655–4909; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:1970–2226 or 4655–4909; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to a combination of cold and saline stress; and regenerating a plant from the at least one plant cell.

A further aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:2586–2703 or 5264–5379; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:2586–2703; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to a combination of osmotic and saline stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS: 5264–5379; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:2586–2703 or 5264–5379; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv), wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to a combination of osmotic and saline stress; and regenerating a plant from the at least one plant cell.

Another aspect provides a method for producing a transgenic plant comprising introducing into at least one plant cell a recombinant nucleic acid construct comprising i) any one of SEQ ID NOS:1262–1698 or 3956–4388; ii) a polynucleotide comprising a coding region that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:1262–1698; iii) a polynucleotide comprising a sequence that alters transcription of an operatively linked coding region in response to a combination of cold, osmotic and saline stress that hybridizes under conditions of high stringency to the complement of any one of SEQ ID NOS:3956–4388; iv) a polynucleotide that has at least 90% sequence identity with any one of SEQ ID NOS:1262–1698 or 3956–4388; v) a fragment of any one of the sequences of iv), wherein the fragment comprises a coding region; or vi) a fragment of any one of the sequences of iv) wherein the fragment comprises a sequence or region that alters transcription of an operatively linked coding region in response to a combination of cold, osmotic and saline stress; and regenerating a plant from the at least one plant cell. Further aspects include plants and uniform populations of plants made by the above methods as well as seeds and progeny from such plants.

In another embodiment, a transgene introduced into a plant cell according to a method of the invention can encode a polypeptide that regulates expression from an endogenous plant stress-regulated gene. Such a polypeptide can be, for example, a recombinantly produced polypeptide comprising a zinc finger domain, which is specific for the regulatory element, and an effector domain, which can be a repressor domain or an activator domain. The polynucleotide encoding the recombinant polypeptide can be operatively linked to and expressed from a constitutively active, inducible or tissue specific or phase specific regulatory element. Expression of the recombinant polypeptide from a plant stress-regulated promoter as disclosed herein can be particularly advantageous in that the polypeptide can be coordinately expressed with the endogenous plant stress-regulated genes upon exposure to a stress condition. The invention also provides transgenic plants produced by a method as disclosed, as well as to a plant cell obtained from such transgenic plant, wherein said plant cell exhibits altered responsiveness to the stress condition; a seed produced by the transgenic plant; and a cDNA or genomic DNA library prepared from the transgenic plant, or from a plant cell from said transgenic plant, wherein said plant cell exhibits altered responsiveness to the stress condition.

In one aspect, the invention provides an isolated nucleic acid molecule comprising a nucleotide sequence substantially similar to a sequence of any one of SEQ ID NOS:2704–5379, which can alter transcription of an operatively linked polynucleotide in a plant cell in response to an abiotic stress. Additional aspects of the invention provide isolated polynucleotides, including, for example, sequences substantially similar to any of SEQ ID NOS:2704–3955, which can alter transcription of an operatively linked polynucleotide in response to a cold stress; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:5108–5263, which can alter transcription of an operatively linked polynucleotide in response to an osmotic stress; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:4910–5107, which can alter transcription of an operatively linked polynucleotide in response to a saline stress; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:4389–4654, which can alter transcription of an operatively linked polynucleotide in response to a combination of cold and osmotic stresses; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:4655–4909, which can alter transcription of an operatively linked polynucleotide in response to a combination of cold and saline stresses; isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:5264–5379, which can alter transcription of an operatively linked polynucleotide in response to a combination of osmotic and saline stresses; and isolated polynucleotides substantially similar to a sequence of any of SEQ ID NOS:3956–4388, which can alter transcription of an operatively linked polynucleotide in response to a combination of cold, osmotic and saline stresses.

Related aspects of the invention provide an isolated nucleotide sequences that can alter transcription of an operatively linked polynucleotide in response to an abiotic stress, and that hybridize under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:2704–5379. Additional aspects provide an isolated nucleotide sequence that can alter transcription of an operatively linked polynucleotide in response to cold stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:2704–3955; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to osmotic stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:5108–5263; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to saline stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:4910–5107; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to a combination of cold and osmotic stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:4389–4654; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to a combination of cold and saline stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:4655–4909; a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to an combination of osmotic and saline stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:5264–5379; and a nucleotide sequence that alters transcription of an operatively linked polynucleotide in response to a combination of cold, osmotic and saline stress, and that hybridizes under stringent conditions, preferably highly stringent conditions, to the complement of any one of SEQ ID NOS:3956–4388.

Further aspects provide an expression cassette comprising as operatively linked components any of the above isolated nucleic acid sequences that alter transcription, a coding region, and a termination sequence. Also provided are host cells and seeds comprising such expression cassettes, plants containing such host cells and seeds and progeny of plants containing said host cells. In related aspects, the coding region of the expression cassettes comprise sequences encoding marker proteins and sequences involved in gene silencing such as antisense sequences, double stranded RNAi sequences, a triplexing agent, and sequences comprising dominant negative mutations. In additional related aspects, the coding regions comprise sequences encoding polypeptides that alter the response of a plant to an abiotic stress.

The present invention also relates to a method of modulating the responsiveness of a plant cell to a stress condition. Such a method can be performed, for example, by introducing a polynucleotide portion of a plant stress-regulated genes described herein into the plant cell, thereby modulating the responsiveness of the plant cell to a stress condition. Such a method can result in the responsiveness of the plant cell being increased upon exposure to the stress condition, which, in turn, can result in increased or decreased tolerance of the plant cell to a stress condition; or can result in the responsiveness of the plant cell to the stress condition being decreased, which, in turn, can result in increased or decreased tolerance of the plant cell to a stress condition. In one embodiment, the polynucleotide portion of the plant stress-regulated gene can integrate into the genome of the plant cell, thereby modulating the responsiveness of the plant cell to the stress condition. In another embodiment, the polynucleotide portion of the plant stress-regulated gene encodes a stress-regulated polypeptide or functional peptide portion thereof, and can be operatively linked to a heterologous promoter. The polynucleotide portion of the plant stress-regulated gene also can contain a mutation, whereby upon integrating into the plant cell genome, the polynucleotide disrupts (knocks-out) an endogenous plant stress-regulated sequence, thereby modulating the responsiveness of the plant cell to the stress condition. Depending on whether the knocked-out gene encodes an adaptive or a maladaptive stress-regulated polypeptide, the responsiveness of the plant will be modulated accordingly.

The present invention further relates to a method of modulating the activity of a biological pathway in a plant cell, wherein the pathway involves a stress-regulated polypeptide or a non-protein regulatory molecule. Such a method can be performed by introducing a polynucleotide portion of a plant stress-regulated gene, or a polynucleotide derived therefrom, for example a ribozyme derived from a nucleotide sequence as set forth in any of SEQ ID NOS:1–2703, into the plant cell, thereby modulating the activity of the biological pathway. The method can be performed with respect to a pathway involving any of the stress-regulated polypeptides as disclosed herein or encoded by the polynucleotides disclosed herein, as well as using homologs or orthologs thereof. In one embodiment, the method is performed by introducing a polynucleotide portion of a plant stress-regulated gene into the plant cell, wherein the plant stress-regulated gene comprises a nucleotide sequence as set forth in any of SEQ ID NOS:1–155, 157–228, 230–232, 234–557, 559–572, 574–605, 607–634, 636–786, 788–812, 814–1262, 1264–1386, 1387–1390, 1392–1404, 1406–1444, 1446–1483, 1485–1588, 1590–1608, 1610–1633, 1634–1725, 1727–1865, 1867–1917, 1919–1927, 1929–2855, 2857–2928, 2930–2932, 2934–3256, 3258–3271, 3273–3304, 3306–3323, 3325–3333, 3335–3485, 3487–3511, 3313–3956, 3958–4078, 4080–4097, 4099–4136, 4138–4175, 4177–4279, 4281–4299, 4301–4324, 4326–4414, 4416–4552, 4554–4602, and 4604–5379, thereby modulating the activity of the biological pathway.

The present invention also relates to a method of identifying a polynucleotide that modulates a stress response in a plant cell. In one embodiment the method comprises determining gene expression in a plant exposed to at least one stress to produce an expression profile and identifying sequences whose expression is altered at least two fold compared to plants not exposed to the stress. Such an expression profile can be obtained, for example, by contacting an array of probes representative of a plant cell genome with nucleic acid molecules expressed in a plant cell exposed to the stress; and detecting one or more nucleic acid molecules expressed at a level different from a level of expression in the absence of the stress. The method can further comprise introducing the differentially expressed nucleic acid molecule into a plant cell; and detecting a modulated response of the genetically modified plant cell to a stress, thereby identifying a polynucleotide that modulates a stress response in a plant cell. The stress can be any stress, for example, an abiotic stress such as exposure to an abnormal level of cold, osmotic pressure, and salinity. The contacting is under conditions that allow for selective hybridization of a nucleic acid molecule with probe having sufficient complementarity, for example, under stringent hybridization conditions. Expression of the nucleic acid molecule can increase or decrease the tolerance of the plant cell to the stress, and the nucleic acid molecule can be expressed at a level that is less than or greater than the level of expression in the absence of the stress.

In still another embodiment, the polynucleotide portion of the plant stress-regulated gene can comprise a stress-regulated regulatory element, which can be operatively linked to a heterologous nucleotide sequence, the expression of which can modulate the responsiveness of the plant cell to a stress condition. Such a heterologous nucleotide sequence can encode, for example, a stress-inducible transcription factor such as DREB1A. The heterologous nucleotide sequence also can encode a polynucleotide that is specific for a plant stress-regulated gene, for example, an antisense molecule, an RNAi molecule, a ribozyme, and a triplexing agent, any of which, upon expression in the plant cell, reduces or inhibits expression of a stress-regulated polypeptide encoded by the gene, thereby modulating the responsiveness of the plant cell to a stress condition, for example, an abnormal level of cold, osmotic pressure, and salinity. In another aspect, the method can include introducing a polynucleotide portion of a plant stress-regulated gene into the plant cell, wherein the plant stress-regulated gene includes a nucleotide sequence of a polynucleotide as set forth in any of SEQ ID NOS:1–155, 157–228, 230–232, 234–557, 559–572, 574–605, 607–634, 636–786, 788–812, 814–1262, 1264–1386, 1387–1390, 1392–1404, 1406–1444, 1446–1483, 1485–1588, 1590–1608, 1610–1633, 1634–1725, 1727–1865, 1867–1917, 1919–1927, 1929–2855, 2857–2928, 2930–2932, 2934–3256, 3258–3271, 3273–3304, 3306–3323, 3325–3333, 3335–3485, 3487–3511, 3313–3956, 3958–4078, 4080–4097, 4099–4136, 4138–4175, 4177–4279, 4281–4299, 4301–4324, 4326–4414, 4416–4552, 4554–4602, and 4604–5379, thereby modulating the responsiveness of the plant cell to a stress condition. The invention also relates to a plant cell obtained by any of the methods of modulating the responsiveness of a plant to a stress condition or combination of stress conditions, and to a plant comprising such a plant cell.

The present invention further relates to a method of selecting a plant having an altered resistance to an abiotic stress condition or a combination of abiotic stress conditions, such a method being useful for marker-assisted breeding. Such a method can be performed, for example, by contacting nucleic acid molecules representative of expressed polynucleotides in a plant cell of a plant to be examined for having an altered resistance to an abiotic stress with a nucleic acid probes that selectively hybridizes under stringent conditions to a plant stress-regulated gene comprising a nucleotide sequence as set forth in any of SEQ ID NO:1–5379; detecting a level of selective hybridization of the nucleic acid probes to a nucleic acid molecule representative of an expressed polynucleotide in the plant cell, wherein the level of selective hybridization corresponds to the level of the expressed polynucleotide in the plant cell, which is indicative of resistance of the plant to an abiotic stress; and selecting a plant having a level of expression of a polynucleotide indicative of altered resistance to an abiotic stress condition. For example, the abiotic stress condition can be cold stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1–1261 and 2704–3955, for example, at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1–155, 157–228, 230–232, 234–557, 559–572, 574–605, 607–634, 636–786, 788–812, 814–1261, 2704–2855, 2857–2928, 2930–2932, 2934–3256, 3258–3271, 3273–3304, 3306–3323, 3325–3333, 3335–3485, 3487–3511, and 3313–3955; or the abiotic stress condition can be saline stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2226–2427 and 4910–5107; or the abiotic stress condition can be osmotic stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2428–2585 and 5108–5263. In addition, a combination of abiotic stress conditions can be a combination of cold stress and osmotic stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1669–1969 and 4389–4654, for example, at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1699–1725, 1727–1865, 1867–1917, 1919–1927, 1929–1969, 4389–4414, 4416–4552, 4554–4602, 4604–4612, and 4613–4654; or the combination of abiotic stress conditions can be a combination of cold stress and saline stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS: 1970–2226 and 4655–4909; or the combination of abiotic stress conditions can be a combination of osmotic stress and saline stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2586–2703 and 5264–5379; or the combination of abiotic stress conditions can be a combination of cold stress, osmotic stress and saline stress, and the nucleic acid probe can include at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1262–1698 and 3956–4388, for example, at least about 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1262, 1264–1386, 1387–1390, 1392–1404, 1406–1444, 1446–1483, 1485–1588, 1590–1608, 1610–1633, 1634–1698, 3956, 3958–4078, 4080–4097, 4099–4136, 4138–4175, 4177–4279, 4281–4299, 4301–4324, and 4326–4388.

The present invention also relates to a method of expressing a heterologous nucleotide sequence in a plant cell. Such a method can be performed, for example, by introducing into the plant cell a plant stress-regulated regulatory element operatively linked to the heterologous nucleotide sequence, whereby, upon exposure of the plant cell to a stress condition, the heterologous nucleotide sequence is expressed in the plant cell. In one embodiment, the stress-regulated gene regulatory element is any of the sequences described herein that are capable of altering transcription of an operatively linked sequence in response to an abiotic stress, for example, SEQ ID NOS:2704–5379. In another embodiment, stress-regulated gene regulatory element comprises a nucleotide sequence as set forth in any of SEQ ID NOS:2704–2855, 2857–2928, 2930–2932, 2934–3256, 3258–3271, 3273–3304, 3306–3323, 3325–3333, 3335–3485, 3487–3511, 3313–3956, 3958–4078, 4080–4097, 4099–4136, 4138–4175, 4177–4279, 4281–4299, 4301–4324, 4326–4414, 4416–4552, 4554–4602, and 4604–5379, whereby, upon exposure of the plant cell to stress condition, the heterologous nucleotide sequence is expressed in the plant cell. The heterologous nucleotide sequence can encode a selectable marker, a diagnostic marker, or a polypeptide that confers a desirable trait upon the plant cell, for example, a polypeptide that improves the nutritional value, digestibility or ornamental value of the plant cell, or a plant comprising the plant cell.

The present invention additionally relates to a method of identifying a stress condition to which a plant cell was exposed by comparing an expression profile from a test plant suspected of having been exposed to at least one stress condition to an expression profile obtained from a reference plant, preferably of the same species, which has been exposed to the suspected stress condition. Such a method can be performed, for example, by contacting nucleic acid molecules representative of expressed polynucleotides in cells of the test plant with at least one nucleic acid probe under conditions suitable for selective hybridization to a complementary nucleotide sequence, wherein the probe comprises at least 15 nucleotides of a plant stress-regulated gene, wherein the stress-regulated gene does not have a nucleotide sequence of a polynucleotide as set forth in any of SEQ ID NOS:156, 229, 233, 558, 573, 606, 635, 787, 813, 1263, 1386, 1391, 1405, 1445, 1484, 1589, 1609, 1634, 1726, 1866, 1918 or 1928, or a nucleotide sequence complementary thereto, whereby detecting selective hybridization of at least one nucleic acid probe, or detecting a change in a level of selective hybridization as compared to a level of selective hybridization obtained using nucleic acid molecules representative of expressed polynucleotides in cells of a plant known not have been exposed to an abiotic stress, indicates that the test plant has been exposed to an abiotic stress, and whereby an absence of selective hybridization of at least one nucleic acid probe indicates that the test plant has not been exposed to an abiotic stress. For example, the abiotic stress is cold stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1–155, 157–228, 230–232, 234–557, 559–572, 574–605, 607–634, 636–786, 788–812, 814–1261 or a nucleotide sequence complementary thereto; or the abiotic stress can be a saline stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2226–2427 or a nucleotide sequence complementary thereto; or the abiotic stress can be an osmotic stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in two or more of SEQ ID NOS:2428–2585 or a nucleotide sequence complementary thereto.

A method of identifying a stress condition to which a plant cell was exposed also can be performed, for example, by contacting nucleic acid molecules expressed in the test plant cell with an array of probes representative of the plant cell genome; detecting a profile of expressed nucleic acid molecules characteristic of a stress response, and comparing the expression pattern in the test plant to the expression pattern obtained from a reference plant thereby identifying the stress condition to which the plant cell was exposed. The contacting is under conditions that allow for selective hybridization of a nucleic acid molecule with probes having sufficient complementarity, for example, under stringent hybridization conditions. The profile can be characteristic of exposure to a single stress condition, for example, an abnormal level of cold, osmotic pressure, or salinity, or can be characteristic of exposure to more than one stress condition, for example, cold, increased osmotic pressure and increased salinity. In one embodiment, the nucleotide sequence of a gene whose expression is detected is selected from a polynucleotide comprising any of SEQ ID NOS:1–2703. In further embodiments, the nucleotide sequence of a gene that is expressed in response a particular stress or combination of stresses can comprise a polynucleotide expressed in response to cold stress (SEQ ID NOS:1–1261), osmotic stress (SEQ ID NOS:2428–2585), saline (salt) stress (SEQ ID NOS:2227–2427), a combination of cold and osmotic stress (SEQ ID NOS:1699–1969), a combination of saline and osmotic stress (SEQ ID NOS:1970–2226), a combination of osmotic and saline stress (SEQ ID NOS:2586–2703), or a combination of cold, osmotic and saline stress (SEQ ID NOS:1262–1698).

In another embodiment, the method can be used for determining whether a test plant has been exposed to a combination of abiotic stress conditions. Such a method can be performed, for example, by contacting nucleic acid molecules representative of expressed polynucleotides in cells of the test plant with at least one nucleic acid probe under conditions suitable for selective hybridization to a complementary nucleotide sequence, whereby detecting selective hybridization of at least one nucleic acid probe, or detecting a change in a level of selective hybridization as compared to a level of selective hybridization obtained using nucleic acid molecules representative of expressed polynucleotides in cells of a plant known not have been exposed to a combination of stress conditions, indicates that the test plant has been exposed to a combination of abiotic stress conditions, and whereby an absence of selective hybridization of at least one nucleic acid probe indicates that the test plant has not been exposed to a combination of abiotic stress conditions. For example, the combination of abiotic stress conditions can be a combination of a cold stress and an osmotic stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1699–1969, or a nucleotide sequence complementary thereto; or the combination of abiotic stress conditions can be a combination of a cold stress and a saline stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1970–2226, or a nucleotide sequence complementary thereto; or the combination of abiotic stress conditions can be a combination of an osmotic stress and a saline stress, and the probe can included at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:2586–2703, or a nucleotide sequence complementary thereto; or the combination of abiotic stress conditions can be a combination of a cold stress, a saline stress and an osmotic stress, and the probe can include at least 15 nucleotides of a nucleotide sequence as set forth in any of SEQ ID NOS:1262–1698, or a nucleotide sequence complementary thereto.

The present invention also relates to a method for monitoring a population of plants for exposure to a stress condition or combination of stress conditions. Such a method can be performed, for example, by introducing into the population of a plants a sentinel plant, wherein said sentinel plant is a transgenic plant, which contains plant cells containing a stress-regulated regulatory element operatively linked to a polynucleotide encoding a detectable marker; and examining the sentinel plant for expression of the detectable marker, which is indicative of exposure of the population of plants to a stress condition or combination of stress conditions. The stress condition or combination of stress conditions can be any such condition or conditions, particularly an abiotic stress condition or combination of abiotic stress conditions. The detectable marker can be any reporter molecule that is readily or conveniently detectable, particularly a marker that is visibly detectable, for example, a luminescent detectable marker such as luciferin, or a fluorescent detectable marker such as a green fluorescent protein, a yellow fluorescent protein, a cyan fluorescent protein, a red fluorescent protein, or an enhanced or modified form thereof.

The present invention further relates to a transgenic plant, which contains a nucleic acid construct comprising a polynucleotide portion of plant stress-regulated polynucleotide. In one embodiment, the transgenic plant exhibits altered responsiveness to a stress condition as compared to a corresponding reference plant not containing the construct. Such a transgenic plant can contain, for example, a construct that disrupts an endogenous stress-regulated gene in the plant, thereby reducing or inhibiting expression of the gene in response to a stress condition. Such a knock-out can increase or decrease tolerance of the plant to a stress condition. The transgene also can comprise a coding sequence of a plant stress-regulated gene, which can be operatively linked to a heterologous regulatory element such as a constitutively active regulatory element, an regulated regulatory element, a tissues specific or phase specific regulatory element, or the like. In another embodiment, the transgenic plant contains a nucleic acid construct comprising a plant stress-regulated regulatory element, which can be operatively linked to a heterologous nucleotide sequence that can encode a polypeptide. Expression of the heterologous polypeptide can confer a desirable characteristic on the plant, for example, can improve the nutritional or ornamental value of the transgenic plant. In still another embodiment, the transgenic plant contains multiple nucleic acid constructs, which can be multiple copies of the same construct, or can be two or more different constructs.

The present invention also relates to a plant stress-regulated regulatory element, which is obtained from a plant stress-regulated polynucleotide disclosed herein for example any of SEQ ID NOS:2704–5379; a homolog or ortholog thereof The invention also provides a method of identifying an agent, for example a transcription factor, that specifically binds to or activates a plant stress-regulated regulatory element. Such a method can be performed, for example, by contacting the regulatory element with a plant cell extract, and identifying polypeptides that specifically bind to the regulatory element. Confirmation that the specifically binding polypeptide is a transcription factor can be demonstrated using, for example, the stress-regulated regulatory element operably linked to a reporter gene, and detecting expression of the reporter gene. Control constructs comprising a regulatory element, other than a plant stress-regulated regulatory element, operatively linked to a reporter molecule can be used to confirm that the transcription factor is specific for the plant stress-regulated regulatory element. A polynucleotide encoding such a transcription factor also can be obtained.

The present invention also relates to a method of using a polynucleotide portion of a plant stress-regulated gene to confer a selective advantage on a plant cell. In one embodiment, such a method is performed by introducing a plant stress-regulated regulatory element into a plant cell such as those described herein, wherein, upon exposure of the plant cell to a stress condition to which the regulatory element is responsive, a nucleotide sequence operatively linked to the regulatory element is expressed, thereby conferring a selective advantage to plant cell. The operatively linked nucleotide sequence can be, for example, a transcription factor, the expression of which induces the further expression of polynucleotides involved in a stress response, thereby enhancing the response of a plant to the stress condition. In another embodiment, a coding sequence of a plant stress-regulated gene as disclosed herein is introduced into the cell, thereby providing the plant with a selective advantage in response to a stress condition. In still another embodiment, the method results in the knock-out of a plant stress-regulated gene as disclosed herein in a first population of plants, thereby providing a selective advantage to a stress condition in a second population of plants.

The invention further relates to a method of identifying an agent that modulates the activity of a stress-regulated regulatory element of a plant. In a particular embodiment, is provided a method for identifying an agent that alters the activity of an abiotic stress responsive regulatory element comprising contacting the agent or a composition containing an agent to be tested with at least one abiotic stress responsive regulatory element, preferably selected from the group consisting of SEQ ID NOS:2704–5379 (see Table 2), and determining the effect of the agent on the ability of the regulatory sequence to regulate transcription. In further embodiments, the regulatory elements are associated with particular stresses or combination of stresses such as cold stress (SEQ ID NOS:2704–3955), osmotic stress (SEQ ID NOS:5108–5263), saline stress (SEQ ID NOS:4910–5107), a combination of cold and osmotic stress (SEQ ID NOS:4389–4654), a combination of cold and saline stress (SEQ ID NOS:4655–4909), a combination of osmotic and saline stress (SEQ ID NOS:5264–5379), or a combination of cold, osmotic and saline stress (SEQ ID NOS:3956–4388). In one embodiment, the regulatory element can be operatively linked to a heterologous polynucleotide encoding a reporter molecule, and an agent that modulates the activity of the stress-regulated regulatory element can be identified by detecting a change in expression of the reporter molecule due to contacting the regulatory element with the agent. Such a method can be performed in vitro in a plant cell-free system, or in a plant cell in culture or in a plant in situ. In another embodiment, the agent is contacted with a transgenic plant containing an introduced plant stress-regulated regulatory element, and an agent that modulates the activity of the regulatory element is identified by detecting a phenotypic change in the transgenic plant. The methods of the invention can be performed in the presence or absence of the stress condition to which the particularly regulatory element is responsive.

Another aspect provides a method for identifying an agent that alters abiotic stress responsive polynucleotide expression in a plant or plant cell comprising contacting a plant or plant cell with a test agent; subjecting the plant cell or plant cell to an abiotic stress or combination of stresses before, during or after contact with the agent to be tested; obtaining an expression profile of the plant or plant cell and comparing the expression profile of the plant or plant cell to an expression profile from a plant or plant cell not exposed to the abiotic stress or combination of stresses. In one embodiment, the expression profile comprises expression data for at least one nucleotide sequence comprising any of SEQ ID NOS:1–5379 (see Tables 1 and 2). In additional embodiments, the expression profile comprises expression data for at least one, and preferably two or more sequences associated with a particular abiotic stress or combination of stresses such as cold stress (SEQ ID NOS:1–1261 and 2704–3955), osmotic stress (SEQ ID NOS:2428–2585 and 5108–5263), saline stress (SEQ ID NOS:2227–2427 and 4910–5107), a combination of cold and osmotic stress (SEQ ID NOS:1699–1969 and 4389–4654), a combination of cold and saline stress (SEQ ID NOS:1970–2226 and 4655–4909), a combination of osmotic and saline stress (SEQ ID NOS:2586–2703 and 5264–5379), or a combination of cold, osmotic and saline stress (SEQ ID NOS:1262–1698 and 3956–4388).

Still another aspect provides nucleotide probes useful for detecting an abiotic stress response in plants, the probes comprising a nucleotide sequence of at least 15, 25, 50 or 100 nucleotides that hybridizes under stringent, preferably highly stringent, conditions to at least one sequence comprising any of SEQ ID NOS:1–2703. Also provided are nucleotide probes comprising at least 15, 25, 50 or 100 nucleotides in length that hybridize under stringent, preferably highly stringent conditions, to at least one gene associated with a particular stress or combination of stresses, for example cold stress, (SEQ ID NOS:1–1261), osmotic stress (SEQ ID NOS:2428–2585), saline stress (SEQ ID NOS:2227–2427), a combination of cold and osmotic stress (SEQ ID NOS:1699–1969), a combination of cold and saline stress (SEQ ID NOS: 1970–2226), a combination of osmotic and saline stress (SEQ ID NOS:2586–2703), or a combination of cold, osmotic, and saline stress (SEQ ID NOS:1262–1698).

An additional aspect provides a method for marker-assisted breeding to select plants having an altered resistance to abiotic stress comprising obtaining nucleic acid molecules from the plants to be selected; contacting the nucleic acid molecules with one or more probes that selectively hybridize under stringent, preferably highly stringent, conditions to a nucleic acid sequence selected from the group consisting of SEQ ID NOS:1–2703; detecting the hybridization of the one or more probes to the nucleic acid sequences wherein the presence of the hybridization indicates the presence of a gene associated with altered resistance to abiotic stress; and selecting plants on the basis of the presence or absence of such hybridization. Marker-assisted selection can also be accomplished using one or more probes which selectively hybridize under stringent, preferably highly stringent conditions, to a nucleotide sequence comprising a polynucleotide expressed in response associated with a particular stress, for example, a nucleotide sequence comprising any of SEQ ID NOS:1–1261 (cold stress), SEQ ID NOS:2428–2585 (osmotic stress), SEQ ID NOS:2227–2427 (saline stress), SEQ ID NOS:1699–1969 (cold and osmotic stress), SEQ ID NOS:1970–2226 (cold and saline stress), SEQ ID NOS:2586–2703 (osmotic and saline stress), or SEQ ID NOS:1262–1698 (cold, osmotic and saline stress). In each case marker-assisted selection can be accomplished using a probe or probes to a single sequence or multiple sequences. If multiple sequences are used they can be used simultaneously or sequentially.

A further aspect provides a method for monitoring a population of plants comprising providing at least one sentinel plant containing a recombinant polynucleotide comprising a stress responsive regulatory sequence selected from the group consisting of SEQ ID NOS:2704–5379 which is operatively linked to a nucleotide sequence encoding a detectable marker, for example a fluorescent protein. Additional aspects provide the use of various regulatory sequences including those associated with cold stress (SEQ ID NOS:2704–3955), osmotic stress (SEQ ID NOS:5108–5263), saline stress (SEQ ID NOS:4910–5107), cold and osmotic stress (SEQ ID NOS:4389–4654), cold and saline stress (SEQ ID NOS:4655–4909), osmotic and saline stress (SEQ ID NOS:5264–5379), and cold, osmotic and saline stress (SEQ ID NOS:3956–4388), or fragments thereof wherein such fragments can alter transcription of an operatively linked nucleotide sequence in response to an abiotic stress.

A further aspect provides a computer readable medium having stored thereon computer executable instructions for performing a method comprising receiving data on gene expression in a test plant of at least one nucleic acid molecule having at least 70%, preferably at least 80%, more preferably at least 90%, and most preferably at least 95% nucleotide sequence identity to one or more polynucleotide sequences as set forth in any of SEQ ID NOS:1–2703; and comparing expression data from the test plant to expression data for the same polynucleotide sequence or sequences in a plant that has been exposed to at least one abiotic stress.

Yet a further aspect provides a computer readable medium having stored thereon a data structure comprising, sequence data for at least one, and preferably a plurality of nucleic acid molecules having at least 70%, preferably at least 80%, more preferably at least 90%, and most preferably at least 95% nucleotide sequence identity to a polynucleotide comprising any of SEQ ID NOS:1–2703, or the complement thereof; and a module receiving the nucleic acid molecule sequence data which compares the nucleic acid molecule sequence data to at least one other nucleic acid sequence.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to clusters of genes that are induced in response to one or a combination of abiotic stress conditions. Abiotic stress conditions, such as a shortage or excess of solar energy, water and nutrients, and salinity, high and low temperature, or pollution (e.g., heavy metals), can have a major impact on plant growth and can significantly reduce the yield, for example, of cultivars. Under conditions of abiotic stress, the growth of plant cells is inhibited by arresting the cell cycle in late G1, before DNA synthesis, or at the G2/M boundary (see Dudits, Plant Cell Division, Portland Press Research, Monograph; Francis, Dudits, and Inze, eds., 1997; chap. 2, page 21; Bergounioux, Protoplasma 142:127–136, 1988). The identification of stress-regulated gene clusters, using microarray technology, provides a means to identify plant stress-regulated genes.

As used herein, the term “cluster,” when used in reference to stress-regulated genes, refers to nucleotide sequences of genes that have been selected by drawing Venn diagrams, and selecting those genes that are regulated only by a selected stress condition. In general, a cluster of stress-regulated genes includes at least 5, 10, 15, or 20 genes, including polynucleotide portions thereof, each of which is responsive to the same selected stress condition or conditions. The selected stress condition can be a single stress condition, for example, cold, osmotic stress or salinity stress (see Tables 3–14), or can be a selected combination of stress conditions, for example, cold, osmotic stress and salinity stress (see Tables 15–26). In addition, a cluster can be selected based on specifying that all of the genes are coordinately regulated, for example, they all start at a low level and are induced to a higher level. However, a cluster of saline stress-regulated genes, for example, that was selected for coordinate regulation from low to high, also can be decreased in response to cold or mannitol. By varying the parameters used for selecting a cluster of gene nucleotide sequences, those genes that are expressed in a specific manner following a stress can be identified.

As used herein in reference to a polynucleotide or polynucleotide portion of a gene or nucleic acid molecule, the term “isolated” means a polynucleotide, polynucleotide portion of a gene, or nucleic acid molecule that is free of one or both of the nucleotide sequences that normally flank the polynucleotide in a genome of a naturally-occurring organism from which the polynucleotide is derived. The term includes, for example, a polynucleotide or fragment thereof that is incorporated into a vector or expression cassette; into an autonomously replicating plasmid or virus; into the genomic DNA of a prokaryote or eukaryote; or that exists as a separate molecule independent of other polynucleotides. It also includes a recombinant polynucleotide that is part of a hybrid polynucleotide, for example, one encoding a polypeptide sequence.

The terms “polynucleotide,” “oligonucleotide,” and “nucleic acid sequence” are used interchangeably herein to refer to a polymeric (2 or more monomers) form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. Although nucleotides are usually joined by phosphodiester linkages, the term also includes polymers containing neutral amide backbone linkages composed of aminoethyl glycine units. The terms are used only to refer to the primary structure of the molecule. Thus, the term includes double stranded and single stranded DNA molecules, including a sense strand or an antisense strand, and RNA molecules as well as genomic DNA, cDNA, mRNA and the like. It will be recognized that such polynucleotides can be modified, for example, by including a label such as a radioactive, fluorescent or other tag, by methylation, by the inclusion of a cap structure, by containing a substitution of one or more of the naturally occurring nucleotides with a nucleotide analog, by containing an internucleotide modification such as having uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoramidates, carbamates, or the like), by containing a pendant moiety such as a protein (e.g., a nuclease, toxin, antibody, signal peptide, poly-L-lysine, or the like), by containing an intercalator such as acridine or psoralen, by containing a chelator, which can be a metal such as boron, an oxidative metal, or a radioactive metal, by containing an alkylator, or by having a modified linkage (e.g., an alpha anomeric nucleic acid).

The term “recombinant nucleic acid molecule” refers to a polynucleotide produced by human intervention. A recombinant nucleic acid molecule can contain two or more nucleotide sequences that are linked in a manner such that the product is not found in a cell in nature. In particular, the two or more nucleotide sequences can be operatively linked and, for example, can encode a fusion polypeptide, or can comprise a nucleotide sequence and a regulatory element. A recombinant nucleic acid molecule also can be based on, but different, from a naturally occurring polynucleotide, for example, a polynucleotide having one or more nucleotide changes such that a first codon, which normally is found in the polynucleotide, is replaced with a degenerate codon that encodes the same or a conservative amino acid, or such that a sequence of interest is introduced into the polynucleotide, for example, a restriction endonuclease recognition site or a splice site, a promoter, a DNA replication initiation site, or the like.

As used herein, the term “abiotic stress” or “abiotic stress condition” refers to the exposure of a plant, plant cell, or the like, to a non-living (“abiotic”) physical or chemical agent or condition that has an adverse effect on metabolism, growth, development, propagation and/or survival of the plant (collectively “growth”). An abiotic stress can be imposed on a plant due, for example, to an environmental factor such as water (e.g., flooding, drought, dehydration), anaerobic conditions (e.g., a low level of oxygen), abnormal osmotic conditions, salinity or temperature (e.g., hot/heat, cold, freezing, frost), a deficiency of nutrients or exposure to pollutants, or by a hormone, second messenger or other molecule. Anaerobic stress, for example, is due to a reduction in oxygen levels (hypoxia or anoxia) sufficient to produce a stress response. A flooding stress can be due to prolonged or transient immersion of a plant, plant part, tissue or isolated cell in a liquid medium such as occurs during monsoon, wet season, flash flooding or excessive irrigation of plants, or the like. A cold stress or heat stress can occur due to a decrease or increase, respectively, in the temperature from the optimum range of growth temperatures for a particular plant species. Such optimum growth temperature ranges are readily determined or known to those skilled in the art. Dehydration stress can be induced by the loss of water, reduced turgor, or reduced water content of a cell, tissue, organ or whole plant. Drought stress can be induced by or associated with the deprivation of water or reduced supply of water to a cell, tissue, organ or organism. Saline stress (salt stress) can be associated with or induced by a perturbation in the osmotic potential of the intracellular or extracellular environment of a cell. Osmotic stress also can be associated with or induced by a change, for example, in the concentration of molecules in the intracellular or extracellular environment of a plant cell, particularly where the molecules cannot be partitioned across the plant cell membrane.

As disclosed herein, clusters of plant stress-regulated genes (Example 1; see, also, Tables 1–31) and homologs and orthologs thereof (Table 32) have been identified. Remarkably, several of the stress-regulated genes previously were known to encode polypeptides having defined cellular functions, including roles as transcription factors, enzymes such as kinases, and structural proteins such as channel proteins (see Tables 29–31). The identification of Arabidopsis stress-regulated genes provides a means to identify homologous and orthologous genes and gene sequences in other plant species using well known procedures and algorithms based on identity (or homology) to the disclosed sequences. Thus, the invention provides polynucleotide sequences comprising plant stress-regulated genes that are homologs or orthologs, variants, or otherwise substantially similar to the polynucleotides disclosed herein, and having an E value≦1×10⁻⁸, which can be identified, for example, by a BLASTN search using the Arabidopsis polynucleotides of Tables 1 and 2 (SEQ ID NOS:1–5379) as query sequences (see Table 32, on CD).

A polynucleotide sequence of a stress-regulated gene as disclosed herein can be particularly useful for performing the methods of the invention on a variety of plants, including but not limited to, corn (Zea mays), Brassica sp. (e.g., B. napus, B. rapa, B. juncea), particularly those Brassica species useful as sources of seed oil, alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), millet (e.g., pearl millet (Pennisetum glaucum), proso millet (Panicum miliaceum), foxtail millet (Setaria italica), finger millet (Eleusine coracana)), sunflower (Helianthus annuus), safflower (Carthamus tinctorius), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium barbadense, Gossypium hirsutum), sweet potato (Ipomoea batatus), cassava (Manihot esculenta), coffee (Cofea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea ultilane), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), sugarcane (Saccharum spp.), oats, duckweed (Lemna), barley, tomatoes (Lycopersicon esculentum), lettuce (e.g., Lactuca sativa), green beans (Phaseolus vulgaris), lima beans (Phaseolus limensis), peas (Lathyrus spp.), and members of the genus Cucumis such as cucumber (C. sativus), cantaloupe (C. cantalupensis), and musk melon (C. melo). Ornamentals such as azalea (Rhododendron spp.), hydrangea (Macrophylla hydrangea), hibiscus (Hibiscus rosasanensis), roses (Rosa spp.), tulips (Tulipa spp.), daffodils (Narcissus spp.), petunias (Petunia hybrida), carnation (Dianthus caryophyllus), poinsettia (Euphorbia pulcherrima), and chrysanthemum are also included. Additional ornamentals within the scope of the invention include impatiens, Begonia, Pelargonium, Viola, Cyclamen, Verbena, Vinca, Tagetes, Primula, Saint Paulia, Agertum, Amaranthus, Antihirrhinum, Aquilegia, Cineraria, Clover, Cosmo, Cowpea, Dahlia, Datura, Delphinium, Gerbera, Gladiolus, Gloxinia, Hippeastrum, Mesembryanthemum, Salpiglossos, and Zinnia. Conifers that may be employed in practicing the present invention include, for example, pines such as loblolly pine (Pinus taeda), slash pine (Pinus elliotii), ponderosa pine (Pinus ponderosa), lodgepole pine (Pinus contorta), and Monterey pine (Pinus radiata), Douglas-fir (Pseudotsuga menziesii); Western hemlock (Tsuga ultilane); Sitka spruce (Picea glauca); redwood (Sequoia sempervirens); true firs such as silver fir (Abies amabilis) and balsam fir (Abies balsamea); and cedars such as Western red cedar (Thuja plicata) and Alaska yellow-cedar (Chamaecyparis nootkatensis).

Leguminous plants which may be used in the practice of the present invention include beans and peas. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mung bean, lima bean, fava bean, lentils, chickpea, etc. Legumes include, but are not limited to, Arachis, e.g., peanuts, Vicia, e.g., crown vetch, hairy vetch, adzuki bean, mung bean, and chickpea, Lupinus, e.g., lupine, trifolium, Phaseolus, e.g., common bean and lima bean, Pisum, e.g., field bean, Melilotus, e.g., clover, Medicago, e.g., alfalfa, Lotus, e.g., trefoil, lens, e.g., lentil, and false indigo. Preferred forage and turf grass for use in the methods of the invention include alfalfa, orchard grass, tall fescue, perennial ryegrass, creeping bent grass, and redtop.

Other plants within the scope of the invention include Acacia, aneth, artichoke, arugula, blackberry, canola, cilantro, clementines, escarole, eucalyptus, fennel, grapefruit, honey dew, jicama, kiwifruit, lemon, lime, mushroom, nut, okra, orange, parsley, persimmon, plantain, pomegranate, poplar, radiata pine, radicchio, Southern pine, sweetgum, tangerine, triticale, vine, yams, apple, pear, quince, cherry, apricot, melon, hemp, buckwheat, grape, raspberry, chenopodium, blueberry, nectarine, peach, plum, strawberry, watermelon, eggplant, pepper, cauliflower, Brassica, e.g., broccoli, cabbage, ultilan sprouts, onion, carrot, leek, beet, broad bean, celery, radish, pumpkin, endive, gourd, garlic, snapbean, spinach, squash, turnip, ultilane, chicory, groundnut and zucchini.

As used herein, the term “substantially similar”, when used herein with respect to a nucleotide sequence, means a nucleotide sequence corresponding to a reference nucleotide sequence, wherein the corresponding sequence encodes a polypeptide or comprises a regulatory element having substantially the same structure and function as the polypeptide encoded by the reference nucleotide sequence, for example, where only changes in amino acids not affecting the polypeptide function occur. For purposes of the present invention, a reference (or query) sequence is a polynucleotide sequence as set forth in any of SEQ ID NOS:1–2703 or a polypeptide encoded thereby. Desirably, a substantially similar nucleotide sequence encodes the polypeptide encoded by the reference nucleotide sequence. The percentage of identity between the substantially similar nucleotide sequence and the reference nucleotide sequence desirably is at least 60%, more desirably at least 75%, preferably at least 90%, more preferably at least 95%, still more preferably at least 99% and including 100%. A nucleotide sequence is “substantially similar” to reference nucleotide sequence hybridizes to the reference nucleotide sequence in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO₄, 1 mM EDTA at 50° C. with washing in 2×SSC, 0.1% SDS at 50° C., more desirably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO₄, 1 mM EDTA at 50° C. with washing in 1×SSC, 0.1% SDS at 50° C. (stringent conditions), more desirably still in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO₄, 1 mM EDTA at 50° C. with washing in 0.5×SSC, 0.1% SDS at 50° C. (high stringency), preferably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO₄, 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 50° C. (very high stringency), more preferably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO₄, 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 65° C. (extremely high stringency).

In addition, the term “substantially similar,” when used in reference to a polypeptide sequence, means that an amino acid sequence relative to a reference (query) sequence shares at least about 65% amino acid sequence identity, particularly at least about 75% amino acid sequence identity, and preferably at least about 85%, more preferably at least about 90%, and most preferably at least about 95% or greater amino acid sequence identity. Generally, sequences having an E≦10⁻⁸are considered to be substantially similar to a query sequence. Such sequence identity can take into account conservative amino acid changes that do not substantially affect the function of a polypeptide. As such, homologs or orthologs of the Arabidopsis stress-regulated nucleotide sequences disclosed herein, variants thereof, and polypeptides substantially similar to the polynucleotide sequence of Arabidopsis stress-regulated genes set forth in SEQ ID NOS:1–5379 are encompassed within the present invention and, therefore, useful for practicing the methods of the invention (see, for example, Table 32, which is on the CD-R filed herewith, and incorporated herein by reference).

Homology or identity is often measured using sequence analysis software such as the Sequence Analysis Software Package of the Genetics Computer Group (University of Wisconsin Biotechnology Center, 1710 University Avenue, Madison, Wis. 53705). Such software matches similar sequences by assigning degrees of homology to various deletions, substitutions and other modifications. The terms “homology” and “identity,” when used herein in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or of nucleotides that are the same when compared and aligned for maximum correspondence over a comparison window or designated region as measured using any number of sequence comparison algorithms or by manual alignment and visual inspection.

For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters.

The term “comparison window” is used broadly herein to include reference to a segment of any one of the number of contiguous positions, for example, about 20 to 600 positions, for example, amino acid or nucleotide position, usually about 50 to about 200 positions, more usually about 100 to about 150 positions, in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequence for comparison are well-known in the art. Optimal alignment of sequences for comparison can be conducted, for example, by the local homology algorithm of Smith and Waterman (Adv. Appl. Math. 2:482, 1981), by the homology alignment algorithm of Needleman and Wunsch (J. Mol. Biol. 48:443, 1970), by the search for similarity method of Person and Lipman (Proc. Natl. Acad. Sci., USA 85:2444, 1988), each of which is incorporated herein by reference; by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.); or by manual alignment and visual inspection. Other algorithms for determining homology or identity include, for example, in addition to a BLAST program (Basic Local Alignment Search Tool at the National Center for Biological Information), ALIGN, AMAS (Analysis of Multiply Aligned Sequences), AMPS (Protein Multiple Sequence Alignment), ASSET (Aligned Segment Statistical Evaluation Tool), BANDS, BESTSCOR, BIOSCAN (Biological Sequence Comparative Analysis Node), BLIMPS (BLocks IMProved Searcher), FASTA, Intervals & Points, BMB, CLUSTAL V, CLUSTAL W, CONSENSUS, LCONSENSUS, WCONSENSUS, Smith-Waterman algorithm, DARWIN, Las Vegas algorithm, FNAT (Forced Nucleotide Alignment Tool), Framealign, Framesearch, DYNAMIC, FILTER, FSAP (Fristensky Sequence Analysis Package), GAP (Global Alignment Program), GENAL, GIBBS, GenQuest, ISSC (Sensitive Sequence Comparison), LALIGN (Local Sequence Alignment), LCP (Local Content Program), MACAW (Multiple Alignment Construction & Analysis Workbench), MAP (Multiple Alignment Program), MBLKP, MBLKN, PIMA (Pattern-Induced Multi-sequence Alignment), SAGA (Sequence Alignment by Genetic Algorithm) and WHAT-IF. Such alignment programs can also be used to screen genome databases to identify polynucleotide sequences having substantially identical sequences.

A number of genome databases are available for comparison. Several databases containing genomic information annotated with some functional information are maintained by different organizations, and are accessible via the internet, for example, at world wide web addresses (url's) “wwwtigr.org/tdb”; “genetics.wisc.edu”; “genome-www.stanford.edu/˜ball”; “hiv-web.lanl.gov”; “ncbi.nlm.nih.gov”; “ebi.ac.uk”; “Pasteur.fr/other/biology”; and “genome.wi.mit.edu”.

In particular, the BLAST and BLAST 2.0 algorithms using default parameters are particularly useful for identifying polynucleotide and polypeptides encompassed within the present invention (Altschul et al. (Nucleic Acids Res. 25:3389–3402, 1977; J. Mol. Biol. 215:403–410, 1990, each of which is incorporated herein by reference). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra, 1977, 1990). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, M=5, N=4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength of 3, and expectations (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff and Henikoff, Proc. Natl. Acad. Sci., USA 89:10915, 1989) alignments (B) of 50, expectation (E) of 10, M=5, N=4, and a comparison of both strands.

The BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, for example, Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873, 1993, which is incorporated herein by reference). One measure of similarity provided by BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a references sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01, and most preferably less than about 0.001. Significantly, upon identifying polynucleotides that are substantially similar to those of SEQ ID NOS:1–5379, the identified polynucleotides can be used as query sequences in a BLAST search to identify polynucleotides and polypeptides substantially similar thereto.

It should be noted that the nucleotide sequences set forth as SEQ ID NOS:1–2703 comprise coding sequences, whereas the nucleotide sequences set forth as SEQ ID NOS:2704–5379 comprise regulatory sequences. In addition, the coding sequences and regulatory sequences are related in that, for example, SEQ ID NO:1 is the coding sequence of a plant cold regulated gene having a 5′ upstream (regulatory) sequence set forth as SEQ ID NO:2704 (see Table 2). Similarly, SEQ ID NO:2705 comprises a regulatory region of SEQ ID NO:2, SEQ ID NO:2706 comprises a regulatory region of SEQ ID NO:3, and so forth as shown in Table 2. As such, reference herein, for example, to a “polynucleotide comprising SEQ ID NO:1” can, unless indicated otherwise, include at least SEQ ID NO:2704. In some cases, the entire coding region of a plant stress regulated gene or the 5′ upstream sequence has not yet been determined (see, for example, SEQ ID NO:43 in Table 3, where “none” indicates that 5′ upstream regulatory sequences have not yet been determined). However, the determination of a complete coding sequence where only a portion is known or of regulatory sequences where a portion of the coding sequence is known can be made using methods as disclosed herein or otherwise known in the art.

In one embodiment, protein and nucleic acid sequence homologies are evaluated using the Basic Local Alignment Search Tool (“BLAST”). In particular, five specific BLAST programs are used to perform the following task:

- (1) BLASTP and BLAST3 compare an amino acid query sequence against a protein sequence database;
- (2) BLASTN compares a nucleotide query sequence against a nucleotide sequence database;
- (3) BLASTX compares the six-frame conceptual translation products of a query nucleotide sequence (both strands) against a protein sequence database;
- (4) TBLASTN compares a query protein sequence against a nucleotide sequence database translated in all six reading frames (both strands); and
- (5) TBLASTX compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database.

The BLAST programs identify homologous sequences by identifying similar segments, which are referred to herein as “high-scoring segment pairs,” between a query amino or nucleic acid sequence and a test sequence which is preferably obtained from a protein or nucleic acid sequence database. High-scoring segment pairs are preferably identified (i.e., aligned) by means of a scoring matrix, many of which are known in the art. Preferably, the scoring matrix used is the BLOSUM62 matrix (Gonnet et al., Science 256:1443–1445, 1992; Henikoff and Henikoff, Proteins 17:49–61, 1993, each of which is incorporated herein by reference). Less preferably, the PAM or PAM250 matrices may also be used (Schwartz and Dayhoff, eds., “Matrices for Detecting Distance Relationships: Atlas of Protein Sequence and Structure” (Washington, National Biomedical Research Foundation 1978)). BLAST programs are accessible through the U.S. National Library of Medicine, for example, on the world wide web at address (url) “ncbi.nlm.nih.gov”.

The parameters used with the above algorithms may be adapted depending on the sequence length and degree of homology studied. In some embodiments, the parameters may be the default parameters used by the algorithms in the absence of instructions from the user.

The term “substantially similar” also is used in reference to a comparison of expression profiles of nucleotide sequences, wherein a determination that an expression profile characteristic of a stress response is substantially similar to the profile of nucleic acid molecules expressed in a plant cell being examined (“test plant”) is indicative of exposure of the test plant cell to one or a combination of abiotic stress conditions. When used in reference to such a comparison of expression profiles, the term “substantially similar” means that the individual nucleotide sequences in the test plant cell profile are altered in the same manner as the corresponding nucleotide sequences in the expression profile characteristic of the stress response.

By way of example, where exposure to saline results in an increased expression of nucleotide sequences A, B and C, and a decreased expression of nucleotide sequences D and E, as indicated by the expression profile characteristic of a saline stress response, a determination that corresponding nucleotide sequences A, B and C in the test plant cell are increased and that nucleotides sequences D and E are decreased is indicative of exposure of the test plant cell to a saline stress condition. It should be recognized that, where, for example, only nucleotide sequences A, B, D and E are examined in the test plant cell, an increase in A and B and a decrease in D and E expression of the test plant cells is considered to be substantially similar to the expression profile characteristic of a saline stress condition and, therefore, is indicative of exposure of the plant cell to a saline stress condition. Similarly, where the levels of expression of the nucleotide sequences examined in a test plant are altered in the same manner, i.e., are increased or are decreased, as that observed in an expression profile characteristic of a particular stress response, the absolute levels of expression may vary, for example, two-fold, five-fold, ten-fold, or the like. Nevertheless, the expression profile of the test plant cell is considered to be substantially similar to the expression profile characteristic of the particular stress response and, therefore, indicative of exposure of the plant cell to the stress condition.

As disclosed herein, clusters of stress-regulated genes (and their products), some of which also have been described as having cellular functions such as enzymatic activity or roles as transcription factors, are involved in the response of plant cells to various abiotic stresses (see Tables 29–31; see, also, Tables 1 and 32). As such, the polynucleotide sequences comprising the genes in a cluster likely share common stress-regulated regulatory elements, including, for example, cold-regulated regulatory elements (SEQ ID NOS:2704–3955), salinity-regulated regulatory elements (SEQ ID NOS:4910–5107, and osmotic pressure-regulated regulatory elements (SEQ ID NO:5108–5263), as well as regulatory elements that are responsive to a combination of stress conditions, but not to any of the individual stress conditions, alone (SEQ ID NOS:3956–4909 and 5263–5379). The identification of such clusters of genes thus provides a means to identify the stress-regulated regulatory elements that control the level of expression of these genes.

As used herein, the term “plant stress-regulated gene” means a polynucleotide sequence of a plant, the transcription of which is altered in response to exposure to a stress condition, and the regulatory elements linked to such a polynucleotide sequence and involved in the stress response, which can be induction or repression. In general, plant stress gene regulatory elements are contained within a sequence including approximately two kilobases upstream (5′) of the transcription or translation start site and two kilobases downstream (3′) of the transcription or translation termination site. In the absence of an abiotic stress condition, the stress-regulated gene can normally be unexpressed in the cells, can be expressed at a basal level, which is induced to a higher level in response to the stress condition, or can be expressed at a level that is reduced (decreased) in response to the stress condition. The coding region of a plant stress-regulated gene encodes a stress-regulated polypeptide, and also can be the basis for expression of a functional RNA molecule such as an antisense molecule or ribozyme. A stress-regulated polypeptide can have an adaptive effect on a plant, thereby allowing the plant to better tolerate stress conditions; or can have a maladaptive effect, thereby decreasing the ability of the plant to tolerate the stress conditions.

The present invention provides an isolated plant stress-regulated regulatory element, which regulates expression of an operatively linked nucleotide sequence in a plant in response a stress condition. As disclosed herein, a plant stress-regulated regulatory element can be isolated from a polynucleotide sequence of a plant stress-regulated gene comprising a nucleotide sequence as set forth in SEQ ID NOS:1–2703, for example any of SEQ ID NOS:2704–5379 (see Table 2). It is recognized that certain of the polynucleotides set forth as SEQ ID NOS:1–5379 previously have been described as being involved in a stress-regulated response in plants, including SEQ ID NOS:156, 229, 233, 558, 573, 606, 625, 635, 787, 813, 1263, 1386, 1391, 1405, 1445, 1484, 1589, 1609, 1634, 1726, 1866, 1918, and 1928 and, therefore, are not encompassed, in whole or in part, within the compositions of the invention, and are encompassed within only certain particular methods of the invention, for example, methods of making a transgenic plant that is resistant to two or more stress conditions, since, even where such a gene was known to be expressed in response to a single stress condition such as cold or saline (e.g., SEQ ID NO:1263), it was not known prior to the present disclosure that any of these genes was responsive to a combination of stress conditions (for example, a combination of cold and osmotic stress for SEQ ID NOS:1726, 1866, 1918, and 1928; or a combination of cold, osmotic and saline stress for SEQ ID NOS:1263, 1386, 1391, 1405, 1445, 1484, 1589, 1609, and 1634).

Methods for identifying and isolating the stress-regulated regulatory element from the disclosed polynucleotides, or genomic DNA clones corresponding thereto, are well known in the art. For example, methods of making deletion constructs or linker-scanner constructs can be used to identify nucleotide sequences that are responsive to a stress condition. Generally, such constructs include a reporter gene operatively linked to the sequence to be examined for regulatory activity. By performing such assays, a plant stress-regulated regulatory element can be defined within a sequence of about 500 nucleotides or fewer, generally at least about 200 nucleotides or fewer, particularly about 50 to 100 nucleotides, and more particularly at least about 20 nucleotides or fewer. Preferably the minimal (core) sequence required for regulating a stress response of a plant is identified.

The nucleotide sequences of the genes of a cluster also can be examined using a homology search engine such as described herein to identify sequences of conserved identity, particularly in the nucleotide sequence upstream of the transcription start site. Since all of the genes in a cluster as disclosed are induced in response to a particular stress condition or a particular combination of stress conditions, some or all of the nucleotide sequences can share conserved stress-regulated regulatory elements. By performing such a homology search, putative stress-regulated regulatory elements can be identified. The ability of such identified sequences to function as a plant stress-regulated regulatory element can be confirmed, for example, by operatively linking the sequence to a reporter gene and assaying the construct for responsiveness to a stress condition.

As used herein, the term “regulatory element” means a nucleotide sequence that, when operatively linked to a coding region of a gene, effects transcription of the coding region such that a ribonucleic acid (RNA) molecule is transcribed from the coding region. A regulatory element generally can increase or decrease the amount of transcription of a nucleotide sequence, for example, a coding sequence, operatively linked to the element with respect to the level at which the nucleotide sequence would be transcribed absent the regulatory element. Regulatory elements are well known in the art and include promoters, enhancers, silencers, inactivated silencer intron sequences, 3′-untranslated or 5′-untranslated sequences of transcribed sequence, for example, a poly-A signal sequence, or other protein or RNA stabilizing elements, or other gene expression control elements known to regulate gene expression or the amount of expression of a gene product. A regulatory element can be isolated from a naturally occurring genomic DNA sequence or can be synthetic, for example, a synthetic promoter.

Regulatory elements can be constitutively expressed regulatory element, which maintain gene expression at a relative level of activity (basal level), or can be regulated regulatory elements. Constitutively expressed regulatory elements can be expressed in any cell type, or can be tissue specific, which are expressed only in particular cell types, phase specific, which are expressed only during particular developmental or growth stages of a plant cell, or the like. A regulatory element such as a tissue specific or phase specific regulatory element or an inducible regulatory element useful in constructing a recombinant polynucleotide or in a practicing a method of the invention can be a regulatory element that generally, in nature, is found in a plant genome. However, the regulatory element also can be from an organism other than a plant, including, for example, from a plant virus, an animal virus, or a cell from an animal or other multicellular organism.

A regulatory element useful for practicing method of the present is a promoter element. Useful promoters include, but are not limited to, constitutive, inducible, temporally regulated, developmentally regulated, spatially-regulated, chemically regulated, stress-responsive, tissue-specific, viral and synthetic promoters. Promoter sequences are known to be strong or weak. A strong promoter provides for a high level of gene expression, whereas a weak promoter provides for a very low level of gene expression. An inducible promoter is a promoter that provides for the turning on and off of gene expression in response to an exogenously added agent, or to an environmental or developmental stimulus. A bacterial promoter such as the P_tacpromoter can be induced to varying levels of gene expression depending on the level of isothiopropylgalactoside added to the transformed bacterial cells. An isolated promoter sequence that is a strong promoter for heterologous nucleic acid is advantageous because it provides for a sufficient level of gene expression to allow for easy detection and selection of transformed cells and provides for a high level of gene expression when desired.

Within a plant promoter region there are several domains that are necessary for full function of the promoter. The first of these domains lies immediately upstream of the structural gene and forms the “core promoter region” containing consensus sequences, normally 70 base pairs immediately upstream of the gene. The core promoter region contains the characteristic CAAT and TATA boxes plus surrounding sequences, and represents a transcription initiation sequence that defines the transcription start point for the structural gene.

The presence of the core promoter region defines a sequence as being a promoter: if the region is absent, the promoter is non-functional. The core promoter region, however, is insufficient to provide full promoter activity. A series of regulatory sequences upstream of the core constitute the remainder of the promoter. These regulatory sequences determine expression level, the spatial and temporal pattern of expression and, for an important subset of promoters, expression under inductive conditions (regulation by external factors such as light, temperature, chemicals, hormones).

To define a minimal promoter region, a DNA segment representing the promoter region is removed from the 5′ region of the gene of interest and operably linked to the coding sequence of a marker (reporter) gene by recombinant DNA techniques well known to the art. The reporter gene is operably linked downstream of the promoter, so that transcripts initiating at the promoter proceed through the reporter gene. Reporter genes generally encode proteins which are easily measured, including, but not limited to, chloramphenicol acetyl transferase (CAT), beta-glucuronidase (GUS), green fluorescent protein (GFP), β-galactosidase (β-GAL), and luciferase.

The construct containing the reporter gene under the control of the promoter is then introduced into an appropriate cell type by transfection techniques well known to the art. To assay for the reporter protein, cell lysates are prepared and appropriate assays, which are well known in the art, for the reporter protein are performed. For example, if CAT were the reporter gene of choice, the lysates from cells transfected with constructs containing CAT under the control of a promoter under study are mixed with isotopically labeled chloramphenicol and acetyl-coenzyme A (acetyl-CoA). The CAT enzyme transfers the acetyl group from acetyl-CoA to the 2-position or 3-position of chloramphenicol. The reaction is monitored by thin layer chromatography, which separates acetylated chloramphenicol from unreacted material. The reaction products are then visualized by autoradiography.

The level of enzyme activity corresponds to the amount of enzyme that was made, which in turn reveals the level of expression from the promoter of interest. This level of expression can be compared to other promoters to determine the relative strength of the promoter under study. In order to be sure that the level of expression is determined by the promoter, rather than by the stability of the mRNA, the level of the reporter mRNA can be measured directly, for example, by northern blot analysis.

Once activity is detected, mutational and/or deletional analyses may be employed to determine the minimal region and/or sequences required to initiate transcription. Thus, sequences can be deleted at the 5′ end of the promoter region and/or at the 3′ end of the promoter region, and nucleotide substitutions introduced. These constructs are then introduced to cells and their activity determined.

The choice of promoter will vary depending on the temporal and spatial requirements for expression, and also depending on the target species. In some cases, expression in multiple tissues is desirable. While in others, tissue-specific, e.g., leaf-specific, seed-specific, petal-specific, anther-specific, or pith-specific, expression is desirable. Although many promoters from dicotyledons have been shown to be operational in monocotyledons and vice versa, ideally dicotyledonous promoters are selected for expression in dicotyledons, and monocotyledonous promoters for expression in monocotyledons. There is, however, no restriction to the origin or source of a selected promoter. It is sufficient that the promoters are operational in driving the expression of a desired nucleotide sequence in the particular cell.

A range of naturally-occurring promoters are known to be operative in plants and have been used to drive the expression of heterologous (both foreign and endogenous) genes and nucleotide sequences in plants: for example, the constitutive 35S cauliflower mosaic virus (CaMV) promoter, the ripening-enhanced tomato polygalacturonase promoter (Bird et al., 1988), the E8 promoter (Diekman and Fischer, 1988) and the fruit specific 2A1 promoter (Pear et al., 1989). Many other promoters, e.g., U2 and U5 snRNA promoters from maize, the promoter from alcohol dehydrogenase, the Z4 promoter from a gene encoding the Z4 22 kD zein protein, the Z10 promoter from a gene encoding a 10 kD zein protein, a Z27 promoter from a gene encoding a 27 kD zein protein, the A20 promoter from the gene encoding a 19 kD zein protein, inducible promoters, such as the light inducible promoter derived from the pea rbcS gene and the actin promoter from rice, e.g., the actin 2 promoter (WO 00/70067); seed specific promoters, such as the phaseolin promoter from beans, may also be used. The nucleotide sequences of the stress-regulated genes of this invention can also be expressed under the regulation of promoters that are chemically regulated. This enables the nucleic acid sequence or encoded polypeptide to be synthesized only when the crop plants are treated with the inducing chemicals. Chemical induction of gene expression is detailed in EP 0 332 104 and U.S. Pat. No. 5,614,395.

In some instances it may be desirable to link a constitutive promoter to a polynucleotide comprising a stress regulated gene of the invention. Examples of some constitutive promoters include the rice actin 1 (Wang et al., 1992; U.S. Pat. No. 5,641,876), CaMV 35S (Odell et al., 1985), CaMV 19S (Lawton et al., 1987), nos, Adh, sucrose synthase; and the ubiquitin promoters.

In other situations it may be desirable to limit expression of stress-related sequences to specific tissues or stages of development. As used herein, the term “tissue specific or phase specific regulatory element” means a nucleotide sequence that effects transcription in only one or a few cell types, or only during one or a few stages of the life cycle of a plant, for example, only for a period of time during a particular stage of growth, development or differentiation. The terms “tissue specific” and “phase specific” are used together herein in referring to a regulatory element because a single regulatory element can have characteristics of both types of regulatory elements. For example, a regulatory element active only during a particular stage of plant development also can be expressed only in one or a few types of cells in the plant during the particular stage of development. As such, any attempt to classify such regulatory elements as tissue specific or as phase specific can be difficult. Accordingly, unless indicated otherwise, all regulatory elements having the characteristic of a tissue specific regulatory element, or a phase specific regulatory element, or both are considered together for purposes of the present invention.

Examples of tissue specific promoters which have been described include the lectin (Vodkin, 1983; Lindstrom et al., 1990) corn alcohol dehydrogenase 1 (Vogel et al., 1989; Dennis et al., 1984), corn light harvesting complex (Simpson, 1986; Bansal et al., 1992), corn heat shock protein (Odell et al., 1985), pea small subunit RuBP carboxylase (Poulsen et al., 1986), Ti plasmid mannopine synthase and Ti plasmid nopaline synthase (Langridge et al., 1989), petunia chalcone isomerase (vanTunen et al., 1988), bean glycine rich protein 1 (Keller et al., 1989), truncated CaMV 35s (Odell et al., 1985), potato patatin (Wenzler et al., 1989), root cell (Yamamoto et al., 1990), maize zein (Reina et al., 1990; Kriz et al., 1987; Wandelt et al., 1989; Langridge et al., 1983; Reina et al., 1990), globulin-1 (Belanger et al., 1991), α-tubulin, cab (Sullivan et al., 1989), PEPCase (Hudspeth & Grula, 1989), R gene complex-associated promoters (Chandler et al., 1989), histone, and chalcone synthase promoters (Franken et al., 1991). Tissue specific enhancers are described by Fromm et al. (1989).

Several other tissue-specific regulated genes and/or promoters have been reported in plants, including genes encoding seed storage proteins such as napin, cruciferin, beta-conglycinin, and phaseolin, zein or oil body proteins such as oleosin, genes involved in fatty acid biosynthesis, including acyl carrier protein, stearoyl-ACP desaturase, fatty acid desaturases (fad 2-1), and other genes expressed during embryonic development such as Bce4 (see, for example, EP 255378 and Kridl et al., 1991). Particularly useful for seed-specific expression is the pea vicilin promoter (Czako et al., 1992). (See also U.S. Pat. No. 5,625,136, which is incorporated herein by reference.) Other useful promoters for expression in mature leaves are those that are switched on at the onset of senescence, such as the SAG promoter from Arabidopsis (Gan et al., 1995).

A class of fruit-specific promoters expressed at or during antithesis through fruit development, at least until the beginning of ripening, is discussed in U.S. Pat. No. 4,943,674. cDNA clones that are preferentially expressed in cotton fiber have been isolated (John et al., 1992). cDNA clones from tomato displaying differential expression during fruit development have been isolated and characterized (Mansson et al., 1985, Slater et al., 1985). The promoter for polygalacturonase gene is active in fruit ripening. The polygalacturonase gene is described in U.S. Pat. Nos. 4,535,060, 4,769,061, 4,801,590, and 5,107,065, each of which is incorporated herein by reference.

Other examples of tissue-specific promoters include those that direct expression in leaf cells following damage to the leaf (for example, from chewing insects), in tubers (for example, patatin gene promoter), and in fiber cells (an example of a developmentally-regulated fiber cell protein is E6 (John et al., 1992). The E6 gene is most active in fiber, although low levels of transcripts are found in leaf, ovule and flower.

Additional tissue specific or phase specific regulatory elements include, for example, the AGL8/FRUITFULL regulatory element, which is activated upon floral induction (Hempel et al., Development 124:3845–3853, 1997, which is incorporated herein by reference); root specific regulatory elements such as the regulatory elements from the RCP1 gene and the LRP1 gene (Tsugeki and Fedoroff, Proc. Natl. Acad., USA 96:12941–12946, 1999; Smith and Fedoroff, Plant Cell 7:735–745, 1995, each of which is incorporated herein by reference); flower specific regulatory elements such as the regulatory elements from the LEAFY gene and the APETELA1 gene (Blazquez et al., Development 124:3835–3844, 1997, which is incorporated herein by reference; Hempel et al., supra, 1997); seed specific regulatory elements such as the regulatory element from the oleosin gene (Plant et al., Plant Mol. Biol. 25:193–205, 1994, which is incorporated herein by reference), and dehiscence zone specific regulatory element. Additional tissue specific or phase specific regulatory elements include the Zn13 promoter, which is a pollen specific promoter (Hamilton et al., Plant Mol. Biol. 18:211–218, 1992, which is incorporated herein by reference); the UNUSUAL FLORAL ORGANS (UFO) promoter, which is active in apical shoot meristem; the promoter active in shoot meristems (Atanassova et al., Plant J. 2:291, 1992, which is incorporated herein by reference), the cdc2a promoter and cyc07 promoter (see, for example, Ito et al., Plant Mol. Biol. 24:863, 1994; Martinez et al., Proc. Natl. Acad. Sci., USA 89:7360, 1992; Medford et al., Plant Cell 3:359, 1991; Terada et al., Plant J. 3:241, 1993; Wissenbach et al., Plant J. 4:411, 1993, each of which is incorporated herein by reference); the promoter of the APETELA3 gene, which is active in floral meristems (Jack et al., Cell 76:703, 1994, which is incorporated herein by reference; Hempel et al., supra, 1997); a promoter of an agamous-like (AGL) family member, for example, AGL8, which is active in shoot meristem upon the transition to flowering (Hempel et al., supra, 1997); floral abscission zone promoters; L1-specific promoters; and the like.

The tissue-specificity of some “tissue-specific” promoters may not be absolute and may be tested by one skilled in the art using the diphtheria toxin sequence. One can also achieve tissue-specific expression with “leaky” expression by a combination of different tissue-specific promoters (Beals et al., 1997). Other tissue-specific promoters can be isolated by one skilled in the art (see U.S. Pat. No. 5,589,379). Several inducible promoters (“gene switches”) have been reported, many of which are described in the review by Gatz (1996) and Gatz (1997). These include tetracycline repressor system, Lac repressor system, copper inducible systems, salicylate inducible systems (such as the PR1a system), glucocorticoid (Aoyama et al., 1997) and ecdysone inducible systems. Also included are the benzene sulphonamide (U.S. Pat. No. 5,364,780) and alcohol (WO 97/06269 and WO 97/06268) inducible systems and glutathione S-transferase promoters.

In some instances it might be desirable to inhibit expression of a native DNA sequence within a plant's tissues to achieve a desired phenotype. In this case, such inhibition might be accomplished with transformation of the plant to comprise a constitutive, tissue-independent promoter operably linked to an antisense nucleotide sequence, such that constitutive expression of the antisense sequence produces an RNA transcript that interferes with translation of the mRNA of the native DNA sequence.

Inducible regulatory elements also are useful for purposes of the present invention. As used herein, the term “inducible regulatory element” means a regulatory element that, when exposed to an inducing agent, effects an increased level of transcription of a nucleotide sequence to which it is operatively linked as compared to the level of transcription, if any, in the absence of an inducing agent. Inducible regulatory elements can be those that have no basal or constitutive activity and only effect transcription upon exposure to an inducing agent, or those that effect a basal or constitutive level of transcription, which is increased upon exposure to an inducing agent. Inducible regulatory elements that effect a basal or constitutive level of expression generally are useful in a method or composition of the invention where the induced level of transcription is substantially greater than the basal or constitutive level of expression, for example, at least about two-fold greater, or at least about five-fold greater. Particularly useful inducible regulatory elements do not have a basal or constitutive activity, or increase the level of transcription at least about ten-fold greater than a basal or constitutive level of transcription associated with the regulatory element.

Inducible promoters that have been described include the ABA- and turgor-inducible promoters, the promoter of the auxin-binding protein gene (Schwob et al., 1993), the UDP glucose flavonoid glycosyl-transferase gene promoter (Ralston et al., 1988), the MPI proteinase inhibitor promoter (Cordero et al., 1994), and the glyceraldehyde-3-phosphate dehydrogenase gene promoter (Kohler et al., 1995; Quigley et al., 1989; Martinez et al., 1989).

The term “inducing agent” is used to refer to a chemical, biological or physical agent or environmental condition that effects transcription from an inducible regulatory element. In response to exposure to an inducing agent, transcription from the inducible regulatory element generally is initiated de novo or is increased above a basal or constitutive level of expression. Such induction can be identified using the methods disclosed herein, including detecting an increased level of RNA transcribed from a nucleotide sequence operatively linked to the regulatory element, increased expression of a polypeptide encoded by the nucleotide sequence, or a phenotype conferred by expression of the encoded polypeptide.

An inducing agent useful in a method of the invention is selected based on the particular inducible regulatory element. For example, the inducible regulatory element can be a metallothionein regulatory element, a copper inducible regulatory element or a tetracycline inducible regulatory element, the transcription from which can be effected in response to metal ions, copper or tetracycline, respectively (Furst et al., Cell 55:705–717, 1988; Mett et al., Proc. Natl. Acad. Sci. USA 90:4567–4571, 1993; Gatz et al., Plant J. 2:397–404, 1992; Roder et al., Mol. Gen. Genet. 243:32–38, 1994, each of which is incorporated herein by reference). The inducible regulatory element also can be an ecdysone regulatory element or a glucocorticoid regulatory element, the transcription from which can be effected in response to ecdysone or other steroid (Christopherson et al., Proc. Natl. Acad. Sci., USA 89:6314–6318, 1992; Schena et al., Proc. Natl. Acad. Sci., USA 88:10421–10425, 1991, each of which is incorporated herein by reference). In addition, the regulatory element can be a cold responsive regulatory element or a heat shock regulatory element, the transcription of which can be effected in response to exposure to cold or heat, respectively (Takahashi et al., Plant Physiol. 99:383–390, 1992, which is incorporated herein by reference). Additional regulatory elements useful in the methods or compositions of the invention include, for example, the spinach nitrite reductase gene regulatory element (Back et al., Plant Mol. Biol. 17:9, 1991, which is incorporated herein by reference); a light inducible regulatory element (Feinbaum et al., Mol. Gen. Genet. 226:449, 1991; Lam and Chua, Science 248:471, 1990, each of which is incorporated herein by reference), a plant hormone inducible regulatory element (Yamaguchi-Shinozaki et al., Plant Mol. Biol. 15:905, 1990; Kares et al., Plant Mol. Biol. 15:225, 1990, each of which is incorporated herein by reference), and the like.

An inducible regulatory element also can be a plant stress-regulated regulatory element of the invention. In addition to the known stress conditions that specifically induce or repress expression from such elements, the present invention provides methods of identifying agents that mimic a stress condition. Accordingly, such stress mimics are considered inducing or repressing agents with respect to a plant stress-regulated regulatory element. In addition, a recombinant polypeptide comprising a zinc finger domain, which is specific for the regulatory element, and an effector domain, particularly an activator, can be useful as an inducing agent for a plant stress-regulated regulatory element. Furthermore, such a recombinant polypeptide provides the advantage that the effector domain can be a repressor domain, thereby providing a repressing agent, which decreases expression from the regulatory element. In addition, use of such a method of modulating expression of an endogenous plant stress-regulated gene provides the advantage that the polynucleotide encoding the recombinant polypeptide can be introduced into cells of the plant, thus providing a transgenic plant that can be regulated coordinately with the endogenous plant stress-regulated gene upon exposure to a stress condition. A polynucleotide encoding such a recombinant polypeptide can be operatively linked to and expressed from a constitutively active, inducible or tissue specific or phase specific regulatory element.

In one embodiment, the promoter may be a gamma zein promoter, an oleosin ole16 promoter, a globulin I promoter, an actin I promoter, an actin c1 promoter, a sucrose synthetase promoter, an INOPS promoter, an EXM5 promoter, a globulin2 promoter, a b-32, ADPG-pyrophosphorylase promoter, an LtpI promoter, an Ltp2 promoter, an oleosin ole17 promoter, an oleosin ole18 promoter, an actin 2 promoter, a pollen-specific protein promoter, a pollen-specific pectate lyase promoter, an anther-specific protein promoter (Huffman), an anther-specific gene RTS2 promoter, a pollen-specific gene promoter, a tapeturn-specific gene promoter, tapeturn-specific gene RAB24 promoter, a anthranilate synthase alpha subunit promoter, an alpha zein promoter, an anthranilate synthase beta subunit promoter, a dihydrodipicolinate synthase promoter, a Thi 1 promoter, an alcohol dehydrogenase promoter, a cab binding protein promoter, an H3C4 promoter, a RUBISCO SS starch branching enzyme promoter, an ACCase promoter, an actin3 promoter, an actin7 promoter, a regulatory protein GF14-12 promoter, a ribosomal protein L9 promoter, a cellulose biosynthetic enzyme promoter, an S-adenosyl-L-homocysteine hydrolase promoter, a superoxide dismutase promoter, a C-kinase receptor promoter, a phosphoglycerate mutase promoter, a root-specific RCc3 mRNA promoter, a glucose-6 phosphate isomerase promoter, a pyrophosphate-fructose 6-phosphate-1-phosphotransferase promoter, an ubiquitin promoter, a beta-ketoacyl-ACP synthase promoter, a 33 kDa photosystem 11 promoter, an oxygen evolving protein promoter, a 69 kDa vacuolar ATPase subunit promoter, a metallothionein-like protein promoter, a glyceraldehyde-3-phosphate dehydrogenase promoter, an ABA- and ripening-inducible-like protein promoter, a phenylalanine ammonia lyase promoter, an adenosine triphosphatase S-adenosyl-L-homocysteine hydrolase promoter, an a-tubulin promoter, a cab promoter, a PEPCase promoter, an R gene promoter, a lectin promoter, a light harvesting complex promoter, a heat shock protein promoter, a chalcone synthase promoter, a zein promoter, a globulin-1 promoter, an ABA promoter, an auxin-binding protein promoter, a UDP glucose flavonoid glycosyl-transferase gene promoter, an NTI promoter, an actin promoter, an opaque 2 promoter, a b70 promoter, an oleosin promoter, a CaMV 35S promoter, a CaMV 19S promoter, a histone promoter, a turgor-inducible promoter, a pea small subunit RuBP carboxylase promoter, a Ti plasmid mannopine synthase promoter, Ti plasmid nopaline synthase promoter, a petunia chalcone isomerase promoter, a bean glycine rich protein I promoter, a CaMV 35S transcript promoter, a potato patatin promoter, or a S-E9 small subunit RuBP carboxylase promoter.

In addition to promoters, a variety of 5′ and 3′ transcriptional regulatory sequences are also available for use in the present invention. Transcriptional terminators are responsible for the termination of transcription and correct mRNA polyadenylation. The 3′-untranslated regulatory DNA sequence preferably includes from about 50 to about 1,000, more preferably about 100 to about 1,000, nucleotide base pairs and contains plant transcriptional and translational termination sequences. Appropriate transcriptional terminators and those which are known to function in plants include the CaMV 35S terminator, the tml terminator, the nopaline synthase terminator, the pea rbcS E9 terminator, the terminator for the T7 transcript from the octopine synthase gene of Agrobacterium tumefaciens, and the 3′ end of the protease inhibitor I or II genes from potato or tomato, although other 3′ elements known to those of skill in the art can also be employed. Alternatively, one also could use a gamma coixin, oleosin 3 or other terminator from the genus Coix. Preferred 3′ elements include those from the nopaline synthase gene of Agrobacterium tumefaciens (Bevan et al., 1983), the terminator for the T7 transcript from the octopine synthase gene of Agrobacterium tumefaciens, and the 3′ end of the protease inhibitor I or II genes from potato or tomato.

As the DNA sequence between the transcription initiation site and the start of the coding sequence, i.e., the untranslated leader sequence, can influence gene expression, one may also wish to employ a particular leader sequence. Preferred leader sequences are contemplated to include those that include sequences predicted to direct optimum expression of the attached sequence, i.e., to include a preferred consensus leader sequence that may increase or maintain mRNA stability and prevent inappropriate initiation of translation. The choice of such sequences will be known to those of skill in the art in light of the present disclosure. Sequences that are derived from genes that are highly expressed in plants will be most preferred.

Other sequences that have been found to enhance gene expression in transgenic plants include intron sequences (e.g., from Adh1, bronze1, actin1, actin2 (WO 00/760067), or the sucrose synthase intron) and viral leader sequences (e.g., from TMV, MCMV and AMV). For example, a number of non-translated leader sequences derived from viruses are known to enhance expression. Specifically, leader sequences from tobacco mosaic virus (TMV), maize chlorotic mottle virus (MCMV), and alfalfa mosaic virus (AMV) have been shown to be effective in enhancing expression (e.g., Gallie et al., 1987; Skuzeski et al., 1990). Other leaders known in the art include but are not limited to picornavirus leaders, for example, EMCV leader (encephalomyocarditis virus 5′ non-coding region; Elroy-Stein et al., 1989); potyvirus leaders, for example, TEV leader (tobacco etch virus); MDMV leader (maize dwarf mosaic virus); human immunoglobulin heavy chain binding protein (BiP) leader, (Macejak et al., 1991); untranslated leader from the coat protein mRNA of AMV (AMV RNA 4; Jobling et al., 1987), TMV (Gallie et al., 1989), and MCMV (Lommel et al., 1991; see also, della Cioppa et al., 1987).

Regulatory elements such as Adh intron 1 (Callis et al., 1987), sucrose synthase intron (Vasil et al., 1989) or TMV omega element (Gallie, et al., 1989), may further be included where desired. Examples of enhancers include elements from the CaMV 35S promoter, octopine synthase genes (Ellis et al., 1987), the rice actin I gene, the maize alcohol dehydrogenase gene (Callis et al., 1987), the maize shrunken I gene (Vasil et al., 1989), TMV Omega element (Gallie et al., 1989) and promoters from non-plant eukaryotes (e.g. yeast; Ma et al., 1988).

Vectors for use in accordance with the present invention may be constructed to include the ocs enhancer element, which was first identified as a 16 bp palindromic enhancer from the octopine synthase (ocs) gene of ultilane (Ellis et al., 1987), and is present in at least 10 other promoters (Bouchez et al., 1989). The use of an enhancer element, such as the ocs element and particularly multiple copies of the element, will act to increase the level of transcription from adjacent promoters when applied in the context of monocot transformation.

The methods of the invention provide genetically modified plant cells, which can contain, for example, a coding region, or peptide portion thereof, of a plant stress-regulated gene operatively linked to a heterologous inducible regulatory element; or a plant stress-regulated regulatory element operatively linked to a heterologous nucleotide sequence encoding a polypeptide of interest. In such a plant, the expression from the inducible regulatory element can be effected by exposing the plant cells to an inducing agent in any of numerous ways depending, for example, on the inducible regulatory element and the inducing agent. For example, where the inducible regulatory element is a cold responsive regulatory element present in the cells of a transgenic plant, the plant can be exposed to cold conditions, which can be produced artificially, for example, by placing the plant in a thermostatically controlled room, or naturally, for example, by planting the plant in an environment characterized, at least in part, by attaining temperatures sufficient to induce transcription from the promoter but not so cold as to kill the plants. By examining the phenotype of such transgenic plants, those plants that ectopically express a gene product that confers increased resistance of the plant to cold can be identified. Similarly, a transgenic plant containing a metallothionein promoter can be exposed to metal ions such as cadmium or copper by watering the plants with a solution containing the inducing metal ions, or can be planted in soil that is contaminated with a level of such metal ions that is toxic to most plants. The phenotype of surviving plants can be observed, those expressing desirable traits can be selected.

As used herein, the term “phenotype” refers to a physically detectable characteristic. A phenotype can be identified visually by inspecting the physical appearance of a plant following exposure, for example, to increased osmotic conditions; can be identified using an assay to detecting a product produced due to expression of reporter gene, for example, an RNA molecule, a polypeptide such as an enzyme, or other detectable signal such as disclosed herein; or by using any appropriate tool useful for identifying a phenotype of a plant, for example, a microscope, a fluorescence activated cell sorter, or the like.

A transgenic plant containing an inducible regulatory element such as a steroid inducible regulatory element can be exposed to a steroid by watering the plants with a solution containing the steroid. The use of an inducible regulatory element that is induced upon exposure to a chemical or biological inducing agent that can be placed in solution or suspension in an aqueous medium can be particularly useful because the inducing agent can be applied conveniently to a relatively large crop of transgenic plants containing the inducible regulatory element, for example, through a watering system or by spraying the inducing agent over the field. As such, inducible regulatory elements that are responsive to an environmental inducing agent, for example, cold; heat; metal ions or other potentially toxic agents such as a pesticides, which can contaminate a soil; or the like; or inducible regulatory elements that are regulated by inducing agents that conveniently can be applied to plants, can be particularly useful in a method or composition of the invention, and allow the identification and selection of plants that express desirable traits and survive and grow in environments that otherwise would not support growth of the plants.

As disclosed herein, the present invention provides plant stress-regulated regulatory elements, which are identified based on the expression of clusters of plant genes in response to stress. As used herein, the term “stress-regulated regulatory element of a plant” or “plant stress-regulated regulatory element” means a nucleotide sequence of a plant genome that can respond to a stress such that expression of a gene product encoded by a gene comprising the regulatory element (a stress-inducible gene) is increased above or decreased below the level of expression of the gene product in the absence of the stress condition. The regulatory element can be any gene regulatory element, including, for example, a promoter, an enhancer, a silencer, or the like. In one embodiment, the plant stress-regulated regulatory element is a plant stress-regulated promoter.

For purposes of modulating the responsiveness of a plant to a stress condition, it can be useful to introduce a modified plant stress-regulated regulatory element into a plant. Such a modified regulatory element can have any desirable characteristic, for example, it can be inducible to a greater level than the corresponding wild-type promoter, or it can be inactivated such that, upon exposure to a stress, there is little or no induction of expression of a nucleotide sequence operatively linked to the mutant element. A plant stress-regulated regulatory element can be modified by incorporating random mutations using, for example, in vitro recombination or DNA shuffling (Stemmer et al., Nature 370: 389–391, 1994; U.S. Pat. No. 5,605,793, each of which is incorporated herein by reference). Using such a method, millions of mutant copies of the polynucleotide, for example, stress-regulated regulatory element, can be produced based on the original nucleotide sequence, and variants with improved properties, such as increased inducibility can be recovered.

A mutation method such as DNA shuffling encompasses forming a mutagenized double-stranded polynucleotide from a template double-stranded polynucleotide, wherein the template double-stranded polynucleotide has been cleaved into double stranded random fragments of a desired size, and comprises the steps of adding to the resultant population of double-stranded random fragments one or more single or double stranded oligonucleotides, wherein the oligonucleotides comprise an area of identity and an area of heterology to the double stranded template polynucleotide; denaturing the resultant mixture of double stranded random fragments and oligonucleotides into single stranded fragments; incubating the resultant population of single stranded fragments with a polymerase under conditions that result in the annealing of the single stranded fragments at the areas of identity to form pairs of annealed fragments, the areas of identity being sufficient for one member of a pair to prime replication of the other, thereby forming a mutagenized double-stranded polynucleotide; and repeating the second and third steps for at least two further cycles, wherein the resultant mixture in the second step of a further cycle includes the mutagenized double-stranded polynucleotide from the third step of the previous cycle, and the further cycle forms a further mutagenized double-stranded polynucleotide. Preferably, the concentration of a single species of double stranded random fragment in the population of double stranded random fragments is less than 1% by weight of the total DNA. In addition, the template double stranded polynucleotide can comprise at least about 100 species of polynucleotides. The size of the double stranded random fragments can be from about 5 base pairs to 5 kilobase pairs. In a further embodiment, the fourth step of the method comprises repeating the second and the third steps for at least 10 cycles.

A plant stress-regulated regulatory element of the invention is useful for expressing a nucleotide sequence operatively linked to the element in a cell, particularly a plant cell. As used herein, the term “expression” refers to the transcription and/or translation of an endogenous gene or a transgene in plants. In the case of an antisense molecule, for example, the term “expression” refers to the transcription of the polynucleotide encoding the antisense molecule.

As used herein, the term “operatively linked,” when used in reference to a plant stress-regulated regulatory element, means that the regulatory element is positioned with respect to a second nucleotide sequence such that the regulatory element effects transcription or transcription and translation of the nucleotide sequence in substantially the same manner, but not necessarily to the same extent, as it does when the regulatory element is present in its natural position in a genome. Transcriptional promoters, for example, generally act in a position and orientation dependent manner and usually are positioned at or within about five nucleotides to about fifty nucleotides 5′ (upstream) of the start site of transcription of a gene in nature. In comparison, enhancers and silencers can act in a relatively position or orientation independent manner and, therefore, can be positioned several hundred or thousand nucleotides upstream or downstream from a transcription start site, or in an intron within the coding region of a gene, yet still be operatively linked to a coding region so as to effect transcription.

The second nucleotide sequence, i.e., the sequence operatively linked to the plant stress-regulated regulatory element, can be any nucleotide sequence, including, for example, a coding region of a gene or cDNA; a sequence encoding an antisense molecule, an RNAi molecule, ribozyme, triplexing agent (see, for example, Frank-Kamenetskii and Mirkin, Ann. Rev. Biochem. 64:65–95, 1995), or the like; or a sequence that, when transcribed, can be detected in the cell using, for example, by hybridization or amplification, or when translated produces a detectable signal. The term “coding region” is used broadly herein to include a nucleotide sequence of a genomic DNA or a cDNA molecule comprising all or part of a coding region of the coding strand. A coding region can be transcribed from an operatively linked regulatory element, and can be translated into a full length polypeptide or a peptide portion of a polypeptide. It should be recognized that, in a nucleotide sequence comprising a coding region, not all of the nucleotides in the sequence need necessarily encode the polypeptide and, particularly, that a gene transcript can contain one or more introns, which do not encode an amino acid sequence of a polypeptide but, nevertheless, are part of the coding region, particularly the coding strand, of the gene.

The present invention also relates to a recombinant polynucleotide, which contains a polynucleotide portion of a plant stress-regulated gene operatively linked to a heterologous nucleotide sequence. As used herein, the term “polynucleotide portion of plant stress-regulated sequence” means a contiguous nucleotide sequence of the plant stress-regulated gene that provides a function. The portion can be any portion of the sequence, particularly a coding sequence, or a sequence encoding a peptide portion of the stress-regulated polypeptide; the stress-regulated regulatory element; a sequence useful as an antisense molecule or triplexing agent; or a sequence useful for disrupting (knocking-out) an endogenous plant stress-regulated gene.

A heterologous nucleotide sequence is a nucleotide sequence that is not normally part of the plant stress-regulated gene from which the polynucleotide portion of the plant stress-regulated gene-component of the recombinant polynucleotide is obtained; or, if it is a part of the plant stress-regulated gene from which the polynucleotide portion is obtained, it is an orientation other than it would normally be in, for example, is an antisense sequence, or comprises at least partially discontinuous as compared to the genomic structure, for example, a single exon operatively linked to the regulatory element. In general, where the polynucleotide portion of the plant stress-regulated gene comprises the coding sequence in a recombinant polynucleotide of the invention, the heterologous nucleotide sequence will function as a regulatory element. The regulatory element can be any heterologous regulatory element, including, for example, a constitutively active regulatory element, an inducible regulatory element, or a tissue specific or phase specific regulatory element, as disclosed above. Conversely, where the polynucleotide portion of the plant stress-regulated polynucleotide comprises the stress-regulated regulatory element of a recombinant polynucleotide of the invention, the heterologous nucleotide sequence generally will be a nucleotide sequence that can be transcribed and, if desired, translated. Where the heterologous nucleotide sequence is expressed from a plant stress-regulated regulatory element, it generally confers a desirable phenotype to a plant cell containing the recombinant polynucleotide, or provides a means to identify a plant cell containing the recombinant polynucleotide. It should be recognized that a “desirable” phenotype can be one that decreases the ability of a plant cell to compete where the plant cell, or a plant containing the cell, is an undesired plant cell. Thus, a heterologous nucleotide sequence can allow a plant to grow, for example, under conditions in which it would not normally be able to grow.

A heterologous nucleotide sequence can be, or encode, a selectable marker. As used herein, the term “selectable marker” is used herein to refer to a molecule that, when present or expressed in a plant cell, provides a means to identify a plant cell containing the marker. As such, a selectable marker can provide a means for screening a population of plants, or plant cells, to identify those having the marker. A selectable marker also can confer a selective advantage to the plant cell, or a plant containing the cell. The selective advantage can be, for example, the ability to grow in the presence of a negative selective agent such as an antibiotic or herbicide, compared to the growth of plant cells that do not contain the selectable marker. The selective advantage also can be due, for example, to an enhanced or novel capacity to utilize an added compound as a nutrient, growth factor or energy source. A selectable advantage can be conferred, for example, by a single polynucleotide, or its expression product, or to a combination of polynucleotides whose expression in a plant cell gives the cell with a positive selective advantage, a negative selective advantage, or both.

Examples of selectable markers include those that confer antimetabolite resistance, for example, dihydrofolate reductase, which confers resistance to methotrexate (Reiss, Plant Physiol. (Life Sci. Adv.) 13:143–149, 1994); neomycin phosphotransferase, which confers resistance to the aminoglycosides neomycin, kanamycin and paromycin (Herrera-Estrella, EMBO J. 2:987–995, 1983) and hygro, which confers resistance to hygromycin (Marsh, Gene 32:481–485, 1984), trpB, which allows cells to utilize indole in place of tryptophan; hisD, which allows cells to utilize histinol in place of histidine (Hartman, Proc. Natl. Acad. Sci., USA 85:8047, 1988); mannose-6-phosphate isomerase which allows cells to utilize mannose (WO 94/20627); ornithine decarboxylase, which confers resistance to the ornithine decarboxylase inhibitor, 2-(difluoromethyl)-DL-ornithine (DFMO; McConlogue, 1987, In: Current Communications in Molecular Biology, Cold Spring Harbor Laboratory ed.); and deaminase from Aspergillus terreus, which confers resistance to Blasticidin S (Tamura, Biosci. Biotechnol. Biochem. 59:2336–2338, 1995). Additional selectable markers include those that confer herbicide resistance, for example, phosphinothricin acetyltransferase gene, which confers resistance to phosphinothricin (White et al., Nucl. Acids Res. 18:1062, 1990; Spencer et al., Theor. Appl. Genet. 79:625–631, 1990), a mutant EPSPV-synthase, which confers glyphosate resistance (Hinchee et al., Bio/Technology 91:915–922, 1998), a mutant acetolactate synthase, which confers imidazolione or sulfonylurea resistance (Lee et al., EMBO J. 7:1241–1248, 1988), a mutant psbA, which confers resistance to atrazine (Smeda et al., Plant Physiol. 103:911–917, 1993), or a mutant protoporphyrinogen oxidase (see U.S. Pat. No. 5,767,373), or other markers conferring resistance to an herbicide such as glufosinate. In addition, markers that facilitate identification of a plant cell containing the polynucleotide encoding the marker include, for example, luciferase (Giacomin, Plant Sci. 116:59–72, 1996; Scikantha, J. Bacteriol. 178:121, 1996), green fluorescent protein (Gerdes, FEBS Lett. 389:44–47, 1996) or fl-glucuronidase (Jefferson, EMBO J. 6:3901–3907, 1997), and numerous others as disclosed herein or otherwise known in the art. Such markers also can be used as reporter molecules.

A heterologous nucleotide sequence can encode an antisense molecule, particularly an antisense molecule specific for a nucleotide sequence of a plant stress-regulated gene, for example, the gene from which the regulatory component of the recombinant polynucleotide is derived. Such a recombinant polynucleotide can be useful for reducing the expression of a plant stress-regulated polypeptide in response to a stress condition because the antisense molecule, like the polypeptide, only will be induced upon exposure to the stress. A heterologous nucleotide sequence also can be, or can encode, a ribozyme or a triplexing agent. In addition to being useful as heterologous nucleotide sequences, such molecules also can be used directly in a method of the invention, for example, to modulate the responsiveness of a plant cell to a stress condition. Thus, an antisense molecule, ribozyme, or triplexing agent can be contacted directly with a target cell and, upon uptake by the cell, can effect their antisense, ribozyme or triplexing activity; or can be encoded by a heterologous nucleotide sequence that is expressed in a plant cell from a plant stress-regulated regulatory element, whereupon it can effect its activity.

An antisense polynucleotide, ribozyme or triplexing agent is complementary to a target sequence, which can be a DNA or RNA sequence, for example, messenger RNA, and can be a coding sequence, a nucleotide sequence comprising an intron-exon junction, a regulatory sequence such as a Shine-Delgarno-like sequence, or the like. The degree of complementarity is such that the polynucleotide, for example, an antisense polynucleotide, can interact specifically with the target sequence in a cell. Depending on the total length of the antisense or other polynucleotide, one or a few mismatches with respect to the target sequence can be tolerated without losing the specificity of the polynucleotide for its target sequence. Thus, few if any mismatches would be tolerated in an antisense molecule consisting, for example, of twenty nucleotides, whereas several mismatches will not affect the hybridization efficiency of an antisense molecule that is complementary, for example, to the full length of a target mRNA encoding a cellular polypeptide. The number of mismatches that can be tolerated can be estimated, for example, using well known formulas for determining hybridization kinetics (see Sambrook et al., “Molecular Cloning; A Laboratory Manual” 2nd Edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; 1989)) or can be determined empirically using methods as disclosed herein or otherwise known in the art, particularly by determining that the presence of the antisense polynucleotide, ribozyme, or triplexing agent in a cell decreases the level of the target sequence or the expression of a polypeptide encoded by the target sequence in the cell.

A nucleotide sequence useful as an antisense molecule, a ribozyme or a triplexing agent can inhibit translation or cleave a polynucleotide encoded by plant stress-regulated gene, thereby modulating the responsiveness of a plant cell to a stress condition. An antisense molecule, for example, can bind to an mRNA to form a double stranded molecule that cannot be translated in a cell. Antisense oligonucleotides of at least about 15 to 25 nucleotides are preferred since they are easily synthesized and can hybridize specifically with a target sequence, although longer antisense molecules can be expressed from a recombinant polynucleotide introduced into the target cell. Specific nucleotide sequences useful as antisense molecules can be identified using well known methods, for example, gene walking methods (see, for example, Seimiya et al., J. Biol. Chem. 272:4631–4636 (1997), which is incorporated herein by reference). Where the antisense molecule is contacted directly with a target cell, it can be operatively associated with a chemically reactive group such as iron-linked EDTA, which cleaves a target RNA at the site of hybridization. A triplexing agent, in comparison, can stall transcription (Maher et al., Antisense Res. Devel. 1:227 (1991); Helene, Anticancer Drug Design 6:569 (1991)).

A plant stress-regulated regulatory element can be included in an expression cassette. As used herein, the term “expression cassette” refers to a nucleotide sequence that can direct expression of an operatively linked polynucleotide. Thus, a plant stress-regulated regulatory element can constitute an expression cassette, or component thereof. An expression cassette is particularly useful for directing expression of a nucleotide sequence, which can be an endogenous nucleotide sequence or a heterologous nucleotide sequence, in a cell, particularly a plant cell. If desired, an expression cassette also can contain additional regulatory elements, for example, nucleotide sequences required for proper translation of a polynucleotide sequence into a polypeptide. In general, an expression cassette can be introduced into a plant cell such that the plant cell, a plant resulting from the plant cell, seeds obtained from such a plant, or plants produced from such seeds are resistant to a stress condition.

Additional regulatory sequences as disclosed above or other desirable sequences such as selectable markers or the like can be incorporated into an expression cassette containing a plant stress-regulated regulatory element (see, for example, WO 99/47552). Examples of suitable markers include dihydrofolate reductase (DHFR) or neomycin resistance for eukaryotic cells and tetracycline or ampicillin resistance for E. coli. Selection markers in plants include bleomycin, gentamycin, glyphosate, hygromycin, kanamycin, methotrexate, phleomycin, phosphinotricin, spectinomycin, streptomycin, sulfonamide and sulfonylureas resistance (see, for example, Maliga et al., Methods in Plant Molecular Biology, Cold Spring Harbor Laboratory Press, 1995, page 39). The selection marker can have its own promoter or its expression can be driven by the promoter operably linked to the sequence of interest. Additional sequences such as intron sequences (e.g. from Adh1 or bronze1) or viral leader sequences (e.g. from TMV, MCMV and AIVIV), all of which can enhance expression, can be included in the cassette. In addition, where it is desirable to target expression of a nucleotide sequence operatively linked to the stress-regulated regulatory element, a sequence encoding a cellular localization motif can be included in the cassette, for example, such that an encoded transcript or translation product is translocated to and localizes in the cytosol, nucleus, a chloroplast, or another subcellular organelle. Examples of useful transit peptides and transit peptide sequences can be found in Von Heijne et al., Plant Mol. Biol. Rep. 9: 104, 1991; Clark et al., J. Biol. Chem. 264:17544, 1989; della Cioppa et al., Plant Physiol. 84:965, 1987; Romer et al., Biochem. Biophys. Res. Comm. 196:1414, 1993; Shah et al., Science 233:478, 1986; Archer et al., J. Bioenerg Biomemb. 22:789, 1990; Scandalios, Prog. Clin. Biol. Res. 344:515, 1990; Weisbeek et al., J. Cell Sci. Suppl. 11: 199, 1989; Bruce, Trends Cell Biol. 10:440, 2000. The present invention can utilize native or heterologous transit peptides. The encoding sequence for a transit peptide can include all or a portion of the encoding sequence for a particular transit peptide, and may also contain portions of the mature protein encoding sequence associated with a particular transit peptide.

A polynucleotide portion of a plant stress-regulated plant gene, or an expression cassette, can be introduced into a cell as a naked DNA molecule, can be incorporated in a matrix such as a liposome or a particle such as a viral particle, or can be incorporated into a vector. Such vectors can be cloning or expression vectors, but other uses are within the scope of the present invention. A cloning vector is a self-replicating DNA molecule that serves to transfer a DNA segment into a host cell. The three most common types of cloning vectors are bacterial plasmids, phages, and other viruses. An expression vector is a cloning vector designed so that a coding sequence inserted at a particular site will be transcribed and translated into a protein. Incorporation of the polynucleotide into a vector can facilitate manipulation of the polynucleotide, or introduction of the polynucleotide into a plant cell. A vector can be derived from a plasmid or a viral vector such as a T-DNA vector (Horsch et al., Science 227:1229–1231, 1985, which is incorporated herein by reference). If desired, the vector can comprise components of a plant transposable element, for example, a Ds transposon (Bancroft and Dean, Genetics 134:1221–1229, 1993, which is incorporated herein by reference) or an Spm transposon (Aarts et al., Mol. Gen. Genet. 247:555–564, 1995, which is incorporated herein by reference).

In addition to containing the polynucleotide portion of a plant stress-regulated gene, a vector can contain various nucleotide sequences that facilitate, for example, rescue of the vector from a transformed plant cell; passage of the vector in a host cell, which can be a plant, animal, bacterial, or insect host cell; or expression of an encoding nucleotide sequence in the vector, including all or a portion of a rescued coding region. As such, the vector can contain any of a number of additional transcription and translation elements, including constitutive and inducible promoters, enhancers, and the like (see, for example, Bitter et al., Meth. Enzymol. 153:516–544, 1987). For example, a vector can contain elements useful for passage, growth or expression in a bacterial system, including a bacterial origin of replication; a promoter, which can be an inducible promoter; and the like. In comparison, a vector that can be passaged in a mammalian host cell system can have a promoter such as a metallothionein promoter, which has characteristics of both a constitutive promoter and an inducible promoter, or a viral promoter such as a retrovirus long terminal repeat, an adenovirus late promoter, or the like. A vector also can contain one or more restriction endonuclease recognition and cleavage sites, including, for example, a polylinker sequence, to facilitate rescue of a nucleotide sequence operably linked to the polynucleotide portion.

The present invention also relates to a method of using a polynucleotide portion of a plant stress-regulated gene to confer a selective advantage on a plant cell. Such a method can be performed by introducing, for example, a plant stress-regulated regulatory element into a plant cell, wherein, upon exposure of the plant cell to a stress condition to which the regulatory element is responsive, a nucleotide sequence operatively linked to the regulatory element is expressed, thereby conferring a selective advantage to plant cell. The operatively linked nucleotide sequence can be a heterologous nucleotide sequence, which can be operatively linked to the regulatory element prior to introduction of the regulatory sequence into the plant cell; or can be an endogenous nucleotide sequence into which the regulatory element was targeted by a method such as homologous recombination. The selective advantage conferred by the operatively linked nucleotide sequence can be such that the plant is better able to tolerate the stress condition; or can be any other selective advantage.

As used herein, the term “selective advantage” refers to the ability of a particular organism to better propagate, develop, grow, survive, or otherwise tolerate a condition as compared to a corresponding reference organism that does not contain a plant-stress regulated polynucleotide portion of the present invention. In one embodiment, a selective advantage is exemplified by the ability of a desired plant, plant cell, or the like, that contains an introduced plant stress-regulated regulatory element, to grow better than an undesired plant, plant cell, or the like, that does not contain the introduced regulatory element. For example, a recombinant polynucleotide comprising a plant stress-regulated regulatory element operatively linked to a heterologous nucleotide sequence encoding an enzyme that inactivates an herbicide can be introduced in a desired plant. Upon exposure of a mixed population of plants comprising the desired plants, which contain the recombinant polynucleotide, and one or more other populations of undesired plants, which lack the recombinant polynucleotide, to a stress condition that induces expression of the regulatory element and to the herbicide, the desired plants will have a greater likelihood of surviving exposure to the toxin and, therefore, a selective advantage over the undesired plants.

In another embodiment, a selective advantage is exemplified by the ability of a desired plant, plant cell, or the like, to better propagate, develop, grow, survive, or otherwise tolerate a condition as compared to an undesired plant, plant cell, or the like, that contains an introduced plant stress-regulated regulatory element. For example, a recombinant polynucleotide comprising a plant stress-regulated regulatory element operatively linked to a plant cell toxin can be introduced into cells of an undesirable plant present in a mixed population of desired and undesired plants, for example, food crops and weeds, respectively, then the plants can be exposed to stress conditions that induce expression from the plant stress-regulated regulatory element, whereby expression of the plant cell toxin results in inhibition of growth or death of the undesired plants, thereby providing a selective advantage to the desired plants, which no longer have to compete with the undesired plants for nutrients, light, or the like. In another example, a plant stress-regulated regulatory element operatively linked to a plant cell toxin can be introduced into cells of plants used as a nurse crop. Nurse crops, also called cover or companion crops, are planted in combination with plants of interest to provide, among other things, shade and soil stability during establishment of the desired plants. Once the desired plants have become established, the presence of the nurse crop may no longer be desirable. Exposure to conditions inducing expression of the gene linked to the plant stress-regulated regulatory element allows elimination of the nurse crop. Alternatively nurse crops can be made less tolerate to abiotic stress by the inhibition of any of the stress-regulated sequences disclosed herein. Inhibition can be accomplished by any of the method described herein. Upon exposure of the nurse crop to the stress, the decreased ability of the nurse crop to respond to the stress will result in elimination of the nurse crop, leaving only the desired plants.

The invention also provides a means of producing a transgenic plant, which comprises plant cells that exhibit altered responsiveness to a stress condition. As such, the present invention further provides a transgenic plant, or plant cells or tissues derived therefrom, which are genetically modified to respond to stress differently than a corresponding wild-type plant or plant not containing constructs of the present invention would respond. As used herein, the term “responsiveness to a stress condition” refers to the ability of a plant to express a plant stress-regulated gene upon exposure to the stress condition. A transgenic plant cell contains a polypeptide portion of a plant stress-regulated gene, or a mutant form thereof, for example, a knock-out mutant. A knock-out mutant form of a plant stress-regulated gene can contain, for example, a mutation such that a STOP codon is introduced into the reading frame of the translated portion of the gene such that expression of a functional stress-regulated polypeptide is prevented; or a mutation in the stress-regulated regulatory element such that inducibility of the element in response to a stress condition is inhibited. Such transgenic plants of the invention can display any of various idiotypic modifications is response to an abiotic stress, including altered tolerance to the stress condition, as well as increased or decreased plant growth, root growth, yield, or the like, as compared to the corresponding wild-type plant.

The term “plant” is used broadly herein to include any plant at any stage of development, or to part of a plant, including a plant cutting, a plant cell, a plant cell culture, a plant organ, a plant seed, and a plantlet. A plant cell is the structural and physiological unit of the plant, comprising a protoplast and a cell wall. A plant cell can be in the form of an isolated single cell or a cultured cell, or can be part of higher organized unit, for example, a plant tissue, plant organ, or plant. Thus, a plant cell can be a protoplast, a gamete producing cell, or a cell or collection of cells that can regenerate into a whole plant. As such, a seed, which comprises multiple plant cells and is capable of regenerating into a whole plant, is considered plant cell for purposes of this disclosure. A plant tissue or plant organ can be a seed, protoplast, callus, or any other groups of plant cells that is organized into a structural or functional unit. Particularly useful parts of a plant include harvestable parts and parts useful for propagation of progeny plants. A harvestable part of a plant can be any useful part of a plant, for example, flowers, pollen, seedlings, tubers, leaves, stems, fruit, seeds, roots, and the like. A part of a plant useful for propagation includes, for example, seeds, fruits, cuttings, seedlings, tubers, rootstocks, and the like.

A transgenic plant can be regenerated from a transformed plant cell. As used herein, the term “regenerate” means growing a whole plant from a plant cell; a group of plant cells; a protoplast; a seed; or a piece of a plant such as a callus or tissue. Regeneration from protoplasts varies from species to species of plants. For example, a suspension of protoplasts can be made and, in certain species, embryo formation can be induced from the protoplast suspension, to the stage of ripening and germination. The culture media generally contains various components necessary for growth and regeneration, including, for example, hormones such as auxins and cytokinins; and amino acids such as glutamic acid and proline, depending on the particular plant species. Efficient regeneration will depend, in part, on the medium, the genotype, and the history of the culture. If these variables are controlled, however, regeneration is reproducible.

Regeneration can occur from plant callus, explants, organs or plant parts. Transformation can be performed in the context of organ or plant part regeneration. (see Meth. Enzymol. Vol. 118; Klee et al. Ann. Rev. Plant Physiol. 38:467, 1987, which is incorporated herein by reference). Utilizing the leaf disk-transformation-regeneration method, for example, disks are cultured on selective media, followed by shoot formation in about two to four weeks (see Horsch et al., supra, 1985). Shoots that develop are excised from calli and transplanted to appropriate root-inducing selective medium. Rooted plantlets are transplanted to soil as soon as possible after roots appear. The plantlets can be repotted as required, until reaching maturity.

In vegetatively propagated crops, the mature transgenic plants are propagated utilizing cuttings or tissue culture techniques to produce multiple identical plants. Selection of desirable transgenotes is made and new varieties are obtained and propagated vegetatively for commercial use. In seed propagated crops, the mature transgenic plants can be self crossed to produce a homozygous inbred plant. The resulting inbred plant produces seeds that contain the introduced plant stress-induced regulatory element, and can be grown to produce plants that express a polynucleotide or polypeptide in response to a stress condition that induces expression from the regulatory element. As such, the invention further provides seeds produced by a transgenic plant obtained by a method of the invention.

In addition, transgenic plants comprising different recombinant sequences can be crossbred, thereby providing a means to obtain transgenic plants containing two or more different transgenes, each of which contributes a desirable characteristic to the plant. Methods for breeding plants and selecting for crossbred plants having desirable characteristics or other characteristics of interest are well known in the art.

A method of the invention can be performed by introducing a polynucleotide portion of a plant stress-regulated gene into the plant. As used herein, the term “introducing” means transferring a polynucleotide into a plant cell. A polynucleotide can be introduced into a cell by a variety of methods well known to those of ordinary skill in the art. For example, the polynucleotide can be introduced into a plant cell using a direct gene transfer method such as electroporation or microprojectile mediated transformation, or using Agrobacterium mediated transformation. Non-limiting examples of methods for the introduction of polynucleotides into plants are provided in greater detail herein. As used herein, the term “transformed” refers to a plant cell containing an exogenously introduced polynucleotide portion of a plant stress-regulated gene that is or can be rendered active in a plant cell, or to a plant comprising a plant cell containing such a polynucleotide.

It should be recognized that one or more polynucleotides, which are the same or different can be introduced into a plant, thereby providing a means to obtain a genetically modified plant containing multiple copies of a single transgenic sequence, or containing two or more different transgenic sequences, either or both of which can be present in multiple copies. Such transgenic plants can be produced, for example, by simply selecting plants having multiple copies of a single type of transgenic sequence; by cotransfecting plant cells with two or more populations of different transgenic sequences and identifying those containing the two or more different transgenic sequences; or by crossbreeding transgenic plants, each of which contains one or more desired transgenic sequences, and identifying those progeny having the desired sequences.

Methods for introducing a polynucleotide into a plant cell to obtain a transformed plant also include direct gene transfer (see European Patent A 164 575), injection, electroporation, biolistic methods such as particle bombardment, pollen-mediated transformation, plant RNA virus-mediated transformation, liposome-mediated transformation, transformation using wounded or enzyme-degraded immature embryos, or wounded or enzyme-degraded embryogenic callus, and the like. Transformation methods using Agrobacterium tumefaciens tumor inducing (Ti) plasmids or root-inducing (Ri) plasmids, or plant virus vectors are well known in the art (see, for example, WO 99/47552; Weissbach & Weissbach, “Methods for Plant Molecular Biology” (Academic Press, NY 1988), section VIII, pages 421–463; Grierson and Corey, “Plant Molecular Bioloy” 2d Ed. (Blackie, London 1988), Chapters 7–9, each of which is incorporated herein by reference; Horsch et al., supra, 1985). The wild-type form of Agrobacterium, for example, contains a Ti plasmid, which directs production of tumorigenic crown gall growth on host plants. Transfer of the tumor inducing T-DNA region of the Ti plasmid to a plant genome requires the Ti plasmid-encoded virulence genes as well as T-DNA borders, which are a set of direct DNA repeats that delineate the region to be transferred. An Agrobacterium based vector is a modified form of a Ti plasmid, in which the tumor inducing functions are replaced by a nucleotide sequence of interest that is to be introduced into the plant host.

Methods of using Agrobacterium mediated transformation include cocultivation of Agrobacterium with cultured isolated protoplasts; transformation of plant cells or tissues with Agrobacterium; and transformation of seeds, apices or meristems with Agrobacterium. In addition, in planta transformation by Agrobacterium can be performed using vacuum infiltration of a suspension of Agrobacterium cells (Bechtold et al., C. R. Acad. Sci. Paris 316:1194, 1993, which is incorporated herein by reference).

Agrobacterium mediated transformation can employ cointegrate vectors or binary vector systems, in which the components of the Ti plasmid are divided between a helper vector, which resides permanently in the Agrobacterium host and carries the virulence genes, and a shuttle vector, which contains the gene of interest bounded by T-DNA sequences. Binary vectors are well known in the art (see, for example, De Framond, BioTechnology 1:262, 1983; Hoekema et al., Nature 303:179, 1983, each of which is incorporated herein by reference) and are commercially available (Clontech; Palo Alto Calif.). For transformation, Agrobacterium can be cocultured, for example, with plant cells or wounded tissue such as leaf tissue, root explants, hypocotyledons, stem pieces or tubers (see, for example, Glick and Thompson, “Methods in Plant Molecular Biology and Biotechnology” (Boca Raton Fla., CRC Press 1993), which is incorporated herein by reference). Wounded cells within the plant tissue that have been infected by Agrobacterium can develop organs de novo when cultured under the appropriate conditions; the resulting transgenic shoots eventually give rise to transgenic plants, which contain an exogenous polynucleotide portion of a plant stress-regulated gene.

Agrobacterium mediated transformation has been used to produce a variety of transgenic plants, including, for example, transgenic cruciferous plants such as Arabidopsis, mustard, rapeseed and flax; transgenic leguminous plants such as alfalfa, pea, soybean, trefoil and white clover; and transgenic solanaceous plants such as eggplant, petunia, potato, tobacco and tomato (see, for example, Wang et al., “Transformation of Plants and Soil Microorganisms” (Cambridge, University Press 1995), which is incorporated herein by reference). In addition, Agrobacterium mediated transformation can be used to introduce an exogenous polynucleotide sequence, for example, a plant stress-regulated regulatory element into apple, aspen, belladonna, black currant, carrot, celery, cotton, cucumber, grape, horseradish, lettuce, morning glory, muskmelon, neem, poplar, strawberry, sugar beet, sunflower, walnut, asparagus, rice and other plants (see, for example, Glick and Thompson, supra, 1993; Hiei et al., Plant J. 6:271–282, 1994; Shimamoto, Science 270:1772–1773, 1995).

Suitable strains of Agrobacterium tumefaciens and vectors as well as transformation of Agrobacteria and appropriate growth and selection media are well known in the art (GV3101, pMK90RK), Koncz, Mol. Gen. Genet. 204:383–396, 1986; (C58C1, pGV3850kan), Deblaere, Nucl. Acid Res. 13:4777, 1985; Bevan, Nucl. Acid Res. 12:8711, 1984; Koncz, Proc. Natl. Acad. Sci. USA 86:8467–8471, 1986; Koncz, Plant Mol. Biol. 20:963–976, 1992; Koncz, Specialized vectors for gene tagging and expression studies. In: Plant Molecular Biology Manual Vol. 2, Gelvin and Schilperoort (Eds.), Dordrecht, The Netherlands: Kluwer Academic Publ. (1994), 1–22; European Patent A-1 20 516; Hoekema: The Binary Plant Vector System, Offsetdrukkerij Kanters B. V., Alblasserdam (1985), Chapter V; Fraley, Crit. Rev. Plant. Sci., 4:1–46; An, EMBO J. 4:277–287, 1985).

Where a polynucleotide portion of a plant stress-regulated gene is contained in vector, the vector can contain functional elements, for example “left border” and “right border” sequences of the T-DNA of Agrobacterium, which allow for stable integration into a plant genome. Furthermore, methods and vectors that permit the generation of marker-free transgenic plants, for example, where a selectable marker gene is lost at a certain stage of plant development or plant breeding, are known, and include, for example, methods of co-transformation (Lyznik, Plant Mol. Biol. 13:151–161, 1989; Peng, Plant Mol. Biol. 27:91–104, 1995), or methods that utilize enzymes capable of promoting homologous recombination in plants (see, e.g., WO97/08331; Bayley, Plant Mol. Biol. 18:353–361, 1992; Lloyd, Mol. Gen. Genet. 242:653–657, 1994; Maeser, Mol. Gen. Genet. 230:170–176, 1991; Onouchi, Nucl. Acids Res. 19:6373–6378, 1991; see, also, Sambrook et al., supra, 1989).

A direct gene transfer method such as electroporation also can be used to introduce a polynucleotide portion of a plant stress-regulated gene into a cell such as a plant cell. For example, plant protoplasts can be electroporated in the presence of the regulatory element, which can be in a vector (Fromm et al., Proc. Natl. Acad. Sci., USA 82:5824, 1985, which is incorporated herein by reference). Electrical impulses of high field strength reversibly permeabilize membranes allowing the introduction of the nucleic acid. Electroporated plant protoplasts reform the cell wall, divide and form a plant callus. Microinjection can be performed as described in Potrykus and Spangenberg (eds.), Gene Transfer To Plants (Springer Verlag, Berlin, N.Y. 1995). A transformed plant cell containing the introduced polynucleotide can be identified by detecting a phenotype due to the introduced polynucleotide, for example, increased or decreased tolerance to a stress condition.

Microprojectile mediated transformation also can be used to introduce a polynucleotide into a plant cell (Klein et al., Nature 327:70–73, 1987, which is incorporated herein by reference). This method utilizes microprojectiles such as gold or tungsten, which are coated with the desired nucleic acid molecule by precipitation with calcium chloride, spermidine or polyethylene glycol. The microprojectile particles are accelerated at high speed into a plant tissue using a device such as the BIOLISTIC PD-1000 (BioRad; Hercules Calif.).

Microprojectile mediated delivery (“particle bombardment”) is especially useful to transform plant cells that are difficult to transform or regenerate using other methods. Methods for the transformation using biolistic methods are well known (Wan, Plant Physiol. 104:37–48, 1984; Vasil, Bio/Technology 11:1553–1558, 1993; Christou, Trends in Plant Science 1:423–431, 1996). Microprojectile mediated transformation has been used, for example, to generate a variety of transgenic plant species, including cotton, tobacco, corn, hybrid poplar and papaya (see Glick and Thompson, supra, 1993). Important cereal crops such as wheat, oat, barley, sorghum and rice also have been transformed using microprojectile mediated delivery (Duan et al., Nature Biotech. 14:494–498, 1996; Shimamoto, Curr. Opin. Biotech. 5:158–162, 1994). A rapid transformation regeneration system for the production of transgenic plants such as a system that produces transgenic wheat in two to three months (see European Patent No. EP 0709462A2, which is incorporated herein by reference) also can be useful for producing a transgenic plant using a method of the invention, thus allowing more rapid identification of gene functions. The transformation of most dicotyledonous plants is possible with the methods described above. Transformation of monocotyledonous plants also can be transformed using, for example, biolistic methods as described above, protoplast transformation, electroporation of partially permeabilized cells, introduction of DNA using glass fibers, Agrobacterium mediated transformation, and the like.

Plastid transformation also can be used to introduce a polynucleotide portion of a plant stress-regulated gene into a plant cell (U.S. Pat. Nos. 5,451,513, 5,545,817, and 5,545,818; WO 95/16783; McBride et al., Proc. Natl. Acad. Sci., USA 91:7301–7305, 1994). Chloroplast transformation involves introducing regions of cloned plastid DNA flanking a desired nucleotide sequence, for example, a selectable marker together with polynucleotide of interest into a suitable target tissue, using, for example, a biolistic or protoplast transformation method (e.g., calcium chloride or PEG mediated transformation). One to 1.5 kb flanking regions (“targeting sequences”) facilitate homologous recombination with the plastid genome, and allow the replacement or modification of specific regions of the plastome. Using this method, point mutations in the chloroplast 16S rRNA and rps12 genes, which confer resistance to spectinomycin and streptomycin, can be utilized as selectable markers for transformation (Svab et al., Proc. Natl. Acad. Sci., USA 87:8526–8530, 1990; Staub and Maliga, Plant Cell 4:39–45, 1992), resulted in stable homopiasmic transformants; at a frequency of approximately one per 100 bombardments of target leaves. The presence of cloning sites between these markers allowed creation of a plastid targeting vector for introduction of foreign genes (Staub and Maliga, EMBO J. 12:601–606, 1993). Substantial increases in transformation frequency are obtained by replacement of the recessive rRNA or r-protein antibiotic resistance genes with a dominant selectable marker, the bacterial aadA gene encoding the spectinomycin-detoxifying enzyme aminoglycoside-3′-adenyltransferase (Svab and Maliga, Proc. Natl. Acad. Sci., USA 90:913–917, 1993). Approximately 15 to 20 cell division cycles following transformation are generally required to reach a homoplastidic state. Plastid expression, in which genes are inserted by homologous recombination into all of the several thousand copies of the circular plastid genome present in each plant cell, takes advantage of the enormous copy number advantage over nuclear-expressed genes to permit expression levels that can readily exceed 10% of the total soluble plant protein.

Plants suitable to treatment according to a method of the invention can be monocots or dicots and include, but are not limited to, corn (Zea mays), Brassica sp. (e.g., B. napus, B. rapa, B. juncea), particularly those Brassica species useful as sources of seed oil, alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), millet (e.g., pearl millet (Pennisetum glaucum), proso millet (Panicum miliaceum), foxtail millet (Setaria italica), finger millet (Eleusine coracana)), sunflower (Helianthus annuus), safflower (Carthamus tinctorius), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium barbadense, Gossypium hirsutum), sweet potato (Ipomoea batatus), cassava (Manihot esculenta), coffee (Cofea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea ultilane), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), sugarcane (Saccharum spp.), oats, duckweed (Lemna), barley, tomatoes (Lycopersicon esculentum), lettuce (e.g., Lactuca sativa), green beans (Phaseolus vulgaris), lima beans (Phaseolus limensis), peas (Lathyrus spp.), and members of the genus Cucumis such as cucumber (C. sativus), cantaloupe (C. cantalupensis), and musk melon (C. melo).

Ornamentals such as azalea (Rhododendron spp.), hydrangea (Macrophylla hydrangea), hibiscus (Hibiscus rosasanensis), roses (Rosa spp.), tulips (Tulipa spp.), daffodils (Narcissus spp.), petunias (Petunia hybrida), carnation (Dianthus caryophyllus), poinsettia (Euphorbia pulcherrima), and chrysanthemum are also included. Additional ornamentals within the scope of the invention include impatiens, Begonia, Pelargonium, Viola, Cyclamen, Verbena, Vinca, Tagetes, Primula, Saint Paulia, Agertum, Amaranthus, Antihirrhinum, Aquilegia, Cineraria, Clover, Cosmo, Cowpea, Dahlia, Datura, Delphinium, Gerbera, Gladiolus, Gloxinia, Hippeastrum, Mesembryanthemum, Salpiglossos, and Zinnia.

Conifers that may be employed in practicing the present invention include, for example, pines such as loblolly pine (Pinus taeda), slash pine (Pinus elliotii), ponderosa pine (Pinus ponderosa), lodgepole pine (Pinus contorta), and Monterey pine (Pinus radiata), Douglas-fir (Pseudotsuga menziesii); Western hemlock (Tsuga ultilane); Sitka spruce (Picea glauca); redwood (Sequoia sempervirens); true firs such as silver fir (Abies amabilis) and balsam fir (Abies balsamea); and cedars such as Western red cedar (Thuja plicata) and Alaska yellow-cedar (Chamaecyparis nootkatensis).

Leguminous plants which may be used in the practice of the present invention include beans and peas. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mungbean, lima bean, fava bean, lentils, chickpea, etc. Legumes include, but are not limited to, Arachis, e.g., peanuts, Vicia, e.g., crown vetch, hairy vetch, adzuki bean, mung bean, and chickpea, Lupinus, e.g., lupine, trifolium, Phaseolus, e.g., common bean and lima bean, Pisum, e.g., field bean, Melilotus, e.g., clover, Medicago, e.g., alfalfa, Lotus, e.g., trefoil, lens, e.g., lentil, and false indigo. Preferred forage and turf grass for use in the methods of the invention include alfalfa, orchard grass, tall fescue, perennial ryegrass, creeping bent grass, and redtop.

Angiosperms are divided into two broad classes based on the number of cotyledons, which are seed leaves that generally store or absorb food; a monocotyledonous angiosperm has a single cotyledon, and a dicotyledonous angiosperm has two cotyledons. Angiosperms produce a variety of useful products including materials such as lumber, rubber, and paper; fibers such as cotton and linen; herbs and medicines such as quinine and vinblastine; ornamental flowers such as roses and orchids; and foodstuffs such as grains, oils, fruits and vegetables.

Angiosperms encompass a variety of flowering plants, including, for example, cereal plants, leguminous plants, oilseed plants, hardwood trees, fruit-bearing plants and ornamental flowers, which general classes are not necessarily exclusive. Cereal plants, which produce an edible grain cereal, include, for example, corn, rice, wheat, barley, oat, rye, orchardgrass, guinea grass, sorghum and turfgrass. Leguminous plants include members of the pea family (Fabaceae) and produce a characteristic fruit known as a legume. Examples of leguminous plants include, for example, soybean, pea, chickpea, moth bean, broad bean, kidney bean, lima bean, lentil, cowpea, dry bean, and peanut, as well as alfalfa, birdsfoot trefoil, clover and sainfoin. Oilseed plants, which have seeds that are useful as a source of oil, include soybean, sunflower, rapeseed (canola) and cottonseed.

Angiosperms also include hardwood trees, which are perennial woody plants that generally have a single stem (trunk). Examples of such trees include alder, ash, aspen, basswood (linden), beech, birch, cherry, cottonwood, elm, eucalyptus, hickory, locust, maple, oak, persimmon, poplar, sycamore, walnut, sequoia, and willow. Trees are useful, for example, as a source of pulp, paper, structural material and fuel.

Angiosperms are fruit-bearing plants that produce a mature, ripened ovary, which generally contains seeds. A fruit can be suitable for human or animal consumption or for collection of seeds to propagate the species. For example, hops are a member of the mulberry family that are prized for their flavoring in malt liquor. Fruit-bearing angiosperms also include grape, orange, lemon, grapefruit, avocado, date, peach, cherry, olive, plum, coconut, apple and pear trees and blackberry, blueberry, raspberry, strawberry, pineapple, tomato, cucumber and eggplant plants. An ornamental flower is an angiosperm cultivated for its decorative flower. Examples of commercially important ornamental flowers include rose, orchid, lily, tulip and chrysanthemum, snapdragon, camellia, carnation and petunia plants. The skilled artisan will recognize that the methods of the invention can be practiced using these or other angiosperms, as desired, as well as gymnosperms, which do not produce seeds in a fruit.

A method of producing a transgenic plant can be performed by introducing a polynucleotide portion of plant stress-regulated gene into a plant cell genome, whereby the polynucleotide portion of the plant stress-regulated gene modulates a response of the plant cell to a stress condition, thereby producing a transgenic plant, which comprises plant cells that exhibit altered responsiveness to the stress condition. In one embodiment, the polynucleotide portion of the plant stress-regulated gene encodes a stress-regulated polypeptide or functional peptide portion thereof, wherein expression of the stress-regulated polypeptide or functional peptide portion thereof either increases the stress tolerance of the transgenic plant, or decreases the stress tolerance of the transgenic plant. The polynucleotide portion of the plant stress-regulated gene encoding the stress-regulated polypeptide or functional peptide portion thereof can be operatively linked to a heterologous promoter.

In another embodiment, the polynucleotide portion of the plant stress-regulated gene comprises a stress-regulated regulatory element. The stress-regulated regulatory element can integrate into the plant cell genome in a site-specific manner, whereupon it can be operatively linked to an endogenous nucleotide sequence, which can be expressed in response to a stress condition specific for the regulatory element; or can be a mutant regulatory element, which is not responsive to the stress condition, whereby upon integrating into the plant cell genome, the mutant regulatory element disrupts an endogenous stress-regulated regulatory element of a plant stress-regulated gene, thereby altering the responsiveness of the plant stress-regulated gene to the stress condition. Accordingly, the invention also provides genetically modified plants, including transgenic plants, produced by such a method, and a plant cell obtained from such genetically modified plant, wherein said plant cell exhibits altered responsiveness to the stress condition; a seed produced by a transgenic plant; and a cDNA library prepared from a transgenic plant.

Also provided is a method of modulating the responsiveness of a plant cell to a stress condition. Such a method can be performed, for example, by introducing a polynucleotide portion of a plant stress-regulated gene into the plant cell, thereby modulating the responsiveness of the plant cell to a stress condition. As disclosed herein, the responsiveness of the plant cell can be increased or decreased upon exposure to the stress condition, and the altered responsiveness can result in increased or decreased tolerance of the plant cell to a stress condition. The polynucleotide portion of the plant stress-regulated gene can, but need not, be integrated into the genome of the plant cell, thereby modulating the responsiveness of the plant cell to the stress condition. Accordingly, the invention also provide a genetically modified plant, including a transgenic plant, which contains an introduced polynucleotide portion of a plant stress-regulated gene, as well as plant cells, tissues, and the like, which exhibit modulated responsiveness to a stress condition.

The polynucleotide portion of the plant stress-regulated gene can encode a stress-regulated polypeptide or functional peptide portion thereof, which can be operatively linked to a heterologous promoter. As used herein, reference to a “functional peptide portion of a plant stress-regulated polypeptide” means a contiguous amino acid sequence of the polypeptide that has an activity of the full length polypeptide, or that has an antagonist activity with respect to the fall length polypeptide, or that presents an epitope unique to the polypeptide. Thus, by expressing a functional peptide portion of a plant stress-regulated polypeptide in a plant cell, the peptide can act as an agonist or an antagonist of the polypeptide, thereby modulating the responsiveness of the plant cell to a stress condition.

A polynucleotide portion of the plant stress-regulated nucleotide sequence also can contain a mutation, whereby upon integrating into the plant cell genome, the polynucleotide disrupts (knocks-out) an endogenous plant stress-regulated nucleotide sequence, thereby modulating the responsiveness of said plant cell to the stress condition. Depending on whether the knocked-out gene encodes an adaptive or a maladaptive stress-regulated polypeptide, the responsiveness of the plant will be modulated accordingly. Thus, a method of the invention provides a means of producing a transgenic plant having a knock-out phenotype of a plant stress-regulated nucleotide sequence.

Alternatively, the responsiveness of a plant or plant cell to a stress condition can be modulated by use of a suppressor construct containing dominant negative mutation for any of the stress-regulated sequences described herein. Expression of a suppressor construct containing a dominant mutant mutation generates a mutant transcript that, when coexpressed with the wild-type transcript inhibits the action of the wild-type transcript. Methods for the design and use of dominant negative constructs are well known (see, for example, in Herskowitz, Nature 329:219–222, 1987; Lagna and Hemmati-Brivanlou, Curr. Topics Devel. Biol. 36:75–98, 1998).

The polynucleotide portion of the plant stress-regulated gene also can comprise a stress-regulated regulatory element, which can be operatively linked to a heterologous nucleotide sequence, which, upon expression from the regulatory element in response to a stress condition, modulates the responsiveness of the plant cell to the stress condition. Such a heterologous nucleotide sequence can encode, for example, a stress-inducible transcription factor such as DREB1A, which, upon exposure to the stress condition, is expressed such that it can amplify the stress response (see Kasuga et al., supra, 1999). The heterologous nucleotide sequence also can encode a polynucleotide that is specific for a plant stress-regulated gene, for example, an antisense molecule, a ribozyme, and a triplexing agent, either of which, upon expression in the plant cell, reduces or inhibits expression of a stress-regulated polypeptide encoded by the gene, thereby modulating the responsiveness of the plant cell to a stress condition, for example, an abnormal level of cold, osmotic pressure, and salinity. As used herein, the term “abnormal,” when used in reference to a condition such as temperature, osmotic pressure, salinity, or any other condition that can be a stress condition, means that the condition varies sufficiently from a range generally considered optimum for growth of a plant that the condition results in an induction of a stress response in a plant. Methods of determining whether a stress response has been induced in a plant are disclosed herein or otherwise known in the art.

A plant stress-regulated regulatory element can be operatively linked to a heterologous polynucleotide sequence, such that the regulatory element can be introduced into a plant genome in a site-specific matter by homologous recombination. For example, a mutant plant stress-regulated regulatory element for a maladaptive stress-induced polypeptide can be transformed into a plant genome in a site specific manner by in vivo mutagenesis, using a hybrid RNA-DNA oligonucleotide (“chimeroplast” (TIBTECH 15:441–447, 1997; WO 95/15972; Kren, Hepatology 25:1462–1468, 1997; Cole-Strauss, Science 273:1386–1389, 1996, each of which is incorporated herein by reference). Part of the DNA component of the RNA-DNA oligonucleotide is homologous to a nucleotide sequence comprising the regulatory element of the maladaptive gene, but includes a mutation or contains a heterologous region which is surrounded by the homologous regions. By means of base pairing of the homologous regions of the RNA-DNA oligonucleotide and of the endogenous nucleic acid molecule, followed by a homologous recombination the mutation contained in the DNA component of the RNA-DNA oligonucleotide or the heterologous region can be transferred to the plant genome, resulting in a “mutant” gene that, for example, is not induced in response to a stress and, therefore, does not confer the maladaptive phenotype. Such a method similarly can be used to knock-out the activity of a stress-regulated gene, for example, in an undesirable plant. Such a method can provide the advantage that a desirable wild-type plant need not compete with the undesirable plant, for example, for light, nutrients, or the like.

A method of modulating the responsiveness of a plant cell to a stress condition also can be performed by introducing a mutation in the chromosomal copy of a plant stress-regulated gene, for example, in the stress-regulated regulatory element, by transforming a cell with a chimeric oligonucleotide composed of a contiguous stretch of RNA and DNA residues in a duplex conformation with double hairpin caps on the ends. An additional feature of the oligonucleotide is the presence of 2′-0-methylation at the RNA residues. The RNA/DNA sequence is designed to align with the sequence of a chromosomal copy of the target regulatory element and to contain the desired nucleotide change (see U.S. Pat. No. 5,501,967, which is incorporated herein by reference).

A plant stress-regulated regulatory element also can be operatively linked to a heterologous polynucleotide such that, upon expression from the regulatory element in the plant cell, confers a desirable phenotype on the plant cell. For example, the heterologous polynucleotide can encode an aptamer, which can bind to a stress-induced polypeptide. Aptamers are nucleic acid molecules that are selected based on their ability to bind to and inhibit the activity of a protein or metabolite. Aptamers can be obtained by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) method (see U.S. Pat. No. 5,270,163), wherein a candidate mixture of single stranded nucleic acids having regions of randomized sequence is contacted with a target, and those nucleic acids having a specific affinity to the target are partitioned from the remainder of the candidate mixture, and amplified to yield a ligand enriched mixture. After several iterations a nucleic acid molecule (aptamer) having optimal affinity for the target is obtained. For example, such a nucleic acid molecule can be operatively linked to a plant stress-regulated regulatory element and introduced into a plant. Where the aptamer is selected for binding to a polypeptide that normally is expressed from the regulatory element and is involved in an adaptive response of the plant to a stress, the recombinant molecule comprising the aptamer can be useful for inhibiting the activity of the stress-regulated polypeptide, thereby decreasing the tolerance of the plant to the stress condition.

The invention provides a genetically modified plant, which can be a transgenic plant, that is tolerant or resistant to a stress condition. As used herein, the term “tolerant” or “resistant,” when used in reference to a stress condition of a plant, means that the particular plant, when exposed to a stress condition, shows less of an effect, or no effect, in response to the condition as compared to a corresponding reference plant (naturally occurring wild-type plant or a plant not containing a construct of the present invention). As a consequence, a plant encompassed within the present invention grows better under more widely varying conditions, has higher yields and/or produces more seeds. Thus, a transgenic plant produced according to a method of the invention can demonstrate protection (as compared to a corresponding reference plant) from a delay to complete inhibition of alteration in cellular metabolism, or reduced cell growth or cell death caused by the stress. Preferably, the transgenic plant is capable of substantially normal growth under environmental conditions where the corresponding reference plant shows reduced growth, metabolism or viability, or increased male or female sterility.

The determination that a plant modified according to a method of the invention has increased resistance to a stress-inducing condition can be made by comparing the treated plant with a control (reference) plant using well known methods. For example, a plant having increased tolerance to saline stress can be identified by growing the plant on a medium such as soil, which contains a higher content of salt in the order of at least about 10% compared to a medium the corresponding reference plant is capable of growing on. Advantageously, a plant treated according to a method of the invention can grow on a medium or soil containing at least about 50%, or more than about 75%, particularly at least about more than 100%, and preferably more than about 200% salt than the medium or soil on which a corresponding reference plant can grow. In particular, such a treated plant can grow on medium or soil containing at least 40 mM, generally at least 100 mM, particularly at least 200 mM, and preferably at least 300 mM salt, including, for example, a water soluble inorganic salt such as sodium sulfate, magnesium sulfate, calcium sulfate, sodium chloride, magnesium chloride, calcium chloride, potassium chloride, or the like; salts of agricultural fertilizers, and salts associated with alkaline or acid soil conditions; particularly NaCl.

In another embodiment, the invention provides a plant that is less tolerant or less resistant to a stress condition as compared to a corresponding reference plant. As used herein, the term “less tolerant” or “less resistant,” when used in reference to a stress condition of a plant, means that the particular plant, when exposed to a stress condition, shows an alteration in response to the condition as compared to a corresponding reference plant. As a consequence, such a plant, which generally is an undesirable plant species, is less likely to grow when exposed to a stress condition than an untreated plant.

The present invention also relates to a method of expressing a heterologous nucleotide sequence in a plant cell. Such a method can be performed, for example, by introducing into the plant cell a plant stress-regulated regulatory element operatively linked to the heterologous nucleotide sequence, whereby, upon exposure of the plant cell to stress condition, the heterologous nucleotide sequence is expressed in the plant cell. The heterologous nucleotide sequence can encode a selectable marker, or preferably, a polypeptide that confers a desirable trait upon the plant cell, for example, a polypeptide that improves the nutritional value, digestibility or ornamental value of the plant cell, or a plant comprising the plant cell. Accordingly, the invention provides a transgenic plant that, in response to a stress condition, can produce a heterologous polypeptide from a plant stress-regulated regulatory element. Such transgenic plants can provide the advantage that, when grown in a cold environment for example, expression of the heterologous polypeptide from a plant cold-regulated regulatory element can result in increased nutritional value of the plant.

The present invention further relates to a method of modulating the activity of a biological pathway in a plant cell, wherein the pathway involves a stress-regulated polypeptide. As used herein, reference to a pathway that “involves” a stress-regulated polypeptide means that the polypeptide is required for normal function of the pathway. For example, plant stress-regulated polypeptides as disclosed herein include those acting as kinases or as transcription factors, which are well known to be involved in signal transduction pathways. As such, a method of the invention provides a means to modulate biological pathways involving plant stress-regulated polypeptides, for example, by altering the expression of the polypeptides in response to a stress condition. Thus, a method of the invention can be performed, for example, by introducing a polynucleotide portion of a plant stress-regulated gene into the plant cell, thereby modulating the activity of the biological pathway.

A method of the invention can be performed with respect to a pathway involving any of the stress-regulated polypeptides as encoded by a polynucleotide of SEQ ID NOS:1–2703, including for example, a stress-regulated transcription factor, an enzyme, including a kinase, a channel protein (see, for example, Tables 29–31; see, also, Table 1). Pathways in which the disclosed stress-regulated stress factors are involved can be identified, for example, by searching the Munich Information Center for Protein Sequences (MIPS) Arabidopsis thaliana database (MATDB), which is at http://www.mips.biochem.mpg.de/proj/thal/.

The present invention also relates to a method of identifying a polynucleotide that modulates a stress response in a plant cell. Such a method can be performed, for example, by contacting an array of probes representative of a plant cell genome and nucleic acid molecules expressed in plant cell exposed to the stress; detecting a nucleic acid molecule that is expressed at a level different from a level of expression in the absence of the stress; introducing the nucleic acid molecule that is expressed differently into a plant cell; and detecting a modulated response of the plant cell containing the introduced nucleic acid molecule to a stress, thereby identifying a polynucleotide that modulates a stress response in a plant cell. The contacting is under conditions that allow for selective hybridization of a nucleic acid molecule with probe having sufficient complementarity, for example, under stringent hybridization conditions.

As used herein, the term “array of probes representative of a plant cell genome” means an organized group of oligonucleotide probes that are linked to a solid support, for example, a microchip or a glass slide, wherein the probes can hybridize specifically and selectively to nucleic acid molecules expressed in a plant cell. Such an array is exemplified herein by a GeneChip® Arabidopsis Genome Array (Affymetrix; see Example 1). In general, an array of probes that is “representative” of a plant genome will identify at least about 30% or the expressed nucleic acid molecules in a plant cell, generally at least about 50% or 70%, particularly at least about 80% or 90%, and preferably will identify all of the expressed nucleic acid molecules. It should be recognized that the greater the representation, the more likely all nucleotide sequences of cluster of stress-regulated genes will be identified.

A method of the invention is exemplified in Example 1, wherein clusters of Arabidopsis genes induced to cold, to increased salinity, to increased osmotic pressure, and to a combination of the above three stress conditions were identified. Based on the present disclosure, the artisan readily can obtain nucleic acid samples for Arabidopsis plants exposed to other stress conditions, or combinations of stress conditions, and identify clusters of genes induced in response to the stress conditions. Similarly, the method is readily adaptable to identifying clusters of stress-regulated genes expressed in other plant species, particularly commercially valuable plant species, where a substantial amount of information is known regarding the genome.

The clusters of genes identified herein include those clusters of genes that are induced or repressed in response to a combination of stress conditions, but not to any of the stress conditions alone; and clusters of genes that are induced or repressed in response to a selected stress condition, but not to other stress conditions tested. Furthermore, clusters of genes that respond to a stress condition in a temporally regulated manner are also included, such as gene clusters that are induced early (for example, within about 3 hours), late (for example, after about 8 to 24 hours), or continuously in a stress response. In addition, the genes within a cluster are represented by a variety of cellular proteins, including transcription factors, enzymes such as kinases, channel proteins, and the like (see Tables 1 and 29–31). Thus, the present invention further characterizes nucleotide sequences that previously were known to encode cellular peptides by classifying them within clusters of stress-regulated genes.

The present invention additionally relates to a method of identifying a stress condition to which a plant cell was exposed. Such a method can be performed, for example, by contacting nucleic acid molecules expressed in the plant cell and an array of probes representative of the plant cell genome; and detecting a profile of expressed nucleic acid molecules characteristic of a stress response, thereby identifying the stress condition to which the plant cell was exposed. The contacting generally is under conditions that allow for selective hybridization of a nucleic acid molecule with probe having sufficient complementarity, for example, under stringent hybridization conditions. The profile can be characteristic of exposure to a single stress condition, for example, an abnormal level of cold, osmotic pressure, or salinity (Tables 3–14), or can be characteristic of exposure to more than one stress condition (Tables 15–26, for example, cold, increased osmotic pressure and increased salinity (see Tables 24–26).

The method can be practiced using at least one nucleic acid probe and can identify one or combination of stress conditions by detecting altered expression of one or a plurality of polynucleotides representative of plant stress-regulated genes. As used herein, the term “at least one” includes one, two, three or more, for example, five, ten, twenty, fifty or more polynucleotides, nucleic acid probes, and the like. The term “plurality” is used herein to mean two or more, for example, three, four, five or more, including ten, twenty, fifty or more polynucleotides, nucleic acid probes, and the like.

In a method of the invention, nucleic acid samples from the plant cells to be collected can be contacted with an array, then the profile can be compared with known expression profiles prepared from nucleic acid samples of plants exposed to a known stress condition or combination of stress conditions. By creating a panel of such profiles, representative of various stress conditions, an unknown stress condition to which a plant was exposed can be identified simply by comparing the unknown profile with the known profiles and determining which known profile that matches the unknown profile. Preferably, the comparison is automated. Such a method can be useful, for example, to identify a cause of damage to a crop, where the condition causing the stress is not known or gradually increases over time. For example, accumulation in soils over time of salts from irrigation water can result in gradually decreasing crop yields. Because the accumulation is gradual, the cause of the decreased yield may not be readily apparent. Using the present methods, it is possible to evaluate the stress to which the plants are exposed, thus revealing the cause of the decreased yields.

The present invention, therefore includes a computer readable medium containing executable instructions form receiving expression data for sequences substantially similar to any of those disclosed herein and comparing expression data from a test plant to a reference plant that has been exposed to an abiotic stress. Also provided is a computer-readable medium containing sequence data for sequences substantially similar to any of the sequences described herein, or the complements thereof, and a module for comparing such sequences to other nucleic acid sequences.

Also provided are plants and plant cells comprising plant stress-regulatory elements of the present invention operably linked to a nucleotide sequence encoding a detectable signal. Such plants can be used as diagnostic or “sentinel” plants to provide early warning that nearby plants are being stressed so that appropriate actions can be taken. In one embodiment, the signal is one that alters the appearance of the plant. For example, an osmotic stress regulatory element of the present invention can be operably linked to a nucleotide sequence encoding a fluorescent protein such as green fluorescent protein. When subjected to osmotic stress, the expression of the green fluorescent protein in the sentinel plant provides a visible signal so that appropriate actions can be taken to remove or alleviate the stress. The use of fluorescent proteins in plants is well known (see, for example, in Leffel et al., BioTechniques 23:912, 1997).

The invention further relates to a method of identifying an agent that modulates the activity of a stress-regulated regulatory element of a plant. As used herein, the term “modulate the activity,” when used in reference to a plant stress-regulated regulatory element, means that expression of a polynucleotide from the regulatory element is increased or decreased. In particular, expression can be increased or decreased with respect to the basal activity of the promoter, i.e., the level of expression, if any, in the absence of a stress condition that normally induces expression from the regulatory element; or can be increased or decreased with respect to the level of expression in the presence of the inducing stress condition. As such, an agent can act as a mimic of a stress condition, or can act to modulate the response to a stress condition.

Such a method can be performed, for example, by contacting the regulatory element with an agent suspected of having the ability to modulate the activity of the regulatory element, and detecting a change in the activity of the regulatory element. In one embodiment, the regulatory element can be operatively linked to a heterologous polynucleotide encoding a reporter molecule, and an agent that modulates the activity of the stress-regulated regulatory element can be identified by detecting a change in expression of the reporter molecule due to contacting the regulatory element with the agent. Such a method can be performed in vitro in a plant cell-free system, or in a plant cell in culture or in a plant in situ.

A method of the invention also can be performed by contacting the agent is contacted with a genetically modified cell or a transgenic plant containing an introduced plant stress-regulated regulatory element, and an agent that modulates the activity of the regulatory element is identified by detecting a phenotypic change in the modified cell or transgenic plant.

A method of the invention can be performed in the presence or absence of the stress condition to which the particularly regulatory element is responsive. As such, the method can identify an agent that modulates the activity of plant stress-regulated promoter in response to the stress, for example, an agent that can enhance the stress response or can reduce the stress response. In particular, a method of the invention can identify an agent that selectively activates the stress-regulated regulatory elements of a cluster of plant stress-regulated genes, but does not affect the activity of other stress-regulated regulatory genes. As such, the method provides a means to identify an agent that acts as a stress mimic. Such agents can be particularly useful to prepare a plant to an expected stress condition. For example, a agent that acts as a cold mimic can be applied to a field of plants prior to the arrival of an expected cold front. Thus, the cold stress response can be induced prior to the actual cold weather, thereby providing the plants with the protection of the stress response, without the plants suffering from any initial damage due to the cold. Similarly, an osmotic pressure mimic can be applied to a crop of plants prior a field being flooded by a rising river.

In one embodiment, the present invention provides a method for marker-assisted selection. Marker-assisted selection involves the selection of plants having desirable phenotypes based on the presence of particular nucleotide sequences (“markers”). The use of markers allows plants to be selected early in development, often before the phenotype would normally be manifest. Because it allows for early selection, marker-assisted selection decreases the amount of time need for selection and thus allows more rapid genetic progress.

Briefly, marker-assisted selection involves obtaining nucleic acid from a plant to be selected. The nucleic acid obtained is then probed with probes that selectively hybridize under stringent, preferably highly stringent, conditions to a nucleotide sequence or sequences associated with the desired phenotype. In one embodiment, the probes hybridize to any of the stress-responsive genes or regulatory regions disclosed herein, for example, any one of SEQ ID NOS:1–2703. The presence of any hybridization products formed is detected and plants are then selected on the presence or absence of the hybridization products.

The following examples are intended to illustrate but not limit the invention.

EXAMPLE 1
Profiling of Plant Stress-Regulated Genes

This example demonstrates that clusters of stress-regulated genes can be identified in plant cells exposed to various stress conditions, either alone or in combination.

A GeneChip® Arabidopsis Genome Array (Affymetrix, Santa Clara, Calif.) was used to identify clusters of genes that were coordinately induced in response to various stress conditions. The GeneChip® Arabidopsis Genome Array contains probes synthesized in situ and is designed to measure temporal and spatial gene expression of approximately 8700 genes in greater than 100 EST clusters. The sequences used to develop the array were obtained from GenBank (http://www.ncbi.nlm.nih.gov/) in collaboration with Torrey Mesa Research Institute (San Diego, Calif.), formerly known as Novartis Agriculture Discovery Institute. Eighty percent of the nucleotide sequences represented on the array are predicted coding sequences from genomic BAC entries; twenty percent are high quality cDNA sequences. The array also contains over 100 EST clusters that share homology with the predicted coding sequences from BAC clones (see, for example, world wide web at address (url) “affymetrix.com/products/Arabidopsis_content.html”.

The Affymetrix GeneChip® array was used to define nucleotide sequences/pathways affected by various abiotic stresses and to define which are uniquely regulated by one stress and those that respond to multiple stress, and to identify candidate nucleotide sequences for screening for insertional mutants. Of the approximately 8,700 nucleotide sequences represented on the Affymetrix GeneChip® array, 2862 nucleotide sequences showed at least a 2-fold change in expression in at least one sample, relative to no-treatment controls. Of those 2,862 nucleotide sequences 1,335 were regulated only by cold stress, 166 were regulated only mannitol stress and 209 were regulated only by saline stress. Furthermore, of the 2,862 nucleotide sequences 123 nucleotide sequences were regulated by salt and mannitol stress, 293 were regulated by mannitol and cold stress, 274 were regulated by cold and saline stress and 462 were regulated by cold, mannitol and salt. Of the 2,862 nucleotide sequences, 771 passed the higher stringency of showing at least a 2-fold change in expression in at least 2 samples, relative to control. And, 508 of the 771 nucleotide sequences were found in an in-house collection of insertion mutants.

The following describes in more detail how the experiments were done. Transcriptional profiling was performed by hybridizing fluorescence labeled cRNA with the oligonucleotides probes on the chip, washing, and scanning. Each gene is represented on the chip by about sixteen oligonucleotides (25-mers). Expression level is related to fluorescence intensity. Starting material contained 1 to 10 μg total RNA; detection specificity was about 1:10⁶; approximately a 2-fold change was detectable, with less than 2% false positive; the dynamic range was approximately 500×. Nucleotide sequences having up to 70% to 80% identity could be discriminated using this system.

Seven day old axenic Arabidopsis seedlings were transferred to Magenta boxes with rafts floating on MS medium. Three weeks later (28 day old seedlings), stresses were applied as follows: Control−no treatment; Cold−Magenta box placed in ice; Mannitol−medium+200 mM mannitol; Salt−medium+100 mM NaCl. Tissue samples were collected at 3 hours and 27 hours into the stress, roots and aerial portions were harvested, RNA was purified, and the samples were analyzed using the GeneChip® Arabidopsis Genome Array (Affymetrix, Santa Clara, Calif.) following the manufacturer's protocol.

Raw fluorescence values as generated by Affymetrix software were processed as follows: the values were brought into Microsoft Excel and values of 25 or less were set to 25 (an empirically determined baseline, Zhu and Wang, Plant Physiol. 124:1472–1476; 2000). The values from the stressed samples were then converted to fold change relative to control by dividing the values from the stressed samples by the values from the no-treatment control samples. Expression patterns that were altered at least 2-fold with respect to the control were selected. This method gave very robust results and resulted in a larger number of nucleotide sequences called as stress-regulated than previous methods had permitted.

Based on the profiles obtained following hybridization of nucleic acid molecules obtained from plant cells exposed to various stress conditions to the probes in the microarray, clusters of nucleotide sequences that were altered in response to the stress conditions were identified (see Tables 3–6, cold responsive; Tables 7–10, salt (saline) responsive; Tables 11 to 14, mannitol (osmotic) responsive; Tables 15–17, cold and mannitol responsive; Tables 18–20, 6 salt and cold responsive; Tables 21–23, salt and mannitol responsive; Tables 24–26, cold, salt and mannitol responsive. Examples of plant gene sequences that varied in expression at least two-fold in response to a combination of cold, saline and osmotic stress in root cells and leaf cells are shown in Tables 27 and 28, respectively. In addition, examples of plant gene sequences that encode transcription factors (Table 29), phosphatases (Table 30), and kinases (Table 31) and that varied at least two-fold in response to a combination of cold, saline and osmotic stress are provided.

Affymetrix ID numbers and corresponding SEQ ID NOS: for the respective Arabidopsis nucleotide sequences are provided Tables 3–26, and can be used to determine SEQ ID NOS: for the sequences shown by Affymetrix ID number in Tables 27–31. The Affymetrix ID number refers to a particular nucleotide sequence on the GeneChip® Arabidopsis Genome Array. In some cases, a particular plant stress-regulated gene sequence hybridized to more than one nucleotide sequence on the GeneChip® Arabidopsis Genome Array (see, for example, Table 3, where SEQ ID NO:36 is shown to have hybridized to the 12187_AT and 15920_I_AT nucleotide sequences on the GeneChip®). In addition, it should be recognized that the disclosed sequences are not limited to coding sequences but, in some cases, include 5′ untranslated sequences (see Table 2) or a longest coding region. As such, while the sequences set forth as SEQ ID NOS:1–2073 generally start with an ATG codon, in most cases each comprises a longer nucleotide sequence, including a regulatory region (see Table 2).

The results disclosed herein demonstrate that several polynucleotides, some of which were known to function as transcription factors, enzymes, and structural proteins, also are involved in the response of a plant cell to stress. The identification of the clusters of stress-regulated genes as disclosed herein provides a means to identify stress-regulated regulatory elements present in Arabidopsis thaliana nucleotide sequences, including consensus regulatory elements. It should be recognized, however that the regulatory elements of the plant genes comprising a sequence as set forth in SEQ ID NOS:156, 229, 233, 558, 573, 606, 625, 635, 787, and 813, which previously have been described as cold regulated genes, are not encompassed within the stress-regulated gene regulatory element of the invention, and the regulatory elements of the plant genes comprising the nucleotide sequences set forthas SEQ ID NOS:1263, 1386, 1391, 1405, 1445, 1484, 1589, 1609, 1634, 1726, 1866, 1918, and 1928, which previously have been identified as genes that are responsive to a single stress condition such as cold or saline stress, are not encompassed within the plant stress-regulated gene regulatory elements of the invention to the extent that they confer stress-regulated expression only with respect to the known single stress. Furthermore, the identification of the Arabidopsis stress-regulated genes provides a means to identify the corresponding homologs and orthologs in other plants, including commercially valuable food crops such as wheat, rice, soy, and barley, and ornamental plants. BLASTN and BLASTP searches to identify such sequences revealed the polynucleotide sequences set forth in Table 32, which is on the CD-R compact disc submitted herewith.

Although the invention has been described with reference to the above example, it will be understood that modifications and variations are encompassed within the spirit and scope of the invention. Accordingly, the invention is limited only by the claims, which follow Tables 1 to 31.

TABLE 1

SEQUENCE DESCRIPTIONS

Seq

Seq

ID
Description
ID
Description

1
unknown protein
1352
bZIP transcription factor-like

protein

2
unknown protein
1353
Medicago nodulin N21-like protein

3
unknown protein
1354
putative endo-1,4-beta glucanase

4
putative auxin-induced
1355
1-aminocyclopropane-1-

protein

carboxylate oxidase

5
unknown protein
1356
putative anion exchange protein

6
hypothetical protein
1357
SRG1-like protein

7
putative protein
1358
putative protein

8
unknown protein
1359
putative phi-1-like phosphate-

induced protein

9
unknown protein
1360
putative protein

10
unknown protein
1361
putative embryo-abundant protein

11
putative protein
1362
putative hydrolase

12
Thioredoxin-like protein
1363
unknown protein

13
putative RNA helicase
1364
unknown protein

14
putative protein
1365
hexose transporter-like protein

15
putative protein
1366
unknown protein

16
RING zinc finger protein,
1367
unknown protein

putative

17
putative cyclin
1368
peptide transport-like protein

18
putative protein
1369
unknown protein

19
putative protein
1370
putative peptide transporter

20
unknown protein
1371
disease resistance protein, putative

21
putative protein
1372
cysteine protease component of

protease-inhibitor complex

22
putative protein
1373
putative cytochrome P450

23
hypothetical protein
1374
putative protein

24
unknown protein
1375
hypothetical protein

25
hypothetical protein
1376
unknown protein

26
unknown protein
1377
putative

phosphoribosylaminoimidazolecar

boxamide formyltransferase

27
unknown protein
1378
putative protein

28
unknown protein
1379
HSP like protein

29
unknown protein
1380
unknown protein

30
putative protein
1381
unknown protein

31
putative protein
1382
putative cytochrome P450

32
putative protein
1383
similar to pectinesterase

33
unknown protein
1384
putative glucosyltransferase

34
putative ribonuclease III
1385
thaumatin-like protein

35
unknown protein
1386
drought-inducible cysteine

proteinase RD19A precursor

36
unknown protein
1387
vegetative storage protein Vsp2

37
unknown protein
1388
unknown protein

38
unknown protein
1389
unknown protein

39
unknown protein
1390
anthranilate N-

benzoyltransferase-like

protein

40
putative histidine kinase
1391
delta-1-pyrroline 5-

carboxylase synthetase

(P5C1)

41
scarecrow-like 7 (SCL7)
1392
glutathione S-conjugate

transporting ATPase

(AtMRP1)

42
putative protein
1393
hypothetical protein

43
No function assigned by TIGR
1394
hypothetical protein

44
unknown protein
1395
unknown protein

45
unknown protein
1396
putative protein

46
succinyl-CoA-ligase alpha subunit
1397
putative protein

47
putative protein
1398
No function assigned by

TIGR

48
CLV1 receptor kinase like protein
1399
unknown protein

49
putative receptor-like protein
1400
putative protein kinase

kinase

50
putative squalene synthase
1401
unknown protein

51
putative receptor protein kinase
1402
hypothetical protein

52
somatic embryogenesis receptor-
1403
unknown protein

like kinase, putative

53
putative protein
1404
putative calcium-binding

EF-hand protein

54
putative beta-glucosidase
1405
cinnamyl-alcohol

dehydrogenase ELI3-1

55
multi-drug resistance protein
1406
putative protein

56
receptor protein kinase (TMK1),
1407
unknown protein

putative

57
putative receptor-like protein
1408
senescence-associated

kinase

protein sen1

58
putative pectate lyase
1409
hypothetical protein

59
putative protein kinase
1410
putative cytochrome P450

60
putative peroxidase
1411
proline oxidase,

mitochondrial precursor

(osmotic stress-induced

proline dehydrogenase)

61
cytochrome P450-like protein
1412
putative response regulator

3

62
putative beta-amylase
1413
hypothetical protein

63
monosaccharide transporter STP3
1414
glutamine-dependent

asparagine synthetase

64

Lycopersicon esculentum

1415
lysine-ketoglutarate

proteinase TMP, Pir2:T07617

reductase/saccharopine

65
putative receptor-like protein
1416
En/Spm-like transposon

kinase

protein

66
G-box-binding factor 1
1417
G-box binding bZIP transcription

factor

67
amino acid carrier, putative
1418
putative protein

68
myb-related protein
1419
putative protein

69
No function assigned by TIGR
1420
putative protein

70
SNF1 like protein kinase
1421
ATFP4-like

71
Cu/Zn superoxide dismutase-like
1422
unknown protein

protein

72
putative protein kinase
1423
unknown protein

73
small nuclear ribonucleoprotein
1424
putative protein

U1A

74
ras-like GIF-binding
1425
invertase inhibitor homolog

protein

(emb|CAA73335.1)

75
oleoyl-[acyl-carrier-protein]
1426
unknown protein

hydrolase-like protein

76
putative heat shock
1427
unknown protein

transcription factor

77
putative protein
1428
putative cytochrome b5

78
membrane-bound small
1429
putative protein

GTP-binding-like protein

79
putative protein (fragment)
1430
putative protein

80
indole-3-acetate beta-
1431
putative protein

glucosyltransferase like

protein

81
HD-zip transcription factor
1432
No function assigned by TIGR

(athb-8)

82
putative cAMP-dependent
1433
putative copper/zinc superoxide

protein kinase

dismutase

83
glucuronosyl transferase-
1434
protein phosphatase ABI1

like protein

84
putative leucine-rich repeat
1435
glutamate dehydrogenase 2

disease resistance protein

85
98b like protein
1436
No function assigned by TIGR

86
putative receptor-like
1437
low-temperature-induced protein

protein kinase

78 (sp|Q06738)

87
IAA-Ala hydrolase (IAR3)
1438
putative myo-inositol 1-phosphate

synthase

88
putative AP2 domain
1439
phosphate transporter

transcription factor

(gb|AAB17265.1)

89
putative expansin
1440
4-hydroxyphenylpyruvate

dioxygenase (HPD)

90
putative Ap2 domain
1441
histone H1

protein

91
expansin (At-EXP1)
1442
hypothetical protein

92
cytochrome P450-like
1443
No function assigned by TIGR

protein

93
putative ATP-dependent
1444
neoxanthin cleavage enzyme-like

RNA helicase A

protein

94
unknown protein
1445
dehydration-induced protein RD22

95
predicted protein
1446
zinc finger protein ZAT7

96
putative glucosyltransferase
1447
unknown protein

97
unknown protein
1448
unknown protein

98
putative xyloglucan-
1449
unknown protein

specific glucanase

99
cysteine synthase
1450
unknown protein

100
clathrin assembly protein
1451
putative protein

AP19 homolog

101
dynein light chain like protein
1452
putative protein

102
chaperonin CPN10
1453
RNA helicase, putative

103
putative bHLH transcription factor
1454
putative glycine-rich

protein

104
putative glyoxysomal malate
1455
hypothetical protein

dehydrogenase precursor

105
ATP-dependent RNA helicase,
1456
putative protein

putative

106
chlorophyll synthetase
1457
peroxidase

107
similar to epoxide hydrolases
1458
peroxidase ATP3a

(emb|CAA67340.1)

108
putative protein
1459
metallothionein-like protein

109
unknown protein
1460
endomembrane-associated

protein

110
hypothetical protein
1461
ferritin 1 precursor

111
putative membrane transporter
1462
dehydrin RAB18-like

protein (sp|P30185)

112
putative tyrosyl-tRNA synthetase
1463
HSR201 like protein

113
ARGININE/SERINE-RICH
1464
light regulated protein,

SPLICING FACTOR RSP31

putative

114
putative oxidoreductase
1465
Dr4 (protease inhibitor)

115
unknown protein
1466
mitogen activated protein

kinase kinase (nMAPKK)

116
linker histone protein, putative
1467
glutathione S-transferase

117
hypothetical protein
1468
transcriptional activator

CBF1/CRT/CRE binding

factor 1

118
putative protein
1469
homeobox-leucine zipper

protein ATHB-12

119
putative mitochondrial carrier
1470
amino acid permease I

protein

120
putative transcription factor
1471
MAP kinase (ATMPK7)

121
MYB-related protein
1472
potassium channel protein

AKT3

122
myb-related transcription factor,
1473
cytochrome P450

putative

monooxygenase

(CYP91A2)

123
unknown protein
1474
putative transport protein

124
unknown protein
1475
putative protein

125
putative glycine-rich protein
1476
hypothetical protein

126
No function assigned by TIGR
1477
putative protein

127
unknown protein
1478
hypothetical protein

128
unknown protein
1479
receptor protein kinase-like

protein

129
unknown protein
1480
serine/threonine protein

kinase-like protein

130
unknown protein
1481
putative auxin-regulated

protein

131
putative membrane channel protein
1482
amino acid transport protein

AAP2

132
putative protein
1483
unknown protein

133
unknown protein
1484
cold and ABA inducible protein

kin1

134
gamma glutamyl hydrolase,
1485
gamma-VPE (vacuolar processing

putative

enzyme)

135
40S ribosomal protein S5
1486
putative protein 1 photosystem II

oxygen-evolving complex

136
DnaJ-like protein
1487
myrosinase-associated protein,

putative

137
40S ribosomal protein S26
1488
transcription factor ATMYB4

138
putative WRKY-type DNA binding
1489
H-protein promoter binding factor-

protein

2a

139
putative protein
1490
ammonium transporter, puitative

140
hypothetical protein
1491
putative zeta-carotene desaturase

precursor

141
putative ubiquitin-
1492
high-affinity nitrate transporter

conjugating enzyme

NRT2

142
peptidylprolyl isomerase
1493
light induced protein like

ROC1

143
glyceraldehyde-3-
1494
putative AT-hook DNA-binding

phosphate dehydrogenase C

protein

subunit (GapC)

144
No function assigned by
1495
putative glycogenin

TIGR

145
putative protein
1496
putative light repressible receptor

protein kinase

146
putative thioredoxin
1497
serine/threonine kinase-like

protein

147
thioredoxin h, putative
1498
putative peroxidase

148
thioredoxin-like
1499
cytochrome P450 monooxygenase

(CYP83A1)

149
allene oxide synthase
1500
MYB-related transcription factor

(emb|CAA73184.1)

(CCA1)

150
anthranilate synthase
1501
Terminal flower1 (TFL1)

component I-1 precursor

(sp|P32068)

151
CELL DIVISION
1502
sulfate transporter ATST1

CONTROL PROTEIN 2

HOMOLOG A

152
protein kinase cdc2
1503
RING-H2 finger protein RHA3b

homolog B

153
ethylene responsive
1504
lipoxygenase, putative

element binding factor 1

(frameshift !)

154
ethylene responsive
1505
serine O-acetyltransferase (EC

element binding factor 2

2.3.1.30) Sat-52 (pir||S71207)

(ATERF2) (sp|O80338)

155
ethylene responsive
1506
ferulate-5-hydroxylase (FAH1)

element binding factor 5

(ATERF5) (sp|O80341)

156
glucose-6-phosphate
1507
En/Spm-like transposon protein,

dehydrogenase

putative

157
photomorphogenesis
1508
calmodulin-binding-like protein

represser (COP1)

158
unknown protein
1509
hypothetical protein

159
DNA (cytosine-5)- methyltransferase (DNA
1510
somatic embryogenesis receptor-

methyltransferase) (DNA

like kinase-like protein

metase) (sp|P34881)

160
PROLIFERA
1511
putative giberellin beta-

hydroxylase

161
putative photomorphogenesis
1512
putative pectinesterase

represser protein

162
SNF1-like protein kinase (AKin11)
1513
putative protein

163
thioredoxin h
1514
unknown protein

164
thioredoxin
1515
ribosomal protein

165
Ca2+-dependent lipid-binding
1516
low-temperature-induced

protein, putative

65 kD protein (sp|Q04980)

166
putative auxin-induced protein
1517
putative glucosyltransferase

167
putative bZIP transcription factor
1518
peroxidase

(emb|CAA67551.1)

168
hypothetical protein
1519
ankyrin-like protein

169
putative AVR9 elicitor response
1520
ribosomal protein S11-like

protein

170
putative serine/threonine protein
1521
hypothetical protein

kinase

171
bZIP transcription factor ATB2
1522
glycoprotein (EP1), putative

172
putative spliceosome associated
1523
calnexin-like protein

protein

173
3-hydroxyisobutyryl-coenzyme A
1524
SRG1-like protein

hydrolase-like protein

174
putative protein
1525
ethylene response factor 1

(ERF1)

175
putative Mutator-like transposase
1526
transcriptional activator

CBF1-like protein

176
putative protein
1527
xyloglucan endo-1,4-beta-

D-glucanase (XTR-6)

177
unknown protein
1528
putative cinnamyl alcohol

dehydrogenase

178
putative protein
1529
gibberellin 3 beta-

hydroxylase, putative

179
putative protein
1530
auxin response transcription

factor 3 (ETTIN/ARF3)

180
putative galactinol synthase
1531
No function assigned by

TIGR

181
putative transcriptional regulator
1532
putative protein

182
nuclear matrix constituent protein 1
1533
similar to avrRpt2-induced

(NMCP1)-like

protein 1

183
putative DNA-binding protein
1534
unknown protein

RAV2

184
No function assigned by TIGR
1535
hypothetical protein

185
basic blue protein, 5′ partial
1536
putative protein kinase

186
unknown protein
1537
respiratory burst oxidase-

like protein

187
putative calcium-binding protein,
1538
glucose-6-

calreticulin

phosphate/phosphate-

translocator precursor,

putative

188
putative pyrophosphate-fructose-6-
1539
class 1 non-symbiotic

phosphate 1-phosphotransferase

hemoglobin (AHB1)

189
ribosomal protein L11, cytosolic
1540
endochitinase isolog

190
putative dTDP-glucose 4-6-
1541
putative cytochrome P450

dehydratase

191
40S ribosomal protein S20-like
1542
60S acidic ribosomal protein P0

protein

192
60S ribosomal protein L24
1543
putative protein

193
coatomer-like protein,
1544
auxin-induced protein, putative

epsilon subunit

194
glycoprotein (EP1), putative
1545
unknown protein

195
putative SPL1-related
1546
hypothetical protein

protein

196
unknown protein
1547
protein phosphatase 2C ABI2

(PP2C) (sp|O04719)

197
putative transport protein
1548
peroxidase, prxr2

SEC61 beta-subunit

198
unknown protein
1549
putative peroxidase ATP12a

199
putative cytochrome P450
1550
putative beta-amylase

200
UTP-glucose
1551
putative acetone-cyanohydrin lyase

glucosyltransferase-like

protein

201
60S ribosomal protein L23
1552
fatty acid elongase 3-ketoacyl-CoA

synthase 1

202
40S ribosomal protein S17
1553
putative citrate synthase

203
40S ribosomal protein S26
1554
pEARLI 1-like protein

204
protein translation factor
1555
putative MYB family transcription

Sui1 homolog, putative

factor

205
unknown protein
1556
putative transcription factor

MYB28

206
gamma glutamyl hydrolase,
1557
RNA helicase-like protein

putative

207
dTDP-glucose 4,6-
1558
snoRNA

dehydratase, putative

208
extensin-like protein
1559
putative protein kinase

209
unknown protein
1560
growth regulator like protein

210
protein phosphatase 2C-
1561
putative potassium transporter

like protein

211
ubiquitin-like protein
1562
putative protein

212
protein phosphatase 2C-like
1563
60S ribosomal protein L14

protein

213
unknown protein
1564
unknown protein

214
putative RING zinc finger
1565
putative RING-H2 zinc finger

ankyrin protein

protein

215
unknown protein
1566
putative pollen surface protein

216
putative rubisco subunit
1567
unknown protein

binding-protein alpha

subunit

217
putative acetone-
1568
unknown protein

cyanohydrin lyase

218
putative isoamylase
1569
unknown protein

219
putative protein
1570
putative Ca2+-ATPase

220
HSP associated protein like
1571
1-aminocyclopropane-1-

carboxylate synthase-like protein

221
60S ribosomal protein L39
1572
putative beta-glucosidase

222
unknown protein
1573
transcription factor ZAP1

223
putative SF16 protein {Helianthus
1574
oligopeptide transporter, putative

annuus}

224
unknown protein
1575
putative protein

225
thioredoxin
1576
putative glucosyltransferase

226
trehalose-6-phosphate phosphatase
1577
putative serine/threonine kinase

(AtTPPB)

227
chlorophyll a/b-binding protein
1578
squalene epoxidase-like protein

228
class IV chitinase (CHIV)
1579
similar to 14 KD proline-rich

protein DC2.15 precursor

(sp|P14009); similar to

ESTs emb|Z17709 and

emb|Z47685

229
chalcone synthase (naringenin-
1580
unknown protein

chalcone synthase) (testa 4 protein)

(sp|P13114)

230
unknown protein
1581
unknown protein

231
cinnamyl-alcohol dehydrogenase
1582
hypothetical protein

ELI3-2

232
farnesyl-pyrophosphate synthetase
1583
60S ribosomal protein L38

FPS2

233
phospholipid hydroperoxide
1584
flavin-containing

glutathione peroxidase

monooxygenase, putative

234
heat shock transcription factor
1585
remorin

HSF4

235
heat shock protein 101
1586
unknown protein

236
17.6 kDa heat shock protein (AA
1587
putative protein

1-156)

237
heat shock protein 17.6A
1588
lipoxygenase

238
heat-shock protein
1589
cold-regulated protein

COR6.6 (KIN2)

239
HY5
1590
Myb transcription factor

homolog (ATR1)

240
putative auxin-induced protein,
1591
putative protein

IAA12

241
early auxin-induced protein,
1592
unknown protein

IAA19

242
auxin-inducible gene (IAA2)
1593
unknown protein

243
putative protein
1594
Ca2+-transporting ATPase-

like protein

244
putative choline kinase
1595
protein phosphatase 2C

(AtP2C-HA)

245
thymidylate kinase-like protein
1596
peroxidase ATP24a

246
CTP synthase like protein
1597
branched-chain alpha keto-

acid dehydrogenase,

putative

247
putative protein
1598
putative beta-ketoacyl-CoA

synthase

248
putative amidase
1599
putative protein

249
4-alpha-glucanotransferase
1600
putative beta-galactosidase

250
hypothetical protein
1601
putative protein

251
similar to auxin-induced protein
1602
60S ribosomal protein L27

252
putative protein
1603
putative annexin

253
putative protein
1604
NAC domain protein,

putative

254
putative protein
1605
unknown protein

255
hyuC-like protein
1606
late embryogenesis

abundant protein LEA like

256
putative tetracycline
1607
unknown protein

transporter protein

257
similar to early nodulins
1608
putative protein

258
putative protein
1609
dehydrin Xero2

259
putative peptidyl-prolyl cis-
1610
putative zinc finger protein

trans isomerase

260
unknown protein
1611
unknown protein

261
unknown protein
1612
DnaJ-like protein

262
putative endochitinase
1613
putative inositol polyphosphate-5-

phosphatase

263
putative ABC transporter
1614
putative cytochrome P450

264
No function assigned by
1615
putative protein

TIGR

265
CONSTANS-like B-box
1616
unknown protein

zinc finger protein

266
unknown protein
1617
putative protein

267
unknown protein
1618
hypothetical protein

268
putative mitochondrial
1619
putative protein

processing peptidase alpha

subunit

269
putative pre-mRNA
1620
sucrose-UDP glucosyltransferase

splicing factor

270
putative phosphatidylserine
1621
glucose-6-phosphate 1-

decarboxylase

dehydrogenase

271
unknown protein
1622
unknown protein

272
unknown protein
1623
mitochondrial chaperonin (HSP60)

273
unknown protein
1624
sucrose transport protein SUC1

274
putative casein kinase I
1625
putative protein disulfide isomerase

275
unknown protein
1626
putative pollen-specific protein

276
60S ribosomal protein
1627
integral membrane protein,

L23A

putative

277
putative mitochondrial
1628
rubredoxin, putative

dicarboxylate carrier

protein

278
enoyl-ACP reductase (enr-
1629
putative protein

A)

279
putative isoamylase
1630
disease resistance protein RPS4,

putative

280
formamidase-like protein
1631
putative peptide/amino acid

transporter

281
reticuline oxidase-like
1632
peroxidase, putative

protein

282
unknown protein
1633
ethylene receptor, putative (ETR2)

283
putative transketolase
1634
protein phosphatase 2C (PP2C)

precursor

284
putative protein
1635
putative glutathione S-transferase

285
unknown protein
1636
homeodomain transcription factor

(ATHB-7)

286
unknown protein
1637
putative nitrate transporter

287
unknown protein
1638
putative ribosomal protein L9,

cytosolic

288
putative esterase D
1639
putative DNA-binding protein

289
predicted protein of unknown
1640
beta-1,3-glucanase-like protein

function

290
unknown protein
1641
putative zinc transporter

291
putative indole-3-glycerol
1642
transcription factor TINY

phosphate synthase

292
isopentenyl
1643
putative aspartate kinase-

pyrophosphate:dimethyllallyl

homoserine dehydrogenase

pyrophosphate isomerase

293
kinase associated protein
1644
ethylene reponse factor-like AP2

phosphatase

domain transcription factor

294
putative K+ channel, beta subunit
1645
peptide transporter-like protein

295
KNAT1 homeobox-like protein
1646
trehalose-6-phosphate synthase like

protein

296
PSI type II chlorophyll a/b-binding
1647
putative ribonuclease

protein, putative

297
transcription factor
1648
hypothetical protein

298
putative WD-40 repeat protein,
1649
putative DNA-binding

MSI2

protein

299
WD-40 repeat protein (MSI3)
1650
nodulin-like protein

300
putative WD-40 repeat protein,
1651
trehalose-6-phosphate

MSI4

phosphatase-like protein

301
unknown protein
1652
succinate dehydrogenase

flavoprotein alpha subunit

(emb|CAA05025.1)

302
hypothetical protein
1653
unknown protein

303
putative protein
1654
stress related protein,

putative

304
No function assigned by TIGR
1655
putative chloroplast

initiation factor 3

305
polyphosphoinositide binding
1656
putative protein

protein, putative

306
hypothetical protein
1657
hypothetical protein

307
unknown protein
1658
putative CCCH-type zinc

finger protein

308
chloroplast ribosomal L1-like
1659
similar to harpin-induced

protein

protein hin1 from tobacco

309
cold-regulated protein cor15b
1660
unknown protein

precursor

310
cyanohydrin lyase like protein
1661
unknown protein

311
putative replication protein A1
1662
hypothetical protein

312
putative protein
1663
No function assigned by

TIGR

313
possible apospory-associated like
1664
putative protein

protein

314
DNA binding protein GT-1,
1665
putative glutathione S-

putative

transferase TSI-1

315
AT-hook DNA-binding protein
1666
putative protein

(AHP1)

316
putative phospholipase
1667
putative PTR2 family

peptide transporter

317
chloroplast FtsH protease, putative
1668
receptor kinase-like protein

318
enoyl-CoA hydratase like
1669
putative sugar transport

protein

protein, ERD6

319
berberine bridge enzyme-
1670
putative protein

like protein

320
putative sugar transporter
1671
nodulin-like protein

321
unknown protein
1672
unknown protein

322
No function assigned by
1673
putative receptor-like

TIGR

protein kinase

323
hypothetical protein
1674
glutathione-conjugate

transporter AtMRP4

324
putative acidic ribosomal
1675
ascorbate oxidase-like

protein

protein

325
putative protein
1676
pathogenesis-related protein 1

precursor, 19.3K

326
unknown protein
1677
R2R3-MYB transcription factor

327
hypothetical protein
1678
hypothetical protein

328
putative protein
1679
putative chitinase

329
dihydroxypolypreny
1680
Mlo protein, putative

lbenzoate methyltransferase

330
unknown protein
1681
putative WRKY-type DNA binding

protein

331
myb-related protein
1682
putative acyl-CoA synthetase

332
No function assigned by
1683
putative pathogenesis-related

TIGR

protein

333
putative protein
1684
putative chitinase

334
putative disease resistance
1685
germin precursor oxalate oxidase

response protein

335
hypothetical protein
1686
endoxyloglucan transferase,

putative

336
No function assigned by
1687
putative protein

TIGR

337
starch branching enzyme II
1688
putative cytochrome P450

338
No function assigned by
1689
similar to Mlo proteins from H.

TIGR

vulgare

339
putative enolase (2-
1690
putative tropinone reductase

phospho-D-glycerate

hydroylase)

340
putative protein kinase
1691
extensin-like protein

341
HD-Zip protein, putative
1692
putative sarcosine oxidase

342
putative protein kinase
1693
putative protein

343
phenylalanyl-trna
1694
hypothetical protein

synthetase-like protein

344
putative aconitase
1695
late embryogenesis-abundant

protein, putative

345
NAM (no apical meristem)
1696
beta-carotene hydroxylase

protein, putative

346
unknown protein
1697
putative calcium binding protein

347
putative
1698
unknown protein

phosphomannomutase

348
putative farnesylated protein
1699
unknown protein

349
unknown protein
1700
predicted glycosyl transferase

350
water stress-induced protein,
1701
hypothetical protein

putative

351
unknown protein
1702
hypothetical protein

352
unknown protein
1703
hypothetical protein

353
PEROXISOMAL MEMBRANE
1704
putative protein

PROTEIN PMP22

354
putative peroxisomal membrane
1705
unknown protein

carrier protein

355
putative protein
1706
putative protein

356
unknown protein
1707
putative protein

357
putative protein
1708
serine/threonine kinase-like

protein

358
putative protein
1709
No function assigned by TIGR

359
argininosuccinate synthase-like
1710
putative pectinesterase

protein

360
1-phosphatidylinositol-4,5-
1711
peroxidase like protein

bisphosphate phosphodiesterase

361
putative JUN kinase activator
1712
No function assigned by TIGR

protein

362
putative 60S ribosomal protein L35
1713
phenylalanine ammonia

lyase (PALI)

363
nucleoid DNA-binding protein
1714
peroxidase

cnd41-like protein

(emb|CAA68212.1)

364
SigA binding protein
1715
putative AMP deaminase

365
hypothetical protein
1716
putative MYB family

transcription factor

366
putative protein kinase
1717
DNA-directed RNA

polymerase II, third largest subunit

367
unknown protein
1718
nucleotide pyrophosphatase-

like protein

368
regulatory protein NPR1-like;
1719
putative peroxidase

transcription factor inhibitor I

kappa B-like

369
putative protein
1720
calcium sensor homolog

(gb|AAC26110.1)

370
hypothetical protein
1721
putative GDSL-motif

lipase/hydrolase

371
phosphoribosylanthranilate
1722
putative nonspecific lipid-

isomerase

transfer protein

372
phosphoribosylanthranilate
1723
acyl-carrier protein (ACP),

isomerase

putative

373
sterol glucosyltransferase, putative
1724
putative glycine

dehydrogenase

374
putative gigantea protein
1725
AIG1

375
putative MYB family transcription
1726
ACC synthase (AtACS-6)

factor

376
hypothetical protein
1727
cyclin delta-3

377
hypothetical protein
1728
putative RING zinc finger

protein

378
predicted protein
1729
aldose 1-epimerase-like

protein

379
cytochrome P450, putative
1730
putative phospholipase

380
putative Na+ dependent
1731
phosphoenolpyruvate

ileal bile acid transporter

carboxylase

381
unknown protein
1732
putative galactinol synthase

382
RING-H2 finger protein
1733
unknown protein

RHF1a

383
putative protein
1734
putative protein

384
unknown protein
1735
1-aminocyclopropane-1-

carboxylate oxidase

385
putative protein
1736
thioredoxin (clone GIF1)

(pir||S58118)

386
putative auxin-regulated
1737
trehalose-6-phosphate

protein

phosphatase

387
hypothetical protein
1738
beta-1,3-glucanase 2 (BG2)

(PR-2)

388
unknown protein
1739
putative S-adenosyl-L-

methionine:trans-caffeoyl-

Coenzyme A 3-O-

methyltransferase

389
unknown protein
1740
disease resistance protein EDS1

390
putative protein
1741
putative protein kinase

391
putative protein
1742
Gluthatione reductase, chloroplast

precursor

392
unknown protein
1743
putative heat shock protein

393
histone H1
1744
aspartate kinase

394
Argonaute (AGO1)-like
1745
putative major intrinsic (channel)

protein

protein

395
unknown protein
1746
matrix metalloproteinase, putative

396
putative protein with C-
1747
putative GDSL-motif

terminal RING finger

lipase/hydrolase

397
unknown protein
1748
putative protein

398
unknown protein
1749
DAG-like protein

399
unknown protein
1750
serine/threonine kinase-like

protein

400
unknown protein
1751
formamidase-like protein

401
unknown protein
1752
CER2

402
putative copper amine
1753
26S proteasome subunit 4

oxidase

403
unknown protein
1754
pectinesterase like protein

404
unknown protein
1755
putative disease resistance protein

405
unknown protein
1756
putative RNA methyltransferase

406
putative protein
1757
unknown protein

407
putative protein
1758
HOMEOBOX PROTEIN

KNOTTED-1 LIKE 4 (KNAT4)

408
unknown protein
1759
glycine-rich RNA-binding protein

AtGRP2-like

409
unknown protein
1760
putative acetylornithine

transaminase

410
putative protein
1761
putative Sec24-like COPII protein

411
putative protein
1762
putative berberine bridge enzyme

412
unknown protein
1763
putative GH3-like protein

413
serine/threonine kinase-
1764
putative ABC transporter

like protein

414
alcohol dehydrogenase,
1765
putative reticuline oxidase-like

putative

protein

415
anthranilate
1766
pectate lyase-like protein

phosphoribosyltransferase,

chloroplast precursor (sp|Q02166)

416
phytochrome C (sp|P14714)
1767
protein disulfide-isomerase-like

protein

417
putative phytochrome-associated
1768
putative protein

protein 3

418
receptor serine/threonine kinase
1769
putative membrane transporter

PR5K

419
Ran-binding protein (atranbp1a)
1770
unknown protein

420
small Ras-like GTP-binding
1771
unknown protein

protein (gb|AAB58478.1)

421
sterol-C5-desaturase
1772
putative RING-H2 zinc finger

protein

422
tryptophan synthase beta chain 1
1773
unknown protein

precursor (sp|P14671)

423
thioredoxin f2 (gb|AAD35004.1)
1774
unknown protein

424
No function assigned by TIGR
1775
unknown protein

425
putative WRKY DNA-binding
1776
MADS-box protein

protein

(AGL20)

426
putative protein
1777
amidophosphoribosyltransfer-

ase 2 precursor

427
unknown protein
1778
putative dihydrodipicolinate

synthase

428
unknown protein
1779
hypothetical protein

429
14-3-3 protein homolog RCI1
1780
ABA-responsive protein-

(pir||S47969)

like

430
unknown protein
1781
putative protein

431
putative CCCH-type zinc finger
1782
hypothetical protein

protein

432
PINHEAD (gb|AAD40098.1);
1783
DNA-binding protein-like

translation initiation factor

433
plasma membrane proton ATPase
1784
No function assigned by

(PMA)

TIGR

434
CHLOROPHYLL A-B BINDING
1785
transcription factor,

PROTEIN 4 PRECURSOR

putative

homolog

435
membrane related protein CP5,
1786
nitrate reductase, putative

putative

436
ABC transporter (AtMRP2)
1787
putative protein

437
putative embryo-abundant protein
1788
putative protein

438
putative anthocyanidin-3-glucoside
1789
putative protein

rhamnosyltransferase

439
putative lipid transfer protein
1790
putative protein

440
unknown protein
1791
unknown protein

441
unknown protein
1792
unknown protein

442
galactinol synthase, putative
1793
tryptophan synthase beta-

subunit (TSB2)

443
putative protein
1794
hypothetical protein

444
putative protein
1795
putative protein

445
SCARECROW-like protein
1796
putative DNA-binding

protein

446
unknown protein
1797
putative 40S ribosomal

protein S10

447
unknown protein
1798
putative protein

448
unknown protein
1799
putative cytochrome P450

449
unknown protein
1800
putative protein

450
asparagine--tRNA ligase
1801
putative protein

451
putative protein
1802
putative glucosyltransferase

452
glutamate-1-semialdehyde
1803
No function assigned by

2,1-aminomutase 1

TIGR

precursor (GSA 1)

(glutamate-1-semialdehyde

aminotransferase 1) (GSA-

AT 1) (sp|P42799)

453
hypothetical protein
1804
putative protein

454
putative serine protease-like
1805
putative protein

protein

455
No function assigned by
1806
unknown protein

TIGR

456
unknown protein
1807
glycine-rich RNA binding protein

7

457
unknown protein
1808
dehydrin, putative

458
gamma-adaptin, putative
1809
putative endoxyloglucan

glycosyltransferase

459
UDP rhamnose--
1810
glutamate decarboxylase 1 (GAD

anmocyanidin-3-glucoside

1) (sp|Q42521)

rhamnosyltransferase-like

protein

460
carbonate dehydratase-like
1811
delta 9 desaturase

protein

461
putative microtubule-
1812
UDP-glucose glucosyltransferase

associated protein

462
putative ribophorin I
1813
CARBONIC ANHYDRASE 2

463
putative zinc finger protein
1814
response reactor 2 (ATRR2)

464
chloroplast FtsH protease,
1815
S-adenosyl-methionine-sterol-C-

putative

methyltransferase, putative

465
putative protein
1816
putative DNA-binding protein

(RAV2-like)

466
unknown protein
1817
gamma glutamyl hydrolase,

putative

467
putative LEA protein
1818
protein phosphatase-like

468
putative protein
1819
unknown protein

469
putative protein
1820
unknown protein

470
unknown protein
1821
unknown protein

471
putative purple acid
1822
copper transport protein-like

phosphatase

protein

472
unknown protein
1823
hypothetical protein

473
putative protein
1824
unknown protein

474
unknown protein
1825
putative peptide methionine

sulfoxide reductase

475
chlorophyll binding protein,
1826
putative obtusifoliol 14-alpha

putative

demethylase

476
phosphoenolpyruvate carboxylase
1827
glutamate dehydrogenase (EC

(PPC)

1.4.1.-) 1 (pir||S71217)

477
chlorophyll a/b-binding protein-
1828
unknown protein

like

478
AtAGP4
1829
xyloglucan endo-1,4-beta-D-

glucanase precursor

479
putative cryptochrome 2 apoprotein
1830
unknown protein

480
type 2 peroxiredoxin, putative
1831
SNF1 related protein kinase

(ATSRPK1)

481
Atpm24.1 glutathione S transferase
1832
putative protein

482
delta tonoplast integral protein
1833
putative chloroplast nucleoid DNA

(delta-TIP)

binding protein

483
20S proteasome subunit (PAA2)
1834
hypothetical protein

484
dormancy-associated protein,
1835
putative protein

putative

485
putative cytidine deaminase
1836
putative thiamin biosynthesis

protein

486
No function assigned by TIGR
1837
unknown protein

487
putative phospholipase D-gamma
1838
unknown protein

488
cell elongation protein, Dwarf1
1839
putative RNA helicase

489
germin-like protein
1840
putative SF21 protein

{Helianthus annuus}

490
hevein-like protein precursor (PR-
1841
unknown protein

4)

491
rac-like GTP binding protein
1842
NBS/LRR disease

(ARAC5)

resistance protein, putative

492
phosphoprotein phosphatase, type
1843
hypothetical protein

1 catalytic subunit

493
ubiquitin-protein ligase UBC9
1844
unknown protein

494
xyloglucan endotransglycosylase-
1845
No function assigned by

related protein XTR-7

TIGR

495
cysteine synthase
1846
glycine-rich protein

(AtGRP2)

496
putative villin 2
1847
No function assigned by

TIGR

497
glutathione S-transferase
1848
putative protein

498
5-adenylylsulfate reductase
1849
putative glucosyltransferase

499
arginine decarboxylase
1850
hypothetical protein

500
ATHP2, putative
1851
hypothetical protein

501
ornithine carbamoyltransferase
1852
putative protein

precursor

502
puative protein
1853
putative disease resistance

protein

503
putative protein
1854
thaumatin, putative

504
unknown protein
1855
putative proline-rich protein

505
putative protein
1856
sterol-C-methyltransferase

506
putative protein
1857
superoxidase dismutase

507
unknown protein
1858
TINY-like protein

508
unknown protein
1859
calcium-dependent protein

kinase, putative

509
unknown protein
1860
hypothetical protein

510
unknown protein
1861
putative protein kinase

511
hypothetical protein
1862
DNA-directed RNA

polymerase (mitochondrial)

512
putative protein
1863
putaive DNA-binding

protein

513
putative DnaJ protein
1864
late embryogenesis

abundant M17 protein

514
plastocyanin
1865
putative protein

515
unknown protein
1866
delta-1-pyrroline-5-

carboxylate synthetase

516
unknown protein
1867
putative 60s ribosomal

protein L10

517
unknown protein
1868
cytochrome P450

CYP86A1

518
unknown protein
1869
putative tyrosine aminotransferase

519
unknown protein
1870
thionin

520
unknown protein
1871
No function assigned by TIGR

521
putative ATP-dependent
1872
APETALA2 protein

RNA helicase

522
non-race specific disease
1873
MADS-box protein (AGL3)

resistance protein (NDR1)

523
hypothetical protein
1874
putative monooxygenase

524
putative protein
1875
ZFP3 zinc finger protein

525
putative protein
1876
cell division protein FtsZ

chloroplast homolog precursor

(sp|Q42545)

526
putative protein
1877
calreticulin, putative

527
copper transport protein
1878
phosphoserine aminotransferase

528
putative protein
1879
12-oxophytodienoate-10,11-

reductase

529
unknown protein
1880
putative bHLH transcription factor

530
unknown protein
1881
pectin methylesterase (PMEU1),

putative

531
unknown protein
1882
DNA-binding protein

532
putative protein kinase
1883
carnitine racemase like protein

533
unknown protein
1884
putative protein

534
putative protein
1885
endoxyloglucan transferase

(dbj|BAA81669.1)

535
putative protein
1886
RMA1 RING zinc finger protein

536
hypothetical protein
1887
ammonium transporter

537
putative protein
1888
apyrase (gb|AAF00612.1)

538
putative AP2 domain
1889
potassium uptake transporter-like

transcription factor

protein

539
putative nitrilase
1890
putative ABC transporter

540
putative protein
1891
potassium transporter-like protein

541
putative tetrahydrofolate
1892
integral membrane protein,

synthase

putative

542
heat-shock protein
1893
putative protein

543
unknown protein
1894
pyruvate decarboxylase-1 (Pdc1)

544
unknown protein
1895
putative malate oxidoreductase

545
histone H4
1896
putative histone H2B

546
hypothetical protein
1897
snoRNA

547
unknown protein
1898
symbiosis-related like protein

548
putative protein
1899
unknown protein

549
predicted protein
1900
unknown protein

550
putative dihydrolipoamide
1901
hypothetical protein

succinyltransferase

551
actin 3
1902
putative protein

552
putative CCCH-type zinc finger
1903
copper-binding protein-like

protein

553
MAP kinase kinase 2
1904
putative protein

554
ethylene-insensitive3-like1 (EIL1)
1905
unknown protein

555
histidine transport protein (PTR2-
1906
putative glyoxalase II

B)

556
putative auxin-induced protein
1907
No function assigned by

AUX2-11

TIGR

557
hydroxyacylglutathione hydrolase
1908
hypothetical protein

cytoplasmic (glyoxalase II) (GLX

II)

558
delta-8 sphingolipid desaturase
1909
flavanone 3-hydroxylase

(FH3)

559
cellulose synthase catalytic subunit
1910
putative laccase

(Ath-A)

560
nitrate transporter (NTL1)
1911
putative protein kinase

561
DNA-binding homeotic protein
1912
myb-related protein, 33.3K

Athb-2

(pir||S71284)

562
hypothetical protein
1913
unknown protein

563
aspartate aminotransferase
1914
endo-xyloglucan transferase-

like protein

564
4-coumarate:CoA ligase 1
1915
TMV resistance protein N-

like

565
pyruvate dehydrogenase E1 beta
1916
putative xyloglucan

subunit, putative

endotransglycosylase

566
nucleotide diphosphate kinase Ia
1917
unknown protein

(emb|CAB58230.1)

567
chloroplast Cpn21 protein
1918
proline transporter 2

568
ATP dependent copper transporter
1919
resistance protein, putative

569
very-long-chain fatty acid
1920
actin, putative

condensing enzyme (CUT1)

570
putative purine-rich single-stranded
1921
putative related to microbial

DNA-binding protein

divalent cation tolerance

proteins

571
serine/threonine protein
1922
unknown protein

phosphatase (type 2A)

572
isopentenyl
1923
putative glycosyl

diphosphate:dimethylallyl

transferase

diphosphate isomerase (IPP2)

573
putative c2h2 zinc finger
1924
unknown protein

transcription factor

574
putative 20S proteasome beta
1925
putative protein

subunit PBC2

phosphatase 2C

575
nucleoside diphosphate kinase 3
1926
unknown protein

(ndpk3)

576
ras-related small GTP-binding
1927
serpin, putative

protein

577
putative 4-coumarate:CoA ligase 2
1928
cinnamyl-alcohol

dehydrogenase CAD1

578
transcription factor HBP-1b
1929
putative protein import

homolog (sp|P43273)

receptor

579
biotin synthase (Bio B)
1930
unknown protein

580
homeobox protein HAT22
1931
unknown protein

581
putative preprotein
1932
putative protein

translocase SECY protein

582
carbamoylphosphate
1933
putative CDP-

synthetase, putative

diacylglycerol--glycerol-3-

phosphate 3-

phosphatidyltransferase

583
putative protein kinase,
1934
unknown protein

ADK1

584
putative nuclear DNA-
1935
putative LRR receptor-like

binding protein G2p

protein kinase

585
hypothetical protein
1936
serine/threonine protein kinase,

putative

586
hypothetical protein
1937
potassium transporter-like protein

587
unknown protein
1938
lactate dehydrogenase (LDH1)

588
unknown protein
1939
hypothetical protein

589
molybdopterin synthase
1940
unknown protein

(CNX2)

590
putative ribosomal protein
1941
putative thaumatin

L6

591
unknown protein
1942
putative reticuline oxidase-like

protein

592
En/Spm-like transposon
1943
uracil phosphoribosyltransferase,

protein

putative

593
putative protein
1944
transcription factor, putative

594
putative protein
1945
unknown protein

595
unknown protein
1946
unknown protein

596
hypothetical protein
1947
GATA transcription factor 4

597
unknown protein
1948
unknown protein

598
unknown protein
1949
unknown protein

599
putative lysosomal acid
1950
senescence-associated protein-like

lipase

600
unknown protein
1951
putative pollen allergen

601
unknown protein
1952
unknown protein

602
NifS-like aminotranfserase
1953
putative protein

603
actin 8
1954
glycine-rich protein

604
hypothetical protein
1955
putative protein

605
putative protein
1956
3-methyladenine DNA glycosylase,

putative

606
heat-shock protein (At-
1957
endoplasmic reticulum-type

hsc70-3)

calcium-transporting ATPase 4

607
putative protein disulfide
1958
putative pectinesterase

isomerase precursor

608
adenosine nucleotide
1959
cytochrome P450-like protein

translocator

609
photosystem II oxygen-evolving
1960
RNA-binding protein (cp33)

complex protein 3-like

610
sedoheptulose-bisphosphatase
1961
CONSTANS-like 1

precursor

611
glutathione S-transferase (GST6)
1962
putative small heat shock protein

612
geranylgeranyl reductase
1963
hypothetical protein

613
hypothetical protein
1964
unknown protein

614
hypothetical protein
1965
cytochrome P450-like protein

615
phosphoribulokinase precursor
1966
cysteine proteinase inhibitor like

protein

616
high mobility group protein
1967
nicotianamine synthase

(HMG1), putative

(dbj|BAA74589.1)

617
protease inhibitor II
1968
copper amine oxidase like protein

(fragment2)

618
protease inhibitor II
1969
putative SCARECROW gene

regulator

619
cytochrome P450 90A1
1970
unknown protein

(sp|Q42569)

620
unknown protein
1971
unknown protein

621
heat shock protein 90
1972
putative alanine acetyl

transferase

622
tubulin beta-9 chain
1973
unknown protein

623
putative ubiquitin carboxyl
1974
unknown protein

terminal hydrolase

624
protein kinase
1975
unknown protein

625
DRE/CRT-binding protein
1976
putative extensin

DREB1C

626
histidyl-tRNA synthetase
1977
putative protein kinase

627
splicing factor, putative
1978
putative protein kinase

628
glutamyl-tRNA synthetase
1979
NADPH-dependent

codeinone reductase,

putative

629
putative RING zinc finger protein
1980
peroxidase

630
phytochelatin synthase
1981
putative cytochrome P450

(gb|AAD41794.1)

631
putative C2H2-type zinc finger
1982
No function assigned by

protein

TIGR

632
putative ligand-gated ion channel
1983
putative zinc-finger protein

protein

(B-box zinc finger domain)

633
putative ribosomal-protein S6
1984
putative tyrosine

kinase (ATPK6)

aminotransferase

634
MOLYBDOPTERIN
1985
hypothetical protein

BIOSYNTHESIS CNX1

PROTEIN

635
temperature-sensitive omega-3
1986
DNA binding protein

fatty acid desaturase, chloroplast

precursor (sp|P48622)

636
adenylosuccinate synthetase
1987
putative fatty acid elongase

637
putative 14-3-3 protein
1988
bZIP transcription factor-

like protein

638
putative cytochrome P450
1989
xyloglucan

fucosyltransferase, putative

639
putative two-component
1990
unknown protein

response regulator 3 protein

640
putative RING-H2 zinc
1991
unknown protein

finger protein ATL6

641
No function assigned by
1992
putative protein

TIGR

642
small zinc finger-like
1993
myb factor, putative

protein

643
hypothetical protein
1994
Myb-family transcription

factor, putative

644
MAP kinase (ATMPK6)
1995
putative fructose

bisphosphate aldolase

645
vacuolar ATP synthase,
1996
myrosinase-associated

putative

protein, putative

646
kinesin-like protein
1997
cytochrome P450 like

protein

647
serine/threonine-specific
1998
similar to SOR1 from the

protein kinase NAK

fungus Cercospora

nicotianae

648
No function assigned by
1999
similar to embryo-abundant

TIGR

protein GB:L47672 GI:1350530

[Picea glauca]

649
ACTIN 2/7 (sp|P53492)
2000
alcohol dehydrogenase

650
phosphoglycerate kinase,
2001
auxin response factor 1

putative

651
homeotic protein BEL1
2002
pathogenesis-related protein 1

homolog

precursor, 18.9K

652
proline iminopeptidase
2003
hypothetical protein

653
pasticcino 1
2004
unknown protein

654
serine/threonine protein
2005
zinc finger protein Zat12

kinase

655
cytochrome P450
2006
unknown protein

monooxygenase

(CYP71B4)

656
No function assigned by
2007
unknown protein

TIGR

657
putative GDSL-motif
2008
cyclin, putative

lipase/hydrolase

658
putative protein
2009
2-dehydro-3-

deoxyphosphoheptonate aldolase

659
unknown protein
2010
glutathione synthetase gsh2

660
hypothetical protein
2011
heat shock protein 17

661
putative glycosylation
2012
putative Na+-dependent inorganic

enzyme

phosphate cotransporter

662
No function assigned by
2013
No function assigned by TIGR

TIGR

663
No function assigned by
2014
unknown protein

TIGR

664
unknown protein
2015
putative protein

665
putative ABC transporter
2016
similar to RING-H2 finger protein

RHC1a GB:AAC69854

GI:3790583 from [Arabidopsis

thaliana]

666
nifU-like protein
2017
calcium-binding protein-like

667
putative receptor-like protein
2018
putative protein

kinase

668
putative disease resistance protein
2019
putative aldehyde dehydrogenase

669
receptor-like protein kinase-like
2020
auxin-responsive GH3-like

protein

670
ubiquitin activating enzyme 2
2021
putative protein

(gb|AAB37569.1)

671
No function assigned by TIGR
2022
Phosphoglycerate dehydrogenase-

like protein

672
putative receptor-like protein
2023
unknown protein

kinase

673
K+ transporter, AKT1
2024
unknown protein

674
shaggy-like kinase beta
2025
PSI type III chlorophyll a/b-

binding protein, putative

675
heat shock protein 70
2026
putative protein

676
plasma membrane intrinsic protein
2027
putative protein

1a

677
HSP90-like protein
2028
glutaredoxin, putative

678
histone H1, putative
2029
hypothetical protein

679
unknown protein
2030
No function assigned by TIGR

680
dnaK-type molecular chaperone
2031
putative protein

hsc70.1-like

681
gamma-glutamylcysteine
2032
jasmonate inducible protein,

synthetase

putative

682
peroxidase (ATP22a)
2033
putative polygalacuronase

isoenzyme 1 beta subunit

683
putative serine carboxypeptidase
2034
putative small heat shock protein

precursor

684
putative dioxygenase
2035
unknown protein

685
glucose transporter
2036
putative disease resistance

protein

686
NOI protein, nitrate-induced
2037
putative protein

687
putative protein
2038
ethylene-responsive

element binding factor,

putative

688
putative protein
2039
putative protein

689
unknown protein
2040
Pollen-specific protein

precursor like

690
putative photosystem I reaction
2041
putative protein

center subunit II precursor

691
putative protein
2042
unknown protein

692
unknown protein
2043
EF-Hand containing protein-

like

693
cobalamin biosynthesis protein
2044
unknown protein

694
adenine nucleotide translocase
2045
puative calcium-

transporting ATPase

695
glutathione transferase, putative
2046
antifungal protein-like

(PDF1.2)

696
putative 60S ribosomal protein L21
2047
pathogenesis-related PR-1-

like protein

697
cytochrome P450 like protein
2048
similar to Mlo proteins

from H. vulgare

698
cytochrome b245 beta chain
2049
putative steroid

homolog RbohAp108, putative

sulfotransferase

699
RNA helicase, DRH1
2050
trehalase-like protein

700
putative aldolase
2051
thioredoxin f1

701
farnesyltransferase subunit A
2052
unknown protein

(FTA)

702
No function assigned by
2053
alanine-glyoxylate

TIGR

aminotransferase

703
putative putative sister-
2054
integral membrane protein,

chromatide cohesion

putative

protein

704
calcium-dependent protein
2055
hypothetical protein

kinase

705
serine/threonine protein
2056
unknown protein

phosphatase type 2A,

putative

706
40S ribosomal protein S28
2057
hypothetical protein

(sp|P34789)

707
RNA polymerase subunit
2058
unknown protein

708
DNA-damage-
2059
unknown protein

repair/toleration protein

DRT102

709
putative C2H2-type zinc
2060
unknown protein

finger protein

710
putative adenosine
2061
drought-induced-19-like 1

phosphosulfate kinase

711
lipase
2062
unknown protein

712
putative violaxanthin de-
2063
putative protein

epoxidase precursor

(U44133)

713
aromatic rich glycoprotein,
2064
putative protein

putative

714
putative fumarase
2065
AIG2-like protein

715
flavonol synthase (FLS)
2066
Lhca2 protein

(sp|Q96330)

716
response regulator 5,
2067
phytocyanin

putative

717
sulfate transporter
2068
putative chlorophyll A-B binding

protein

718
putative floral homeotic
2069
Lhcb3 chlorophyll a/b binding

protein, AGL9

protein (gb|AAD28773.1)

719
putative ethylene-inducible
2070
luminal binding protein

protein

(dbj|BAA13948.1)

720
C-8,7 sterol isomerase
2071
hydroxypyruvate reductase (HPR)

721
TCH4 protein
2072
epoxide hydrolase (ATsEH)

(gb|AAA92363.1)

722
hypothetical protein
2073
putative protein (fragment)

723
putative urease accessory
2074
unknown protein

protein

724
molybdopterin synthase
2075
hypothetical protein

sulphurylase

(gb|AAD18050.1)

725
putative protein
2076
putative glucosyl transferase

726
NBD-like protein
2077
putative glucosyl transferase

(gb|AAD20643.1)

727
AtHVA22c
2078
putative 3-methylcrotonyl-CoA

carboxylase

728
unknown protein
2079
putative peroxidase

729
phytoene synthase
2080
acyl-CoA oxidase

(gb|AAB65697.1)

(gb|AAC13497.1)

730
protein kinase (AME2/AFC1)
2081
alternative oxidase 1a precursor

731
hypothetical protein
2082
putative transcription factor

(MYB4)

732
cyclin-dependent protein kinase-
2083
serine acetyltransferase

like protein

733
photosystem II stability/assembly
2084
ATP-sulfurylase

factor HCF136 (sp|O82660)

734
hypothetical protein
2085
calreticulin (crt1)

735
DNA binding-like protein
2086
putative prohibitin 2

736
putative protein
2087
putative monodehydroascorbate

reductase

737
chorismate mutase
2088
branched-chain alpha-keto acid

decarboxylase E1 beta subunit

738
putative LRR receptor protein
2089
cytokinin oxidase-like protein

kinase

739
putative chalcone synthase
2090
putative receptor-like protein

kinase

740
putative protein kinase
2091
unknown protein

741
replicase, putative
2092
hypothetical protein

742
putative cysteine proteinase
2093
No function assigned by TIGR

743
60S ribosomal protein L36
2094
putative APG protein

744
unknown protein
2095
glutathione S-transferase, putative

745
CLC-b chloride channel protein
2096
phytochrome-associated protein 1

(PAP1)

746
putative ribosomal protein S14
2097
amidophosphoribosyltransferase

747
histone H2B like protein
2098
nonphototropic hypocotyl 1

(emb|CAA69025.1)

748
60S ribosomal protein L2
2099
3-keto-acyl-CoA thiolase 2

(gb|AAC17877.1)

749
60S ribosomal protein L15
2100
pEARLI 1

homolog

750
ribosomal protein S27
2101
glutathione reductase, cytosolic

751
ribosomal protein
2102
putative protein

752
60S ribosomal protein L12
2103
putative protein

753
60s ribosomal protein L34
2104
putative aldehyde oxidase

754
putative ribosomal protein S10
2105
probable photosystem I

chain XI precursor

755
drought-induced protein like
2106
photosystem II polypeptide,

putative

756
blue copper-binding protein, 15K
2107
photosystem II reaction

(lamin)

center 6.1 KD protein

757
calmodulin-like protein
2108
33 kDa polypeptide of

oxygen-evolving complex

(OEC) in photosystem II

(emb|CAA75629.1)

758
putative protein
2109
60S ribosomal protein

L11B

759
No function assigned by TIGR
2110
extA (emb|CAA47807.1)

760
alpha-mannosidase, putative
2111
zinc finger protein OBP4-

like

761
uncoupling protein (ucp/PUMP)
2112
sterol delta7 reductase

762
homeodomain-like protein
2113
putative RAS-related

protein, RAB11C

763
ribosomal protein S18,
2114
glucosyltransferase like

putative

protein

764
similar to SOR1 from the
2115
zinc finger protein (PMZ),

fungus Cercospora

putative

nicotianae

765
60S ribosomal protein L13,
2116
6,7-dimethyl-8-

BBC1 protein

ribityllumazine synthase

precursor

766
50S ribosomal protein L24,
2117
putative protein

chloroplast precursor

767
putative ribosomal protein
2118
osmotin precursor

768
unknown protein
2119
No function assigned by

TIGR

769
aspartate aminotransferase
2120
ferredoxin precusor isolog

(AAT1)

770
potassium channel protein
2121
GH3 like protein

AtKC

771
unknown protein
2122
non-specific lipid transfer

protein

772
peroxisomal targeting
2123
homeodomain transcription

signal type 2 receptor

factor (HAT9)

773
putative protein
2124
putative cytochrome P450

monooxygenase

774
Ras-related GTP-binding
2125
putative protein kinase

protein (ARA-4)

775
S-receptor kinase homolog
2126
putative protein

2 precursor

776
pathogenesis-related group
2127
glyceraldehyde-3-

5 protein, putative

phosphate dehydrogenase

777
Nitrilase 4 (sp|P46011)
2128
putative protein disulfide-

isomerase

778
biotin carboxyl carrier
2129
unknown protein

protein of acetyl-CoA

carboxylase precursor

(BCCP) (sp|Q42533)

779
photosystem I reaction
2130
beta-1,3-glucanase class I

centre subunit psaN

precursor

precursor (PSI-N)

(sp|P49107)

780
3(2),5-bisphosphate
2131
homeobox-leucine zipper protein

nucleotidase

HAT5 (HD-ZIP protein 5) (HD-

ZIP protein ATHB-1)

781
high affinity Ca2+
2132
putative cyclic nucleotide-

antiporter

regulated ion channel protein

782
putative cytoskeletal
2133
P II nitrogen sensing protein GLB I

protein

783
putative peroxidase
2134
H-protein promoter binding factor-

1 (gb|AAC24592.1)

784
respiratory burst oxidase
2135
GAST1-like protein

protein

785
beta-glucosidase
2136
cytochrome P450 GA3

786
calcium-dependent protein kinase
2137
putative protein

(pir||S71196)

787
phosphoinositide specific
2138
Myb-related transcription factor-

phospholipase C

like protein

788
similarity to S-domain receptor-
2139
putative phloem-specific lectin

like protein kinase, Zea mays

789
mitosis-specific cyclin 1b
2140
protein kinase-like protein

790
4-coumarate:CoA ligase 3
2141
unknown protein

791
transcription factor IIB (TFIIB)
2142
SCARECROW transcriptional

regulator-like

792
unknown protein
2143
unknown protein

793
hypothetical protein
2144
unknown protein

794
hypothetical protein
2145
putative protein

795
sugar transporter like protein
2146
calnexin homolog

796
putative trypsin inhibitor
2147
PP1/PP2A phosphatases

pleiotropic regulator PRL2

797
unknown protein
2148
xyloglucan endotransglycosylase,

putative

798
putative multispanning membrane
2149
putative calmodulin

protein

799
receptor-like kinase, putative
2150
spermine synthase (ACL5)

800
putative inosine-5-monophosphate
2151
snoRNA

dehydrogenase

801
inosine-5′-monophosphate
2152
photosystem I subunit V precursor,

dehydrogenase, putative

putative

802
amino acid permease 6
2153
putative potassium transporter

(emb|CAA65051.1)

803
NADPH-ferrihemoprotein
2154
Homeodomain-like protein

reductase (ATR2)

804
putative WRKY-type DNA binding
2155
putative protein

protein

805
putative ankyrin
2156
unknown protein

806
putative hexose transporter
2157
CALMODULIN-RELATED

PROTEIN 2, TOUCH-INDUCED

(TCH2)

807
aquaporin/MIP-like protein
2158
putative protein phosphatase 2C

808
Ser/Thr protein kinase isolog
2159
monosaccharide transport

protein, STP4

809
pectate lyase like protein
2160
hypothetical protein

810
putative 60S ribosomal protein L17
2161
unknown protein

811
putative protein
2162
hypothetical protein

812
unknown protein
2163
putative protein kinase

813
phenylalanine ammonia-lyase
2164
putative serine/threonine

protein kinase

814
putative cytochrome P450
2165
jasmonate inducible

monooxygenase

protein, putative

815
ARR1 protein, putative
2166
similar to several small

proteins (~100 aa) that are

induced by heat, auxin,

ethylene and wounding

such as Phaseolus aureus

indole-3-acetic acid

induced protein ARG

(SW: 32292)

816
putative bHLH transcription factor
2167
unknown protein

817
aminomethyltransferase-like
2168
MYB-like protein

precursor protein

818
purple acid phosphatase precursor
2169
putative protein kinase

819
AP2 domain containing
2170
unknown protein

protein, putative

820
ubiquitin-conjugating
2171
CLC-d chloride channel

enzyme E2-21 kD 1

protein

(ubiquitin-protein ligase 4)

(ubiquitin carrier protein 4)

(sp|P42748)

821
translation initiation factor
2172
cytochrome P450-like

protein

822
putative VAMP-associated
2173
putative glutathione S-

protein

transferase

823
spermidine synthase,
2174
putative mandelonitrile

putative

lyase

824
putative protein
2175
hypothetical protein

825
unknown protein
2176
putative trypsin inhibitor

826
AtKAP alpha
2177
male sterility 2-like protein

(emb|CAA68191.1)

827
glyceraldehyde-3-
2178
unknown protein

phosphate dehydrogenase,

putative

828
putative poly(A) binding
2179
unknown protein

protein

829
alpha-tubulin, putative
2180
putative protein

830
serine/threonine-specific
2181
putative peroxidase

protein kinase ATPK64

(pir||S20918)

831
putative aspartate-tRNA
2182
putative thromboxane-A

ligase

synthase

832
ras-related small GTP-
2183
putative cytochrome P450

binding protein RAB1c

833
cycloartenol synthase
2184
peroxidase ATP21a

834
No function assigned by
2185
unknown protein

TIGR

835
cytochrome P450
2186
putative glutathione S-

transferase

836
GTPase AtRAB8
2187
defender against cell death protein

837
3-phosphoserine
2188
AP2 domain containing protein,

phosphatase

putative

838
transcription factor CRC
2189
actin depolymerizing factor-like

protein

839
nuclear cap-binding
2190
putative calcium-dependent protein

protein; CBP20

kinase (U90439)

(gb|AAD29697.1)

840
chloroplast membrane
2191
phosphoribosylanthranilate

protein (ALBINO3)

transferase, putative

841
biotin holocarboxylase
2192
oligopeptide transporter, putative

synthetase

842
expansin AtEx6
2193
calmodulin-like protein

843
unknown protein
2194
putative protease inhibitor

844
mercaptopyruvate
2195
MAP kinase

sulfurtransferase, putative

845
putative thiosulfate
2196
DNA binding protein MybSt1,

sulfurtransferase

putative

846
dihydrolipoamide S-
2197
putative protein

acetyltransferase

847
auxin transport protein REH1,
2198
putative protein

putative

848
putative auxin transport protein
2199
unknown protein

849
apyrase (Atapy1)
2200
unknown protein

850
root cap 1 (RCP1)
2201
unknown protein

851
hypothetical protein
2202
putative protein

852
putative protein
2203
unknown protein

853
predicted protein of unknown
2204
unknown protein

function

854
hypothetical protein
2205
hypothetical protein

855
hypothetical protein
2206
uncharacterized protein

856
hypothetical protein
2207
putative protein

857
putative aldehyde dehydrogenase
2208
hypothetical protein

858
putative peroxidase
2209
peroxidase (emb|CAA66967.1)

859
UDP-glucose 4-epimerase-like
2210
putative flavonol 3-O-

protein

glucosyltransferase

860
indole-3-acetate beta-
2211
putative flavonol 3-O-

glucosyltransferase like protein

glucosyltransferase

861
putative beta-1,3-glucanase
2212
putative protein

862
disease resistance protein-like
2213
glycerol-3-phosphate

acyltransferase

863
putative respiratory burst oxidase
2214
putative beta-1,3-glucanase

protein B

864
ubiquitin-conjugating enzyme
2215
putative ethylene response element

UBC3

binding protein (EREBP)

865
cytoplasmic aconitate hydratase
2216
putative CONSTANS-like B-box

zinc finger protein

866
NADPH oxidoreductase, putative
2217
putative protein

867
PROTEIN TRANSPORT
2218
unknown protein

PROTEIN SEC61 GAMMA

SUBUNIT-like

868
putative protein
2219
putative trehalose-6-phosphate

phosphatase (AtTPPA)

869
unknown protein
2220
putative protein

870
60S acidic ribosomal protein P2
2221
putative protein

871
No function assigned by TIGR
2222
unknown protein

872
1,4-alpha-glucan branching
2223
unknown prptein

enzyme protein soform SBE2.2

precursor

873
calcium binding protein (CaBP-22)
2224
unknown protein

874
putative phosphoglucomutase
2225
hypothetical protein

875
shaggy-like protein kinase
2226
putative metal-binding

etha (EC 2.7.1.-)

protein

876
pyruvate decarboxylase
2227
putative

(gb|AAB16855.1)

phosphoribosylglycinamide

synthetase

877
hypothetical protein
2228
unknown protein

878
putative protein kinase
2229
putative protein

879
putative protein kinase
2230
unknown protein

880
putative leucine
2231
unknown protein

aminopeptidase

881
probable cytochrome P450
2232
putative beta-galactosidase

882
protein kinase 6-like protein
2233
putative protein kinase

883
arginine methyltransferase
2234
putative protein

(pam1)

884
MYB96 transcription
2235
putative protein

factor-like protein

phosphatase 2C

885
putative protein
2236
putative growth regulator

protein

886
metal ion transporter
2237
putative ABC transporter

887
No function assigned by
2238
chloride channel

TIGR

(emb|CAA70310.1)

888
flax rust resistance protein,
2239
adrenodoxin-like protein

putative

889
fructose-2,6-
2240
NAM (no apical meristem)-

bisphosphatase, putative

like protein

890
exonuclease RRP41
2241
putative transcription factor

MYB41

891
squamosa promoter binding
2242
Myb DNA binding protein-

protein-like 2

like

(emb|CAB56576.1)

892
putative squamosa-
2243
AtMYB84

promoter binding protein

893
O-acetylserine (thiol) lyase,
2244
photosystem II type I

putative

chlorophyll a/b binding

protein

894
snoRNA
2245
putative aspartic proteinase

895
snoRNA
2246
jasmonate inducible

protein, putative

896
ferredoxin-NADP+
2247
putative protein

reductase

897
H+-transporting ATP
2248
No function assigned by

synthase chain 9-like

TIGR

protein

898
photosystem I subunit III
2249
putative phosphatidylserine

precursor, putative

synthase

899
photosystem I subunit VI
2250
putative nicotianamine

precursor

synthase

900
auxin-binding protein 1
2251
lysine and histidine specific

precursor

transporter, putative

901
putative RAS superfamily GTP-
2252
putative protein

binding protein

902
disease resistance protein-like
2253
putative protein

903
protein kinase like protein
2254
putative sugar transporter protein

904
glucuronosyl transferase-like
2255
12S cruciferin seed storage protein

protein

905
putative homeodomain
2256
putative auxin-induced protein,

transcription factor

IAA17/AXR3-1

906
putative flavonol reductase
2257
putative cyclin D

907
putative protein
2258
farnesyl diphosphate synthase

precursor (gb|AAB49290.1)

908
salt-tolerance protein
2259
putative potassium transport

protein (TRH1)

909
40S ribosomal protein S30
2260
putative NPK1-related MAP kinase

910
putative bZIP transcription factor
2261
putative protein

911
putative protein
2262
putative ABC transporter

912
putative cinnamoyl CoA reductase
2263
putative DNA-directed RNA

polymerase subunit

913
unknown protein
2264
putative small nuclear

ribonucleoprotein E

914
putative RNA-binding protein
2265
unknown protein

915
phosphatidylinositol synthase
2266
reticuline oxidase-like protein

(PIS1)

916
unknown protein
2267
putative 1-aminocyclopropane-1-

carboxylate oxidase

917
hydroxyproline-rich glycoprotein
2268
similar to Mlo proteins from H.

homolog

vulgare

918
50S ribosomal protein L15,
2269
long-chain-fatty-acid—CoA ligase-

chloroplast precursor

like protein

919
unknown protein
2270
putative protein

920
putative YME1 ATP-dependant
2271
chromatin remodelling complex

protease

ATPase chain ISWI-like protein

921
unknown protein
2272
hypothetical protein

922
putative ribosomal protein L28
2273
latex-abundant protein, putative

923
unknown protein
2274
N-acetylornithine deacetylase-like

protein, fragment

924
putative protein
2275
putative DNA-binding protein

925
protein ch-42 precursor,
2276
putative anthranilate N-

chloroplast

hydroxycinnamoyl/benzoyltransfer

ase

926
protein serine/threonine kinase,
2277
putative DNA binding protein

putative

927
beta-VPE
2278
cytochrome P450-like protein

928
putative vacuolar sorting receptor
2279
putative DNA-binding protein

929
putative translation initiation factor
2280
putative peptide transporter

IF-2

930
predicted protein of unknown
2281
putative reticuline oxidase-like

function

protein

931
putative protein
2282
thioredoxin, putative

932
hypothetical protein
2283
nodulin-like protein

933
hypothetical protein
2284
UDP-galactose transporter-

like protein

934
phosphate transporter, putative
2285
putative fibrillin

935
No function assigned by
2286
unknown protein

TIGR

936
beta subunit of protein
2287
unknown protein

farnesyl transferase ERA1

937
putative glutamate
2288
unknown protein

decarboxylase

938
putative indole-3-acetate
2289
hypothetical protein

beta-glucosyltransferase

939
putative receptor-like
2290
glyceraldehyde 3-phosphate

protein kinase

dehydrogenase A subunit

(GapA)

940
UDP-galactose 4-
2291
predicted protein of

epimerase-like protein

unknown function

941
putative proliferating cell
2292
putative protein

nuclear antigen, PCNA

942
ubiquitin conjugating
2293
putative protein

enzyme E2 (UBC13)

943
cyclophilin (CYP2)
2294
myb-like protein

944
cystatin
2295
hypothetical protein

(emb|CAA03929.1)

945
putative alcohol
2296
putative U5 small nuclear

dehydrogenase

ribonucleoprotein, an RNA

helicase

946
acidic ribosomal protein p1
2297
unknown protein

947
glutathione transferase
2298
cinnamyl alcohol

AtGST 10

dehydrogenase-like

(emb|CAA10457.1)

protein

948
putative tropinone
2299
hypothetical protein similar

reductase

to extensin-like protein

949
ZIP4, a putative zinc
2300
unknown protein

transporter

950
unknown protein
2301
putative chlorophyll a/b

binding protein

951
putative protein
2302
probable plasma membrane

intrinsic protein 1c

952
putative protein
2303
hexokinase (ATHXK2)

953
putative C2H2-type zinc
2304
calcium-dependent protein

finger protein

kinase

954
putative RING zinc finger
2305
5′-adenylylphosphosulfate

protein

reductase, putative

955
putative microtubule-
2306
Erd1 protein precursor

associated protein

(sp|P42762)

956
unknown protein
2307
putative protein

957
putative protein
2308
putative protein

958
putative protein
2309
unknown protein

phosphatase-2c

959
V-ATPase subunit G (vag2
2310
BCS1 protein-like protein

gene)

960
hypothetical protein
2311
putative protein

961
unknown protein
2312
putative protein

962
unknown protein
2313
putative protein kinase

963
unknown protein
2314
indoleacetic acid (IAA)-inducible

gene (IAA7)

964
myrosinase-associated protein,
2315
ATP-dependent Clp protease

putative

regulatory subunit CLPX

965
hypothetical protein
2316
DNA-binding protein RAV1

966
hypothetical protein
2317
putative protein

967
No function assigned by TIGR
2318
hypothetical protein

968
unknown protein
2319
unknown protein

969
hypothetical protein
2320
unknown protein

970
LAX1/AUX1-like permease
2321
putative protein

971
putative UDP-N-
2322
putative thioredoxin reductase

acetylglucosamine--dolichyl-

phosphate N-

acetylglucosaminephosphotransfer

ase

972
chorismate mutase CM2
2323
unknown protein

973
inner mitochondrial membrane
2324
putative lectin

protein

974
DEF (CLA1) protein
2325
No function assigned by TIGR

975
decoy
2326
beta-fructosidase

976
citrate synthase
2327
chlorophyll a/b-binding protein

CP29

977
myosin
2328
photosystem I subunit PSI-E-like

protein

978
40S ribosomal protein S19
2329
peroxidase ATP8a

979
ripening-related protein-like
2330
putative fructose bisphosphate

aldolase

980
putative signal peptidase I
2331
zinc finger protein ATZF1,

putative

981
methionyl-tRNA synthetase
2332
DegP protease precursor

(AtcpMetRS)

982
ribosomal protein precursor-like
2333
transcription factor-like protein

983
50S ribosomal protein L21
2334
calcium-dependent protein kinase

chloroplast precursor (CL21)

984
putative MYB family transcription
2335
hypothetical protein

factor

985
cyclophilin-like protein
2336
putative protein

986
hypothetical protein
2337
glucose-1-phosphate

adenylyltransferase (APL3)

987
naringenin 3-dioxygenase like
2338
No function assigned by TIGR

protein

988
WD-repeat protein-like protein
2339
putative Eukaryotic initiation factor

4A

989
putative serine carboxypeptidase II
2340
No function assigned by TIGR

990
prenyltransferase, putative
2341
unknown protein

991
putative ligand-gated ion channel
2342
beta tubulin 1, putative

protein

992
clathrin adaptor medium chain
2343
one helix protein (OHP)

protein MU1B, putative

993
No function assigned by TIGR
2344
No function assigned by TIGR

994
putative Tal 1-like non-
2345
zinc finger protein 5, ZFP5

LTR retroelement protein

995
putative 3-isopropylmalate
2346
putative MYB family transcription

dehydrogenase

factor

996
3-isopropylmalate
2347
putative amino acid transporter

dehydratase, small subunit

protein

997
unknown protein
2348
putative potassium

transporter

998
unknown protein
2349
protein kinase (AFC2)

999
unknown protein
2350
putative protein

1000
hypothetical protein
2351
No function assigned by

TIGR

1001
putative protein
2352
putative ubiquitin-

conjugating enzyme E2

1002
No function assigned by
2353
unknown protein

TIGR

1003
putative beta-glucosidase
2354
cytochrome P450

monooxygenase (CYP71B3)

1004
putative pectate lyase A11
2355
putative myrosinase-

binding protein

1005
putative beta-glucosidase
2356
putative vacuolar sorting

receptor

1006
HD-Zip protein
2357
uridine diphosphate glucose

epimerase

1007
putative ubiquitin
2358
shaggy related protein

conjugating enzyme

kinase, ASK-GAMMA

1008
homeobox-leucine zipper
2359
ankyrin repeat protein

protein-like

EMB506

1009
cytochrome P450 like
2360
putative beta-alanine-

protein

pyruvate aminotransferase

1010
putative cysteine proteinase
2361
putative alcohol

inhibitor B (cystatin B)

dehydrogenase

1011
ethylene response sensor
2362
putative receptor-like

(ERS)

protein kinase

1012
putative SWH1 protein
2363
unknown protein

1013
putative glutathione S-
2364
putative methylmalonate

transferase

semi-aldehyde

dehydrogenase

1014
putative protein
2365
hypothetical protein

1015
unknown protein
2366
unknown protein

1016
putative protein
2367
peroxidase ATP13a

phosphatase 2C

1017
dnaJ protein homolog atj3
2368
putative glutathione

peroxidase

1018
ferredoxin
2369
squamosa promoter binding

protein-like 7

1019
hypothetical protein
2370
photosystem II core

complex protein, putative

1020
putative sugar transport
2371
snoRNA

protein, ERD6

1021
putative DnaJ protein
2372
photosystem I subunit X

precursor

1022
putative AP2 domain
2373
MYB transcription factor

transcription factor

(Atmyb2)

1023
putative protein
2374
putative PHD-type zinc finger

protein

1024
putative cyclin-dependent
2375
nuclear RNA binding protein A-

kinase regulatory subunit

like protein

1025
putative tropinone reductase
2376
unknown protein

1026
signal response protein (GAI)
2377
unknown protein

1027
putative steroid sulfotransferase
2378
unknown protein

1028
hypothetical protein
2379
putative amino-cyclopropane-

carboxylic acid oxidase (ACC

oxidase)

1029
nucleic acid binding protein-like
2380
hypothetical protein

1030
putative protein
2381
indole-3-acetate beta-

glucosyltransferase like protein

1031
blue copper binding protein
2382
predicted protein

1032
farnesylated protein (ATFP6)
2383
unknown protein

1033
unknown protein
2384
No function assigned by TIGR

1034
putative PCF2-like DNA binding
2385
putative photosystem I reaction

protein

center subunit IV

1035
teosinte branched1-like protein
2386
putative homeodomain

transcription factor

1036
putative protein
2387
putative purple acid phosphatase

precursor

1037
unknown protein
2388
No function assigned by TIGR

1038
unknown protein
2389
nitrate reductase 1 (NR1)

1039
2-oxoglutarate dehydrogenase, E1
2390
putative casein kinase II beta

component

subunit

1040
unknown protein
2391
pEARLI 1-like protein

1041
unknown protein
2392
putative protein

1042
CCAAT-binding transcription
2393
No function assigned by TIGR

factor subunit A (CBF-A)

1043
hypothetical protein
2394
unknown protein

1044
putative growth regulator protein
2395
putative cell wall-plasma

membrane disconnecting CLCT

protein (AIR1A)

1045
putative presenilin
2396
unknown protein

1046
putative expansin
2397
scarecrow-like 11-like

1047
ribosomal-like protein
2398
putative anthocyanidin synthase

1048
unknown protein
2399
putative AP2 domain transcription

factor

1049
unknown protein
2400
caffeoyl-CoA O-methyltransferase-

like protein

1050
putative protein
2401
unknown protein

1051
putative protein
2402
putative protein kinase

1052
unknown protein
2403
cytochrome P450-like protein

1053
unknown protein
2404
putative MADS-box protein ANR1

1054
unknown protein
2405
putative glutathione S-transferase

1055
unknown protein
2406
hypothetical protein

1056
unknown protein
2407
similar to gibberellin-

regulated proteins

1057
putative protein
2408
unknown protein

1058
putative protein
2409
putative sensory

transduction histidine

kinase

1059
argininosuccinate lyase (AtArgH)
2410
similar to late

embryogenesis abundant

proteins

1060
disease resistance protein homolog
2411
unknown protein

1061
aldehyde dehydrogenase like
2412
putative protein

protein

1062
GBF2, G-box binding factor
2413
putative ATP-dependent

RNA helicase

1063
CDPK-related kinase
2414
putative protein

1064
endo-1,4-beta-glucanase
2415
putative sucrose synthetase

1065
putative serine protease
2416
beta-fructofuranosidase 1

1066
serine/threonine-specific
2417
putative indole-3-acetate

kinase lecRK1 precursor, lectin

beta-glucosyltransferase

receptor-like

1067
putative MAP kinase
2418
hypothetical protein

1068
RNase L inhibitor-like
2419
DNA-directed RNA

protein

polymerase II, third largest subunit

1069
No function assigned by
2420
putative transcription factor

TIGR

1070
AP2 domain transcription
2421
homeobox-leucine zipper

factor

protein ATHB-5 (HD-zip protein

ATHB-5) (sp|P46667)

1071
polygalacturonase
2422
putative ftsH chloroplast

isoenzyme 1 beta subunit,

protease

putative

1072
putative lipid transfer
2423
replication protein A1-like

protein

1073
putative protein kinase
2424
hypothetical protein

1074
putative protein
2425
unknown protein

1075
ATP-dependent RNA
2426
unknown protein

helicase like protein

1076
putative cyclic nucleotide-
2427
putative methionine

regulated ion channel

aminopeptidase

protein

1077
COP1 like protein
2428
unknown protein

1078
putative peroxidase
2429
fatty acid elongase-like

protein (cer2-like)

1079
putative NAK-like ser/thr
2430
unknown protein

protein kinase

1080
putative cytochrome C
2431
putative disease resistance

response protein

1081
cytochrome c
2432
putative protein

1082
putative serine
2433
unknown protein

carboxypeptidase II

1083
acyl-(acyl carrier protein)
2434
putative protein

thioesterase

1084
DNA-binding factor,
2435
putative protein

putative

1085
MAP3K delta-1 protein
2436
unknown protein

kinase

1086
AtMlo-h1-like protein
2437
putative protein

1087
No function assigned by
2438
unknown protein

TIGR

1088
putative expansin
2439
unknown protein

1089
defender against cell death
2440
putative protein

protein, putative

1090
glycolate oxidase-like
2441
No function assigned by TIGR

protein

1091
putative ATP-dependent RNA
2442
MADS-box protein AGL14

helicase

1092
putative protein
2443
No function assigned by TIGR

1093
putative HMG protein
2444
peptidylprolyl isomerase

1094
squalene monooxygenase 2
2445
putative s-adenosylmethionine

(squalene epoxidase 2) (SE 2)

synthetase

(sp|O65403)

1095
eukaryotic peptide chain release
2446
peroxidase

factor subunit 1, putative

1096
auxin-induced protein-like
2447
ferrochelatase-I

1097
putative lipoamide dehydrogenase
2448
putative eukaryotic initiation factor

4, eIF4

1098
putative protein
2449
drought-inducible cysteine

proteinase RD21A precursor-like

protein

1099
unknown protein
2450
unknown protein

1100
putative oligopeptide transporter
2451
unknown protein

1101
putative translation elongation
2452
No function assigned by TIGR

factor ts

1102
putative CCAAT-binding
2453
No function assigned by TIGR

transcription factor subunit

1103
putative ABC transporter
2454
salt-inducible like protein

1104
putative superoxide-generating
2455
glucose-6-phosphate 1-

NADPH oxidase flavocytochrome

dehydrogenase

1105
aspartate kinase-homoserine
2456
3-hydroxy-3-methylglutaryl CoA

dehydrogenase-like protein

reductase (AA 1-592)

1106
putative bHLH transcription factor
2457
hypothetical protein

1107
putative geranylgeranyl transferase
2458
putative protein

type I beta subunit

1108
putative ARP2/3 protein complex
2459
putative putative 60S ribosomal

subunit p41

protein L17

1109
sulphite reductase
2460
putative inorganic pyrophosphatase

1110
putative auxin-regulated protein
2461
putative gamma-

glutamyltransferase

1111
transcription factor scarecrow-like
2462
heat shock transcription factor-

14, putative

like protein

1112
unknown protein
2463
mitochondrial chaperonin hsp60

1113
monooxygenase 2 (MO2)
2464
unknown protein

1114
putative amine oxidase
2465
putative zinc finger protein

identical to T10M13.22

1115
zinc finger protein, putative
2466
putative uridylyl transferase

1116
DNA-binding protein, putative
2467
nodulin-like protein

1117
putative protein
2468
putative B-box zinc finger protein

1118
putative protein
2469
No function assigned by TIGR

1119
Avr9 elicitor response like protein
2470
putative metalloproteinase

1120
putative protein
2471
putative cellular apoptosis

susceptibility protein

1121
hypothetical protein
2472
hypothetical protein

1122
putative nucleotide-sugar
2473
hypothetical protein

dehydratase

1123
UFD1 like protein
2474
scarecrow-like 13 (SCL13)

1124
putative trans-
2475
putative nucleoside

prenyltransferase

triphosphatase

1125
outward rectifying
2476
unknown protein

potassium channel KCO

1126
unknown protein
2477
No function assigned by

TIGR

1127
putative
2478
hypothetical protein

pectinacetylesterase

1128
putative protein
2479
putative phospholipase

1129
No function assigned by
2480
putative snRNP protein

TIGR

1130
unknown protein
2481
putative protein

1131
unknown protein
2482
putative lipase

1132
unknown protein
2483
putative nonsense-mediated

mRNA decay protein

1133
protein phosphatase
2484
No function assigned by

homolog (PPH1)

TIGR

1134
unknown protein
2485
protochlorophyllide

reductase precursor

1135
No function assigned by
2486
No function assigned by

TIGR

TIGR

1136
unknown protein
2487
trehalose-6-phosphate

synthase, putative

1137
unknown protein
2488
unknown protein

1138
unknown protein
2489
germin-like protein

1139
putative protein
2490
plastid protein

1140
unknown protein
2491
putative protein

1141
putative ubiquinol--
2492
hypothetical protein

cytochrome-c reductase

1142
unknown protein
2493
unknown protein

1143
contains similarity to high-
2494
unknown protein

glucose-regulated protein 8

GB:AAF08813 GI:6449083

from [Homo sapiens]

1144
unknown protein
2495
histone deacetylase-like

protein

1145
putative cis-Golgi SNARE
2496
unknown protein

protein

1146
unknown protein
2497
unknown protein

1147
glutamate-1-semialdehyde
2498
putative protein

aminotransferase

1148
No function assigned by
2499
putative protein

TIGR

1149
hypothetical protein
2500
No function assigned by

TIGR

1150
unknown protein
2501
putative zinc transporter

ZIP2-like

1151
unknown protein
2502
unknown protein

1152
unknown protein
2503
putative ribosomal-protein

S6 kinase (ATPK19)

1153
scarecrow-like 3
2504
unknown protein

1154
putative proline-rich protein
2505
unknown protein

1155
cytochrome c oxidoreductase like
2506
60S ribosomal protein L10A

protein

1156
putative
2507
putative protein

carboxymethylenebutenolidase

1157
unknown protein
2508
receptor protein kinase (IRK1),

putative

1158
unknown protein
2509
putative nematode-resistance

protein

1159
unknown protein
2510
tubulin alpha-5 chain-like protein

1160
unknown protein
2511
putative DNA-binding protein

1161
unknown protein
2512
unknown protein

1162
unknown protein
2513
putative RGA1, giberellin repsonse

modulation protein

1163
auxin-induced protein (IAA20)
2514
non phototropic hypocotyl 1-like

1164
50S ribosomal protein L4
2515
RING-H2 finger protein RHA1b

1165
putative DNA topoisomerase III
2516
putative myb-protein

beta

1166
No function assigned by TIGR
2517
hydroperoxide lyase (HPOL) like

protein

1167
isp4 like protein
2518
serine/threonine-protein kinase,

PK7

1168
putative protein kinase
2519
putative vacuolar proton-ATPase

subunit

1169
hypothetical protein
2520
putative polygalacturonase

1170
putative pyrophosphate--fructose-
2521
putative ribosomal protein L8

6-phosphate 1-phosphotransferase

1171
putative protein
2522
putative adenylate kinase

1172
putative protein
2523
germin-like protein (GLP10)

1173
putative protein
2524
putative chlorophyll a/b binding

protein

1174
unknown protein
2525
chloroplast single subunit DNA-

dependent RNA polymerase

1175
unknown protein
2526
putative protein

1176
putative protein
2527
hypothetical protein

1177
putative protein
2528
hypothetical protein

1178
unknown protein
2529
b-keto acyl reductase, putative

1179
unknown protein
2530
cellulose synthase catalytic subunit

1180
putative protein
2531
putative 1-aminocyclopropane-1-

carboxylate oxidase

1181
brassinosteroid insensitive 1 gene
2532
S-linalool synthase, putative

(BRI1)

1182
putative receptor protein kinase
2533
phosphoribosyl-ATP

pyrophosphohydrolase (At-IE)

1183
vacuolar-type H+-translocating
2534
disease resistance RPP5 like

inorganic pyrophosphatase

protein (fragment)

1184
protein kinase-like protein
2535
putative protein

1185
glycyl tRNA synthetase, putative
2536
beta-galactosidase like protein

1186
subtilisin proteinase-like
2537
putative translation

initiation factor eIF-2,

gamma subunit

1187
hypothetical protein
2538
ankyrin like protein

1188
cytochrome P450-like protein
2539
histone H2A-like protein

1189
cytochrome p450 like protein
2540
putative protein

1190
putative protein kinase
2541
salt-tolerance zinc finger

protein

1191
pectinesterase-like protein
2542
unknown protein

1192
putative receptor-like protein
2543
putative protein

kinase

1193
peroxidase ATP17a-like
2544
fructose-bisphosphate

protein

aldolase

1194
No function assigned by
2545
peroxidase

TIGR

(emb|CAA66964.1)

1195
cellulose synthase catalytic
2546
patatin-like protein

subunit-like protein

1196
RAS-related protein, RAB7
2547
salt-inducible protein

homolog

1197
putative aspartate
2548
hypothetical protein

aminotransferase

1198
cyclophilin
2549
xyloglucan endo-

transglycosylase-like

protein

1199
putative SF2/ASF splicing
2550
trihelix DNA-binding

modulator, Srp30

protein (GT2)

1200
putative cytochrome b5
2551
ubiquitin-conjugating

enzyme 16, putative

1201
glutamyl-tRNA reductase,
2552
homeobox protein

putative

1202
putative MADS-box protein
2553
envelope Ca2+-ATPase

1203
ammonium transport
2554
snap25a

protein (AMT1)

1204
No function assigned by
2555
putative annexin

TIGR

1205
putative beta-ketoacyl-CoA
2556
putative protein

synthase

1206
thaumatin-like protein
2557
homeodomain transcription

factor (ATHB-14)

1207
putative methionine
2558
heat shock protein, putative

aminopeptidase

1208
putative protein
2559
peroxidase ATP23a

phosphatase 2C

1209
kinase-like protein
2560
p68 RNA helicase, putative

1210
receptor-associated kinase
2561
potassium transporter,

isolog

putative

1211
mitochondrial ribosomal
2562
putative eukaryotic

protein S14

translation initiation factor 2 alpha

subunit, eIF2

1212
oleosin, 18.5K
2563
hypothetical protein

1213
chalcone isomerase
2564
carnitine racemase like

protein

1214
putative cyclin-dependent
2565
No function assigned by

kinase regulatory subunit

TIGR

1215
putative thaumatin-like
2566
unknown protein

protein

1216
putative two-component
2567
unknown protein

response regulator protein

1217
TATA binding protein-
2568
unknown protein

associated factor, putative

1218
predicted protein of
2569
serine/threonine kinase-like

unknown function

protein

1219
putative AP2 domain transcription
2570
peroxidase (emb|CAA66960.1)

factor

1220
brassinosteroid receptor kinase,
2571
putative protein

putative

1221
TINY-like protein
2572
hypothetical protein

1222
glucose-6-phosphate isomerase
2573
glycine-rich protein 2 (GRP2)

1223
putative protein
2574
unknown protein

1224
putative NAM (no apical
2575
berberine bridge enzyme-like

meristem)-like protein

protein

1225
unknown protein
2576
unknown protein

1226
putative nucleotide-binding protein
2577
putative WD-repeat protein

1227
bZIP transcription factor (POSF21)
2578
serine/threonine kinase-like

protein

1228
ubiquitin activating enzyme-like
2579
serine/threonine kinase-like

protein

protein

1229
telomere repeat-binding protein
2580
Cu2+-transporting ATPase-like

protein

1230
unknown protein
2581
translation initiation factor eIF4E

1231
mevalonate kinase
2582
O-methyltransferase-like protein

1232
putative protein
2583
translation initiation factor eIF3-

like protein

1233
hypothetical protein
2584
No function assigned by TIGR

1234
disease resistance RPP5 like
2585
unknown protein

protein

1235
putative protein
2586
hypothetical protein

1236
putative pectinesterase
2587
unknown protein

1237
Ttg1 protein (emb|CAB45372.1)
2588
unknown protein

1238
FUSCA PROTEIN FUS6
2589
glycine-rich protein like

1239
NHE1 Na+/H+ exchanger
2590
putative disease resistance protein

1240
No function assigned by TIGR
2591
putative Na+/Ca2+ antiporter

1241
Phospholipase like protein
2592
putative hydroxymethylglutaryl-

CoA lyase

1242
unknown protein
2593
putative

phosphoribosylaminoimidazole

carboxylase

1243
unknown protein
2594
SAR DNA-binding protein-like

1244
unknown protein
2595
response regulator, putative

1245
AUX1-like amino acid permease
2596
fibrillin precursor-like protein

1246
unknown protein
2597
beta-ketoacyl-CoA synthase

(FIDDLEHEAD)

1247
putative C2H2-type zinc finger
2598
lectin like protein

protein

1248
putative protein
2599
No function assigned by TIGR

1249
putative protein
2600
acidic endochitinase

(dbj|BAA21861.1)

1250
putative glucosyltransferase
2601
unknown protein

1251
putative lipase
2602
hypothetical protein

1252
putative protein
2603
predicted OR23 protein of

unknown function

1253
putative thioredoxin
2604
putative protein

1254
AIG2-like protein
2605
hypothetical protein

1255
short-chain alcohol dehydrogenase
2606
glycerol-3-phosphate

like protein

dehydrogenase

1256
hypothetical protein
2607
hypothetical protein

1257
putative protein
2608
tat-binding protein, putative

1258
putative protein
2609
putative protein

1259
glutathione peroxidase-
2610
putative trehalose-6-

like protein

phosphate phosphatase

1260
putative protein
2611
hypothetical protein

1261
putative disease resistance
2612
putative flavonol 3-O-

response protein

glucosyltransferase

1262
putative protein
2613
60S ribosomal protein L30

1263
senescence-associated
2614
putative auxin-induced

protein (SAG29)

protein

1264
glycolate oxidase, putative
2615
putative nonspecific lipid-

transfer protein precursor

1265
extensin-like protein
2616
AtRer1A

1266
putative protein
2617
putative aquaporin

(tonoplast intrinsic protein

gamma)

1267
unknown protein
2618
hypothetical protein

1268
putative disease resistance
2619
putative alanine acetyl

protein

transferase

1269
putative receptor-like
2620
putative NADP-dependent

protein kinase

glyceraldehyde-3-

phosphate dehydrogenase

1270
putative receptor-like
2621
putative DNA binding

protein kinase

protein

1271
basic chitinase
2622
putative cystathionine

gamma-synthase

1272
putative pectin
2623
unknown protein

methylesterase

1273
peroxidase ATP N
2624
malate oxidoreductase

(malic enzyme)

1274
class 2 non-symbiotic
2625
unknown protein

hemoglobin

1275
nitrate transporter
2626
cyclic nucleotide-gated

cation channel

1276
Ca2+/H+-exchanging
2627
glyoxalase II, putative

protein-like

1277
putative protein
2628
putative trypsin inhibitor

1278
hydroxynitrile lyase like
2629
unknown protein

protein

1279
putative AP2 domain
2630
unknown protein

transcription factor

1280
pectin methylesterase,
2631
unknown protein

putative

1281
putative protein
2632
nucleosome assembly protein I-like

protein

1282
beta-glucosidase-like
2633
membrane channel like protein

protein

1283
CCAAT box binding factor/
2634
anthocyanin2, putative

transcription factor Hap2a

1284
putative fibrillin
2635
TWIN SISTER OF FT (TSF)

1285
xyloglucan endo-
2636
putative myb-related transcription

transglycosylase

factor

1286
putative 10 kd chaperonin
2637
hypothetical protein

1287
No function assigned by TIGR
2638
putative RING zinc finger protein

1288
serine/threonine protein kinase
2639
amino acid transport protein AAT1

ATPK10

1289
putative lipase
2640
putative protein

1290
choline kinase GmCK2p-like
2641
putative protein

protein

1291
putative sugar transport protein,
2642
xanthine dehydrogenase

ERD6

1292
MYB27 protein-like
2643
xanthine dehydrogenase-like

protein

1293
DNA-binding protein, putative
2644
receptor protein kinase (IRK1),

putative

1294
similar to cold acclimation protein
2645
dehydrin-like protein

WCOR413 [Triticum aestivum]

1295
unknown protein
2646
unknown protein

1296
aquaporin (plasma membrane
2647
aldehyde dehydrogenase homolog,

intrinsic protein 2B)

putative

1297
No function assigned by TIGR
2648
Ran binding protein (AtRanBP1b)

1298
P-Protein-like protein
2649
putative squamosa-promoter

binding protein

1299
No function assigned by TIGR
2650
putative protein

1300
putative cytochrome P450
2651
kinesin like protein

monooxygenase

1301
putative cytochrome P450
2652
putative cellulose synthase

monooxygenase

1302
putative thioredoxin
2653
calmodulin (cam2)

1303
stromal ascorbate peroxidase
2654
fibrillarin-like protein

1304
ethylene responsive element
2655
putative transmembrane protein

binding factor-like protein

G5p

(AtERF6)

1305
auxin transport protein EIR1
2656
putative peroxidase

(gb|AAC39513.1)

1306
putative CONSTANS-like B-box
2657
putative SNF1-related protein

zinc finger protein

kinase

1307
putative protein kinase
2658
glutathione S-transferase, putative

1308
mitochondrial Lon protease
2659
unknown protein

homolog 1 precursor (sp|O64948)

1309
putative protein
2660
hypothetical protein

1310
heme activated protein, putative
2661
putative protein

1311
putative cytochrome P450
2662
phosphatidylinositol-4-phosphate

5-kinase isolog

1312
No function assigned by TIGR
2663
putative tyrosine decarboxylase

1313
putative lipase
2664
unknown protein

1314
putative protein
2665
SGP1 monomeric G-protein

(emb|CAB54517.1)

1315
putative sugar transporter protein
2666
putative serine

carboxypeptidase II

1316
putative sucrose transport protein,
2667
putative L5 ribosomal

SUC2

proteiii

1317
putative protein
2668
putative glucosyltransferase

1318
putative protein
2669
flavonoid 3,5-hydroxylase

like protein

1319
putative endochitinase
2670
putative protein

1320
putative acetone-
2671
putative protein

cyanohydrin lyase

1321
putative protein
2672
putative Fe (II)/ascorbate

oxidase

1322
calmodulin-like protein
2673
putative anthocyanin 5-

aromatic acyltransferase

1323
hypothetical protein
2674
casein kinase I

1324
cysteine proteinase like
2675
putative 2,3-

protein

bisphosphoglycerate-

independent

phosphoglycerate mutase

1325
heat shock protein 17.6-II
2676
putative glutathione S-

transferase TSI-1

1326
heat shock protein 18
2677
ATP-dependent RNA

helicase

1327
Arabidopsis mitochondrion-
2678
putative cytochrome P450

localized small heat shock

protein (AtHSP23.6-mito)

1328
unknown protein
2679
putative WD-40 repeat

protein

1329
putative WRKY-type DNA
2680
No function assigned by

binding protein

TIGR

1330
No function assigned by
2681
No function assigned by

TIGR

TIGR

1331
hypothetical protein
2682
putative protein

1332
putative integral membrane
2683
putative extensin

protein nodulin

1333
putative protein
2684
nodulin-26-like protein

1334
unknown protein
2685
RNA helicase

(emb|CAA09212.1)

1335
3-isopropylmalate
2686
predicted protein of

dehydratase, small subunit

unknown function

1336
unknown protein
2687
putative berberine bridge

enzyme

1337
putative homeodomain
2688
thioredoxin, putative

transcription factor

1338
unknown protein
2689
putative serine

carboxypeptidase I

1339
putative protein
2690
cytochrome P450-like

protein

1340
peroxidase ATP19a
2691
putative pyrophosphate-dependent

phosphofructokinase alpha subunit

1341
putative Na+/H+-
2692
putative flavonol

exchanging protein

glucosyltransferase

1342
putative auxin-regulated
2693
peroxidase ATP20a

protein

(emb|CAA67338.1)

1343
unknown protein
2694
TOPP8 serine/threonine protein

phosphatase type one

1344
unknown protein
2695
auxin regulated protein IAA18,

putative

1345
putative trehalose-6-
2696
putative WRKY-type DNA binding

phosphate synthase

protein

1346
putative lectin
2697
putative glucan synthase

1347
Mlo protein-like
2698
squalene monooxygenase

1348
unknown protein
2699
putative proline-rich protein

1349
ethylene response factor,
2700
G2484-1 protein

putative

1350
unknown protein
2701
heat shock protein 70 like protein

1351
unknown protein
2702
unknown protein

2703
unknown protein

TABLE 2

ABIOTIC STRESS RESPONSIVE

GENE REGULATORY SEQUENCES

SEQ
REGULATORY
SEQ
REGULATORY

ID NO
REGION
ID NO
REGION

1
2704
1353
4046

2
2705
1354
4047

3
2706
1355
4048

4
2707
1356
4049

5
2708
1357
4050

6
2709
1358
4051

7
2710
1359
4052

8
2711
1360
4053

9
2712
1361
4054

10
2713
1362
4055

11
2714
1363
4056

12
2715
1364
4057

13
2716
1365
4058

14
2717
1366
4059

15
2718
1367
4060

16
2719
1368
4061

17
2720
1369
4062

18
2721
1370
4063

19
2722
1371
4064

20
2723
1372
4065

21
2724
1373
4066

22
2725
1374
4067

23
2726
1375
4068

24
2727
1376
4069

25
2728
1377
4070

26
2729
1378
4071

27
2730
1379
4072

28
2731
1380
4073

29
2732
1381
4074

30
2733
1382
4075

31
2734
1383
4076

32
2735
1384
4077

33
2736
1385
4078

34
2737
1386
4079

35
2738
1387
4080

36
2739
1388
4081

37
2740
1389
4082

38
2741
1390
4083

39
2742
1391
4084

40
2743
1392
4085

41
2744
1393
4086

42
2745
1394
4087

43
NONE
1395
4088

44
2746
1396
4089

45
2747
1397
4090

46
2748
1398
4091

47
2749
1399
4092

48
2750
1400
4093

49
2751
1401
4094

50
2752
1402
4095

51
2753
1403
4096

52
2754
1404
4097

53
2755
1405
4098

54
2756
1406
4099

55
2757
1407
4100

56
2758
1408
4101

57
2759
1409
4102

58
2760
1410
4103

59
2761
1411
4104

60
2762
1412
4105

61
2763
1413
4106

62
2764
1414
4107

63
2765
1415
4108

64
2766
1416
4109

65
2767
1417
4110

66
2768
1418
4111

67
2769
1419
4112

68
2770
1420
4113

69
NONE
1421
4114

70
2771
1422
4115

71
2772
1423
4116

72
2773
1424
4117

73
2774
1425
4118

74
2775
1426
4119

75
2776
1427
4120

76
2777
1428
4121

77
2778
1429
4122

78
2779
1430
4123

79
2780
1431
4124

80
2781
1432
NONE

81
2782
1433
4125

82
2783
1434
4126

83
2784
1435
4127

84
2785
1436
4128

85
2786
1437
4129

86
2787
1438
4130

87
2788
1439
4131

88
2789
1440
4132

89
2790
1441
4133

90
2791
1442
4134

91
2792
1443
4135

92
2793
1444
4136

93
2794
1445
4137

94
2795
1446
4138

95
2796
1447
4139

96
2797
1448
4140

97
2798
1449
4141

98
2799
1450
4142

99
2800
1451
4143

100
2801
1452
4144

101
2802
1453
4145

102
2803
1454
4146

103
2804
1455
4147

104
2805
1456
4148

105
2806
1457
4149

106
2807
1458
4150

107
2808
1459
4151

108
2809
1460
4152

109
2810
1461
4153

110
2811
1462
4154

111
2812
1463
4155

112
2813
1464
4156

113
2814
1465
4157

114
2815
1466
4158

115
2816
1467
4159

116
2817
1468
4160

117
2818
1469
4161

118
2819
1470
4162

119
2820
1471
4163

120
2821
1472
4164

121
2822
1473
4165

122
2823
1474
4166

123
2824
1475
4167

124
2825
1476
4168

125
2826
1477
4169

126
2827
1478
4170

127
2828
1479
4171

128
2829
1480
4172

129
2830
1481
4173

130
2831
1482
4174

131
2832
1483
4175

132
2833
1484
4176

133
2834
1485
4177

134
2835
1486
4178

135
2836
1487
4179

136
2837
1488
4180

137
2838
1489
4181

138
2839
1490
4182

139
2840
1491
4183

140
2841
1492
4184

141
2842
1493
4185

142
2843
1494
4186

143
2844
1495
4187

144
NONE
1496
4188

145
2845
1497
4189

146
2846
1498
4190

147
2847
1499
4191

148
2848
1500
4192

149
2849
1501
4193

150
2850
1502
4194

151
2851
1503
4195

152
2852
1504
4196

153
2853
1505
4197

154
2854
1506
4198

155
2855
1507
4199

156
2856
1508
4200

157
2857
1509
4201

158
2858
1510
4202

159
2859
1511
4203

160
2860
1512
4204

161
2861
1513
4205

162
2862
1514
4206

163
2863
1515
4207

164
2864
1516
4208

165
2865
1517
4209

166
2866
1518
4210

167
2867
1519
4211

168
2868
1520
4212

169
2869
1521
4213

170
2870
1522
4214

171
2871
1523
4215

172
2872
1524
4216

173
2873
1525
4217

174
2874
1526
4218

175
2875
1527
4219

176
2876
1528
4220

177
2877
1529
4221

178
2878
1530
4222

179
2879
1531
4223

180
2880
1532
4224

181
2881
1533
4225

182
2882
1534
4226

183
2883
1535
4227

184
2884
1536
4228

185
2885
1537
4229

186
2886
1538
4230

187
2887
1539
4231

188
2888
1540
4232

189
2889
1541
4233

190
2890
1542
4234

191
2891
1543
4235

192
2892
1544
4236

193
2893
1545
4237

194
2894
1546
4238

195
2895
1547
4239

196
2896
1548
4240

197
2897
1549
4241

198
2898
1550
4242

199
2899
1551
4243

200
2900
1552
4244

201
2901
1553
4245

202
2902
1554
4246

203
2903
1555
4247

204
2904
1556
4248

205
2905
1557
4249

206
2906
1558
NONE

207
2907
1559
4250

208
2908
1560
4251

209
2909
1561
4252

210
2910
1562
4253

211
2911
1563
4254

212
2912
1564
4255

213
2913
1565
4256

214
2914
1566
4257

215
2915
1567
4258

216
2916
1568
4259

217
2917
1569
4260

218
2918
1570
4261

219
2919
1571
4262

220
2920
1572
4263

221
2921
1573
4264

222
2922
1574
4265

223
2923
1575
4266

224
2924
1576
4267

225
2925
1577
4268

226
2926
1578
4269

227
2927
1579
4270

228
2928
1580
4271

229
2929
1581
4272

230
2930
1582
4273

231
2931
1583
4274

232
2932
1584
4275

233
2933
1585
4276

234
2934
1586
4277

235
2935
1587
4278

236
2936
1588
4279

237
2937
1589
4280

238
2938
1590
4281

239
2939
1591
4282

240
2940
1592
4283

241
2941
1593
4284

242
2942
1594
4285

243
2943
1595
4286

244
2944
1596
4287

245
2945
1597
4288

246
2946
1598
4289

247
2947
1599
4290

248
2948
1600
4291

249
2949
1601
4292

250
2950
1602
4293

251
2951
1603
4294

252
2952
1604
4295

253
2953
1605
4296

254
2954
1606
4297

255
2955
1607
4298

256
2956
1608
4299

257
2957
1609
4300

258
2958
1610
4301

259
2959
1611
4302

260
2960
1612
4303

261
2961
1613
4304

262
2962
1614
4305

263
2963
1615
4306

264
2964
1616
4307

265
2965
1617
4308

266
2966
1618
4309

267
2967
1619
4310

268
2968
1620
4311

269
2969
1621
4312

270
2970
1622
4313

271
2971
1623
4314

272
2972
1624
4315

273
2973
1625
4316

274
2974
1626
4317

275
2975
1627
4318

276
2976
1628
4319

277
2977
1629
4320

278
2978
1630
4321

279
2979
1631
4322

280
2980
1632
4323

281
2981
1633
4324

282
2982
1634
4325

283
2983
1635
4326

284
2984
1636
4327

285
2985
1637
4328

286
2986
1638
4329

287
2987
1639
4330

288
2988
1640
4331

289
2989
1641
4332

290
2990
1642
4333

291
2991
1643
4334

292
2992
1644
4335

293
2993
1645
4336

294
2994
1646
4337

295
2995
1647
4338

296
2996
1648
4339

297
2997
1649
4340

298
2998
1650
4341

299
2999
1651
4342

300
3000
1652
4343

301
3001
1653
4344

302
3002
1654
4345

303
3003
1655
4346

304
NONE
1656
4347

305
3004
1657
4348

306
3005
1658
4349

307
3006
1659
4350

308
3007
1660
4351

309
3008
1661
4352

310
3009
1662
4353

311
3010
1663
NONE

312
3011
1664
4354

313
3012
1665
4355

314
3013
1666
4356

315
3014
1667
4357

316
3015
1668
4358

317
3016
1669
4359

318
3017
1670
4360

319
3018
1671
4361

320
3019
1672
4362

321
3020
1673
4363

322
3021
1674
4364

323
3022
1675
4365

324
3023
1676
4366

325
3024
1677
4367

326
3025
1678
4368

327
3026
1679
4369

328
3027
1680
4370

329
3028
1681
4371

330
3029
1682
4372

331
3030
1683
4373

332
3031
1684
4374

333
3032
1685
4375

334
3033
1686
4376

335
3034
1687
4377

336
3035
1688
4378

337
3036
1689
4379

338
3037
1690
4380

339
3038
1691
4381

340
3039
1692
4382

341
3040
1693
4383

342
3041
1694
4384

343
3042
1695
4385

344
3043
1696
4386

345
3044
1697
4387

346
3045
1698
4388

347
3046
1699
4389

348
3047
1700
4390

349
3048
1701
4391

350
3049
1702
4392

351
3050
1703
4393

352
3051
1704
4394

353
3052
1705
4395

354
3053
1706
4396

355
3054
1707
4397

356
3055
1708
4398

357
3056
1709
4399

358
3057
1710
4400

359
3058
1711
4401

360
3059
1712
NONE

361
3060
1713
4402

362
3061
1714
4403

363
3062
1715
4404

364
3063
1716
4405

365
3064
1717
4406

366
3065
1718
4407

367
3066
1719
4408

368
3067
1720
4409

369
3068
1721
4410

370
3069
1722
4411

371
3070
1723
4412

372
3071
1724
4413

373
3072
1725
4414

374
3073
1726
4415

375
3074
1727
4416

376
3075
1728
4417

377
3076
1729
4418

378
3077
1730
4419

379
3078
1731
4420

380
3079
1732
4421

381
3080
1733
4422

382
3081
1734
4423

383
3082
1735
4424

384
3083
1736
4425

385
3084
1737
4426

386
3085
1738
4427

387
3086
1739
4428

388
3087
1740
4429

389
3088
1741
4430

390
3089
1742
4431

391
3090
1743
4432

392
3091
1744
4433

393
3092
1745
4434

394
3093
1746
4435

395
3094
1747
4436

396
3095
1748
4437

397
3096
1749
4438

398
3097
1750
4439

399
3098
1751
4440

400
3099
1752
4441

401
3100
1753
4442

402
3101
1754
4443

403
3102
1755
4444

404
3103
1756
4445

405
3104
1757
4446

406
3105
1758
4447

407
3106
1759
4448

408
3107
1760
4449

409
3108
1761
4450

410
3109
1762
4451

411
3110
1763
4452

412
3111
1764
4453

413
3112
1765
4454

414
3113
1766
4455

415
3114
1767
4456

416
3115
1768
4457

417
3116
1769
4458

418
3117
1770
4459

419
3118
1771
4460

420
3119
1772
4461

421
3120
1773
4462

422
3121
1774
4463

423
3122
1775
4464

424
3123
1776
4465

425
3124
1777
4466

426
3125
1778
4467

427
3126
1779
4468

428
3127
1780
4469

429
3128
1781
4470

430
3129
1782
4471

431
3130
1783
4472

432
3131
1784
NONE

433
3132
1785
4473

434
3133
1786
4474

435
3134
1787
4475

436
3135
1788
4476

437
3136
1789
4477

438
3137
1790
4478

439
3138
1791
4479

440
3139
1792
4480

441
3140
1793
4481

442
3141
1794
4482

443
3142
1795
4483

444
3143
1796
4484

445
3144
1797
4485

446
3145
1798
4486

447
3146
1799
4487

448
3147
1800
4488

449
3148
1801
4489

450
3149
1802
4490

451
3150
1803
NONE

452
3151
1804
4491

453
3152
1805
4492

454
3153
1806
4493

455
3154
1807
4494

456
3155
1808
4495

457
3156
1809
4496

458
3157
1810
4497

459
3158
1811
4498

460
3159
1812
4499

461
3160
1813
4500

462
3161
1814
4501

463
3162
1815
4502

464
3163
1816
4503

465
3164
1817
4504

466
3165
1818
4505

467
3166
1819
4506

468
3167
1820
4507

469
3168
1821
4508

470
3169
1822
4509

471
3170
1823
4510

472
3171
1824
4511

473
3172
1825
4512

474
3173
1826
4513

475
3174
1827
4514

476
3175
1828
4515

477
3176
1829
4516

478
3177
1830
4517

479
3178
1831
4518

480
3179
1832
4519

481
3180
1833
4520

482
3181
1834
4521

483
3182
1835
4522

484
3183
1836
4523

485
3184
1837
4524

486
3185
1838
4525

487
3186
1839
4526

488
3187
1840
4527

489
3188
1841
4528

490
3189
1842
4529

491
3190
1843
4530

492
3191
1844
4531

493
3192
1845
4532

494
3193
1846
4533

495
3194
1847
4534

496
3195
1848
4535

497
3196
1849
4536

498
3197
1850
4537

499
3198
1851
4538

500
3199
1852
4539

501
3200
1853
4540

502
3201
1854
4541

503
3202
1855
4542

504
3203
1856
4543

505
3204
1857
4544

506
3205
1858
4545

507
3206
1859
4546

508
3207
1860
4547

509
3208
1861
4548

510
3209
1862
4549

511
3210
1863
4550

512
3211
1864
4551

513
3212
1865
4552

514
3213
1866
4553

515
3214
1867
4554

516
3215
1868
4555

517
3216
1869
4556

518
3217
1870
4557

519
3218
1871
4558

520
3219
1872
4559

521
3220
1873
4560

522
3221
1874
4561

523
3222
1875
4562

524
3223
1876
4563

525
3224
1877
4564

526
3225
1878
4565

527
3226
1879
4566

528
3227
1880
4567

529
3228
1881
4568

530
3229
1882
4569

531
3230
1883
4570

532
3231
1884
4571

533
3232
1885
4572

534
3233
1886
4573

535
3234
1887
4574

536
3235
1888
4575

537
3236
1889
4576

538
3237
1890
4577

539
3238
1891
4578

540
3239
1892
4579

541
3240
1893
4580

542
3241
1894
4581

543
3242
1895
4582

544
3243
1896
4583

545
3244
1897
NONE

546
3245
1898
4584

547
3246
1899
4585

548
3247
1900
4586

549
3248
1901
4587

550
3249
1902
4588

551
3250
1903
4589

552
3251
1904
4590

553
3252
1905
4591

554
3253
1906
4592

555
3254
1907
NONE

556
3255
1908
4593

557
3256
1909
4594

558
3257
1910
4595

559
3258
1911
4596

560
3259
1912
4597

561
3260
1913
4598

562
3261
1914
4599

563
3262
1915
4600

564
3263
1916
4601

565
3264
1917
4602

566
3265
1918
4603

567
3266
1919
4604

568
3267
1920
4605

569
3268
1921
4606

570
3269
1922
4607

571
3270
1923
4608

572
3271
1924
4609

573
3272
1925
4610

574
3273
1926
4611

575
3274
1927
4612

576
3275
1928
4613

577
3276
1929
4614

578
3277
1930
4615

579
3278
1931
4616

580
3279
1932
4617

581
3280
1933
4618

582
3281
1934
4619

583
3282
1935
4620

584
3283
1936
4621

585
3284
1937
4622

586
3285
1938
4623

587
3286
1939
4624

588
3287
1940
4625

589
3288
1941
4626

590
3289
1942
4627

591
3290
1943
4628

592
3291
1944
4629

593
3292
1945
4630

594
3293
1946
4631

595
3294
1947
4632

596
3295
1948
4633

597
3296
1949
4634

598
3297
1950
4635

599
3298
1951
4636

600
3299
1952
4637

601
3300
1953
4638

602
3301
1954
4639

603
3302
1955
4640

604
3303
1956
4641

605
3304
1957
4642

606
3305
1958
4643

607
3306
1959
4644

608
3307
1960
4645

609
3308
1961
4646

610
3309
1962
4647

611
3310
1963
4648

612
3311
1964
4649

613
3312
1965
4650

614
3313
1966
4651

615
3314
1967
4652

616
3315
1968
4653

617
3316
1969
4654

618
3317
1970
4655

619
3318
1971
4656

620
3319
1972
4657

621
3320
1973
4658

622
3321
1974
4659

623
3322
1975
4660

624
3323
1976
4661

625
3324
1977
4662

626
3325
1978
4663

627
3326
1979
4664

628
3327
1980
4665

629
3328
1981
4666

630
3329
1982
4667

631
3330
1983
4668

632
3331
1984
4669

633
3332
1985
4670

634
3333
1986
4671

635
3334
1987
4672

636
3335
1988
4673

637
3336
1989
4674

638
3337
1990
4675

639
3338
1991
4676

640
3339
1992
4677

641
3340
1993
4678

642
3341
1994
4679

643
3342
1995
4680

644
3343
1996
4681

645
3344
1997
4682

646
3345
1998
4683

647
3346
1999
4684

648
3347
2000
4685

649
3348
2001
4686

650
3349
2002
4687

651
3350
2003
4688

652
3351
2004
4689

653
3352
2005
4690

654
3353
2006
4691

655
3354
2007
4692

656
3355
2008
4693

657
3356
2009
4694

658
3357
2010
4695

659
3358
2011
4696

660
3359
2012
4697

661
3360
2013
4698

662
3361
2014
4699

663
3362
2015
4700

664
3363
2016
4701

665
3364
2017
4702

666
3365
2018
4703

667
3366
2019
4704

668
3367
2020
4705

669
3368
2021
4706

670
3369
2022
4707

671
3370
2023
4708

672
3371
2024
4709

673
3372
2025
4710

674
3373
2026
4711

675
3374
2027
4712

676
3375
2028
4713

677
3376
2029
4714

678
3377
2030
NONE

679
3378
2031
4715

680
3379
2032
4716

681
3380
2033
4717

682
3381
2034
4718

683
3382
2035
4719

684
3383
2036
4720

685
3384
2037
4721

686
3385
2038
4722

687
3386
2039
4723

688
3387
2040
4724

689
3388
2041
4725

690
3389
2042
4726

691
3390
2043
4727

692
3391
2044
4728

693
3392
2045
4729

694
3393
2046
4730

695
3394
2047
4731

696
3395
2048
4732

697
3396
2049
4733

698
3397
2050
4734

699
3398
2051
4735

700
3399
2052
4736

701
3400
2053
4737

702
3401
2054
4738

703
3402
2055
4739

704
3403
2056
4740

705
3404
2057
4741

706
3405
2058
4742

707
3406
2059
4743

708
3407
2060
4744

709
3408
2061
4745

710
3409
2062
4746

711
3410
2063
4747

712
3411
2064
4748

713
3412
2065
4749

714
3413
2066
4750

715
3414
2067
4751

716
3415
2068
4752

717
3416
2069
4753

718
3417
2070
4754

719
3418
2071
4755

720
3419
2072
4756

721
3420
2073
4757

722
3421
2074
4758

723
3422
2075
4759

724
3423
2076
4760

725
3424
2077
4761

726
3425
2078
4762

727
3426
2079
4763

728
3427
2080
4764

729
3428
2081
4765

730
3429
2082
4766

731
3430
2083
4767

732
3431
2084
4768

733
3432
2085
4769

734
3433
2086
4770

735
3434
2087
4771

736
3435
2088
4772

737
3436
2089
4773

738
3437
2090
4774

739
3438
2091
4775

740
3439
2092
4776

741
3440
2093
4777

742
3441
2094
4778

743
3442
2095
4779

744
3443
2096
4780

745
3444
2097
4781

746
3445
2098
4782

747
3446
2099
4783

748
3447
2100
4784

749
3448
2101
4785

750
3449
2102
4786

751
3450
2103
4787

752
3451
2104
4788

753
3452
2105
4789

754
3453
2106
4790

755
3454
2107
4791

756
3455
2108
4792

757
3456
2109
4793

758
3457
2110
4794

759
3458
2111
4795

760
3459
2112
4796

761
3460
2113
4797

762
3461
2114
4798

763
3462
2115
4799

764
3463
2116
4800

765
3464
2117
4801

766
3465
2118
4802

767
3466
2119
4803

768
3467
2120
4804

769
3468
2121
4805

770
3469
2122
4806

771
3470
2123
4807

772
3471
2124
4808

773
3472
2125
4809

774
3473
2126
4810

775
3474
2127
4811

776
3475
2128
4812

777
3476
2129
4813

778
3477
2130
4814

779
3478
2131
4815

780
3479
2132
4816

781
3480
2133
4817

782
3481
2134
4818

783
3482
2135
4819

784
3483
2136
4820

785
3484
2137
4821

786
3485
2138
4822

787
3486
2139
4823

788
3487
2140
4824

789
3488
2141
4825

790
3489
2142
4826

791
3490
2143
4827

792
3491
2144
4828

793
3492
2145
4829

794
3493
2146
4830

795
3494
2147
4831

796
3495
2148
4832

797
3496
2149
4833

798
3497
2150
4834

799
3498
2151
NONE

800
3499
2152
4835

801
3500
2153
4836

802
3501
2154
4837

803
3502
2155
4838

804
3503
2156
4839

805
3504
2157
4840

806
3505
2158
4841

807
3506
2159
4842

808
3507
2160
4843

809
3508
2161
4844

810
3509
2162
4845

811
3510
2163
4846

812
3511
2164
4847

813
3512
2165
4848

814
3513
2166
4849

815
3514
2167
4850

816
3515
2168
4851

817
3516
2169
4852

818
3517
2170
4853

819
3518
2171
4854

820
3519
2172
4855

821
3520
2173
4856

822
3521
2174
4857

823
3522
2175
4858

824
3523
2176
4859

825
3524
2177
4860

826
3525
2178
4861

827
3526
2179
4862

828
3527
2180
4863

829
3528
2181
4864

830
3529
2182
4865

831
3530
2183
4866

832
3531
2184
4867

833
3532
2185
4868

834
3533
2186
4869

835
3534
2187
4870

836
3535
2188
4871

837
3536
2189
4872

838
3537
2190
4873

839
3538
2191
4874

840
3539
2192
4875

841
3540
2193
4876

842
3541
2194
4877

843
3542
2195
4878

844
3543
2196
4879

845
3544
2197
4880

846
3545
2198
4881

847
3546
2199
4882

848
3547
2200
4883

849
3548
2201
4884

850
3549
2202
4885

851
3550
2203
4886

852
3551
2204
4887

853
3552
2205
4888

854
3553
2206
4889

855
3554
2207
4890

856
3555
2208
4891

857
3556
2209
4892

858
3557
2210
4893

859
3558
2211
4894

860
3559
2212
4895

861
3560
2213
4896

862
3561
2214
4897

863
3562
2215
4898

864
3563
2216
4899

865
3564
2217
4900

866
3565
2218
4901

867
3566
2219
4902

868
3567
2220
4903

869
3568
2221
4904

870
3569
2222
4905

871
3570
2223
4906

872
3571
2224
4907

873
3572
2225
4908

874
3573
2226
4909

875
3574
2227
4910

876
3575
2228
4911

877
3576
2229
4912

878
3577
2230
4913

879
3578
2231
4914

880
3579
2232
4915

881
3580
2233
4916

882
3581
2234
4917

883
3582
2235
4918

884
3583
2236
4919

885
3584
2237
4920

886
3585
2238
4921

887
NONE
2239
4922

888
3586
2240
4923

889
3587
2241
4924

890
3588
2242
4925

891
3589
2243
4926

892
3590
2244
4927

893
3591
2245
4928

894
NONE
2246
4929

895
NONE
2247
4930

896
3592
2248
NONE

897
3593
2249
4931

898
3594
2250
4932

899
3595
2251
4933

900
3596
2252
4934

901
3597
2253
4935

902
3598
2254
4936

903
3599
2255
4937

904
3600
2256
4938

905
3601
2257
4939

906
3602
2258
4940

907
3603
2259
4941

908
3604
2260
4942

909
3605
2261
4943

910
3606
2262
4944

911
3607
2263
4945

912
3608
2264
4946

913
3609
2265
4947

914
3610
2266
4948

915
3611
2267
4949

916
3612
2268
4950

917
3613
2269
4951

918
3614
2270
4952

919
3615
2271
4953

920
3616
2272
4954

921
3617
2273
4955

922
3618
2274
4956

923
3619
2275
4957

924
3620
2276
4958

925
3621
2277
4959

926
3622
2278
4960

927
3623
2279
4961

928
3624
2280
4962

929
3625
2281
4963

930
3626
2282
4964

931
3627
2283
4965

932
3628
2284
4966

933
3629
2285
4967

934
3630
2286
4968

935
NONE
2287
4969

936
3631
2288
4970

937
3632
2289
4971

938
3633
2290
4972

939
3634
2291
4973

940
3635
2292
4974

941
3636
2293
4975

942
3637
2294
4976

943
3638
2295
4977

944
3639
2296
4978

945
3640
2297
4979

946
3641
2298
4980

947
3642
2299
4981

948
3643
2300
4982

949
3644
2301
4983

950
3645
2302
4984

951
3646
2303
4985

952
3647
2304
4986

953
3648
2305
4987

954
3649
2306
4988

955
3650
2307
4989

956
3651
2308
4990

957
3652
2309
4991

958
3653
2310
4992

959
3654
2311
4993

960
3655
2312
4994

961
3656
2313
4995

962
3657
2314
4996

963
3658
2315
4997

964
3659
2316
4998

965
3660
2317
4999

966
3661
2318
5000

967
3662
2319
5001

968
3663
2320
5002

969
3664
2321
5003

970
3665
2322
5004

971
3666
2323
5005

972
3667
2324
5006

973
3668
2325
5007

974
3669
2326
5008

975
3670
2327
5009

976
3671
2328
5010

977
3672
2329
5011

978
3673
2330
5012

979
3674
2331
5013

980
3675
2332
5014

981
3676
2333
5015

982
3677
2334
5016

983
3678
2335
5017

984
3679
2336
5018

985
3680
2337
5019

986
3681
2338
5020

987
3682
2339
5021

988
3683
2340
NONE

989
3684
2341
5022

990
3685
2342
5023

991
3686
2343
5024

992
3687
2344
5025

993
3688
2345
5026

994
3689
2346
5027

995
3690
2347
5028

996
3691
2348
5029

997
3692
2349
5030

998
3693
2350
5031

999
3694
2351
5032

1000
3695
2352
5033

1001
3696
2353
5034

1002
3697
2354
5035

1003
3698
2355
5036

1004
3699
2356
5037

1005
3700
2357
5038

1006
3701
2358
5039

1007
3702
2359
5040

1008
3703
2360
5041

1009
3704
2361
5042

1010
3705
2362
5043

1011
3706
2363
5044

1012
3707
2364
5045

1013
3708
2365
5046

1014
3709
2366
5047

1015
3710
2367
5048

1016
3711
2368
5049

1017
3712
2369
5050

1018
3713
2370
5051

1019
3714
2371
NONE

1020
3715
2372
5052

1021
3716
2373
5053

1022
3717
2374
5054

1023
3718
2375
5055

1024
3719
2376
5056

1025
3720
2377
5057

1026
3721
2378
5058

1027
3722
2379
5059

1028
3723
2380
5060

1029
3724
2381
5061

1030
3725
2382
5062

1031
3726
2383
5063

1032
3727
2384
5064

1033
3728
2385
5065

1034
3729
2386
5066

1035
3730
2387
5067

1036
3731
2388
5068

1037
3732
2389
5069

1038
3733
2390
5070

1039
3734
2391
5071

1040
3735
2392
5072

1041
3736
2393
5073

1042
3737
2394
5074

1043
3738
2395
5075

1044
3739
2396
5076

1045
3740
2397
5077

1046
3741
2398
5078

1047
3742
2399
5079

1048
3743
2400
5080

1049
3744
2401
5081

1050
3745
2402
5082

1051
3746
2403
5083

1052
3747
2404
5084

1053
3748
2405
5085

1054
3749
2406
5086

1055
3750
2407
5087

1056
3751
2408
5088

1057
3752
2409
5089

1058
3753
2410
5090

1059
3754
2411
5091

1060
3755
2412
5092

1061
3756
2413
5093

1062
3757
2414
5094

1063
3758
2415
5095

1064
3759
2416
5096

1065
3760
2417
5097

1066
3761
2418
5098

1067
3762
2419
5099

1068
3763
2420
5100

1069
3764
2421
5101

1070
3765
2422
5102

1071
3766
2423
5103

1072
3767
2424
5104

1073
3768
2425
5105

1074
3769
2426
5106

1075
3770
2427
5107

1076
3771
2428
5108

1077
3772
2429
5109

1078
3773
2430
5110

1079
3774
2431
5111

1080
3775
2432
5112

1081
3776
2433
5113

1082
3777
2434
5114

1083
3778
2435
5115

1084
3779
2436
5116

1085
3780
2437
5117

1086
3781
2438
5118

1087
NONE
2439
5119

1088
3782
2440
5120

1089
3783
2441
5121

1090
3784
2442
5122

1091
3785
2443
NONE

1092
3786
2444
5123

1093
3787
2445
5124

1094
3788
2446
5125

1095
3789
2447
5126

1096
3790
2448
5127

1097
3791
2449
5128

1098
3792
2450
5129

1099
3793
2451
5130

1100
3794
2452
5131

1101
3795
2453
5132

1102
3796
2454
5133

1103
3797
2455
5134

1104
3798
2456
5135

1105
3799
2457
5136

1106
3800
2458
5137

1107
3801
2459
5138

1108
3802
2460
5139

1109
3803
2461
5140

1110
3804
2462
5141

1111
3805
2463
5142

1112
3806
2464
5143

1113
3807
2465
5144

1114
3808
2466
5145

1115
3809
2467
5146

1116
3810
2468
5147

1117
3811
2469
NONE

1118
3812
2470
5148

1119
3813
2471
5149

1120
3814
2472
5150

1121
3815
2473
5151

1122
3816
2474
5152

1123
3817
2475
5153

1124
3818
2476
5154

1125
3819
2477
5155

1126
3820
2478
5156

1127
3821
2479
5157

1128
3822
2480
5158

1129
3823
2481
5159

1130
3824
2482
5160

1131
3825
2483
5161

1132
3826
2484
5162

1133
3827
2485
5163

1134
3828
2486
5164

1135
3829
2487
5165

1136
3830
2488
5166

1137
3831
2489
5167

1138
3832
2490
5168

1139
3833
2491
5169

1140
3834
2492
5170

1141
3835
2493
5171

1142
3836
2494
5172

1143
3837
2495
5173

1144
3838
2496
5174

1145
3839
2497
5175

1146
3840
2498
5176

1147
3841
2499
5177

1148
3842
2500
5178

1149
3843
2501
5179

1150
3844
2502
5180

1151
3845
2503
5181

1152
3846
2504
5182

1153
3847
2505
5183

1154
3848
2506
5184

1155
3849
2507
5185

1156
3850
2508
5186

1157
3851
2509
5187

1158
3852
2510
5188

1159
3853
2511
5189

1160
3854
2512
5190

1161
3855
2513
5191

1162
3856
2514
5192

1163
3857
2515
5193

1164
3858
2516
5194

1165
3859
2517
5195

1166
3860
2518
5196

1167
3861
2519
5197

1168
3862
2520
5198

1169
3863
2521
5199

1170
3864
2522
5200

1171
3865
2523
5201

1172
3866
2524
5202

1173
3867
2525
5203

1174
3868
2526
5204

1175
3869
2527
5205

1176
3870
2528
5206

1177
3871
2529
5207

1178
3872
2530
5208

1179
3873
2531
5209

1180
3874
2532
5210

1181
3875
2533
5211

1182
3876
2534
5212

1183
3877
2535
5213

1184
3878
2536
5214

1185
3879
2537
5215

1186
3880
2538
5216

1187
3881
2539
5217

1188
3882
2540
5218

1189
3883
2541
5219

1190
3884
2542
5220

1191
3885
2543
5221

1192
3886
2544
5222

1193
3887
2545
5223

1194
3888
2546
5224

1195
3889
2547
5225

1196
3890
2548
5226

1197
3891
2549
5227

1198
3892
2550
5228

1199
3893
2551
5229

1200
3894
2552
5230

1201
3895
2553
5231

1202
3896
2554
5232

1203
3897
2555
5233

1204
3898
2556
5234

1205
3899
2557
5235

1206
3900
2558
5236

1207
3901
2559
5237

1208
3902
2560
5238

1209
3903
2561
5239

1210
3904
2562
5240

1211
3905
2563
5241

1212
3906
2564
5242

1213
3907
2565
5243

1214
3908
2566
5244

1215
3909
2567
5245

1216
3910
2568
5246

1217
3911
2569
5247

1218
3912
2570
5248

1219
3913
2571
5249

1220
3914
2572
5250

1221
3915
2573
5251

1222
3916
2574
5252

1223
3917
2575
5253

1224
3918
2576
5254

1225
3919
2577
5255

1226
3920
2578
5256

1227
3921
2579
5257

1228
3922
2580
5258

1229
3923
2581
5259

1230
3924
2582
5260

1231
3925
2583
5261

1232
3926
2584
5262

1233
3927
2585
5263

1234
3928
2586
5264

1235
3929
2587
5265

1236
3930
2588
5266

1237
3931
2589
5267

1238
3932
2590
5268

1239
3933
2591
5269

1240
3934
2592
5270

1241
3935
2593
5271

1242
3936
2594
5272

1243
3937
2595
5273

1244
3938
2596
5274

1245
3939
2597
5275

1246
3940
2598
5276

1247
3941
2599
NONE

1248
3942
2600
5277

1249
3943
2601
5278

1250
3944
2602
5279

1251
3945
2603
5280

1252
3946
2604
5281

1253
3947
2605
5282

1254
3948
2606
5283

1255
3949
2607
5284

1256
3950
2608
5285

1257
3951
2609
5286

1258
3952
2610
5287

1259
3953
2611
5288

1260
3954
2612
5289

1261
3955
2613
5290

1262
3956
2614
5291

1263
3957
2615
5292

1264
3958
2616
5293

1265
3959
2617
5294

1266
3960
2618
5295

1267
3961
2619
5296

1268
3962
2620
5297

1269
3963
2621
5298

1270
3964
2622
5299

1271
3965
2623
5300

1272
3966
2624
5301

1273
3967
2625
5302

1274
3968
2626
5303

1275
3969
2627
5304

1276
3970
2628
5305

1277
3971
2629
5306

1278
3972
2630
5307

1279
3973
2631
5308

1280
3974
2632
5309

1281
3975
2633
5310

1282
3976
2634
5311

1283
3977
2635
5312

1284
3978
2636
5313

1285
3979
2637
5314

1286
3980
2638
5315

1287
3981
2639
5316

1288
3982
2640
5317

1289
3983
2641
5318

1290
3984
2642
5319

1291
3985
2643
5320

1292
3986
2644
5321

1293
3987
2645
5322

1294
3988
2646
5323

1295
3989
2647
5324

1296
3990
2648
5325

1297
3991
2649
5326

1298
3992
2650
5327

1299
3993
2651
5328

1300
3994
2652
5329

1301
3995
2653
5330

1302
3996
2654
5331

1303
3997
2655
5332

1304
3998
2656
5333

1305
3999
2657
5334

1306
4000
2658
5335

1307
4001
2659
5336

1308
4002
2660
5337

1309
4003
2661
5338

1310
4004
2662
5339

1311
4005
2663
5340

1312
4006
2664
5341

1313
4007
2665
5342

1314
4008
2666
5343

1315
4009
2667
5344

1316
4010
2668
5345

1317
4011
2669
5346

1318
4012
2670
5347

1319
4013
2671
5348

1320
4014
2672
5349

1321
4015
2673
5350

1322
4016
2674
5351

1323
4017
2675
5352

1324
4018
2676
5353

1325
4019
2677
5354

1326
4020
2678
5355

1327
4021
2679
5356

1328
4022
2680
5357

1329
4023
2681
NONE

1330
NONE
2682
5358

1331
4024
2683
5359

1332
4025
2684
5360

1333
4026
2685
5361

1334
4027
2686
5362

1335
4028
2687
5363

1336
4029
2688
5364

1337
4030
2689
5365

1338
4031
2690
5366

1339
4032
2691
5367

1340
4033
2692
5368

1341
4034
2693
5369

1342
4035
2694
5370

1343
4036
2695
5371

1344
4037
2696
5372

1345
4038
2697
5373

1346
4039
2698
5374

1347
4040
2699
5375

1348
4041
2700
5376

1349
4042
2701
5377

1350
4043
2702
5378

1351
4044
2703
5379

1352
4045

TABLE 3

COLD RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX

ID NO:
ID NO:

1
11991_G_AT

2
11992_AT

3
11997_AT

4
11998_AT

5
12001_AT

6
12006_S_AT

7
12007_AT

8
12009_AT

9
12018_AT

10
12022_AT

11
12026_AT

12
12031_AT

13
12047_AT

14
12051_AT

15
12052_AT

16
12053_AT

17
12060_AT

18
12072_AT

19
12074_AT

20
12102_AT

21
12112_AT

22
12117_AT

23
12125_AT

24
12130_AT

25
12143_AT

26
12145_S_AT

27
12149_AT

28
12156_AT

29
12163_AT

30
12166_I_AT

31
12167_AT

32
12169_I_AT

33
12175_AT

34
12176_AT

35
12179_AT

36
12187_AT

15920_I_AT

37
12195_AT

38
12196_AT

39
12198_AT

40
12200_AT

41
12202_AT

42
12214_G_AT

43
12219_AT

44
12224_AT

45
12226_AT

46
12233_AT

47
12240_AT

48
12253_G_AT

49
12256_AT

50
12269_S_AT

51
12270_AT

52
12284_AT

53
12287_S_AT

17570_G_AT

54
12293_AT

55
12294_S_AT

56
12300_AT

57
12307_AT

58
12312_AT

59
12315_AT

60
12324_I_AT

61
12331_S_AT

62
12336_AT

63
12344_AT

64
12348_AT

65
12353_AT

66
12359_S_AT

67
12372_AT

68
12374_I_AT

12726_F_AT

69
12390_AT

70
12395_S_AT

71
12405_AT

72
12408_AT

73
12410_G_AT

74
12419_AT

75
12427_AT

76
12431_AT

77
12436_AT

78
12438_AT

79
12443_S_AT

80
12447_AT

81
12450_S_AT

82
12452_AT

83
12474_AT

84
12477_AT

85
12491_AT

86
12497_AT

87
12500_S_AT

88
12503_AT

89
12515_AT

90
12516_S_AT

91
12523_AT

92
12526_AT

93
12527_AT

94
12532_AT

95
12534_G_AT

96
12544_AT

97
12549_S_AT

98
12550_S_AT

17103_S_AT

99
12552_AT

100
12555_S_AT

101
12576_S_AT

102
12581_S_AT

16645_S_AT

103
12587_AT

104
12597_AT

105
12602_AT

106
12610_AT

107
12631_AT

108
12646_AT

109
12649_AT

110
12650_AT

111
12653_AT

112
12661_AT

113
12666_AT

114
12674_AT

115
12675_S_AT

116
12678_I_AT

117
12681_S_AT

118
12688_AT

119
12702_AT

120
12705_F_AT

121
12736_F_AT

122
12737_F_AT

123
12758_AT

124
12760_G_AT

125
12762_R_AT

126
12764_F_AT

127
12766_AT

15115_F_AT

128
12767_AT

129
12768_AT

130
12772_AT

131
12773_AT

132
12776_AT

133
12788_AT

134
12793_AT

135
12794_AT

136
12802_AT

137
12809_G_AT

138
12812_AT

139
12815_AT

140
12816_AT

141
12818_AT

142
12824_S_AT

143
12828_S_AT

144
12842_S_AT

145
12846_S_AT

146
12858_AT

147
12860_S_AT

148
12861_S_AT

149
12881_S_AT

17600_S_AT

150
12889_S_AT

151
12901_S_AT

152
12902_AT

153
12904_S_AT

154
12905_S_AT

155
12908_S_AT

156
12910_S_AT

16385_S_AT

157
12914_S_AT

15783_S_AT

17645_S_AT

158
12916_S_AT

159
12923_S_AT

160
12926_S_AT

161
12927_S_AT

162
12931_S_AT

163
12937_R_AT

164
12941_G_AT

165
12942_AT

166
12947_AT

167
12949_AT

168
12953_AT

169
12956_I_AT

170
12959_AT

171
12966_S_AT

172
12975_AT

173
12983_AT

174
12984_AT

175
12987_S_AT

176
12994_S_AT

177
13002_AT

178
13009_I_AT

179
13011_AT

180
13018_AT

181
13023_AT

182
13024_AT

183
13034_S_AT

184
13046_G_AT

185
13048_S_AT

13495_S_AT

186
13054_AT

187
13067_S_AT

188
13068_AT

189
13073_S_AT

190
13078_S_AT

191
13079_AT

192
13081_S_AT

193
13083_AT

194
13086_R_AT

195
13087_AT

196
13090_AT

197
13092_S_AT

16950_S_AT

198
13098_AT

199
13100_AT

200
13103_AT

201
13105_AT

202
13107_S_AT

203
13108_AT

204
13109_AT

205
13114_AT

206
13118_F_AT

207
13119_AT

208
13120_AT

209
13123_AT

210
13128_AT

211
13133_S_AT

17430_S_AT

212
13135_S_AT

213
13139_AT

214
13140_AT

215
13143_AT

216
13151_G_AT

217
13160_AT

218
13161_AT

219
13162_AT

220
13165_AT

221
13166_AT

222
13167_AT

223
13179_AT

224
13181_AT

225
13185_AT

226
13193_S_AT

227
13213_S_AT

16004_S_AT

228
13219_S_AT

20288_G_AT

229
13220_S_AT

13221_AT

18929_S_AT

230
13233_AT

14301_S_AT

231
13243_R_AT

232
13254_S_AT

233
13260_S_AT

15660_S_AT

234
13273_S_AT

16105_S_AT

235
13274_S_AT

17077_S_AT

236
13276_S_AT

237
13278_F_AT

238
13285_S_AT

239
13288_S_AT

17043_S_AT

240
13292_S_AT

241
13296_S_AT

242
13297_S_AT

243
13299_S_AT

15166_S_AT

244
13332_AT

245
13347_AT

246
13351_AT

247
13352_AT

248
13355_AT

249
13404_AT

250
13422_AT

251
13459_AT

252
13460_AT

253
13461_S_AT

254
13467_AT

255
13488_AT

256
13523_S_AT

257
13529_AT

258
13539_I_AT

14631_S_AT

259
13541_AT

260
13542_AT

261
13545_S_AT

262
13552_AT

263
13556_I_AT

264
13561_AT

265
13563_S_AT

266
13567_AT

267
13568_AT

268
13571_AT

269
13575_AT

270
13576_AT

271
13583_AT

272
13598_AT

273
13601_AT

274
13604_AT

275
13613_AT

276
13616_S_AT

16544_S_AT

277
13617_AT

278
13618_S_AT

279
13619_AT

280
13621_G_AT

281
13623_R_AT

282
13629_S_AT

283
13631_AT

284
13635_AT

285
13646_AT

286
13650_AT

287
13653_AT

288
13655_AT

289
13656_AT

290
13657_AT

291
13666_S_AT

17083_S_AT

292
13667_S_AT

293
13669_S_AT

17074_S_AT

294
13670_S_AT

15206_S_AT

295
13671_S_AT

16805_S_AT

296
13678_S_AT

297
13688_S_AT

298
13690_S_AT

16065_S_AT

299
13691_S_AT

16117_S_AT

300
13692_S_AT

16118_S_AT

301
13700_AT

302
13704_S_AT

303
13714_AT

304
13715_AT

305
13724_AT

306
13748_AT

307
13759_AT

308
13767_AT

309
13785_AT

310
13803_AT

311
13850_I_AT

312
13876_AT

313
13880_S_AT

314
13883_AT

315
13887_S_AT

316
13895_AT

317
13904_S_AT

18722_S_AT

318
13906_S_AT

319
13908_S_AT

18597_AT

320
13923_AT

321
13927_AT

322
13932_AT

323
13935_AT

324
13940_AT

325
13949_S_AT

326
13954_G_AT

327
13971_S_AT

328
13973_AT

329
13983_AT

330
13985_S_AT

331
13987_S_AT

18738_F_AT

332
13989_AT

20674_S_AT

333
14010_AT

334
14013_AT

335
14014_AT

336
14019_AT

337
14021_R_AT

338
14025_S_AT

18909_S_AT

339
14027_AT

340
14030_AT

341
14044_AT

342
14048_AT

343
14056_AT

344
14057_AT

345
14058_AT

346
14059_AT

347
14061_AT

348
14068_S_AT

349
14072_AT

350
14073_AT

351
14074_AT

352
14084_AT

353
14095_S_AT

354
14100_AT

355
14101_AT

356
14103_AT

357
14105_AT

358
14106_AT

359
14121_AT

360
14129_S_AT

361
14133_S_AT

362
14143_AT

363
14145_AT

364
14148_AT

365
14186_AT

366
14194_AT

367
14196_AT

368
14223_AT

369
14234_AT

370
14236_AT

371
14251_F_AT

372
14252_F_AT

373
14270_AT

374
14298_G_AT

17581_G_AT

375
14303_S_AT

376
14312_AT

377
14316_AT

378
14339_AT

379
14366_AT

380
14369_AT

381
14388_AT

382
14392_G_AT

383
14393_AT

384
14421_AT

385
14436_AT

386
14448_AT

387
14450_AT

388
14454_AT

389
14459_AT

390
14478_AT

391
14482_AT

392
14485_AT

393
14492_S_AT

394
14505_AT

395
14510_AT

396
14511_AT

397
14517_AT

398
14519_AT

399
14525_S_AT

400
14527_AT

401
14534_S_AT

402
14538_R_AT

403
14554_AT

404
14558_AT

405
14559_S_AT

406
14566_AT

407
14572_AT

408
14579_AT

409
14587_AT

410
14591_AT

411
14595_AT

412
14602_AT

413
14603_AT

414
14605_AT

415
14620_S_AT

416
14626_S_AT

417
14630_S_AT

16559_S_AT

418
14637_S_AT

17122_S_AT

419
14642_F_AT

420
14650_S_AT

15150_S_AT

421
14654_S_AT

422
14667_S_AT

18299_S_AT

423
14669_S_AT

16136_S_AT

424
14672_S_AT

425
14679_S_AT

426
14682_I_AT

427
14689_AT

428
14697_G_AT

16902_AT

429
14701_S_AT

14734_S_AT

430
14703_AT

431
14711_S_AT

432
14712_S_AT

20530_S_AT

433
14713_S_AT

434
14715_S_AT

435
14728_S_AT

436
14731_S_AT

437
14781_AT

438
14797_S_AT

439
14800_AT

440
14809_AT

441
14843_AT

442
14847_AT

443
14872_AT

444
14886_AT

445
14896_AT

446
14900_AT

447
14908_AT

448
14912_AT

449
14914_AT

450
14942_AT

451
14945_AT

452
14955_AT

453
14957_S_AT

454
14958_AT

455
14965_AT

456
14974_AT

457
14980_AT

458
14981_AT

459
14984_S_AT

460
14995_AT

461
15004_AT

462
15009_AT

463
15010_AT

464
15024_AT

465
15026_AT

466
15036_R_AT

467
15054_AT

468
15056_AT

469
15057_AT

470
15066_AT

471
15073_AT

472
15081_AT

473
15083_AT

474
15091_AT

475
15097_S_AT

476
15101_S_AT

477
15102_S_AT

478
15107_S_AT

479
15112_S_AT

480
15116_F_AT

481
15118_S_AT

482
15122_S_AT

483
15130_S_AT

484
15131_S_AT

485
15132_S_AT

17585_S_AT

486
15139_S_AT

487
15143_S_AT

488
15146_S_AT

489
15159_S_AT

15160_S_AT

490
15162_S_AT

491
15167_S_AT

492
15171_S_AT

493
15174_F_AT

494
15178_S_AT

495
15185_S_AT

18023_S_AT

496
15188_S_AT

497
15193_S_AT

498
15196_S_AT

499
15197_S_AT

500
15201_F_AT

501
15213_S_AT

502
15243_AT

503
15256_AT

504
15270_AT

505
15319_AT

506
15325_AT

507
15337_AT

508
15341_AT

509
15343_AT

510
15348_AT

511
15350_AT

512
15355_S_AT

513
15367_AT

514
15372_AT

515
15379_AT

516
15381_AT

517
15383_AT

518
15384_AT

519
15385_AT

520
15387_AT

521
15410_AT

522
15417_S_AT

523
15422_AT

524
15423_AT

525
15431_AT

526
15433_AT

527
15452_AT

528
15464_AT

529
15468_AT

530
15471_AT

531
15472_AT

532
15475_S_AT

533
15485_AT

534
15489_AT

535
15490_AT

536
15503_AT

537
15505_AT

538
15510_R_AT

539
15512_AT

540
15514_AT

541
15515_R_AT

542
15517_S_AT

543
15518_AT

544
15529_AT

545
15534_F_AT

546
15538_AT

547
15541_AT

548
15543_AT

549
15544_AT

550
15551_AT

551
15574_S_AT

552
15576_S_AT

553
15577_S_AT

554
15578_S_AT

555
15583_S_AT

556
15588_S_AT

557
15595_S_AT

558
15600_S_AT

559
15602_F_AT

560
15608_S_AT

561
15613_S_AT

562
15616_S_AT

563
15618_S_AT

564
15620_S_AT

565
15627_S_AT

566
15634_S_AT

16125_S_AT

18046_S_AT

567
15637_S_AT

568
15639_S_AT

569
15642_S_AT

570
15643_S_AT

571
15651_F_AT

572
15652_S_AT

573
15665_S_AT

574
15667_S_AT

18610_S_AT

575
15668_S_AT

576
15671_S_AT

577
15675_S_AT

578
15679_S_AT

579
15685_S_AT

580
15687_F_AT

581
15688_S_AT

582
15689_S_AT

583
15692_S_AT

584
15694_S_AT

585
15712_S_AT

586
15808_AT

587
15845_AT

588
15848_AT

589
15850_AT

20406_G_AT

590
15858_AT

591
15862_AT

592
15868_AT

593
15878_AT

594
15894_AT

595
15900_AT

596
15901_AT

597
15902_AT

598
15912_AT

599
15913_AT

600
15928_AT

601
15940_AT

602
15941_AT

603
15945_AT

604
15948_S_AT

605
15956_AT

606
15960_AT

16466_S_AT

607
15976_AT

608
15978_AT

609
15986_S_AT

610
15990_AT

611
16009_S_AT

612
16015_AT

613
16019_AT

614
16024_AT

615
16034_AT

616
16036_I_AT

18729_AT

617
16039_S_AT

618
16040_AT

619
16042_S_AT

620
16047_AT

621
16049_S_AT

622
16051_S_AT

623
16055_S_AT

624
16059_S_AT

625
16062_S_AT

626
16066_S_AT

627
16069_S_AT

628
16074_S_AT

629
16076_S_AT

630
16077_S_AT

17579_S_AT

631
16079_S_AT

632
16084_S_AT

17998_S_AT

633
16087_S_AT

634
16089_S_AT

635
16090_S_AT

636
16102_S_AT

637
16103_S_AT

638
16108_S_AT

639
16112_S_AT

640
16134_S_AT

641
16137_S_AT

642
16138_S_AT

643
16140_S_AT

644
16143_S_AT

645
16145_S_AT

646
16148_S_AT

647
16151_S_AT

648
16155_S_AT

649
16158_F_AT

650
16160_F_AT

651
16162_S_AT

652
16168_S_AT

653
16169_S_AT

654
16171_S_AT

655
16172_S_AT

656
16184_AT

657
16192_AT

658
16222_AT

659
16242_AT

660
16244_AT

661
16250_AT

662
16286_AT

663
16288_AT

664
16294_S_AT

665
16296_AT

666
16297_AT

667
16325_AT

668
16346_S_AT

669
16357_AT

670
16380_AT

671
16382_AT

672
16393_S_AT

673
16402_S_AT

674
16411_S_AT

675
16442_S_AT

676
16446_AT

677
16448_G_AT

678
16453_S_AT

679
16457_S_AT

680
16465_AT

16916_S_AT

681
16470_S_AT

18735_S_AT

682
16481_S_AT

683
16486_AT

684
16487_AT

685
16488_AT

686
16496_S_AT

687
16499_AT

688
16510_AT

689
16511_AT

690
16512_S_AT

18085_R_AT

691
16514_AT

692
16516_AT

693
16517_AT

694
16526_AT

695
16528_AT

696
16531_S_AT

697
16535_S_AT

698
16537_S_AT

699
16538_S_AT

700
16543_S_AT

701
16550_S_AT

702
16554_S_AT

703
16567_S_AT

704
16571_S_AT

705
16576_F_AT

706
16577_S_AT

707
16579_S_AT

708
16580_S_AT

709
16583_S_AT

710
16584_S_AT

18706_S_AT

711
16593_S_AT

712
16595_S_AT

713
16598_S_AT

714
16604_S_AT

715
16605_S_AT

716
16610_S_AT

717
16611_S_AT

718
16614_S_AT

719
16617_S_AT

720
16618_S_AT

721
16620_S_AT

722
16621_S_AT

723
16631_S_AT

724
16634_S_AT

725
16635_S_AT

726
16636_S_AT

727
16639_S_AT

728
16640_S_AT

729
16650_S_AT

730
16652_S_AT

731
16654_AT

732
16672_AT

733
16673_AT

734
16687_S_AT

735
16747_AT

736
16753_AT

737
16768_AT

738
16777_AT

739
16784_AT

740
16807_AT

741
16811_AT

742
16845_AT

743
16894_AT

744
16899_AT

745
16911_AT

746
16920_AT

747
16921_AT

748
16924_S_AT

749
16926_S_AT

750
16931_S_AT

751
16934_S_AT

752
16937_AT

753
16938_AT

754
16942_AT

755
16943_S_AT

18231_AT

756
16949_S_AT

757
16952_S_AT

758
16956_AT

759
16962_S_AT

760
16965_S_AT

761
16970_S_AT

18010_S_AT

762
16977_AT

763
16984_AT

764
16996_S_AT

765
16997_AT

766
17000_AT

767
17005_AT

768
17010_S_AT

769
17017_S_AT

770
17031_S_AT

771
17033_S_AT

772
17053_S_AT

773
17055_S_AT

774
17063_S_AT

775
17068_S_AT

776
17070_S_AT

777
17075_S_AT

778
17084_S_AT

779
17087_S_AT

780
17092_S_AT

781
17095_S_AT

782
17096_S_AT

783
17102_S_AT

784
17105_S_AT

785
17109_S_AT

786
17110_S_AT

787
17113_S_AT

788
17115_S_AT

789
17116_S_AT

790
17123_S_AT

791
17129_S_AT

792
17132_AT

793
17166_AT

794
17206_AT

795
17207_AT

796
17215_AT

797
17237_AT

798
17247_AT

799
17254_AT

800
17286_AT

801
17288_S_AT

802
17292_AT

803
17300_AT

804
17303_S_AT

805
17318_AT

806
17319_AT

807
17322_AT

808
17323_AT

809
17332_S_AT

810
17374_AT

811
17381_AT

812
17388_AT

813
17392_S_AT

814
17405_AT

815
17415_AT

816
17418_S_AT

817
17420_AT

818
17423_S_AT

819
17426_AT

820
17427_AT

821
17429_S_AT

822
17431_AT

823
17439_G_AT

824
17457_AT

825
17458_AT

826
17462_S_AT

827
17463_AT

828
17465_AT

829
17466_S_AT

830
17475_AT

831
17479_AT

832
17482_S_AT

833
17495_S_AT

834
17508_S_AT

835
17522_S_AT

836
17523_S_AT

837
17537_S_AT

838
17538_S_AT

839
17539_S_AT

840
17546_S_AT

18694_S_AT

841
17557_S_AT

842
17560_S_AT

843
17562_AT

844
17564_S_AT

19361_S_AT

845
17565_S_AT

846
17568_AT

847
17573_AT

848
17577_G_AT

849
17578_AT

850
17596_AT

851
17627_AT

852
17631_AT

853
17632_AT

854
17672_AT

855
17675_AT

856
17677_AT

857
17732_AT

858
17743_AT

859
17748_AT

860
17782_AT

861
17823_S_AT

862
17841_AT

863
17849_S_AT

864
17852_G_AT

865
17857_AT

866
17865_AT

867
17882_AT

868
17885_AT

869
17900_S_AT

870
17910_AT

871
17911_AT

872
17916_AT

873
17917_S_AT

874
17918_AT

875
17921_S_AT

876
17922_AT

877
17926_S_AT

878
17933_AT

879
17935_AT

880
17956_I_AT

881
17966_AT

882
17967_AT

883
17970_I_AT

884
17978_S_AT

20635_S_AT

885
17986_S_AT

886
17993_AT

887
18001_AT

888
18003_AT

889
18004_AT

890
18005_AT

891
18029_G_AT

18030_I_AT

892
18040_S_AT

893
18045_AT

894
18064_R_AT

895
18065_R_AT

896
18074_AT

897
18076_S_AT

898
18077_AT

899
18081_AT

900
18154_S_AT

18365_S_AT

901
18165_AT

902
18174_AT

903
18176_AT

904
18194_I_AT

905
18197_AT

906
18198_AT

907
18213_AT

908
18219_AT

909
18221_AT

910
18222_AT

911
18226_S_AT

912
18232_AT

913
18237_AT

914
18241_AT

915
18257_AT

916
18258_S_AT

917
18269_S_AT

918
18274_S_AT

919
18275_AT

920
18278_AT

921
18282_AT

922
18283_AT

923
18290_AT

924
18291_AT

925
18306_AT

926
18316_AT

927
18317_AT

928
18327_S_AT

929
18337_S_AT

930
18339_AT

931
18347_S_AT

932
18383_AT

933
18390_AT

934
18439_S_AT

935
18465_S_AT

936
18487_AT

937
18508_S_AT

938
18512_AT

939
18543_AT

940
18544_AT

941
18552_AT

942
18555_AT

943
18556_AT

944
18561_AT

945
18567_AT

946
18573_AT

947
18580_AT

948
18581_AT

949
18584_AT

950
18587_S_AT

951
18588_AT

952
18591_AT

953
18592_S_AT

954
18600_AT

955
18601_S_AT

956
18607_S_AT

957
18611_AT

958
18616_AT

959
18622_G_AT

960
18623_AT

961
18628_AT

962
18631_AT

963
18635_AT

964
18636_AT

965
18638_AT

966
18652_AT

967
18657_AT

968
18659_AT

969
18660_S_AT

970
18667_AT

971
18675_AT

972
18684_AT

973
18686_S_AT

974
18688_S_AT

975
18693_S_AT

976
18698_S_AT

977
18705_AT

978
18707_AT

979
18708_AT

980
18726_S_AT

981
18727_AT

982
18732_I_AT

983
18736_AT

984
18750_F_AT

985
18754_AT

986
18778_AT

987
18806_S_AT

988
18823_S_AT

989
18829_AT

990
18835_AT

991
18844_AT

992
18859_AT

993
18864_AT

994
18866_AT

995
18880_AT

996
18883_G_AT

997
18885_AT

998
18886_AT

999
18887_AT

1000
18888_AT

1001
18889_AT

1002
18892_S_AT

1003
18901_AT

1004
18911_AT

1005
18917_I_AT

1006
18939_AT

1007
18947_I_AT

1008
18950_AT

1009
18951_S_AT

1010
18954_AT

1011
18956_AT

1012
18959_AT

1013
18966_AT

1014
18974_AT

1015
18976_AT

1016
18980_AT

1017
18989_S_AT

1018
18994_AT

1019
19030_AT

1020
19039_AT

1021
19049_AT

1022
19083_AT

1023
19115_AT

1024
19117_S_AT

1025
19122_AT

1026
19125_S_AT

1027
19127_AT

1028
19130_AT

1029
19144_AT

1030
19157_S_AT

1031
19178_AT

1032
19190_G_AT

1033
19198_AT

1034
19202_AT

1035
19209_S_AT

1036
19211_AT

1037
19218_AT

1038
19222_AT

1039
19226_G_AT

1040
19229_AT

1041
19230_AT

1042
19232_S_AT

1043
19285_AT

1044
19326_AT

1045
19332_AT

1046
19346_AT

1047
19347_AT

1048
19362_AT

1049
19363_AT

1050
19364_AT

1051
19367_AT

1052
19373_AT

1053
19381_AT

1054
19382_AT

1055
19384_AT

1056
19401_AT

1057
19406_AT

1058
19413_AT

1059
19416_AT

1060
19426_S_AT

1061
19439_AT

1062
19441_S_AT

1063
19442_AT

1064
19448_S_AT

1065
19454_AT

1066
19462_S_AT

1067
19464_AT

1068
19470_AT

1069
19483_AT

1070
19489_S_AT

1071
19513_AT

1072
19548_AT

1073
19562_AT

1074
19563_S_AT

1075
19567_AT

1076
19581_AT

1077
19589_S_AT

1078
19595_S_AT

1079
19606_AT

1080
19623_AT

1081
19624_AT

1082
19627_S_AT

1083
19636_AT

1084
19652_AT

1085
19655_AT

1086
19657_S_AT

1087
19658_AT

1088
19660_AT

1089
19665_S_AT

1090
19667_AT

1091
19671_AT

1092
19677_AT

1093
19686_AT

1094
19689_AT

1095
19690_S_AT

1096
19695_AT

1097
19698_AT

1098
19700_S_AT

1099
19708_AT

1100
19717_AT

1101
19726_S_AT

1102
19744_AT

1103
19752_S_AT

1104
19759_AT

1105
19782_AT

1106
19803_S_AT

1107
19828_AT

1108
19831_I_AT

1109
19833_S_AT

1110
19834_AT

1111
19836_AT

1112
19841_AT

1113
19845_G_AT

1114
19854_AT

1115
19855_AT

1116
19866_AT

1117
19867_AT

1118
19870_S_AT

1119
19871_AT

1120
19872_AT

1121
19875_S_AT

1122
19876_AT

1123
19879_S_AT

1124
19881_AT

1125
19897_S_AT

1126
19903_AT

1127
19905_AT

1128
19906_AT

1129
19907_AT

1130
19910_AT

1131
19913_AT

1132
19920_S_AT

1133
19932_AT

1134
19939_AT

1135
19945_AT

1136
19947_AT

1137
19951_AT

1138
19956_AT

1139
19962_AT

1140
19963_AT

1141
19969_AT

1142
19970_S_AT

1143
19971_AT

1144
19972_AT

1145
19981_AT

1146
19990_AT

1147
19996_AT

1148
20003_S_AT

1149
20009_S_AT

1150
20013_AT

1151
20018_AT

1152
20024_S_AT

1153
20027_AT

1154
20045_AT

1155
20047_AT

1156
20048_AT

1157
20050_AT

1158
20051_AT

1159
20058_AT

1160
20067_AT

1161
20068_AT

1162
20069_AT

1163
20093_I_AT

1164
20099_AT

1165
20100_AT

1166
20113_S_AT

1167
20117_AT

1168
20123_AT

1169
20127_S_AT

1170
20129_AT

1171
20150_AT

1172
20154_AT

1173
20156_AT

1174
20165_AT

1175
20173_AT

1176
20178_S_AT

1177
20183_AT

1178
20188_AT

1179
20189_AT

1180
20197_AT

1181
20210_G_AT

1182
20213_AT

1183
20229_AT

1184
20232_S_AT

1185
20255_AT

1186
20257_AT

1187
20262_AT

1188
20275_AT

1189
20278_S_AT

1190
20282_S_AT

1191
20284_AT

1192
20293_AT

1193
20294_AT

1194
20312_S_AT

1195
20315_I_AT

1196
20330_S_AT

1197
20331_AT

1198
20350_S_AT

1199
20354_S_AT

1200
20355_AT

1201
20360_AT

1202
20363_AT

1203
20369_S_AT

1204
20378_G_AT

1205
20383_AT

1206
20384_AT

1207
20387_AT

1208
20393_AT

1209
20396_AT

1210
20399_AT

1211
20409_G_AT

1212
20412_S_AT

1213
20413_AT

1214
20439_AT

1215
20440_AT

1216
20444_AT

1217
20445_AT

1218
20449_AT

1219
20456_AT

1220
20462_AT

1221
20471_AT

1222
20474_AT

1223
20495_S_AT

1224
20499_AT

1225
20501_AT

1226
20511_AT

1227
20515_S_AT

1228
20516_AT

1229
20517_AT

1230
20518_AT

1231
20520_S_AT

1232
20536_S_AT

1233
20538_S_AT

1234
20539_S_AT

1235
20558_AT

1236
20561_AT

1237
20567_AT

1238
20571_AT

1239
20582_S_AT

1240
20586_I_AT

1241
20590_AT

1242
20592_AT

1243
20594_AT

1244
20608_S_AT

1245
20612_S_AT

1246
20616_AT

1247
20620_G_AT

1248
20637_AT

1249
20643_AT

1250
20649_AT

1251
20651_AT

1252
20654_S_AT

1253
20670_AT

1254
20684_AT

1255
20685_AT

1256
20693_AT

1257
20701_S_AT

1258
20704_AT

1259
20705_AT

1260
20715_AT

1261
20719_AT

TABLE 4

2X UP IN COLD, ONLY

11997_at
12688_at
13274_s_at
14145_at
15083_at
15639_s_at

11998_at
12701_i_at
13278_f_at
14170_at
15084_at
15641_s_at

12018_at
12702_at
13279_s_at
14186_at
15096_at
15660_s_at

12031_at
12719_f_at
13285_s_at
14196_at
15101_s_at
15665_s_at

12047_at
12726_f_at
13288_s_at
14227_at
15105_s_at
15687_f_at

12051_at
12736_f_at
13292_s_at
14234_at
15112_s_at
15694_s_at

12053_at
12754_g_at
13297_s_at
14250_r_at
15115_f_at
15712_s_at

12060_at
12762_r_at
13299_s_at
14270_at
15116_f_at
15783_s_at

12072_at
12766_at
13332_at
14298_g_at
15122_s_at
15808_at

12074_at
12767_at
13351_at
14303_s_at
15126_s_at
15837_at

12102_at
12768_at
13352_at
14312_at
15131_s_at
15850_at

12112_at
12773_at
13422_at
14339_at
15132_s_at
15862_at

12117_at
12788_at
13435_at
14388_at
15137_s_at
15868_at

12130_at
12802_at
13461_s_at
14393_at
15144_s_at
15878_at

12145_s_at
12860_s_at
13467_at
14511_at
15148_s_at
15901_at

12151_at
12861_s_at
13488_at
14525_s_at
15153_s_at
15912_at

12163_at
12879_s_at
13495_s_at
14527_at
15159_s_at
15920_i_at

12175_at
12891_at
13539_i_at
14534_s_at
15160_s_at
15941_at

12187_at
12914_s_at
13542_at
14554_at
15166_s_at
15945_at

12195_at
12927_s_at
13575_at
14566_at
15174_f_at
15960_at

12219_at
12947_at
13577_s_at
14579_at
15197_s_at
15990_at

12256_at
12956_i_at
13617_at
14591_at
15270_at
16001_at

12269_s_at
12966_s_at
13634_s_at
14595_at
15319_at
16009_s_at

12307_at
12974_at
13656_at
14600_at
15325_at
16010_s_at

12315_at
12987_s_at
13671_s_at
14631_s_at
15337_at
16034_at

12336_at
12994_s_at
13691_s_at
14635_s_at
15341_at
16036_i_at

12349_s_at
12998_at
13700_at
14679_s_at
15343_at
16039_s_at

12353_at
13002_at
13704_s_at
14691_at
15355_s_at
16040_at

12359_s_at
13018_at
13709_s_at
14697_g_at
15367_at
16042_s_at

12390_at
13023_at
13715_at
14709_at
15379_at
16047_at

12395_s_at
13046_g_at
13785_at
14711_s_at
15381_at
16049_s_at

12431_at
13054_at
13803_at
14728_s_at
15410_at
16051_s_at

12436_at
13086_r_at
13812_s_at
14731_s_at
15417_s_at
16062_s_at

12443_s_at
13087_at
13825_s_at
14797_s_at
15422_at
16079_s_at

12447_at
13100_at
13850_i_at
14809_at
15433_at
16087_s_at

12452_at
13109_at
13904_s_at
14843_at
15451_at
16090_s_at

12477_at
13119_at
13908_s_at
14847_at
15452_at
16117_s_at

12503_at
13120_at
13927_at
14872_at
15453_s_at
16118_s_at

12516_s_at
13128_at
13971_s_at
14886_at
15472_at
16137_s_at

12532_at
13134_s_at
13985_s_at
14896_at
15489_at
16155_s_at

12544_at
13140_at
14013_at
14897_at
15490_at
16162_s_at

12561_at
13143_at
14019_at
14900_at
15503_at
16184_at

12602_at
13167_at
14021_r_at
14956_s_at
15510_r_at
16192_at

12610_at
13172_s_at
14028_at
14958_at
15517_s_at
16222_at

12631_at
13178_at
14048_at
14965_at
15518_at
16244_at

12647_s_at
13179_at
14058_at
14984_s_at
15544_at
16250_at

12650_at
13181_at
14059_at
15004_at
15588_s_at
16260_at

12656_at
13187_i_at
14064_at
15010_at
15600_s_at
16286_at

12674_at
13209_s_at
14073_at
15036_r_at
15605_s_at
16296_at

12675_s_at
13219_s_at
14105_at
15040_g_at
15613_s_at
16297_at

12676_s_at
13221_at
14106_at
15046_s_at
15614_s_at
16342_at

12681_s_at
13243_r_at
14126_s_at
15057_at
15616_s_at
16367_i_at

12686_s_at
13260_s_at
14140_at
15073_at
15633_s_at
16411_s_at

16442_s_at
17077_s_at
17978_s_at
18885_at
19689_at
20412_s_at

16465_at
17102_s_at
17999_at
18887_at
19698_at
20413_at

16466_s_at
17109_s_at
18001_at
18888_at
19700_s_at
20432_at

16468_at
17113_s_at
18004_at
18889_at
19707_s_at
20433_at

16486_at
17123_s_at
18012_s_at
18901_at
19708_at
20456_at

16487_at
17128_s_at
18040_s_at
18907_s_at
19713_at
20462_at

16488_at
17129_s_at
18176_at
18917_i_at
19718_at
20471_at

16489_at
17132_at
18194_i_at
18939_at
19744_at
20511_at

16496_s_at
17166_at
18197_at
18947_i_at
19836_at
20515_s_at

16499_at
17206_at
18198_at
18949_at
19839_at
20517_at

16511_at
17237_at
18213_at
18954_at
19840_s_at
20518_at

16517_at
17300_at
18219_at
18959_at
19845_g_at
20529_at

16538_s_at
17319_at
18222_at
18974_at
19854_at
20536_s_at

16554_s_at
17322_at
18231_at
18976_at
19855_at
20538_s_at

16571_s_at
17332_s_at
18232_at
18980_at
19860_at
20539_s_at

16576_f_at
17381_at
18241_at
18989_s_at
19866_at
20576_at

16595_s_at
17388_at
18269_s_at
19019_i_at
19871_at
20582_s_at

16605_s_at
17392_s_at
18272_at
19049_at
19875_s_at
20586_i_at

16610_s_at
17408_at
18282_at
19083_at
19879_s_at
20608_s_at

16620_s_at
17424_at
18298_at
19130_at
19881_at
20649_at

16621_s_at
17429_s_at
18316_at
19156_s_at
19913_at
20651_at

16635_s_at
17457_at
18317_at
19178_at
19939_at
20684_at

16636_s_at
17458_at
18331_s_at
19190_g_at
19945_at
20685_at

16638_s_at
17466_s_at
18347_s_at
19199_at
19947_at
20699_at

16650_s_at
17477_s_at
18383_at
19202_at
19951_at
20705_at

16672_at
17482_s_at
18390_at
19209_s_at
19956_at
20715_at

16673_at
17538_s_at
18455_at
19211_at
19971_at

16687_s_at
17546_s_at
18465_s_at
19218_at
19976_at

16747_at
17562_at
18544_at
19229_at
19998_at

16753_at
17581_g_at
18555_at
19322_at
20003_s_at

16768_at
17627_at
18556_at
19326_at
20015_at

16805_s_at
17631_at
18560_at
19359_s_at
20027_at

16807_at
17632_at
18561_at
19367_at
20051_at

16845_at
17645_s_at
18571_at
19384_at
20068_at

16847_at
17672_at
18588_at
19389_at
20093_i_at

16896_s_at
17675_at
18597_at
19397_at
20117_at

16899_at
17677_at
18601_s_at
19406_at
20150_at

16902_at
17693_at
18611_at
19426_s_at
20156_at

16911_at
17732_at
18623_at
19441_s_at
20165_at

16914_s_at
17743_at
18635_at
19442_at
20257_at

16943_s_at
17748_at
18659_at
19470_at
20262_at

16956_at
17775_at
18660_s_at
19489_s_at
20275_at

16996_s_at
17782_at
18673_at
19562_at
20282_s_at

17010_s_at
17841_at
18694_s_at
19577_at
20288_g_at

17016_s_at
17852_g_at
18705_at
19589_s_at
20293_at

17032_s_at
17900_s_at
18708_at
19597_s_at
20315_i_at

17033_s_at
17901_at
18738_f_at
19611_s_at
20330_s_at

17043_s_at
17911_at
18750_f_at
19624_at
20360_at

17050_s_at
17921_s_at
18778_at
19657_s_at
20363_at

17055_s_at
17922_at
18829_at
19667_at
20369_s_at

17068_s_at
17933_at
18835_at
19671_at
20384_at

17071_s_at
17967_at
18866_at
19677_at
20393_at

17075_s_at
17970_i_at
18875_s_at
19686_at
20396_at

TABLE 5

2X UP COLD 3 HR, ONLY

12117_at

12145_s_at

12151_at

12163_at

12187_at

12256_at

12315_at

12349_s_at

12353_at

12359_s_at

12544_at

12602_at

12610_at

12676_s_at

12686_s_at

12701_i_at

12702_at

12719_f_at

12736_f_at

12754_g_at

12766_at

12767_at

12768_at

12773_at

12788_at

12879_s_at

12891_at

12947_at

12966_s_at

12974_at

12994_s_at

13002_at

13100_at

13140_at

13167_at

13172_s_at

13179_at

13187_i_at

13219_s_at

13260_s_at

13278_f_at

13279_s_at

13285_s_at

13288_s_at

13292_s_at

13297_s_at

13351_at

13352_at

13435_at

13467_at

13488_at

13495_s_at

13656_at

13671_s_at

13691_s_at

13785_at

13803_at

13825_s_at

13904_s_at

14013_at

14021_r_at

14028_at

14064_at

14126_s_at

14145_at

14170_at

14196_at

14250_r_at

14298_g_at

14303_s_at

14339_at

14527_at

14534_s_at

14554_at

14595_at

14635_s_at

14679_s_at

14691_at

14697_g_at

14709_at

14728_s_at

14809_at

14896_at

14965_at

14984_s_at

15046_s_at

15083_at

15096_at

15105_s_at

15115_f_at

15116_f_at

15122_s_at

15126_s_at

15131_s_at

15132_s_at

15137_s_at

15153_s_at

15159_s_at

15160_s_at

15197_s_at

15355_s_at

15379_at

15417_s_at

15422_at

15451_at

15452_at

15453_s_at

15489_at

15518_at

15588_s_at

15613_s_at

15614_s_at

15616_s_at

15639_s_at

15641_s_at

15660_s_at

15687_f_at

15694_s_at

15862_at

15868_at

15878_at

15901_at

16034_at

16039_s_at

16040_at

16042_s_at

16047_at

16062_s_at

16087_s_at

16117_s_at

16118_s_at

16162_s_at

16184_at

16222_at

16250_at

16411_s_at

16442_s_at

16465_at

16486_at

16488_at

16489_at

16517_at

16571_s_at

16605_s_at

16610_s_at

16620_s_at

16636_s_at

16650_s_at

16805_s_at

16845_at

16899_at

16914_s_at

16943_s_at

16996_s_at

17010_s_at

17043_s_at

17068_s_at

17109_s_at

17128_s_at

17237_at

17319_at

17392_s_at

17429_s_at

17477_s_at

17538_s_at

17581_g_at

17627_at

17672_at

17693_at

17782_at

17841_at

17900_s_at

17933_at

17978_s_at

18001_at

18012_s_at

18198_at

18219_at

18241_at

18269_s_at

18272_at

18282_at

18298_at

18383_at

18556_at

18588_at

18601_s_at

18611_at

18694_s_at

18708_at

18738_f_at

18778_at

18829_at

18835_at

18866_at

18875_s_at

18888_at

18907_s_at

18917_i_at

18939_at

18974_at

19190_g_at

19199_at

19202_at

19211_at

19384_at

19406_at

19426_s_at

19442_at

19470_at

19577_at

19597_s_at

19624_at

19657_s_at

19667_at

19845_g_at

19855_at

19866_at

19945_at

19951_at

19998_at

20003_s_at

20015_at

20051_at

20093_i_at

20117_at

20288_g_at

20360_at

20369_s_at

20384_at

20462_at

20471_at

20515_s_at

20538_s_at

20576_at

20608_s_at

20651_at

20685_at

20705_at

TABLE 6

2X DOWN COLD, ONLY

11991_g_at
12450_s_at
12881_s_at
13151_g_at
13621_g_at
14056_at

11992_at
12474_at
12889_s_at
13160_at
13623_r_at
14057_at

12001_at
12491_at
12901_s_at
13161_at
13629_s_at
14061_at

12006_s_at
12497_at
12902_at
13162_at
13631_at
14067_at

12007_at
12500_s_at
12904_s_at
13165_at
13635_at
14068_s_at

12009_at
12515_at
12905_s_at
13166_at
13646_at
14072_at

12022_at
12521_at
12908_s_at
13185_at
13650_at
14074_at

12023_s_at
12523_at
12910_s_at
13193_s_at
13652_at
14075_at

12026_at
12526_at
12916_s_at
13211_s_at
13653_at
14083_at

12037_at
12527_at
12923_s_at
13213_s_at
13655_at
14084_at

12052_at
12534_g_at
12926_s_at
13219_s_at
13657_at
14089_at

12125_at
12549_s_at
12931_s_at
13233_at
13666_s_at
14095_s_at

12143_at
12550_s_at
12937_r_at
13236_s_at
13667_s_at
14096_at

12149_at
12552_at
12941_g_at
13239_s_at
13669_s_at
14100_at

12156_at
12555_s_at
12942_at
13241_s_at
13670_s_at
14101_at

12166_i_at
12556_at
12949_at
13254_s_at
13672_s_at
14103_at

12167_at
12575_s_at
12953_at
13266_s_at
13678_s_at
14121_at

12169_i_at
12576_s_at
12958_at
13273_s_at
13679_s_at
14129_s_at

12176_at
12581_s_at
12959_at
13275_f_at
13688_s_at
14133_s_at

12179_at
12587_at
12966_s_at
13276_s_at
13690_s_at
14143_at

12196_at
12597_at
12975_at
13278_f_at
13691_s_at
14148_at

12198_at
12606_at
12983_at
13280_s_at
13692_s_at
14162_at

12200_at
12609_at
12984_at
13285_s_at
13714_at
14194_at

12202_at
12646_at
13002_at
13296_s_at
13724_at
14208_at

12212_at
12649_at
13009_i_at
13347_at
13748_at
14217_at

12214_g_at
12653_at
13011_at
13355_at
13751_at
14223_at

12224_at
12661_at
13014_at
13361_at
13759_at
14235_at

12226_at
12666_at
13024_at
13404_at
13767_at
14236_at

12233_at
12678_i_at
13034_s_at
13406_at
13789_at
14251_f_at

12240_at
12705_f_at
13041_s_at
13459_at
13876_at
14252_f_at

12253_g_at
12736_f_at
13048_s_at
13460_at
13880_s_at
14285_at

12270_at
12737_f_at
13067_s_at
13464_at
13883_at
14301_s_at

12278_at
12758_at
13068_at
13523_s_at
13887_s_at
14316_at

12284_at
12760_g_at
13073_s_at
13529_at
13895_at
14366_at

12287_s_at
12764_f_at
13078_s_at
13541_at
13906_s_at
14369_at

12293_at
12765_at
13079_at
13545_s_at
13919_at
14392_g_at

12294_s_at
12772_at
13081_s_at
13550_at
13923_at
14421_at

12300_at
12776_at
13083_at
13552_at
13932_at
14431_at

12312_at
12784_at
13090_at
13556_i_at
13935_at
14436_at

12315_at
12793_at
13092_s_at
13561_at
13940_at
14448_at

12324_i_at
12794_at
13098_at
13563_s_at
13949_s_at
14450_at

12331_s_at
12795_at
13103_at
13567_at
13954_g_at
14454_at

12344_at
12809_g_at
13105_at
13568_at
13973_at
14459_at

12348_at
12812_at
13107_s_at
13571_at
13983_at
14478_at

12353_at
12815_at
13108_at
13576_at
13989_at
14482_at

12372_at
12816_at
13114_at
13583_at
14010_at
14485_at

12374_i_at
12818_at
13118_f_at
13598_at
14014_at
14492_s_at

12405_at
12824_s_at
13123_at
13601_at
14015_s_at
14505_at

12408_at
12828_s_at
13124_at
13604_at
14016_s_at
14510_at

12410_g_at
12842_s_at
13133_s_at
13613_at
14025_s_at
14517_at

12419_at
12846_s_at
13135_s_at
13616_s_at
14027_at
14519_at

12427_at
12858_at
13139_at
13618_s_at
14030_at
14534_s_at

19438_at
12869_s_at
13146_s_at
13619_at
14044_at
14538_r_at

14558_at
15047_at
15512_at
15940_at
16357_at
16894_at

14559_s_at
15054_at
15514_at
15948_s_at
16380_at
16899_at

14572_at
15056_at
15515_r_at
15956_at
16382_at
16920_at

14584_at
15058_s_at
15529_at
15976_at
16385_s_at
16921_at

14587_at
15063_at
15534_f_at
15978_at
16393_s_at
16924_s_at

14595_at
15066_at
15538_at
15986_s_at
16402_s_at
16926_s_at

14602_at
15081_at
15541_at
16004_s_at
16417_s_at
16931_s_at

14603_at
15091_at
15543_at
16015_at
16442_s_at
16934_s_at

14605_at
15097_s_at
15551_at
16017_at
16446_at
16937_at

14620_s_at
15102_s_at
15574_s_at
16019_at
16448_g_at
16938_at

14626_s_at
15107_s_at
15576_s_at
16024_at
16453_s_at
16942_at

14630_s_at
15118_s_at
15577_s_at
16031_at
16457_s_at
16949_s_at

14637_s_at
15127_s_at
15578_s_at
16055_s_at
16470_s_at
16950_s_at

14640_s_at
15130_s_at
15581_s_at
16059_s_at
16481_s_at
16952_s_at

14642_f_at
15132_s_at
15583_s_at
16065_s_at
16510_at
16962_s_at

14650_s_at
15133_s_at
15591_s_at
16066_s_at
16512_s_at
16965_s_at

14654_s_at
15139_s_at
15595_s_at
16069_s_at
16514_at
16970_s_at

14667_s_at
15143_s_at
15602_f_at
16074_s_at
16516_at
16977_at

14668_s_at
15146_s_at
15606_s_at
16076_s_at
16523_s_at
16984_at

14669_s_at
15150_s_at
15608_s_at
16077_s_at
16526_at
16989_at

14672_s_at
15161_s_at
15616_s_at
16084_s_at
16528_at
16993_at

14673_s_at
15162_s_at
15618_s_at
16089_s_at
16531_s_at
16997_at

14675_s_at
15167_s_at
15620_s_at
16102_s_at
16535_s_at
17000_at

14679_s_at
15170_s_at
15627_s_at
16103_s_at
16537_s_at
17005_at

14681_g_at
15171_s_at
15634_s_at
16105_s_at
16543_s_at
17010_s_at

14682_i_at
15178_s_at
15637_s_at
16108_s_at
16544_s_at
17017_s_at

14689_at
15182_s_at
15642_s_at
16112_s_at
16550_s_at
17031_s_at

14701_s_at
15185_s_at
15643_s_at
16117_s_at
16559_s_at
17040_s_at

14703_at
15188_s_at
15646_s_at
16118_s_at
16567_s_at
17053_s_at

14712_s_at
15193_s_at
15651_f_at
16125_s_at
16577_s_at
17056_s_at

14713_s_at
15196_s_at
15652_s_at
16127_s_at
16579_s_at
17063_s_at

14715_s_at
15201_f_at
15667_s_at
16134_s_at
16580_s_at
17070_s_at

14734_s_at
15206_s_at
15668_s_at
16136_s_at
16583_s_at
17074_s_at

14781_at
15207_s_at
15670_s_at
16138_s_at
16584_s_at
17084_s_at

14800_at
15213_s_at
15671_s_at
16140_s_at
16593_s_at
17085_s_at

14856_s_at
15243_at
15675_s_at
16143_s_at
16598_s_at
17087_s_at

14882_at
15256_at
15679_s_at
16144_s_at
16603_s_at
17092_s_at

14908_at
15348_at
15685_s_at
16145_s_at
16604_s_at
17095_s_at

14912_at
15350_at
15688_s_at
16148_s_at
16611_s_at
17096_s_at

14914_at
15372_at
15689_s_at
16151_s_at
16614_s_at
17097_s_at

14924_at
15383_at
15692_s_at
16158_f_at
16617_s_at
17103_s_at

14942_at
15384_at
15775_at
16160_f_at
16618_s_at
17105_s_at

14945_at
15385_at
15776_at
16168_s_at
16620_s_at
17110_s_at

14955_at
15387_at
15845_at
16169_s_at
16631_s_at
17115_s_at

14957_s_at
15406_at
15848_at
16171_s_at
16634_s_at
17116_s_at

14974_at
15423_at
15858_at
16172_s_at
16639_s_at
17119_s_at

14980_at
15431_at
15866_s_at
16222_at
16640_s_at
17122_s_at

14981_at
15464_at
15894_at
16232_s_at
16652_s_at
17207_at

14995_at
15468_at
15900_at
16242_at
16654_at
17215_at

15009_at
15471_at
15901_at
16288_at
16777_at
17247_at

15018_at
15475_s_at
15902_at
16294_s_at
16784_at
17254_at

15024_at
15485_at
15913_at
16325_at
16811_at
17286_at

15026_at
15505_at
15928_at
16346_s_at
16893_at
17288_s_at

17292_at
17910_at
18337_s_at
18823_s_at
19382_at
19897_s_at

17303_s_at
17916_at
18339_at
18844_at
19401_at
19903_at

17305_at
17917_s_at
18365_s_at
18859_at
19402_at
19905_at

17318_at
17918_at
18402_at
18864_at
19406_at
19906_at

17323_at
17926_s_at
18439_s_at
18880_at
19413_at
19907_at

17374_at
17935_at
18487_at
18883_g_at
19416_at
19910_at

17405_at
17956_i_at
18508_s_at
18886_at
19429_at
19920_s_at

17415_at
17961_at
18512_at
18892_s_at
19432_s_at
19932_at

17418_s_at
17966_at
18543_at
18909_s_at
19439_at
19951_at

17420_at
17978_s_at
18552_at
18911_at
19448_s_at
19962_at

17423_s_at
17986_s_at
18567_at
18913_s_at
19454_at
19963_at

17426_at
17993_at
18573_at
18916_s_at
19462_s_at
19969_at

17427_at
17998_s_at
18580_at
18921_g_at
19464_at
19970_s_at

17430_s_at
18003_at
18581_at
18950_at
19469_at
19972_at

17431_at
18005_at
18584_at
18951_s_at
19483_at
19981_at

17439_g_at
18010_s_at
18587_s_at
18956_at
19484_s_at
19990_at

17442_i_at
18013_r_at
18590_at
18966_at
19513_at
19996_at

17449_s_at
18023_s_at
18591_at
18972_at
19548_at
19999_s_at

17462_s_at
18029_g_at
18592_s_at
18994_at
19563_s_at
20009_s_at

17463_at
18030_i_at
18600_at
19030_at
19567_at
20013_at

17465_at
18045_at
18601_s_at
19039_at
19581_at
20017_at

17475_at
18046_s_at
18607_s_at
19068_i_at
19595_s_at
20018_at

17479_at
18059_i_at
18610_s_at
19108_at
19606_at
20024_s_at

17495_s_at
18064_r_at
18611_at
19115_at
19623_at
20045_at

17508_s_at
18065_r_at
18616_at
19117_s_at
19627_s_at
20047_at

17522_s_at
18074_at
18622_g_at
19122_at
19636_at
20048_at

17523_s_at
18076_s_at
18628_at
19125_s_at
19641_at
20050_at

17529_s_at
18077_at
18631_at
19127_at
19652_at
20051_at

17537_s_at
18078_at
18636_at
19135_at
19655_at
20058_at

17539_s_at
18081_at
18638_at
19144_at
19658_at
20067_at

17543_s_at
18083_r_at
18652_at
19157_s_at
19660_at
20069_at

17555_s_at
18085_r_at
18657_at
19158_at
19665_s_at
20099_at

17557_s_at
18091_at
18667_at
19177_at
19667_at
20100_at

17560_s_at
18154_s_at
18675_at
19192_at
19690_s_at
20113_s_at

17564_s_at
18165_at
18684_at
19198_at
19695_at
20123_at

17565_s_at
18174_at
18686_s_at
19222_at
19717_at
20127_s_at

17568_at
18221_at
18688_s_at
19226_g_at
19726_s_at
20129_at

17570_g_at
18226_s_at
18693_s_at
19227_at
19752_s_at
20133_i_at

17573_at
18230_at
18698_s_at
19230_at
19759_at
20152_at

17577_g_at
18237_at
18706_s_at
19232_s_at
19782_at
20154_at

17578_at
18255_at
18707_at
19263_at
19789_s_at
20173_at

17579_s_at
18257_at
18726_s_at
19285_at
19803_s_at
20178_s_at

17585_s_at
18258_s_at
18727_at
19332_at
19828_at
20183_at

17596_at
18274_s_at
18732_i_at
19346_at
19831_i_at
20188_at

17600_s_at
18275_at
18735_s_at
19347_at
19833_s_at
20189_at

17823_s_at
18278_at
18736_at
19361_s_at
19834_at
20197_at

17840_s_at
18283_at
18738_f_at
19362_at
19835_at
20200_at

17849_s_at
18290_at
18747_f_at
19363_at
19841_at
20210_g_at

17857_at
18291_at
18754_at
19364_at
19867_at
20213_at

17865_at
18299_s_at
18782_at
19365_s_at
19870_s_at
20229_at

17882_at
18300_at
18789_at
19373_at
19871_at
20232_s_at

17885_at
18306_at
18806_s_at
19379_at
19872_at
20255_at

17902_s_at
18327_s_at
18814_at
19381_at
19876_at
20278_s_at

20284_at
20693_at

20288_g_at
20701_s_at

20294_at
20704_at

20312_s_at
20707_s_at

20331_at
20719_at

20335_s_at

20350_s_at

20354_s_at

20355_at

20369_s_at

20378_g_at

20383_at

20385_s_at

20387_at

20399_at

20409_g_at

20420_at

20429_s_at

20439_at

20440_at

20444_at

20445_at

20449_at

20474_at

20480_s_at

20495_s_at

20499_at

20501_at

20516_at

20520_s_at

20530_s_at

20538_s_at

20547_at

20558_at

20561_at

20567_at

20571_at

20590_at

20592_at

20594_at

20608_s_at

20612_s_at

20616_at

20620_g_at

20635_s_at

20637_at

20643_at

20654_s_at

20670_at

20674_s_at

20684_at

20685_at

20689_s_at

TABLE 7

SALINE STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX

ID NO:
ID NO:

2227
12011_S_AT

2228
12153_AT

2229
12180_AT

2230
12186_AT

2231
12216_AT

2232
12265_AT

2233
12335_AT

2234
12449_S_AT

2235
12470_AT

2236
12479_AT

2237
12487_AT

2238
12493_G_AT

2239
12562_AT

2240
12685_AT

2241
12704_F_AT

2242
12709_F_AT

2243
12734_F_AT

2244
12739_S_AT

2245
12750_S_AT

2246
12761_S_AT

2247
12813_AT

2248
12845_S_AT

2249
12946_AT

2250
13003_S_AT

2251
13052_S_AT

2252
13094_AT

2253
13142_AT

2254
13172_S_AT

17880_S_AT

2255
13198_I_AT

2256
13209_S_AT

16165_S_AT

2257
13229_S_AT

2258
13253_F_AT

2259
13344_S_AT

2260
13370_AT

2261
13387_AT

2262
13408_S_AT

2263
13429_AT

2264
13472_AT

2265
13526_AT

2266
13569_AT

2267
13614_AT

2268
13686_S_AT

2269
13718_AT

2270
13719_AT

2271
13902_AT

2272
13918_AT

2273
13944_AT

2274
13964_AT

2275
13993_S_AT

2276
14000_AT

2277
14003_AT

2278
14032_AT

2279
14043_AT

2280
14070_AT

2281
14267_AT

2282
14269_AT

2283
14418_AT

2284
14427_AT

2285
14501_AT

2286
14544_AT

2287
14546_S_AT

2288
14570_AT

2289
14596_AT

2290
14729_S_AT

2291
14874_AT

2292
14888_AT

2293
14951_AT

2294
14952_AT

2295
14959_AT

2296
14979_AT

2297
15006_AT

2298
15042_AT

2299
15049_AT

2300
15062_AT

2301
15108_S_AT

2302
15147_S_AT

2303
15175_S_AT

2304
15176_S_AT

2305
15186_S_AT

18696_S_AT

2306
15192_S_AT

2307
15208_S_AT

2308
15324_AT

2309
15371_AT

2310
15424_AT

2311
15463_AT

2312
15465_AT

2313
15497_S_AT

2314
15589_S_AT

2315
15636_S_AT

2316
15663_S_AT

2317
15770_AT

2318
15792_AT

2319
15855_AT

2320
15860_AT

2321
15891_AT

2322
15898_AT

2323
15909_AT

2324
15965_AT

2325
15969_S_AT

2326
15975_S_AT

2327
15995_S_AT

2328
15998_S_AT

18090_S_AT

2329
16028_AT

2330
16050_AT

2331
16060_S_AT

2332
16067_S_AT

2333
16072_S_AT

2334
16088_F_AT

2335
16273_AT

2336
16314_AT

2337
16413_S_AT

2338
16414_AT

2339
16426_AT

2340
16436_AT

2341
16455_AT

2342
16502_AT

2343
16548_S_AT

2344
16568_S_AT

2345
16582_S_AT

2346
16589_S_AT

2347
16594_S_AT

2348
16613_S_AT

2349
16651_S_AT

2350
16668_AT

2351
16820_AT

2352
16987_S_AT

2353
16995_AT

2354
17039_S_AT

2355
17273_AT

2356
17278_AT

2357
17433_AT

2358
17467_AT

2359
17566_AT

2360
17595_S_AT

2361
17744_S_AT

2362
17758_AT

2363
17864_AT

2364
17868_AT

2365
17876_AT

2366
17894_AT

2367
17942_S_AT

2368
18008_R_AT

2369
18027_AT

2370
18053_S_AT

2371
18062_AT

2372
18082_AT

2373
18121_S_AT

2374
18240_S_AT

2375
18248_S_AT

2376
18264_AT

2377
18276_AT

2378
18287_AT

2379
18310_AT

2380
18367_S_AT

2381
18506_AT

2382
18605_S_AT

2383
18618_S_AT

2384
18626_AT

2385
18666_S_AT

2386
18834_AT

2387
18847_AT

2388
18896_AT

2389
18899_S_AT

2390
18973_AT

2391
18983_S_AT

2392
18988_AT

2393
18998_S_AT

2394
19065_AT

2395
19119_I_AT

19121_AT

2396
19207_AT

2397
19220_AT

2398
19284_AT

2399
19315_AT

2400
19348_AT

2401
19403_S_AT

2402
19437_S_AT

2403
19502_AT

2404
19609_AT

2405
19645_AT

2406
19742_AT

2407
19863_AT

2408
19873_AT

2409
19891_AT

2410
20004_S_AT

2411
20053_AT

2412
20138_AT

2413
20193_AT

2414
20199_AT

2415
20220_AT

2416
20239_G_AT

2417
20297_AT

2418
20324_S_AT

2419
20353_AT

2420
20362_AT

2421
20389_AT

2422
20546_AT

2423
20600_AT

2424
20623_AT

2425
20629_AT

2426
20648_S_AT

2427
20668_AT

TABLE 8

2X UP IN SALT, ONLY

12037_at
14570_at
16190_at
18506_at
20648_s_at

12137_at
14578_s_at
16196_at
18605_s_at
20678_at

12153_at
14596_at
16273_at
18626_at
20686_at

12186_at
14646_s_at
16314_at
18666_s_at
20707_s_at

12216_at
14662_f_at
16413_s_at
18747_f_at

12268_at
14668_s_at
16414_at
18782_at

12449_s_at
14729_s_at
16417_s_at
18834_at

12470_at
14874_at
16455_at
18847_at

12476_at
14888_at
16548_s_at
18913_s_at

12487_at
14918_at
16582_s_at
18973_at

12493_g_at
14952_at
16589_s_at
18988_at

12609_at
14959_at
16594_s_at
18998_s_at

12685_at
14986_at
16613_s_at
19065_at

12704_f_at
15006_at
16651_s_at
19068_i_at

12709_f_at
15042_at
16668_at
19123_at

12734_f_at
15047_at
16690_g_at
19177_at

12739_s_at
15062_at
16762_at
19220_at

12750_s_at
15063_at
16820_at
19284_at

12761_s_at
15108_s_at
16873_i_at
19288_at

12819_at
15133_s_at
16987_s_at
19315_at

12845_s_at
15147_s_at
16989_at
19437_s_at

12946_at
15170_s_at
16995_at
19484_s_at

13142_at
15175_s_at
17039_s_at
19502_at

13198_i_at
15182_s_at
17040_s_at
19503_at

13229_s_at
15190_s_at
17400_s_at
19592_at

13275_f_at
15192_s_at
17425_s_at
19645_at

13344_s_at
15324_at
17433_at
19742_at

13370_at
15392_at
17467_at
19835_at

13408_s_at
15424_at
17490_s_at
19873_at

13464_at
15467_at
17529_s_at
19891_at

13472_at
15497_s_at
17543_s_at
19992_at

13526_at
15581_s_at
17566_at
20004_s_at

13614_at
15623_f_at
17595_s_at
20053_at

13652_at
15636_s_at
17744_s_at
20133_i_at

13679_s_at
15646_s_at
17758_at
20138_at

13751_at
15670_s_at
17855_at
20190_at

13918_at
15770_at
17864_at
20199_at

13919_at
15775_at
17876_at
20200_at

13944_at
15778_at
18008_r_at
20297_at

13964_at
15792_at
18013_r_at
20324_s_at

13987_s_at
15855_at
18024_s_at
20335_s_at

13993_s_at
15891_at
18027_at
20353_at

14000_at
15909_at
18053_s_at
20362_at

14032_at
15923_at
18078_at
20385_s_at

14043_at
15969_s_at
18082_at
20389_at

14052_at
15975_s_at
18090_s_at
20402_s_at

14067_at
15995_s_at
18091_at
20450_at

14070_at
15998_s_at
18121_s_at
20468_at

14269_at
16017_at
18264_at
20489_at

14285_at
16050_at
18276_at
20546_at

14427_at
16067_s_at
18300_at
20569_s_at

14501_at
16072_s_at
18367_s_at
20600_at

14540_at
16165_s_at
18471_at
20623_at

TABLE 9

2X UP SALT, 3 HR ONLY

12037_at
15042_at
16987_s_at
20004_s_at

12137_at
15047_at
16989_at
20053_at

12153_at
15062_at
17039_s_at
20133_i_at

12186_at
15063_at
17040_s_at
20138_at

12216_at
15108_s_at
17425_s_at
20190_at

12268_at
15133_s_at
17433_at
20199_at

12470_at
15147_s_at
17490_s_at
20200_at

12476_at
15170_s_at
17543_s_at
20220_at

12487_at
15175_s_at
17744_s_at
20362_at

12493_g_at
15182_s_at
17864_at
20385_s_at

12609_at
15190_s_at
17876_at
20389_at

12685_at
15192_s_at
18008_r_at
20489_at

12704_f_at
15324_at
18013_r_at
20546_at

12709_f_at
15424_at
18024_s_at
20623_at

12734_f_at
15467_at
18027_at
20648_s_at

12739_s_at
15497_s_at
18053_s_at
20678_at

12750_s_at
15623_f_at
18078_at
20707_s_at

12819_at
15636_s_at
18082_at

12946_at
15646_s_at
18090_s_at

13142_at
15670_s_at
18091_at

13229_s_at
15770_at
18121_s_at

13275_f_at
15775_at
18264_at

13370_at
15778_at
18276_at

13408_s_at
15792_at
18367_s_at

13464_at
15855_at
18471_at

13472_at
15891_at
18506_at

13614_at
15909_at
18605_s_at

13652_at
15923_at
18626_at

13679_s_at
15969_s_at
18666_s_at

13918_at
15975_s_at
18747_f_at

13919_at
15995_s_at
18782_at

13944_at
15998_s_at
18834_at

13987_s_at
16017_at
18847_at

13993_s_at
16050_at
18913_s_at

14000_at
16067_s_at
18973_at

14032_at
16072_s_at
18988_at

14043_at
16165_s_at
19065_at

14052_at
16196_at
19068_i_at

14067_at
16273_at
19123_at

14269_at
16314_at
19177_at

14285_at
16414_at
19220_at

14501_at
16417_s_at
19288_at

14540_at
16455_at
19315_at

14570_at
16548_s_at
19437_s_at

14596_at
16582_s_at
19484_s_at

14668_s_at
16589_s_at
19502_at

14729_s_at
16594_s_at
19503_at

14888_at
16613_s_at
19592_at

14918_at
16651_s_at
19645_at

14952_at
16668_at
19742_at

14959_at
16762_at
19835_at

14986_at
16820_at
19873_at

15006_at
16873_i_at
19891_at

TABLE 10

2X DOWN SALT, ONLY

12011_s_at
16046_s_at
20239_g_at

12180_at
16060_s_at
20433_at

12265_at
16088_f_at
20629_at

12335_at
16150_s_at
20668_at

12479_at
16166_s_at

12562_at
16316_at

12656_at
16340_at

12813_at
16367_i_at

13003_s_at
16426_at

13052_s_at
16427_at

13094_at
16436_at

13178_at
16489_at

13253_f_at
16502_at

13387_at
16568_s_at

13429_at
16638_s_at

13472_at
16646_s_at

13569_at
17273_at

13686_s_at
17278_at

13718_at
17567_at

13719_at
17868_at

13902_at
17880_s_at

14003_at
17894_at

14144_at
17901_at

14267_at
17942_s_at

14418_at
17960_at

14544_at
17999_at

14546_s_at
18062_at

14636_s_at
18240_s_at

14951_at
18248_s_at

14956_s_at
18267_at

14979_at
18279_s_at

14990_at
18287_at

15040_g_at
18310_at

15049_at
18351_s_at

15115_f_at
18455_at

15137_s_at
18560_at

15148_s_at
18571_at

15176_s_at
18618_s_at

15208_s_at
18896_at

15371_at
18899_s_at

15453_s_at
18967_s_at

15463_at
18983_s_at

15465_at
19119_i_at

15589_s_at
19121_at

15663_s_at
19207_at

15860_at
19348_at

15898_at
19403_s_at

15931_at
19609_at

15965_at
19742_at

15970_s_at
19826_at

15972_s_at
19863_at

16005_s_at
19883_at

16028_at
20193_at

TABLE 11

OSMOTIC STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX

ID NO:
ID NO:

2428
11994_AT

2429
12028_AT

2430
12033_AT

2431
12039_AT

2432
12068_AT

2433
12096_AT

2434
12110_AT

2435
12114_AT

2436
12135_AT

2437
12139_AT

2438
12189_AT

2439
12191_AT

2440
12211_AT

2441
12223_S_AT

2442
12366_S_AT

12869_S_AT

2443
12381_AT

2444
12406_S_AT

2445
12412_AT

2446
12453_AT

2447
12571_S_AT

2448
12662_AT

2449
12746_I_AT

2450
12774_AT

2451
12787_AT

2452
12847_AT

2453
12848_AT

2454
12895_AT

2455
12911_S_AT

2456
12920_AT

12921_S_AT

2457
13027_AT

2458
13059_AT

2459
13075_I_AT

2460
13180_S_AT

2461
13255_I_AT

2462
13270_AT

18167_S_AT

2463
13283_S_AT

2464
13382_AT

2465
13386_S_AT

2466
13433_AT

2467
13482_AT

2468
13732_AT

2469
13733_I_AT

2470
13842_AT

2471
13860_S_AT

2472
13868_AT

2473
13901_AT

2474
13933_AT

2475
13995_AT

2476
14062_AT

2477
14118_I_AT

2478
14141_AT

2479
14310_AT

2480
14354_AT

2481
14476_AT

2482
14513_S_AT

2483
14568_S_AT

2484
14604_AT

2485
14634_S_AT

2486
14660_S_AT

2487
14666_S_AT

2488
14686_S_AT

17464_AT

2489
14726_S_AT

2490
14848_S_AT

2491
14873_AT

2492
14883_AT

2493
15082_AT

2494
15121_S_AT

16014_S_AT

2495
15168_S_AT

2496
15271_AT

2497
15338_AT

2498
15418_AT

2499
15429_AT

2500
15548_AT

2501
15666_S_AT

2502
15672_S_AT

2503
15680_S_AT

2504
15867_AT

2505
15918_AT

2506
15999_S_AT

2507
16303_AT

2508
16363_AT

2509
16440_S_AT

2510
16458_S_AT

2511
16475_AT

2512
16513_S_AT

2513
16529_AT

2514
16547_S_AT

2515
16553_F_AT

2516
16563_S_AT

2517
16629_S_AT

2518
16797_AT

2519
16814_AT

2520
16832_AT

2521
16976_S_AT

2522
17007_AT

2523
17037_S_AT

2524
17054_S_AT

2525
17257_S_AT

18725_S_AT

2526
17270_AT

2527
17275_I_AT

2528
17376_AT

2529
17378_AT

2530
17468_AT

2531
17481_AT

2532
17511_S_AT

2533
17519_S_AT

2534
17815_S_AT

2535
17897_AT

2536
17923_S_AT

2537
17934_AT

2538
17937_S_AT

2539
17944_AT

2540
17958_AT

2541
18216_AT

2542
18227_AT

2543
18284_AT

2544
18301_S_AT

2545
18312_S_AT

2546
18326_S_AT

2547
18369_AT

2548
18411_AT

2549
18533_AT

2550
18576_S_AT

2551
18599_AT

2552
18640_AT

2553
18672_S_AT

2554
18720_S_AT

2555
18768_AT

2556
18877_AT

2557
18942_AT

2558
18945_AT

2559
18960_AT

2560
18965_AT

2561
19060_AT

2562
19164_G_AT

2563
19266_AT

2564
19366_S_AT

2565
19369_AT

2566
19371_AT

2567
19386_AT

2568
19412_AT

2569
19427_S_AT

2570
19622_G_AT

2571
19681_AT

2572
19819_S_AT

2573
19961_S_AT

2574
20002_AT

2575
20034_I_AT

2576
20062_AT

2577
20136_AT

2578
20223_AT

2579
20235_I_AT

2580
20401_AT

2581
20407_AT

2582
20470_AT

2583
20626_AT

2584
20631_S_AT

2585
20647_AT

TABLE 12

2X UP IN MANNITOL, ONLY

12039_at
16832_at

12068_at
16993_at

12139_at
17037_s_at

12212_at
17054_s_at

12278_at
17083_s_at

12366_s_at
17097_s_at

12453_at
17119_s_at

12556_at
17270_at

12575_s_at
17305_at

12746_i_at
17376_at

12848_at
17378_at

12869_s_at
17449_s_at

12920_at
17481_at

12921_s_at
17533_s_at

13041_s_at
17832_s_at

13059_at
17923_s_at

13241_s_at
17944_at

13255_i_at
18059_f_at

13270_at
18216_at

13382_at
18230_at

13406_at
18255_at

13433_at
18284_at

13550_at
18301_s_at

13672_s_at
18312_s_at

13716_at
18326_s_at

13842_at
18599_at

13933_at
18672_s_at

13995_at
18720_s_at

14062_at
18768_at

14075_at
18814_at

14162_at
18877_at

14208_at
18921_g_at

14217_at
18960_at

14235_at
19060_at

14310_at
19182_at

14431_at
19192_at

14513_s_at
19266_at

14584_at
19369_at

14604_at
19386_at

14673_s_at
19402_at

14856_s_at
19412_at

15207_s_at
19432_s_at

15338_at
19469_at

15406_at
19622_g_at

15418_at
19819_s_at

15591_s_at
19826_at

15666_s_at
20152_at

15680_s_at
20223_at

15866_s_at
20235_i_at

15918_at
20365_s_at

16340_at
20470_at

16553_f_at
20537_at

16797_at
20547_at

TABLE 13

2X UP IN MANNITOL, 3 HR ONLY

12039_at
17449_s_at

12068_at
17481_at

12139_at
17533_s_at

12212_at
17923_s_at

12278_at
17944_at

12366_s_at
18059_i_at

12453_at
18216_at

12556_at
18230_at

12575_s_at
18255_at

12746_i_at
18301_s_at

12848_at
18312_s_at

12869_s_at
18326_s_at

12920_at
18599_at

12921_s_at
18720_s_at

13041_s_at
18768_at

13059_at
18814_at

13241_s_at
18877_at

13382_at
18921_g_at

13406_at
18960_at

13433_at
19060_at

13550_at
19192_at

13672_s_at
19266_at

13933_at
19369_at

13995_at
19386_at

14062_at
19402_at

14075_at
19412_at

14162_at
19432_s_at

14217_at
19469_at

14310_at
19622_g_at

14431_at
19819_s_at

14513_s_at
20152_at

14584_at
20223_at

14604_at
20235_i_at

14673_s_at
20365_s_at

14856_s_at
20470_at

15207_s_at
20537_at

15338_at

15418_at

15591_s_at

15866_s_at

15918_at

16340_at

16553_f_at

16797_at

16832_at

17037_s_at

17054_s_at

17083_s_at

17097_s_at

17270_at

17305_at

17376_at

17378_at

TABLE 14

2X DOWN IN MANNITOL, ONLY

12028_at
14897_at
17958_at

12033_at
14918_at
18012_s_at

12110_at
15082_at
18227_at

12114_at
15084_at
18272_at

12189_at
15098_s_at
18331_s_at

12191_at
15105_s_at
18369_at

12211_at
15121_s_at
18411_at

12223_s_at
15126_s_at
18533_at

12268_at
15168_s_at
18576_s_at

12345_at
15271_at
18640_at

12381_at
15429_at
18696_s_at

12406_s_at
15548_at
18945_at

12412_at
15672_s_at
18949_at

12522_at
15753_at
18953_at

12571_s_at
15867_at
18965_at

12662_at
15999_s_at
19164_g_at

12787_at
16001_at
19322_at

12847_at
16021_s_at
19366_s_at

12895_at
16190_at
19371_at

12911_s_at
16260_at
19397_at

13027_at
16303_at
19427_s_at

13075_i_at
16363_at
19681_at

13221_at
16458_s_at
19707_s_at

13262_s_at
16468_at
19839_at

13283_s_at
16475_at
19961_s_at

13386_s_at
16513_s_at
19976_at

13447_s_at
16529_at
19998_at

13482_at
16563_s_at
20002_at

13634_s_at
16690_g_at
20034_i_at

13709_s_at
16814_at
20136_at

13732_at
16847_at
20382_s_at

13733_i_at
16927_s_at
20407_at

13812_s_at
16976_s_at
20529_at

13825_s_at
17007_at
20626_at

13860_s_at
17014_s_at
20631_s_at

13868_at
17016_s_at
20647_at

13901_at
17071_s_at
20699_at

14052_at
17090_s_at

14224_at
17257_s_at

14244_s_at
17275_i_at

14254_s_at
17424_at

14256_f_at
17464_at

14354_at
17468_at

14476_at
17511_s_at

14568_s_at
17519_s_at

14634_s_at
17525_s_at

14646_s_at
17645_s_at

14660_s_at
17741_at

14686_s_at
17815_s_at

14726_s_at
17897_at

14848_s_at
17899_at

14873_at
17934_at

14883_at
17937_s_at

TABLE 15

COLD & OSOMOTIC STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX

ID NO:
ID NO:

1699
12040_AT

1700
12048_AT

1701
12054_S_AT

1702
12077_AT

1703
12107_I_AT

1704
12113_AT

1705
12154_AT

1706
12171_AT

1707
12212_AT

1708
12278_AT

1709
12317_AT

1710
12325_AT

1711
12333_AT

1712
12345_AT

1713
12349_S_AT

14254_S_AT

14256_F_AT

1714
12356_AT

1715
12380_AT

1716
12392_AT

1717
12460_S_AT

1718
12556_AT

1719
12575_S_AT

1720
12686_S_AT

1721
12701_I_AT

1722
12754_G_AT

1723
12782_R_AT

1724
12784_AT

1725
12879_S_AT

1726
12891_AT

16817_S_AT

1727
12898_G_AT

1728
12974_AT

1729
12998_AT

1730
13041_S_AT

1731
13124_AT

1732
13134_S_AT

1733
13144_AT

1734
13147_AT

1735
13152_S_AT

1736
13187_I_AT

16981_S_AT

1737
13192_S_AT

17525_S_AT

1738
13212_S_AT

1739
13215_S_AT

16649_S_AT

1740
13241_S_AT

1741
13246_AT

1742
13262_S_AT

1743
13286_S_AT

1744
13324_AT

1745
13340_S_AT

1746
13361_AT

1747
13406_AT

1748
13441_S_AT

1749
13513_AT

1750
13550_AT

1751
13573_AT

1752
13577_S_AT

1753
13606_AT

1754
13609_AT

1755
13625_S_AT

1756
13626_AT

1757
13634_S_AT

1758
13672_S_AT

18916_S_AT

1759
13709_S_AT

1760
13736_AT

1761
13775_AT

1762
13810_AT

1763
13812_S_AT

1764
13825_S_AT

1765
14015_S_AT

14016_S_AT

1766
14029_AT

1767
14036_AT

1768
14051_AT

1769
14060_AT

1770
14064_AT

1771
14066_AT

1772
14075_AT

1773
14094_S_AT

19999_S_AT

1774
14096_AT

1775
14104_AT

1776
14123_S_AT

1777
14126_S_AT

1778
14131_AT

1779
14136_AT

1780
14139_AT

14140_AT

1781
14162_AT

14217_AT

1782
14178_AT

1783
14201_AT

1784
14208_AT

1785
14235_AT

1786
14242_S_AT

1787
14431_AT

1788
14480_AT

1789
14497_AT

1790
14553_AT

1791
14584_AT

1792
14600_AT

1793
14673_S_AT

19432_S_AT

1794
14681_G_AT

1795
14699_AT

1796
14751_AT

1797
14762_AT

1798
14828_S_AT

1799
14856_S_AT

1800
14882_AT

1801
14897_AT

1802
14978_AT

1803
14985_S_AT

1804
15031_AT

1805
15084_AT

1806
15096_AT

1807
15105_S_AT

1808
15110_S_AT

1809
15111_S_AT

1810
15120_S_AT

1811
15126_S_AT

1812
15142_S_AT

1813
15144_S_AT

1814
15184_S_AT

1815
15198_S_AT

1816
15203_S_AT

1817
15207_S_AT

1818
15240_AT

1819
15366_AT

1820
15398_AT

1821
15406_AT

1822
15448_AT

1823
15466_AT

1824
15481_AT

1825
15484_AT

1826
15549_AT

1827
15591_S_AT

1828
15606_S_AT

1829
15614_S_AT

16927_S_AT

1830
15629_S_AT

1831
15633_S_AT

1832
15641_S_AT

18012_S_AT

1833
15720_AT

1834
15815_S_AT

1835
15817_AT

1836
15837_AT

1837
15841_AT

1838
15866_S_AT

18255_AT

1839
15872_AT

18331_S_AT

1840
15892_AT

1841
15933_AT

1842
15947_AT

1843
15959_S_AT

1844
16001_AT

1845
16052_AT

1846
16161_S_AT

1847
16204_AT

1848
16232_S_AT

1849
16252_AT

1850
16260_AT

1851
16266_AT

1852
16299_AT

1853
16365_AT

1854
16468_AT

1855
16477_AT

1856
16491_AT

1857
16523_S_AT

1858
16566_S_AT

1859
16570_S_AT

1860
16688_AT

1861
16840_AT

1862
16847_AT

1863
16893_AT

1864
16896_S_AT

1865
16898_S_AT

1866
16912_S_AT

1867
16980_AT

1868
16993_AT

1869
17008_AT

1870
17012_S_AT

1871
17014_S_AT

1872
17016_S_AT

1873
17032_S_AT

1874
17050_S_AT

17051_S_AT

1875
17071_S_AT

1876
17090_S_AT

18690_S_AT

1877
17097_S_AT

1878
17104_S_AT

1879
17119_S_AT

1880
17160_AT

1881
17305_AT

1882
17424_AT

1883
17449_S_AT

1884
17452_G_AT

1885
17540_S_AT

1886
17552_S_AT

1887
17571_AT

1888
17589_AT

1889
17641_G_AT

1890
17741_AT

18098_AT

1891
17766_AT

1892
17873_S_AT

1893
17904_AT

1894
17920_S_AT

1895
17925_AT

1896
17943_AT

1897
18059_I_AT

1898
18230_AT

1899
18263_AT

1900
18272_AT

1901
18540_AT

1902
18608_AT

1903
18647_AT

1904
18662_S_AT

1905
18664_AT

1906
18695_S_AT

1907
18704_AT

1908
18814_AT

1909
18907_S_AT

1910
18921_G_AT

1911
18924_AT

1912
18949_AT

19707_S_AT

1913
18995_AT

1914
19017_AT

1915
19034_AT

1916
19063_AT

1917
19142_AT

1918
19158_AT

1919
19180_AT

1920
19187_AT

1921
19192_AT

1922
19195_AT

1923
19199_AT

1924
19231_AT

1925
19263_AT

1926
19308_AT

1927
19322_AT

1928
19365_S_AT

1929
19372_AT

1930
19389_AT

1931
19392_AT

1932
19397_AT

1933
19400_AT

1934
19402_AT

1935
19458_AT

1936
19469_AT

1937
19473_AT

1938
19597_S_AT

1939
19710_S_AT

1940
19830_AT

1941
19839_AT

1942
19840_S_AT

1943
19853_AT

1944
19860_AT

1945
19880_AT

1946
19889_AT

1947
19898_AT

1948
19914_AT

1949
19924_AT

1950
19949_AT

1951
19976_AT

1952
19998_AT

1953
20030_AT

1954
20151_AT

1955
20152_AT

1956
20187_AT

1957
20214_I_AT

1958
20269_AT

1959
20271_AT

1960
20273_AT

1961
20299_AT

1962
20323_AT

1963
20429_S_AT

1964
20457_AT

1965
20480_S_AT

1966
20529_AT

1967
20547_AT

1968
20555_S_AT

1969
20699_AT

TABLE 16

2X UP IN MANNITOL & COLD, ONLY

12345_at
17066_s_at

12784_at
17540_s_at

13153_r_at
17567_at

13212_s_at
17766_at

13215_s_at
17904_at

13246_at
17920_s_at

13262_s_at
17943_at

13361_at
18263_at

13625_s_at
18351_s_at

13764_at
18662_s_at

13810_at
18670_g_at

14015_s_at
18695_s_at

14016_s_at
18704_at

14060_at
18729_at

14096_at
18995_at

14123_s_at
19158_at

14139_at
19473_at

14219_at
19710_s_at

14248_at
19883_at

14254_s_at
19889_at

14256_f_at
20030_at

14609_at
20269_at

14636_s_at
20271_at

14681_g_at
20299_at

14699_at
20429_s_at

14704_s_at
20438_at

14828_s_at
20480_s_at

14882_at

15110_s_at

15184_s_at

15448_at

15629_s_at

15720_at

15846_at

15947_at

16161_s_at

16365_at

16427_at

16566_s_at

16570_s_at

16649_s_at

16688_at

16712_at

16817_s_at

16840_at

16893_at

16912_s_at

16916_s_at

16927_s_at

16981_s_at

17012_s_at

17014_s_at

17051_s_at

TABLE 17

2X DOWN COLD & MANNITOL, ONLY

12040_at
14553_at
17873_s_at

12048_at
14612_at
17925_at

12054_s_at
14751_at
18098_at

12077_at
14762_at
18540_at

12107_i_at
14978_at
18608_at

12113_at
14985_s_at
18647_at

12154_at
15031_at
18664_at

12171_at
15096_at
18690_s_at

12317_at
15111_s_at
18725_s_at

12325_at
15120_s_at
18924_at

12333_at
15142_s_at
19017_at

12356_at
15198_s_at
19034_at

12380_at
15203_s_at
19063_at

12392_at
15240_at
19141_at

12460_s_at
15366_at
19142_at

12686_s_at
15392_at
19180_at

12701_i_at
15398_at
19187_at

12782_r_at
15466_at
19195_at

12879_s_at
15481_at
19199_at

12898_g_at
15484_at
19231_at

12974_at
15549_at
19308_at

12998_at
15623_f_at
19372_at

13144_at
15815_s_at
19392_at

13147_at
15817_at
19400_at

13152_s_at
15841_at
19458_at

13192_s_at
15892_at
19597_s_at

13286_s_at
15933_at
19762_at

13324_at
15959_s_at
19830_at

13340_s_at
16052_at
19853_at

13441_s_at
16204_at
19869_at

13513_at
16252_at
19880_at

13573_at
16266_at
19898_at

13606_at
16299_at
19914_at

13609_at
16477_at
19924_at

13626_at
16491_at
19949_at

13736_at
16561_s_at
20151_at

13775_at
16645_s_at
20187_at

14029_at
16898_s_at
20214_i_at

14036_at
16980_at
20273_at

14051_at
17008_at
20323_at

14064_at
17104_s_at
20457_at

14066_at
17160_at
20555_s_at

14094_s_at
17317_at

14104_at
17400_s_at

14126_s_at
17452_g_at

14131_at
17477_s_at

14136_at
17500_s_at

14178_at
17552_s_at

14192_at
17571_at

14201_at
17572_s_at

14242_s_at
17589_at

14480_at
17641_g_at

14497_at
17855_at

TABLE 18

COLD & SALINE STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX

ID NO:
ID NO:

1970
12021_AT

1971
12037_AT

1972
12094_AT

1973
12098_AT

1974
12128_AT

1975
12148_AT

1976
12151_AT

1977
12357_S_AT

1978
12394_AT

1979
12472_S_AT

1980
12475_AT

1981
12482_S_AT

1982
12490_AT

1983
12505_S_AT

1984
12531_AT

1985
12540_S_AT

1986
12541_AT

1987
12577_AT

1988
12594_AT

1989
12629_AT

1990
12642_AT

1991
12656_AT

1992
12660_AT

1993
12712_F_AT

1994
12725_R_AT

1995
12745_AT

1996
12777_I_AT

1997
12790_S_AT

1998
12798_AT

1999
12801_AT

2000
12855_F_AT

2001
12887_S_AT

2002
12933_R_AT

2003
12951_AT

2004
13005_AT

2005
13015_S_AT

2006
13115_AT

2007
13178_AT

2008
13228_AT

2009
13236_S_AT

16646_S_AT

2010
13266_S_AT

15211_S_AT

2011
13275_F_AT

2012
13335_AT

2013
13362_S_AT

2014
13428_AT

2015
13464_AT

2016
13480_AT

2017
13538_AT

2018
13544_AT

2019
13549_AT

2020
13565_AT

2021
13580_AT

2022
13588_AT

2023
13649_AT

2024
13652_AT

2025
13679_S_AT

2026
13696_AT

2027
13702_S_AT

2028
13751_AT

2029
13919_AT

2030
13943_AT

2031
13950_S_AT

2032
14050_AT

2033
14055_S_AT

16166_S_AT

2034
14067_AT

2035
14078_AT

2036
14110_I_AT

2037
14144_AT

2038
14232_AT

2039
14285_AT

2040
14346_AT

2041
14432_AT

2042
14468_AT

2043
14479_AT

2044
14524_S_AT

2045
14608_AT

2046
14621_AT

2047
14635_S_AT

17128_S_AT

2048
14640_S_AT

2049
14643_S_AT

2050
14663_S_AT

2051
14668_S_AT

2052
14688_S_AT

18279_S_AT

2053
14737_S_AT

2054
14768_AT

2055
14875_AT

2056
14911_S_AT

17056_S_AT

2057
14924_AT

2058
14956_S_AT

15148_S_AT

18673_AT

2059
14964_AT

2060
15022_AT

2061
15040_G_AT

2062
15047_AT

2063
15063_AT

2064
15085_S_AT

2065
15123_S_AT

2066
15133_S_AT

2067
15137_S_AT

2068
15153_S_AT

2069
15170_S_AT

2070
15172_S_AT

2071
15182_S_AT

2072
15190_S_AT

2073
15241_S_AT

2074
15389_AT

2075
15453_S_AT

2076
15495_AT

2077
15496_AT

2078
15519_S_AT

2079
15562_AT

2080
15580_S_AT

2081
15582_S_AT

2082
15638_S_AT

18751_F_AT

2083
15646_S_AT

2084
15647_S_AT

2085
15654_S_AT

2086
15655_S_AT

2087
15658_S_AT

2088
15670_S_AT

2089
15775_AT

2090
15798_AT

2091
15930_AT

2092
15931_AT

2093
15949_S_AT

2094
16017_AT

2095
16053_I_AT

2096
16078_S_AT

2097
16086_S_AT

2098
16120_S_AT

2099
16126_S_AT

2100
16150_S_AT

2101
16159_S_AT

2102
16230_AT

2103
16306_AT

2104
16367_I_AT

2105
16417_S_AT

18083_R_AT

2106
16418_S_AT

2107
16423_AT

2108
16449_S_AT

2109
16484_S_AT

2110
16489_AT

2111
16565_S_AT

2112
16596_S_AT

2113
16600_S_AT

2114
16603_S_AT

2115
16638_S_AT

2116
16642_S_AT

2117
16763_AT

2118
16914_S_AT

2119
16968_AT

2120
16983_AT

2121
16989_AT

2122
17002_AT

2123
17015_S_AT

2124
17040_S_AT

18913_S_AT

2125
17232_AT

2126
17380_AT

2127
17394_S_AT

20640_S_AT

2128
17398_AT

2129
17448_AT

2130
17485_S_AT

2131
17490_S_AT

2132
17499_S_AT

2133
17505_S_AT

2134
17516_S_AT

2135
17529_S_AT

2136
17543_S_AT

2137
17593_R_AT

19858_S_AT

2138
17609_AT

2139
17698_AT

2140
17836_AT

2141
17886_AT

2142
17896_AT

2143
17901_AT

2144
17902_S_AT

2145
17913_S_AT

2146
17924_AT

2147
17954_S_AT

2148
17960_AT

2149
17991_G_AT

18967_S_AT

2150
17999_AT

2151
18057_I_AT

2152
18078_AT

2153
18091_AT

2154
18168_S_AT

2155
18252_AT

2156
18267_AT

2157
18300_AT

2158
18308_I_AT

2159
18328_AT

2160
18354_AT

2161
18402_AT

2162
18416_AT

2163
18455_AT

2164
18459_AT

2165
18571_AT

2166
18604_AT

19181_S_AT

2167
18644_AT

2168
18745_F_AT

19611_S_AT

2169
18782_AT

2170
18881_AT

2171
18904_S_AT

2172
18914_S_AT

2173
18963_AT

2174
19068_I_AT

2175
19078_AT

2176
19171_AT

2177
19177_AT

2178
19394_AT

2179
19411_AT

2180
19415_AT

2181
19466_S_AT

2182
19484_S_AT

2183
19549_S_AT

2184
19592_AT

2185
19633_AT

2186
19641_AT

2187
19669_AT

2188
19672_AT

2189
19684_AT

2190
19692_AT

2191
19746_AT

2192
19835_AT

2193
19848_S_AT

2194
19892_AT

2195
19904_AT

2196
19936_AT

2197
19974_S_AT

2198
19994_AT

2199
20005_S_AT

2200
20022_AT

2201
20032_AT

2202
20044_AT

2203
20049_AT

2204
20081_AT

2205
20133_I_AT

2206
20155_S_AT

2207
20163_S_AT

2208
20200_AT

2209
20296_S_AT

2210
20336_AT

2211
20341_AT

2212
20372_AT

2213
20385_S_AT

2214
20433_AT

2215
20489_AT

2216
20525_AT

2217
20543_AT

2218
20565_AT

2219
20570_AT

2220
20576_AT

2221
20577_AT

2222
20609_AT

2223
20646_AT

2224
20672_AT

2225
20707_S_AT

2226
20720_AT

TABLE 19

2X UP IN SALT & COLD, ONLY

12004_at

12098_at

12148_at

12251_at

12357_s_at

12394_at

12457_at

12505_s_at

12522_at

12541_at

12594_at

12606_at

12697_at

12745_at

12781_at

12798_at

12855_f_at

12945_at

12951_at

13005_at

13015_s_at

13115_at

13146_s_at

13335_at

13447_s_at

13480_at

13544_at

13549_at

13580_at

13649_at

13943_at

13950_s_at

14110_i_at

14144_at

14224_at

14432_at

14468_at

14479_at

14524_s_at

14640_s_at

14643_s_at

14735_s_at

14737_s_at

14768_at

14784_at

14924_at

15064_at

15127_s_at

15186_s_at

15189_s_at

15255_at

15389_at

15482_at

15495_at

15496_at

15519_s_at

15580_s_at

15582_s_at

15776_at

15798_at

15910_at

15931_at

15937_at

15949_s_at

15972_s_at

16048_at

16086_s_at

16120_s_at

16126_s_at

16150_s_at

16159_s_at

16230_at

16306_at

16418_s_at

16423_at

16449_s_at

16565_s_at

16603_s_at

16763_at

16968_at

16983_at

17002_at

17015_s_at

17019_s_at

17078_s_at

17232_at

17317_at

17394_s_at

17516_s_at

17585_s_at

17609_at

17698_at

17836_at

17896_at

17899_at

17902_s_at

17960_at

17963_at

18168_s_at

18252_at

18267_at

18308_i_at

18354_at

18402_at

18459_at

18484_at

18745_f_at

18904_s_at

18914_s_at

18929_s_at

18946_at

18963_at

19078_at

19137_at

19141_at

19411_at

19641_at

19672_at

19684_at

19692_at

19746_at

19762_at

19869_at

19894_at

19904_at

19936_at

19994_at

20005_s_at

20031_at

20044_at

20382_s_at

20406_g_at

20421_at

20525_at

20543_at

20565_at

20570_at

20640_s_at

20646_at

20720_at

TABLE 20

2X DOWN IN COLD & SALT, ONLY

12021_at
15123_s_at
19394_at

12094_at
15153_s_at
19415_at

12128_at
15172_s_at
19466_s_at

12151_at
15190_s_at
19549_s_at

12332_s_at
15211_s_at
19592_at

12472_s_at
15241_s_at
19633_at

12475_at
15437_at
19669_at

12482_s_at
15562_at
19848_s_at

12490_at
15638_s_at
19858_s_at

12531_at
15647_s_at
19878_at

12540_s_at
15654_s_at
19892_at

12577_at
15655_s_at
19974_s_at

12629_at
15658_s_at
20022_at

12642_at
15695_s_at
20032_at

12660_at
15846_at
20049_at

12676_s_at
15930_at
20081_at

12712_f_at
16053_i_at
20155_s_at

12725_r_at
16078_s_at
20163_s_at

12777_i_at
16229_at
20296_s_at

12790_s_at
16465_at
20336_at

12801_at
16484_s_at
20341_at

12887_s_at
16596_s_at
20365_s_at

12933_r_at
16600_s_at
20372_at

13153_r_at
16642_s_at
20489_at

13228_at
16914_s_at
20491_at

13362_s_at
17027_s_at
20576_at

13428_at
17066_s_at
20577_at

13538_at
17083_s_at
20609_at

13565_at
17128_s_at
20672_at

13588_at
17380_at

13696_at
17398_at

13702_s_at
17448_at

13716_at
17485_s_at

13764_at
17490_s_at

14050_at
17499_s_at

14055_s_at
17505_s_at

14069_at
17514_s_at

14078_at
17593_r_at

14232_at
17886_at

14346_at
17913_s_at

14608_at
17924_at

14609_at
17954_s_at

14621_at
17991_g_at

14635_s_at
18057_i_at

14663_s_at
18069_at

14688_s_at
18328_at

14691_at
18416_at

14704_s_at
18604_at

14875_at
18644_at

14911_s_at
18881_at

14964_at
19171_at

15022_at
19181_s_at

15085_s_at
19182_at

TABLE 21

OSMOTIC & SALINE STRESS RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX

ID NO:
ID NO:

2586
12126_S_AT

2587
12137_AT

2588
12227_AT

2589
12239_AT

2590
12268_AT

2591
12369_AT

2592
12476_AT

2593
12484_G_AT

2594
12494_AT

2595
12644_AT

2596
12645_AT

2597
12796_S_AT

2598
12819_AT

2599
12841_AT

2600
12852_S_AT

19455_S_AT

2601
13084_AT

2602
13171_AT

2603
13174_R_AT

2604
13596_AT

2605
13807_AT

2606
13977_AT

2607
13999_AT

2608
14052_AT

2609
14293_AT

2610
14335_AT

2611
14486_AT

2612
14506_AT

2613
14518_AT

2614
14540_AT

2615
14578_S_AT

2616
14646_S_AT

2617
14662_F_AT

15962_S_AT

2618
14901_AT

2619
14918_AT

2620
14986_AT

2621
15053_S_AT

2622
15179_S_AT

2623
15252_G_AT

2624
15280_AT

2625
15467_AT

2626
15607_S_AT

2627
15625_S_AT

2628
15703_I_AT

2629
15827_AT

2630
15863_AT

2631
15923_AT

2632
15946_S_AT

2633
16005_S_AT

2634
16073_F_AT

2635
16114_S_AT

2636
16127_S_AT

18744_F_AT

2637
16190_AT

2638
16196_AT

2639
16236_G_AT

19531_AT

2640
16310_AT

2641
16316_AT

2642
16334_S_AT

2643
16335_AT

2644
16340_AT

2645
16450_S_AT

2646
16500_AT

2647
16524_AT

2648
16533_AT

2649
16690_G_AT

2650
16762_AT

2651
16819_AT

2652
16873_I_AT

2653
16972_AT

2654
16991_AT

2655
17099_S_AT

2656
17339_AT

2657
17397_S_AT

2658
17419_AT

2659
17460_AT

2660
17554_S_AT

2661
17939_AT

2662
18013_R_AT

18178_S_AT

2663
18024_S_AT

2664
18032_I_AT

2665
18054_AT

2666
18151_AT

2667
18281_AT

2668
18445_AT

2669
18520_AT

2670
18583_AT

2671
18663_S_AT

2672
18753_S_AT

2673
18876_AT

2674
18938_G_AT

2675
18971_AT

2676
18977_AT

2677
18981_AT

2678
19099_AT

2679
19196_AT

2680
19376_AT

2681
19409_AT

2682
19503_AT

2683
19826_AT

2684
19847_S_AT

2685
19930_AT

2686
19992_AT

2687
20096_AT

2688
20108_AT

2689
20256_S_AT

2690
20290_S_AT

2691
20298_AT

2692
20305_AT

2693
20322_AT

2694
20333_AT

2695
20402_S_AT

2696
20424_AT

2697
20446_S_AT

2698
20450_AT

2699
20468_AT

2700
20569_S_AT

2701
20639_AT

2702
20678_AT

2703
20686_AT

TABLE 22

2X UP IN SALT & MANNITOL, ONLY

12126_s_at
17548_s_at

12227_at
17554_s_at

12369_at
17961_at

12521_at
18032_i_at

12644_at
18054_at

12645_at
18151_at

12724_f_at
18167_s_at

12795_at
18281_at

12796_s_at
18520_at

12841_at
18663_s_at

12852_s_at
18744_f_at

12958_at
18753_s_at

13014_at
18789_at

13174_r_at
18876_at

13211_s_at
18909_s_at

13596_at
18938_g_at

13640_at
18977_at

13789_at
19099_at

13977_at
19108_at

13999_at
19135_at

14069_at
19227_at

14083_at
19376_at

14089_at
19429_at

14293_at
19455_s_at

14675_s_at
19531_at

15053_s_at
19789_s_at

15058_s_at
19878_at

15252_g_at
20017_at

15280_at
20096_at

15437_at
20256_s_at

15607_s_at
20290_s_at

15625_s_at
20305_at

15827_at
20322_at

15863_at
20333_at

15880_at
20420_at

16005_s_at
20424_at

16031_at
20689_s_at

16073_f_at

16316_at

16334_s_at

16335_at

16450_s_at

16500_at

16524_at

16533_at

16597_s_at

16819_at

17085_s_at

17099_s_at

17339_at

17419_at

17442_i_at

17514_s_at

TABLE 23

2X DOWN IN MANNITOL & SALT, ONLY

12239_at
20108_at

12251_at
20298_at

12476_at
20421_at

12484_g_at
20432_at

12494_at
20446_s_at

12561_at
20639_at

12647_s_at

12719_f_at

12819_at

12841_at

13084_at

13171_at

13172_s_at

13435_at

13807_at

14250_r_at

14335_at

14486_at

14506_at

14518_at

14901_at

15046_s_at

15179_s_at

15451_at

15703_i_at

15946_s_at

16014_s_at

16114_s_at

16310_at

16342_at

16712_at

16762_at

16972_at

16991_at

17397_s_at

17408_at

17460_at

17775_at

17939_at

18445_at

18583_at

18751_f_at

18971_at

18981_at

19156_s_at

19196_at

19359_s_at

19409_at

19503_at

19713_at

19718_at

19847_s_at

19930_at

TABLE 24

COLD, OSMOTIC & SALINE RESPONSIVE SEQUENCES

SEQ
AFFYMETRIX

ID NO:
ID NO:

1262
12004_AT

1263
12023_S_AT

1264
12078_AT

1265
12115_AT

1266
12118_AT

1267
12150_AT

1268
12251_AT

1269
12271_S_AT

1270
12276_AT

1271
12332_S_AT

13211_S_AT

1272
12338_AT

1273
12400_AT

1274
12430_AT

1275
12457_AT

1276
12521_AT

1277
12522_AT

1278
12530_AT

1279
12536_S_AT

1280
12538_AT

1281
12561_AT

1282
12574_AT

19019_I_AT

1283
12595_AT

1284
12606_AT

1285
12609_AT

1286
12622_AT

1287
12630_AT

1288
12647_S_AT

1289
12676_S_AT

1290
12697_AT

1291
12698_AT

1292
12719_F_AT

1293
12724_F_AT

15871_S_AT

16597_S_AT

1294
12749_AT

1295
12765_AT

1296
12769_AT

1297
12781_AT

1298
12785_AT

1299
12792_S_AT

1300
12795_AT

1301
12805_S_AT

1302
12857_AT

1303
12883_S_AT

1304
12909_S_AT

16539_S_AT

1305
12932_S_AT

15605_S_AT

1306
12945_AT

1307
12958_AT

1308
12964_AT

1309
12968_AT

1310
12972_AT

1311
12989_S_AT

1312
13004_AT

1313
13014_AT

1314
13025_AT

1315
13036_AT

1316
13099_S_AT

1317
13136_AT

1318
13146_S_AT

13239_S_AT

1319
13153_R_AT

1320
13159_AT

1321
13176_AT

1322
13217_S_AT

17500_S_AT

1323
13225_S_AT

15997_S_AT

1324
13230_S_AT

15972_S_AT

1325
13279_S_AT

17477_S_AT

1326
13280_S_AT

20301_S_AT

1327
13282_S_AT

17027_S_AT

1328
13426_AT

1329
13432_AT

1330
13435_AT

1331
13447_S_AT

1332
13474_AT

1333
13511_AT

1334
13546_AT

1335
13547_S_AT

1336
13548_AT

1337
13555_AT

1338
13587_AT

1339
13595_AT

1340
13610_S_AT

1341
13627_AT

1342
13640_AT

1343
13645_AT

1344
13647_AT

1345
13706_S_AT

19701_S_AT

1346
13716_AT

18228_AT

1347
13725_AT

1348
13764_AT

1349
13771_AT

1350
13789_AT

1351
13916_AT

1352
13965_S_AT

1353
13967_AT

1354
14028_AT

1355
14039_AT

1356
14046_AT

1357
14049_AT

1358
14069_AT

1359
14077_AT

1360
14080_AT

1361
14083_AT

1362
14089_AT

1363
14090_I_AT

1364
14097_AT

1365
14116_AT

1366
14151_AT

14219_AT

1367
14170_AT

1368
14172_AT

1369
14192_AT

1370
14224_AT

1371
14227_AT

1372
14244_S_AT

14245_AT

14645_S_AT

15974_G_AT

1373
14248_AT

1374
14250_R_AT

1375
14367_AT

1376
14381_AT

1377
14384_AT

1378
14398_S_AT

1379
14487_AT

1380
14582_AT

1381
14597_AT

1382
14609_AT

1383
14612_AT

19267_S_AT

1384
14614_AT

1385
14636_S_AT

1386
14644_S_AT

14658_S_AT

14659_S_AT

15964_S_AT

1387
14675_S_AT

1388
14691_AT

14709_AT

1389
14704_S_AT

15846_AT

1390
14705_I_AT

1391
14733_S_AT

1392
14735_S_AT

1393
14779_AT

1394
14784_AT

1395
14923_AT

1396
14947_AT

1397
14950_AT

1398
14990_AT

1399
14998_AT

1400
15005_S_AT

1401
15018_AT

1402
15045_AT

1403
15046_S_AT

1404
15052_AT

1405
15058_S_AT

1406
15064_AT

1407
15088_S_AT

1408
15098_S_AT

1409
15103_S_AT

1410
15109_S_AT

1411
15124_S_AT

1412
15127_S_AT

1413
15145_S_AT

1414
15154_S_AT

1415
15161_S_AT

1416
15189_S_AT

1417
15214_S_AT

1418
15255_AT

1419
15356_AT

1420
15357_AT

1421
15364_AT

1422
15392_AT

1423
15403_S_AT

1424
15437_AT

1425
15451_AT

1426
15476_AT

1427
15482_AT

1428
15483_S_AT

1429
15521_S_AT

1430
15522_I_AT

1431
15531_I_AT

1432
15573_AT

1433
15581_S_AT

1434
15586_S_AT

1435
15594_S_AT

1436
15609_S_AT

1437
15611_S_AT

1438
15621_F_AT

1439
15623_F_AT

1440
15669_S_AT

1441
15695_S_AT

1442
15702_S_AT

1443
15753_AT

1444
15761_AT

1445
15776_AT

1446
15778_AT

1447
15839_AT

1448
15842_AT

1449
15857_S_AT

1450
15859_AT

1451
15880_AT

1452
15886_AT

1453
15906_S_AT

1454
15910_AT

1455
15937_AT

1456
15957_AT

1457
15970_S_AT

1458
15985_AT

1459
16010_S_AT

16011_S_AT

17078_S_AT

1460
16021_S_AT

1461
16031_AT

1462
16038_S_AT

1463
16045_S_AT

1464
16046_S_AT

1465
16048_AT

1466
16061_S_AT

1467
16082_S_AT

1468
16111_F_AT

1469
16115_S_AT

1470
16141_S_AT

1471
16144_S_AT

1472
16163_S_AT

1473
16173_S_AT

1474
16229_AT

1475
16298_AT

1476
16301_S_AT

1477
16322_AT

1478
16342_AT

1479
16351_AT

1480
16412_S_AT

1481
16422_AT

1482
16427_AT

1483
16438_AT

1484
16474_S_AT

1485
16482_S_AT

1486
16485_S_AT

18052_S_AT

1487
16493_AT

1488
16534_S_AT

1489
16555_S_AT

1490
16561_S_AT

17572_S_AT

1491
16592_S_AT

1492
16615_S_AT

1493
16637_S_AT

1494
16692_AT

1495
16712_AT

1496
16789_AT

1497
16818_S_AT

1498
16971_S_AT

1499
17018_S_AT

1500
17019_S_AT

1501
17029_S_AT

1502
17041_S_AT

1503
17047_S_AT

1504
17066_S_AT

1505
17085_S_AT

1506
17089_S_AT

1507
17179_AT

1508
17180_AT

1509
17228_AT

1510
17252_AT

1511
17317_AT

1512
17338_AT

1513
17384_AT

1514
17387_S_AT

1515
17400_S_AT

1516
17407_S_AT

1517
17408_AT

1518
17413_S_AT

1519
17416_AT

1520
17425_S_AT

1521
17440_I_AT

1522
17442_I_AT

1523
17473_AT

1524
17484_AT

1525
17514_S_AT

1526
17520_S_AT

1527
17533_S_AT

1528
17548_S_AT

19614_AT

1529
17549_S_AT

1530
17555_S_AT

1531
17567_AT

1532
17654_AT

1533
17693_AT

1534
17697_AT

1535
17722_AT

1536
17752_AT

1537
17755_AT

1538
17775_AT

1539
17832_S_AT

1540
17840_S_AT

1541
17843_S_AT

1542
17855_AT

1543
17860_AT

1544
17869_AT

1545
17888_AT

1546
17899_AT

1547
17929_S_AT

1548
17930_S_AT

1549
17932_S_AT

1550
17936_S_AT

18670_G_AT

1551
17957_AT

1552
17961_AT

1553
17962_AT

1554
17963_AT

1555
17971_S_AT

1556
17975_AT

18742_F_AT

1557
18016_R_AT

1558
18069_AT

1559
18122_AT

1560
18140_AT

1561
18199_AT

1562
18224_S_AT

1563
18225_AT

1564
18235_AT

1565
18259_S_AT

1566
18265_AT

1567
18270_AT1568

18280_AT

1569
18289_AT

1570
18296_AT

1571
18298_AT

1572
18314_I_AT

1573
18318_AT

1574
18325_AT

1575
18351_S_AT

1576
18471_AT

1577
18482_S_AT

1578
18484_AT

1579
18560_AT

1580
18564_AT

1581
18590_AT

1582
18594_AT

1583
18595_AT

1584
18596_AT

1585
18629_S_AT

1586
18637_AT

1587
18661_AT

1588
18668_AT

1589
18699_I_AT

1590
18747_F_AT

18789_AT

1591
18761_AT

1592
18833_AT

1593
18875_S_AT

1594
18894_AT

1595
18936_AT

1596
18946_AT

1597
18953_AT

1598
18955_AT

1599
18972_AT

1600
19008_S_AT

1601
19108_AT

1602
19123_AT

1603
19135_AT

1604
19137_AT

1605
19141_AT

1606
19152_AT

1607
19156_S_AT

1608
19182_AT

1609
19186_S_AT

1610
19214_AT

1611
19216_AT

1612
19227_AT

1613
19243_AT

1614
19288_AT

1615
19359_S_AT

1616
19368_AT

1617
19379_AT

1618
19380_S_AT

1619
19398_AT

1620
19421_AT

1621
19424_AT

1622
19429_AT

1623
19430_AT

1624
19450_AT

1625
19457_AT

1626
19467_AT

1627
19516_AT

1628
19545_AT

1629
19564_AT

1630
19577_AT

1631
19593_AT

1632
19602_AT

1633
19618_AT

1634
19638_AT

1635
19640_AT

1636
19646_S_AT

1637
19656_S_AT

1638
19670_AT

1639
19696_AT

1640
19713_AT

1641
19718_AT

1642
19722_S_AT

1643
19749_AT

1644
19755_AT

1645
19762_AT

1646
19789_S_AT

1647
19815_AT

1648
19843_AT

1649
19869_AT

1650
19878_AT

1651
19883_AT

1652
19894_AT

1653
19926_AT

1654
19944_AT

1655
19968_AT

1656
19977_AT

1657
19982_AT

1658
19987_AT

1659
19991_AT

1660
20015_AT

1661
20017_AT

1662
20031_AT

1663
20040_AT

1664
20042_S_AT

1665
20060_AT

20438_AT

1666
20089_AT

1667
20118_AT

1668
20144_AT

1669
20149_AT

1670
20179_AT

1671
20190_AT

1672
20194_AT

1673
20219_AT

1674
20245_S_AT

1675
20263_AT

1676
20308_S_AT

1677
20335_S_AT

1678
20338_AT

1679
20345_AT

1680
20365_S_AT

1681
20382_S_AT

1682
20390_S_AT

1683
20395_AT

1684
20420_AT

1685
20421_AT

1686
20432_AT

1687
20437_AT

1688
20442_I_AT

1689
20463_S_AT

1690
20491_AT

1691
20537_AT

1692
20573_AT

1693
20636_AT

1694
20638_AT

1695
20641_AT

1696
20658_S_AT

1697
20689_S_AT

1698
20698_S_AT

TABLE 25

2X UP IN COLD, SALT & MANNITOL

12023_s_at
14733_s_at
17047_s_at
19640_at

12332_s_at
14923_at
17179_at
19646_s_at

12530_at
14990_at
17180_at
19656_s_at

12536_s_at
15005_s_at
17252_at
19701_s_at

12574_at
15018_at
17384_at
19843_at

12595_at
15052_at
17407_s_at
19944_at

12698_at
15088_s_at
17484_at
19982_at

12749_at
15098_s_at
17520_s_at
19987_at

12765_at
15103_s_at
17555_s_at
19991_at

12769_at
15145_s_at
17572_s_at
20042_s_at

12785_at
15154_s_at
17722_at
20060_at

12857_at
15161_s_at
17752_at
20118_at

12964_at
15214_s_at
17840_s_at
20144_at

12972_at
15356_at
17843_s_at
20149_at

12989_s_at
15521_s_at
17860_at
20179_at

13004_at
15573_at
17929_s_at
20194_at

13025_at
15586_s_at
17936_s_at
20245_s_at

13036_at
15609_s_at
17962_at
20390_s_at

13099_s_at
15611_s_at
18052_s_at
20437_at

13136_at
15621_f_at
18069_at
20463_s_at

13176_at
15669_s_at
18122_at
20491_at

13220_s_at
15695_s_at
18199_at
20641_at

13225_s_at
15753_at
18259_s_at
20658_s_at

13230_s_at
15761_at
18280_at

13239_s_at
15857_s_at
18289_at

13426_at
15871_s_at
18314_i_at

13474_at
15964_s_at
18318_at

13548_at
15970_s_at
18325_at

13555_at
15974_g_at
18482_s_at

13595_at
15997_s_at
18590_at

13627_at
16011_s_at
18594_at

13645_at
16021_s_at
18595_at

13647_at
16038_s_at
18596_at

13706_s_at
16046_s_at
18629_s_at

13965_s_at
16082_s_at
18661_at

13967_at
16111_f_at
18668_at

14080_at
16115_s_at
18699_i_at

14090_i_at
16127_s_at
18722_s_at

14097_at
16141_s_at
18936_at

14116_at
16144_s_at
18953_at

14151_at
16163_s_at
18955_at

14172_at
16236_g_at
18972_at

14192_at
16301_s_at
19008_s_at

14244_s_at
16322_at
19152_at

14245_at
16422_at
19186_s_at

14367_at
16474_s_at
19214_at

14398_s_at
16482_s_at
19368_at

14582_at
16485_s_at
19379_at

14614_at
16555_s_at
19380_s_at

14644_s_at
16561_s_at
19421_at

14645_s_at
16592_s_at
19545_at

14658_s_at
16637_s_at
19614_at

14659_s_at
17041_s_at
19638_at

TABLE 26

2X DOWN IN COLD, MANNITOL & SALT, ONLY

12078_at
15189_s_at
17869_at
20015_at

12115_at
15357_at
17888_at
20040_at

12118_at
15364_at
17930_s_at
20089_at

12150_at
15403_s_at
17932_s_at
20190_at

12271_s_at
15476_at
17957_at
20219_at

12276_at
15483_s_at
17963_at
20263_at

12338_at
15522_i_at
17971_s_at
20301_s_at

12400_at
15531_i_at
17975_at
20308_s_at

12430_at
15594_s_at
18016_r_at
20338_at

12538_at
15702_s_at
18140_at
20345_at

12622_at
15778_at
18224_s_at
20395_at

12630_at
15839_at
18225_at
20442_i_at

12792_s_at
15842_at
18228_at
20537_at

12805_s_at
15859_at
18235_at
20573_at

12883_s_at
15872_at
18265_at
20636_at

12909_s_at
15880_at
18270_at
20638_at

12932_s_at
15886_at
18296_at
20698_s_at

12968_at
15906_s_at
18298_at

13159_at
15957_at
18471_at

13217_s_at
15985_at
18564_at

13279_s_at
16045_s_at
18637_at

13282_s_at
16061_s_at
18742_f_at

13432_at
16173_s_at
18761_at

13511_at
16298_at
18833_at

13546_at
16351_at
18875_s_at

13547_s_at
16412_s_at
18894_at

13587_at
16438_at
18946_at

13610_s_at
16493_at
19123_at

13640_at
16534_s_at
19216_at

13725_at
16539_s_at
19243_at

13771_at
16615_s_at
19267_s_at

13916_at
16692_at
19288_at

14028_at
16789_at
19398_at

14039_at
16818_s_at
19424_at

14046_at
16971_s_at
19430_at

14049_at
17018_s_at
19450_at

14077_at
17029_s_at
19457_at

14170_at
17089_s_at
19467_at

14227_at
17228_at
19516_at

14248_at
17338_at
19564_at

14381_at
17387_s_at
19577_at

14384_at
17413_s_at
19593_at

14487_at
17416_at
19602_at

14597_at
17425_s_at
19618_at

14705_i_at
17440_i_at
19670_at

14709_at
17473_at
19696_at

14779_at
17533_s_at
19722_s_at

14947_at
17549_s_at
19749_at

14950_at
17654_at
19755_at

14998_at
17693_at
19815_at

15045_at
17697_at
19926_at

15109_s_at
17755_at
19968_at

15124_s_at
17832_s_at
19977_at

TABLE 27

2X ROOT SPECIFIC (COLD, SALINE

& OSMOTIC STRESSES)

11997_at
14069_at
16052_at
18327_s_at

12004_at
14072_at
16053_i_at
18597_at

12051_at
14073_at
16105_s_at
18607_s_at

12072_at
14097_at
16161_s_at
18636_at

12150_at
14139_at
16165_s_at
18663_s_at

12151_at
14235_at
16298_at
18782_at

12166_i_at
14250_r_at
16334_s_at
18885_at

12219_at
14578_s_at
16422_at
18888_at

12315_at
14582_at
16427_at
18942_at

12332_s_at
14640_s_at
16440_s_at
18955_at

12374_i_at
14643_s_at
16442_s_at
19060_at

12482_s_at
14644_s_at
16468_at
19108_at

12515_at
14658_s_at
16488_at
19135_at

12522_at
14659_s_at
16511_at
19137_at

12538_at
14711_s_at
16529_at
19195_at

12571_s_at
14900_at
16553_f_at
19263_at

12574_at
14924_at
16568_s_at
19376_at

12609_at
14990_at
16914_s_at
19406_at

12678_i_at
15018_at
16965_s_at
19432_s_at

12698_at
15022_at
16981_s_at
19835_at

12749_at
15107_s_at
16989_at
19836_at

12760_g_at
15116_f_at
17033_s_at
19840_s_at

12765_at
15120_s_at
17066_s_at
19841_at

12768_at
15124_s_at
17085_s_at
19843_at

12769_at
15131_s_at
17252_at
19926_at

12772_at
15132_s_at
17376_at
19972_at

12777_i_at
15137_s_at
17378_at
19977_at

12958_at
15184_s_at
17388_at
19991_at

12989_s_at
15188_s_at
17415_at
20034_i_at

13015_s_at
15208_s_at
17429_s_at
20042_s_at

13134_s_at
15252_g_at
17463_at
20189_at

13146_s_at
15343_at
17485_s_at
20194_at

13172_s_at
15389_at
17490_s_at
20200_at

13178_at
15392_at
17567_at
20214_i_at

13179_at
15448_at
17585_s_at
20239_g_at

13187_i_at
15503_at
17595_s_at
20262_at

13211_s_at
15531_i_at
17840_s_at
20269_at

13239_s_at
15594_s_at
17860_at
20294_at

13273_s_at
15609_s_at
17880_s_at
20312_s_at

13297_s_at
15623_f_at
17894_at
20382_s_at

13549_at
15639_s_at
17896_at
20396_at

13604_at
15670_s_at
17899_at
20432_at

13629_s_at
15680_s_at
17911_at
20444_at

13706_s_at
15859_at
17935_at
20446_s_at

13714_at
15900_at
17961_at
20480_s_at

13751_at
15923_at
18024_s_at
20586_i_at

13895_at
15962_s_at
18122_at
20612_s_at

13933_at
15964_s_at
18222_at
20672_at

13967_at
15965_at
18224_s_at
20686_at

13985_s_at
15975_s_at
18252_at
20689_s_at

14028_at
15985_at
18255_at

14030_at
16001_at
18269_s_at

14058_at
16048_at
18270_at

TABLE 28

2X LEAF SPECIFIC (COLD, SALINE

& OSMOTIC STRESSES)

12169_i_at
16136_s_at

12186_at
16172_s_at

12187_at
16316_at

12211_at
16385_s_at

12212_at
16455_at

12214_g_at
16485_s_at

12270_at
16512_s_at

12645_at
16547_s_at

12754_g_at
16548_s_at

12774_at
16629_s_at

12793_at
16673_at

12796_s_at
16899_at

12910_s_at
17010_s_at

12916_s_at
17018_s_at

12953_at
17054_s_at

13090_at
17095_s_at

13124_at
17097_s_at

13335_at
17273_at

13550_at
17394_s_at

13567_at
17420_at

13568_at
17449_s_at

13596_at
17600_s_at

13614_at
17843_s_at

13678_s_at
17913_s_at

13719_at
17966_at

14014_at
18003_at

14096_at
18081_at

14118_i_at
18560_at

14369_at
18588_at

14478_at
18626_at

14513_s_at
18644_at

14540_at
18666_s_at

14596_at
18742_f_at

14733_s_at
18977_at

14986_at
18994_at

15045_at
19227_at

15097_s_at
19373_at

15098_s_at
19834_at

15145_s_at
19867_at

15153_s_at
19998_at

15154_s_at
20062_at

15182_s_at
20199_at

15203_s_at
20256_s_at

15372_at
20284_at

15521_s_at
20437_at

15581_s_at
20442_i_at

15621_f_at
20450_at

15642_s_at
20468_at

15776_at
20547_at

15910_at
20635_s_at

16017_at

16046_s_at

16115_s_at

TABLE 29

2X TRANSCRIPTION (COLD, SALINE

& OSMOTIC STRESSES)

12068_at
15665_s_at
19836_at

12166_i_at
15679_s_at
19860_at

12374_i_at
15720_at
19866_at

12392_at
15871_s_at
19898_at

12431_at
16072_s_at
20262_at

12450_s_at
16073_f_at
20335_s_at

12503_at
16105_s_at
20362_at

12536_s_at
16111_f_at
20424_at

12540_s_at
16127_s_at
20437_at

12541_at
16534_s_at
20456_at

12587_at
16582_s_at
20515_s_at

12594_at
16589_s_at
20635_s_at

12595_at
16747_at

12704_f_at
17019_s_at

12705_f_at
17129_s_at

12709_f_at
17160_at

12712_f_at
17520_s_at

12719_f_at
17538_s_at

12724_f_at
17555_s_at

12725_r_at
17609_at

12726_f_at
17896_at

12734_f_at
17971_s_at

12736_f_at
17975_at

12737_f_at
17978_s_at

12812_at
18121_s_at

12949_at
18167_s_at

12951_at
18197_at

12966_s_at
18222_at

13023_at
18318_at

13034_s_at
18576_s_at

13087_at
18629_s_at

13270_at
18738_f_at

13273_s_at
18742_f_at

13432_at
18744_f_at

13555_at
18745_f_at

13688_s_at
18747_f_at

13714_at
18750_f_at

13965_s_at
18751_f_at

13987_s_at
18789_at

14003_at
18834_at

14144_at
18942_at

14178_at
19083_at

14223_at
19202_at

14235_at
19209_s_at

14303_s_at
19232_s_at

14393_at
19315_at

14553_at
19489_s_at

14781_at
19611_s_at

15046_s_at
19646_s_at

15053_s_at
19707_s_at

15214_s_at
19722_s_at

15510_r_at
19744_at

15638_s_at
19755_at

TABLE 30

2X PHOSPHATES (COLD, SALINE & OSMOTIC STRESSES)

12470_at

12556_at

13128_at

13135_s_at

13180_s_at

13192_s_at

13193_s_at

13587_at

13995_at

14335_at

15073_at

15171_s_at

15240_at

15586_s_at

15641_s_at

15651_f_at

15990_at

16232_s_at

16576_f_at

16753_at

17423_s_at

17525_s_at

17537_s_at

17929_s_at

17954_s_at

18012_s_at

18308_i_at

18616_at

18847_at

18936_at

18980_at

19243_at

19263_at

19638_at

19883_at

19932_at

20333_at

20393_at

20570_at

TABLE 31

2X KINASES (COLD, SALINE & OSMOTIC STRESSES)

12253_g_at
16059_s_at
20144_at

12270_at
16087_s_at
20219_at

12271_s_at
16088_f_at
20223_at

12276_at
16125_s_at
20232_s_at

12278_at
16137_s_at
20235_i_at

12284_at
16140_s_at
20282_s_at

12300_at
16143_s_at
20298_at

12307_at
16144_s_at
20396_at

12353_at
16160_f_at
20439_at

12357_s_at
16171_s_at
20462_at

12390_at
16357_at

12394_at
16412_s_at

12395_s_at
16568_s_at

12408_at
16570_s_at

12452_at
16571_s_at

12477_at
16584_s_at

12490_at
16651_s_at

12497_at
16652_s_at

12532_at
16672_at

12697_at
16818_s_at

12901_s_at
16840_at

12902_at
17068_s_at

12958_at
17122_s_at

12959_at
17252_at

13068_at
17323_at

13246_at
17475_at

13324_at
17752_at

13332_at
17921_s_at

13362_s_at
17933_at

13370_at
17935_at

13550_at
18013_r_at

14030_at
18046_s_at

14048_at
18122_at

14194_at
18176_at

14196_at
18316_at

14217_at
18455_at

14459_at
18459_at

14603_at
18482_s_at

14637_s_at
18543_at

14686_s_at
18706_s_at

15005_s_at
18782_at

15175_s_at
18924_at

15270_at
19117_s_at

15475_s_at
19437_s_at

15497_s_at
19442_at

15577_s_at
19458_at

15616_s_at
19464_at

15633_s_at
19469_at

15634_s_at
19562_at

15668_s_at
19655_at

15680_s_at
19749_at

15798_at
19854_at

16034_at
19904_at

Number	Date	Country
1 033 405	Sep 2000	EP
WO 0008187	Feb 2000	WO

	Number	Date	Country
	20020160378 A1	Oct 2002	US
	20040009476 A9	Jan 2004	US

Number	Date	Country
60300111	Jun 2001	US
60264647	Jan 2001	US
60227866	Aug 2000	US

Stress-regulated genes of plants, transgenic plants containing same, and methods of use

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Date Issued

Inventors

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Examiners

Agents

CPC

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Foreign Referenced Citations (2)

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