SUBSTANCE INTRODUCTION UNIT AND SUBSTANCE INTRODUCTION DEVICE

Abstract

A substance introduction unit, used for introduction of a substance into a cell by electroporation, includes an accommodation container configured to accommodate a cell suspension containing the cell and the substance; and a pair of electrodes which has electrode surfaces exposed to an internal space of the accommodation container and is configured to apply a voltage to the cell suspension accommodated in the accommodation container, the accommodation container including a cell-impermeable member configured to partition the internal space of the accommodation container.

Description

Claims

1. A substance introduction unit, which is used for introduction of a substance into a cell by electroporation, comprising: an accommodation container configured to accommodate a cell suspension containing the cell and the substance; anda pair of electrodes having electrode surfaces exposed to an internal space of the accommodation container and configured to apply a voltage to the cell suspension accommodated in the accommodation container,wherein the accommodation container includes a cell-impermeable member configured to partition the internal space of the accommodation container.

2. The substance introduction unit according to claim 1, wherein the cell-impermeable member is located on an inner side of the electrode surfaces exposed to the internal space of the accommodation container.

3. The substance introduction unit according to claim 1, wherein the cell-impermeable member is a porous membrane having cell impermeability.

4. The substance introduction unit according to claim 1, wherein the cell-impermeable member is configured to partition the internal space of the accommodation container into an internal space where the electrode surfaces of the pair of electrodes are exposed and another internal space.

5. The substance introduction unit according to claim 4, wherein the cell-impermeable member has a cylindrical shape extending in a longitudinal direction of the accommodation container.

6. The substance introduction unit according to claim 1, wherein the cell-impermeable member is configured to partition the internal space of the accommodation container into a first internal space where an electrode surface of an anode out of the pair of electrodes is exposed and into a second internal space.

7. The substance introduction unit according to claim 1, wherein the cell-impermeable member is arranged in the accommodation container out of contact with the electrode surfaces.

8. A substance introduction device, which introduces a substance into a cell by electroporation, comprising the substance introduction unit according to claim 1.