The present invention belongs to the field of sustained drug release, and provides drug delivery compositions and methods of manufacturing drug delivery compositions.
Naloxone and naltrexone are opioid receptor antagonists. The need for a long-acting opioid receptor antagonist as a treatment for addiction, by blocking the euphoric effects of illicit opioids for an extended period of time, motivated the development of naltrexone. This antagonist has good oral bioavailability, a long duration of action, and twice the potency of a similar compound called naloxone. Naloxone produces essentially no dysphoria but it has a short duration of action and poor oral bioavailability due to high first-pass hepatic metabolism. The half-life of naltrexone can be up to fourteen (14) hours (oral) and the effect can last for a day while naloxone's half-life is only about 30-81 min. (IV, IM) and only lasts for about 1-4 hours.
The difficult-to-treat, chronic pain disorder, fibromyalgia syndrome (FMS) is characterized by diffuse musculoskeletal pain, fatigue, sleep disturbance, and cognitive impairments. Headaches, stomach problems, and a number of other symptoms are also frequently reported. FMS affects 2-8% of the population and predominately women in the United States. The disorder can be debilitating, as the pain and fatigue prevent the individual from carrying out their normal activities.
FMS is seen as a lifelong disease that rarely resolves completely. The FDA has approved four medications for FMS including Amitriptyline (Elavil®), pregabalin (Lyrical)), milnacipran (Savella®), and duloxetine (Cymbalta®), but not all individuals respond well to those treatments. In fact, it is estimated that only about a third of the patients currently respond to these medications. Due to these shortcomings in safety and tolerability of conventional therapies there remains a large clinical unmet need.
One agent, as a potential alternative to naltrexone, is acamprosate (Campral), which may help alcoholics maintain abstinence by preventing relapse. Acamprosate has in vitro affinity for GABA type A and GABA type B receptors, so it's been assumed that the therapeutic effects of acamprosate are due to actions on GABA receptors. However, acamprosate does not share most of the other effects of GABA receptor modifying drugs, such as antianxiety, hypnotic, or muscle relaxant activity. Acamprosate is structurally related to l-glutamic acid (l-glutamate), which is an excitatory neurotransmitter. It has not been extensively studied in chronic pain and cannot be used in patients with chronic renal disease.
In view of the above, there is a need for improving drug-delivery methods and compositions.
According to one embodiment, the present invention provides a drug delivery composition, comprising a microparticulate formulation, said microparticulate formulation further comprising a matrix, a solvent, and at least one low dose opioid receptor antagonist or a combination thereof, wherein said opioid receptor antagonist is incorporated into said matrix homogenously or substantially homogenously.
According to another embodiment, the matrix controls release of said opioid receptor antagonist for initial release rate, daily release rate, duration, and completeness of release, allows release of exact amount of the opioid receptor antagonist, allows sustained release of the opioid receptor antagonist, or a combination thereof.
According to another embodiment, the matrix comprises a solid hydrophobic component and a liquid hydrophobic component.
According to another embodiment, the solvent is phosphate buffered saline or water.
According to another embodiment, the low dose opioid receptor antagonist is selected from group consisting of naltrexone and naloxone.
According to another embodiment, the microparticulate formulation is in a powder form or an injectable form.
According to another embodiment, the present invention provides a method of manufacturing a drug delivery composition, comprising mixing the solid hydrophobic component and the liquid hydrophobic component of the matrix and the opioid receptor antagonist until a microparticulate formulation in a powder form is formed; adding a small amount of water or phosphate buffered saline; and mixing continuously to incorporate the opioid receptor antagonist homogenously or substantially homogenously into the matrix.
According to another embodiment, a method of manufacturing the drug delivery composition comprises adding the solid hydrophobic component and the liquid hydrophobic component of the matrix; mixing said components by kneading, pressing, folding or combination thereof to obtain a kneadable macroscopic mass; combining the macroscopic mass with the opioid receptor antagonist; mixing the macroscopic mass with the opioid receptor antagonist by kneading, pressing, folding or a combination thereof to incorporate the opioid receptor antagonist homogenously into the matrix, thereby payloading the matrix; micronizing the payloaded matrix by adding magnesium stearate to the payloaded matrix and mixing the payloaded matrix and magnesium stearate by kneading, pressing, folding or a combination thereof until a microparticulate formulation in a powder form is formed.
According to another embodiment, a method of manufacturing the drug delivery composition further comprises fractionating the microparticulate formulation by sieving.
According to another embodiment, a method of manufacturing the drug delivery composition further comprises adding the solid hydrophobic component and liquid hydrophobic component of the matrix and the opioid receptor antagonist in a reaction vessel, wherein the solid hydrophobic component is added in a two-fold amount to the mixture; and mixing the components and the opioid receptor antagonist by kneading, pressing, folding, grinding, milling, or a combination thereof so that the microparticulate formulation has a size and size distribution that allows it to be administered as an injectable.
According to another embodiment, a method of manufacturing the drug delivery composition further comprises fractioning the microparticulate formulation by sieving. According to yet another embodiment, said microparticulate formulation is resuspended in an aqueous or an oily suspension. According to yet another embodiment, the microparticulate formulation is prefilled in a syringe in powder form or dissolved in aqueous solution outside of the syringe and then used to fill the syringe. According to yet another embodiment, the prefilled syringe further comprises at least one of a biocompatible oil, saline, and saline with hyaluronic acid.
According to another embodiment, the present invention provides a method of treating an individual with a chronic pain disorder, comprising administering the drug delivery composition to the individual. According to yet another embodiment, the drug delivery composition is administered intramuscularly, intravenously, intradermally, subcutaneously, or via another route.
According to yet another embodiment, administration of the drug delivery composition allows sustained release of the opioid receptor antagonist in the individual's body.
According to another embodiment, the present invention provides a method of treating an individual suffering from or that has been diagnosed with fibromyalgia, Crohn's disease, multiple sclerosis, and pruritus associated with systemic sclerosis.
The accompanying drawings are included to further an understanding of the embodiments that are incorporated in and constitute a part of this specification. The drawings illustrate embodiments and together with the description serve to explain principles of embodiments. Other embodiments and many of the intended advantages of embodiments will be readily appreciated, as they become better understood by reference to the detailed description. The elements of the drawings are not necessarily to scale relative to each other.
The following language and descriptions of certain preferred embodiments of the present invention are provided in order to further an understanding of the principles of the present invention. However, it will be understood that no limitations of the present invention are intended, and that further alterations, modifications, and applications of the principles of the present invention are also included.
In general, the present invention is drawn to a novel, drug delivery platform technology that allows the sustained release delivery of active agents or drugs, without any chemical modification of the drug. The present invention provides significant and unexpected advantages given that current commercially available drug delivery systems have significant limitations. In addition, the technology described herein is also tunable, controlling drug release for initial release rate (burst rate), daily release rate (first derivation of release profile), duration and completeness of release.
Further, unlike many other drug delivery systems, the technology described herein can be put into a standard syringe and injected under the skin (subcutaneously) through a 28 G needle or into a muscle (intramuscularly) using a needle of 22 gauge or smaller and with a volume of less than three (3) milliliters. The matrix systems described herein can be fine-tuned to release the exact amount of drug that is needed on a daily basis avoiding the risk of overdosing and other side effects, and with substantially linear kinetics.
As used herein, with regard to the present invention, “low-dose naltrexone” (LDN) describes the “off-label” use of naltrexone at low doses for diseases not related to chemical dependency or intoxication. Naltrexone, given at low dosages (in the range of about 3-5 mg/day), has been demonstrated to reduce symptom severity in a small number of chronic conditions, including fibromyalgia, Crohn's disease, multiple sclerosis, and pruritus associated with systemic sclerosis. As an orally available compound that is structurally similar to naloxone, naltrexone may work to reduce disease severity by enhancing endogenous endorphin function and suppressing centrally acting proinflammatory cytokines, thereby helping to attenuate pain and other symptoms. This anti-inflammatory effect is distinct from the better-known effect of naltrexone in the blockade of neuronal opioid receptors and may instead involve the antagonism of immune cell receptors, including microglia in the central nervous system. As used herein, with regard to the present invention, “low-dose naltrexone” (LDN) is further intended to refer to novel and non-obvious “off-label” uses of naltrexone at low doses for diseases not related to chemical dependency or intoxication, where there is a significant unmet need for effective treatments.
For the purpose of this specification, the term “mixing” intends to describe, by way of non-limiting example, a mechanical process or a mechanical treatment of the components. Further, by way of non-limiting example, mixing can be in the sense of carrying out repeated cycles of pressing and folding or comparable processing steps which lead to an intense compression and mixing of the components.
In addition to the uses of the matrix systems as described herein for release of naltrexone, the matrix systems of the present invention can reliably and predictably be used for release of naloxone, and for achieving release of the exact amount of naloxone that is needed or desired.
According to the present invention, because the drugs are incorporated into the matrix homogeneously, or substantially homogeneously, even sudden matrix destruction would have a rather moderate effect on release modification. This technology has broad application for multiple biologic agents, small molecule inhibitors, peptides, and low molecular weight drugs.
The present invention provides novel, non-obvious, surprising and unexpectedly beneficial formulations that reliably provide sustained release of an active pharmaceutical agent, such as naltrexone or naloxone, and representative processes and procedures to prepare and obtain such formulations.
According to one embodiment, the present invention provides a drug delivery composition, comprising a microparticulate formulation, said microparticulate formulation further comprising a matrix, a solvent, and at least one low dose opioid receptor antagonist or a combination thereof, wherein said opioid receptor antagonist is incorporated into said matrix homogenously or substantially homogenously.
According to another embodiment, the matrix controls release of said opioid receptor antagonist for initial release rate, daily release rate, duration, and completeness of release, allows release of exact amount of the opioid receptor antagonist, allows sustained release of the opioid receptor antagonist, or a combination thereof.
According to another embodiment, the matrix comprises a solid hydrophobic component and a liquid hydrophobic component.
According to another embodiment, the solvent is phosphate buffered saline or water.
According to another embodiment, the low dose opioid receptor antagonist is selected from group consisting of naltrexone and naloxone.
According to another embodiment, the microparticulate formulation is in a powder form or an injectable form.
According to another embodiment, the present invention provides a method of manufacturing a drug delivery composition, comprising mixing the solid hydrophobic component and the liquid hydrophobic component of the matrix and the opioid receptor antagonist until a microparticulate formulation in a powder form is formed; adding a small amount of water or phosphate buffered saline; and mixing continuously to incorporate the opioid receptor antagonist homogenously or substantially homogenously into the matrix.
According to another embodiment, a method of manufacturing the drug delivery composition comprises adding the solid hydrophobic component and the liquid hydrophobic component of the matrix; mixing said components by kneading, pressing, folding or combination thereof to obtain a kneadable macroscopic mass; combining the macroscopic mass with the opioid receptor antagonist; mixing the macroscopic mass with the opioid receptor antagonist by kneading, pressing, folding or a combination thereof to incorporate the opioid receptor antagonist homogenously into the matrix, thereby payloading the matrix; micronizing the payloaded matrix by adding magnesium stearate to the payloaded matrix and mixing the payloaded matrix and magnesium stearate by kneading, pressing, folding or a combination thereof until a microparticulate formulation in a powder form is formed.
According to another embodiment, a method of manufacturing the drug delivery composition further comprises fractionating the microparticulate formulation by sieving.
According to another embodiment, a method of manufacturing the drug delivery composition further comprises adding the solid hydrophobic component and liquid hydrophobic component of the matrix and the opioid receptor antagonist in a reaction vessel, wherein the solid hydrophobic component is added in a two-fold amount to the mixture; and mixing the components and the opioid receptor antagonist by kneading, pressing, folding, grinding, milling, or a combination thereof so that the microparticulate formulation has a size and size distribution that allows it to be administered as an injectable.
According to another embodiment, a method of manufacturing the drug delivery composition further comprises fractioning the microparticulate formulation by sieving. According to yet another embodiment, said microparticulate formulation is resuspended in an aqueous or an oily suspension. According to yet another embodiment, the microparticulate formulation is prefilled in a syringe in powder form or dissolved in aqueous solution outside of the syringe and then used to fill the syringe. According to yet another embodiment, the prefilled syringe further comprises at least one of a biocompatible oil, saline, and saline with hyaluronic acid.
According to another embodiment, the present invention provides a method of treating an individual with a chronic pain disorder, comprising administering the drug delivery composition to the individual. According to yet another embodiment, the drug delivery composition is administered intramuscularly, intravenously, intradermally, subcutaneously, or via another route.
According to yet another embodiment, administration of the drug delivery composition allows sustained release of the opioid receptor antagonist in the individual's body.
According to another embodiment, the present invention provides a method of treating an individual suffering from or that has been diagnosed with fibromyalgia, Crohn's disease, multiple sclerosis, and pruritus associated with systemic sclerosis.
Now referring to the Figures, by way of non-limiting example,
Referring to another non-limiting example,
The release profile of any low-dose formulation, in accordance with the present invention, can be controlled by several factors including but not limited to, for example, variations of the payload, size and distribution of the matrix, and selection of the excipients.
To achieve a desired result for administration of a low-dose formulation to an individual, the present invention also contemplates that other parameters may be adjusted as needed or desired, including but not limited to injection volume, injection type, syringe type and size, and needle gauge.
For purposes of illustration of the present invention, sample naltrexone release data from several different representative macroscopic matrices and micronized matrices are shown and described below.
Referring to a non-limiting example, Table 1 provides a summary of representative macroscopic matrices, wherein each matrix comprises a naltrexone payload. Table 1 shows specific sample ID numbers for each macroscopic matrix that comprises a naltrexone payload. By way of non-limiting example,
Referring to
Referring to another non-limiting example,
Referring to another non-limiting example,
Referring to another non-limiting example,
Referring to another non-limiting example,
Referring to another non-limiting example,
Referring to another non-limiting example,
Referring to another non-limiting example, Table 2 provides a summary of representative micronized matrices, wherein each matrix comprises a naltrexone payload. Table 2 shows sample ID numbers for each matrix that comprises a naltrexone payload.
Referring to another non-limiting example,
Referring to another non-limiting example,
Referring to another non-limiting example,
In accordance with the present invention, the incorporation of naltrexone into a matrix can be performed by any suitable method(s) including but not limited to melting, mixing, etc. Additional representative, sample data for naltrexone release is shown in
Referring to another non-limiting example,
Referring to another non-limiting example,
Referring to another non-limiting example,
The present invention demonstrates that naltrexone base provides release profiles (in contrast to the hydrochloride form) that meet preferred target criteria.
The sustained release formulations of the present invention provide a number of advantages. For instance, there are a number of surprising advantages of using the oil based formulations of the present invention, including the fact that the injection volume can be reduced to below one milliliter with the oil based formulations.
In accordance with the present invention, it has also been surprisingly discovered that the oil based formulations (with oil as solvent) permits not only a reduction of the injection volume, but this also allows one to use a thinner needle because the rheology is improved. For example, instead of using a 22 Gauge needle for a naltrexone formulation in an aqueous suspension, one can advantageously use a 26 or even 27 Gauge needle for the same particles in a formulation using oil as solvent. A mixture step be may applied as needed to achieve the desired results. This represents another significant advantage of the present invention.
While the examples described herein demonstrate sustained release of naltrexone, it is to be understood that the matrices of the present invention are also suitable for achieving sustained release of naloxone in an amount and for a duration that is needed or desired.
It is contemplated that needle size can also be varied in other embodiments to achieve desired results.
The present invention also contemplates other factors including for example, but not limited to, API incorporation from solution (aqueous or alcohol), adaptation of payload to obtain a variation of release duration, reproducibility, improvements in stability, storage testing, and stress release experiments, all of which further demonstrate the advantages of the invention.
In preferred embodiments, the present invention provides sustained release formulations for low dose naltrexone (LDN) such that the LDN can be administered monthly while releasing about 3.5 mg to about 5.5 mg per day. This dosage of naltrexone is considered to be within an appropriate range for treating pain while avoiding nausea. This is also the accepted dose based on clinical trials with the oral preparation.
The sustained release formulations of the present invention also provide surprising and unexpected pharmacokinetic advantages, including significantly minimizing fluctuations in plasma levels of naltrexone. Preferably, use of the sustained release formulations of the present invention enables one to essentially avoid fluctuations in plasma levels of naltrexone. The sustained release formulations of the present invention also provide better downregulation of the opioid receptors by avoiding fluctuations in plasma levels.
It is also within the scope of the invention that an injectable sustained release low dose naltrexone formulation of the present invention can be administered with approximately one month to three months of linear delivery, providing about 3 mg to about 5 mg daily release.
The present invention also preferably provides microparticle formulations. An exemplary microparticle formulation, and exemplary methods of preparing such formulations, are described below.
Exemplary Naltrexone Formulation Components:
Magnesium stearate
D,L-alpha tocopherol
Naltrexone
Phosphate buffered saline (PBS)
Hyaluronic acid (to improve inj ectability)
Exemplary Protocols for Preparing Naltrexone Formulations:
Exemplary Formula #1—PBS Buffer Containing 0.01% Sodium Azide as Suspension and Release Medium:
Exemplary Formula #2—Microparticulate Powder Mass Formulated in a One-Pot Mechanical Process
Exemplary Formula #3—Basic Matrix Formulation
Exemplary Formula #4—Payloading the Matrix
Exemplary Formula #5—Micronization of the Payloaded Matrix
Exemplary Formula #6—Fractionation if Needed (for Example, Sieving)
Other Exemplary Formulation Procedure
Additional Example of a Sustained Release Low Dose Naltrexone (LDN) Formulation:
The formulations of the present invention can be adjusted as needed or desired to provide desired shelf life, stability, and safety profiles and characteristics (including at pre-clinical and launch stages).
Manufacture of the formulations of the present invention also provides additional advantages. Manufacture can be performed at very low incremental costs; and manufacture can be generally automated. Throughout manufacture, according to preferred embodiments, the biological activity of the API (e.g. naloxone or naltrexone) is not affected. All the manufacturing processes and test methods can be fully validated under QSR (GMP) and CE to ensure purity, dose, and all other properties are fully controlled.
Alternative materials are possible in the formulation process.
The present invention provides a number of additional surprising and unexpected advantages, which are achieved by using the formulations described herein. These advantages include, but are not limited to, addressing and reducing the severity of numerous problems that currently exist with conventional approaches. These problems that currently exist with conventional approaches include, but are not limited to, the following:
(1) Fibromyalgia and chronic pain patients often have very poor compliance with oral medications, and therefore a physician-administered injection, in accordance with the present invention, can significantly improve patient outcomes; and
(2) Many patients on conventional oral LDN (oral low dose naltrexone) report regular nausea and other complications. The LDN formulations of the present invention help reduce these complications.
Therapy decisions about other treatments for a given patient will also be simplified since the LDN (based on administration by the methods of the present invention) will be steady state, or substantially steady-state, in the patient. Also, using an injectable LDN of the present invention, the treating physician will also be in control of the therapy.
Moreover, according to the present invention, the effects of LDN can be improved by going from a daily tablet to a monthly injection. Also, patients who respond well to a one-month formulation can be moved to a two or three-month version, simplifying therapy.
While the present invention can be used for treatment and reducing the severity of symptoms in fibromyalgia, the formulations of the present invention can also be used in the treatment or reducing the severity of other chronic conditions including but not limited to Crohn's disease, multiple sclerosis and pruritus associated systemic sclerosis.
Furthermore, the present invention contemplates that naloxone or naltrexone formulations can be administered by means including but not limited to subcutaneous, intraperitoneal, intramuscular, or transdermal administration, or by other routes.
The present invention also contemplates useful and beneficial sustained release formulations of acamprosate. The present invention also contemplates that acamprosate formulations can be administered by means including but not limited to subcutaneous, intraperitoneal, intramuscular, or transdermal administration.
The foregoing descriptions of certain embodiments of the present invention have been presented for purposes of illustration and description. The embodiments described herein are not intended to be exhaustive or to limit the scope of the present invention in any way.
Number | Date | Country | |
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62215248 | Sep 2015 | US |