Claims
- 1. An isolated LMP nucleic acid comprising a polynucleotide sequence selected from the group consisting of:
a) a polynucleotide of SEQ ID NO:2; b) a polynucleotide of SEQ ID NO:5; c) a polynucleotide of SEQ ID NO:8; d) a polynucleotide of SEQ ID NO:11; e) a polynucleotide of SEQ ID NO:14; f) a polynucleotide of SEQ ID NO:17; g) a polynucleotide of SEQ ID NO:20; h) a polynucleotide of SEQ ID NO:23; i) a polynucleotide of SEQ ID NO:26; j) a polynucleotide of SEQ ID NO:29; k) a polynucleotide of SEQ ID NO:32; l) a polynucleotide of SEQ ID NO:34; m) a polynucleotide of SEQ ID NO:37; and n) a polynucleotide of SEQ ID NO:40.
- 2. An isolated LMP nucleic acid comprising a polynucleotide sequence selected from the group consisting of:
a) a polynucleotide encoding a polypeptide of SEQ ID NO:3; b) a polynucleotide encoding a polypeptide of SEQ ID NO:6; c) a polynucleotide encoding a polypeptide of SEQ ID NO:9; d) a polynucleotide encoding a polypeptide of SEQ ID NO:12; e) a polynucleotide encoding a polypeptide of SEQ ID NO:15; f) a polynucleotide encoding a polypeptide of SEQ ID NO:18; g) a polynucleotide encoding a polypeptide of SEQ ID NO:21; h) a polynucleotide encoding a polypeptide of SEQ ID NO:24; i) a polynucleotide encoding a polypeptide of SEQ ID NO:27; j) a polynucleotide encoding a polypeptide of SEQ ID NO:30; k) a polynucleotide encoding a polypeptide of SEQ ID NO:33; l) a polynucleotide encoding a polypeptide of SEQ ID NO:36; m) a polynucleotide encoding a polypeptide of SEQ ID NO:39; and n) a polynucleotide encoding a polypeptide of SEQ ID NO:42.
- 3. An isolated nucleic acid comprising a polynucleotide of least 60 consecutive nucleotides the LMP nucleic acid of claim 1 or claim 2.
- 4. An isolated nucleic acid comprising a polynucleotide having at least 70% sequence identity with the LMP nucleic acid of claim 1 or claim 2.
- 5. An isolated nucleic acid comprising a polynucleotide having at least 90% sequence identity with the LMP nucleic acid of claim 1 or claim 2.
- 6. An isolated nucleic acid comprising a polynucleotide complementary to the LMP nucleic acid of claim 1 or claim 2.
- 7. The isolated LMP nucleic acid of claim 1, wherein the nucleic acid encodes a polypeptide that functions as a modulator of a seed storage compound in a plant.
- 8. The isolated LMP nucleic acid of claim 7, wherein the nucleic acid encodes a polypeptide that contains a transcription factor domain.
- 9. The isolated LMP nucleic acid of claim 8, wherein the nucleic acid encodes a polypeptide of SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:15 or SEQ ID NO:36.
- 10. The isolated LMP nucleic acid of claim 7, wherein the nucleic acid encodes a polypeptide that contains a protein kinase domain.
- 11. The isolated LMP nucleic acid of claim 10, wherein the nucleic acid encodes a polypeptide of SEQ ID NO:12, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:30 or SEQ ID NO:42.
- 12. The isolated LMP nucleic acid of claim 7, wherein the nucleic acid encodes a polypeptide that contains a signal transduction domain.
- 13. The isolated LMP nucleic acid of claim 12, wherein the nucleic acid encodes a polypeptide of SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:33 or SEQ ID NO:39.
- 14. A first isolated nucleic acid that hybridizes under stringent conditions to a second nucleic acid selected from the group consisting of:
a) a second nucleic acid comprising a polynucleotide of SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:10, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:19, SEQ ID NO:22, SEQ ID NO:25, SEQ ID NO:28, SEQ ID NO:31, SEQ ID NO:34, SEQ ID NO:37 or SEQ ID NO:40; and b) a second nucleic acid encoding a polypeptide of SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:15, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:24, SEQ ID NO:27, SEQ ID NO:30, SEQ ID NO:33, SEQ ID NO:36, SEQ ID NO:39 or SEQ ID NO:42; wherein the first nucleic acid encodes a polypeptide that functions as a modulator of a seed storage compound in a plant.
- 15. A recombinant expression vector comprising the LMP nucleic acid of claim 1, wherein expression of the vector in a host cell modifies a level of a seed storage compound in the host cell.
- 16. A transgenic plant cell comprising the LMP nucleic acid of claim 1.
- 17. The transgenic plant cell of claim 16, wherein expression of the LMP nucleic acid in the plant cell results in a modified level of a seed storage compound in the plant cell as compared to a wild type variety of the plant cell.
- 18. A transgenic plant comprising the LMR nucleic acid of claim 1.
- 19. The transgenic plant of claim 18, wherein the plant is a dicotyledonous plant.
- 20. The transgenic plant of claim 18, wherein the plant is a monocotyledonous plant.
- 21. The transgenic plant of claim 18, wherein the plant is an oil producing species.
- 22. The transgenic plant of claim 18, wherein the plant is selected from the group consisting of rapeseed, canola, linseed, soybean, sunflower, maize, oat, rye, barley, wheat, sugarbeet, tagetes, cotton, oil palm, coconut palm, flax, castor and peanut.
- 23. The transgenic plant of claim 18, wherein expression of the LMR nucleic acid in the plant results in a modified level of a seed storage compound in the plant as compared to a wild type variety of the plant.
- 24. The transgenic plant of claim 18, wherein the level of the seed storage compound is increased.
- 25. The transgenic plant of claim 18, wherein the seed storage compound is selected from the group consisting of a lipid, a fatty acid, a starch and a seed storage protein.
- 26. A seed produced by the transgenic plant of claim 18, wherein the plant is true breeding for a modified level of the seed storage compound as compared to a wild type variety of the plant.
- 27. A seed oil produced by the seed of claim 26.
- 28. A method of producing a transgenic plant having a modified level of a seed storage compound comprising, transforming a plant cell with an expression vector comprising a LMP nucleic acid and generating from the plant cell the transgenic plant, wherein the LMP nucleic acid comprises a polynucleotide sequence selected from the group consisting of:
a) a polynucleotide of SEQ ID NO:2; b) a polynucleotide of SEQ ID NO:5; c) a polynucleotide of SEQ ID NO:8; d) a polynucleotide of SEQ ID NO:11; e) a polynucleotide of SEQ ID NO:14; f) a polynucleotide of SEQ ID NO:17; g) a polynucleotide of SEQ ID NO:20; h) a polynucleotide of SEQ ID NO:23; i) a polynucleotide of SEQ ID NO:26; j) a polynucleotide of SEQ ID NO:29; k) a polynucleotide of SEQ ID NO:32; l) a polynucleotide of SEQ ID NO:34; m) a polynucleotide of SEQ ID NO:37; and n) a polynucleotide of SEQ ID NO:40.
- 29. A method of producing a transgenic plant having a modified level of a seed storage compound comprising, transforming a plant cell with an expression vector comprising a LMP nucleic acid and generating from the plant cell the transgenic plant, wherein the LMP nucleic acid comprises a polynucleotide sequence selected from the group consisting of:
a) a polynucleotide encoding a polypeptide of SEQ ID NO:3; b) a polynucleotide encoding a polypeptide of SEQ ID NO:6; c) a polynucleotide encoding a polypeptide of SEQ ID NO:9; d) a polynucleotide encoding a polypeptide of SEQ ID NO:12; e) a polynucleotide encoding a polypeptide of SEQ ID NO:15; f) a polynucleotide encoding a polypeptide of SEQ ID NO:18; g) a polynucleotide encoding a polypeptide of SEQ ID NO:21; h) a polynucleotide encoding a polypeptide of SEQ ID NO:24; i) a polynucleotide encoding a polypeptide of SEQ ID NO:27; j) a polynucleotide encoding a polypeptide of SEQ ID NO:30; k) a polynucleotide encoding a polypeptide of SEQ ID NO:33; l) a polynucleotide encoding a polypeptide of SEQ ID NO:36; m) a polynucleotide encoding a polypeptide of SEQ ID NO:39; and n) a polynucleotide encoding a polypeptide of SEQ ID NO:42.
- 30. The method of claim 28 or claim 29, wherein the plant is a dicotyledonous plant.
- 31. The method of claim 28 or claim 29, wherein the plant is a monocotyledonous plant.
- 32. The method of claim 28 or claim 29, wherein the plant is an oil producing species.
- 33. The method of claim 28 or claim 29, wherein the plant is selected from the group consisting of rapeseed, canola, linseed, soybean, sunflower, maize, oat, rye, barley, wheat, sugarbeet, tagetes, cotton, oil palm, coconut palm, flax, castor and peanut.
- 34. The method of claim 28 or claim 29, wherein the level of the seed storage compound is increased.
- 35. The method of claim 28 or claim 29, wherein the seed storage compound is selected from the group consisting of a lipid, a fatty acid, a starch and a seed storage protein.
- 36. A method of modulating a level of a seed storage compound in a plant comprising, modifying the expression of a LMP nucleic acid in the plant, wherein the LMP nucleic acid is selected from the group consisting of:
a) a polynucleotide of SEQ ID NO:2; b) a polynucleotide of SEQ ID NO:5; c) a polynucleotide of SEQ ID NO:8; d) a polynucleotide of SEQ ID NO:11; e) a polynucleotide of SEQ ID NO:14; f) a polynucleotide of SEQ ID NO:17; g) a polynucleotide of SEQ ID NO:20; h) a polynucleotide of SEQ ID NO:23; i) a polynucleotide of SEQ ID NO:26; j) a polynucleotide of SEQ ID NO:29; k) a polynucleotide of SEQ ID NO:32; l) a polynucleotide of SEQ ID NO:34; m) a polynucleotide of SEQ ID NO:37; and n) a polynucleotide of SEQ ID NO:40.
- 37. A method of modulating a level of a seed storage compound in a plant comprising, modifying the expression of a LMP nucleic acid in the plant, wherein the LMP nucleic acid is selected from the group consisting of:
a) a polynucleotide encoding a polypeptide of SEQ ID NO:3; b) a polynucleotide encoding a polypeptide of SEQ ID NO:6; c) a polynucleotide encoding a polypeptide of SEQ ID NO:9; d) a polynucleotide encoding a polypeptide of SEQ ID NO:12; e) a polynucleotide encoding a polypeptide of SEQ ID NO:15; f) a polynucleotide encoding a polypeptide of SEQ ID NO:18; g) a polynucleotide encoding a polypeptide of SEQ ID NO:21; h) a polynucleotide encoding a polypeptide of SEQ ID NO:24; i) a polynucleotide encoding a polypeptide of SEQ ID NO:27; j) a polynucleotide encoding a polypeptide of SEQ ID NO:30; k) a polynucleotide encoding a polypeptide of SEQ ID NO:33; l) a polynucleotide encoding a polypeptide of SEQ ID NO:36; m) a polynucleotide encoding a polypeptide of SEQ ID NO:39; and n) a polynucleotide encoding a polypeptide of SEQ ID NO:42.
- 38. The method of claim 36 or claim 37, wherein the plant is a dicotyledonous plant.
- 39. The method of claim 36 or claim 37, wherein the plant is a monocotyledonous plant.
- 40. The method of claim 36 or claim 37, wherein the plant is an oil producing species.
- 41. The method of claim 36 or claim 37, wherein the plant is selected from the group consisting of rapeseed, canola, linseed, soybean, sunflower, maize, oat, rye, barley, wheat, sugarbeet, tagetes, cotton, oil palm, coconut palm, flax, castor and peanut.
- 42. The method of claim 36 or claim 37, wherein the level of the seed storage compound is increased.
- 43. The method of claim 36 or claim 37, wherein the seed storage compound is selected from the group consisting of a lipid, a fatty acid, a starch and a seed storage protein.
- 44. The method of claim 36 or claim 37, wherein the plant is transgenic.
- 45. The method of claim 36 or claim 37, wherein the plant is not transgenic.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] The present invention claims the priority benefit of U.S. Provisional Patent Application Serial No. 60/276,993 filed Mar. 16, 2001.
Provisional Applications (1)
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Number |
Date |
Country |
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60276993 |
Mar 2001 |
US |