Patent Application
20150315135
This invention is related to the Sulphate chelating agents that have the capability to enlarge the membrane porosity of simple microorganism cell and its synthesis process.
Many types of chelating agents are commonly known and have been applied in various industry. Chelating agent are chemicals contain donor atoms that have the capability to bind metallic atoms in coordinative complex bound that turn into cyclic structural subtances known as chelating complex or in its simple term a chelat. The chelation technology was developed from some naturally occurred chemicals that contain naturally or purposely added metallic ions. The use of chelating agent might give a way to control or manipulate the metallic ions in the system to perform the expected effect. The chelating complex subtances formed from the interaction of some metallic ion with some chelating agent use to have significantly different characteristics/properties either from its original ions or the chelating agent itself. Therefore its characteristics or properties could be modified.
Therefore, the chelating agents are the very effective subtances in the formation of the complex subtances with the metallic cations and also with the organic salts in order to prevent them act as simple hydrated cations. Common example of chelating agent is Ethylene Di Amine Tetra Acetic acid (EDTA) (1) and its derivatives that will form the complex subtances with most of M2+ and M3+ types of cations.
It has been known also that gluconic acid and some others hydroxide acid perform similar properties.
There are many features of the chelating reaction that become the basic of chelating agents various applications.
The first, chelation provide a mechanism to control the concentration of the free metallic ions via dissociation equilibrium between chelating agent and the said metallic ions. The related application as the sequestration process that reduces several properties of some metal without eliminate it from the existing system or phase, solubilization is a process that elaborate undissolved component phase become soluble in the said medium, and daparing, a condition where the addition or removal of several metallic ions result insignificance change of the said ionic concentration inside the solution which totally depending on the accurate control of the chelating agent concentration.
All of the above features and properties have been chemically applied in industry.
For example, the sequestration of the metallic ions could be used to control the water hardness. Then solubilization is used to dissolve the boiler scale, heat exchange equipment scale and the hard scale in the pipe.
In the minning industry, solubilization utilizes chelating agents to extract the metal from the metal ores, also to be used to clean the contaminated area.
Daparing with the chelating agents find its use to supply the metallic ions micro nutrient for the biological growth at very low stabilized concentration.
Secondly, on some applications as the chelating ligand catalyst which really has the catalytic activity dan has been applied as catalyst on the unsymmetrical pharmaceutical subtances synthesis.
Finally, chelating agents have also been used for human medication. For example, the removal/cleaning with the chelating agent with its chelation termonation, including the curing on the lead (Pb) poisoning and other metals with EDTA (Ethylene Di Amine Tetra Acetic acid) where lead almostly chelated to EDTA, therefore could be removed from the system.
In the publication by H. HAQUE AND A. D. RUSSELL “Effect of Chelating Agents on the Susceptibility of Some Strains of Gram-Negative Bacteria to Some Antibacterial Agents”, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, August 1974, page. 200-206, explain that some chelating agents improve the activity of beta-lactam in the bacterial inhibition of P. aeruginosa strain through the metallic removal from the nutrient medium and some chelating agent could increase the bacterial sensitivity against some antibiotics.
In the other journal published by Kaur P, Vadehra D V., “Effect of certain chelating agents on the antibacterial action of silver nitrate.” J Hyg Epidemiol Microbiol Immunol. 1988; 32(3):299-306, explain that EDTA and EGTA when applied together with AgNO3 significantly increase the antibacterial action against Staphylococcus aureus, where EDTA and EGTA also increase the sensitivity of Staphylococcus aureus bacteria that is resistant against AgNO3.
In the publication by A. Hinton Jr. and K. D. Ingram, “Comparison of the Antibacterial Activity of Chelating Agents Using the Agar Diffusion Method” International Journal of Poultry Science 9 (11): 1023-1026, 2010 explain that the addition of EDTA chelating agent for the cleaning agent formulation in the poultry treatment could improve the cleaning capability that has the antimicrobial activity helping to reduce poultry corpses contamination.
But until now, there is no publication describing that EDTA itself alone can perform as an antibacterial agent, due to the fact that chelating agents in some cases have the ability to influence simple cell membranes or other organic membranes.
In the US 2003/0055007 patent publication, Al was disclosing about some lignin sulphonate substance that has the antiviral activity against HIV and also act as an antibacterial agent, but there was no further explanation about the inhibition mechanism of the said subtances as the antiviral and antibacterial agent.
Therefore, it needs the development of new chelating agents and its derivatives that are non toxic or less toxic and induce no negative or less negative effect into environment, these features will significantly increase the application of the said chelating agents.
Unexpectedly, the inventor has discover such a new chelating agent that act alone as antibacterial agent. The inventor has invent a series of sulphate chelating agents that have the antibacterial activity by enlarging the cell membranes porosity of simple cells and the other organic membranes. The membrane porosity enlargement mechanism for simple cell or other organic membranes occurred due to the reaction of the sulphate groups of some sulphate chelating agent with the hydroxyl group (OH−) or ammine group (NH2−) in the peptide bound of modular bound structural of a protein or modular bound structural of a peptidoglycan and their similarities as the main ingredient of the simple cell wall membranes or other organic membranes.
Moreover, these invented subtances have the capability as common chelating agent or subtances that capable to open/enlarge the membrane porosity of either simple cell membrane wall or another organic membranes, also have the potential biocidal property, and it has been discovered that the said subtances have a very low toxicity against mammal and also easy to be degraded in the environment. All these features put the said subtances become very useful in many applications.
In one manifestation the present invention provides all the subtances have the structure as of formula I
wherein:
X1, X2, X3, are selected from. Hydrogen, hydroxy, halide, sulphite, sulphate, sulphonate, phosphorus derivatives, nitrogen derivatives, where X1, X2, X3, could be similar or different atoms, molecules or groups.
Rx, Rz are selected from: Hydrogen, hydroxy, halide, alkyl n, alkylen C1-20, alkyl alcohol C1-20, aliphatic or branched substituted or non substituted, aryl, cycloalkyl substituted or non substituted, sulphite, sulphate, sulphonate, phosphorus derivatives, nitrogen derivatives.
M: are Hydrogen, or group I, group II, transition group cation that are pharmaceutically acceptable.
n: intreger from 0-3
a: intreger
and/or its isomer, enantiomer, stereotiomer, salt, solvate, hydrate
In other preferred embodiment, this invention provide some subtances selected from:
In the further embodiment, the preferred subtances is where M is Natrium.
In the further embodiment, including its isomer, enantiomer, stereotiomer, salt, solvate, hydrate of Formula I.
In the further embodiment, the preferred subtances are:
In such invention embodiment provides some sulphate chelating agent represent by the above Formula I that has antibacterial activity by enlarging the membrane porosity of simple cells or another organic membranes. The membrane porosity enlargement mechanism for simple cell or other organic membranes occurred due to the reaction of the sulphate groups of some sulphate chelating agent with the hydroxyl group (OH−) or ammine group (NH2−) in the peptide bound of modular bound structural of a protein or modular bound structural of a peptidoglycan and their similarities as the main ingredient of the simple cell wall membranes or other organic membranes.
In another embodiment, this invention provide some series sulphate chelating agents represent by the above Formula I that can be used as biocide.
Further embodiment of this invention is providing the synthesis process of the said sulphate chelating agent represent by Formula I, that consist of:
X1, X2, X3, Rx, Rz, and a as have already defined in the above Formula I;
With sulphuric acid (H2SO4) or sulphonic acid (H2SO3) and/or sulfur triokside (SO3) or sulfur diokside (SO2) as it is and/or might be following by polymerization to get the desired length of polymer chain.
In the preferred embodiment of this invention, the said new subtances I can form a complex substance with metal.
In the further aspect of this invention describing the method of membrane porosity enlargement of simple cells or another organic membranes with some effective amount of this invention.
In the preferred embodiment of this invention, the new subtances of this invention to be discovered have the capability to modify membrane of simple cells or organic membranes by mean enlarging significantly the membrane porosity. By not theoretically fully supported, it is postulated due to sulphate group electronegativity of this sulphate chelating agents will tense the hydroxy-hydroxy bound of peptidoglycan and/or hydroxy-ammine bound of protein that compose cell membranes or organic membranes. The capability of these said subtances are proven by mean of opening/enlarging membrane porosity of some microorganisms simple cell, such as: bacteria cell, algae, fungi, and similar with some concentration level worked on the membrane modification mechanism from semi permeable into permeable membrane therefore it is become possible for another ligand insertion into target microorganism and/or simple cell; also possible that the microrganism cell wall membranes become totally permeable result on the microorganism death due to cell internal lysis or dorment/latent condition of the target microorganism. It is postulated that this said mechanism works for almost all of microorganism having cell membranes.
a and 2b: are the titration curves show the comparation of chelating capability between THES and EDTA, where it needs less THES concentration in comparation to EDTA.
In such embodiment of subtances have the formula
wherein:
X1, X2, X3, are selected from: Hydrogen, hydroxy, halide, sulphite, sulphate, sulphonate, phosphorus derivatives, nitrogen derivatives, where X1, X2, X3, could be similar or different atoms, molecules or groups.
Rx, Rz are selected from: Hydrogen, hydroxy, halide, alkyl C1-20, alkylene C1-20, alkyl alcohol C1-20, aliphatic or branched substituted or unsubstituted, aryl, cycloalkyl substituted or unsubstituted, sulphite, sulphate, sulphonate, phosphorus derivatives, nitrogen derivatives.
M: are Hydrogen, or group I, group II, transition group cation that are pharmaceutically acceptable.
n: intreger from 0-3
a: intreger
and/or its isomer, enantiomer, stereotiomer, salt, solvate, hydrate that are pharmaceutically acceptable.
In the other preferred embodiment of subtances derived from Formula I where M is hydrogen.
In the most preferred embodiment of substance is
and/or its isomer, enantiomer, stereotiomer, salt, solvate, hydrate that are pharmaceutically accepted.
In the other preferred embodiment of such subtances from Formula I, where M is Natrium.
In the preferred embodiment of the above subtances are:
and/or its isomer, enantiomer, stereotiomer, salt, solvate, hydrate that are pharmaceutically accepted.
Furthermore, the formed alkyl sulphate subtances derived from formula I are still enabled to be polymerized to form longer chain.
In the preferred embodiment of this invention covers breaking or loosening the hydroxyl-hydroxyl bound of peptidoglycan or hydroxyl-ammine bound of protein that compose cell wall membranes or organic membranes by mean utilizing some sulphate chelating agent that result on the enlargement of the membrane porosity, therefore the membrane become permeable on both of its sides. The capability of the said subtances was proven by opening/enlargement the microorganism simple cell's membrane, such as: bacteria, algae, fungi, virus, etc with some concentration level that works on the cell wall membrane modification from semi permeable membrane into more and more permeable, therefore it becomes possible to insert another ligands inside the microorganism or the cell wall membrane become totally permeable that result on the microorganism death and/or microorganism dorment condition. It is postulated the said mechanism works for all types of microorganism that has cell membrane.
The following terms are used throughout the specification and have the following meanings unless otherwise specified:
“Alkyl” means carbon atom chains having the designated number of carbon atoms which can be either straight chain or branched. Examples of alkyl include but are not limited to, methyl, ethyl, propyl, butyl, isobutyl, and the like.
“Alkenyl” means carbon atom chains having the designated number of carbon atoms which can be either straight chain or branched and which contain at least one double bond. The alkenyl compounds may have more than one such double bond and the orientation about each double bond is independently either cis or trans. Examples of alkenyl include, but are not limited to ethenyl, propenyl, butenyl, isobutenyl, pentenyl, hexenyl and the like.
“Alkynyl” means carbon atom chains having the designated number of carbon atoms which can be either straight chain or branched and which contain at least one triple bond.
As used herein the term “aryl” means single, polynucleic conjugated and fused residues of aromatic hydrocarbon or aromatic heterocyclic ring systems, example as of aryl include, but are not limited to phenyl, naphthyl, fluorenyl, pyrinyl, pyridyl, pyrollyl, and the like.
Cycloalkyl termination refers to non-aromatic aliphatic ring moiety of 3-20 mono-cyclic, bicyclic, or polycyclic carbon atoms. Cycloalkyl can be bicycloalkyl, polycycloalkyl, branched, or spiroalkyl. One or more of the rings might have one or more double bond but no such ring has fully conjugated pi-electron system. Example, without limitation, of cycloalkyl group are: cyclopropane, cyclobutane, cyclopentane, cyclopenthenyl, cyclohexane, cyclohexadiene, adamanthane, cycloheptane, cycloheptatriene, and the like.
Sulphite structure could be described with three equal resonance structures. In every resonance structure, the sulphur atom has a double bond with one oxygen atom with zero/neutral charge, and the single sulphur atom is bound into two other oxygen atoms that each has formal charge of −1. It is also found free electrons pair on Sulphur atom, therefore the predicted structure by VSEPR theory is a pyramid trigonal similar to ammonia (NH3).
Sulphate termination refers to sulphur as the centered atom surrounded by four equal oxygen atoms in tetrahedral structure. Sulphur atom in its oxidation state +6 while the four oxygen atoms, each in its oxidation state −2. Sulphate ion carry negative charge and two of them are the alkaline conjugate of bisulphate (or hydrogen sulphate), HSO4−, as the alkaline conjugate of H2SO4, sulphuric acid.
Sulphonate termination refers to an ester of sulphonic acid. An ester with the common formula ROSO2R′ is a sulphonic ester. The common structures of sulphonic ester shown by the structures below:
Nitrogen derivatives termination refers to carbon subtances that contain nitrogen atom, cyclical or aliphatic, straight or branched chain, if it has cyclical structure, then it could be in fusion or non fusion form, could be aromatic or non aromatic.
Phosphorous derivatives termination refers to carbon subtances that contain phosphorous atom, cyclical or aliphatic, straight or branched chain, if it has cyclical structure, then it could be in fusion or non fusion form, could be aromatic or non aromatic.
Pharmaceutically accepted salts refer to alkaline addition types of salts synthesized by adding some alkaline subtances to the said subtances in this invention. The common pharmaceutically accepted salts are: natrium, kalium, calcium or zinc.
In the specification, the termination of substituted means that a group may be further substituted with one or more groups selected from alkyl, alkenyl, alkunyl, aryl, fluoro, chloro, bromo, hydroxyl, alkoxy, alkenyloxy, aryloxy, acyloxy, amino, alkylamino, dialkylamino, arylamino, thio, alkylthio, arylthio, cyano, nitro, acyl, amido, alkylamido, dialkylamido, carboxyl or two or more subtituens may, together with the carbon atoms to which they are attached from a 5 or 6 membered aromatic or non aromatic ring containing 0, 1 or 2 heteroatoms selected from nitrogen, oxygen and sulphur.
Main selection of the preferred subtances of this invention are:
wherein:
X1, X2, X3, are selected from: Hydrogen, hydroxy, halide, sulphite, sulphate, sulphonate, phosphorus derivatives, nitrogen derivatives, where X1, X2, X3, could be similar or different atoms, molecules or groups.
Rx, Rz are selected from: Hydrogen, hydroxy, halide, alkyl C1-20, alkylen C1-20, alkyl alcohol C1-20, aliphatic or branched substituted or unsubstituted, aryl, cycloalkyl substituted or unsubstituted, sulphite, sulphate, sulphonate, phosphorus derivatives, nitrogen derivatives;
a: intreger;
with sulphuric acid (H2SO4) and/or sulphonic acid (H2SO3) and/or sulphur trioxide (SO2) and/or sulphur dioxide (SO2),
Examples of the Synthesis of Some Subtances within this Invention:
225 g of oxalic acid crystal was diluted in 150 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 250 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation until the next 180 minutes with the installation of vertical pipe condensor for reflux purposes to maintain the volume of the liquid until a very clear solution is achieved.
Then the liquid temperature was raised to 90-100° C. Evaporation of the solvent followed by crystallization result in formation of the desired substance as an off white powder with the following properties: melting point >200° C., boiling point >400° C., specific gravity=1.86, water solubility 48%.
90 g of oxalic acid crystal was diluted in 150 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 100 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry heated with agitation until the next 180 minutes with the installation of vertical pipe condensor for reflux was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation until the next 180 minutes with the installation of vertical pipe condensor for reflux purposes to maintain the volume of the liquid until a very clear solution is achieved.
To this clear solution was added dropwise a solution of 73 g NaOH flakes in 75 ml of water with the rate of addition being controlled so that the temperature of the solution remained at 50±20° C. Upon completion of addition, the temperature was adjusted slowly to ambient and the solution was held agitated in the ambient temperature for the next 60 minutes.
Evaporation of the solvent followed by crystallization result in formation of the desired substance as an off white powder with the following properties: melting point >200° C., boiling point >400° C., specific gravity=2.2, water solubility 31%.
184 g formic acid crystal was diluted in 300 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 230 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation for the next 60 minutes, then 8.6 gram of natrium peracetate was added as the catalyst for polymerization process with the installation of vertical pipe condenser during the next 120 minutes reflux until a very clear solution is achieved. Then the temperature was adjusted slowly to ambient and the solution was held agitated in the ambient temperature for the next 60 minutes.
Upon 60 minutes agitation completion, the condenser was released, then the solution is boiled for around 2 hours to evaporate the water until 35-40% of its original volume evaporated. The resulting solution then undergoes slow cooling into ambient temperature and cooling further into ±10° C. by ice water bath. The resulting suspension then is filtrated to separate the Tetra Hydroxy Butyl 1,2,3,4 Tetra Sulphate acid crystals, the separated crystals then oven dried at ±70-80° C. until the constant weight reached.
184 g formic acid crystal was diluted in 300 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 230 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation for the next 60 minutes, then 8.6 gram of natrium peracetate was added as the catalyst for polymerization process with the installation of vertical pipe condenser during the next 120 minutes reflux until a very clear solution is achieved. To this clear solution was added dropwise a solution of 170 g NaOH flakes in 200 ml of water with the rate of addition being controlled so that the temperature of the solution remained at 50±20° C. Upon completion of addition, the temperature was adjusted slowly to ambient and the solution was held agitated in the ambient temperature for the next 60 minutes.
Upon 60 minutes agitation completion, the condenser was released, then the solution is boiled for around 2 hours to evaporate the water until 35-40% of its original volume evaporated. The resulting solution then undergoes slow cooling into ambient temperature and cooling further into ±10° C. by ice water bath. The resulting suspension then is filtrated to separate the Tetra Hydroxy Butyl 1,2,3,4 Tetra Sulphate Tetra Natrium crystals, the separated crystals then oven dried at ±70-80° C. until the constant weight reached.
138 g formic acid crystal was diluted in 300 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 173 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation for the next 60 minutes, then 8.6 gram of natrium peracetate was added as the catalyst for polymerization process with the installation of vertical pipe condenser during the next 120 minutes reflux until a very clear solution is achieved. Next, prepare methanol solution of 34 g methanol 96% technical grade in 60 mL aquadess; into this solution, 43 g of Caustic soda flakes was added following by 30 minutes agitation, until the salt precipitated perfectly.
Then the said hot liquid was cooling slowly into ambient temperature while still agitated for the next 60 minutes. Separate the precipitated salt through filter to get the clear filtrate.
Upon the filtration completion, the condenser was released, then the solution is boiled for around 2 hours to W evaporate the water until 35-40% of its original volume evaporated. The resulting solution then undergoes slow cooling into ambient temperature and cooling further into ±10° C. by ice water bath. The resulting suspension then is filtrated to separate the Tri Hydroxy Butyl 1,2,3 Tri Sulphate acid; the separated crystals then oven dried at ±70-80° C. until the constant weight reached.
138 g formic acid crystal was diluted in 300 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 173 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation for the next 60 minutes, then 8.6 gram of natrium peracetate was added as the catalyst for polymerization process with the installation of vertical pipe condenser during the next 120 minutes reflux until a very clear solution is achieved. Next, prepare methanol solution of 34 g methanol 96% technical grade in 60 mL aquadess; into this solution, 43 g of Caustic soda flakes was added following by 30 minutes agitation, until the salt precipitated perfectly.
Then the said hot liquid was cooling slowly into ambient temperature while still agitated for the next 60 minutes. Separate the precipitated salt through filter to get the clear filtrate.
To this clear solution was added dropwise a solution of 130 g NaOH flakes in 200 ml of water with the rate of addition being controlled so that the temperature of the solution remained at 50±20° C.
Upon completion, the condenser was released, then the solution is boiled for around 2 hours to evaporate the water until 35-40% of its original volume evaporated. The resulting solution then undergoes slow cooling into ambient temperature and cooling further into ±10° C. by ice water bath. The resulting suspension then is filtrated to separate the Tri Hydroxy Butyl 1,2,3 Tri Sulphate-3Natrium crystals; the separated crystals then oven dried at ±70-80° C. until the constant weight reached.
184 g formic acid crystal was diluted in 300 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 230 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation for the next 60 minutes, then 8.6 gram of natrium peracetate was added as the catalyst for polymerization process with the installation of vertical pipe condenser during the next 120 minutes reflux until a very clear solution is achieved. Next, prepare methanol solution of 34 g methanol 96% technical grade in 60 mL aquadess; into this solution, 43 g of Caustic soda flakes was added following by 30 minutes agitation, until the salt precipitated perfectly.
Then the said hot liquid was cooling slowly into ambient temperature while still agitated for the next 60 minutes. Separate the precipitated salt through filter to get the clear filtrate.
Upon the filtration completion, the condenser was released, then the solution is boiled for around 2 hours to evaporate the water until 35-40% of its original volume evaporated. The resulting solution then undergoes slow cooling into ambient temperature and cooling further into ±10° C. by ice water bath. The resulting suspension then is filtrated to separate the Tetra Hydroxy Isoamyl 1,2,3,4 Tetra Sulphate acid; the separated crystals then oven dried at ±70-80° C. until the constant weight reached.
184 g formic acid crystal was diluted in 300 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 230 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation for the next 60 minutes, then 8.6 gram of natrium peracetate was added as the catalyst for polymerization process with the installation of vertical pipe condenser during the next 120 minutes reflux until a very clear solution is achieved.
Next, prepare methanol solution of 34 g methanol 96% technical grade in 60 mL aquadess; into this solution, 43 g of Caustic soda flakes was added following by 30 minutes agitation, until the salt precipitated perfectly.
Then the said hot liquid was cooling slowly into ambient temperature while still agitated for the next 60 minutes. Separate the precipitated salt through filter to get the clear filtrate.
To this clear solution was added dropwise a solution of 170 g NaOH flakes in 200 ml of water with the rate of addition being controlled so that the temperature of the solution remained at 50±20° C.
Upon completion, the condenser was released, then the solution is boiled for around 2 hours to evaporate the water until 35-40% of its original volume evaporated. The resulting solution then undergoes slow cooling into ambient temperature and cooling further into ±10° C. by ice water bath. The resulting suspension then is filtrated to separate the Tetra Hydroxy Isoamyl 1,2,3,4 Tetra Sulphate-4Natrium; the separated crystals then oven dried at ±70-80° C. until the constant weight reached.
75 g of oxalic acid crystal was diluted in 75 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 250 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry heated with agitation until the next 180 minutes with the installation of vertical pipe condensor for reflux was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation until the next 120 minutes with the installation of vertical pipe condensor for reflux purposes to maintain the volume of the liquid until a very clear solution is achieved; then the vertical pipe condensor was released, the resulting solution is still heated with another 30 minutes agitation to ensure complete and homogenous sulphatation reaction.
Then the liquid temperature was raised to 100-105° C. Evaporate half of the solvent volume followed by crystallization result in formation of the desired substance as an off white powder with the following properties: melting point >210° C., boiling point >400° C., specific gravity=1.87, water solubility 48% and very higroscopic.
75 g of oxalic acid crystal was diluted in 75 ml of water and the resulted slurry heated to 40-50° C. To this slurry was added dropwise 250 ml of 98% H2SO4. The rate of acid addition was controlled to ensure that the temperature of the slurry heated with agitation until the next 180 minutes with the installation of vertical pipe condensor for reflux was in the range of 90-100° C. at atmospheric pressure. Upon completion of H2SO4 acid addition, the solution is still heated with agitation until the next 120 minutes with the installation of vertical pipe condensor for reflux purposes to maintain the volume of the liquid until a very clear solution is achieved; then to this clear solution was added dropwise a solution of 185 g NaOH flakes in 190 ml of water with the rate of addition being controlled so that the temperature of the solution remained at 50±20° C. within one hours time. Then the vertical pipe condensor was released, the resulting solution is still heated with another 30 minutes agitation to ensure complete and homogenous neutralization reaction.
Then the liquid temperature was raised to 100-105° C. Evaporate half of the solvent volume followed by crystallization result in formation of the desired substance as an off white powder with the following properties: melting point >360° C., boiling point >400° C., specific gravity=2.21, water solubility 29% and higroscopic.
Active matter of Tetra Hydroxy Ethyl Di Sulphate-Di Natrium (THES-2Na) was determined by chelating reaction of residual metallic ion with several anionic surfactans that prevent this anionic surfactans dissolved in the solution result in the turbid solution measured by its turbidity and/or absorbance spectrophotometer.
Reagent grade NH3FeSO4.2H2O, reagent grade Natrium Laurier Ethoxylate sulphate (Na-SLES), Caustic Soda flakes, aquadess.
Visible Spectrofotometer, cuvet, erlen meyer, beaker glass, pipete, scale weight, magnetic stirrer.
Take 1.0 cc of 35% Tetra Hidroksi Etil Di Sulfat Di Natrium (THES-2Na) solution as sample, density of 1.1 g/cc. Then add on Na-SLES, titrate with 0.1 M NH3FeSO4; the absorbance record shown in the Table 1 below:
From Table 1 above, the absorbance curve versus volume NH3FeSO4 trend is shown in
From
THES-2Na concentration=3.2 cc×0.1 M×330×3.9/(1.0×1.15×1,000)=0.358=35.8% of chelating agent.
Characteristics of this New Invention Subtances
These subtances of this new invention are preferred to have the characteristics as below:
The subtances of this invention were found to have the ability to modify the cell membrane or organic membrane by means enlarging/loosening membrane porosity significantly. By not totally theoretically supported, it was postulated due to W the sulphate group electronegativity in the said subtances as the chelating agent will loosen the hydroxy-hydroxy bound of a peptidoglycan or hydroxy-ammine bound of a protein that compose the membrane cell nor organic membrane. The ability of these subtances to enlarge/loosen the membrane porosity were proven by opening/enlarging the microorganism cell membrane, ie: bacteri, algae, fungi, virus, etc with such concentration level that works on the cell wall membrane modification from the semi permeable into more permeable until it is possible to insert another ligands inside the microorganism or the cell wall membrane becomes totally permeable that result on the microorganism death or its latent position. It has been thought that the said mechanism works for all microorganism that has the cell membrane. The said ability to modify the simple cell membrane permeability will be shown by the mechanism and testing data below:
The ability of Tetra Hydroxy Ethyl Di Sulphate-Di Natrium W (THES) as a Sulphate chelating agent to open/loosen the cell membrane was tested against two types of bacterias, ie: Staphylococcus aerus (gram positive) that has two cell membrane (the outer membrane as the outer cell wall and the inner membrane) and E. Coli (gram negative) that has three cell membranes. First, the Minimum Inhibitory Concentration (MIC) of each bacterias was determined, then the bacteria membrane alteration after contacting with THES were observed by Electron Microscope (TEM and SEM) with the THES concentration range below and above MIC. Antibacterial activity was tested using disc-diffusion method by checking the clear zone from the paper disc inhibition that contains THES. The growth curve were made by means examining the bacterial culture on the broth media.
Difco brand nutrient broth, aquadistilata, THES (tetra hydroxy ethyl di sulphate di natrium) as the tested substance, glutaraldehyde, paraformaldehyde, albumin serum bovin (BSA), buffer phosphate pH 7, NaCl 0.9%, white LR resin, blue toluidine, Natrium citrate trihydrate, NaOH 0.1N, uranil acetate, lead nitrate (Pb(NO3)2), paraffin, transparan capsule, glass cutter, grid, collodion, gold powder.
Incubator, autoclave, petri dish, ose, spectrophotometer (Bausch&Lomb), vibrating incubator, glassware, ultra mikrotom, knife maker, visible microscope, grid pad, transmission electron microscope (Philips), scanning electron microscope (Hitachi).
E. Coli
Staphylococcus Aerus
Staphylococcus aerus ATCC 6538, Escherichia coli ATCC 9637 were supplied from Chemotherapy Laboratory of Pharmaceutical Department, Bandung Institute of Technology (ITB).
MIC of tetra hydroxy ethyl di sulphate di natrium against E. coli and S. aureus are shown in Table 3.
Bacillus Subtilis ATCC
Staphylococcus Aerus ATCC
Staphylococcus Epidermidis
Acinetobacter Anitratus
Escherichia Coli ATCC
Pseudomonas Aeruginosa
Klebsiella Pneumoniae
Salmonella Typhii
Aspergillus Niger
Candida Albicans
The test done on the pH range of 7-7.5 with the sample of Tetra Hydroxy Ethyl Di Sulphate-Di Natrium (THES-Di Natrium) 30% in aquaeus solution.
From the transversal section (TEM) of Staphylococcus aerus cell, can be observed between normal cell that still contains cytoplasm and other organic subtances (dark sight) and the partly empty cells that has already lost its cytoplasm liquid due to lysis out of cell wall membrane (transparent W sight), in fact the E. Coli cell that has more layers cell wall membranes also experienced the said internal cell liquid lysis out of its cell wall membrane layers, although the MIC of E. Coli cell was slightly higher than Staphylococcus aerus cell.
From the outer surface of bacteria's cell wall observation through Scanning Electron Microscope (SEM), it was shown that the outer cell wall membrane of bacteria looked wrinkled/corrugated due to the wider cell wall surface compared to normal cell wall for both Staphylococcus aerus nor E. Coli.
| Number | Date | Country | Kind |
|---|---|---|---|
| P 00 2012 01174 | Dec 2012 | ID | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/ID2013/000009 | 11/22/2013 | WO | 00 |
