Patent Application
20050223806
It is well established that surfaces can support mechanical waves. Depending on the nature of the substrate, these surface waves are called Rayleigh waves, Lamb waves, and Love waves. Rayleigh waves are for thicker substrates while Lamb and Love waves are for thin membranes. The surface waves can be generated on a piezoelectric substrate using electrode arrays deposited on the surface. Once created, the wave travels on the surface and can be detected using a second electrode array. If the surface waves encounter adsorbed species, the frequency of the wave varies very sensitively due to mass or stress loading. The frequency of the wave can be very high—hundreds of MHz—yet the frequency and phase can be measured very precisely to obtain extremely high sensitivity.
One important disadvantage of the above surface-wave sensor is its inability to distinguish between different chemical species. This disadvantage also exists on other mass sensors such as the quartz crystal microbalance (QCM), plate wave resonator, and Lamb and Love wave sensors. To overcome this disadvantage of lack of chemical selectivity, SAW sensors are generally coated with a chemically selective layer. However, this chemically selective layer frequently does not provide good chemical selectivity except in rare cases, for example, biosensors based on antibody-antigen interaction or DNA hybridization. Polymeric coatings are used in certain vapor sensors and are sensitive to a variety of analytes depending upon the solubility of the analyte in each polymer. To provide unambiguous detection of even a single analyte in a mixture that may contain other analyte vapors, for example, arrays of sensors with various polymers are required to provide even qualitative information about the nature of the adsorbates and thus the vapors.
Surface waves can also be extremely sensitive to temperature, depending upon the nature of the piezoelectric substrate. With appropriate material, small changes in temperature can cause rather large changes in frequency, wave velocity, and phase. In this invention, we exploit this extremely high sensitivity of surface waves to temperature and other physical properties to achieve chemical selectivity using photo-absorption. In addition to temperature, the surface wave velocity is affected by pressure, surface stress, magnetic and electric fields. This invention exploits changes in surface wave velocity due to variation in surface temperature, pressure, surface stress, electric and magnetic field induced by interaction of adsorbate with impinging electromagnetic radiation on the sensor surface. A variation in surface acoustic wave properties such as frequency, velocity, phase, amplitude, and Q-factor as a function of the wavelength of the impinging electromagnetic wave is used as a signatures for speciation of adsorbed species.
U.S. Pat. No. 6,180,415 to Schultz et al.; U.S. Pat. No. 6,331,276 to Takei et al.; and U.S. Pat. No. 6,515,749 to Pipino teach sensor devices and methods using only optical parameter changes for detection. U.S. Pat. No. 5,923,421 to Rajic et al., herein incorporate by reference teaches similar absorption spectrometry devices and methods using bolometers, thermopiles, pyroelectrics or microcantilever sensing elements.
A surface wave chemical detector comprising at least one surface wave substrate, each of said substrates having a surface wave and at least one measurable surface wave parameter; means for exposing said surface wave substrate to an unknown sample of at least one chemical to be analyzed, said substrate adsorbing said at least one chemical to be sensed if present in said sample; a source of a radiation for respectively radiating and thereby heating said surface wave substrate with different wavelengths of said monochromatic spectrum, said surface wave parameter being changed by said adsorbing; and means for recording signals representative of said surface wave parameter of each of said surface wave substrates responsive to said radiation of said different wavelengths, measurable changes of said parameter due to adsorbing said chemical defining a unique signature of a detected chemical.
This invention gains chemical selectivity by exposing the surface with adsorbed chemical species (adsorbate) to certain wavelengths that are indicative of the species present. High sensitivity of surface waves is combined with chemical selectivity of absorption spectroscopy. This approach does not necessarily depend upon coatings, thus greatly improving selectivity and avoiding the long-standing issues related to degradation of external coatings.
An apparatus and method is taught that combines the extreme high sensitivity of surface wave sensors with high selectivity of absorption spectroscopy producing chemically selective surface wave sensors.
Chemical analytes adsorb at certain frequencies of electromagnetic radiation. Absorption spectroscopy is a standard technique used to identify and quantify chemical species, for example, Fourier-transform infrared spectroscopy. This invention combines the high sensitivity of surface waves to small changes in temperature and other physical properties with optical spectroscopy for the adsorbed species. As the adsorbed molecules absorb electromagnetic radiation corresponding to some unique electronic, vibration or rotational transition, they excite by re-emission or transfer energy into translational mode. This relaxation process increases the surface temperature of the substrate. Since the surface waves are extremely sensitive to temperature, the frequency variation of the surface waves follows the absorption spectra of the particular adsorbed molecules. Other effects include optical-induced stress changes, mass changes due to desorbing molecules, acoustic impedance, density, etc. Additionally, the mass change due to the adsorbed species can also be measured at a reference lighting condition—for example, no light—to gain additional information about the nature of the adsorbed species (for example, amount, presence of additional materials, etc.).
In addition to temperature, the surface wave velocity is affected by pressure, surface stress, magnetic and electric fields. This invention exploits changes in surface wave velocity due to variation in surface temperature, pressure, surface stress, electric and magnetic field induced by interaction of adsorbate with impinging electromagnetic radiation on the sensor surface. A variation in surface acoustic wave properties such as frequency, velocity, phase, amplitude, and Q-factor as a function of the wavelength of the impinging electromagnetic wave is used as a signatures for speciation of adsorbed species.
The surface can be uncoated presenting the native surface to the absorbing species or can be treated to present a more favorable surface for the absorbing species. Such surface treatments can include monolayers assembled onto the surface that can make the surface polar or non-polar, such as is used in chromatographic columns. The treatment can also include a film of material that may be porous or permeable to the absorbing species. Such coating can be used to concentrate the analytes of interest by their partition function, thus increasing the sensitivity of the device. Again, the native surface may be satisfactory for many analytes of interest.
It is well known that molecular adsorption on a SAW surface changes the surface acoustic wave properties. In this invention we expose the adsorbed species to electromagnetic waves where the adsorbate molecules absorb the electromagnetic waves creating localized changes in surface temperature which in turn affect the properties of the surface acoustic waves. The variation in surface wave properties as a function of illuminating wavelength has the unique chemical signature of the adsorbate where the adsorbed species absorb the electromagnetic waves. The surface acoustic wave properties can be frequency, velocity, phase, acoustic impedance and Q-factor. In addition to absorption spectrum of the adsorbates, other physical and chemical properties of the adsorbates can be exploited for obtaining chemical signatures of the adsorbed species. For example, illumination with UV light can induce triplet states or free radicals in certain chemicals producing localized variation in electric and magnetic field intensity which in turn affect the surface acoustic wave properties.
The present invention has a number of embodiments. All the embodiments utilize surface waves. The surface waves can be of different types. The sample surface is illuminated using radiation such as a pulsed light source. Scanning the wavelength allows determination of wavelengths that are absorbed by the sample.
Embodiment I (
Embodiment II: In this embodiment, a thin micromachined membrane is excited by a light source or by electromagnetic induction to create surface waves on the membrane. For example, a micromachined thin-film widow is pulsed with a high intensity focused light source that is localized in size. The sudden change in temperature produces a wave that travels away from the source. The wave can be detected with a high frequency cantilever placed on the membrane, by optical deflection, or by capacitive readout or built-in piezoresistive or piezoelectric readout. Such readout methods are well known. Such a device would be coated (or not) and be exposed to selected wavelengths as described above to obtain chemical selectivity and specificity.
Embodiment III (
Embodiment IV: The fourth embodiment, also shown in
Illumination at energies above band gap produces electron-hole pairs that are separated by electric field in the depletion layer. The bias and semiconductor dopant can be selected in such a way that either electrons or holes will come to the surface. The collection of electrons or holes at the surface forms a two dimensional pool of charge carriers. Surface waves in the two dimensional pool of charge carriers are created by pulsing a focused light on the surface as in Embodiment III or by means of an electrode (generator) placed on the surface. Another electrode (detector) placed around or nearby the first electrode serves as the pickup. In the case of electrode wave generation, a pulsed or modulating voltage applied to the generator electrode to create charge waves rides on the top of the constant bias used for creating the depletion layer.
The invention has been described in terms of specific embodiments which are indicative of a broad utility but are not limitations to the scope of the invention. Additions and modifications apparent to those with skill in the art are included within the scope and spirit of the invention.
This application claims priority to U.S. Provisional Patent Application 60/560,135 filed Apr. 7, 2004, and is herein incorporated by reference.
This invention was made with United States Government support under Contract No. DE-AC05-00OR22725 between the United States Department of Energy and U.T. Battelle, LLC. The United States Government has certain rights in this invention.
| Number | Date | Country | |
|---|---|---|---|
| 60560135 | Apr 2004 | US |
