Claims
- 1. A method of vaccination of a pig against swine dysentery caused by T.hyodysenteriae infection, comprising the parenteral administration to the pig of an oxygen-treated, non-viable strain of Treponema hyodysenteriae selected from the group consisting of isolate 5380, isolates and strains of T. hyodysenteriae having major abundant proteins with molecular masses of 39 kDa, 37 kDa and 36 kDa, and isolates and strains of T. hyodysenteriae having major abundant proteins with molecular masses of 37 kDa and 36 kDa, said molecular masses being determined by SDS-PAGE gel electrophoresis of a whole cell lysate.
- 2. A method according to claim 1, wherein the strain of T. hyodysenteriae is administered by intra-muscular administration.
- 3. A method according to claim 1, wherein the strain of T. hyodysenteriae is a virulent strain.
- 4. A method according to claim 1, wherein the strain of T. hyodysenteriae is an attenuated strain.
- 5. A method according to claim 3, wherein prior to the administration the T. hyodysenteriae has been held in an oxygen saturated solution, so as to render the T. hyodysenteriae non-viable.
- 6. A method according to claim 1, wherein the strain of T. hyodysenteriae is selected from the group consisting of isolates and strains having characteristic major abundant proteins with molecular masses of 39 kDa, 37 kDa and 36 kDa, as determined by SDS-PAGE gel electrophoresis.
- 7. A method according to claim 1, wherein an adjuvant is combined with the T. hyodysenteriae prior to the administration thereof.
- 8. A method of claim 1, further comprising administering the oxygen-treated, non-viable strain of the T. hyodysenteriae in two doses of approximately 1.times.10.sup.9 T. hyodysenteriae, the second dose being administered 7-14 days after the first dose.
- 9. A method of claim 1, comprising administering the oxygen-treated, non-viable strain of the T. hyodysenteriae in three doses.
- 10. A method according to claim 1, wherein the isolates and strains of T. hyodysenteriae having major abundant proteins with molecular masses of 39 kDa, 37 kDa and 36 kDa are selected from the group consisting of strains ATCC 31287, 31212 and isolate 70A.
- 11. A method according to claim 1, wherein the isolates and strains of T. hyodysenteriae having major abundant proteins with molecular masses of 37 kDa and 36 kDa is the strain ATCC 27164.
- 12. A parenterally administered vaccine composition effective in the immunisation of pigs against swine dysentery caused by T. hyodysenteriae infection which comprises an oxygen-treated, non-viable strain of Treponema hyodysenteriae selected from the group consisting of isolate 5380, isolates of strains of T. hyodysenteriae having major abundant proteins with molecular masses of 39 kDa, 37 kDa and 36 kDa and isolates and strains of T. hyodysenteriae having major abundant proteins with molecular masses of 37 kDa and 36 kDa, said molecular masses being determined by SDS-PAGE gel electrophoresis of a whole cell lysate, and a physiologically acceptable carrier therefor.
- 13. A composition according to claim 12, wherein the strains of T. hyodysenteriae is a virulent strain.
- 14. A composition according to claim 12, wherein the strain of T. hyodysenteriae is an attenuated strain.
- 15. A composition according to claim 12, wherein the T. hyodysenteriae strain has been held in an oxygen-saturated solution, so as to render the strain non-viable.
- 16. A composition according to claim 12, wherein the strain of T. hyodysenteriae is a strain having characteristic major abundant proteins with molecular masses of 39 kDa, 37 kDa and 36 kDa proteins, as determined by SDS-PAGE gel electrophoresis of a whole cell lysate.
- 17. A composition according to claim 12, further comprising Freund's incomplete adjuvant.
- 18. A composition according to claim 12, wherein the isolates and strains T. hyodysenteriae having major abundant proteins with molecular masses of 39 kDa, 37 kDa and 36 kDa are selected from the group consisting of strains ATCC 31287, 31212 and isolate 70A.
- 19. A composition according to claim 12, wherein the isolates and strains of T. hyodysenteriae having major abundant proteins with molecular masses of 37 kDa and 36 kDa is the strain ATCC 27164.
Priority Claims (2)
Number |
Date |
Country |
Kind |
PH9632/86 |
Dec 1986 |
AUX |
|
PI3940/87 |
Aug 1987 |
AUX |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
This is a continuation of my application Ser. No. 07/318,417, filed as PCT/AU87/00440, on Dec. 23, 1987, now abandoned.
US Referenced Citations (10)
Foreign Referenced Citations (7)
Number |
Date |
Country |
340079 |
Feb 1980 |
DKX |
503185 |
Nov 1985 |
DKX |
9577 |
Apr 1980 |
EPX |
0242082 |
Oct 1987 |
EPX |
55-047617 |
Apr 1980 |
JPX |
8602354 |
Apr 1986 |
WOX |
8620354 |
Apr 1986 |
WOX |
Continuations (1)
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Number |
Date |
Country |
Parent |
381417 |
Aug 1989 |
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