Claims
- 1. A composition comprising a surface, said surface comprising a coating, said coating comprising a linker, wherein said linker has a first end covalently coupled to said surface and a second end comprising a reactive group, wherein said linker further comprises a hydrophobic portion and a hydrophilic portion, wherein said hydrophobic portion is configured to collapse in an aqueous environment so as to increase stability of attachment of said linker to said surface.
- 2. The composition of claim 1, wherein said surface comprises a glass surface.
- 3. The composition of claim 2, wherein said coating comprises sol-gel glass.
- 4. The composition of claim 1, wherein said linker is synthesized using Atom Transfer Radical Polymerization.
- 5. The composition of claim 1, wherein said reactive group permits attachment of a nucleic acid molecule to said second end of said linker.
- 6. The composition of claim 1, further comprising a nucleic acid molecule attached to said second end of said linker.
- 7. The composition of claim 1, further comprising 100 or more nucleic acid molecules attached to said surface.
- 8. A composition comprising a surface, said surface comprising a hydrophobic coating, said hydrophobic coating comprising a plurality of oxidize spots, said oxidized spots produced by a method comprising:
a) coating said surface with compounds containing disulfide bonds to generate said hydrophobic coating; and b) exposing said hydrophobic coating in a plurality of spots with an oxidizing agent to generate said plurality of oxidized spots.
- 9. The composition of claim 8, wherein said surface comprises a glass surface.
- 10. The composition of claim 8, wherein said coating comprises sol-gel glass.
- 11. The composition of claim 8, wherein said oxidizing agent comprises hydrogen peroxide.
- 12. The composition of claim 8, wherein said surface comprises a nucleic acid molecule attached to said surface in one or more of said plurality of oxidized spots.
- 13. A method comprising;
a) providing;
i) a solid support comprising a well, ii) a non-aqueous liquid, and iii) a detection reagent solution; and b) adding said non-aqueous liquid to said well, and c) adding said detection reagent solution to said well through said non-aqueous liquid under conditions such that at least one microarray-spot is formed in said well.
- 14. The method of claim 13, further comprising step d) contacting said at least one microarray-spot with a test sample solution.
- 15. The method of claim 14, wherein said contacting comprises propelling said test sample solution through said non-aqueous liquid in said well.
- 16. The method of claim 13, wherein said non-aqueous liquid is oil.
- 17. The method of claim 13, wherein said solid support comprises a plurality of wells, and the method is performed with said plurality of wells.
- 18. The method of claim 17, wherein at least two microarray-spots are formed simultaneously.
- 19. The method of claim 14, wherein said test sample solution comprises a target nucleic acid molecule.
- 20. The method of claim 19, wherein said target solution comprises less than 800 copies of a target nucleic acid molecule.
- 21. The method of claim 19, wherein said contacting said microarray-spot with said test sample solution identifies the presence or absence of a polymorphism in said target nucleic acid molecule.
- 22. The method of claim 13, wherein said well is coated with a sol-gel coating.
- 23. The method of claim 14, wherein said contacting is performed with a CARTESIAN SYNQUAD nanovolume pipetting system.
Parent Case Info
[0001] The present application claims priority to U.S. Provisional Application Serial No. 60/361,108 filed Feb. 27, 2002 and U.S. Provisional Application Serial No. 60/436,199 filed Dec. 23, 2002, both of which are herein incorporated by reference.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60361108 |
Feb 2002 |
US |
|
60436199 |
Dec 2002 |
US |