SUPEROXIDE DISMUTASE COMPOSITIONS AND METHODS

Abstract

The present disclosure is drawn to superoxide dismutase (SOD) compositions and methods thereof. A composition can comprise a combination of a therapeutically effective amount of superoxide dismutase (SOD) with a stabilizing carrier. The SOD can be provided by a natural source that can be a botanical source. A method of treating a condition in a subject that is responsive to treatment with superoxide dismutase (SOD) can comprise administering a therapeutically effective amount of the composition. A method of stabilizing a superoxide dismutase (SOD) composition can comprise combining an amount of SOD with deoxygenated water to form an SOD solution and minimizing exposure of the SOD solution to reactive oxygen species (ROS).

Description

Claims

1. A composition, comprising: a combination of a therapeutically effective amount of superoxide dismutase (SOD) provided by a natural source with a stabilizing carrier.

2. The composition of claim 1, wherein the natural SOD source is a botanical source.

3. The composition of claim 2, wherein the botanical SOD source is an extract of one or more species from the genus Rosa, an extract of one or more species from the genus Silphium L ., an extract of one or more species from the genus Glycine, or a combination thereof.

4. The composition of claim 3, wherein the Rosa species is a member selected from the group consisting of: R. abietina,R. abutalybovii,R. abyssinica,R. achburensis,R. acicularis,R. adenophylla,R. agrestis,R. alabukensis,R. alberti,R. alexeenkoi,R. altidaghestanica,R. amblyophylla,R. antonowii,R. arabica,R. arensii,R. arkansana,R. arvensis,R. awarica,R. baiyushanensis,R. balcarica,R. balsamica,R. balsamica,R. banksiae,R. banksiopsis,R. beauvaisii,R. beggeriana,R. bella,R. bellicose,R. biebersteiniana,R. blanda,R. boissieri,R. borissovae,R. bracteate,R. bridgesii,R. brotherorum,R. brunonii,R. bugensis,R. buschiana,R. caesia,R. calanthe,R. calcarean,R. californica,R. calyptopoda,R. canina,R. Carolina,R. caryophyllacea,R. cathayensis,R. caudata,R. chengkouensis,R. chinensis,R. chionistrae,R. clinophylla,R. corymbifera,R. corymbulosa,R. coziae,R. crenatula,R. crocacantha,R. cziragensis,R. daishanensis,R. darginica,R. davidii,R. davurica,R. deqenensis,R. derongensis,R. deseglisei,R. didoensis,R. diplodonta,R. dolichocarpa,R. doluchanovii,R. donetzica,R. dsharkenti,R. duplicate,R. ecae,R. elasmacantha,R. elymaitica,R. ermanica,R. facsarii,R. fargesiana,R. farreri,R. filipes,R. foetida,R. foliolosa,R. forrestiana,R. freitagii,R. fujisanensis, R. gadzhievii,R. gallica,R. galushkoi,R. giraldii,R. glabrifolia,R. glandulososetosa,R. glauca,R. glomerata,R. gorenkensis,R. graciliflora,R. gracilipes,R. gymnocarpa,R. hawrana,R. heckeliana,R. helenae,R. hemisphaerica,R. henryi,R. hezhangensis,R. hirsute,R. hirtissima,R. hirtula,R. horrida,R. hracziana,R. iberica,R. iliensis,R. iljinii,R. indica,R. inodora,R. irinae,R. irysthonica,R. isaevii,R. jaroshenkoi,R. juzepczukiana,R. kamelinii,R. karaalmensis,R. kazarjanii,R. khasautensis,R. kokanica,R. kokijrimensis,R. koreana,R. koslowskii,R. kossii,R. kuhitangi,R. kujmanica,R. kunmingensis,R. kwangtungensis,R. kweichowensis,R. laevigata,R. langyashanica,R. lapidosa,R. lasiosepala,R. laxa,R. lehmanniana,R. leschenaultiana,R. lichiangensis,R. lonaczevskii,R. longicuspis,R. longisepala,R. lucidissima,R. lucieae,R. ludingensis,R. lupulina,R. macrophylla,R. maeotica,R. mairei,R. majalis,R. mandenovae,R. mandonii,R. marginate,R. maximowicziana,R. memoryae,R. mesatlantica,R. micrantha,R. minutifolia,R. miyiensis,R. mollis,R. montana,R. morrisonensis,R. moschata,R. moyesii,R. multibracteata,R. multiflora,R. murielae,R. nipponensis,R. nitida,R. nutkana,R. obtegens,R. obtusiuscula,R. odorata,R. omeiensis,R. onoei,R. opaca,R. oplisthes,R. orientalis,R. osmastonii,R. ossethica,R. oxyacantha,R. oxyodon,R. palustris,R. paniculigera,R. pedunculata,R. penduline,R. persetosa,R. persica,R. Phoenicia,R. pinetorum,R. pinnatisepala,R. pisocarpa,R. platyacantha,R. popovii,R. pouzinii,R. praelucens,R. praetermissa,R. prattii,R. pricei,R. primula,R. prokhanovii,R. pseudobanksiae,R. pseudoscabriuscula,R. pubicaulis,R. pulverulenta,R. pygmaea,R. rapinii,R. rhaetica,R. roopiae,R. roxburghii,R. rubiginosa,R. rubus,R. rugosa,R. russanovii,R. sambucina,R. saturate,R. saundersiae,R. schergiana,R. schrenkiana,R. sempervirens,R. serafinii,R. sericea,R. sertata,R. setigera,R. setipoda,R. shangchengensis,R. sherardii,R. sikangensis,R. simplicidens,R. sinobiflora,R. sjunikii,R. Sogdiana,R. soulieana,R. spinosissima,R. spithamea,R. squarrosa,R. stellate,R. stylosa,R. subbuschiana,R. taiwanensis,R. taronensis,R. tchegemensis,R. teberdensis,R. tesquicola,R. tibetica,R. tlaratensis,R. tomentosa,R. transcaucasica,R. transmorrisonensis,R. transsilvanica,R. tschimganica,R. tsinlingensis,R. tunquinensis,R. turcica,R. turkestanica,R. tuschetica,R. uniflora,R. uniflorella,R. usischensis,R. vagiana,R. vassilczenkoi,R. villosa,R. virginiana, R. webbiana,R. weisiensis,R. willmottiae,R. woodsii,R. xanthina,R. zakatalensis,R. zalana,R. zaramagensis,R. zhongdianensis,R. zuvandica, or a combination thereof.

5. The composition of claim 4, wherein the Rosa species is Rosa roxburghii.

6. The composition of claim 4, wherein the Rosa extract has a standardized SOD content ranging from about 1,000 U/g to about 50,000 U/g.

7. The composition of claim 3, wherein the Silphium L . species is a member selected from the group consisting of: S. albiflorum,S. asteriscus,S. brachiatum,S. compositum,S. glutinosum,S. integrifolium,S. laciniatum,S. laeve,S. mohrii,S. perfoliatum,S. perplexum,S. pinnatifidum,S. radula,S. terebinthinaceum,S. ternatum,S. trifoliatum,S. wasiotense, or a combination thereof.

8. The composition of claim 7, wherein the Silphium L . species is Silphium perfoliatum L .

9. The composition of claim 7, wherein the Silphium extract has a standardized SOD content ranging from about 500 U/g to about 50,000 U/g.

10. The composition of claim 3, wherein the Glycine species is a member selected from the group consisting of: Glycine albicans,Glycine aphyonota,Glycine arenaria,Glycine argyrea,Glycine canescens,Glycine clandestina,Glycine curvata,Glycine cyrtoloba,Glycine falcata,Glycine gracei,Glycine hirticaulis, G. hirticaulis subsp. leptosa,Glycine lactovirens,Glycine latifolia,Glycine latrobeana,Glycine microphylla,Glycine montis-douglas,Glycine peratosa,Glycine pescadrensis,Glycine pindanica,Glycine pullenii,Glycine remota,Glycine rubiginosa,Glycine stenophita,Glycine syndetika,Glycine tabacina,Glycine tomentella,Subgenus Soja,Glycine soja,Glycine max, or a combination thereof.

11. The composition of claim 10, wherein the Glycine species is Glycine max or Glycine soja.

12. The composition of claim 10, wherein the Glycine extract has a standardized SOD content ranging from about 500 U/mg to about 50,000 U/mg.

13. The composition of claim 1, further comprising a vitamin selected from the group consisting of: vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin H, vitamin K, folic acid, and a combination thereof in an amount of from about 1 wt% to about 10 wt% of the composition.

14. The composition of claim 1, further comprising a polyphenol selected from the group consisting of: a flavonoid, a tannin, and a combination thereof in an amount of from about 0.0001 wt% to about 25 wt% of the composition.

15. The composition of claim 1, further comprising an antioxidant.

16. The composition of claim 1, wherein the composition is water soluble.

17. The composition of claim 1, further comprising a pharmaceutically acceptable carrier.

18. The composition of claim 1, wherein when the SOD is present in the composition at a concentration of 20,000 U/g: more than about 80% of the SOD remains in a reactive form after a period of about 2 weeks when stored at 25° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 2 weeks when stored at 40° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 4 weeks when stored at 25° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 4 weeks when stored at 40° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 6 months when stored at 25° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 6 months when stored at 40° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 2 years when stored at 25° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 4 years when stored at 25° C. and 75% relative humidity.

19. The composition of claim 1, wherein when the SOD is present in the composition at a concentration of 5,000 U/g: more than about 80% of the SOD remains in a reactive form after a period of about 2 weeks when stored at 25° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 2 weeks when stored at 40° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 4 weeks when stored at 25° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 4 weeks when stored at 40° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 6 months when stored at 25° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 6 months when stored at 40° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 2 years when stored at 25° C. and 75% relative humidity; ormore than about 80% of the SOD remains in a reactive form after a period of about 4 years when stored at 25° C. and 75% relative humidity.

20. The composition of claim 1, wherein the SOD comprises SOD-1, SOD-2, SOD-3, or a combination thereof.